Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| HATPase_c | PF02518 | 80 - 174 | 3.2e-07 | Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase |
| DNA_gyraseB | PF00204 | 270 - 426 | 5.6e-25 | DNA gyrase B |
| Toprim | PF01751 | 456 - 555 | 1.1e-08 | Toprim domain |
| TOPRIM_C | PF16898 | 571 - 698 | 3.7e-51 | C-terminal associated domain of TOPRIM |
| DNA_topoisoIV | PF00521 | 701 - 1160 | 8.4e-118 | DNA gyrase/topoisomerase IV, subunit A |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 1715, 2565, 2778 |
| AarI | CACCTGC | 1 cut(s) | 565 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 209 |
| AatII | GACGTC | 1 cut(s) | 333 |
| Acc36I | ACCTGC | 3 cut(s) | 565, 3418, 3725 |
| Acc65I | GGTACC | 1 cut(s) | 2620 |
| AccB1I | GGYRCC | 5 cut(s) | 55, 71, 429, 497, 2620 |
| AccII | CGCG | 1 cut(s) | 53 |
| AcoI | YGGCCR | 2 cut(s) | 76, 4059 |
| AcsI | RAATTY | 6 cut(s) | 527, 677, 2783, 2874, 3024, 3056 |
| AcuI | CTGAAG | 5 cut(s) | 1292, 2951, 3294, 3894, 4095 |
| AcyI | GRCGYC | 4 cut(s) | 56, 72, 330, 498 |
| AdeI | CACNNNGTG | 1 cut(s) | 1069 |
| AfeI | AGCGCT | 1 cut(s) | 218 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 1135, 1460, 3100, 3145, 3384, 4057 |
| AgeI | ACCGGT | 2 cut(s) | 476, 610 |
| AhlI | ACTAGT | 1 cut(s) | 440 |
| AjnI | CCWGG | 4 cut(s) | 390, 2008, 2134, 2480 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 2813, 2845, 3068, 3100, 3297, 3329 |
| AleI | CACNNNNGTG | 1 cut(s) | 2241 |
| Alw21I | GWGCWC | 5 cut(s) | 129, 777, 2374, 2549, 3204 |
| Alw26I | GTCTC | 5 cut(s) | 229, 917, 2568, 2720, 4098 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3308 |
| Ama87I | CYCGRG | 2 cut(s) | 648, 1775 |
| Aor51HI | AGCGCT | 1 cut(s) | 218 |
| AoxI | GGCC | 7 cut(s) | 76, 238, 1673, 2817, 3918, 4059, 4114 |
| ApoI | RAATTY | 6 cut(s) | 527, 677, 2783, 2874, 3024, 3056 |
| AseI | ATTAAT | 1 cut(s) | 2307 |
| AsiGI | ACCGGT | 2 cut(s) | 476, 610 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 1233, 1910, 3024, 3215 |
| Asp718I | GGTACC | 1 cut(s) | 2620 |
| AspLEI | GCGC | 6 cut(s) | 21, 58, 74, 219, 500, 3577 |
| AspS9I | GGNCC | 8 cut(s) | 211, 645, 790, 1435, 1674, 1824, 2684, 4073 |
| AsuC2I | CCSGG | 4 cut(s) | 237, 649, 650, 4238 |
| AsuHPI | GGTGA | 9 cut(s) | 280, 313, 380, 664, 2255, 2621, 3035, 3160, 3724 |
| AvaI | CYCGRG | 2 cut(s) | 648, 1775 |
