FvH4_7g01280

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001532
Physical Location & Seq
Reverse (-)
1694082 .. 1701404
7323 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01280.t2

Sequence Viewer

Length: 4410 bp
ATGGTGGTGGAGAAGAAGCGTCCGCTGCAGAGCAGCAACAACGCCAACACGGCGGCAGCGATGGACGGCAAGACGATCGAGGAAATGTACCAGAAGAAGAGCCAGCTGGAGCACATCTTGCTCCGGCCCGATACCTATATCGGATCCATCGAGAAGCACACCCAGACGCTTTGGGTCTTCGAGAGCGACGACATGGTCCACCGCTCCGTCTCCTACGTCCCCGGCCTTTACAAGATCTTCGACGAGATTCTCGTCAACGCCGCCGACAACAAGCAGCGAGATCCGTCCATGGACGCTCTCAAGGTCACAATCGACGTCGACCAGAACTGCATCAGTGTCTATAACAACGGCGACGGAGTTCCGGTCGAGATTCACCAGGAGGAGAAGGTCTATGTGCCGGAGTTGATCTTCGGTCATCTCCTGACTAGTAGTAACTACGACGACACCGTCAAGAAGACCACCGGTGGACGCAACGGCTACGGCGCCAAGCTCACTAATATATTCTCCACCGAATTCGTCATCGAAACCGCCGACGGCAAGCGGCAGAAGAAGTACAAGCAGGTGTTCACAAACAACATGGGGAAGAAATCTGAACCAGTCATCACTAAATGCAAAGAGAGTGAGAACTGGACCCGAGTGACATATAAGCCTGACTTGACTAAATTTAACATGACATATCTGGAAGAAGATGTGGTTGCATTGATGAAAAAGCGAGTGATTGACATGGCTGGATGCCTCGGAAAGACTGTGAAGGTTGAGTTGAATGGAAAGAGGGTCCCTGTAAAGTCGTTTCTTGATTATGTTGATCTCTACTTGCAATCTGCTGGGAAATCTAAAGATATTAAACTTCCAAGGATGACTGAGAAAGTCAATGATAGGTGGGAGATTTGTGTGAGTCTCAGCGATGGCCAGTTTCAACAGGTCAGCTTTGTGAATAGCATAGCTACAATCAAGGGGGGAACTCATGTTGATTATGTCACGAATCAGATAACCAATCATGTGATGAATGTTGTAAATAAGAAGAATAAGAATGCCAACCTCAAGGCTCACAATGTGAAGAATTATCTTTGGGTCTTTGTCAATGCTCTTATTGACAACCCTGCCTTCGATTCCCAAACAAAGGAAACTTTGACGATTCGCCCAAGCAGTTTTGGGTCTAAATGTGAACTACCACAAGAGTTTTTAAAGAAAGTTGCGAAGTCTGGGGTAGTGGAAAGTCTTCTCTCTTGGGCAAATTTCAAAAATAACAAAGATCTGAAGAAAACTGATGGAACAAAAACAGAGAGACTTCATAACATCAACAAGTTGGAGGATGCCAACTTAGCAGGAGGAAGGAACTCTGAAAAGTGTACCTTGATTTTAACAGAAGGAGATTCAGCTAAGGCTCTTGCTATGGCGGGGCTTGCTGTTGTTGGCCGGGACCATTATGGTGTTTTTCCATTGAGAGGTAAATTGCTCAATGTAAGAGAAGCCAGCACGACTCAACTCAGGGATAATGAAGAAATCAAGAATATTAAGAAGATTCTTGGTCTGCAACAAGATAAAGAATATACCAACATCAAATCTTTAAGATATGGGCATCTGATGATCATGACTGATCAGGATCACGATGGTTCCCATATAAAAGGGTTGCTGATCAATTTCATTCATTCCTTCTGGCCCTCTCTGCTAAAAGTTCCATCATTTTTGGTTGAGTTTATAACGCCTATTGTTAAGGCAACTCATAAGAATGGGAAAGAACTTGCATTTTATTCAATGCCTGAGTATGAAGCTTGGAGGGAAAGCTTGAGGGGTAATGCAAGTGGCTGGACCATTAAATACTATAAGGGGTTGGGAACAAGTACTTCAAAGGAAGGTAGGGACTACTTTCAGAATCTAGATATGCACAAGAAAGACTTTGTTTGGATAGATGAGCGGGATGGGGAGGCAATTGAACTTGCTTTCAGTAAGAAGAAAATAGAAGAGAGGAAGAATTGGCTTCGGCAGTTTGAGCCTGGTACTCATCTTGATCAGAAGGAGAAGCTTGTAAAGTACAGTGATTTTGTTCATAAGGAGCTCATACAGTTTTCCATGGCGGATCTGCAGAGGTCAATACCATCAATGGTTGATGGGTTGAAGCCAGGTCAAAGAAAGATACTATTCTGCTCCTTTAAGAGGAACTTTATAAAGGAAGCAAAAGTTGCACAGTTTTCTGGTTATGTGTCTGAGCATTCTGCCTACCACCATGGTGAGCAAAGTCTTGCGAGTACTATCATTGGAATGGCACAAGATTATGTGGGCAGCAACAACATCAATTTCCTTCAACCAAATGGTCAATTTGGGACCCGTAACTTGGGAGGCAAAGACCATGCAAGTGCTCGTTATATATATACTCGGCTTTCTCCTATTACAAGGTTTCTTTTTCCAAAGGATGATGATAGACTTCTTGACTACTTGAATGAAGATGGGCAATCAATAGAGCCAACCTGGTATGTGCCAATTATCCCAACGGTTCTGGTGAATGGTAGTGAAGGAATTGGGACAGGGTGGAGCTCTTACATCCCTAATTATAATCCAAGAGACATCGTTGCAAATGTAAGGCGGTTGCTCAATGGTGATGAAATGGTTCCAATGGATCCATGGTATAGAGGCTTTACTGGATTTATTGAGAAAACTGTCAAGGAAACTGGTACGAGCTACACAGCATGCGGGATAATTGAGGAAGTCAACGAGACAACACTCAGAATTTCGGAGCTGCCAATCCGTAGGTGGACTCAAGATTATAAGGAATTTCTGGAGTCTATCTCTCAAGGGAATGACAAGGCGAAGGATCCTTTCATTGAGGGTTTCACACAGCATAGTGATCACAGTACTGTTGAAATTCTAGTCCACTTGCCTGAGGAGAACTTGATGGCAGCCAGAAAAGAGGGTTTGCTGAAGAAATTCAGACTGACCACTAGCATAGCCACAACTAACATGCACCTGTTTGATCCAAAGGGTGTTATCAAGAAATATGACACTGCTGAACAAATTCTTGAGGAGTTCTTTCCCTTAAGACTTGAATTCTATGAGAAACGAAAGAAAGTGTTGTTGGATAACCTTGAGTTGGAGTTGTTGAAAATGGAAAACAAGGTTAGATTCATCCTTGGTGTGGTGAATGGAGAGATTATTGTGAGCAATAGGAAGAGAGCTGATCTATTCCTTGAGCTCCAACAGAAAGGTTTCACTCCTTTCCCAAAGAAAACAAAGTCTGTGGAGCCAGAGGTTGCTGGGGCAACTGAAGATACAGAAGAAACAGAAGAGAACTCAGAGACTGTCATTAGTAATGGAGTACGAATCAGCGATTATGAGTATCTAATTTCCATGGCAATTGGAACCTTGACCATTGAGAGGGTTGAAGCTTTATTGGCTGATAGAGATAAGGTCAATGAGCAGGTTAATGATTTGAGAGGTTCAACTCCAAAGTCATTGTGGTTGAAGGATCTGGATGTTCTTGAAGCGGAACTTAATGAGCTGGAGAAAAGTGAGGCTCTGGCAGATGAGGCAAAAAGGAAATCAAGAAACAACCAAATCAAGAATGCTCCGGTTGGGAAGGTTTCTAAACAAGCACCTAAACCTCGAAAGAACACCAAAAAGGTTAACAATGCAGAAGCTATTCCAGAAAGTAGGGAAACGTCTTCCAGTTCTGCAATGGAGATTGAGAAACCTCCTGAGGTGATGAAACGGAGAGGTGTGGCAGCAGGTTCAAAGAAAGCTCCTGCTAAGAAGCAAGTAAAATCCACAGTAGTTTTGTCTGATGACGATGATGATGAAGAAATGCTTGATTTGAAAGATCGGCTTGCTGCCTATAATCTTGATTCATCTCCAGATAAGGAAACTGAAGTGCCACTAAAACCAGCCCAGAAGAAAGAAGCCAGCAAAAAGGCCACACTAAAAGACGACGAGGATGATGATGACGATGATTTTGAGGTGGAGATAGCAGCTGCCCCCGCAGCAGTAAAGAAGGGAGGCAGAAAGCCTGCTGGAGCTGCAAAGGCAGCAGCAGCGGCTAAGCCCCCTGTGGCAGCCAAGAAGAGAGGCGCAGCGGCTAAGAAGGACACTCAAACATCGTCTTCTTCAGGCCAGAAGCTCTTAACCGATATGTTGAAGCCTGCTGCTGAGAGTTCCGGGGTTTCACCTGACCCCAAAGTGAGAAAGATGAGGGAATCTCCATTCAACAAGAAGAGTGGCTCTGTGTTGAGCAGGGTTAACTTGGAAGATGAGATGAATGTAAGTGAAGAGAAAACCGAAGCTCCGGCAGCTGGAAGAGCTAGGCCACAGAGGGCAGCTGCTCGTAATAGGAAAATGATTCTGGTGAGTGACTCTGAGGAGGATGCAAATGATAGTGATGAGTCTGAGGTGGAGGAAATTGAGTCCGACGATTCTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 map01524
