Rorug01G0423400

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001532
Physical Location & Seq
Forward (+)
52411480 .. 52414425
2946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0423400.1

Sequence Viewer

Length: 1572 bp
ATGGGACCAATGGAAGAGGGAGCTGCTGGGACAAATTACACCAAGCCCTTGGGGATTCGATACATAGAGTACATAAAAAGATCGAAGATTTCATACAAAACCCACCAAGTTATTGTTTTGATTGTTACTTTTCTAGCCTATGCAAGCTACCATGCCGCCCGGAAAACCACTAGTGTGGTCAAGAGTACTCTTGATCCTGCATCCGATGAGGTAGGGTTGAATTTCTTGCCATGGAGAATGAGTTACTTGAGTCAACCAGGGGAAAGAAGAAGACGGTTTTCGTGGATTCTTGGAGATGGATGGGCACCATTTGATGGATCAGATGGAACAGCCTTGCTTGGTGAAATTGATGTAGCTTTTCTAGCTGTTTATGCTATAGGAATGTACTTTTCTGGTCATTTGGGTGATAGAATAAACTTGAGGATTTTTTTGACTATAGGAATGGTGGGAACAGGTGTGTTTACTTCGCTGTTTGGAATTGGGTATTGGGGAAACATTCACAGCTTCTATTACTACCTTGGGGTTCAAATGCTTGCTGGTTTGTTCCAATCGACGGGGTGGCCTTCGGTGGTTGCAGTGGTTGGTAATTGGTTTGGGAAGAAGAAGAGAGGGTTGATAATGGGGATATGGAATGCTCACACTTCTGTTGGGAACATTACTGGGTCTTTGGTTGCTTCTGCTCTATTGCAATATGGATGGGGTTGGTCTTTTGTAGTGCCTGGTTTGATGATTGCTACAATTGGTGTGGTGGTTTTTCTGTTCTTGCCGGTTAGTCCTGAGTCTGTGGGAGTTAGCGAAGAGGATGAATTAGACTCTCCAAAGAAAAGCGGGGCGGAAGAGACTGAACCATTGTTGGCATCAAGGGCAGAGGTGAAGGATCAACCTGTGGGGTTCATTGAAGCTTGGAAAATTCCTGGTGTTGCTCCTTTTGCTCTTTGCCTGTTCTTTTCCAAATTGGTGGCTTATACATTTCTCTATTGGCTTCCTTTCTACATTAGCCATACAGCTATTGATGGGCAGTACTTATCAGATGCGGCGTCTGGTACCTTATCGACATTGTTTGATGTAGGAGGTGTGCTTGGAGGAATCCTTGCCGGACATATTTCTGACCGGTTAGATGCTAGAGCGATAACAGCTGCTAGTTTCATGTATTGTGCTATCCCTGCTCTCTTTTTATACCGGAGCTATGGAAGCATATCCTTGACTGTAAACATTATCCTTATGTTCATCACCGGCATGTTTGTTAACGGGCCATATGCTCTAATAACAACGGCCGTCTCAGCAGACCTGGGAACACACAGCTCGCTGCGTGGAAACTCGAGGGCATTGGCAACTGTAACTGCTATTATCGATGGAACAGGCTCCGTAGGGGCTGCAATCGGTCCATTGGTAACTGGCTACATTTCTGCTGAGAGCTGGAGTGCAGTTTTCACAATGTTGATGGGAGCAGCTCTAATTGCAGGGTTGCTTCTAACTAGGCTTGTTATAGCCGAGGTGGCTGCAAAGATCGAAGAATCAAGAGCGCGCAGGGGGTCTACATCAAGGTCTGAGCATGCAGTAGTTGATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 map01524
Pfam Domains
Protein Families
DNA_gyraseB DNA_topoisoIV HATPase_c TOPRIM_C Toprim

Protein Analysis

523

Amino Acids

56.26

Weight (kDa)

6.76

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 113 - 432 2.7e-42 Major Facilitator Superfamily
MFS_1 PF07690 345 - 506 2.2e-11 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1043
AccB1I GGYRCC 2 cut(s) 304, 1043
AccB7I CCANNNNNTGG 1 cut(s) 314
