Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 291, 2112, 2325 |
| AarI | CACCTGC | 1 cut(s) | 457 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 143 |
| Acc36I | ACCTGC | 2 cut(s) | 48, 457 |
| AccB1I | GGYRCC | 2 cut(s) | 299, 2167 |
| AccI | GTMKAC | 1 cut(s) | 2430 |
| AccIII | TCCGGA | 1 cut(s) | 346 |
| AciI | CCGC | 4 cut(s) | 210, 487, 1744, 2143 |
| AclI | AACGTT | 2 cut(s) | 1926, 2918 |
| AclWI | GGATC | 8 cut(s) | 100, 237, 2171, 2184, 2366, 2379, 2525, 3030 |
| AcoI | YGGCCR | 1 cut(s) | 361 |
| AcsI | RAATTY | 9 cut(s) | 419, 569, 737, 2330, 2421, 2483, 2571, 2582, 2603 |
| AcuI | CTGAAG | 2 cut(s) | 2498, 2841 |
| AcyI | GRCGYC | 1 cut(s) | 300 |
| AdeI | CACNNNGTG | 1 cut(s) | 961 |
| AfaI | GTAC | 8 cut(s) | 38, 461, 1228, 1606, 1885, 2233, 2413, 2874 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 46, 1322, 1867, 2647, 2955, 2956, 3012 |
| AjnI | CCWGG | 3 cut(s) | 51, 1756, 2027 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2360, 2392 |
| AleI | CACNNNNGTG | 1 cut(s) | 1863 |
| Alw21I | GWGCWC | 2 cut(s) | 63, 2751 |
| Alw26I | GTCTC | 4 cut(s) | 107, 163, 2115, 2267 |
| AlwI | GGATC | 8 cut(s) | 100, 237, 2171, 2184, 2366, 2379, 2525, 3030 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2980 |
| Ama87I | CYCGRG | 2 cut(s) | 540, 1523 |
| Aor13HI | TCCGGA | 1 cut(s) | 346 |
| AoxI | GGCC | 5 cut(s) | 49, 172, 326, 361, 2364 |
| ApeKI | GCWGC | 3 cut(s) | 1411, 1741, 2456 |
| ApoI | RAATTY | 9 cut(s) | 419, 569, 737, 2330, 2421, 2483, 2571, 2582, 2603 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 1125, 2571, 2586, 2762 |
| AspLEI | GCGC | 2 cut(s) | 302, 392 |
| AspS9I | GGNCC | 7 cut(s) | 49, 145, 326, 537, 682, 1297, 1572 |
| AsuC2I | CCSGG | 5 cut(s) | 171, 304, 376, 541, 542 |
| AsuHPI | GGTGA | 9 cut(s) | 214, 247, 364, 391, 556, 1568, 1877, 2168, 2582 |
| AvaI | CYCGRG | 2 cut(s) | 540, 1523 |
| AvaII | GGWCC | 5 cut(s) | 145, 537, 682, 1297, 1572 |
| AxyI | CCTNAGG | 2 cut(s) | 2439, 2802 |
| BalI | TGGCCA | 1 cut(s) | 363 |
| BamHI | GGATCC | 2 cut(s) | 2176, 2371 |
| BanI | GGYRCC | 2 cut(s) | 299, 2167 |
| BanII | GRGCYC | 2 cut(s) | 2751, 2996 |
| BbsI | GAAGAC | 4 cut(s) | 23, 118, 1118, 1157 |
| Bbv12I | GWGCWC | 2 cut(s) | 63, 2751 |
| BbvI | GCAGC | 3 cut(s) | 1423, 1728, 2468 |