Pfam Domains
Protein Families
DNA_gyraseB DNA_topoisoIV HATPase_c TOPRIM_C Toprim

Protein Analysis

1470

Amino Acids

165.36

Weight (kDa)

6.76

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_gyraseB PF00204 5 - 136 4.9e-23 DNA gyrase B
Toprim PF01751 166 - 265 8e-09 Toprim domain
TOPRIM_C PF16898 281 - 408 1.4e-51 C-terminal associated domain of TOPRIM
DNA_topoisoIV PF00521 411 - 870 7.3e-118 DNA gyrase/topoisomerase IV, subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1700, 2165, 2550, 2763
AarI CACCTGC 1 cut(s) 550
AasI GACNNNNNNGTC 2 cut(s) 194, 446
AatII GACGTC 1 cut(s) 318
Acc36I ACCTGC 3 cut(s) 550, 3403, 3710
AccB1I GGYRCC 1 cut(s) 482
AccBSI CCGCTC 2 cut(s) 204, 1915
AccI GTMKAC 1 cut(s) 318
AcoI YGGCCR 2 cut(s) 907, 1414
AcsI RAATTY 9 cut(s) 512, 662, 1234, 2724, 2768, 2859, 2921, 3009, 3041
AcuI CTGAAG 5 cut(s) 1277, 2936, 3279, 3879, 4080
AcyI GRCGYC 2 cut(s) 315, 483
AdeI CACNNNGTG 1 cut(s) 1054
AfiI CCNNNNNNNGG 8 cut(s) 1120, 1445, 2154, 2332, 3085, 3130, 3369, 4280
AflII CTTAAG 1 cut(s) 3031
AgeI ACCGGT 1 cut(s) 461
AhlI ACTAGT 1 cut(s) 425
AjnI CCWGG 4 cut(s) 375, 1993, 2119, 2465
AjuI GAANNNNNNNTTGG 2 cut(s) 3053, 3085
AleI CACNNNNGTG 1 cut(s) 2226
Alw21I GWGCWC 5 cut(s) 114, 2058, 2359, 2534, 3189
Alw26I GTCTC 6 cut(s) 214, 902, 1279, 2553, 2705, 3284
AlwNI CAGNNNCTG 1 cut(s) 3293
Ama87I CYCGRG 1 cut(s) 633
AoxI GGCC 8 cut(s) 125, 223, 907, 1414, 1658, 3903, 4099, 4292
ApoI RAATTY 9 cut(s) 512, 662, 1234, 2724, 2768, 2859, 2921, 3009, 3041
ArsI GACNNNNNNTTYG 2 cut(s) 3926, 3958
AsiGI ACCGGT 1 cut(s) 461
Asp700I GAANNNNTTC 6 cut(s) 1218, 2604, 2921, 3009, 3200, 3633
AspLEI GCGC 2 cut(s) 485, 4061
AspS9I GGNCC 8 cut(s) 126, 196, 630, 775, 1420, 1659, 1809, 2322
AsuC2I CCSGG 3 cut(s) 222, 1418, 4147
AsuHPI GGTGA 8 cut(s) 365, 2240, 2509, 2606, 3145, 3706, 4146, 4345
AvaI CYCGRG 1 cut(s) 633
AvaII GGWCC 6 cut(s) 196, 630, 775, 1420, 1809, 2322
AxyI CCTNAGG 2 cut(s) 2877, 3690
BalI TGGCCA 1 cut(s) 909
BamHI GGATCC 3 cut(s) 143, 2614, 2809
BanI GGYRCC 1 cut(s) 482
BanII GRGCYC 3 cut(s) 2058, 2534, 3189
BbsI GAAGAC 5 cut(s) 169, 461, 1211, 3648, 4083
Bbv12I GWGCWC 5 cut(s) 114, 2058, 2359, 2534, 3189
BceAI ACGGC 6 cut(s) 66, 82, 364, 490, 496, 550
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciT130I CCWGG 4 cut(s) 377, 1995, 2121, 2467
