Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 69, 795, 1008 |
| AatII | GACGTC | 1 cut(s) | 962 |
| Acc36I | ACCTGC | 2 cut(s) | 333, 1813 |
| AccB1I | GGYRCC | 1 cut(s) | 850 |
| AciI | CCGC | 5 cut(s) | 320, 1139, 1655, 2219, 2403 |
| AclWI | GGATC | 6 cut(s) | 854, 867, 944, 1049, 1062, 1713 |
| AcoI | YGGCCR | 1 cut(s) | 1140 |
| AcsI | RAATTY | 6 cut(s) | 1013, 1166, 1235, 1254, 1286, 2059 |
| AcuI | CTGAAG | 4 cut(s) | 1053, 1181, 2100, 2499 |
| AcyI | GRCGYC | 1 cut(s) | 959 |
| AfaI | GTAC | 8 cut(s) | 246, 278, 493, 568, 869, 1096, 1803, 1819 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 399, 1330, 1614, 2184, 2185, 2186 |
| AflII | CTTAAG | 1 cut(s) | 1276 |
| AflIII | ACRYGT | 1 cut(s) | 2316 |
| AjiI | CACGTC | 1 cut(s) | 90 |
| AjnI | CCWGG | 4 cut(s) | 238, 364, 863, 2342 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1043, 1075, 1298, 1330 |
| AleI | CACNNNNGTG | 2 cut(s) | 471, 1119 |
| Alw21I | GWGCWC | 4 cut(s) | 604, 779, 1434, 1558 |
| Alw26I | GTCTC | 3 cut(s) | 798, 950, 2055 |
| AlwI | GGATC | 6 cut(s) | 854, 867, 944, 1049, 1062, 1713 |
| Ama87I | CYCGRG | 3 cut(s) | 5, 799, 1019 |
| AoxI | GGCC | 5 cut(s) | 1140, 1623, 1647, 1857, 2296 |
| ApoI | RAATTY | 6 cut(s) | 1013, 1166, 1235, 1254, 1286, 2059 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 2112, 2138, 2144, 2170 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1254 |
| AspLEI | GCGC | 1 cut(s) | 2261 |
| AspS9I | GGNCC | 5 cut(s) | 54, 322, 333, 1599, 1931 |
| AsuHPI | GGTGA | 4 cut(s) | 485, 851, 2343, 2539 |
| AvaI | CYCGRG | 3 cut(s) | 5, 799, 1019 |
| AvaII | GGWCC | 5 cut(s) | 54, 322, 333, 1599, 1931 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2067, 2100 |
| BamHI | GGATCC | 2 cut(s) | 859, 1054 |
| BanI | GGYRCC | 1 cut(s) | 850 |
| BanII | GRGCYC | 2 cut(s) | 779, 1434 |
| BauI | CACGAG | 2 cut(s) | 1192, 2088 |
| BbsI | GAAGAC | 1 cut(s) | 2616 |
| Bbv12I | GWGCWC | 4 cut(s) | 604, 779, 1434, 1558 |
| BccI | CCATC | 8 cut(s) | 158, 347, 499, 683, 830, 1129, 1882, 2407 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1183, 1217, 2582, 2616 |
| BciT130I | CCWGG | 4 cut(s) | 240, 366, 865, 2344 |
| BciVI | GTATCC | 1 cut(s) | 2068 |
| BclI | TGATCA | 2 cut(s) | 253, 1087 |
| BcoDI | GTCTC | 3 cut(s) | 798, 950, 2055 |
| BfaI | CTAG | 4 cut(s) | 1478, 2138, 2514, 2580 |
| BfmI | CTRYAG | 2 cut(s) | 1098, 1947 |
| BfrI | CTTAAG | 1 cut(s) | 1276 |
| BfuAI | ACCTGC | 2 cut(s) | 333, 1813 |
| BfuI | GTATCC | 1 cut(s) | 2068 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1631 |
| BmcAI | AGTACT | 2 cut(s) | 493, 1096 |
| Bme1390I | CCNGG | 4 cut(s) | 240, 366, 865, 2344 |
| Bme18I | GGWCC | 5 cut(s) | 54, 322, 333, 1599, 1931 |