AccI GTMKAC 1 cut(s) 1535
AccII CGCG 1 cut(s) 1525
AciI CCGC 4 cut(s) 156, 828, 833, 1034
AclWI GGATC 3 cut(s) 188, 325, 885
AcoI YGGCCR 1 cut(s) 1272
AcsI RAATTY 2 cut(s) 220, 909
AcyI GRCGYC 1 cut(s) 1037
AfaI GTAC 5 cut(s) 71, 187, 386, 1022, 1045
AfiI CCNNNNNNNGG 2 cut(s) 314, 553
AgeI ACCGGT 1 cut(s) 1110
AgsI TTSAA 3 cut(s) 220, 527, 899
AhlI ACTAGT 1 cut(s) 170
AjnI CCWGG 4 cut(s) 256, 718, 913, 1287
AjuI GAANNNNNNNTTGG 2 cut(s) 469, 501
AleI CACNNNNGTG 1 cut(s) 173
Alw26I GTCTC 2 cut(s) 833, 1282
AlwI GGATC 3 cut(s) 188, 325, 885
Ama87I CYCGRG 1 cut(s) 1318
AoxI GGCC 3 cut(s) 560, 1250, 1272
ApeKI GCWGC 6 cut(s) 23, 1136, 1306, 1373, 1448, 1499
ApoI RAATTY 2 cut(s) 220, 909
AsiGI ACCGGT 1 cut(s) 1110
Asp718I GGTACC 1 cut(s) 1043
AspLEI GCGC 2 cut(s) 1525, 1527
AspS9I GGNCC 3 cut(s) 5, 1250, 1382
AsuC2I CCSGG 1 cut(s) 160
AsuHPI GGTGA 4 cut(s) 353, 416, 883, 1222
AvaI CYCGRG 1 cut(s) 1318
AvaII GGWCC 2 cut(s) 5, 1382
BaeGI GKGCMC 1 cut(s) 307
BanI GGYRCC 2 cut(s) 304, 1043
BarI GAAGNNNNNNTAC 2 cut(s) 77, 109
BbsI GAAGAC 1 cut(s) 277
BbvI GCAGC 6 cut(s) 10, 1123, 1293, 1360, 1460, 1486
BccI CCATC 8 cut(s) 290, 294, 308, 317, 690, 1007, 1346, 1435
BceAI ACGGC 2 cut(s) 1259, 1287
BcgI CGANNNNNNTGC 2 cut(s) 1481, 1515
BciT130I CCWGG 4 cut(s) 258, 720, 915, 1289
BcnI CCSGG 1 cut(s) 160
BcoDI GTCTC 2 cut(s) 833, 1282
BcuI ACTAGT 1 cut(s) 170
BfaI CTAG 6 cut(s) 134, 171, 362, 1122, 1140, 1476
BfmI CTRYAG 2 cut(s) 375, 435
BglI GCCNNNNNGGC 1 cut(s) 1496
BisI GCNGC 8 cut(s) 24, 156, 1035, 1137, 1307, 1374, 1449, 1500
BlsI GCNGC 8 cut(s) 25, 157, 1036, 1138, 1308, 1375, 1450, 1501
BmcAI AGTACT 2 cut(s) 187, 1022
Bme1390I CCNGG 5 cut(s) 160, 258, 720, 915, 1289
Bme18I GGWCC 2 cut(s) 5, 1382
BmeT110I CYCGRG 1 cut(s) 1318
BmgT120I GGNCC 3 cut(s) 5, 1250, 1382
BmiI GGNNCC 4 cut(s) 6, 306, 1045, 1363
BmrFI CCNGG 5 cut(s) 160, 258, 720, 915, 1289
BmrI ACTGGG 1 cut(s) 669
BmsI GCATC 4 cut(s) 209, 866, 1021, 1108
BmuI ACTGGG 1 cut(s) 669
BpiI GAAGAC 1 cut(s) 277
BpmI CTGGAG 1 cut(s) 1438
BpuEI CTTGAG 2 cut(s) 268, 439
BpuMI CCSGG 1 cut(s) 160
Bsa29I ATCGAT 1 cut(s) 1350
BsaHI GRCGYC 1 cut(s) 1037
BsaJI CCNNGG 6 cut(s) 48, 230, 257, 517, 1288, 1491
BsaWI WCCGGW 2 cut(s) 1110, 1179
BsaXI ACNNNNNCTCC 2 cut(s) 226, 256
