Rorug01G0387200

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001532
Physical Location & Seq
Forward (+)
49866948 .. 49868874
1927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0387200.1

Sequence Viewer

Length: 1461 bp
ATGCCTTCTTCTTCGCCAATCTCTCTCACCGTGGTGTCTGAATCCATGGTGTTTCCTGACCAGACCTCCACCCTCGGAGACCTCAAGCTTTCGGTTTCTGACCTCCCCATGCTCTCATGTCACTACATCCAAAAGGGAGGCCTCTTCACTAAGCCTTCTTTCCCAATCCAATCCCTAGTCTCGCTACTCAAGACCGGCCTCTCCCAGACCCTCACCCGCTTCCCTCCCTTGGCCGGCCGGTTGATCACAGACAAAGATGGTTACATATACATCACCTGCAACGACGCCGGTGTCGATTTTATTCACGCAGCGGCGACTGAGGTCTCCGTGCGTGACATTCTGTCGCCGACTCACGTGCCGGAGTCGGTGAAGGAGTTTTTTGCATTGGACAGGACTGTGAGCTACACCGGCCATCACAACCCAATCCTGGCAGTCCAGGTCACCGAGCTCAAAGACGGCGTTTTCATCGGTTGCGCCGTCAACCACGCCGTCATTGACGGCACCTCCTTCTGGAACTTCTTCAACACCTTCTCCGAGGTCTGCCGCGGCGGCAAGAGCTTCTCCAGAAACCCGGACTTCACCCGGGACTCGATTCTGATTTCTCAGGCTGTCCTTCAGTTGCCGGAAGGTGGCCCCAAGGTCTCATTCAACGTCAACGAGCCGTTGACCGAGAGAATCTTCAGCTTCAGCAGAGAAGCCATTCTGAAACTAAAAGCCACAACTAACAACAACAAATGGACCCAAAACGACGACGTCGCCACCGCCGTCGAGATTCTCGGGAAGCAGAGCAACGACCCTTACGAAACTAACAACAACAACAACAACGCCACTGTAACCGCCATCATCGAAAGCTGGTTCAAGAATTCCAAGCCGCAAATCACTGAGAACAAAACGGCAGCGGAGATCTCGTCGTTTCAGTCGCTCTGCGCGCTTCTGTGGAAGGCTGTGACACGCGCCAGGAAGCTACCGGAGTCGAAAACGACGACGTTCAGAATGGCAGTGAACTGCCGTCACAGGCTGGAGCCGAAGCTGGACCCGTACTACTTCGGGAACGCAATCCAAAGCATCCCGACGTACGCGTCCTCGGGAGACGTGTTGTCTAGGGATCTGAGATGGTGCGCGGAGCAGCTCAACGACAACGTGAAGGCGCACAATAGCGATAGGGTAAGGGAGTGCGTAGAGGATTGGGAGAAGAACCCGCGGGTGTTTCCGCTAGGGAACTTCGACGGTGCATCGATGACGATGGGTAGCTCGCCGAGGTTTCCGATGTACGACAACGATTTCGGGTGGGGCAGGCCGTTGGCGGTTCGGAGCGGGCCGGCGAATAAATTCGACGGTAAGATTTCGGCGTTTCCGGGGAGGGACGGAGCTGGGACCGTTGATCTGGAGGTGGTTTTGGCGCCTGACACGATGGCGGGGTTGGAGTCTGATTTGGAGTTCATGCAATATGTGTCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 map01524
Pfam Domains
Protein Families
DNA_gyraseB DNA_topoisoIV HATPase_c TOPRIM_C Toprim

Protein Analysis

486

Amino Acids

53.69

Weight (kDa)

5.72

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 8 - 481 3.9e-114 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 284
AasI GACNNNNNNGTC 2 cut(s) 290, 1078
AatII GACGTC 1 cut(s) 756
Acc36I ACCTGC 1 cut(s) 284
AccB1I GGYRCC 2 cut(s) 500, 1399
AccBSI CCGCTC 1 cut(s) 1314
AccII CGCG 6 cut(s) 546, 929, 954, 1079, 1121, 1201
AclWI GGATC 1 cut(s) 1113
AcoI YGGCCR 3 cut(s) 231, 235, 409
