Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| HATPase_c | PF02518 | 79 - 173 | 2.8e-07 | Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase |
| DNA_gyraseB | PF00204 | 269 - 425 | 1.3e-24 | DNA gyrase B |
| Toprim | PF01751 | 455 - 554 | 9e-09 | Toprim domain |
| TOPRIM_C | PF16898 | 570 - 697 | 1.1e-51 | C-terminal associated domain of TOPRIM |
| DNA_topoisoIV | PF00521 | 700 - 1159 | 2.3e-118 | DNA gyrase/topoisomerase IV, subunit A |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 4 cut(s) | 1712, 2562, 2775, 3876 |
| AarI | CACCTGC | 1 cut(s) | 562 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 206 |
| AatII | GACGTC | 1 cut(s) | 330 |
| Acc36I | ACCTGC | 3 cut(s) | 562, 3415, 3722 |
| AccB1I | GGYRCC | 4 cut(s) | 68, 426, 494, 2617 |
| AccII | CGCG | 1 cut(s) | 1490 |
| AclI | AACGTT | 1 cut(s) | 2586 |
| AcoI | YGGCCR | 1 cut(s) | 73 |
| AcsI | RAATTY | 6 cut(s) | 524, 674, 2780, 2871, 3021, 3053 |
| AcuI | CTGAAG | 3 cut(s) | 1289, 2948, 3291 |
| AcyI | GRCGYC | 3 cut(s) | 69, 327, 495 |
| AdeI | CACNNNGTG | 1 cut(s) | 1066 |
| AfeI | AGCGCT | 1 cut(s) | 215 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 391, 1132, 1457, 3097, 3142, 3381 |
| AgeI | ACCGGT | 2 cut(s) | 473, 607 |
| AhlI | ACTAGT | 1 cut(s) | 437 |
| AjnI | CCWGG | 5 cut(s) | 387, 2005, 2131, 2477, 3853 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 3065, 3097, 3294, 3326 |
| AleI | CACNNNNGTG | 1 cut(s) | 2238 |
| Alw21I | GWGCWC | 4 cut(s) | 126, 2371, 2546, 3201 |
| Alw26I | GTCTC | 4 cut(s) | 226, 914, 2565, 2717 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3305 |
| Ama87I | CYCGRG | 2 cut(s) | 645, 1772 |
| Aor51HI | AGCGCT | 1 cut(s) | 215 |
| AoxI | GGCC | 4 cut(s) | 73, 235, 1670, 2814 |
| ApeKI | GCWGC | 8 cut(s) | 25, 33, 286, 1546, 1642, 2906, 3725, 3830 |
| ApoI | RAATTY | 6 cut(s) | 524, 674, 2780, 2871, 3021, 3053 |
| AseI | ATTAAT | 1 cut(s) | 2304 |
| AsiGI | ACCGGT | 2 cut(s) | 473, 607 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 1230, 1907, 3021, 3212 |
| AspLEI | GCGC | 6 cut(s) | 21, 71, 216, 497, 1490, 3574 |
| AspS9I | GGNCC | 7 cut(s) | 208, 642, 787, 1432, 1671, 1821, 2681 |
| AsuC2I | CCSGG | 3 cut(s) | 234, 646, 647 |
| AsuHPI | GGTGA | 8 cut(s) | 310, 472, 661, 2252, 2618, 3032, 3157, 3721 |
| AvaI | CYCGRG | 2 cut(s) | 645, 1772 |
| AvaII | GGWCC | 6 cut(s) | 208, 642, 787, 1432, 1821, 2681 |