| AvaII | GGWCC | 7 cut(s) | 211, 645, 790, 1435, 1824, 2684, 4073 |
| AxyI | CCTNAGG | 2 cut(s) | 2892, 3705 |
| BaeGI | GKGCMC | 2 cut(s) | 432, 2963 |
| BamHI | GGATCC | 3 cut(s) | 158, 2629, 2824 |
| BanI | GGYRCC | 5 cut(s) | 55, 71, 429, 497, 2620 |
| BanII | GRGCYC | 3 cut(s) | 777, 2549, 3204 |
| BauI | CACGAG | 1 cut(s) | 1240 |
| BbsI | GAAGAC | 3 cut(s) | 184, 476, 1226 |
| Bbv12I | GWGCWC | 5 cut(s) | 129, 777, 2374, 2549, 3204 |
| BceAI | ACGGC | 7 cut(s) | 43, 85, 97, 379, 505, 511, 565 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 73, 107 |
| BciT130I | CCWGG | 4 cut(s) | 392, 2010, 2136, 2482 |
| BclI | TGATCA | 4 cut(s) | 1602, 1650, 2023, 2857 |
| BcnI | CCSGG | 4 cut(s) | 237, 649, 650, 4238 |
| BcoDI | GTCTC | 5 cut(s) | 229, 917, 2568, 2720, 4098 |
| BcuI | ACTAGT | 1 cut(s) | 440 |
| BfaI | CTAG | 7 cut(s) | 441, 1892, 2879, 2952, 3833, 3924, 4308 |
| BfmI | CTRYAG | 4 cut(s) | 26, 1547, 2868, 3984 |
| BfoI | RGCGCY | 5 cut(s) | 22, 59, 75, 220, 501 |
| BfuAI | ACCTGC | 3 cut(s) | 565, 3418, 3725 |
| BglII | AGATCT | 2 cut(s) | 249, 1267 |
| BlpI | GCTNAGC | 1 cut(s) | 4044 |
| BmcAI | AGTACT | 2 cut(s) | 1858, 2866 |
| Bme1390I | CCNGG | 8 cut(s) | 237, 392, 649, 650, 2010, 2136, 2482, 4238 |
| Bme18I | GGWCC | 7 cut(s) | 211, 645, 790, 1435, 1824, 2684, 4073 |
| BmeT110I | CYCGRG | 2 cut(s) | 648, 1775 |
| BmgT120I | GGNCC | 8 cut(s) | 211, 645, 790, 1435, 1674, 1824, 2684, 4073 |
| BmrFI | CCNGG | 8 cut(s) | 237, 392, 649, 650, 2010, 2136, 2482, 4238 |
| BmrI | ACTGGG | 1 cut(s) | 2194 |
| BmsI | GCATC | 7 cut(s) | 354, 737, 1318, 1603, 2983, 3472, 4357 |
| BmuI | ACTGGG | 1 cut(s) | 2194 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1499 |
| BpiI | GAAGAC | 3 cut(s) | 184, 476, 1226 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 2783, 2815, 4186, 4218 |
| BpmI | CTGGAG | 7 cut(s) | 143, 1343, 2810, 3530, 3843, 4038, 4272 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1395, 3606 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 4044 |
| BpuEI | CTTGAG | 9 cut(s) | 299, 1040, 1482, 1822, 2754, 2787, 3050, 3116, 3218 |
| BpuMI | CCSGG | 4 cut(s) | 237, 649, 650, 4238 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1617 |
| BsaHI | GRCGYC | 4 cut(s) | 56, 72, 330, 498 |
| BsaWI | WCCGGW | 3 cut(s) | 376, 476, 610 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 363, 393, 1613, 1643, 4270, 4300 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 1135, 1460, 3100, 3145, 3384, 4057 |