AcsI RAATTY 2 cut(s) 862, 1328
AcuI CTGAAG 3 cut(s) 599, 664, 670
AcvI CACGTG 1 cut(s) 355
AcyI GRCGYC 3 cut(s) 285, 753, 1400
AfaI GTAC 3 cut(s) 1040, 1076, 1271
AfiI CCNNNNNNNGG 5 cut(s) 229, 233, 427, 510, 629
AflIII ACRYGT 2 cut(s) 1077, 1092
AgsI TTSAA 3 cut(s) 523, 649, 859
AhdI GACNNNNNGTC 2 cut(s) 340, 1096
AjiI CACGTC 1 cut(s) 1093
AjnI CCWGG 3 cut(s) 426, 435, 956
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AleI CACNNNNGTG 1 cut(s) 32
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 5 cut(s) 72, 184, 328, 646, 1083
AlwI GGATC 1 cut(s) 1113
Ama87I CYCGRG 3 cut(s) 582, 776, 1084
AoxI GGCC 8 cut(s) 139, 196, 231, 235, 409, 631, 1295, 1316
ApeKI GCWGC 3 cut(s) 308, 896, 1126
ApoI RAATTY 2 cut(s) 862, 1328
ArsI GACNNNNNNTTYG 2 cut(s) 1265, 1297
Asp700I GAANNNNTTC 3 cut(s) 518, 699, 1328
AspLEI GCGC 7 cut(s) 476, 929, 931, 956, 1121, 1150, 1402
AspS9I GGNCC 5 cut(s) 632, 738, 1033, 1316, 1374
AsuC2I CCSGG 4 cut(s) 572, 583, 584, 1356
AsuHPI GGTGA 6 cut(s) 19, 205, 265, 379, 433, 571
AvaI CYCGRG 3 cut(s) 582, 776, 1084
AvaII GGWCC 3 cut(s) 738, 1033, 1374
BanI GGYRCC 2 cut(s) 500, 1399
BanII GRGCYC 1 cut(s) 450
BbrPI CACGTG 1 cut(s) 355
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 3 cut(s) 320, 908, 1138
BccI CCATC 6 cut(s) 251, 420, 848, 1107, 1237, 1405
BceAI ACGGC 9 cut(s) 461, 472, 473, 514, 646, 749, 909, 993, 1282
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BciT130I CCWGG 3 cut(s) 428, 437, 958
BclI TGATCA 1 cut(s) 243
BcnI CCSGG 4 cut(s) 572, 583, 584, 1356
BcoDI GTCTC 5 cut(s) 72, 184, 328, 646, 1083
BfaI CTAG 3 cut(s) 176, 1101, 1214
BfoI RGCGCY 1 cut(s) 1403
BfuAI ACCTGC 1 cut(s) 284
BglI GCCNNNNNGGC 1 cut(s) 549
BglII AGATCT 1 cut(s) 903
BisI GCNGC 8 cut(s) 309, 312, 544, 547, 550, 872, 897, 1127
BlsI GCNGC 8 cut(s) 310, 313, 545, 548, 551, 873, 898, 1128
Bme1390I CCNGG 7 cut(s) 428, 437, 572, 583, 584, 958, 1356
Bme18I GGWCC 3 cut(s) 738, 1033, 1374
BmeRI GACNNNNNGTC 2 cut(s) 340, 1096
BmeT110I CYCGRG 3 cut(s) 582, 776, 1084
BmgBI CACGTC 1 cut(s) 1093
BmgT120I GGNCC 5 cut(s) 632, 738, 1033, 1316, 1374
BmiI GGNNCC 7 cut(s) 502, 634, 740, 1023, 1035, 1375, 1401
BmrFI CCNGG 7 cut(s) 428, 437, 572, 583, 584, 958, 1356
BmsI GCATC 2 cut(s) 1074, 1241
BoxI GACNNNNGTC 1 cut(s) 320