| AxyI | CCTNAGG | 2 cut(s) | 2889, 3702 |
| BaeGI | GKGCMC | 2 cut(s) | 429, 2960 |
| BamHI | GGATCC | 3 cut(s) | 155, 2626, 2821 |
| BanI | GGYRCC | 4 cut(s) | 68, 426, 494, 2617 |
| BanII | GRGCYC | 2 cut(s) | 2546, 3201 |
| BbsI | GAAGAC | 3 cut(s) | 181, 473, 1223 |
| Bbv12I | GWGCWC | 4 cut(s) | 126, 2371, 2546, 3201 |
| BbvI | GCAGC | 8 cut(s) | 12, 45, 298, 1558, 1629, 2918, 3737, 3817 |
| BceAI | ACGGC | 7 cut(s) | 66, 82, 94, 376, 502, 508, 562 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 70, 104 |
| BciT130I | CCWGG | 5 cut(s) | 389, 2007, 2133, 2479, 3855 |
| BclI | TGATCA | 4 cut(s) | 1599, 1647, 2020, 2854 |
| BcnI | CCSGG | 3 cut(s) | 234, 646, 647 |
| BcoDI | GTCTC | 4 cut(s) | 226, 914, 2565, 2717 |
| BcuI | ACTAGT | 1 cut(s) | 437 |
| BfaI | CTAG | 4 cut(s) | 438, 1889, 2876, 2949 |
| BfmI | CTRYAG | 3 cut(s) | 26, 1544, 2865 |
| BfoI | RGCGCY | 4 cut(s) | 22, 72, 217, 498 |
| BfuAI | ACCTGC | 3 cut(s) | 562, 3415, 3722 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 50 |
| BglII | AGATCT | 2 cut(s) | 246, 1264 |
| BmcAI | AGTACT | 3 cut(s) | 1855, 2260, 2863 |
| Bme1390I | CCNGG | 8 cut(s) | 234, 389, 646, 647, 2007, 2133, 2479, 3855 |
| Bme18I | GGWCC | 6 cut(s) | 208, 642, 787, 1432, 1821, 2681 |
| BmeT110I | CYCGRG | 2 cut(s) | 645, 1772 |
| BmgT120I | GGNCC | 7 cut(s) | 208, 642, 787, 1432, 1671, 1821, 2681 |
| BmrFI | CCNGG | 8 cut(s) | 234, 389, 646, 647, 2007, 2133, 2479, 3855 |
| BmrI | ACTGGG | 1 cut(s) | 2191 |
| BmsI | GCATC | 7 cut(s) | 351, 734, 1315, 1600, 2980, 3469, 3896 |
| BmuI | ACTGGG | 1 cut(s) | 2191 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1496 |
| BpiI | GAAGAC | 3 cut(s) | 181, 473, 1223 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2780, 2812 |
| BpmI | CTGGAG | 5 cut(s) | 140, 1340, 2807, 3527, 3837 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1392, 3603 |
| BpuEI | CTTGAG | 9 cut(s) | 296, 1037, 1479, 1819, 2751, 2784, 3047, 3113, 3215 |
| BpuMI | CCSGG | 3 cut(s) | 234, 646, 647 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1614 |
| BsaHI | GRCGYC | 3 cut(s) | 69, 327, 495 |
| BsaWI | WCCGGW | 3 cut(s) | 373, 473, 607 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 360, 390, 1610, 1640 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 391, 1132, 1457, 3097, 3142, 3381 |