BclI TGATCA 5 cut(s) 1587, 1597, 1635, 2008, 2842
BcnI CCSGG 3 cut(s) 222, 1418, 4147
BcoDI GTCTC 6 cut(s) 214, 902, 1279, 2553, 2705, 3284
BcuI ACTAGT 1 cut(s) 425
BfaI CTAG 5 cut(s) 426, 1877, 2864, 2937, 4290
BfmI CTRYAG 2 cut(s) 26, 2081
BfoI RGCGCY 1 cut(s) 486
BfrI CTTAAG 1 cut(s) 3031
BfuAI ACCTGC 3 cut(s) 550, 3403, 3710
BglI GCCNNNNNGGC 1 cut(s) 50
BglII AGATCT 2 cut(s) 234, 1252
BlpI GCTNAGC 1 cut(s) 4029
BmcAI AGTACT 3 cut(s) 1843, 2248, 2851
Bme1390I CCNGG 7 cut(s) 222, 377, 1418, 1995, 2121, 2467, 4147
Bme18I GGWCC 6 cut(s) 196, 630, 775, 1420, 1809, 2322
BmeT110I CYCGRG 1 cut(s) 633
BmgT120I GGNCC 8 cut(s) 126, 196, 630, 775, 1420, 1659, 1809, 2322
BmrFI CCNGG 7 cut(s) 222, 377, 1418, 1995, 2121, 2467, 4147
BmsI GCATC 5 cut(s) 339, 722, 1303, 1588, 4342
BpiI GAAGAC 5 cut(s) 169, 461, 1211, 3648, 4083
BplI GAGNNNNNCTC 4 cut(s) 2768, 2800, 4171, 4203
BpmI CTGGAG 5 cut(s) 128, 2795, 3515, 3828, 4023
Bpu10I CCTNAGC 1 cut(s) 1380
Bpu1102I GCTNAGC 1 cut(s) 4029
BpuEI CTTGAG 8 cut(s) 284, 1025, 1807, 2739, 2772, 3035, 3101, 3203
BpuMI CCSGG 3 cut(s) 222, 1418, 4147
BsaBI GATNNNNATC 1 cut(s) 1602
BsaHI GRCGYC 2 cut(s) 315, 483
BsaWI WCCGGW 3 cut(s) 361, 461, 3562
BsaXI ACNNNNNCTCC 4 cut(s) 348, 378, 4255, 4285
Bsc4I CCNNNNNNNGG 8 cut(s) 1120, 1445, 2154, 2332, 3085, 3130, 3369, 4280
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 6 cut(s) 596, 632, 910, 2641, 2671, 3660
Bse21I CCTNAGG 2 cut(s) 2877, 3690
Bse3DI GCAATG 1 cut(s) 3675
Bse8I GATNNNNATC 1 cut(s) 1602
BseBI CCWGG 4 cut(s) 377, 1995, 2121, 2467
BseJI GATNNNNATC 1 cut(s) 1602
BseLI CCNNNNNNNGG 8 cut(s) 1120, 1445, 2154, 2332, 3085, 3130, 3369, 4280
BseMI GCAATG 1 cut(s) 3675
BseNI ACTGG 6 cut(s) 596, 632, 910, 2641, 2671, 3660
BseRI GAGGAG 4 cut(s) 395, 2894, 3032, 4361
BseYI CCCAGC 2 cut(s) 824, 3248
Bsh1285I CGRYCG 2 cut(s) 78, 366
BshFI GGCC 8 cut(s) 127, 225, 909, 1416, 1660, 3905, 4101, 4294
BshNI GGYRCC 1 cut(s) 482
BshTI ACCGGT 1 cut(s) 461
BsiEI CGRYCG 2 cut(s) 78, 366
BsiHKAI GWGCWC 5 cut(s) 114, 2058, 2359, 2534, 3189
BsiHKCI CYCGRG 1 cut(s) 633
BsiSI CCGG 9 cut(s) 124, 222, 362, 398, 462, 1417, 3563, 4146, 4274
BslFI GGGAC 6 cut(s) 203, 761, 1433, 1874, 2335, 2533
BslI CCNNNNNNNGG 8 cut(s) 1120, 1445, 2154, 2332, 3085, 3130, 3369, 4280
BsmAI GTCTC 6 cut(s) 214, 902, 1279, 2553, 2705, 3284
BsmBI CGTCTC 1 cut(s) 214
