Rmu_sc0023311.1_g000001

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0023311.1
OG0001532
Physical Location & Seq
Forward (+)
1 .. 1659
1659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0023311.1_g000001.1.cds

Sequence Viewer

Length: 1038 bp
gagagggttcaagctttattggctgatagggataaggtcaatgagcaggttactgatttgagaggttcaactccaaagtcattgtggttgaaggatcttgatgctcttgaattggaacttgatgagctggagaaaagtgaggctctggcagatgaggcaaaaaggaaatcaagaaacaaccaaatcaagaatgcgcctgttgggaaggtttctaaacaagcacctaagccacgaaagaatactaaaaaggctaacaatgcagaagctattgcagacagtagggaaacatcttccagttctgcaatggaaatggagaaagctcctgaggtggtgaaacggagaggtgcagcggcaggttcaaagaaagctcctgttaagaagcaagcaaaatccacagtagttttgtctgatgatgaagatgaagaaatgcttgatttgaaagatcgactggctgcctataatcttgattcatctccagacaaggaaactgaagtgccactaaaagcagcccagaagaaagcacctaccaaaaaggccacaataacagatgatgaggacgatgacgatgatgattttgaagtggaaatagcagctgcccccgcagcagttaagaagggaggtagaaagcctgctggagctacaaaggcagcagcagcgaagaagccccctgtggcagccaagaagagaggtccagcggctaaggagactcaaacatcatcctcttcaggccagaagctcttaaccgatatgttgaagcctgctgctgagagttctggggtttcaccagacccaaaagtgagaaagatgagggaatctccattcaacaagaagagtggttctgtgttgagccgggttaacttggaagatgagatgaacgaaagtgaagagaaaaccgaagctccagtagcagctggaagagctagaccgcagagggcggctgctcgtaacaggacaatgattctgtcaagtgattctgaggatgcaaatgatagtgatgagtctgaggtggaggaaattgattctgacgattctgagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 map01524
Pfam Domains
Protein Families
DNA_gyraseB DNA_topoisoIV HATPase_c TOPRIM_C Toprim

Protein Analysis

345

Amino Acids

37.22

Weight (kDa)