| BmeT110I | CYCGRG | 3 cut(s) | 5, 799, 1019 |
| BmgBI | CACGTC | 1 cut(s) | 90 |
| BmgT120I | GGNCC | 5 cut(s) | 54, 322, 333, 1599, 1931 |
| BmiI | GGNNCC | 5 cut(s) | 641, 852, 861, 1056, 1600 |
| BmrFI | CCNGG | 4 cut(s) | 240, 366, 865, 2344 |
| BmrI | ACTGGG | 1 cut(s) | 424 |
| BmsI | GCATC | 5 cut(s) | 832, 1702, 2234, 2390, 2590 |
| BmuI | ACTGGG | 1 cut(s) | 424 |
| BpiI | GAAGAC | 1 cut(s) | 2616 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1013, 1045 |
| BpmI | CTGGAG | 3 cut(s) | 261, 1230, 1760 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2355 |
| BpuEI | CTTGAG | 5 cut(s) | 984, 1280, 1346, 1448, 2030 |
| BsaHI | GRCGYC | 1 cut(s) | 959 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1915, 1945 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 399, 1330, 1614, 2184, 2185, 2186 |
| Bse1I | ACTGG | 5 cut(s) | 224, 430, 886, 1878, 2096 |
| Bse3DI | GCAATG | 2 cut(s) | 46, 852 |
| BseBI | CCWGG | 4 cut(s) | 240, 366, 865, 2344 |
| BseGI | GGATG | 2 cut(s) | 510, 661 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 399, 1330, 1614, 2184, 2185, 2186 |
| BseMI | GCAATG | 2 cut(s) | 46, 852 |
| BseMII | CTCAG | 8 cut(s) | 441, 979, 1524, 1899, 1973, 2325, 2346, 2534 |
| BseNI | ACTGG | 5 cut(s) | 224, 430, 886, 1878, 2096 |
| BseRI | GAGGAG | 2 cut(s) | 1139, 1778 |
| BseYI | CCCAGC | 1 cut(s) | 2236 |
| BshFI | GGCC | 5 cut(s) | 1142, 1625, 1649, 1859, 2298 |
| BshNI | GGYRCC | 1 cut(s) | 850 |
| BsiHKAI | GWGCWC | 4 cut(s) | 604, 779, 1434, 1558 |
| BsiHKCI | CYCGRG | 3 cut(s) | 5, 799, 1019 |
| BslFI | GGGAC | 2 cut(s) | 778, 1612 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 399, 1330, 1614, 2184, 2185, 2186 |
| BsmAI | GTCTC | 3 cut(s) | 798, 950, 2055 |
| BsmBI | CGTCTC | 2 cut(s) | 950, 2055 |
| BsmFI | GGGAC | 2 cut(s) | 778, 1612 |
| BsmI | GAATGC | 1 cut(s) | 454 |
| BsnI | GGCC | 5 cut(s) | 1142, 1625, 1649, 1859, 2298 |
| BsoBI | CYCGRG | 3 cut(s) | 5, 799, 1019 |
| Bsp1286I | GDGCHC | 4 cut(s) | 604, 779, 1434, 1558 |
| Bsp143I | GATC | 9 cut(s) | 253, 859, 936, 1054, 1087, 1213, 1389, 1417, 1705 |
| Bsp19I | CCATGG | 3 cut(s) | 315, 469, 1587 |
| BspACI | CCGC | 5 cut(s) | 320, 1139, 1655, 2219, 2403 |
| BspANI | GGCC | 5 cut(s) | 1142, 1625, 1649, 1859, 2298 |
| BspCNI | CTCAG | 8 cut(s) | 442, 978, 1525, 1900, 1974, 2326, 2347, 2533 |
| BspLI | GGNNCC | 5 cut(s) | 641, 852, 861, 1056, 1600 |
| BspMAI | CTGCAG | 1 cut(s) | 1951 |
| BspMI | ACCTGC | 2 cut(s) | 333, 1813 |
| BspPI | GGATC | 6 cut(s) | 854, 867, 944, 1049, 1062, 1713 |
| BspT107I | GGYRCC | 1 cut(s) | 850 |
| BspTI | CTTAAG | 1 cut(s) | 1276 |
| BsrDI | GCAATG | 2 cut(s) | 46, 852 |
| BsrI | ACTGG | 5 cut(s) | 224, 430, 886, 1878, 2096 |
