Rh1AG447700

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001532
Physical Location & Seq
Reverse (-)
66762226 .. 66765575
3350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG447700.1

Sequence Viewer

Length: 2325 bp
ATGGCGGTGGAGGTCCCGGAGGACGCCCCCGCCCCGGGAGTCGACGGCGACATCGCCGCAGACCTCGATATGCAAGTCTCAGACGACGACGACGACACCTCAGACGGCGAATACGACGACGCCGAGAATCCAGACGACGGCGACGAGCTCATCCACGAAGTCGACAACGACTCGTCGTTGTCGGAGGAAGAGGTGGAGTCCGATGAGACGTGGCTAGAGGAAGCTGAATTCGCCGGAGGGCGCAAGTCCCAAGAGTGTACTTTGATTTTGACACAAGGAATGTCAACGTCAAGTCTCGCACTAAACGGCGTTGTTTCCATGAAGGGTTGGGAAAGGTACGGTGTTTACCCACTGGTAGGTGAATTGCTCAACGTGAGAGAAGCTGGCTTCTTTGAGGTCATGTATAATCCGATTTTCAAGACGATTATGAAGACTCTCGGACTTGAGCTAGATGAAGAATTTACCAGTACCGAATCTTTGAGATATGGCCGGGTCATTATTATGACTGCTGAACAGGATCATTATGGTAGTTACATCAAAGGGTTGTTGATCAATTTTCTTCATATGTTCTGGCCATCATTGCTAAAGCTTCCGTCATTTTTGGCCGAGCTTATAGCTCCTCTTACCATCAAGGTTGGTTGGTTGAGTGAGGAAGGGAAAGAAAAACGTGTATTCTATTCCCTGCAAGAGTATGAAGCTTGGAGGGAAAGTTTGAGGGGCAATGCAAGTGGCTGGGCCATTGAGAAGGATGTAGCATGTGTTTATGATGAAGGGAAGGATTATTTTGAAAATCTTTTAATGCAGAAGAAAGATTTTGTTTGGGTAGATGAACGTGATGATGAGGCAATTGAGCTTGCTTTCAGCAAGAAGTTTGAGGTGGGAAGTTTGCAGTCTGGTATGCATCTTGATCATGAGGAGAAACTTGTAACTTATAGTGACTTTGTTGACAAGGAGCTTATACCCTTTTCCATGCTTGCTGTTCTGCAACGCTCAATTCCGTCAATGGTTGATGGCTTGAAGCCTCATCAAAGAAAGGTTCTATTCAGCTCTTTTAAGAGGGACGACTTTATCAAGGAAGAAATAGTTGCCAGCTTTGCTAATTATGTGGCTGAACACTCGGCTCGTCCTGTGTGTGAGCCCTCAGCGTACCACCATGATGAGCAGAGTCTTGACAGTACAATCATTGAAATGGCCCAGGCTTATGTTGGCAGCAACAACATTAATCTTCTTTTACCAAGAGGTCTATTTGGAAGCCGCCGATTGGGAAGCAAAATCCATGGAAGCGTTCTTCATCTTTCTACTCGACTTTCTCCCATCACAAGGTTCCTTTTTCCAAAGGATGATGACAGGCTTCTTGACTACTTGCATGAAGATGGCAAATCTATTGAGCCAACTTGGTATTTACCAATTATCCCAACGGTTCTTGTTAATGGTAGCGAAGGTATTGGGCCTGCTGGGTGGATCTCTTACATCCCTAGTTATAACCCAAGAGACATTGTGGAAAACGTGAAGCGTTTGATTAGTGATGATAGAATGGTGCCCATGGATCCTTGGTACAGAGGCTTTACCGGAACTATTGAGAAAACTGTTACAGATTCTGGCGTGTGCTACACTACTTGTGGGATCATAAAGAAAGAAGATGAGTCAACACTCAGGATTACGGAGCTGCCAATCGGTACTTGCATTAAAGATTATTTGGAATTTCTTGACTCTATCTCGGAAGTGAATGGTGAGGCCAAGGATCATTGGATTAAGGATTACGAAGATTTTTCCTACATAGATGTTGATATTCTCATCCACTTGTCTGATACTGTGAAGAACTTGACAGAAGCCAGTGAAGAGGATTTGTGTAAGAAGTTTAAACTGACAAGTAGCAGAAGTATAAGTGACATACACCTGTTTGATCCAACAGGCGAGATTAAGAGATATGACACTCCAGAACAAATTCTTGAGGAATTTTTTCATCTAAGACTCCAATTCTATGAGAAAAGAAAGACAGTACTATTGCTTATCCTTAGATCGGAGTTGTCAAATTTGGAAAACAAGGTTGCACTTATGCGTGGTGTCTTAGATGGAGGAGTGGTCGACTTCAATAAACTTAGCACTGATCCGTACCTAAAAAGCATGCCAATTACTACGTTTGTCTACAGAGACAGTTATCAAGAAAAGTTGCAAGAATTATTGGCTGATATAGATAGGATCAAACTGAAGATTAAAAAGTTGGAAGGTTCAACTCCGAGGACATTGTGGTTGGAGGAGCTTGCTGCTCTTGAACAGGAACTTGATAAGCTGGAGAAGCAGAAGCTGTTGGAGGAACTAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 map01524
Pfam Domains
Protein Families
DNA_gyraseB DNA_topoisoIV HATPase_c TOPRIM_C Toprim

Protein Analysis

774

Amino Acids

88.24

Weight (kDa)

4.6

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOPRIM_C PF16898 220 - 325 1.1e-17 C-terminal associated domain of TOPRIM
DNA_topoisoIV PF00521 330 - 687 2.2e-79 DNA gyrase/topoisomerase IV, subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1482
AccB1I GGYRCC 1 cut(s) 1537
AccI GTMKAC 4 cut(s) 42, 162, 2085, 2145
AciI CCGC 4 cut(s) 5, 30, 57, 1255
AclWI GGATC 9 cut(s) 525, 1469, 1541, 1554, 1631, 1749, 1898, 2102, 2207
AcoI YGGCCR 3 cut(s) 487, 572, 603
AcsI RAATTY 6 cut(s) 227, 458, 1700, 1944, 1955, 2032
AcuI CTGAAG 1 cut(s) 2228
AcyI GRCGYC 2 cut(s) 24, 120
AfaI GTAC 9 cut(s) 259, 338, 469, 1148, 1177, 1556, 1678, 2001, 2114
AfiI CCNNNNNNNGG 7 cut(s) 34, 35, 137, 356, 1261, 1320, 2020
AflIII ACRYGT 1 cut(s) 667
AgsI TTSAA 7 cut(s) 418, 788, 1018, 1187, 2092, 2232, 2273
AjiI CACGTC 1 cut(s) 210
AjnI CCWGG 1 cut(s) 1194
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 5 cut(s) 82, 200, 299, 1485, 2145
AlwI GGATC 9 cut(s) 525, 1469, 1541, 1554, 1631, 1749, 1898, 2102, 2207
AlwNI CAGNNNCTG 2 cut(s) 1598, 2305
Ama87I CYCGRG 1 cut(s) 34
AoxI GGCC 7 cut(s) 487, 572, 603, 735, 1191, 1448, 1734
ApeKI GCWGC 3 cut(s) 1209, 1666, 2264
ApoI RAATTY 6 cut(s) 227, 458, 1700, 1944, 1955, 2032
AseI ATTAAT 1 cut(s) 1221
Asp700I GAANNNNTTC 2 cut(s) 1944, 1959
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 4 cut(s) 13, 735, 1192, 1448
AsuC2I CCSGG 4 cut(s) 17, 35, 36, 491
AsuHPI GGTGA 2 cut(s) 371, 1742
AvaI CYCGRG 1 cut(s) 34
AvaII GGWCC 1 cut(s) 13
BaeGI GKGCMC 1 cut(s) 1542
BalI TGGCCA 1 cut(s) 574