| BceAI | ACGGC | 5 cut(s) | 313, 397, 403, 457, 2079 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 169, 203 |
| BciT130I | CCWGG | 3 cut(s) | 53, 1758, 2029 |
| BclI | TGATCA | 2 cut(s) | 1464, 1474 |
| BcnI | CCSGG | 5 cut(s) | 171, 304, 376, 541, 542 |
| BcoDI | GTCTC | 4 cut(s) | 107, 163, 2115, 2267 |
| BfaI | CTAG | 3 cut(s) | 1199, 1640, 2426 |
| BfmI | CTRYAG | 3 cut(s) | 41, 1409, 2415 |
| BfoI | RGCGCY | 1 cut(s) | 303 |
| BfuAI | ACCTGC | 2 cut(s) | 48, 457 |
| BglII | AGATCT | 1 cut(s) | 183 |
| BisI | GCNGC | 5 cut(s) | 210, 1412, 1742, 1745, 2457 |
| BlsI | GCNGC | 5 cut(s) | 211, 1413, 1743, 1746, 2458 |
| BmcAI | AGTACT | 3 cut(s) | 1606, 1885, 2413 |
| Bme1390I | CCNGG | 8 cut(s) | 53, 171, 304, 376, 541, 542, 1758, 2029 |
| Bme18I | GGWCC | 5 cut(s) | 145, 537, 682, 1297, 1572 |
| BmeT110I | CYCGRG | 2 cut(s) | 540, 1523 |
| BmgT120I | GGNCC | 7 cut(s) | 49, 145, 326, 537, 682, 1297, 1572 |
| BmrFI | CCNGG | 8 cut(s) | 53, 171, 304, 376, 541, 542, 1758, 2029 |
| BmrI | ACTGGG | 1 cut(s) | 1816 |
| BmsI | GCATC | 5 cut(s) | 629, 1180, 1182, 1465, 1549 |
| BmuI | ACTGGG | 1 cut(s) | 1816 |
| BpiI | GAAGAC | 4 cut(s) | 23, 118, 1118, 1157 |
| BpmI | CTGGAG | 4 cut(s) | 1205, 2547, 2832, 3086 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1257 |
| BpuEI | CTTGAG | 5 cut(s) | 932, 1570, 2597, 2765, 2972 |
| BpuMI | CCSGG | 5 cut(s) | 171, 304, 376, 541, 542 |
| Bsa29I | ATCGAT | 1 cut(s) | 95 |
| BsaHI | GRCGYC | 1 cut(s) | 300 |
| BsaJI | CCNNGG | 9 cut(s) | 169, 414, 540, 643, 758, 1861, 2180, 2367, 2641 |
| BsaWI | WCCGGW | 1 cut(s) | 346 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 104, 134, 2694, 2724 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 46, 1322, 1867, 2647, 2955, 2956, 3012 |
| Bse118I | RCCGGY | 1 cut(s) | 328 |
| Bse1I | ACTGG | 8 cut(s) | 503, 539, 1822, 1881, 2039, 2089, 2203, 2917 |
| Bse21I | CCTNAGG | 2 cut(s) | 2439, 2802 |
| Bse3DI | GCAATG | 3 cut(s) | 846, 1275, 2127 |
| BseAI | TCCGGA | 1 cut(s) | 346 |
| BseBI | CCWGG | 3 cut(s) | 53, 1758, 2029 |
| BseCI | ATCGAT | 1 cut(s) | 95 |
| BseDI | CCNNGG | 9 cut(s) | 169, 414, 540, 643, 758, 1861, 2180, 2367, 2641 |
| BseGI | GGATG | 4 cut(s) | 644, 768, 1195, 1978 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 46, 1322, 1867, 2647, 2955, 2956, 3012 |