BsmFI GGGAC 6 cut(s) 203, 761, 1433, 1874, 2335, 2533
BsmI GAATGC 3 cut(s) 1036, 2209, 3562
BsnI GGCC 8 cut(s) 127, 225, 909, 1416, 1660, 3905, 4101, 4294
BsoBI CYCGRG 1 cut(s) 633
Bsp1286I GDGCHC 5 cut(s) 114, 2058, 2359, 2534, 3189
Bsp1720I GCTNAGC 1 cut(s) 4029
Bsp19I CCATGG 5 cut(s) 288, 2070, 2224, 2618, 3342
BspANI GGCC 8 cut(s) 127, 225, 909, 1416, 1660, 3905, 4101, 4294
BspHI TCATGA 1 cut(s) 1590
BspMAI CTGCAG 2 cut(s) 30, 2085
BspMI ACCTGC 3 cut(s) 550, 3403, 3710
BspQI GCTCTTC 2 cut(s) 92, 4279
BspT107I GGYRCC 1 cut(s) 482
BspTI CTTAAG 1 cut(s) 3031
BsrBI CCGCTC 2 cut(s) 204, 1915
BsrDI GCAATG 1 cut(s) 3675
BsrFI RCCGGY 1 cut(s) 461
BsrI ACTGG 6 cut(s) 596, 632, 910, 2641, 2671, 3660
BssAI RCCGGY 1 cut(s) 461
BssNI GRCGYC 2 cut(s) 315, 483
BssT1I CCWWGG 7 cut(s) 288, 851, 2070, 2224, 2618, 3124, 3342
Bst2UI CCWGG 4 cut(s) 377, 1995, 2121, 2467
Bst6I CTCTTC 8 cut(s) 92, 1956, 3158, 3273, 4046, 4196, 4251, 4279
BstACI GRCGYC 2 cut(s) 315, 483
BstAFI CTTAAG 1 cut(s) 3031
BstAPI GCANNNNNTGC 2 cut(s) 118, 2180
BstC8I GCNNGC 9 cut(s) 104, 539, 1404, 1474, 2686, 3819, 3895, 3999, 4131
BstDSI CCRYGG 5 cut(s) 288, 2070, 2224, 2618, 3342
BstENI CCTNNNNNAGG 1 cut(s) 2152
BstH2I RGCGCY 1 cut(s) 486
BstHHI GCGC 2 cut(s) 485, 4061
BstMAI GTCTC 6 cut(s) 214, 902, 1279, 2553, 2705, 3284
BstMCI CGRYCG 2 cut(s) 78, 366
BstNI CCWGG 4 cut(s) 377, 1995, 2121, 2467
BstNSI RCATGY 2 cut(s) 2688, 2959
BstSCI CCNGG 7 cut(s) 220, 375, 1416, 1993, 2119, 2465, 4145
BstSFI CTRYAG 2 cut(s) 26, 2081
BstV2I GAAGAC 5 cut(s) 169, 461, 1211, 3648, 4083
BstX2I RGATCY 8 cut(s) 143, 234, 280, 1252, 2077, 2614, 2809, 3460
BstYI RGATCY 8 cut(s) 143, 234, 280, 1252, 2077, 2614, 2809, 3460
Bsu36I CCTNAGG 2 cut(s) 2877, 3690
BsuRI GGCC 8 cut(s) 127, 225, 909, 1416, 1660, 3905, 4101, 4294
BtgI CCRYGG 5 cut(s) 288, 2070, 2224, 2618, 3342
BtgZI GCGATG 2 cut(s) 74, 918
BtsI GCAGTG 1 cut(s) 2997
BtsIMutI CAGTG 3 cut(s) 340, 2041, 2997
BveI ACCTGC 3 cut(s) 550, 3403, 3710
Cac8I GCNNGC 9 cut(s) 104, 539, 1404, 1474, 2686, 3819, 3895, 3999, 4131
CaiI CAGNNNCTG 1 cut(s) 3293
CciI TCATGA 1 cut(s) 1590
CfoI GCGC 2 cut(s) 485, 4061
Cfr10I RCCGGY 1 cut(s) 461
Cfr13I GGNCC 8 cut(s) 126, 196, 630, 775, 1420, 1659, 1809, 2322
CseI GACGC 4 cut(s) 8, 175, 302, 477
CsiI ACCWGGT 1 cut(s) 2465
CspAI ACCGGT 1 cut(s) 461
CspCI CAANNNNNGTGG 4 cut(s) 3213, 3248, 4186, 4221