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 553
Bse118I RCCGGY 3 cut(s) 766, 1110, 1232
Bse1I ACTGG 2 cut(s) 664, 1399
BseBI CCWGG 4 cut(s) 258, 720, 915, 1289
BseCI ATCGAT 1 cut(s) 1350
BseDI CCNNGG 6 cut(s) 48, 230, 257, 517, 1288, 1491
BseGI GGATG 4 cut(s) 200, 305, 701, 808
BseLI CCNNNNNNNGG 2 cut(s) 314, 553
BseMII CTCAG 4 cut(s) 768, 1293, 1401, 1539
BseNI ACTGG 2 cut(s) 664, 1399
BsePI GCGCGC 1 cut(s) 1523
BseSI GKGCMC 1 cut(s) 307
BseX3I CGGCCG 1 cut(s) 1272
BseXI GCAGC 6 cut(s) 10, 1123, 1293, 1360, 1460, 1486
BseYI CCCAGC 1 cut(s) 26
BsgI GTGCAG 1 cut(s) 1443
Bsh1236I CGCG 1 cut(s) 1525
Bsh1285I CGRYCG 1 cut(s) 1275
BshFI GGCC 3 cut(s) 562, 1252, 1274
BshNI GGYRCC 2 cut(s) 304, 1043
BshTI ACCGGT 1 cut(s) 1110
BshVI ATCGAT 1 cut(s) 1350
BsiEI CGRYCG 1 cut(s) 1275
BsiHKCI CYCGRG 1 cut(s) 1318
BsiSI CCGG 6 cut(s) 160, 767, 1095, 1111, 1180, 1233
BslFI GGGAC 2 cut(s) 18, 43
BslI CCNNNNNNNGG 2 cut(s) 314, 553
BsmAI GTCTC 2 cut(s) 833, 1282
BsmBI CGTCTC 1 cut(s) 1282
BsmFI GGGAC 2 cut(s) 18, 43
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 3 cut(s) 562, 1252, 1274
BsoBI CYCGRG 1 cut(s) 1318
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 5 cut(s) 80, 193, 317, 877, 1506
Bsp19I CCATGG 1 cut(s) 230
BspACI CCGC 4 cut(s) 156, 828, 833, 1034
BspANI GGCC 3 cut(s) 562, 1252, 1274
BspCNI CTCAG 4 cut(s) 769, 1292, 1402, 1540
BspDI ATCGAT 1 cut(s) 1350
BspFNI CGCG 1 cut(s) 1525
BspLI GGNNCC 4 cut(s) 6, 306, 1045, 1363
BspPI GGATC 3 cut(s) 188, 325, 885
BspT107I GGYRCC 2 cut(s) 304, 1043
BsrFI RCCGGY 3 cut(s) 766, 1110, 1232
BsrI ACTGG 2 cut(s) 664, 1399
BssAI RCCGGY 3 cut(s) 766, 1110, 1232
BssECI CCNNGG 6 cut(s) 48, 230, 257, 517, 1288, 1491
BssHII GCGCGC 1 cut(s) 1523
BssMI GATC 5 cut(s) 80, 193, 317, 877, 1506
BssNI GRCGYC 1 cut(s) 1037
BssT1I CCWWGG 3 cut(s) 48, 230, 517
Bst2UI CCWGG 4 cut(s) 258, 720, 915, 1289
Bst4CI ACNGT 3 cut(s) 276, 1207, 1336
Bst6I CTCTTC 4 cut(s) 9, 599, 792, 831
BstACI GRCGYC 1 cut(s) 1037
BstC8I GCNNGC 5 cut(s) 145, 534, 1304, 1525, 1554
BstDEI CTNAG 4 cut(s) 777, 1279, 1410, 1548
BstDSI CCRYGG 1 cut(s) 230
BstF5I GGATG 4 cut(s) 200, 305, 701, 808
BstFNI CGCG 1 cut(s) 1525
BstHHI GCGC 2 cut(s) 1525, 1527
BstKTI GATC 5 cut(s) 83, 196, 320, 880, 1509