| BseMI | GCAATG | 3 cut(s) | 846, 1275, 2127 |
| BseMII | CTCAG | 7 cut(s) | 1230, 1378, 1499, 1833, 2296, 2430, 2793 |
| BseNI | ACTGG | 8 cut(s) | 503, 539, 1822, 1881, 2039, 2089, 2203, 2917 |
| BseRI | GAGGAG | 2 cut(s) | 979, 2456 |
| BseXI | GCAGC | 3 cut(s) | 1423, 1728, 2468 |
| Bsh1285I | CGRYCG | 1 cut(s) | 24 |
| BshFI | GGCC | 5 cut(s) | 51, 174, 328, 363, 2366 |
| BshNI | GGYRCC | 2 cut(s) | 299, 2167 |
| BshVI | ATCGAT | 1 cut(s) | 95 |
| BsiEI | CGRYCG | 1 cut(s) | 24 |
| BsiHKAI | GWGCWC | 2 cut(s) | 63, 2751 |
| BsiHKCI | CYCGRG | 2 cut(s) | 540, 1523 |
| BsiSI | CCGG | 6 cut(s) | 171, 303, 329, 347, 375, 541 |
| BslFI | GGGAC | 9 cut(s) | 152, 668, 1226, 1310, 1382, 1637, 1972, 2087, 2266 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 46, 1322, 1867, 2647, 2955, 2956, 3012 |
| BsmAI | GTCTC | 4 cut(s) | 107, 163, 2115, 2267 |
| BsmBI | CGTCTC | 2 cut(s) | 107, 163 |
| BsmFI | GGGAC | 9 cut(s) | 152, 668, 1226, 1310, 1382, 1637, 1972, 2087, 2266 |
| BsmI | GAATGC | 1 cut(s) | 1846 |
| BsnI | GGCC | 5 cut(s) | 51, 174, 328, 363, 2366 |
| BsoBI | CYCGRG | 2 cut(s) | 540, 1523 |
| Bsp1286I | GDGCHC | 3 cut(s) | 63, 2751, 2996 |
| Bsp13I | TCCGGA | 1 cut(s) | 346 |
| Bsp19I | CCATGG | 1 cut(s) | 1861 |
| BspACI | CCGC | 4 cut(s) | 210, 487, 1744, 2143 |
| BspANI | GGCC | 5 cut(s) | 51, 174, 328, 363, 2366 |
| BspCNI | CTCAG | 7 cut(s) | 1229, 1377, 1498, 1834, 2295, 2431, 2794 |
| BspDI | ATCGAT | 1 cut(s) | 95 |
| BspEI | TCCGGA | 1 cut(s) | 346 |
| BspHI | TCATGA | 1 cut(s) | 1467 |
| BspMAI | CTGCAG | 2 cut(s) | 45, 1413 |
| BspMI | ACCTGC | 2 cut(s) | 48, 457 |
| BspPI | GGATC | 8 cut(s) | 100, 237, 2171, 2184, 2366, 2379, 2525, 3030 |
| BspT107I | GGYRCC | 2 cut(s) | 299, 2167 |
| BsrDI | GCAATG | 3 cut(s) | 846, 1275, 2127 |
| BsrFI | RCCGGY | 1 cut(s) | 328 |
| BsrI | ACTGG | 8 cut(s) | 503, 539, 1822, 1881, 2039, 2089, 2203, 2917 |
| BssAI | RCCGGY | 1 cut(s) | 328 |
| BssECI | CCNNGG | 9 cut(s) | 169, 414, 540, 643, 758, 1861, 2180, 2367, 2641 |
| BssNI | GRCGYC | 1 cut(s) | 300 |
| BssT1I | CCWWGG | 4 cut(s) | 758, 1861, 2367, 2641 |
| Bst2UI | CCWGG | 3 cut(s) | 53, 1758, 2029 |
| Bst4CI | ACNGT | 8 cut(s) | 218, 1776, 2218, 2245, 2411, 2416, 3012, 3119 |
| Bst6I | CTCTTC | 1 cut(s) | 2720 |
| BstACI | GRCGYC | 1 cut(s) | 300 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 67, 1817 |
| BstC8I | GCNNGC | 5 cut(s) | 330, 446, 621, 1351, 1701 |
| BstDSI | CCRYGG | 3 cut(s) | 414, 1861, 2180 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 44 |
| BstF5I | GGATG | 4 cut(s) | 644, 768, 1195, 1978 |
| BstH2I | RGCGCY | 1 cut(s) | 303 |
| BstHHI | GCGC | 2 cut(s) | 302, 392 |
| BstMAI | GTCTC | 4 cut(s) | 107, 163, 2115, 2267 |
| BstMCI | CGRYCG | 1 cut(s) | 24 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 67, 1199, 1280, 1753, 1817, 3092 |
| BstNI | CCWGG | 3 cut(s) | 53, 1758, 2029 |
| BstNSI | RCATGY | 2 cut(s) | 487, 2521 |
| BstSCI | CCNGG | 8 cut(s) | 51, 169, 302, 374, 539, 540, 1756, 2027 |
| BstSFI | CTRYAG | 3 cut(s) | 41, 1409, 2415 |
| BstV1I | GCAGC | 3 cut(s) | 1423, 1728, 2468 |
| BstV2I | GAAGAC | 4 cut(s) | 23, 118, 1118, 1157 |
| BstX2I | RGATCY | 4 cut(s) | 183, 2176, 2371, 3022 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1829 |
| BstYI | RGATCY | 4 cut(s) | 183, 2176, 2371, 3022 |
| Bsu15I | ATCGAT | 1 cut(s) | 95 |
| Bsu36I | CCTNAGG | 2 cut(s) | 2439, 2802 |
| BsuRI | GGCC | 5 cut(s) | 51, 174, 328, 363, 2366 |
| BsuTUI | ATCGAT | 1 cut(s) | 95 |
| BtgI | CCRYGG | 3 cut(s) | 414, 1861, 2180 |
| BtgZI | GCGATG | 1 cut(s) | 134 |
| BtsCI | GGATG | 4 cut(s) | 644, 768, 1195, 1978 |
| BtsI | GCAGTG | 1 cut(s) | 232 |
| BtsIMutI | CAGTG | 5 cut(s) | 232, 289, 814, 2887, 3008 |
| BveI | ACCTGC | 2 cut(s) | 48, 457 |
| Cac8I | GCNNGC | 5 cut(s) | 330, 446, 621, 1351, 1701 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2980 |
| CciI | TCATGA | 1 cut(s) | 1467 |
| CfoI | GCGC | 2 cut(s) | 302, 392 |
| Cfr10I | RCCGGY | 1 cut(s) | 328 |
| Cfr13I | GGNCC | 7 cut(s) | 49, 145, 326, 537, 682, 1297, 1572 |
| Cfr9I | CCCGGG | 1 cut(s) | 540 |
| ClaI | ATCGAT | 1 cut(s) | 95 |
| CseI | GACGC | 2 cut(s) | 124, 268 |
| CsiI | ACCWGGT | 1 cut(s) | 2027 |
| Csp6I | GTAC | 8 cut(s) | 37, 460, 1227, 1605, 1884, 2232, 2412, 2873 |
| CviAII | CATG | 8 cut(s) | 238, 484, 577, 872, 905, 1468, 1862, 2518 |
| CviQI | GTAC | 8 cut(s) | 37, 460, 1227, 1605, 1884, 2232, 2412, 2873 |
| DinI | GGCGCC | 1 cut(s) | 301 |
| DraI | TTTAAA | 1 cut(s) | 742 |
| DraIII | CACNNNGTG | 1 cut(s) | 961 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 143 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 143 |