| BssMI | GATC | 9 cut(s) | 253, 859, 936, 1054, 1087, 1213, 1389, 1417, 1705 |
| BssNI | GRCGYC | 1 cut(s) | 959 |
| BssSI | CACGAG | 2 cut(s) | 1192, 2088 |
| BssT1I | CCWWGG | 7 cut(s) | 315, 469, 1050, 1369, 1587, 1685, 2213 |
| Bst2BI | CACGAG | 2 cut(s) | 1192, 2088 |
| Bst2UI | CCWGG | 4 cut(s) | 240, 366, 865, 2344 |
| Bst4CI | ACNGT | 9 cut(s) | 281, 309, 736, 901, 1085, 1094, 1099, 1338, 1695 |
| Bst6I | CTCTTC | 3 cut(s) | 201, 1518, 2246 |
| BstACI | GRCGYC | 1 cut(s) | 959 |
| BstAFI | CTTAAG | 1 cut(s) | 1276 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 425 |
| BstC8I | GCNNGC | 5 cut(s) | 183, 2068, 2328, 2537, 2577 |
| BstDSI | CCRYGG | 4 cut(s) | 315, 469, 1587, 1838 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 397 |
| BstF5I | GGATG | 2 cut(s) | 510, 661 |
| BstHHI | GCGC | 1 cut(s) | 2261 |
| BstKTI | GATC | 9 cut(s) | 256, 862, 939, 1057, 1090, 1216, 1392, 1420, 1708 |
| BstMAI | GTCTC | 3 cut(s) | 798, 950, 2055 |
| BstMBI | GATC | 9 cut(s) | 253, 859, 936, 1054, 1087, 1213, 1389, 1417, 1705 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 235, 425, 1631, 1766, 1865, 1949 |
| BstNI | CCWGG | 4 cut(s) | 240, 366, 865, 2344 |
| BstNSI | RCATGY | 1 cut(s) | 2320 |
| BstSCI | CCNGG | 4 cut(s) | 238, 364, 863, 2342 |
| BstSFI | CTRYAG | 2 cut(s) | 1098, 1947 |
| BstV2I | GAAGAC | 1 cut(s) | 2616 |
| BstX2I | RGATCY | 3 cut(s) | 859, 1054, 1705 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 437 |
| BstYI | RGATCY | 3 cut(s) | 859, 1054, 1705 |
| BsuI | GTATCC | 1 cut(s) | 2068 |
| BsuRI | GGCC | 5 cut(s) | 1142, 1625, 1649, 1859, 2298 |
| BtgI | CCRYGG | 4 cut(s) | 315, 469, 1587, 1838 |
| BtgZI | GCGATG | 2 cut(s) | 2367, 2466 |
| BtrI | CACGTC | 1 cut(s) | 90 |
| BtsCI | GGATG | 2 cut(s) | 510, 661 |
| BtsI | GCAGTG | 1 cut(s) | 924 |
| BtsIMutI | CAGTG | 6 cut(s) | 286, 924, 1090, 1542, 1691, 2475 |
| BveI | ACCTGC | 2 cut(s) | 333, 1813 |
| Cac8I | GCNNGC | 5 cut(s) | 183, 2068, 2328, 2537, 2577 |
| CfoI | GCGC | 1 cut(s) | 2261 |
| Cfr13I | GGNCC | 5 cut(s) | 54, 322, 333, 1599, 1931 |
| Csp6I | GTAC | 8 cut(s) | 245, 277, 492, 567, 868, 1095, 1802, 1818 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 28, 63 |
| CviAII | CATG | 7 cut(s) | 18, 316, 470, 593, 1588, 2131, 2317 |
| CviQI | GTAC | 8 cut(s) | 245, 277, 492, 567, 868, 1095, 1802, 1818 |
| DpnI | GATC | 9 cut(s) | 255, 861, 938, 1056, 1089, 1215, 1391, 1419, 1707 |
| DpnII | GATC | 9 cut(s) | 253, 859, 936, 1054, 1087, 1213, 1389, 1417, 1705 |
| DraI | TTTAAA | 1 cut(s) | 1171 |
| EaeI | YGGCCR | 1 cut(s) | 1140 |
| Eam1104I | CTCTTC | 3 cut(s) | 201, 1518, 2246 |
| EarI | CTCTTC | 3 cut(s) | 201, 1518, 2246 |
| EciI | GGCGGA | 1 cut(s) | 335 |