BpmI CTGGAG 3 cut(s) 547, 1040, 1406
BpuEI CTTGAG 2 cut(s) 68, 173
BpuMI CCSGG 4 cut(s) 572, 583, 584, 1356
Bsa29I ATCGAT 1 cut(s) 1235
BsaAI YACGTR 1 cut(s) 355
BsaHI GRCGYC 3 cut(s) 285, 753, 1400
BsaI GGTCTC 3 cut(s) 72, 328, 646
BsaWI WCCGGW 1 cut(s) 967
BsaXI ACNNNNNCTCC 8 cut(s) 50, 80, 488, 515, 518, 545, 893, 923
Bsc4I CCNNNNNNNGG 5 cut(s) 229, 233, 427, 510, 629
Bse118I RCCGGY 6 cut(s) 194, 233, 237, 287, 407, 1318
BseBI CCWGG 3 cut(s) 428, 437, 958
BseCI ATCGAT 1 cut(s) 1235
BseGI GGATG 2 cut(s) 126, 1065
BseLI CCNNNNNNNGG 5 cut(s) 229, 233, 427, 510, 629
BseMII CTCAG 4 cut(s) 309, 617, 873, 1100
BsePI GCGCGC 1 cut(s) 927
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 3 cut(s) 320, 908, 1138
BseYI CCCAGC 1 cut(s) 1370
Bsh1236I CGCG 6 cut(s) 546, 929, 954, 1079, 1121, 1201
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 8 cut(s) 141, 198, 233, 237, 411, 633, 1297, 1318
BshNI GGYRCC 2 cut(s) 500, 1399
BshVI ATCGAT 1 cut(s) 1235
BsiEI CGRYCG 1 cut(s) 238
BsiHKAI GWGCWC 1 cut(s) 450
BsiHKCI CYCGRG 3 cut(s) 582, 776, 1084
BsiWI CGTACG 1 cut(s) 1074
BslFI GGGAC 3 cut(s) 599, 1376, 1387
BslI CCNNNNNNNGG 5 cut(s) 229, 233, 427, 510, 629
BsmAI GTCTC 5 cut(s) 72, 184, 328, 646, 1083
BsmBI CGTCTC 1 cut(s) 1083
BsmFI GGGAC 3 cut(s) 599, 1376, 1387
BsnI GGCC 8 cut(s) 141, 198, 233, 237, 411, 633, 1297, 1318
Bso31I GGTCTC 3 cut(s) 72, 328, 646
BsoBI CYCGRG 3 cut(s) 582, 776, 1084
Bsp1286I GDGCHC 1 cut(s) 450
Bsp143I GATC 4 cut(s) 243, 903, 1105, 1381
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 8 cut(s) 141, 198, 233, 237, 411, 633, 1297, 1318
BspCNI CTCAG 4 cut(s) 310, 616, 874, 1101
BspDI ATCGAT 1 cut(s) 1235
BspFNI CGCG 6 cut(s) 546, 929, 954, 1079, 1121, 1201
BspLI GGNNCC 7 cut(s) 502, 634, 740, 1023, 1035, 1375, 1401
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 1 cut(s) 1113
BspT107I GGYRCC 2 cut(s) 500, 1399
BspTNI GGTCTC 3 cut(s) 72, 328, 646
BsrBI CCGCTC 1 cut(s) 1314
BsrFI RCCGGY 6 cut(s) 194, 233, 237, 287, 407, 1318
BssAI RCCGGY 6 cut(s) 194, 233, 237, 287, 407, 1318
BssHII GCGCGC 1 cut(s) 927
BssMI GATC 4 cut(s) 243, 903, 1105, 1381
BssNI GRCGYC 3 cut(s) 285, 753, 1400
BssT1I CCWWGG 3 cut(s) 45, 228, 636
Bst2UI CCWGG 3 cut(s) 428, 437, 958
Bst4CI ACNGT 6 cut(s) 31, 397, 832, 1229, 1337, 1378
Bst6I CTCTTC 1 cut(s) 149