DinI GGCGCC 1 cut(s) 484
DraI TTTAAA 1 cut(s) 1185
DraIII CACNNNGTG 1 cut(s) 1054
DrdI GACNNNNNNGTC 2 cut(s) 194, 446
DseDI GACNNNNNNGTC 2 cut(s) 194, 446
EaeI YGGCCR 2 cut(s) 907, 1414
Eam1104I CTCTTC 8 cut(s) 92, 1956, 3158, 3273, 4046, 4196, 4251, 4279
EarI CTCTTC 8 cut(s) 92, 1956, 3158, 3273, 4046, 4196, 4251, 4279
EciI GGCGGA 1 cut(s) 2090
Ecl136II GAGCTC 3 cut(s) 2056, 2532, 3187
Eco130I CCWWGG 7 cut(s) 288, 851, 2070, 2224, 2618, 3124, 3342
Eco24I GRGCYC 3 cut(s) 2058, 2534, 3189
Eco47I GGWCC 6 cut(s) 196, 630, 775, 1420, 1809, 2322
Eco53kI GAGCTC 3 cut(s) 2056, 2532, 3187
Eco57I CTGAAG 5 cut(s) 1277, 2936, 3279, 3879, 4080
Eco81I CCTNAGG 2 cut(s) 2877, 3690
Eco88I CYCGRG 1 cut(s) 633
EcoICRI GAGCTC 3 cut(s) 2056, 2532, 3187
EcoNI CCTNNNNNAGG 1 cut(s) 2152
EcoO109I RGGNCCY 2 cut(s) 775, 2322
EcoRI GAATTC 2 cut(s) 512, 3041
EcoRII CCWGG 4 cut(s) 375, 1993, 2119, 2465
EcoT14I CCWWGG 7 cut(s) 288, 851, 2070, 2224, 2618, 3124, 3342
EcoT38I GRGCYC 3 cut(s) 2058, 2534, 3189
EgeI GGCGCC 1 cut(s) 484
EheI GGCGCC 1 cut(s) 484
ErhI CCWWGG 7 cut(s) 288, 851, 2070, 2224, 2618, 3124, 3342
Esp3I CGTCTC 1 cut(s) 214
FaqI GGGAC 6 cut(s) 203, 761, 1433, 1874, 2335, 2533
FauI CCCGC 4 cut(s) 1390, 1908, 2681, 3976
FbaI TGATCA 5 cut(s) 1587, 1597, 1635, 2008, 2842
FblI GTMKAC 1 cut(s) 318
FriOI GRGCYC 3 cut(s) 2058, 2534, 3189
FspBI CTAG 5 cut(s) 426, 1877, 2864, 2937, 4290
GlaI GCGC 2 cut(s) 484, 4060
GsaI CCCAGC 2 cut(s) 828, 3252
GsuI CTGGAG 5 cut(s) 128, 2795, 3515, 3828, 4023
HaeII RGCGCY 1 cut(s) 486
HaeIII GGCC 8 cut(s) 127, 225, 909, 1416, 1660, 3905, 4101, 4294
HapII CCGG 9 cut(s) 124, 222, 362, 398, 462, 1417, 3563, 4146, 4274
HgaI GACGC 4 cut(s) 8, 175, 302, 477
HhaI GCGC 2 cut(s) 485, 4061
Hin1I GRCGYC 2 cut(s) 315, 483
Hin6I GCGC 2 cut(s) 483, 4059
HinP1I GCGC 2 cut(s) 483, 4059
HincII GTYRAC 5 cut(s) 256, 319, 2707, 3619, 4228
HindII GTYRAC 5 cut(s) 256, 319, 2707, 3619, 4228
HindIII AAGCTT 4 cut(s) 1770, 1783, 2021, 3378
HpaI GTTAAC 2 cut(s) 3619, 4228
HpaII CCGG 9 cut(s) 124, 222, 362, 398, 462, 1417, 3563, 4146, 4274
HphI GGTGA 8 cut(s) 365, 2240, 2509, 2606, 3145, 3706, 4146, 4345
Hpy99I CGWCG 9 cut(s) 191, 245, 317, 320, 356, 443, 536, 3923, 4400
HpyCH4IV ACGT 3 cut(s) 216, 315, 3653
HpySE526I ACGT 3 cut(s) 216, 315, 3653
Hsp92I GRCGYC 2 cut(s) 315, 483
HspAI GCGC 2 cut(s) 483, 4059