| Bse118I | RCCGGY | 3 cut(s) | 74, 476, 610 |
| Bse21I | CCTNAGG | 2 cut(s) | 2892, 3705 |
| Bse3DI | GCAATG | 3 cut(s) | 954, 1413, 3690 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1617 |
| BseBI | CCWGG | 4 cut(s) | 392, 2010, 2136, 2482 |
| BseGI | GGATG | 9 cut(s) | 752, 876, 1333, 1939, 2431, 2553, 3135, 3808, 4372 |
| BseJI | GATNNNNATC | 1 cut(s) | 1617 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 1135, 1460, 3100, 3145, 3384, 4057 |
| BseMI | GCAATG | 3 cut(s) | 954, 1413, 3690 |
| BseRI | GAGGAG | 3 cut(s) | 410, 2909, 3047 |
| BseSI | GKGCMC | 2 cut(s) | 432, 2963 |
| BseYI | CCCAGC | 1 cut(s) | 3263 |
| BsgI | GTGCAG | 1 cut(s) | 3747 |
| Bsh1236I | CGCG | 1 cut(s) | 53 |
| Bsh1285I | CGRYCG | 1 cut(s) | 93 |
| BshFI | GGCC | 7 cut(s) | 78, 240, 1675, 2819, 3920, 4061, 4116 |
| BshNI | GGYRCC | 5 cut(s) | 55, 71, 429, 497, 2620 |
| BshTI | ACCGGT | 2 cut(s) | 476, 610 |
| BsiEI | CGRYCG | 1 cut(s) | 93 |
| BsiHKAI | GWGCWC | 5 cut(s) | 129, 777, 2374, 2549, 3204 |
| BsiHKCI | CYCGRG | 2 cut(s) | 648, 1775 |
| BsiSI | CCGG | 8 cut(s) | 75, 237, 377, 413, 477, 611, 649, 4237 |
| BslFI | GGGAC | 7 cut(s) | 218, 776, 1428, 1448, 1889, 2350, 2548 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 1135, 1460, 3100, 3145, 3384, 4057 |
| BsmAI | GTCTC | 5 cut(s) | 229, 917, 2568, 2720, 4098 |
| BsmBI | CGTCTC | 1 cut(s) | 229 |
| BsmFI | GGGAC | 7 cut(s) | 218, 776, 1428, 1448, 1889, 2350, 2548 |
| BsmI | GAATGC | 2 cut(s) | 2224, 3577 |
| BsnI | GGCC | 7 cut(s) | 78, 240, 1675, 2819, 3920, 4061, 4116 |
| BsoBI | CYCGRG | 2 cut(s) | 648, 1775 |
| Bsp1286I | GDGCHC | 7 cut(s) | 129, 432, 777, 2374, 2549, 2963, 3204 |
| Bsp1407I | TGTACA | 1 cut(s) | 354 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 4044 |
| Bsp19I | CCATGG | 4 cut(s) | 303, 2085, 2239, 3357 |
| BspANI | GGCC | 7 cut(s) | 78, 240, 1675, 2819, 3920, 4061, 4116 |
| BspFNI | CGCG | 1 cut(s) | 53 |
| BspHI | TCATGA | 1 cut(s) | 1605 |
| BspMAI | CTGCAG | 3 cut(s) | 30, 1551, 3988 |
| BspMI | ACCTGC | 3 cut(s) | 565, 3418, 3725 |
| BspQI | GCTCTTC | 2 cut(s) | 107, 4297 |
| BspT107I | GGYRCC | 5 cut(s) | 55, 71, 429, 497, 2620 |
| BsrDI | GCAATG | 3 cut(s) | 954, 1413, 3690 |
| BsrFI | RCCGGY | 3 cut(s) | 74, 476, 610 |
| BsrGI | TGTACA | 1 cut(s) | 354 |
| BssAI | RCCGGY | 3 cut(s) | 74, 476, 610 |