BstACI GRCGYC 3 cut(s) 285, 753, 1400
BstBAI YACGTR 1 cut(s) 355
BstC8I GCNNGC 6 cut(s) 235, 929, 1253, 1295, 1316, 1320
BstDEI CTNAG 5 cut(s) 150, 318, 603, 882, 1109
BstDSI CCRYGG 4 cut(s) 30, 45, 544, 1199
BstEII GGTNACC 1 cut(s) 439
BstF5I GGATG 2 cut(s) 126, 1065
BstFNI CGCG 6 cut(s) 546, 929, 954, 1079, 1121, 1201
BstH2I RGCGCY 1 cut(s) 1403
BstHHI GCGC 7 cut(s) 476, 929, 931, 956, 1121, 1150, 1402
BstKTI GATC 4 cut(s) 246, 906, 1108, 1384
BstMAI GTCTC 5 cut(s) 72, 184, 328, 646, 1083
BstMBI GATC 4 cut(s) 243, 903, 1105, 1381
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 4 cut(s) 408, 549, 555, 928
BstNI CCWGG 3 cut(s) 428, 437, 958
BstPAI GACNNNNGTC 1 cut(s) 320
BstPI GGTNACC 1 cut(s) 439
BstSCI CCNGG 7 cut(s) 426, 435, 570, 581, 582, 956, 1354
BstUI CGCG 6 cut(s) 546, 929, 954, 1079, 1121, 1201
BstV1I GCAGC 3 cut(s) 320, 908, 1138
BstX2I RGATCY 2 cut(s) 903, 1105
BstYI RGATCY 2 cut(s) 903, 1105
BstZI CGGCCG 1 cut(s) 235
Bsu15I ATCGAT 1 cut(s) 1235
BsuRI GGCC 8 cut(s) 141, 198, 233, 237, 411, 633, 1297, 1318
BsuTUI ATCGAT 1 cut(s) 1235
BtgI CCRYGG 4 cut(s) 30, 45, 544, 1199
BtrI CACGTC 1 cut(s) 1093
BtsCI GGATG 2 cut(s) 126, 1065
BtsI GCAGTG 1 cut(s) 1005
BtsIMutI CAGTG 3 cut(s) 828, 879, 1005
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 6 cut(s) 235, 929, 1253, 1295, 1316, 1320
CfoI GCGC 7 cut(s) 476, 929, 931, 956, 1121, 1150, 1402
Cfr10I RCCGGY 6 cut(s) 194, 233, 237, 287, 407, 1318
Cfr13I GGNCC 5 cut(s) 632, 738, 1033, 1316, 1374
Cfr42I CCGCGG 2 cut(s) 547, 1202
Cfr9I CCCGGG 1 cut(s) 582
ClaI ATCGAT 1 cut(s) 1235
CseI GACGC 2 cut(s) 293, 1068
Csp6I GTAC 3 cut(s) 1039, 1075, 1270
CviAII CATG 4 cut(s) 46, 109, 117, 1441
CviQI GTAC 3 cut(s) 1039, 1075, 1270
DdeI CTNAG 5 cut(s) 150, 318, 603, 882, 1109
DinI GGCGCC 1 cut(s) 1401
DpnI GATC 4 cut(s) 245, 905, 1107, 1383
DpnII GATC 4 cut(s) 243, 903, 1105, 1381
DrdI GACNNNNNNGTC 2 cut(s) 290, 1078
DriI GACNNNNNGTC 2 cut(s) 340, 1096
DseDI GACNNNNNNGTC 2 cut(s) 290, 1078
EaeI YGGCCR 3 cut(s) 231, 235, 409
EagI CGGCCG 1 cut(s) 235
Eam1104I CTCTTC 1 cut(s) 149
Eam1105I GACNNNNNGTC 2 cut(s) 340, 1096
EarI CTCTTC 1 cut(s) 149
Ecl136II GAGCTC 1 cut(s) 448
EclXI CGGCCG 1 cut(s) 235
Eco130I CCWWGG 3 cut(s) 45, 228, 636
Eco147I AGGCCT 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 450