| Bse118I | RCCGGY | 3 cut(s) | 71, 473, 607 |
| Bse1I | ACTGG | 9 cut(s) | 644, 1419, 2197, 2256, 2489, 2653, 2683, 3672, 3831 |
| Bse21I | CCTNAGG | 2 cut(s) | 2889, 3702 |
| Bse3DI | GCAATG | 3 cut(s) | 951, 1410, 3687 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1614 |
| BseBI | CCWGG | 5 cut(s) | 389, 2007, 2133, 2479, 3855 |
| BseGI | GGATG | 8 cut(s) | 749, 873, 1330, 1936, 2428, 2550, 3132, 3911 |
| BseJI | GATNNNNATC | 1 cut(s) | 1614 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 391, 1132, 1457, 3097, 3142, 3381 |
| BseMI | GCAATG | 3 cut(s) | 951, 1410, 3687 |
| BseMII | CTCAG | 9 cut(s) | 864, 925, 1368, 1748, 2208, 2746, 2880, 3312, 3693 |
| BseNI | ACTGG | 9 cut(s) | 644, 1419, 2197, 2256, 2489, 2653, 2683, 3672, 3831 |
| BseRI | GAGGAG | 3 cut(s) | 407, 2906, 3044 |
| BseSI | GKGCMC | 2 cut(s) | 429, 2960 |
| BseXI | GCAGC | 8 cut(s) | 12, 45, 298, 1558, 1629, 2918, 3737, 3817 |
| BseYI | CCCAGC | 1 cut(s) | 3260 |
| BsgI | GTGCAG | 1 cut(s) | 3744 |
| Bsh1236I | CGCG | 1 cut(s) | 1490 |
| Bsh1285I | CGRYCG | 1 cut(s) | 90 |
| BshFI | GGCC | 4 cut(s) | 75, 237, 1672, 2816 |
| BshNI | GGYRCC | 4 cut(s) | 68, 426, 494, 2617 |
| BshTI | ACCGGT | 2 cut(s) | 473, 607 |
| BsiEI | CGRYCG | 1 cut(s) | 90 |
| BsiHKAI | GWGCWC | 4 cut(s) | 126, 2371, 2546, 3201 |
| BsiHKCI | CYCGRG | 2 cut(s) | 645, 1772 |
| BsiSI | CCGG | 8 cut(s) | 72, 234, 374, 410, 474, 608, 646, 837 |
| BslFI | GGGAC | 7 cut(s) | 215, 773, 1425, 1445, 1886, 2347, 2545 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 391, 1132, 1457, 3097, 3142, 3381 |
| BsmAI | GTCTC | 4 cut(s) | 226, 914, 2565, 2717 |
| BsmBI | CGTCTC | 1 cut(s) | 226 |
| BsmFI | GGGAC | 7 cut(s) | 215, 773, 1425, 1445, 1886, 2347, 2545 |
| BsmI | GAATGC | 2 cut(s) | 2221, 3574 |
| BsnI | GGCC | 4 cut(s) | 75, 237, 1672, 2816 |
| BsoBI | CYCGRG | 2 cut(s) | 645, 1772 |
| Bsp1286I | GDGCHC | 6 cut(s) | 126, 429, 2371, 2546, 2960, 3201 |
| Bsp1407I | TGTACA | 1 cut(s) | 351 |
| Bsp19I | CCATGG | 4 cut(s) | 300, 2082, 2236, 3354 |
| BspANI | GGCC | 4 cut(s) | 75, 237, 1672, 2816 |
| BspCNI | CTCAG | 9 cut(s) | 865, 924, 1369, 1747, 2209, 2745, 2881, 3311, 3694 |
| BspFNI | CGCG | 1 cut(s) | 1490 |
| BspHI | TCATGA | 1 cut(s) | 1602 |
| BspMAI | CTGCAG | 2 cut(s) | 30, 1548 |
| BspMI | ACCTGC | 3 cut(s) | 562, 3415, 3722 |
| BspQI | GCTCTTC | 1 cut(s) | 104 |