BamHI GGATCC 1 cut(s) 1546
BanI GGYRCC 1 cut(s) 1537
BanII GRGCYC 2 cut(s) 150, 1140
BbsI GAAGAC 1 cut(s) 437
Bbv12I GWGCWC 1 cut(s) 150
BbvCI CCTCAGC 1 cut(s) 1141
BbvI GCAGC 3 cut(s) 1221, 1653, 2251
BccI CCATC 6 cut(s) 583, 635, 1004, 1322, 1367, 2066
BceAI ACGGC 4 cut(s) 61, 121, 154, 322
BciT130I CCWGG 1 cut(s) 1196
BclI TGATCA 2 cut(s) 549, 907
BcnI CCSGG 4 cut(s) 17, 35, 36, 491
BcoDI GTCTC 5 cut(s) 82, 200, 299, 1485, 2145
BfaI CTAG 4 cut(s) 215, 449, 1476, 2318
BfmI CTRYAG 1 cut(s) 2146
BisI GCNGC 5 cut(s) 57, 1210, 1255, 1667, 2265
BlsI GCNGC 5 cut(s) 58, 1211, 1256, 1668, 2266
BmcAI AGTACT 1 cut(s) 2001
Bme1390I CCNGG 5 cut(s) 17, 35, 36, 491, 1196
Bme18I GGWCC 1 cut(s) 13
BmeT110I CYCGRG 1 cut(s) 34
BmgBI CACGTC 1 cut(s) 210
BmgT120I GGNCC 4 cut(s) 13, 735, 1192, 1448
BmiI GGNNCC 4 cut(s) 15, 1325, 1539, 1548
BmrFI CCNGG 5 cut(s) 17, 35, 36, 491, 1196
BmsI GCATC 1 cut(s) 910
BpiI GAAGAC 1 cut(s) 437
BpmI CTGGAG 2 cut(s) 1920, 2312
Bpu10I CCTNAGC 1 cut(s) 1141
BpuEI CTTGAG 2 cut(s) 464, 1970
BpuMI CCSGG 4 cut(s) 17, 35, 36, 491
BsaHI GRCGYC 2 cut(s) 24, 120
BsaJI CCNNGG 8 cut(s) 33, 34, 1194, 1276, 1542, 1550, 1737, 2237
BsaWI WCCGGW 1 cut(s) 1568
BsaXI ACNNNNNCTCC 4 cut(s) 694, 724, 2067, 2097
Bsc4I CCNNNNNNNGG 7 cut(s) 34, 35, 137, 356, 1261, 1320, 2020
Bse1I ACTGG 3 cut(s) 357, 465, 1833
Bse3DI GCAATG 2 cut(s) 578, 727
BseBI CCWGG 1 cut(s) 1196
BseDI CCNNGG 8 cut(s) 33, 34, 1194, 1276, 1542, 1550, 1737, 2237
BseGI GGATG 5 cut(s) 150, 754, 1345, 1470, 1794
BseLI CCNNNNNNNGG 7 cut(s) 34, 35, 137, 356, 1261, 1320, 2020
BseMI GCAATG 2 cut(s) 578, 727
BseMII CTCAG 4 cut(s) 93, 114, 1155, 1666
BseNI ACTGG 3 cut(s) 357, 465, 1833
BseRI GAGGAG 4 cut(s) 609, 929, 2091, 2270
BseSI GKGCMC 1 cut(s) 1542
BseXI GCAGC 3 cut(s) 1221, 1653, 2251
BseYI CCCAGC 2 cut(s) 732, 1454
BshFI GGCC 7 cut(s) 489, 574, 605, 737, 1193, 1450, 1736
BshNI GGYRCC 1 cut(s) 1537
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 1 cut(s) 34
BsiSI CCGG 5 cut(s) 17, 35, 234, 490, 1569
BslFI GGGAC 2 cut(s) 232, 1073
BslI CCNNNNNNNGG 7 cut(s) 34, 35, 137, 356, 1261, 1320, 2020
BsmAI GTCTC 5 cut(s) 82, 200, 299, 1485, 2145
BsmBI CGTCTC 1 cut(s) 200