| EaeI | YGGCCR | 1 cut(s) | 361 |
| Eam1104I | CTCTTC | 1 cut(s) | 2720 |
| EarI | CTCTTC | 1 cut(s) | 2720 |
| Ecl136II | GAGCTC | 1 cut(s) | 2749 |
| Eco130I | CCWWGG | 4 cut(s) | 758, 1861, 2367, 2641 |
| Eco24I | GRGCYC | 2 cut(s) | 2751, 2996 |
| Eco32I | GATATC | 1 cut(s) | 971 |
| Eco47I | GGWCC | 5 cut(s) | 145, 537, 682, 1297, 1572 |
| Eco53kI | GAGCTC | 1 cut(s) | 2749 |
| Eco57I | CTGAAG | 2 cut(s) | 2498, 2841 |
| Eco81I | CCTNAGG | 2 cut(s) | 2439, 2802 |
| Eco88I | CYCGRG | 2 cut(s) | 540, 1523 |
| EcoICRI | GAGCTC | 1 cut(s) | 2749 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 44 |
| EcoO109I | RGGNCCY | 1 cut(s) | 682 |
| EcoRI | GAATTC | 4 cut(s) | 419, 2571, 2582, 2603 |
| EcoRII | CCWGG | 3 cut(s) | 51, 1756, 2027 |
| EcoRV | GATATC | 1 cut(s) | 971 |
| EcoT14I | CCWWGG | 4 cut(s) | 758, 1861, 2367, 2641 |
| EcoT22I | ATGCAT | 1 cut(s) | 3059 |
| EcoT38I | GRGCYC | 2 cut(s) | 2751, 2996 |
| EgeI | GGCGCC | 1 cut(s) | 301 |
| EheI | GGCGCC | 1 cut(s) | 301 |
| ErhI | CCWWGG | 4 cut(s) | 758, 1861, 2367, 2641 |
| Esp3I | CGTCTC | 2 cut(s) | 107, 163 |
| FaeI | CATG | 8 cut(s) | 241, 487, 580, 875, 908, 1471, 1865, 2521 |
| FaqI | GGGAC | 9 cut(s) | 152, 668, 1226, 1310, 1382, 1637, 1972, 2087, 2266 |
| FatI | CATG | 8 cut(s) | 237, 483, 576, 871, 904, 1467, 1861, 2517 |
| FbaI | TGATCA | 2 cut(s) | 1464, 1474 |
| FblI | GTMKAC | 1 cut(s) | 2430 |
| Fnu4HI | GCNGC | 5 cut(s) | 210, 1412, 1742, 1745, 2457 |
| FokI | GGATG | 4 cut(s) | 651, 775, 1202, 1985 |
| FriOI | GRGCYC | 2 cut(s) | 2751, 2996 |
| Fsp4HI | GCNGC | 5 cut(s) | 210, 1412, 1742, 1745, 2457 |
| FspBI | CTAG | 3 cut(s) | 1199, 1640, 2426 |
| GlaI | GCGC | 2 cut(s) | 301, 391 |
| GluI | GCNGC | 5 cut(s) | 210, 1412, 1742, 1745, 2457 |
| GsuI | CTGGAG | 4 cut(s) | 1205, 2547, 2832, 3086 |
| HaeII | RGCGCY | 1 cut(s) | 303 |
| HaeIII | GGCC | 5 cut(s) | 51, 174, 328, 363, 2366 |
| HapII | CCGG | 6 cut(s) | 171, 303, 329, 347, 375, 541 |
| HgaI | GACGC | 2 cut(s) | 124, 268 |
| HhaI | GCGC | 2 cut(s) | 302, 392 |
| Hin1I | GRCGYC | 1 cut(s) | 300 |
| Hin1II | CATG | 8 cut(s) | 241, 487, 580, 875, 908, 1471, 1865, 2521 |
| Hin6I | GCGC | 2 cut(s) | 300, 390 |
| HinP1I | GCGC | 2 cut(s) | 300, 390 |
| HincII | GTYRAC | 1 cut(s) | 2922 |
| HindII | GTYRAC | 1 cut(s) | 2922 |