| BspT107I | GGYRCC | 4 cut(s) | 68, 426, 494, 2617 |
| BsrDI | GCAATG | 3 cut(s) | 951, 1410, 3687 |
| BsrFI | RCCGGY | 3 cut(s) | 71, 473, 607 |
| BsrGI | TGTACA | 1 cut(s) | 351 |
| BsrI | ACTGG | 9 cut(s) | 644, 1419, 2197, 2256, 2489, 2653, 2683, 3672, 3831 |
| BssAI | RCCGGY | 3 cut(s) | 71, 473, 607 |
| BssNI | GRCGYC | 3 cut(s) | 69, 327, 495 |
| BssT1I | CCWWGG | 9 cut(s) | 300, 748, 863, 2082, 2236, 2630, 2817, 3136, 3354 |
| Bst2UI | CCWGG | 5 cut(s) | 389, 2007, 2133, 2479, 3855 |
| Bst6I | CTCTTC | 5 cut(s) | 104, 827, 1968, 3170, 3285 |
| BstACI | GRCGYC | 3 cut(s) | 69, 327, 495 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 130, 2192 |
| BstAUI | TGTACA | 1 cut(s) | 351 |
| BstC8I | GCNNGC | 6 cut(s) | 23, 73, 116, 551, 1486, 3762 |
| BstDSI | CCRYGG | 5 cut(s) | 76, 300, 2082, 2236, 3354 |
| BstEII | GGTNACC | 1 cut(s) | 316 |
| BstF5I | GGATG | 8 cut(s) | 749, 873, 1330, 1936, 2428, 2550, 3132, 3911 |
| BstFNI | CGCG | 1 cut(s) | 1490 |
| BstH2I | RGCGCY | 4 cut(s) | 22, 72, 217, 498 |
| BstHHI | GCGC | 6 cut(s) | 21, 71, 216, 497, 1490, 3574 |
| BstMAI | GTCTC | 4 cut(s) | 226, 914, 2565, 2717 |
| BstMCI | CGRYCG | 1 cut(s) | 90 |
| BstNI | CCWGG | 5 cut(s) | 389, 2007, 2133, 2479, 3855 |
| BstNSI | RCATGY | 1 cut(s) | 2971 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1496 |
| BstPI | GGTNACC | 1 cut(s) | 316 |
| BstSCI | CCNGG | 8 cut(s) | 232, 387, 644, 645, 2005, 2131, 2477, 3853 |
| BstSFI | CTRYAG | 3 cut(s) | 26, 1544, 2865 |
| BstSLI | GKGCMC | 2 cut(s) | 429, 2960 |
| BstUI | CGCG | 1 cut(s) | 1490 |
| BstV1I | GCAGC | 8 cut(s) | 12, 45, 298, 1558, 1629, 2918, 3737, 3817 |
| BstV2I | GAAGAC | 3 cut(s) | 181, 473, 1223 |
| BstX2I | RGATCY | 9 cut(s) | 155, 246, 292, 381, 1264, 2089, 2626, 2821, 3472 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 2204, 2953 |
| BstYI | RGATCY | 9 cut(s) | 155, 246, 292, 381, 1264, 2089, 2626, 2821, 3472 |
| Bsu36I | CCTNAGG | 2 cut(s) | 2889, 3702 |
| BsuRI | GGCC | 4 cut(s) | 75, 237, 1672, 2816 |
| BtgI | CCRYGG | 5 cut(s) | 76, 300, 2082, 2236, 3354 |
| BtsCI | GGATG | 8 cut(s) | 749, 873, 1330, 1936, 2428, 2550, 3132, 3911 |
| BtsIMutI | CAGTG | 4 cut(s) | 352, 919, 2053, 3458 |
| BveI | ACCTGC | 3 cut(s) | 562, 3415, 3722 |
| Cac8I | GCNNGC | 6 cut(s) | 23, 73, 116, 551, 1486, 3762 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3305 |
