RchiOBHm_Chr1g0380651

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001532
Physical Location & Seq
Reverse (-)
66242511 .. 66244273
1763 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60391

Sequence Viewer

Length: 807 bp
ATGATAGAATGGTGCCCATGGATGATGGATCCTTGGTACAGAGGCTTTACCGGAACTACTGAGAAAACTGTTACAGATTCTGGCGTGTGCTACAGTACTTGTGGGATCATAAAGAAAGAAAATGAGTCAACACTCAGGATTTCGGAGCTGCCAATTGGTACTTGCATTAAAGATTATTTGGAATTTCTTGACTCTATCTCGGAAGTGAATGGAGAGGCCAAGGATCATTGGATTAAGGATTACGAAGATTTTTCCTACATAGATGTTGATATTCTCATCCACTTGTCTGATACTGTGAAGAGCTTGACAGAAGCCAGTGAAGAGGATCTGTGTAAGAAGTTTAAACTGACAAGTAGCAGAAGCATAAGTGAGATGCACCTGTTTGATCCAACAGGCGAGATTAAGAAATATGACACTCCAGAACAAATTCTTGAGGAATTTTTTCATCTAAGACTCCAATTCTATGAGAAAAGAAAGACAGTACTATTGCTTATCCTTAGATCGGAGTTGTCAAATTTGGAAAACAAGGTTGCACTTATGCGTGGTGTCTTTGATGGAGGAGTGGTCGACTTCAATAAACTTAGCACTGATCCATACCTAAAAAGCATGCCAATTACTACGTTTGTCTACAGAGACAGTTATCAAGAAAAGTTGCAAGAATTATTGGCTGATATAGATACGATCAAACTGAAGATTAAAAAGTTGGAAGGTTCAACTCCGAGGACATTGTGGTTGGAGGAGCTTGCTGCTCTTGAACAGGAACTTAATAAGCTGGAGAAGCAGAAGCTGTTGGTGGAACTAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 map01524
Pfam Domains
Protein Families
DNA_gyraseB DNA_topoisoIV HATPase_c TOPRIM_C Toprim

Protein Analysis

268

Amino Acids

31.11

Weight (kDa)