Eco31I GGTCTC 3 cut(s) 72, 328, 646
Eco47I GGWCC 3 cut(s) 738, 1033, 1374
Eco52I CGGCCG 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 448
Eco57I CTGAAG 3 cut(s) 599, 664, 670
Eco72I CACGTG 1 cut(s) 355
Eco88I CYCGRG 3 cut(s) 582, 776, 1084
Eco91I GGTNACC 1 cut(s) 439
EcoICRI GAGCTC 1 cut(s) 448
EcoO65I GGTNACC 1 cut(s) 439
EcoRI GAATTC 1 cut(s) 862
EcoRII CCWGG 3 cut(s) 426, 435, 956
EcoT14I CCWWGG 3 cut(s) 45, 228, 636
EcoT38I GRGCYC 1 cut(s) 450
EgeI GGCGCC 1 cut(s) 1401
EheI GGCGCC 1 cut(s) 1401
ErhI CCWWGG 3 cut(s) 45, 228, 636
Esp3I CGTCTC 1 cut(s) 1083
FaeI CATG 4 cut(s) 49, 112, 120, 1444
FaiI YATR 7 cut(s) 47, 110, 118, 266, 268, 1442, 1449
FaqI GGGAC 3 cut(s) 599, 1376, 1387
FatI CATG 4 cut(s) 45, 108, 116, 1440
FauI CCCGC 5 cut(s) 224, 1194, 1206, 1307, 1408
FbaI TGATCA 1 cut(s) 243
Fnu4HI GCNGC 8 cut(s) 309, 312, 544, 547, 550, 872, 897, 1127
FokI GGATG 2 cut(s) 113, 1052
FriOI GRGCYC 1 cut(s) 450
FseI GGCCGGCC 1 cut(s) 237
Fsp4HI GCNGC 8 cut(s) 309, 312, 544, 547, 550, 872, 897, 1127
FspBI CTAG 3 cut(s) 176, 1101, 1214
GlaI GCGC 7 cut(s) 475, 928, 930, 955, 1120, 1149, 1401
GluI GCNGC 8 cut(s) 309, 312, 544, 547, 550, 872, 897, 1127
GsaI CCCAGC 1 cut(s) 1374
GsuI CTGGAG 3 cut(s) 547, 1040, 1406
HaeII RGCGCY 1 cut(s) 1403
HaeIII GGCC 8 cut(s) 141, 198, 233, 237, 411, 633, 1297, 1318
HgaI GACGC 2 cut(s) 293, 1068
HhaI GCGC 7 cut(s) 476, 929, 931, 956, 1121, 1150, 1402
Hin1I GRCGYC 3 cut(s) 285, 753, 1400
Hin1II CATG 4 cut(s) 49, 112, 120, 1444
Hin6I GCGC 7 cut(s) 474, 927, 929, 954, 1119, 1148, 1400
HinP1I GCGC 7 cut(s) 474, 927, 929, 954, 1119, 1148, 1400
HincII GTYRAC 3 cut(s) 481, 655, 666
HindII GTYRAC 3 cut(s) 481, 655, 666
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 9 cut(s) 41, 349, 362, 587, 592, 675, 772, 971, 1424
HphI GGTGA 6 cut(s) 19, 205, 265, 379, 433, 571
Hpy166II GTNNAC 4 cut(s) 481, 655, 666, 1003
Hpy8I GTNNAC 4 cut(s) 481, 655, 666, 1003
HpyAV CCTTC 9 cut(s) 15, 165, 364, 517, 538, 620, 623, 934, 1138
HpyCH4III ACNGT 6 cut(s) 31, 397, 832, 1229, 1337, 1378
HpyCH4IV ACGT 7 cut(s) 354, 651, 753, 986, 1073, 1092, 1140
HpyCH4V TGCA 4 cut(s) 279, 383, 1232, 1444
HpyF10VI GCNNNNNNNGC 4 cut(s) 408, 549, 555, 928
HpyF3I CTNAG 5 cut(s) 150, 318, 603, 882, 1109
HpySE526I ACGT 7 cut(s) 354, 651, 753, 986, 1073, 1092, 1140