| CciI | TCATGA | 1 cut(s) | 1602 |
| CfoI | GCGC | 6 cut(s) | 21, 71, 216, 497, 1490, 3574 |
| Cfr10I | RCCGGY | 3 cut(s) | 71, 473, 607 |
| Cfr13I | GGNCC | 7 cut(s) | 208, 642, 787, 1432, 1671, 1821, 2681 |
| Cfr9I | CCCGGG | 1 cut(s) | 645 |
| CseI | GACGC | 2 cut(s) | 314, 489 |
| CsiI | ACCWGGT | 1 cut(s) | 2477 |
| CspAI | ACCGGT | 2 cut(s) | 473, 607 |
| DinI | GGCGCC | 2 cut(s) | 70, 496 |
| DraI | TTTAAA | 1 cut(s) | 2938 |
| DraIII | CACNNNGTG | 1 cut(s) | 1066 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 206 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 206 |
| EaeI | YGGCCR | 1 cut(s) | 73 |
| Eam1104I | CTCTTC | 5 cut(s) | 104, 827, 1968, 3170, 3285 |
| EarI | CTCTTC | 5 cut(s) | 104, 827, 1968, 3170, 3285 |
| EciI | GGCGGA | 1 cut(s) | 2102 |
| Ecl136II | GAGCTC | 2 cut(s) | 2544, 3199 |
| Eco130I | CCWWGG | 9 cut(s) | 300, 748, 863, 2082, 2236, 2630, 2817, 3136, 3354 |
| Eco24I | GRGCYC | 2 cut(s) | 2546, 3201 |
| Eco47I | GGWCC | 6 cut(s) | 208, 642, 787, 1432, 1821, 2681 |
| Eco47III | AGCGCT | 1 cut(s) | 215 |
| Eco53kI | GAGCTC | 2 cut(s) | 2544, 3199 |
| Eco57I | CTGAAG | 3 cut(s) | 1289, 2948, 3291 |
| Eco81I | CCTNAGG | 2 cut(s) | 2889, 3702 |
| Eco88I | CYCGRG | 2 cut(s) | 645, 1772 |
| Eco91I | GGTNACC | 1 cut(s) | 316 |
| EcoICRI | GAGCTC | 2 cut(s) | 2544, 3199 |
| EcoO109I | RGGNCCY | 1 cut(s) | 787 |
| EcoO65I | GGTNACC | 1 cut(s) | 316 |
| EcoRI | GAATTC | 2 cut(s) | 524, 3053 |
| EcoRII | CCWGG | 5 cut(s) | 387, 2005, 2131, 2477, 3853 |
| EcoT14I | CCWWGG | 9 cut(s) | 300, 748, 863, 2082, 2236, 2630, 2817, 3136, 3354 |
| EcoT22I | ATGCAT | 1 cut(s) | 2973 |
| EcoT38I | GRGCYC | 2 cut(s) | 2546, 3201 |
| EgeI | GGCGCC | 2 cut(s) | 70, 496 |
| EheI | GGCGCC | 2 cut(s) | 70, 496 |
| ErhI | CCWWGG | 9 cut(s) | 300, 748, 863, 2082, 2236, 2630, 2817, 3136, 3354 |
| Esp3I | CGTCTC | 1 cut(s) | 226 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 1349, 1381, 1892, 1924, 2156, 2188, 3792, 3824 |
| FaqI | GGGAC | 7 cut(s) | 215, 773, 1425, 1445, 1886, 2347, 2545 |
| FauI | CCCGC | 2 cut(s) | 30, 1402 |
| FbaI | TGATCA | 4 cut(s) | 1599, 1647, 2020, 2854 |
| FokI | GGATG | 7 cut(s) | 756, 880, 1337, 1943, 2435, 2537, 3119 |
| FriOI | GRGCYC | 2 cut(s) | 2546, 3201 |
| FspBI | CTAG | 4 cut(s) | 438, 1889, 2876, 2949 |
| GlaI | GCGC | 6 cut(s) | 20, 70, 215, 496, 1489, 3573 |
| GsaI | CCCAGC | 1 cut(s) | 3264 |