4.88

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_topoisoIV PF00521 8 - 182 1.1e-23 DNA gyrase/topoisomerase IV, subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 12
AccI GTMKAC 2 cut(s) 567, 627
AclWI GGATC 7 cut(s) 23, 36, 113, 231, 333, 380, 584
AcsI RAATTY 4 cut(s) 182, 426, 437, 514
AcuI CTGAAG 1 cut(s) 710
AfaI GTAC 4 cut(s) 38, 97, 160, 483
AfiI CCNNNNNNNGG 1 cut(s) 502
AgsI TTSAA 3 cut(s) 574, 714, 755
AluBI AGCT 5 cut(s) 148, 303, 742, 772, 787
AluI AGCT 5 cut(s) 148, 303, 742, 772, 787
Alw26I GTCTC 1 cut(s) 627
AlwI GGATC 7 cut(s) 23, 36, 113, 231, 333, 380, 584
AlwNI CAGNNNCTG 2 cut(s) 80, 787
AoxI GGCC 1 cut(s) 216
ApeKI GCWGC 2 cut(s) 148, 746
ApoI RAATTY 4 cut(s) 182, 426, 437, 514
Asp700I GAANNNNTTC 2 cut(s) 426, 441
BaeGI GKGCMC 1 cut(s) 17
BamHI GGATCC 1 cut(s) 28
BanI GGYRCC 1 cut(s) 12
BbvI GCAGC 2 cut(s) 135, 733
BccI CCATC 2 cut(s) 19, 548
BcoDI GTCTC 1 cut(s) 627
BfaI CTAG 1 cut(s) 800
BfmI CTRYAG 2 cut(s) 91, 628
BisI GCNGC 2 cut(s) 149, 747
BlsI GCNGC 2 cut(s) 150, 748
BmcAI AGTACT 2 cut(s) 97, 483
BmiI GGNNCC 2 cut(s) 14, 30
BmsI GCATC 1 cut(s) 363
BpmI CTGGAG 2 cut(s) 402, 794
BpuEI CTTGAG 1 cut(s) 452
BsaJI CCNNGG 4 cut(s) 17, 32, 219, 719
BsaWI WCCGGW 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 1 cut(s) 502
Bse1I ACTGG 1 cut(s) 315
BseDI CCNNGG 4 cut(s) 17, 32, 219, 719
BseGI GGATG 2 cut(s) 27, 276
BseLI CCNNNNNNNGG 1 cut(s) 502
BseMII CTCAG 2 cut(s) 51, 148
BseNI ACTGG 1 cut(s) 315
BseRI GAGGAG 2 cut(s) 573, 752
BseSI GKGCMC 1 cut(s) 17
BseXI GCAGC 2 cut(s) 135, 733
BshFI GGCC 1 cut(s) 218
BshNI GGYRCC 1 cut(s) 12
BsiSI CCGG 1 cut(s) 51
BslI CCNNNNNNNGG 1 cut(s) 502
BsmAI GTCTC 1 cut(s) 627
BsnI GGCC 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 8 cut(s) 28, 105, 223, 325, 385, 500, 589, 681
Bsp19I CCATGG 1 cut(s) 17
BspANI GGCC 1 cut(s) 218
BspCNI CTCAG 2 cut(s) 52, 147
BspLI GGNNCC 2 cut(s) 14, 30
BspPI GGATC 7 cut(s) 23, 36, 113, 231, 333, 380, 584
BspQI GCTCTTC 1 cut(s) 293
BspT107I GGYRCC 1 cut(s) 12
BsrI ACTGG 1 cut(s) 315
BssECI CCNNGG 4 cut(s) 17, 32, 219, 719
BssMI GATC 8 cut(s) 28, 105, 223, 325, 385, 500, 589, 681
BssT1I CCWWGG 3 cut(s) 17, 32, 219
Bst4CI ACNGT 5 cut(s) 70, 95, 295, 481, 638
Bst6I CTCTTC 2 cut(s) 293, 315
BstC8I GCNNGC 2 cut(s) 608, 744
BstDEI CTNAG 5 cut(s) 60, 134, 449, 497, 581
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 2 cut(s) 27, 276
BstKTI GATC 8 cut(s) 31, 108, 226, 328, 388, 503, 592, 684
BstMAI GTCTC 1 cut(s) 627
BstMBI GATC 8 cut(s) 28, 105, 223, 325, 385, 500, 589, 681
BstMWI GCNNNNNNNGC 1 cut(s) 778
BstNSI RCATGY 1 cut(s) 610
BstSFI CTRYAG 2 cut(s) 91, 628
BstSLI GKGCMC 1 cut(s) 17
BstV1I GCAGC 2 cut(s) 135, 733
BstX2I RGATCY 2 cut(s) 28, 325
BstYI RGATCY 2 cut(s) 28, 325
BsuRI GGCC 1 cut(s) 218
BtgI CCRYGG 1 cut(s) 17
BtsCI GGATG 2 cut(s) 27, 276
BtsIMutI CAGTG 2 cut(s) 322, 585
Cac8I GCNNGC 2 cut(s) 608, 744
CaiI CAGNNNCTG 2 cut(s) 80, 787
Csp6I GTAC 4 cut(s) 37, 96, 159, 482
CviAII CATG 2 cut(s) 18, 607
CviJI RGCY 9 cut(s) 45, 148, 218, 303, 314, 668, 742, 772, 787
CviKI_1 RGCY 9 cut(s) 45, 148, 218, 303, 314, 668, 742, 772, 787
CviQI GTAC 4 cut(s) 37, 96, 159, 482
DdeI CTNAG 5 cut(s) 60, 134, 449, 497, 581
DpnI GATC 8 cut(s) 30, 107, 225, 327, 387, 502, 591, 683
DpnII GATC 8 cut(s) 28, 105, 223, 325, 385, 500, 589, 681
DraI TTTAAA 1 cut(s) 343
Eam1104I CTCTTC 2 cut(s) 293, 315
EarI CTCTTC 2 cut(s) 293, 315
Eco130I CCWWGG 3 cut(s) 17, 32, 219
Eco57I CTGAAG 1 cut(s) 710
EcoT14I CCWWGG 3 cut(s) 17, 32, 219
ErhI CCWWGG 3 cut(s) 17, 32, 219
FaeI CATG 2 cut(s) 21, 610