5.33

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 37, 344
AciI CCGC 5 cut(s) 350, 600, 695, 926, 935
AclWI GGATC 1 cut(s) 102
AcuI CTGAAG 2 cut(s) 510, 708
AgsI TTSAA 9 cut(s) 11, 69, 91, 110, 360, 439, 578, 754, 823
Alw26I GTCTC 1 cut(s) 698
AlwI GGATC 1 cut(s) 102
AoxI GGCC 2 cut(s) 534, 727
AspLEI GCGC 1 cut(s) 196
AspS9I GGNCC 1 cut(s) 689
AsuC2I CCSGG 1 cut(s) 851
AsuHPI GGTGA 2 cut(s) 343, 774
AvaII GGWCC 1 cut(s) 689
AxyI CCTNAGG 1 cut(s) 324
BcnI CCSGG 1 cut(s) 851
BcoDI GTCTC 1 cut(s) 698
BfaI CTAG 1 cut(s) 921
BfuAI ACCTGC 2 cut(s) 37, 344
Bme1390I CCNGG 1 cut(s) 851
Bme18I GGWCC 1 cut(s) 689
BmgT120I GGNCC 1 cut(s) 689
BmrFI CCNGG 1 cut(s) 851
BmsI GCATC 2 cut(s) 91, 970
BplI GAGNNNNNCTC 2 cut(s) 799, 831
BpmI CTGGAG 4 cut(s) 149, 459, 654, 885
Bpu10I CCTNAGC 2 cut(s) 225, 699
BpuMI CCSGG 1 cut(s) 851
BsaXI ACNNNNNCTCC 2 cut(s) 883, 913
Bse1I ACTGG 3 cut(s) 294, 453, 902
Bse21I CCTNAGG 1 cut(s) 324
Bse3DI GCAATG 1 cut(s) 309
BseGI GGATG 2 cut(s) 716, 985
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 5 cut(s) 315, 756, 966, 993, 1023
BseNI ACTGG 3 cut(s) 294, 453, 902
BsgI GTGCAG 1 cut(s) 366
BshFI GGCC 2 cut(s) 536, 729
BsiSI CCGG 1 cut(s) 850
BsmAI GTCTC 1 cut(s) 698
BsmI GAATGC 1 cut(s) 196
BsnI GGCC 2 cut(s) 536, 729
Bsp143I GATC 2 cut(s) 94, 442
BspACI CCGC 5 cut(s) 350, 600, 695, 926, 935
BspANI GGCC 2 cut(s) 536, 729
BspCNI CTCAG 5 cut(s) 316, 757, 967, 994, 1024
BspMI ACCTGC 2 cut(s) 37, 344
BspPI GGATC 1 cut(s) 102
BspQI GCTCTTC 1 cut(s) 910
BsrDI GCAATG 1 cut(s) 309
BsrI ACTGG 3 cut(s) 294, 453, 902
BssMI GATC 2 cut(s) 94, 442
Bst4CI ACNGT 2 cut(s) 278, 397
Bst6I CTCTTC 5 cut(s) 677, 727, 824, 879, 910
BstC8I GCNNGC 3 cut(s) 384, 630, 759
BstDEI CTNAG 7 cut(s) 225, 324, 699, 765, 975, 1002, 1032
BstF5I GGATG 2 cut(s) 716, 985
BstHHI GCGC 1 cut(s) 196
BstKTI GATC 2 cut(s) 97, 445
BstMAI GTCTC 1 cut(s) 698
BstMBI GATC 2 cut(s) 94, 442
BstMWI GCNNNNNNNGC 9 cut(s) 20, 155, 257, 599, 602, 644, 653, 905, 917
BstSCI CCNGG 1 cut(s) 849
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
Bsu36I CCTNAGG 1 cut(s) 324
BsuRI GGCC 2 cut(s) 536, 729
BtsCI GGATG 2 cut(s) 716, 985
BveI ACCTGC 2 cut(s) 37, 344
Cac8I GCNNGC 3 cut(s) 384, 630, 759
CfoI GCGC 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 689
CspCI CAANNNNNGTGG 2 cut(s) 814, 849
DdeI CTNAG 7 cut(s) 225, 324, 699, 765, 975, 1002, 1032
DpnI GATC 2 cut(s) 96, 444
DpnII GATC 2 cut(s) 94, 442
Eam1104I CTCTTC 5 cut(s) 677, 727, 824, 879, 910
EarI CTCTTC 5 cut(s) 677, 727, 824, 879, 910
Eco47I GGWCC 1 cut(s) 689
Eco57I CTGAAG 2 cut(s) 510, 708
Eco81I CCTNAGG 1 cut(s) 324