| BssNI | GRCGYC | 4 cut(s) | 56, 72, 330, 498 |
| BssSI | CACGAG | 1 cut(s) | 1240 |
| BssT1I | CCWWGG | 9 cut(s) | 303, 751, 866, 2085, 2239, 2633, 2820, 3139, 3357 |
| Bst2BI | CACGAG | 1 cut(s) | 1240 |
| Bst2UI | CCWGG | 4 cut(s) | 392, 2010, 2136, 2482 |
| Bst6I | CTCTTC | 8 cut(s) | 107, 1971, 3173, 3288, 4061, 4211, 4266, 4297 |
| BstACI | GRCGYC | 4 cut(s) | 56, 72, 330, 498 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 133, 2195 |
| BstAUI | TGTACA | 1 cut(s) | 354 |
| BstC8I | GCNNGC | 7 cut(s) | 23, 76, 119, 554, 3765, 4014, 4146 |
| BstDSI | CCRYGG | 6 cut(s) | 79, 303, 2085, 2239, 3357, 4053 |
| BstEII | GGTNACC | 1 cut(s) | 319 |
| BstF5I | GGATG | 9 cut(s) | 752, 876, 1333, 1939, 2431, 2553, 3135, 3808, 4372 |
| BstFNI | CGCG | 1 cut(s) | 53 |
| BstH2I | RGCGCY | 5 cut(s) | 22, 59, 75, 220, 501 |
| BstHHI | GCGC | 6 cut(s) | 21, 58, 74, 219, 500, 3577 |
| BstMAI | GTCTC | 5 cut(s) | 229, 917, 2568, 2720, 4098 |
| BstMCI | CGRYCG | 1 cut(s) | 93 |
| BstNI | CCWGG | 4 cut(s) | 392, 2010, 2136, 2482 |
| BstNSI | RCATGY | 1 cut(s) | 2974 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1499 |
| BstPI | GGTNACC | 1 cut(s) | 319 |
| BstSCI | CCNGG | 8 cut(s) | 235, 390, 647, 648, 2008, 2134, 2480, 4236 |
| BstSFI | CTRYAG | 4 cut(s) | 26, 1547, 2868, 3984 |
| BstSLI | GKGCMC | 2 cut(s) | 432, 2963 |
| BstUI | CGCG | 1 cut(s) | 53 |
| BstV2I | GAAGAC | 3 cut(s) | 184, 476, 1226 |
| BstX2I | RGATCY | 8 cut(s) | 158, 249, 295, 1267, 2092, 2629, 2824, 3475 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 2207, 2956 |
| BstYI | RGATCY | 8 cut(s) | 158, 249, 295, 1267, 2092, 2629, 2824, 3475 |
| Bsu36I | CCTNAGG | 2 cut(s) | 2892, 3705 |
| BsuRI | GGCC | 7 cut(s) | 78, 240, 1675, 2819, 3920, 4061, 4116 |
| BtgI | CCRYGG | 6 cut(s) | 79, 303, 2085, 2239, 3357, 4053 |
| BtgZI | GCGATG | 1 cut(s) | 34 |
| BtsCI | GGATG | 9 cut(s) | 752, 876, 1333, 1939, 2431, 2553, 3135, 3808, 4372 |
| BtsIMutI | CAGTG | 8 cut(s) | 355, 459, 713, 922, 1495, 2056, 2611, 3461 |
| BveI | ACCTGC | 3 cut(s) | 565, 3418, 3725 |
| Cac8I | GCNNGC | 7 cut(s) | 23, 76, 119, 554, 3765, 4014, 4146 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3308 |
| CciI | TCATGA | 1 cut(s) | 1605 |
| CfoI | GCGC | 6 cut(s) | 21, 58, 74, 219, 500, 3577 |
| Cfr10I | RCCGGY | 3 cut(s) | 74, 476, 610 |
| Cfr13I | GGNCC | 8 cut(s) | 211, 645, 790, 1435, 1674, 1824, 2684, 4073 |