FatI CATG 2 cut(s) 17, 606
FblI GTMKAC 2 cut(s) 567, 627
Fnu4HI GCNGC 2 cut(s) 149, 747
FokI GGATG 2 cut(s) 34, 263
Fsp4HI GCNGC 2 cut(s) 149, 747
FspBI CTAG 1 cut(s) 800
GluI GCNGC 2 cut(s) 149, 747
GsuI CTGGAG 2 cut(s) 402, 794
HaeIII GGCC 1 cut(s) 218
HapII CCGG 1 cut(s) 51
Hin1II CATG 2 cut(s) 21, 610
HincII GTYRAC 2 cut(s) 129, 568
HindII GTYRAC 2 cut(s) 129, 568
HinfI GANTC 4 cut(s) 77, 125, 191, 453
HpaII CCGG 1 cut(s) 51
Hpy166II GTNNAC 3 cut(s) 129, 568, 628
Hpy188I TCNGA 5 cut(s) 145, 202, 289, 505, 720
Hpy188III TCNNGA 6 cut(s) 136, 188, 419, 431, 644, 752
Hpy8I GTNNAC 3 cut(s) 129, 568, 628
HpyAV CCTTC 1 cut(s) 701
HpyCH4III ACNGT 5 cut(s) 70, 95, 295, 481, 638
HpyCH4IV ACGT 1 cut(s) 620
HpyCH4V TGCA 4 cut(s) 165, 376, 533, 655
HpyF10VI GCNNNNNNNGC 1 cut(s) 778
HpyF3I CTNAG 5 cut(s) 60, 134, 449, 497, 581
HpySE526I ACGT 1 cut(s) 620
Hsp92II CATG 2 cut(s) 21, 610
Kzo9I GATC 8 cut(s) 28, 105, 223, 325, 385, 500, 589, 681
LguI GCTCTTC 1 cut(s) 293
LmnI GCTCC 2 cut(s) 145, 739
LpnPI CCDG 9 cut(s) 64, 66, 121, 328, 378, 392, 432, 743, 758
Lsp1109I GCAGC 2 cut(s) 135, 733
LweI GCATC 1 cut(s) 363
MaeI CTAG 1 cut(s) 800
MaeII ACGT 1 cut(s) 620
MaeIII GTNAC 1 cut(s) 70
MalI GATC 8 cut(s) 30, 107, 225, 327, 387, 502, 591, 683
MboI GATC 8 cut(s) 28, 105, 223, 325, 385, 500, 589, 681
MboII GAAGA 4 cut(s) 257, 310, 332, 703
MfeI CAATTG 1 cut(s) 153
MflI RGATCY 2 cut(s) 28, 325
MhlI GDGCHC 1 cut(s) 17
MluCI AATT 8 cut(s) 153, 182, 426, 437, 458, 514, 612, 659
MlyI GAGTC 3 cut(s) 134, 185, 447
MmeI TCCRAC 3 cut(s) 413, 684, 714
MnlI CCTC 7 cut(s) 35, 208, 316, 427, 551, 714, 730
MroXI GAANNNNTTC 2 cut(s) 426, 441
MseI TTAA 6 cut(s) 168, 234, 342, 402, 696, 765
MspI CCGG 1 cut(s) 51
MssI GTTTAAAC 1 cut(s) 343
MunI CAATTG 1 cut(s) 153
MwoI GCNNNNNNNGC 1 cut(s) 778
NcoI CCATGG 1 cut(s) 17
NdeII GATC 8 cut(s) 28, 105, 223, 325, 385, 500, 589, 681
NlaIII CATG 2 cut(s) 21, 610
NlaIV GGNNCC 2 cut(s) 14, 30
NspI RCATGY 1 cut(s) 610
PaeI GCATGC 1 cut(s) 610
PciSI GCTCTTC 1 cut(s) 293
PdmI GAANNNNTTC 2 cut(s) 426, 441
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 2 cut(s) 150, 748
PleI GAGTC 3 cut(s) 133, 185, 447
PmeI GTTTAAAC 1 cut(s) 343
PpsI GAGTC 3 cut(s) 133, 185, 447
PspN4I GGNNCC 2 cut(s) 14, 30
PstNI CAGNNNCTG 2 cut(s) 80, 787
PsuI RGATCY 2 cut(s) 28, 325
RsaI GTAC 4 cut(s) 38, 97, 160, 483
RsaNI GTAC 4 cut(s) 37, 96, 159, 482
SalI GTCGAC 1 cut(s) 566
SapI GCTCTTC 1 cut(s) 293
SaqAI TTAA 6 cut(s) 168, 234, 342, 402, 696, 765
SatI GCNGC 2 cut(s) 149, 747
Sau3AI GATC 8 cut(s) 28, 105, 223, 325, 385, 500, 589, 681
ScaI AGTACT 2 cut(s) 97, 483
SchI GAGTC 3 cut(s) 134, 185, 447
SduI GDGCHC 1 cut(s) 17
SfaNI GCATC 1 cut(s) 363
SfcI CTRYAG 2 cut(s) 91, 628
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
SphI GCATGC 1 cut(s) 610
Sse9I AATT 8 cut(s) 153, 182, 426, 437, 458, 514, 612, 659
SspMI CTAG 1 cut(s) 800
StyI CCWWGG 3 cut(s) 17, 32, 219
TaaI ACNGT 5 cut(s) 70, 95, 295, 481, 638
TaiI ACGT 1 cut(s) 623
TaqI TCGA 1 cut(s) 567
TasI AATT 8 cut(s) 153, 182, 426, 437, 458, 514, 612, 659
TatI WGTACW 2 cut(s) 95, 481
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 6 cut(s) 168, 234, 342, 402, 696, 765
Tru9I TTAA 6 cut(s) 168, 234, 342, 402, 696, 765
TscAI CASTG 2 cut(s) 322, 592
TseI GCWGC 2 cut(s) 148, 746
TspDTI ATGAA 1 cut(s) 434
TspRI CASTG 2 cut(s) 322, 592
XapI RAATTY 4 cut(s) 182, 426, 437, 514
XceI RCATGY 1 cut(s) 610
XmiI GTMKAC 2 cut(s) 567, 627
XmnI GAANNNNTTC 2 cut(s) 426, 441
XspI CTAG 1 cut(s) 800
ZrmI AGTACT 2 cut(s) 97, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.