| Ecl136II | GAGCTC | 2 cut(s) | 777, 1432 |
| Eco130I | CCWWGG | 7 cut(s) | 315, 469, 1050, 1369, 1587, 1685, 2213 |
| Eco147I | AGGCCT | 1 cut(s) | 1625 |
| Eco24I | GRGCYC | 2 cut(s) | 779, 1434 |
| Eco32I | GATATC | 1 cut(s) | 1105 |
| Eco47I | GGWCC | 5 cut(s) | 54, 322, 333, 1599, 1931 |
| Eco53kI | GAGCTC | 2 cut(s) | 777, 1432 |
| Eco57I | CTGAAG | 4 cut(s) | 1053, 1181, 2100, 2499 |
| Eco88I | CYCGRG | 3 cut(s) | 5, 799, 1019 |
| EcoICRI | GAGCTC | 2 cut(s) | 777, 1432 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 397 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1599 |
| EcoRI | GAATTC | 2 cut(s) | 1013, 1286 |
| EcoRII | CCWGG | 4 cut(s) | 238, 364, 863, 2342 |
| EcoRV | GATATC | 1 cut(s) | 1105 |
| EcoT14I | CCWWGG | 7 cut(s) | 315, 469, 1050, 1369, 1587, 1685, 2213 |
| EcoT38I | GRGCYC | 2 cut(s) | 779, 1434 |
| ErhI | CCWWGG | 7 cut(s) | 315, 469, 1050, 1369, 1587, 1685, 2213 |
| Esp3I | CGTCTC | 2 cut(s) | 950, 2055 |
| FaeI | CATG | 7 cut(s) | 21, 319, 473, 596, 1591, 2134, 2320 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 368, 400, 389, 421, 1738, 1770, 2010, 2042 |
| FaqI | GGGAC | 2 cut(s) | 778, 1612 |
| FatI | CATG | 7 cut(s) | 17, 315, 469, 592, 1587, 2130, 2316 |
| FbaI | TGATCA | 2 cut(s) | 253, 1087 |
| FokI | GGATG | 2 cut(s) | 517, 668 |
| FriOI | GRGCYC | 2 cut(s) | 779, 1434 |
| FspBI | CTAG | 4 cut(s) | 1478, 2138, 2514, 2580 |
| GlaI | GCGC | 1 cut(s) | 2260 |
| GsaI | CCCAGC | 1 cut(s) | 2240 |
| GsuI | CTGGAG | 3 cut(s) | 261, 1230, 1760 |
| HaeIII | GGCC | 5 cut(s) | 1142, 1625, 1649, 1859, 2298 |
| HhaI | GCGC | 1 cut(s) | 2261 |
| Hin1I | GRCGYC | 1 cut(s) | 959 |
| Hin1II | CATG | 7 cut(s) | 21, 319, 473, 596, 1591, 2134, 2320 |
| Hin6I | GCGC | 1 cut(s) | 2259 |
| HinP1I | GCGC | 1 cut(s) | 2259 |
| HindIII | AAGCTT | 2 cut(s) | 266, 2303 |
| HinfI | GANTC | 9 cut(s) | 379, 481, 908, 997, 1022, 2045, 2530, 2561, 2588 |
| HphI | GGTGA | 4 cut(s) | 485, 851, 2343, 2539 |
| Hpy166II | GTNNAC | 4 cut(s) | 292, 996, 1114, 2464 |
| Hpy188I | TCNGA | 9 cut(s) | 258, 451, 976, 1033, 1534, 1983, 2523, 2566, 2593 |
| Hpy8I | GTNNAC | 4 cut(s) | 292, 996, 1114, 2464 |
| Hpy99I | CGWCG | 1 cut(s) | 2150 |
| HpyCH4III | ACNGT | 9 cut(s) | 281, 309, 736, 901, 1085, 1094, 1099, 1338, 1695 |
| HpyCH4IV | ACGT | 2 cut(s) | 89, 959 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 235, 425, 1631, 1766, 1865, 1949 |
| HpySE526I | ACGT | 2 cut(s) | 89, 959 |
| Hsp92I | GRCGYC | 1 cut(s) | 959 |
| Hsp92II | CATG | 7 cut(s) | 21, 319, 473, 596, 1591, 2134, 2320 |
| HspAI | GCGC | 1 cut(s) | 2259 |
| Ksp22I | TGATCA | 2 cut(s) | 253, 1087 |
| Kzo9I | GATC | 9 cut(s) | 253, 859, 936, 1054, 1087, 1213, 1389, 1417, 1705 |