| HindIII | AAGCTT | 3 cut(s) | 1533, 1546, 2672 |
| HpaII | CCGG | 6 cut(s) | 171, 303, 329, 347, 375, 541 |
| HphI | GGTGA | 9 cut(s) | 214, 247, 364, 391, 556, 1568, 1877, 2168, 2582 |
| Hpy166II | GTNNAC | 8 cut(s) | 205, 221, 474, 1073, 1227, 2431, 2845, 2922 |
| Hpy188I | TCNGA | 8 cut(s) | 92, 499, 647, 1219, 1461, 1488, 1843, 2293 |
| Hpy8I | GTNNAC | 8 cut(s) | 205, 221, 474, 1073, 1227, 2431, 2845, 2922 |
| Hpy99I | CGWCG | 4 cut(s) | 140, 266, 317, 443 |
| HpyAV | CCTTC | 9 cut(s) | 40, 328, 652, 1203, 1534, 1610, 1946, 2066, 3013 |
| HpyCH4III | ACNGT | 8 cut(s) | 218, 1776, 2218, 2245, 2411, 2416, 3012, 3119 |
| HpyCH4IV | ACGT | 6 cut(s) | 165, 264, 1662, 1678, 1926, 2918 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 67, 1199, 1280, 1753, 1817, 3092 |
| HpySE526I | ACGT | 6 cut(s) | 165, 264, 1662, 1678, 1926, 2918 |
| Hsp92I | GRCGYC | 1 cut(s) | 300 |
| Hsp92II | CATG | 8 cut(s) | 241, 487, 580, 875, 908, 1471, 1865, 2521 |
| HspAI | GCGC | 2 cut(s) | 300, 390 |
| KasI | GGCGCC | 1 cut(s) | 299 |
| KflI | GGGWCCC | 1 cut(s) | 682 |
| Kpn2I | TCCGGA | 1 cut(s) | 346 |
| KroI | GCCGGC | 1 cut(s) | 328 |
| KroNI | GCCGGC | 1 cut(s) | 330 |
| Ksp22I | TGATCA | 2 cut(s) | 1464, 1474 |
| LmnI | GCTCC | 6 cut(s) | 58, 158, 2091, 2293, 2797, 2813 |
| Lsp1109I | GCAGC | 3 cut(s) | 1423, 1728, 2468 |
| LweI | GCATC | 5 cut(s) | 629, 1180, 1182, 1465, 1549 |
| MabI | ACCWGGT | 1 cut(s) | 2027 |
| MaeI | CTAG | 3 cut(s) | 1199, 1640, 2426 |
| MaeII | ACGT | 6 cut(s) | 165, 264, 1662, 1678, 1926, 2918 |
| MaeIII | GTNAC | 5 cut(s) | 253, 544, 883, 1919, 1964 |
| MflI | RGATCY | 4 cut(s) | 183, 2176, 2371, 3022 |
| MhlI | GDGCHC | 3 cut(s) | 63, 2751, 2996 |
| MlsI | TGGCCA | 1 cut(s) | 363 |
| MluNI | TGGCCA | 1 cut(s) | 363 |
| Mly113I | GGCGCC | 1 cut(s) | 300 |
| MlyI | GAGTC | 4 cut(s) | 236, 1208, 1954, 2348 |
| MmeI | TCCRAC | 2 cut(s) | 2069, 2628 |
| Mox20I | TGGCCA | 1 cut(s) | 363 |
| Mph1103I | ATGCAT | 1 cut(s) | 3059 |
| MroI | TCCGGA | 1 cut(s) | 346 |
| MroNI | GCCGGC | 1 cut(s) | 328 |
| MroXI | GAANNNNTTC | 4 cut(s) | 1125, 2571, 2586, 2762 |
| MscI | TGGCCA | 1 cut(s) | 363 |
| MseI | TTAA | 9 cut(s) | 573, 741, 1091, 1445, 1578, 1788, 1929, 2151, 2547 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1863, 1895, 2403 |
| Msp20I | TGGCCA | 1 cut(s) | 363 |