| Cfr9I | CCCGGG | 1 cut(s) | 648 |
| CseI | GACGC | 2 cut(s) | 317, 492 |
| CsiI | ACCWGGT | 1 cut(s) | 2480 |
| CspAI | ACCGGT | 2 cut(s) | 476, 610 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 4201, 4236 |
| DinI | GGCGCC | 3 cut(s) | 57, 73, 499 |
| DraI | TTTAAA | 1 cut(s) | 2941 |
| DraIII | CACNNNGTG | 1 cut(s) | 1069 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 209 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 209 |
| EaeI | YGGCCR | 2 cut(s) | 76, 4059 |
| Eam1104I | CTCTTC | 8 cut(s) | 107, 1971, 3173, 3288, 4061, 4211, 4266, 4297 |
| EarI | CTCTTC | 8 cut(s) | 107, 1971, 3173, 3288, 4061, 4211, 4266, 4297 |
| EciI | GGCGGA | 1 cut(s) | 2105 |
| Ecl136II | GAGCTC | 3 cut(s) | 775, 2547, 3202 |
| Eco130I | CCWWGG | 9 cut(s) | 303, 751, 866, 2085, 2239, 2633, 2820, 3139, 3357 |
| Eco24I | GRGCYC | 3 cut(s) | 777, 2549, 3204 |
| Eco47I | GGWCC | 7 cut(s) | 211, 645, 790, 1435, 1824, 2684, 4073 |
| Eco47III | AGCGCT | 1 cut(s) | 218 |
| Eco53kI | GAGCTC | 3 cut(s) | 775, 2547, 3202 |
| Eco57I | CTGAAG | 5 cut(s) | 1292, 2951, 3294, 3894, 4095 |
| Eco81I | CCTNAGG | 2 cut(s) | 2892, 3705 |
| Eco88I | CYCGRG | 2 cut(s) | 648, 1775 |
| Eco91I | GGTNACC | 1 cut(s) | 319 |
| EcoICRI | GAGCTC | 3 cut(s) | 775, 2547, 3202 |
| EcoO109I | RGGNCCY | 1 cut(s) | 790 |
| EcoO65I | GGTNACC | 1 cut(s) | 319 |
| EcoRI | GAATTC | 2 cut(s) | 527, 3056 |
| EcoRII | CCWGG | 4 cut(s) | 390, 2008, 2134, 2480 |
| EcoT14I | CCWWGG | 9 cut(s) | 303, 751, 866, 2085, 2239, 2633, 2820, 3139, 3357 |
| EcoT22I | ATGCAT | 1 cut(s) | 2976 |
| EcoT38I | GRGCYC | 3 cut(s) | 777, 2549, 3204 |
| EgeI | GGCGCC | 3 cut(s) | 57, 73, 499 |
| EheI | GGCGCC | 3 cut(s) | 57, 73, 499 |
| ErhI | CCWWGG | 9 cut(s) | 303, 751, 866, 2085, 2239, 2633, 2820, 3139, 3357 |
| Esp3I | CGTCTC | 1 cut(s) | 229 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 1352, 1384, 1895, 1927, 2159, 2191, 3798, 3830 |
| FaqI | GGGAC | 7 cut(s) | 218, 776, 1428, 1448, 1889, 2350, 2548 |
| FauI | CCCGC | 2 cut(s) | 30, 1405 |
| FbaI | TGATCA | 4 cut(s) | 1602, 1650, 2023, 2857 |
| FokI | GGATG | 9 cut(s) | 759, 883, 1340, 1946, 2438, 2540, 3122, 3815, 4379 |
| FriOI | GRGCYC | 3 cut(s) | 777, 2549, 3204 |
| FspBI | CTAG | 7 cut(s) | 441, 1892, 2879, 2952, 3833, 3924, 4308 |
| GlaI | GCGC | 6 cut(s) | 20, 57, 73, 218, 499, 3576 |
| GsaI | CCCAGC | 1 cut(s) | 3267 |