| LmnI | GCTCC | 8 cut(s) | 395, 525, 774, 1437, 1553, 1909, 1948, 2500 |
| LweI | GCATC | 5 cut(s) | 832, 1702, 2234, 2390, 2590 |
| MaeI | CTAG | 4 cut(s) | 1478, 2138, 2514, 2580 |
| MaeII | ACGT | 2 cut(s) | 89, 959 |
| MaeIII | GTNAC | 5 cut(s) | 281, 572, 960, 1565, 1689 |
| MalI | GATC | 9 cut(s) | 255, 861, 938, 1056, 1089, 1215, 1391, 1419, 1707 |
| MboI | GATC | 9 cut(s) | 253, 859, 936, 1054, 1087, 1213, 1389, 1417, 1705 |
| MfeI | CAATTG | 2 cut(s) | 174, 1593 |
| MflI | RGATCY | 3 cut(s) | 859, 1054, 1705 |
| MhlI | GDGCHC | 4 cut(s) | 604, 779, 1434, 1558 |
| MlyI | GAGTC | 4 cut(s) | 490, 917, 991, 1031 |
| MmeI | TCCRAC | 4 cut(s) | 755, 1311, 1459, 2616 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1254 |
| MseI | TTAA | 7 cut(s) | 273, 396, 744, 1170, 1277, 1340, 2309 |
| MslI | CAYNNNNRTG | 4 cut(s) | 471, 503, 597, 1119 |
| MspA1I | CMGCKG | 1 cut(s) | 2174 |
| MspCI | CTTAAG | 1 cut(s) | 1276 |
| MspR9I | CCNGG | 4 cut(s) | 240, 366, 865, 2344 |
| MunI | CAATTG | 2 cut(s) | 174, 1593 |
| Mva1269I | GAATGC | 1 cut(s) | 454 |
| MvaI | CCWGG | 4 cut(s) | 240, 366, 865, 2344 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 235, 425, 1631, 1766, 1865, 1949 |
| NcoI | CCATGG | 3 cut(s) | 315, 469, 1587 |
| NdeII | GATC | 9 cut(s) | 253, 859, 936, 1054, 1087, 1213, 1389, 1417, 1705 |
| NlaIII | CATG | 7 cut(s) | 21, 319, 473, 596, 1591, 2134, 2320 |
| NlaIV | GGNNCC | 5 cut(s) | 641, 852, 861, 1056, 1600 |
| NmuCI | GTSAC | 4 cut(s) | 281, 960, 1565, 1689 |
| NspI | RCATGY | 1 cut(s) | 2320 |
| OliI | CACNNNNGTG | 2 cut(s) | 471, 1119 |
| PaeR7I | CTCGAG | 1 cut(s) | 1019 |
| PceI | AGGCCT | 1 cut(s) | 1625 |
| PciI | ACATGT | 1 cut(s) | 2316 |
| PctI | GAATGC | 1 cut(s) | 454 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1254 |
| PfeI | GAWTC | 5 cut(s) | 379, 2045, 2530, 2561, 2588 |
| PleI | GAGTC | 4 cut(s) | 489, 916, 991, 1030 |
| PpsI | GAGTC | 4 cut(s) | 489, 916, 991, 1030 |
| PpuMI | RGGWCCY | 1 cut(s) | 1599 |
| PscI | ACATGT | 1 cut(s) | 2316 |
| PsiI | TTATAA | 3 cut(s) | 69, 795, 1008 |
| Psp124BI | GAGCTC | 2 cut(s) | 779, 1434 |
| Psp5II | RGGWCCY | 1 cut(s) | 1599 |
| Psp6I | CCWGG | 4 cut(s) | 238, 364, 863, 2342 |
| PspFI | CCCAGC | 1 cut(s) | 2236 |
| PspGI | CCWGG | 4 cut(s) | 238, 364, 863, 2342 |
| PspN4I | GGNNCC | 5 cut(s) | 641, 852, 861, 1056, 1600 |
| PspPI | GGNCC | 5 cut(s) | 54, 322, 333, 1599, 1931 |
| PspPPI | RGGWCCY | 1 cut(s) | 1599 |
| PspXI | VCTCGAGB | 1 cut(s) | 1019 |
| PstI | CTGCAG | 1 cut(s) | 1951 |
| PsuI | RGATCY | 3 cut(s) | 859, 1054, 1705 |
| PvuII | CAGCTG | 1 cut(s) | 2174 |
| RsaI | GTAC | 8 cut(s) | 246, 278, 493, 568, 869, 1096, 1803, 1819 |