Hsp92I GRCGYC 3 cut(s) 285, 753, 1400
Hsp92II CATG 4 cut(s) 49, 112, 120, 1444
HspAI GCGC 7 cut(s) 474, 927, 929, 954, 1119, 1148, 1400
KasI GGCGCC 1 cut(s) 1399
KroI GCCGGC 2 cut(s) 233, 1318
KroNI GCCGGC 2 cut(s) 235, 1320
Ksp22I TGATCA 1 cut(s) 243
KspI CCGCGG 2 cut(s) 547, 1202
Kzo9I GATC 4 cut(s) 243, 903, 1105, 1381
LmnI GCTCC 4 cut(s) 1021, 1123, 1311, 1367
Lsp1109I GCAGC 3 cut(s) 320, 908, 1138
LweI GCATC 2 cut(s) 1074, 1241
MaeI CTAG 3 cut(s) 176, 1101, 1214
MaeII ACGT 7 cut(s) 354, 651, 753, 986, 1073, 1092, 1140
MaeIII GTNAC 7 cut(s) 119, 260, 332, 439, 832, 946, 1010
MalI GATC 4 cut(s) 245, 905, 1107, 1383
MbiI CCGCTC 1 cut(s) 1314
MboI GATC 4 cut(s) 243, 903, 1105, 1381
MboII GAAGA 5 cut(s) 3, 136, 511, 670, 1204
MflI RGATCY 2 cut(s) 903, 1105
MhlI GDGCHC 1 cut(s) 450
MluCI AATT 2 cut(s) 862, 1328
MluI ACGCGT 1 cut(s) 1077
Mly113I GGCGCC 1 cut(s) 1400
MlyI GAGTC 5 cut(s) 343, 371, 581, 980, 1433
MmeI TCCRAC 1 cut(s) 1401
MroNI GCCGGC 2 cut(s) 233, 1318
MroXI GAANNNNTTC 3 cut(s) 518, 699, 1328
MslI CAYNNNNRTG 1 cut(s) 32
MspA1I CMGCKG 4 cut(s) 311, 546, 899, 1201
MspR9I CCNGG 7 cut(s) 428, 437, 572, 583, 584, 958, 1356
MvaI CCWGG 3 cut(s) 428, 437, 958
MvnI CGCG 6 cut(s) 546, 929, 954, 1079, 1121, 1201
MwoI GCNNNNNNNGC 4 cut(s) 408, 549, 555, 928
NaeI GCCGGC 2 cut(s) 235, 1320
NarI GGCGCC 1 cut(s) 1400
NciI CCSGG 4 cut(s) 572, 583, 584, 1356
NcoI CCATGG 1 cut(s) 45
NdeII GATC 4 cut(s) 243, 903, 1105, 1381
NgoMIV GCCGGC 2 cut(s) 233, 1318
NlaIII CATG 4 cut(s) 49, 112, 120, 1444
NlaIV GGNNCC 7 cut(s) 502, 634, 740, 1023, 1035, 1375, 1401
NmeAIII GCCGAG 1 cut(s) 1281
NmuCI GTSAC 5 cut(s) 119, 332, 439, 946, 1010
OliI CACNNNNGTG 1 cut(s) 32
PaqCI CACCTGC 1 cut(s) 284
PauI GCGCGC 1 cut(s) 927
PceI AGGCCT 1 cut(s) 141
PcsI WCGNNNNNNNCGW 5 cut(s) 291, 474, 762, 798, 980
PdiI GCCGGC 2 cut(s) 235, 1320
PdmI GAANNNNTTC 3 cut(s) 518, 699, 1328
PfeI GAWTC 4 cut(s) 41, 592, 675, 772
Pfl23II CGTACG 1 cut(s) 1074
PflFI GACNNNGTC 1 cut(s) 752
PkrI GCNGC 8 cut(s) 310, 313, 545, 548, 551, 873, 898, 1128
PleI GAGTC 5 cut(s) 343, 370, 581, 979, 1432
PluTI GGCGCC 1 cut(s) 1403
PmaCI CACGTG 1 cut(s) 355
PmlI CACGTG 1 cut(s) 355
PpsI GAGTC 5 cut(s) 343, 370, 581, 979, 1432
Ppu21I YACGTR 1 cut(s) 355
PshAI GACNNNNGTC 1 cut(s) 320