| GsuI | CTGGAG | 7 cut(s) | 143, 1343, 2810, 3530, 3843, 4038, 4272 |
| HaeII | RGCGCY | 5 cut(s) | 22, 59, 75, 220, 501 |
| HaeIII | GGCC | 7 cut(s) | 78, 240, 1675, 2819, 3920, 4061, 4116 |
| HapII | CCGG | 8 cut(s) | 75, 237, 377, 413, 477, 611, 649, 4237 |
| HgaI | GACGC | 2 cut(s) | 317, 492 |
| HhaI | GCGC | 6 cut(s) | 21, 58, 74, 219, 500, 3577 |
| Hin1I | GRCGYC | 4 cut(s) | 56, 72, 330, 498 |
| Hin6I | GCGC | 6 cut(s) | 19, 56, 72, 217, 498, 3575 |
| HinP1I | GCGC | 6 cut(s) | 19, 56, 72, 217, 498, 3575 |
| HincII | GTYRAC | 3 cut(s) | 46, 63, 4243 |
| HindII | GTYRAC | 3 cut(s) | 46, 63, 4243 |
| HindIII | AAGCTT | 5 cut(s) | 1785, 1798, 2036, 2390, 3393 |
| HpaI | GTTAAC | 1 cut(s) | 4243 |
| HpaII | CCGG | 8 cut(s) | 75, 237, 377, 413, 477, 611, 649, 4237 |
| HphI | GGTGA | 9 cut(s) | 280, 313, 380, 664, 2255, 2621, 3035, 3160, 3724 |
| Hpy99I | CGWCG | 8 cut(s) | 206, 260, 332, 335, 371, 458, 551, 4415 |
| HpyCH4IV | ACGT | 4 cut(s) | 42, 231, 330, 1068 |
| HpySE526I | ACGT | 4 cut(s) | 42, 231, 330, 1068 |
| Hsp92I | GRCGYC | 4 cut(s) | 56, 72, 330, 498 |
| HspAI | GCGC | 6 cut(s) | 19, 56, 72, 217, 498, 3575 |
| KasI | GGCGCC | 3 cut(s) | 55, 71, 497 |
| KflI | GGGWCCC | 1 cut(s) | 790 |
| KpnI | GGTACC | 1 cut(s) | 2624 |
| KroI | GCCGGC | 1 cut(s) | 74 |
| KroNI | GCCGGC | 1 cut(s) | 76 |
| Ksp22I | TGATCA | 4 cut(s) | 1602, 1650, 2023, 2857 |
| KspAI | GTTAAC | 1 cut(s) | 4243 |
| LguI | GCTCTTC | 2 cut(s) | 107, 4297 |
| LweI | GCATC | 7 cut(s) | 354, 737, 1318, 1603, 2983, 3472, 4357 |
| MabI | ACCWGGT | 1 cut(s) | 2480 |
| MaeI | CTAG | 7 cut(s) | 441, 1892, 2879, 2952, 3833, 3924, 4308 |
| MaeII | ACGT | 4 cut(s) | 42, 231, 330, 1068 |
| MfeI | CAATTG | 2 cut(s) | 1944, 3363 |
| MflI | RGATCY | 8 cut(s) | 158, 249, 295, 1267, 2092, 2629, 2824, 3475 |
| MhlI | GDGCHC | 7 cut(s) | 129, 432, 777, 2374, 2549, 2963, 3204 |
| Mly113I | GGCGCC | 3 cut(s) | 56, 72, 498 |
| MlyI | GAGTC | 8 cut(s) | 919, 1489, 2332, 2761, 2801, 4100, 4352, 4394 |
| MmeI | TCCRAC | 4 cut(s) | 163, 3066, 3081, 3229 |
| Mph1103I | ATGCAT | 1 cut(s) | 2976 |
| MroNI | GCCGGC | 1 cut(s) | 74 |
| MroXI | GAANNNNTTC | 4 cut(s) | 1233, 1910, 3024, 3215 |
| MslI | CAYNNNNRTG | 6 cut(s) | 350, 1443, 2241, 2273, 2367, 2954 |
| MspA1I | CMGCKG | 7 cut(s) | 25, 121, 3731, 3977, 4040, 4079, 4298 |
| MspI | CCGG | 8 cut(s) | 75, 237, 377, 413, 477, 611, 649, 4237 |