BstMAI GTCTC 2 cut(s) 833, 1282
BstMBI GATC 5 cut(s) 80, 193, 317, 877, 1506
BstMCI CGRYCG 1 cut(s) 1275
BstNI CCWGG 4 cut(s) 258, 720, 915, 1289
BstNSI RCATGY 2 cut(s) 1240, 1556
BstSCI CCNGG 5 cut(s) 158, 256, 718, 913, 1287
BstSFI CTRYAG 2 cut(s) 375, 435
BstSLI GKGCMC 1 cut(s) 307
BstUI CGCG 1 cut(s) 1525
BstV1I GCAGC 6 cut(s) 10, 1123, 1293, 1360, 1460, 1486
BstV2I GAAGAC 1 cut(s) 277
BstXI CCANNNNNNTGG 3 cut(s) 49, 175, 958
BstZI CGGCCG 1 cut(s) 1272
Bsu15I ATCGAT 1 cut(s) 1350
BsuRI GGCC 3 cut(s) 562, 1252, 1274
BsuTUI ATCGAT 1 cut(s) 1350
BtgI CCRYGG 1 cut(s) 230
BtsCI GGATG 4 cut(s) 200, 305, 701, 808
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 1 cut(s) 582
Cac8I GCNNGC 5 cut(s) 145, 534, 1304, 1525, 1554
CfoI GCGC 2 cut(s) 1525, 1527
Cfr10I RCCGGY 3 cut(s) 766, 1110, 1232
Cfr13I GGNCC 3 cut(s) 5, 1250, 1382
ClaI ATCGAT 1 cut(s) 1350
CseI GACGC 1 cut(s) 1026
Csp6I GTAC 5 cut(s) 70, 186, 385, 1021, 1044
CspAI ACCGGT 1 cut(s) 1110
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviAII CATG 5 cut(s) 152, 231, 1147, 1237, 1553
CviQI GTAC 5 cut(s) 70, 186, 385, 1021, 1044
DdeI CTNAG 4 cut(s) 777, 1279, 1410, 1548
DpnI GATC 5 cut(s) 82, 195, 319, 879, 1508
DpnII GATC 5 cut(s) 80, 193, 317, 877, 1506
EaeI YGGCCR 1 cut(s) 1272
EagI CGGCCG 1 cut(s) 1272
Eam1104I CTCTTC 4 cut(s) 9, 599, 792, 831
EarI CTCTTC 4 cut(s) 9, 599, 792, 831
EciI GGCGGA 1 cut(s) 848
EclXI CGGCCG 1 cut(s) 1272
Eco130I CCWWGG 3 cut(s) 48, 230, 517
Eco47I GGWCC 2 cut(s) 5, 1382
Eco52I CGGCCG 1 cut(s) 1272
Eco88I CYCGRG 1 cut(s) 1318
EcoRII CCWGG 4 cut(s) 256, 718, 913, 1287
EcoT14I CCWWGG 3 cut(s) 48, 230, 517
ErhI CCWWGG 3 cut(s) 48, 230, 517
Esp3I CGTCTC 1 cut(s) 1282
FaeI CATG 5 cut(s) 155, 234, 1150, 1240, 1556
FaqI GGGAC 2 cut(s) 18, 43
FatI CATG 5 cut(s) 151, 230, 1146, 1236, 1552
FauI CCCGC 1 cut(s) 821
FauNDI CATATG 1 cut(s) 1255
FblI GTMKAC 1 cut(s) 1535
Fnu4HI GCNGC 8 cut(s) 24, 156, 1035, 1137, 1307, 1374, 1449, 1500
FokI GGATG 4 cut(s) 187, 312, 708, 815
Fsp4HI GCNGC 8 cut(s) 24, 156, 1035, 1137, 1307, 1374, 1449, 1500
FspBI CTAG 6 cut(s) 134, 171, 362, 1122, 1140, 1476
GlaI GCGC 2 cut(s) 1524, 1526
GluI GCNGC 8 cut(s) 24, 156, 1035, 1137, 1307, 1374, 1449, 1500
GsaI CCCAGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 1438