| MspI | CCGG | 6 cut(s) | 171, 303, 329, 347, 375, 541 |
| MspR9I | CCNGG | 8 cut(s) | 53, 171, 304, 376, 541, 542, 1758, 2029 |
| Mva1269I | GAATGC | 1 cut(s) | 1846 |
| MvaI | CCWGG | 3 cut(s) | 53, 1758, 2029 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 67, 1199, 1280, 1753, 1817, 3092 |
| NaeI | GCCGGC | 1 cut(s) | 330 |
| NarI | GGCGCC | 1 cut(s) | 300 |
| NciI | CCSGG | 5 cut(s) | 171, 304, 376, 541, 542 |
| NcoI | CCATGG | 1 cut(s) | 1861 |
| NgoMIV | GCCGGC | 1 cut(s) | 328 |
| NlaIII | CATG | 8 cut(s) | 241, 487, 580, 875, 908, 1471, 1865, 2521 |
| NmuCI | GTSAC | 3 cut(s) | 253, 544, 883 |
| NsiI | ATGCAT | 1 cut(s) | 3059 |
| NspI | RCATGY | 2 cut(s) | 487, 2521 |
| OliI | CACNNNNGTG | 1 cut(s) | 1863 |
| PagI | TCATGA | 1 cut(s) | 1467 |
| PaqCI | CACCTGC | 1 cut(s) | 457 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 135 |
| PctI | GAATGC | 1 cut(s) | 1846 |
| PdiI | GCCGGC | 1 cut(s) | 330 |
| PdmI | GAANNNNTTC | 4 cut(s) | 1125, 2571, 2586, 2762 |
| PkrI | GCNGC | 5 cut(s) | 211, 1413, 1743, 1746, 2458 |
| Ple19I | CGATCG | 1 cut(s) | 24 |
| PleI | GAGTC | 4 cut(s) | 236, 1208, 1954, 2347 |
| PluTI | GGCGCC | 1 cut(s) | 303 |
| PpsI | GAGTC | 4 cut(s) | 236, 1208, 1954, 2347 |
| PpuMI | RGGWCCY | 1 cut(s) | 682 |
| PsiI | TTATAA | 3 cut(s) | 291, 2112, 2325 |
| Psp124BI | GAGCTC | 1 cut(s) | 2751 |
| Psp1406I | AACGTT | 2 cut(s) | 1926, 2918 |
| Psp5II | RGGWCCY | 1 cut(s) | 682 |
| Psp6I | CCWGG | 3 cut(s) | 51, 1756, 2027 |
| PspGI | CCWGG | 3 cut(s) | 51, 1756, 2027 |
| PspPI | GGNCC | 7 cut(s) | 49, 145, 326, 537, 682, 1297, 1572 |
| PspPPI | RGGWCCY | 1 cut(s) | 682 |
| PstI | CTGCAG | 2 cut(s) | 45, 1413 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2980 |
| PsuI | RGATCY | 4 cut(s) | 183, 2176, 2371, 3022 |
| PvuI | CGATCG | 1 cut(s) | 24 |
| RsaI | GTAC | 8 cut(s) | 38, 461, 1228, 1606, 1885, 2233, 2413, 2874 |
| RsaNI | GTAC | 8 cut(s) | 37, 460, 1227, 1605, 1884, 2232, 2412, 2873 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1863, 1895, 2403 |
| SacI | GAGCTC | 1 cut(s) | 2751 |
| SaqAI | TTAA | 9 cut(s) | 573, 741, 1091, 1445, 1578, 1788, 1929, 2151, 2547 |
| SatI | GCNGC | 5 cut(s) | 210, 1412, 1742, 1745, 2457 |
| Sau96I | GGNCC | 7 cut(s) | 49, 145, 326, 537, 682, 1297, 1572 |
| ScaI | AGTACT | 3 cut(s) | 1606, 1885, 2413 |