| RsaNI | GTAC | 8 cut(s) | 245, 277, 492, 567, 868, 1095, 1802, 1818 |
| RseI | CAYNNNNRTG | 4 cut(s) | 471, 503, 597, 1119 |
| SacI | GAGCTC | 2 cut(s) | 779, 1434 |
| SaqAI | TTAA | 7 cut(s) | 273, 396, 744, 1170, 1277, 1340, 2309 |
| Sau3AI | GATC | 9 cut(s) | 253, 859, 936, 1054, 1087, 1213, 1389, 1417, 1705 |
| Sau96I | GGNCC | 5 cut(s) | 54, 322, 333, 1599, 1931 |
| ScaI | AGTACT | 2 cut(s) | 493, 1096 |
| SchI | GAGTC | 4 cut(s) | 490, 917, 991, 1031 |
| ScrFI | CCNGG | 4 cut(s) | 240, 366, 865, 2344 |
| SduI | GDGCHC | 4 cut(s) | 604, 779, 1434, 1558 |
| SfaNI | GCATC | 5 cut(s) | 832, 1702, 2234, 2390, 2590 |
| SfcI | CTRYAG | 2 cut(s) | 1098, 1947 |
| Sfr274I | CTCGAG | 1 cut(s) | 1019 |
| SinI | GGWCC | 5 cut(s) | 54, 322, 333, 1599, 1931 |
| SlaI | CTCGAG | 1 cut(s) | 1019 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 471, 503, 597, 1119 |
| SmlI | CTYRAG | 7 cut(s) | 999, 1019, 1259, 1276, 1325, 1427, 2009 |
| SmoI | CTYRAG | 7 cut(s) | 999, 1019, 1259, 1276, 1325, 1427, 2009 |
| SseBI | AGGCCT | 1 cut(s) | 1625 |
| SsiI | CCGC | 5 cut(s) | 320, 1139, 1655, 2219, 2403 |
| SspI | AATATT | 1 cut(s) | 65 |
| SspMI | CTAG | 4 cut(s) | 1478, 2138, 2514, 2580 |
| SstI | GAGCTC | 2 cut(s) | 779, 1434 |
| StuI | AGGCCT | 1 cut(s) | 1625 |
| StyD4I | CCNGG | 4 cut(s) | 238, 364, 863, 2342 |
| StyI | CCWWGG | 7 cut(s) | 315, 469, 1050, 1369, 1587, 1685, 2213 |
| TaaI | ACNGT | 9 cut(s) | 281, 309, 736, 901, 1085, 1094, 1099, 1338, 1695 |
| TaiI | ACGT | 2 cut(s) | 92, 962 |
| TaqI | TCGA | 4 cut(s) | 619, 1020, 1216, 1731 |
| TatI | WGTACW | 4 cut(s) | 244, 276, 491, 1094 |
| TauI | GCSGC | 2 cut(s) | 1142, 2222 |
| TfiI | GAWTC | 5 cut(s) | 379, 2045, 2530, 2561, 2588 |
| Tru1I | TTAA | 7 cut(s) | 273, 396, 744, 1170, 1277, 1340, 2309 |
| Tru9I | TTAA | 7 cut(s) | 273, 396, 744, 1170, 1277, 1340, 2309 |
| TscAI | CASTG | 6 cut(s) | 286, 931, 1090, 1549, 1698, 2482 |
| TseFI | GTSAC | 4 cut(s) | 281, 960, 1565, 1689 |
| Tsp45I | GTSAC | 4 cut(s) | 281, 960, 1565, 1689 |
| TspDTI | ATGAA | 6 cut(s) | 113, 699, 1051, 1680, 2016, 2280 |
| TspGWI | ACGGA | 4 cut(s) | 330, 977, 1573, 1855 |
| TspRI | CASTG | 6 cut(s) | 286, 931, 1090, 1549, 1698, 2482 |
| Vha464I | CTTAAG | 1 cut(s) | 1276 |
| VpaK11BI | GGWCC | 5 cut(s) | 54, 322, 333, 1599, 1931 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 397 |
| XapI | RAATTY | 6 cut(s) | 1013, 1166, 1235, 1254, 1286, 2059 |
| XceI | RCATGY | 1 cut(s) | 2320 |
| XcmI | CCANNNNNNNNNTGG | 5 cut(s) | 343, 861, 990, 1692, 1885 |
| XhoI | CTCGAG | 1 cut(s) | 1019 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1254 |
| XspI | CTAG | 4 cut(s) | 1478, 2138, 2514, 2580 |
| ZraI | GACGTC | 1 cut(s) | 960 |
| ZrmI | AGTACT | 2 cut(s) | 493, 1096 |