| GsuI | CTGGAG | 5 cut(s) | 140, 1340, 2807, 3527, 3837 |
| HaeII | RGCGCY | 4 cut(s) | 22, 72, 217, 498 |
| HaeIII | GGCC | 4 cut(s) | 75, 237, 1672, 2816 |
| HapII | CCGG | 8 cut(s) | 72, 234, 374, 410, 474, 608, 646, 837 |
| HgaI | GACGC | 2 cut(s) | 314, 489 |
| HhaI | GCGC | 6 cut(s) | 21, 71, 216, 497, 1490, 3574 |
| Hin1I | GRCGYC | 3 cut(s) | 69, 327, 495 |
| Hin6I | GCGC | 6 cut(s) | 19, 69, 214, 495, 1488, 3572 |
| HinP1I | GCGC | 6 cut(s) | 19, 69, 214, 495, 1488, 3572 |
| HincII | GTYRAC | 1 cut(s) | 268 |
| HindII | GTYRAC | 1 cut(s) | 268 |
| HindIII | AAGCTT | 5 cut(s) | 1782, 1795, 2033, 2387, 3390 |
| HpaII | CCGG | 8 cut(s) | 72, 234, 374, 410, 474, 608, 646, 837 |
| HphI | GGTGA | 8 cut(s) | 310, 472, 661, 2252, 2618, 3032, 3157, 3721 |
| Hpy99I | CGWCG | 7 cut(s) | 203, 257, 329, 332, 368, 455, 548 |
| HpyAV | CCTTC | 9 cut(s) | 757, 1338, 1675, 1859, 2020, 2321, 2516, 3463, 3577 |
| HpyCH4IV | ACGT | 4 cut(s) | 228, 327, 1065, 2586 |
| HpySE526I | ACGT | 4 cut(s) | 228, 327, 1065, 2586 |
| Hsp92I | GRCGYC | 3 cut(s) | 69, 327, 495 |
| HspAI | GCGC | 6 cut(s) | 19, 69, 214, 495, 1488, 3572 |
| KasI | GGCGCC | 2 cut(s) | 68, 494 |
| KflI | GGGWCCC | 1 cut(s) | 787 |
| KroI | GCCGGC | 1 cut(s) | 71 |
| KroNI | GCCGGC | 1 cut(s) | 73 |
| Ksp22I | TGATCA | 4 cut(s) | 1599, 1647, 2020, 2854 |
| LguI | GCTCTTC | 1 cut(s) | 104 |
| Lsp1109I | GCAGC | 8 cut(s) | 12, 45, 298, 1558, 1629, 2918, 3737, 3817 |
| LweI | GCATC | 7 cut(s) | 351, 734, 1315, 1600, 2980, 3469, 3896 |
| MabI | ACCWGGT | 1 cut(s) | 2477 |
| MaeI | CTAG | 4 cut(s) | 438, 1889, 2876, 2949 |
| MaeII | ACGT | 4 cut(s) | 228, 327, 1065, 2586 |
| MfeI | CAATTG | 2 cut(s) | 1941, 3360 |
| MflI | RGATCY | 9 cut(s) | 155, 246, 292, 381, 1264, 2089, 2626, 2821, 3472 |
| MhlI | GDGCHC | 6 cut(s) | 126, 429, 2371, 2546, 2960, 3201 |
| Mly113I | GGCGCC | 2 cut(s) | 69, 495 |
| MlyI | GAGTC | 5 cut(s) | 916, 1486, 2329, 2758, 2798 |
| MmeI | TCCRAC | 4 cut(s) | 160, 3063, 3078, 3226 |
| Mph1103I | ATGCAT | 1 cut(s) | 2973 |
| MroNI | GCCGGC | 1 cut(s) | 71 |
| MroXI | GAANNNNTTC | 4 cut(s) | 1230, 1907, 3021, 3212 |
| MslI | CAYNNNNRTG | 6 cut(s) | 347, 1440, 2238, 2270, 2364, 2951 |
| MspA1I | CMGCKG | 3 cut(s) | 25, 118, 3728 |
| MspI | CCGG | 8 cut(s) | 72, 234, 374, 410, 474, 608, 646, 837 |
| MspR9I | CCNGG | 8 cut(s) | 234, 389, 646, 647, 2007, 2133, 2479, 3855 |