| SchI | GAGTC | 4 cut(s) | 236, 1208, 1954, 2348 |
| ScrFI | CCNGG | 8 cut(s) | 53, 171, 304, 376, 541, 542, 1758, 2029 |
| SduI | GDGCHC | 3 cut(s) | 63, 2751, 2996 |
| SexAI | ACCWGGT | 1 cut(s) | 2027 |
| SfaNI | GCATC | 5 cut(s) | 629, 1180, 1182, 1465, 1549 |
| SfcI | CTRYAG | 3 cut(s) | 41, 1409, 2415 |
| SfoI | GGCGCC | 1 cut(s) | 301 |
| SinI | GGWCC | 5 cut(s) | 145, 537, 682, 1297, 1572 |
| SmaI | CCCGGG | 1 cut(s) | 542 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1863, 1895, 2403 |
| SmlI | CTYRAG | 5 cut(s) | 947, 1549, 2576, 2744, 2951 |
| SmoI | CTYRAG | 5 cut(s) | 947, 1549, 2576, 2744, 2951 |
| SsiI | CCGC | 4 cut(s) | 210, 487, 1744, 2143 |
| SspDI | GGCGCC | 1 cut(s) | 299 |
| SspI | AATATT | 1 cut(s) | 406 |
| SspMI | CTAG | 3 cut(s) | 1199, 1640, 2426 |
| SstI | GAGCTC | 1 cut(s) | 2751 |
| StyD4I | CCNGG | 8 cut(s) | 51, 169, 302, 374, 539, 540, 1756, 2027 |
| StyI | CCWWGG | 4 cut(s) | 758, 1861, 2367, 2641 |
| TaaI | ACNGT | 8 cut(s) | 218, 1776, 2218, 2245, 2411, 2416, 3012, 3119 |
| TaiI | ACGT | 6 cut(s) | 168, 267, 1665, 1681, 1929, 2921 |
| TatI | WGTACW | 4 cut(s) | 459, 1604, 1883, 2411 |
| TauI | GCSGC | 2 cut(s) | 212, 1747 |
| Tru1I | TTAA | 9 cut(s) | 573, 741, 1091, 1445, 1578, 1788, 1929, 2151, 2547 |
| Tru9I | TTAA | 9 cut(s) | 573, 741, 1091, 1445, 1578, 1788, 1929, 2151, 2547 |
| TscAI | CASTG | 5 cut(s) | 239, 289, 814, 2887, 3015 |
| TseFI | GTSAC | 3 cut(s) | 253, 544, 883 |
| TseI | GCWGC | 3 cut(s) | 1411, 1741, 2456 |
| Tsp45I | GTSAC | 3 cut(s) | 253, 544, 883 |
| TspGWI | ACGGA | 7 cut(s) | 125, 145, 238, 431, 1172, 2169, 2294 |
| TspMI | CCCGGG | 1 cut(s) | 540 |
| TspRI | CASTG | 5 cut(s) | 239, 289, 814, 2887, 3015 |
| VpaK11BI | GGWCC | 5 cut(s) | 145, 537, 682, 1297, 1572 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 44 |
| XapI | RAATTY | 9 cut(s) | 419, 569, 737, 2330, 2421, 2483, 2571, 2582, 2603 |
| XbaI | TCTAGA | 1 cut(s) | 1639 |
| XceI | RCATGY | 2 cut(s) | 487, 2521 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2178 |
| XmaI | CCCGGG | 1 cut(s) | 540 |
| XmiI | GTMKAC | 1 cut(s) | 2430 |
| XmnI | GAANNNNTTC | 4 cut(s) | 1125, 2571, 2586, 2762 |
| XspI | CTAG | 3 cut(s) | 1199, 1640, 2426 |
| ZrmI | AGTACT | 3 cut(s) | 1606, 1885, 2413 |
| Zsp2I | ATGCAT | 1 cut(s) | 3059 |