BsmFI GGGAC 2 cut(s) 232, 1073
BsnI GGCC 7 cut(s) 489, 574, 605, 737, 1193, 1450, 1736
BsoBI CYCGRG 1 cut(s) 34
Bsp1286I GDGCHC 3 cut(s) 150, 1140, 1542
Bsp19I CCATGG 2 cut(s) 1276, 1542
BspACI CCGC 4 cut(s) 5, 30, 57, 1255
BspANI GGCC 7 cut(s) 489, 574, 605, 737, 1193, 1450, 1736
BspCNI CTCAG 4 cut(s) 92, 113, 1154, 1665
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 4 cut(s) 15, 1325, 1539, 1548
BspPI GGATC 9 cut(s) 525, 1469, 1541, 1554, 1631, 1749, 1898, 2102, 2207
BspT107I GGYRCC 1 cut(s) 1537
BsrDI GCAATG 2 cut(s) 578, 727
BsrI ACTGG 3 cut(s) 357, 465, 1833
BssECI CCNNGG 8 cut(s) 33, 34, 1194, 1276, 1542, 1550, 1737, 2237
BssNI GRCGYC 2 cut(s) 24, 120
BssT1I CCWWGG 4 cut(s) 1276, 1542, 1550, 1737
Bst2UI CCWGG 1 cut(s) 1196
Bst4CI ACNGT 7 cut(s) 341, 1175, 1420, 1588, 1813, 1999, 2156
Bst6I CTCTTC 2 cut(s) 183, 1833
BstACI GRCGYC 2 cut(s) 24, 120
BstC8I GCNNGC 7 cut(s) 385, 855, 975, 1090, 1452, 2126, 2262
BstDEI CTNAG 8 cut(s) 79, 100, 1141, 1652, 1967, 2015, 2068, 2099
BstDSI CCRYGG 2 cut(s) 1276, 1542
BstF5I GGATG 5 cut(s) 150, 754, 1345, 1470, 1794
BstHHI GCGC 1 cut(s) 243
BstMAI GTCTC 5 cut(s) 82, 200, 299, 1485, 2145
BstMWI GCNNNNNNNGC 4 cut(s) 230, 580, 1094, 2296
BstNI CCWGG 1 cut(s) 1196
BstNSI RCATGY 2 cut(s) 759, 2128
BstSCI CCNGG 5 cut(s) 15, 33, 34, 489, 1194
BstSFI CTRYAG 1 cut(s) 2146
BstSLI GKGCMC 1 cut(s) 1542
BstV1I GCAGC 3 cut(s) 1221, 1653, 2251
BstV2I GAAGAC 1 cut(s) 437
BstX2I RGATCY 2 cut(s) 1461, 1546
BstYI RGATCY 2 cut(s) 1461, 1546
BsuRI GGCC 7 cut(s) 489, 574, 605, 737, 1193, 1450, 1736
BtgI CCRYGG 2 cut(s) 1276, 1542
BtgZI GCGATG 1 cut(s) 37
BtrI CACGTC 1 cut(s) 210
BtsCI GGATG 5 cut(s) 150, 754, 1345, 1470, 1794
BtsIMutI CAGTG 3 cut(s) 350, 1840, 2103
Cac8I GCNNGC 7 cut(s) 385, 855, 975, 1090, 1452, 2126, 2262
CaiI CAGNNNCTG 2 cut(s) 1598, 2305
CciI TCATGA 1 cut(s) 910
CfoI GCGC 1 cut(s) 243
Cfr13I GGNCC 4 cut(s) 13, 735, 1192, 1448
Cfr9I CCCGGG 1 cut(s) 34
CseI GACGC 2 cut(s) 32, 128
Csp6I GTAC 9 cut(s) 258, 337, 468, 1147, 1176, 1555, 1677, 2000, 2113
CspCI CAANNNNNGTGG 2 cut(s) 709, 744
CviQI GTAC 9 cut(s) 258, 337, 468, 1147, 1176, 1555, 1677, 2000, 2113