| MspR9I | CCNGG | 8 cut(s) | 237, 392, 649, 650, 2010, 2136, 2482, 4238 |
| MunI | CAATTG | 2 cut(s) | 1944, 3363 |
| Mva1269I | GAATGC | 2 cut(s) | 2224, 3577 |
| MvaI | CCWGG | 4 cut(s) | 392, 2010, 2136, 2482 |
| MvnI | CGCG | 1 cut(s) | 53 |
| NaeI | GCCGGC | 1 cut(s) | 76 |
| NarI | GGCGCC | 3 cut(s) | 56, 72, 498 |
| NciI | CCSGG | 4 cut(s) | 237, 649, 650, 4238 |
| NcoI | CCATGG | 4 cut(s) | 303, 2085, 2239, 3357 |
| NgoMIV | GCCGGC | 1 cut(s) | 74 |
| NmuCI | GTSAC | 7 cut(s) | 319, 652, 917, 991, 2051, 3459, 4355 |
| NsiI | ATGCAT | 1 cut(s) | 2976 |
| NspI | RCATGY | 1 cut(s) | 2974 |
| OliI | CACNNNNGTG | 1 cut(s) | 2241 |
| PagI | TCATGA | 1 cut(s) | 1605 |
| PaqCI | CACCTGC | 1 cut(s) | 565 |
| PciSI | GCTCTTC | 2 cut(s) | 107, 4297 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 201, 3939 |
| PctI | GAATGC | 2 cut(s) | 2224, 3577 |
| PdiI | GCCGGC | 1 cut(s) | 76 |
| PdmI | GAANNNNTTC | 4 cut(s) | 1233, 1910, 3024, 3215 |
| PinAI | ACCGGT | 2 cut(s) | 476, 610 |
| Ple19I | CGATCG | 1 cut(s) | 93 |
| PleI | GAGTC | 8 cut(s) | 918, 1489, 2332, 2761, 2800, 4099, 4352, 4393 |
| PluTI | GGCGCC | 3 cut(s) | 59, 75, 501 |
| PpsI | GAGTC | 8 cut(s) | 918, 1489, 2332, 2761, 2800, 4099, 4352, 4393 |
| PpuMI | RGGWCCY | 1 cut(s) | 790 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1499 |
| PshBI | ATTAAT | 1 cut(s) | 2307 |
| PsiI | TTATAA | 3 cut(s) | 1715, 2565, 2778 |
| Psp124BI | GAGCTC | 3 cut(s) | 777, 2549, 3204 |
| Psp5II | RGGWCCY | 1 cut(s) | 790 |
| Psp6I | CCWGG | 4 cut(s) | 390, 2008, 2134, 2480 |
| PspEI | GGTNACC | 1 cut(s) | 319 |
| PspFI | CCCAGC | 1 cut(s) | 3263 |
| PspGI | CCWGG | 4 cut(s) | 390, 2008, 2134, 2480 |
| PspPI | GGNCC | 8 cut(s) | 211, 645, 790, 1435, 1674, 1824, 2684, 4073 |
| PspPPI | RGGWCCY | 1 cut(s) | 790 |
| PstI | CTGCAG | 3 cut(s) | 30, 1551, 3988 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3308 |
| PsuI | RGATCY | 8 cut(s) | 158, 249, 295, 1267, 2092, 2629, 2824, 3475 |
| PvuI | CGATCG | 1 cut(s) | 93 |
| PvuII | CAGCTG | 3 cut(s) | 121, 3977, 4298 |
| RseI | CAYNNNNRTG | 6 cut(s) | 350, 1443, 2241, 2273, 2367, 2954 |
| SacI | GAGCTC | 3 cut(s) | 777, 2549, 3204 |
| SapI | GCTCTTC | 2 cut(s) | 107, 4297 |
| Sau96I | GGNCC | 8 cut(s) | 211, 645, 790, 1435, 1674, 1824, 2684, 4073 |
| ScaI | AGTACT | 2 cut(s) | 1858, 2866 |
| SchI | GAGTC | 8 cut(s) | 919, 1489, 2332, 2761, 2801, 4100, 4352, 4394 |