Psp124BI GAGCTC 1 cut(s) 450
Psp6I CCWGG 3 cut(s) 426, 435, 956
PspCI CACGTG 1 cut(s) 355
PspEI GGTNACC 1 cut(s) 439
PspFI CCCAGC 1 cut(s) 1370
PspGI CCWGG 3 cut(s) 426, 435, 956
PspLI CGTACG 1 cut(s) 1074
PspN4I GGNNCC 7 cut(s) 502, 634, 740, 1023, 1035, 1375, 1401
PspPI GGNCC 5 cut(s) 632, 738, 1033, 1316, 1374
PsuI RGATCY 2 cut(s) 903, 1105
PsyI GACNNNGTC 1 cut(s) 752
PteI GCGCGC 1 cut(s) 927
RigI GGCCGGCC 1 cut(s) 237
RsaI GTAC 3 cut(s) 1040, 1076, 1271
RsaNI GTAC 3 cut(s) 1039, 1075, 1270
RseI CAYNNNNRTG 1 cut(s) 32
SacI GAGCTC 1 cut(s) 450
SacII CCGCGG 2 cut(s) 547, 1202
SatI GCNGC 8 cut(s) 309, 312, 544, 547, 550, 872, 897, 1127
Sau3AI GATC 4 cut(s) 243, 903, 1105, 1381
Sau96I GGNCC 5 cut(s) 632, 738, 1033, 1316, 1374
SchI GAGTC 5 cut(s) 343, 371, 581, 980, 1433
ScrFI CCNGG 7 cut(s) 428, 437, 572, 583, 584, 958, 1356
SduI GDGCHC 1 cut(s) 450
SfaNI GCATC 2 cut(s) 1074, 1241
SfoI GGCGCC 1 cut(s) 1401
Sfr303I CCGCGG 2 cut(s) 547, 1202
SgrAI CRCCGGYG 1 cut(s) 287
SgrBI CCGCGG 2 cut(s) 547, 1202
SinI GGWCC 3 cut(s) 738, 1033, 1374
SmaI CCCGGG 1 cut(s) 584
SmiMI CAYNNNNRTG 1 cut(s) 32
SmlI CTYRAG 2 cut(s) 83, 188
SmoI CTYRAG 2 cut(s) 83, 188
Sse9I AATT 2 cut(s) 862, 1328
SseBI AGGCCT 1 cut(s) 141
SspDI GGCGCC 1 cut(s) 1399
SspMI CTAG 3 cut(s) 176, 1101, 1214
SstI GAGCTC 1 cut(s) 450
StuI AGGCCT 1 cut(s) 141
StyD4I CCNGG 7 cut(s) 426, 435, 570, 581, 582, 956, 1354
StyI CCWWGG 3 cut(s) 45, 228, 636
TaaI ACNGT 6 cut(s) 31, 397, 832, 1229, 1337, 1378
TaiI ACGT 7 cut(s) 357, 654, 756, 989, 1076, 1095, 1143
TaqI TCGA 8 cut(s) 294, 590, 768, 846, 974, 1224, 1235, 1332
TaqII GACCGA 1 cut(s) 683
TasI AATT 2 cut(s) 862, 1328
TauI GCSGC 5 cut(s) 314, 546, 549, 552, 874
TfiI GAWTC 4 cut(s) 41, 592, 675, 772
TscAI CASTG 3 cut(s) 835, 886, 1005
TseFI GTSAC 5 cut(s) 119, 332, 439, 946, 1010
TseI GCWGC 3 cut(s) 308, 896, 1126
Tsp45I GTSAC 5 cut(s) 119, 332, 439, 946, 1010
TspDTI ATGAA 2 cut(s) 454, 1429
TspGWI ACGGA 2 cut(s) 316, 1380
TspMI CCCGGG 1 cut(s) 582
TspRI CASTG 3 cut(s) 835, 886, 1005
Tth111I GACNNNGTC 1 cut(s) 752
VpaK11BI GGWCC 3 cut(s) 738, 1033, 1374
XapI RAATTY 2 cut(s) 862, 1328
XmaI CCCGGG 1 cut(s) 582
XmnI GAANNNNTTC 3 cut(s) 518, 699, 1328
XspI CTAG 3 cut(s) 176, 1101, 1214
ZraI GACGTC 1 cut(s) 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.