DdeI CTNAG 8 cut(s) 79, 100, 1141, 1652, 1967, 2015, 2068, 2099
DraI TTTAAA 1 cut(s) 1861
EaeI YGGCCR 3 cut(s) 487, 572, 603
Eam1104I CTCTTC 2 cut(s) 183, 1833
EarI CTCTTC 2 cut(s) 183, 1833
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 4 cut(s) 1276, 1542, 1550, 1737
Eco24I GRGCYC 2 cut(s) 150, 1140
Eco47I GGWCC 1 cut(s) 13
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 2228
Eco88I CYCGRG 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 148
EcoO109I RGGNCCY 1 cut(s) 13
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 1194
EcoT14I CCWWGG 4 cut(s) 1276, 1542, 1550, 1737
EcoT22I ATGCAT 1 cut(s) 903
EcoT38I GRGCYC 2 cut(s) 150, 1140
ErhI CCWWGG 4 cut(s) 1276, 1542, 1550, 1737
Esp3I CGTCTC 1 cut(s) 200
FaqI GGGAC 2 cut(s) 232, 1073
FauI CCCGC 1 cut(s) 37
FauNDI CATATG 1 cut(s) 564
FbaI TGATCA 2 cut(s) 549, 907
FblI GTMKAC 4 cut(s) 42, 162, 2085, 2145
Fnu4HI GCNGC 5 cut(s) 57, 1210, 1255, 1667, 2265
FokI GGATG 5 cut(s) 137, 761, 1352, 1457, 1781
FriOI GRGCYC 2 cut(s) 150, 1140
Fsp4HI GCNGC 5 cut(s) 57, 1210, 1255, 1667, 2265
FspBI CTAG 4 cut(s) 215, 449, 1476, 2318
GlaI GCGC 1 cut(s) 242
GluI GCNGC 5 cut(s) 57, 1210, 1255, 1667, 2265
GsaI CCCAGC 2 cut(s) 736, 1458
GsuI CTGGAG 2 cut(s) 1920, 2312
HaeIII GGCC 7 cut(s) 489, 574, 605, 737, 1193, 1450, 1736
HapII CCGG 5 cut(s) 17, 35, 234, 490, 1569
HgaI GACGC 2 cut(s) 32, 128
HhaI GCGC 1 cut(s) 243
Hin1I GRCGYC 2 cut(s) 24, 120
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HincII GTYRAC 6 cut(s) 43, 163, 285, 946, 1647, 2086
HindII GTYRAC 6 cut(s) 43, 163, 285, 946, 1647, 2086
HindIII AAGCTT 2 cut(s) 587, 696
HpaII CCGG 5 cut(s) 17, 35, 234, 490, 1569
HphI GGTGA 2 cut(s) 371, 1742
Hpy166II GTNNAC 9 cut(s) 43, 163, 258, 285, 346, 946, 1647, 2086, 2146
Hpy8I GTNNAC 9 cut(s) 43, 163, 258, 285, 346, 946, 1647, 2086, 2146
Hpy99I CGWCG 9 cut(s) 47, 89, 92, 95, 119, 122, 140, 146, 178
HpyAV CCTTC 7 cut(s) 316, 647, 739, 764, 769, 1433, 2219
HpyCH4III ACNGT 7 cut(s) 341, 1175, 1420, 1588, 1813, 1999, 2156
HpyCH4IV ACGT 7 cut(s) 209, 287, 372, 667, 832, 1506, 2138
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 580, 1094, 2296