| MunI | CAATTG | 2 cut(s) | 1941, 3360 |
| Mva1269I | GAATGC | 2 cut(s) | 2221, 3574 |
| MvaI | CCWGG | 5 cut(s) | 389, 2007, 2133, 2479, 3855 |
| MvnI | CGCG | 1 cut(s) | 1490 |
| NaeI | GCCGGC | 1 cut(s) | 73 |
| NarI | GGCGCC | 2 cut(s) | 69, 495 |
| NciI | CCSGG | 3 cut(s) | 234, 646, 647 |
| NcoI | CCATGG | 4 cut(s) | 300, 2082, 2236, 3354 |
| NgoMIV | GCCGGC | 1 cut(s) | 71 |
| NmuCI | GTSAC | 6 cut(s) | 316, 649, 914, 988, 2048, 3456 |
| NsiI | ATGCAT | 1 cut(s) | 2973 |
| NspI | RCATGY | 1 cut(s) | 2971 |
| OliI | CACNNNNGTG | 1 cut(s) | 2238 |
| PagI | TCATGA | 1 cut(s) | 1602 |
| PaqCI | CACCTGC | 1 cut(s) | 562 |
| PciSI | GCTCTTC | 1 cut(s) | 104 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 198, 540 |
| PctI | GAATGC | 2 cut(s) | 2221, 3574 |
| PdiI | GCCGGC | 1 cut(s) | 73 |
| PdmI | GAANNNNTTC | 4 cut(s) | 1230, 1907, 3021, 3212 |
| PfeI | GAWTC | 8 cut(s) | 259, 1121, 1147, 1385, 1534, 1885, 3129, 3845 |
| PinAI | ACCGGT | 2 cut(s) | 473, 607 |
| Ple19I | CGATCG | 1 cut(s) | 90 |
| PleI | GAGTC | 5 cut(s) | 915, 1486, 2329, 2758, 2797 |
| PluTI | GGCGCC | 2 cut(s) | 72, 498 |
| PpsI | GAGTC | 5 cut(s) | 915, 1486, 2329, 2758, 2797 |
| PpuMI | RGGWCCY | 1 cut(s) | 787 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1496 |
| PshBI | ATTAAT | 1 cut(s) | 2304 |
| PsiI | TTATAA | 4 cut(s) | 1712, 2562, 2775, 3876 |
| Psp124BI | GAGCTC | 2 cut(s) | 2546, 3201 |
| Psp1406I | AACGTT | 1 cut(s) | 2586 |
| Psp5II | RGGWCCY | 1 cut(s) | 787 |
| Psp6I | CCWGG | 5 cut(s) | 387, 2005, 2131, 2477, 3853 |
| PspEI | GGTNACC | 1 cut(s) | 316 |
| PspFI | CCCAGC | 1 cut(s) | 3260 |
| PspGI | CCWGG | 5 cut(s) | 387, 2005, 2131, 2477, 3853 |
| PspPI | GGNCC | 7 cut(s) | 208, 642, 787, 1432, 1671, 1821, 2681 |
| PspPPI | RGGWCCY | 1 cut(s) | 787 |
| PstI | CTGCAG | 2 cut(s) | 30, 1548 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3305 |
| PsuI | RGATCY | 9 cut(s) | 155, 246, 292, 381, 1264, 2089, 2626, 2821, 3472 |
| PvuI | CGATCG | 1 cut(s) | 90 |
| PvuII | CAGCTG | 1 cut(s) | 118 |
| RseI | CAYNNNNRTG | 6 cut(s) | 347, 1440, 2238, 2270, 2364, 2951 |
| SacI | GAGCTC | 2 cut(s) | 2546, 3201 |
| SapI | GCTCTTC | 1 cut(s) | 104 |
| Sau96I | GGNCC | 7 cut(s) | 208, 642, 787, 1432, 1671, 1821, 2681 |
| ScaI | AGTACT | 3 cut(s) | 1855, 2260, 2863 |
| SchI | GAGTC | 5 cut(s) | 916, 1486, 2329, 2758, 2798 |