HaeIII GGCC 3 cut(s) 562, 1252, 1274
HapII CCGG 6 cut(s) 160, 767, 1095, 1111, 1180, 1233
HgaI GACGC 1 cut(s) 1026
HhaI GCGC 2 cut(s) 1525, 1527
Hin1I GRCGYC 1 cut(s) 1037
Hin1II CATG 5 cut(s) 155, 234, 1150, 1240, 1556
Hin6I GCGC 2 cut(s) 1523, 1525
HinP1I GCGC 2 cut(s) 1523, 1525
HincII GTYRAC 2 cut(s) 254, 1246
HindII GTYRAC 2 cut(s) 254, 1246
HindIII AAGCTT 1 cut(s) 900
HinfI GANTC 7 cut(s) 55, 250, 286, 779, 812, 1086, 1514
HpaI GTTAAC 1 cut(s) 1246
HpaII CCGG 6 cut(s) 160, 767, 1095, 1111, 1180, 1233
HphI GGTGA 4 cut(s) 353, 416, 883, 1222
Hpy166II GTNNAC 5 cut(s) 254, 462, 1210, 1246, 1536
Hpy188I TCNGA 5 cut(s) 205, 322, 1030, 1108, 1549
Hpy188III TCNNGA 4 cut(s) 181, 191, 776, 1518
Hpy8I GTNNAC 5 cut(s) 254, 462, 1210, 1246, 1536
Hpy99I CGWCG 1 cut(s) 556
HpyAV CCTTC 2 cut(s) 573, 868
HpyCH4III ACNGT 3 cut(s) 276, 1207, 1336
HpyCH4V TGCA 9 cut(s) 143, 200, 575, 688, 1376, 1424, 1460, 1502, 1556
HpyF3I CTNAG 4 cut(s) 777, 1279, 1410, 1548
Hsp92I GRCGYC 1 cut(s) 1037
Hsp92II CATG 5 cut(s) 155, 234, 1150, 1240, 1556
HspAI GCGC 2 cut(s) 1523, 1525
KpnI GGTACC 1 cut(s) 1047
KspAI GTTAAC 1 cut(s) 1246
Kzo9I GATC 5 cut(s) 80, 193, 317, 877, 1506
LmnI GCTCC 5 cut(s) 20, 928, 1182, 1367, 1445
Lsp1109I GCAGC 6 cut(s) 10, 1123, 1293, 1360, 1460, 1486
LweI GCATC 4 cut(s) 209, 866, 1021, 1108
MaeI CTAG 6 cut(s) 134, 171, 362, 1122, 1140, 1476
MaeIII GTNAC 4 cut(s) 124, 242, 1336, 1390
MalI GATC 5 cut(s) 82, 195, 319, 879, 1508
MboI GATC 5 cut(s) 80, 193, 317, 877, 1506
MfeI CAATTG 1 cut(s) 738
MhlI GDGCHC 1 cut(s) 307
MlyI GAGTC 3 cut(s) 259, 788, 806
MseI TTAA 1 cut(s) 1245
MslI CAYNNNNRTG 3 cut(s) 173, 402, 1235
MspA1I CMGCKG 1 cut(s) 1136
MspI CCGG 6 cut(s) 160, 767, 1095, 1111, 1180, 1233
MspR9I CCNGG 5 cut(s) 160, 258, 720, 915, 1289
MunI CAATTG 1 cut(s) 738
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 4 cut(s) 258, 720, 915, 1289
MvnI CGCG 1 cut(s) 1525
NciI CCSGG 1 cut(s) 160
NcoI CCATGG 1 cut(s) 230
NdeI CATATG 1 cut(s) 1255
NdeII GATC 5 cut(s) 80, 193, 317, 877, 1506
NlaIII CATG 5 cut(s) 155, 234, 1150, 1240, 1556
NlaIV GGNNCC 4 cut(s) 6, 306, 1045, 1363
NmeAIII GCCGAG 1 cut(s) 1516
NspI RCATGY 2 cut(s) 1240, 1556
OliI CACNNNNGTG 1 cut(s) 173
PaeI GCATGC 1 cut(s) 1556
PaeR7I CTCGAG 1 cut(s) 1318