HpyF3I CTNAG 8 cut(s) 79, 100, 1141, 1652, 1967, 2015, 2068, 2099
HpySE526I ACGT 7 cut(s) 209, 287, 372, 667, 832, 1506, 2138
Hsp92I GRCGYC 2 cut(s) 24, 120
HspAI GCGC 1 cut(s) 241
Ksp22I TGATCA 2 cut(s) 549, 907
LmnI GCTCC 4 cut(s) 622, 952, 1663, 2257
Lsp1109I GCAGC 3 cut(s) 1221, 1653, 2251
LweI GCATC 1 cut(s) 910
MaeI CTAG 4 cut(s) 215, 449, 1476, 2318
MaeII ACGT 7 cut(s) 209, 287, 372, 667, 832, 1506, 2138
MaeIII GTNAC 5 cut(s) 530, 925, 935, 1588, 1886
MfeI CAATTG 1 cut(s) 846
MflI RGATCY 2 cut(s) 1461, 1546
MhlI GDGCHC 3 cut(s) 150, 1140, 1542
MlsI TGGCCA 1 cut(s) 574
MluNI TGGCCA 1 cut(s) 574
MlyI GAGTC 8 cut(s) 48, 164, 206, 427, 1174, 1652, 1703, 1965
MmeI TCCRAC 5 cut(s) 162, 1931, 2202, 2232, 2289
Mox20I TGGCCA 1 cut(s) 574
Mph1103I ATGCAT 1 cut(s) 903
MroXI GAANNNNTTC 2 cut(s) 1944, 1959
MscI TGGCCA 1 cut(s) 574
MseI TTAA 9 cut(s) 797, 1053, 1221, 1428, 1686, 1752, 1860, 1920, 2214
MslI CAYNNNNRTG 4 cut(s) 500, 1155, 1187, 1371
Msp20I TGGCCA 1 cut(s) 574
MspI CCGG 5 cut(s) 17, 35, 234, 490, 1569
MspR9I CCNGG 5 cut(s) 17, 35, 36, 491, 1196
MssI GTTTAAAC 1 cut(s) 1861
MunI CAATTG 1 cut(s) 846
MvaI CCWGG 1 cut(s) 1196
MwoI GCNNNNNNNGC 4 cut(s) 230, 580, 1094, 2296
NciI CCSGG 4 cut(s) 17, 35, 36, 491
NcoI CCATGG 2 cut(s) 1276, 1542
NdeI CATATG 1 cut(s) 564
NlaIV GGNNCC 4 cut(s) 15, 1325, 1539, 1548
NmeAIII GCCGAG 3 cut(s) 148, 631, 1097
NmuCI GTSAC 2 cut(s) 935, 1886
NsiI ATGCAT 1 cut(s) 903
NspI RCATGY 2 cut(s) 759, 2128
PaeI GCATGC 1 cut(s) 2128
PagI TCATGA 1 cut(s) 910
PcsI WCGNNNNNNNCGW 4 cut(s) 90, 111, 120, 141
PdmI GAANNNNTTC 2 cut(s) 1944, 1959
PfeI GAWTC 3 cut(s) 127, 473, 1595
PfoI TCCNGGA 1 cut(s) 15
PkrI GCNGC 5 cut(s) 58, 1211, 1256, 1668, 2266
PleI GAGTC 8 cut(s) 47, 164, 205, 427, 1173, 1651, 1703, 1965
PmeI GTTTAAAC 1 cut(s) 1861
PpsI GAGTC 8 cut(s) 47, 164, 205, 427, 1173, 1651, 1703, 1965
PpuMI RGGWCCY 1 cut(s) 13
PshBI ATTAAT 1 cut(s) 1221
PsiI TTATAA 1 cut(s) 1482
Psp124BI GAGCTC 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 1194
PspFI CCCAGC 2 cut(s) 732, 1454
PspGI CCWGG 1 cut(s) 1194
PspN4I GGNNCC 4 cut(s) 15, 1325, 1539, 1548
PspPI GGNCC 4 cut(s) 13, 735, 1192, 1448
PspPPI RGGWCCY 1 cut(s) 13