| ScrFI | CCNGG | 8 cut(s) | 234, 389, 646, 647, 2007, 2133, 2479, 3855 |
| SduI | GDGCHC | 6 cut(s) | 126, 429, 2371, 2546, 2960, 3201 |
| SexAI | ACCWGGT | 1 cut(s) | 2477 |
| SfaNI | GCATC | 7 cut(s) | 351, 734, 1315, 1600, 2980, 3469, 3896 |
| SfcI | CTRYAG | 3 cut(s) | 26, 1544, 2865 |
| SfoI | GGCGCC | 2 cut(s) | 70, 496 |
| SgrAI | CRCCGGYG | 1 cut(s) | 473 |
| SinI | GGWCC | 6 cut(s) | 208, 642, 787, 1432, 1821, 2681 |
| SmaI | CCCGGG | 1 cut(s) | 647 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 347, 1440, 2238, 2270, 2364, 2951 |
| SmlI | CTYRAG | 9 cut(s) | 311, 1052, 1494, 1798, 2766, 2799, 3026, 3092, 3194 |
| SmoI | CTYRAG | 9 cut(s) | 311, 1052, 1494, 1798, 2766, 2799, 3026, 3092, 3194 |
| SpeI | ACTAGT | 1 cut(s) | 437 |
| SspDI | GGCGCC | 2 cut(s) | 68, 494 |
| SspI | AATATT | 1 cut(s) | 1525 |
| SspMI | CTAG | 4 cut(s) | 438, 1889, 2876, 2949 |
| SstI | GAGCTC | 2 cut(s) | 2546, 3201 |
| StyD4I | CCNGG | 8 cut(s) | 232, 387, 644, 645, 2005, 2131, 2477, 3853 |
| StyI | CCWWGG | 9 cut(s) | 300, 748, 863, 2082, 2236, 2630, 2817, 3136, 3354 |
| TaiI | ACGT | 4 cut(s) | 231, 330, 1068, 2589 |
| TaqII | GACCGA | 1 cut(s) | 1622 |
| TatI | WGTACW | 6 cut(s) | 351, 564, 1853, 2043, 2258, 2861 |
| TauI | GCSGC | 4 cut(s) | 56, 275, 556, 3731 |
| TfiI | GAWTC | 8 cut(s) | 259, 1121, 1147, 1385, 1534, 1885, 3129, 3845 |
| TscAI | CASTG | 4 cut(s) | 352, 919, 2053, 3465 |
| TseFI | GTSAC | 6 cut(s) | 316, 649, 914, 988, 2048, 3456 |
| TseI | GCWGC | 8 cut(s) | 25, 33, 286, 1546, 1642, 2906, 3725, 3830 |
| Tsp45I | GTSAC | 6 cut(s) | 316, 649, 914, 988, 2048, 3456 |
| TspGWI | ACGGA | 6 cut(s) | 208, 285, 381, 1307, 2744, 3730 |
| TspMI | CCCGGG | 1 cut(s) | 645 |
| TspRI | CASTG | 4 cut(s) | 352, 919, 2053, 3465 |
| VpaK11BI | GGWCC | 6 cut(s) | 208, 642, 787, 1432, 1821, 2681 |
| VspI | ATTAAT | 1 cut(s) | 2304 |
| XapI | RAATTY | 6 cut(s) | 524, 674, 2780, 2871, 3021, 3053 |
| XbaI | TCTAGA | 1 cut(s) | 1888 |
| XceI | RCATGY | 1 cut(s) | 2971 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 2628, 2757, 3459, 3679 |
| XmaI | CCCGGG | 1 cut(s) | 645 |
| XmnI | GAANNNNTTC | 4 cut(s) | 1230, 1907, 3021, 3212 |
| XspI | CTAG | 4 cut(s) | 438, 1889, 2876, 2949 |
| ZraI | GACGTC | 1 cut(s) | 328 |
| ZrmI | AGTACT | 3 cut(s) | 1855, 2260, 2863 |
| Zsp2I | ATGCAT | 1 cut(s) | 2973 |