PauI GCGCGC 1 cut(s) 1523
PctI GAATGC 1 cut(s) 637
PfeI GAWTC 4 cut(s) 55, 286, 1086, 1514
PflMI CCANNNNNTGG 1 cut(s) 314
PinAI ACCGGT 1 cut(s) 1110
PkrI GCNGC 8 cut(s) 25, 157, 1036, 1138, 1308, 1375, 1450, 1501
PleI GAGTC 3 cut(s) 258, 787, 806
PpsI GAGTC 3 cut(s) 258, 787, 806
Psp6I CCWGG 4 cut(s) 256, 718, 913, 1287
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 4 cut(s) 256, 718, 913, 1287
PspN4I GGNNCC 4 cut(s) 6, 306, 1045, 1363
PspPI GGNCC 3 cut(s) 5, 1250, 1382
PspXI VCTCGAGB 1 cut(s) 1318
PteI GCGCGC 1 cut(s) 1523
PvuII CAGCTG 1 cut(s) 1136
RsaI GTAC 5 cut(s) 71, 187, 386, 1022, 1045
RsaNI GTAC 5 cut(s) 70, 186, 385, 1021, 1044
RseI CAYNNNNRTG 3 cut(s) 173, 402, 1235
SaqAI TTAA 1 cut(s) 1245
SatI GCNGC 8 cut(s) 24, 156, 1035, 1137, 1307, 1374, 1449, 1500
Sau3AI GATC 5 cut(s) 80, 193, 317, 877, 1506
Sau96I GGNCC 3 cut(s) 5, 1250, 1382
ScaI AGTACT 2 cut(s) 187, 1022
SchI GAGTC 3 cut(s) 259, 788, 806
ScrFI CCNGG 5 cut(s) 160, 258, 720, 915, 1289
SduI GDGCHC 1 cut(s) 307
SfaNI GCATC 4 cut(s) 209, 866, 1021, 1108
SfcI CTRYAG 2 cut(s) 375, 435
Sfr274I CTCGAG 1 cut(s) 1318
SinI GGWCC 2 cut(s) 5, 1382
SlaI CTCGAG 1 cut(s) 1318
SmiMI CAYNNNNRTG 3 cut(s) 173, 402, 1235
SmlI CTYRAG 3 cut(s) 247, 418, 1318
SmoI CTYRAG 3 cut(s) 247, 418, 1318
SpeI ACTAGT 1 cut(s) 170
SphI GCATGC 1 cut(s) 1556
SsiI CCGC 4 cut(s) 156, 828, 833, 1034
SspMI CTAG 6 cut(s) 134, 171, 362, 1122, 1140, 1476
StyD4I CCNGG 5 cut(s) 158, 256, 718, 913, 1287
StyI CCWWGG 3 cut(s) 48, 230, 517
TaaI ACNGT 3 cut(s) 276, 1207, 1336
TaqI TCGA 7 cut(s) 58, 83, 551, 1052, 1319, 1350, 1509
TaqII GACCGA 1 cut(s) 1370
TatI WGTACW 4 cut(s) 69, 185, 384, 1020
TauI GCSGC 2 cut(s) 158, 1037
TfiI GAWTC 4 cut(s) 55, 286, 1086, 1514
Tru1I TTAA 1 cut(s) 1245
Tru9I TTAA 1 cut(s) 1245
TscAI CASTG 1 cut(s) 582
TseI GCWGC 6 cut(s) 23, 1136, 1306, 1373, 1448, 1499
TspDTI ATGAA 5 cut(s) 81, 819, 883, 1135, 1216
TspGWI ACGGA 1 cut(s) 1354
TspRI CASTG 1 cut(s) 582
Van91I CCANNNNNTGG 1 cut(s) 314
VpaK11BI GGWCC 2 cut(s) 5, 1382
XapI RAATTY 2 cut(s) 220, 909
XceI RCATGY 2 cut(s) 1240, 1556
XhoI CTCGAG 1 cut(s) 1318
XmiI GTMKAC 1 cut(s) 1535
XspI CTAG 6 cut(s) 134, 171, 362, 1122, 1140, 1476
ZrmI AGTACT 2 cut(s) 187, 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.