KasI GGCGCC 1 cut(s) 482
KflI GGGWCCC 2 cut(s) 775, 2322
Ksp22I TGATCA 5 cut(s) 1587, 1597, 1635, 2008, 2842
KspAI GTTAAC 2 cut(s) 3619, 4228
LguI GCTCTTC 2 cut(s) 92, 4279
LweI GCATC 5 cut(s) 339, 722, 1303, 1588, 4342
MabI ACCWGGT 1 cut(s) 2465
MaeI CTAG 5 cut(s) 426, 1877, 2864, 2937, 4290
MaeII ACGT 3 cut(s) 216, 315, 3653
MaeIII GTNAC 6 cut(s) 304, 431, 637, 976, 2327, 4337
MbiI CCGCTC 2 cut(s) 204, 1915
MfeI CAATTG 2 cut(s) 1929, 3348
MflI RGATCY 8 cut(s) 143, 234, 280, 1252, 2077, 2614, 2809, 3460
MhlI GDGCHC 5 cut(s) 114, 2058, 2359, 2534, 3189
MlsI TGGCCA 1 cut(s) 909
MluNI TGGCCA 1 cut(s) 909
Mly113I GGCGCC 1 cut(s) 483
MlyI GAGTC 7 cut(s) 904, 1474, 2746, 2786, 4334, 4379, 4400
MmeI TCCRAC 4 cut(s) 1287, 3051, 3066, 3214
Mox20I TGGCCA 1 cut(s) 909
MroXI GAANNNNTTC 6 cut(s) 1218, 2604, 2921, 3009, 3200, 3633
MscI TGGCCA 1 cut(s) 909
MslI CAYNNNNRTG 5 cut(s) 1428, 1728, 2226, 2258, 2352
Msp20I TGGCCA 1 cut(s) 909
MspA1I CMGCKG 7 cut(s) 25, 106, 3962, 4025, 4064, 4280, 4307
MspCI CTTAAG 1 cut(s) 3031
MspI CCGG 9 cut(s) 124, 222, 362, 398, 462, 1417, 3563, 4146, 4274
MspR9I CCNGG 7 cut(s) 222, 377, 1418, 1995, 2121, 2467, 4147
MunI CAATTG 2 cut(s) 1929, 3348
Mva1269I GAATGC 3 cut(s) 1036, 2209, 3562
MvaI CCWGG 4 cut(s) 377, 1995, 2121, 2467
NarI GGCGCC 1 cut(s) 483
NciI CCSGG 3 cut(s) 222, 1418, 4147
NcoI CCATGG 5 cut(s) 288, 2070, 2224, 2618, 3342
NmeAIII GCCGAG 1 cut(s) 2353
NmuCI GTSAC 4 cut(s) 304, 637, 976, 4337
NspI RCATGY 2 cut(s) 2688, 2959
OliI CACNNNNGTG 1 cut(s) 2226
PaeI GCATGC 1 cut(s) 2688
PagI TCATGA 1 cut(s) 1590
PaqCI CACCTGC 1 cut(s) 550
PciSI GCTCTTC 2 cut(s) 92, 4279
PcsI WCGNNNNNNNCGW 6 cut(s) 56, 147, 186, 249, 444, 528
PctI GAATGC 3 cut(s) 1036, 2209, 3562
PdmI GAANNNNTTC 6 cut(s) 1218, 2604, 2921, 3009, 3200, 3633
PflFI GACNNNGTC 2 cut(s) 194, 446
PinAI ACCGGT 1 cut(s) 461
Ple19I CGATCG 1 cut(s) 78
PleI GAGTC 7 cut(s) 903, 1474, 2746, 2785, 4334, 4378, 4399
PluTI GGCGCC 1 cut(s) 486
PpsI GAGTC 7 cut(s) 903, 1474, 2746, 2785, 4334, 4378, 4399
PpuMI RGGWCCY 2 cut(s) 775, 2322
PsiI TTATAA 4 cut(s) 1700, 2165, 2550, 2763
Psp124BI GAGCTC 3 cut(s) 2058, 2534, 3189
Psp5II RGGWCCY 2 cut(s) 775, 2322
Psp6I CCWGG 4 cut(s) 375, 1993, 2119, 2465
PspFI CCCAGC 2 cut(s) 824, 3248
PspGI CCWGG 4 cut(s) 375, 1993, 2119, 2465
PspPI GGNCC 8 cut(s) 126, 196, 630, 775, 1420, 1659, 1809, 2322