| ScrFI | CCNGG | 8 cut(s) | 237, 392, 649, 650, 2010, 2136, 2482, 4238 |
| SduI | GDGCHC | 7 cut(s) | 129, 432, 777, 2374, 2549, 2963, 3204 |
| SexAI | ACCWGGT | 1 cut(s) | 2480 |
| SfaNI | GCATC | 7 cut(s) | 354, 737, 1318, 1603, 2983, 3472, 4357 |
| SfcI | CTRYAG | 4 cut(s) | 26, 1547, 2868, 3984 |
| SfoI | GGCGCC | 3 cut(s) | 57, 73, 499 |
| SgrAI | CRCCGGYG | 1 cut(s) | 476 |
| SinI | GGWCC | 7 cut(s) | 211, 645, 790, 1435, 1824, 2684, 4073 |
| SmaI | CCCGGG | 1 cut(s) | 650 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 350, 1443, 2241, 2273, 2367, 2954 |
| SmlI | CTYRAG | 9 cut(s) | 314, 1055, 1497, 1801, 2769, 2802, 3029, 3095, 3197 |
| SmoI | CTYRAG | 9 cut(s) | 314, 1055, 1497, 1801, 2769, 2802, 3029, 3095, 3197 |
| SpeI | ACTAGT | 1 cut(s) | 440 |
| SspDI | GGCGCC | 3 cut(s) | 55, 71, 497 |
| SspI | AATATT | 2 cut(s) | 1528, 2590 |
| SspMI | CTAG | 7 cut(s) | 441, 1892, 2879, 2952, 3833, 3924, 4308 |
| SstI | GAGCTC | 3 cut(s) | 777, 2549, 3204 |
| StyD4I | CCNGG | 8 cut(s) | 235, 390, 647, 648, 2008, 2134, 2480, 4236 |
| StyI | CCWWGG | 9 cut(s) | 303, 751, 866, 2085, 2239, 2633, 2820, 3139, 3357 |
| TaiI | ACGT | 4 cut(s) | 45, 234, 333, 1071 |
| TaqI | TCGA | 8 cut(s) | 9, 93, 195, 255, 327, 333, 771, 3829 |
| TaqII | GACCGA | 1 cut(s) | 1625 |
| TatI | WGTACW | 5 cut(s) | 354, 567, 1856, 2046, 2864 |
| TauI | GCSGC | 8 cut(s) | 56, 278, 559, 3734, 3883, 4043, 4061, 4082 |
| TscAI | CASTG | 8 cut(s) | 355, 466, 720, 922, 1495, 2056, 2611, 3468 |
| TseFI | GTSAC | 7 cut(s) | 319, 652, 917, 991, 2051, 3459, 4355 |
| Tsp45I | GTSAC | 7 cut(s) | 319, 652, 917, 991, 2051, 3459, 4355 |
| TspGWI | ACGGA | 6 cut(s) | 211, 288, 384, 1394, 2747, 3733 |
| TspMI | CCCGGG | 1 cut(s) | 648 |
| TspRI | CASTG | 8 cut(s) | 355, 466, 720, 922, 1495, 2056, 2611, 3468 |
| VpaK11BI | GGWCC | 7 cut(s) | 211, 645, 790, 1435, 1824, 2684, 4073 |
| VspI | ATTAAT | 1 cut(s) | 2307 |
| XapI | RAATTY | 6 cut(s) | 527, 677, 2783, 2874, 3024, 3056 |
| XceI | RCATGY | 1 cut(s) | 2974 |
| XcmI | CCANNNNNNNNNTGG | 5 cut(s) | 2272, 2631, 2760, 3462, 3682 |
| XmaI | CCCGGG | 1 cut(s) | 648 |
| XmnI | GAANNNNTTC | 4 cut(s) | 1233, 1910, 3024, 3215 |
| XspI | CTAG | 7 cut(s) | 441, 1892, 2879, 2952, 3833, 3924, 4308 |
| ZraI | GACGTC | 1 cut(s) | 331 |
| ZrmI | AGTACT | 2 cut(s) | 1858, 2866 |
| Zsp2I | ATGCAT | 1 cut(s) | 2976 |