PstNI CAGNNNCTG 2 cut(s) 1598, 2305
PsuI RGATCY 2 cut(s) 1461, 1546
RsaI GTAC 9 cut(s) 259, 338, 469, 1148, 1177, 1556, 1678, 2001, 2114
RsaNI GTAC 9 cut(s) 258, 337, 468, 1147, 1176, 1555, 1677, 2000, 2113
RseI CAYNNNNRTG 4 cut(s) 500, 1155, 1187, 1371
SacI GAGCTC 1 cut(s) 150
SalI GTCGAC 3 cut(s) 41, 161, 2084
SaqAI TTAA 9 cut(s) 797, 1053, 1221, 1428, 1686, 1752, 1860, 1920, 2214
SatI GCNGC 5 cut(s) 57, 1210, 1255, 1667, 2265
Sau96I GGNCC 4 cut(s) 13, 735, 1192, 1448
ScaI AGTACT 1 cut(s) 2001
SchI GAGTC 8 cut(s) 48, 164, 206, 427, 1174, 1652, 1703, 1965
ScrFI CCNGG 5 cut(s) 17, 35, 36, 491, 1196
SduI GDGCHC 3 cut(s) 150, 1140, 1542
SfaNI GCATC 1 cut(s) 910
SfcI CTRYAG 1 cut(s) 2146
SinI GGWCC 1 cut(s) 13
SmaI CCCGGG 1 cut(s) 36
SmiMI CAYNNNNRTG 4 cut(s) 500, 1155, 1187, 1371
SmlI CTYRAG 2 cut(s) 443, 1949
SmoI CTYRAG 2 cut(s) 443, 1949
SphI GCATGC 1 cut(s) 2128
SsiI CCGC 4 cut(s) 5, 30, 57, 1255
SspMI CTAG 4 cut(s) 215, 449, 1476, 2318
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 5 cut(s) 15, 33, 34, 489, 1194
StyI CCWWGG 4 cut(s) 1276, 1542, 1550, 1737
TaaI ACNGT 7 cut(s) 341, 1175, 1420, 1588, 1813, 1999, 2156
TaiI ACGT 7 cut(s) 212, 290, 375, 670, 835, 1509, 2141
TaqI TCGA 5 cut(s) 42, 66, 162, 1303, 2085
TatI WGTACW 3 cut(s) 257, 1175, 1999
TauI GCSGC 2 cut(s) 59, 1257
TfiI GAWTC 3 cut(s) 127, 473, 1595
Tru1I TTAA 9 cut(s) 797, 1053, 1221, 1428, 1686, 1752, 1860, 1920, 2214
Tru9I TTAA 9 cut(s) 797, 1053, 1221, 1428, 1686, 1752, 1860, 1920, 2214
TscAI CASTG 3 cut(s) 357, 1840, 2110
TseFI GTSAC 2 cut(s) 935, 1886
TseI GCWGC 3 cut(s) 1209, 1666, 2264
Tsp45I GTSAC 2 cut(s) 935, 1886
TspGWI ACGGA 4 cut(s) 582, 987, 1676, 2100
TspMI CCCGGG 1 cut(s) 34
TspRI CASTG 3 cut(s) 357, 1840, 2110
VpaK11BI GGWCC 1 cut(s) 13
VspI ATTAAT 1 cut(s) 1221
XapI RAATTY 6 cut(s) 227, 458, 1700, 1944, 1955, 2032
XceI RCATGY 2 cut(s) 759, 2128
XcmI CCANNNNNNNNNTGG 1 cut(s) 1202
XmaI CCCGGG 1 cut(s) 34
XmiI GTMKAC 4 cut(s) 42, 162, 2085, 2145
XmnI GAANNNNTTC 2 cut(s) 1944, 1959
XspI CTAG 4 cut(s) 215, 449, 1476, 2318
ZrmI AGTACT 1 cut(s) 2001
Zsp2I ATGCAT 1 cut(s) 903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.