PspPPI RGGWCCY 2 cut(s) 775, 2322
PstI CTGCAG 2 cut(s) 30, 2085
PstNI CAGNNNCTG 1 cut(s) 3293
PsuI RGATCY 8 cut(s) 143, 234, 280, 1252, 2077, 2614, 2809, 3460
PsyI GACNNNGTC 2 cut(s) 194, 446
PvuI CGATCG 1 cut(s) 78
PvuII CAGCTG 4 cut(s) 106, 3962, 4280, 4307
RseI CAYNNNNRTG 5 cut(s) 1428, 1728, 2226, 2258, 2352
SacI GAGCTC 3 cut(s) 2058, 2534, 3189
SalI GTCGAC 1 cut(s) 317
SapI GCTCTTC 2 cut(s) 92, 4279
Sau96I GGNCC 8 cut(s) 126, 196, 630, 775, 1420, 1659, 1809, 2322
ScaI AGTACT 3 cut(s) 1843, 2248, 2851
SchI GAGTC 7 cut(s) 904, 1474, 2746, 2786, 4334, 4379, 4400
ScrFI CCNGG 7 cut(s) 222, 377, 1418, 1995, 2121, 2467, 4147
SduI GDGCHC 5 cut(s) 114, 2058, 2359, 2534, 3189
SexAI ACCWGGT 1 cut(s) 2465
SfaNI GCATC 5 cut(s) 339, 722, 1303, 1588, 4342
SfcI CTRYAG 2 cut(s) 26, 2081
SfoI GGCGCC 1 cut(s) 484
SgrAI CRCCGGYG 1 cut(s) 461
SinI GGWCC 6 cut(s) 196, 630, 775, 1420, 1809, 2322
SmiMI CAYNNNNRTG 5 cut(s) 1428, 1728, 2226, 2258, 2352
SmlI CTYRAG 9 cut(s) 299, 1040, 1786, 2754, 2787, 3014, 3031, 3080, 3182
SmoI CTYRAG 9 cut(s) 299, 1040, 1786, 2754, 2787, 3014, 3031, 3080, 3182
SpeI ACTAGT 1 cut(s) 425
SphI GCATGC 1 cut(s) 2688
SspDI GGCGCC 1 cut(s) 482
SspI AATATT 1 cut(s) 1513
SspMI CTAG 5 cut(s) 426, 1877, 2864, 2937, 4290
SstI GAGCTC 3 cut(s) 2058, 2534, 3189
StyD4I CCNGG 7 cut(s) 220, 375, 1416, 1993, 2119, 2465, 4145
StyI CCWWGG 7 cut(s) 288, 851, 2070, 2224, 2618, 3124, 3342
TaiI ACGT 3 cut(s) 219, 318, 3656
TaqII GACCGA 1 cut(s) 401
TatI WGTACW 5 cut(s) 552, 1841, 2031, 2246, 2849
TauI GCSGC 5 cut(s) 56, 263, 544, 4028, 4067
TscAI CASTG 3 cut(s) 340, 2041, 3004
TseFI GTSAC 4 cut(s) 304, 637, 976, 4337
Tsp45I GTSAC 4 cut(s) 304, 637, 976, 4337
TspGWI ACGGA 5 cut(s) 196, 273, 369, 2732, 3718
TspRI CASTG 3 cut(s) 340, 2041, 3004
Tth111I GACNNNGTC 2 cut(s) 194, 446
Vha464I CTTAAG 1 cut(s) 3031
VpaK11BI GGWCC 6 cut(s) 196, 630, 775, 1420, 1809, 2322
XagI CCTNNNNNAGG 1 cut(s) 2152
XapI RAATTY 9 cut(s) 512, 662, 1234, 2724, 2768, 2859, 2921, 3009, 3041
XbaI TCTAGA 1 cut(s) 1876
XceI RCATGY 2 cut(s) 2688, 2959
XcmI CCANNNNNNNNNTGG 4 cut(s) 2616, 2745, 3447, 3667
XmiI GTMKAC 1 cut(s) 318
XmnI GAANNNNTTC 6 cut(s) 1218, 2604, 2921, 3009, 3200, 3633
XspI CTAG 5 cut(s) 426, 1877, 2864, 2937, 4290
ZraI GACGTC 1 cut(s) 316
ZrmI AGTACT 3 cut(s) 1843, 2248, 2851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.