FaiI YATR 2 cut(s) 459, 749
FalI AAGNNNNNCTT 2 cut(s) 414, 446
FauI CCCGC 1 cut(s) 607
FokI GGATG 2 cut(s) 703, 992
FspBI CTAG 1 cut(s) 921
GlaI GCGC 1 cut(s) 195
GsuI CTGGAG 4 cut(s) 149, 459, 654, 885
HaeIII GGCC 2 cut(s) 536, 729
HapII CCGG 1 cut(s) 850
HhaI GCGC 1 cut(s) 196
Hin6I GCGC 1 cut(s) 194
HinP1I GCGC 1 cut(s) 194
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 8 cut(s) 467, 706, 812, 958, 971, 998, 1019, 1028
HpaI GTTAAC 1 cut(s) 856
HpaII CCGG 1 cut(s) 850
HphI GGTGA 2 cut(s) 343, 774
Hpy166II GTNNAC 1 cut(s) 856
Hpy188I TCNGA 5 cut(s) 409, 976, 1003, 1024, 1033
Hpy188III TCNNGA 7 cut(s) 98, 107, 171, 187, 323, 464, 476
Hpy8I GTNNAC 1 cut(s) 856
HpyAV CCTTC 3 cut(s) 85, 199, 607
HpyCH4III ACNGT 2 cut(s) 278, 397
HpyCH4V TGCA 5 cut(s) 260, 272, 302, 347, 983
HpyF10VI GCNNNNNNNGC 9 cut(s) 20, 155, 257, 599, 602, 644, 653, 905, 917
HpyF3I CTNAG 7 cut(s) 225, 324, 699, 765, 975, 1002, 1032
HspAI GCGC 1 cut(s) 194
KspAI GTTAAC 1 cut(s) 856
Kzo9I GATC 2 cut(s) 94, 442
LguI GCTCTTC 1 cut(s) 910
LmnI GCTCC 4 cut(s) 325, 373, 635, 904
LweI GCATC 2 cut(s) 91, 970
MaeI CTAG 1 cut(s) 921
MaeIII GTNAC 2 cut(s) 49, 944
MalI GATC 2 cut(s) 96, 444
MboI GATC 2 cut(s) 94, 442
MflI RGATCY 1 cut(s) 94
MluCI AATT 2 cut(s) 110, 1014
MlyI GAGTC 2 cut(s) 700, 1007
MseI TTAA 4 cut(s) 375, 609, 740, 855
MspA1I CMGCKG 4 cut(s) 350, 593, 695, 911
MspI CCGG 1 cut(s) 850
MspR9I CCNGG 1 cut(s) 851
Mva1269I GAATGC 1 cut(s) 196
MwoI GCNNNNNNNGC 9 cut(s) 20, 155, 257, 599, 602, 644, 653, 905, 917
NciI CCSGG 1 cut(s) 851
NdeII GATC 2 cut(s) 94, 442
PciSI GCTCTTC 1 cut(s) 910
PctI GAATGC 1 cut(s) 196
PfeI GAWTC 6 cut(s) 467, 812, 958, 971, 1019, 1028
PleI GAGTC 2 cut(s) 700, 1006
PpsI GAGTC 2 cut(s) 700, 1006
PspPI GGNCC 1 cut(s) 689
PsuI RGATCY 1 cut(s) 94
PvuII CAGCTG 2 cut(s) 593, 911
SapI GCTCTTC 1 cut(s) 910
SaqAI TTAA 4 cut(s) 375, 609, 740, 855
Sau3AI GATC 2 cut(s) 94, 442
Sau96I GGNCC 1 cut(s) 689
SchI GAGTC 2 cut(s) 700, 1007
ScrFI CCNGG 1 cut(s) 851
SfaNI GCATC 2 cut(s) 91, 970
SinI GGWCC 1 cut(s) 689
Sse9I AATT 2 cut(s) 110, 1014
SsiI CCGC 5 cut(s) 350, 600, 695, 926, 935
SspMI CTAG 1 cut(s) 921
StyD4I CCNGG 1 cut(s) 849
TaaI ACNGT 2 cut(s) 278, 397
TaqI TCGA 1 cut(s) 445
TasI AATT 2 cut(s) 110, 1014
TauI GCSGC 3 cut(s) 353, 698, 938
TfiI GAWTC 6 cut(s) 467, 812, 958, 971, 1019, 1028
Tru1I TTAA 4 cut(s) 375, 609, 740, 855
Tru9I TTAA 4 cut(s) 375, 609, 740, 855
TspDTI ATGAA 4 cut(s) 429, 435, 459, 887
TspGWI ACGGA 1 cut(s) 352
VpaK11BI GGWCC 1 cut(s) 689
XcmI CCANNNNNNNNNTGG 2 cut(s) 81, 301
XspI CTAG 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.