RchiOBHm_Chr1g0380661

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001532
Physical Location & Seq
Reverse (-)
66244275 .. 66246275
2001 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60392

Sequence Viewer

Length: 1029 bp
ATGTCAACTTCAAGTCTCGCACTAAACGGCGTTGTTTCCATGAAGGGTTGGGAAAGGTATGGTGTTTACCCACTGGTAGGTGAATTGCTGAACGTGAGAGAAGCTGGCTTCTTTGAGGTCATGTATAATCCGATTTTCAAGACGATTATGAAGGCTCTCGGACTTGAGCTAGATGAAGAATTTACCAGTACCGAATCTTTGAGATATGGCCGGGTCATTATTATGACTGCTGAACAGGATCATTATGGTAGTTACATCAAAGGGTTGTTGATCAATTTTCTTCACATGTTCTGGCCATCATTGCTCAAGCTTCCGTCATTTTTGGCCGAGCTTATAGCTCCTCTTACCATCAAGGCGACAAAAGAGAGTGAGGAAGGGAAAGAAAAACGTGTATTCTATTCCCTGCAAGAGTATGAAGCTTGGAGGGAAAGTTTGAGGGGCAATGCGAGTGGCTGGGCCATTGAGAAGGATGTAGCATGTGTTTATGATGAAGGGAAGGATTATTTTGAAAATCTTTTAATGCAGAAGAAAGATTTTGTTTGGGTAGATGAACGTGATGATGAGGCAATTGAGCTTGCTTTCAGCAAGAAGTTTGAGGTGGGAAGTTTGCAGTCTGGTATGCATCTTGATCATGAGGAGAAACTTGTAACTTATAGCGACTTTGTTGACAAGGAGCTTATACCCTTTTCCATGCTTGCTGTTCTGCAACGCTCAATTCCGTCAATGGTTGATGGCTTGAAGCCTCATCAAAGAAAGGTTCTATTCAGCTCTTTTAAGAGGGACGACTTTATCAAGGAAGAAATAGTTGCCAACTTTGCTAATTATGTGGCTGAACACTCGGCTTGTCCTGTGTGCGAGCCCTCAGCGTACCACCATGATGAGCAGAGTCTTGACAGTACAATCATTGAAATGGCCCAGGCTTATGTTGGCAGCAACAACATTAATCTTCTTTTACCAAGAGGTCTATTTGGAAGCCGCCGATTGGTGAGATACCAAGATTTCATCATGCAATGCAACTTTATTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 map01524
Pfam Domains
Protein Families
DNA_gyraseB DNA_topoisoIV HATPase_c TOPRIM_C Toprim

Protein Analysis

342

Amino Acids

39.31

Weight (kDa)

5.08

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOPRIM_C PF16898 117 - 232 2.2e-19 C-terminal associated domain of TOPRIM
DNA_topoisoIV PF00521 237 - 326 1.2e-20 DNA gyrase/topoisomerase IV, subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 976
AclWI GGATC 1 cut(s) 246
AcoI YGGCCR 3 cut(s) 208, 293, 324
AcsI RAATTY 1 cut(s) 179
AfaI GTAC 3 cut(s) 190, 869, 898
AfiI CCNNNNNNNGG 2 cut(s) 77, 982
AflIII ACRYGT 2 cut(s) 285, 388
AgsI TTSAA 5 cut(s) 12, 139, 509, 739, 908
AjnI CCWGG 1 cut(s) 915
AluBI AGCT 9 cut(s) 104, 169, 310, 331, 338, 419, 574, 676, 768
AluI AGCT 9 cut(s) 104, 169, 310, 331, 338, 419, 574, 676, 768
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 1 cut(s) 246
AoxI GGCC 5 cut(s) 208, 293, 324, 456, 912
ApeKI GCWGC 1 cut(s) 930
ApoI RAATTY 1 cut(s) 179
AseI ATTAAT 1 cut(s) 942
AspS9I GGNCC 2 cut(s) 456, 913
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 92, 997
BalI TGGCCA 1 cut(s) 295
BanII GRGCYC 1 cut(s) 861
BbvCI CCTCAGC 1 cut(s) 862
BbvI GCAGC 1 cut(s) 942
BccI CCATC 3 cut(s) 304, 356, 725
BceAI ACGGC 1 cut(s) 43
BciT130I CCWGG 1 cut(s) 917
BclI TGATCA 2 cut(s) 270, 628
BcnI CCSGG 1 cut(s) 212
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 1 cut(s) 170
BisI GCNGC 2 cut(s) 931, 976
BlsI GCNGC 2 cut(s) 932, 977
Bme1390I CCNGG 2 cut(s) 212, 917
BmgT120I GGNCC 2 cut(s) 456, 913
BmrFI CCNGG 2 cut(s) 212, 917
BmsI GCATC 1 cut(s) 631
Bpu10I CCTNAGC 1 cut(s) 862
BpuEI CTTGAG 2 cut(s) 185, 290
BpuMI CCSGG 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 915
BsaXI ACNNNNNCTCC 2 cut(s) 415, 445
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 982
Bse1I ACTGG 2 cut(s) 78, 186
Bse3DI GCAATG 3 cut(s) 299, 448, 1016
BseBI CCWGG 1 cut(s) 917
BseDI CCNNGG 1 cut(s) 915
BseGI GGATG 1 cut(s) 475
BseLI CCNNNNNNNGG 2 cut(s) 77, 982
BseMI GCAATG 3 cut(s) 299, 448, 1016
BseMII CTCAG 1 cut(s) 876
BseNI ACTGG 2 cut(s) 78, 186
BseRI GAGGAG 2 cut(s) 330, 650
BseXI GCAGC 1 cut(s) 942
BseYI CCCAGC 1 cut(s) 453
BshFI GGCC 5 cut(s) 210, 295, 326, 458, 914
BsiSI CCGG 1 cut(s) 211
BslFI GGGAC 1 cut(s) 794
BslI CCNNNNNNNGG 2 cut(s) 77, 982
BsmAI GTCTC 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 794
BsnI GGCC 5 cut(s) 210, 295, 326, 458, 914
Bsp1286I GDGCHC 1 cut(s) 861
Bsp143I GATC 3 cut(s) 238, 270, 628
BspACI CCGC 1 cut(s) 976
BspANI GGCC 5 cut(s) 210, 295, 326, 458, 914
BspCNI CTCAG 1 cut(s) 875
BspHI TCATGA 1 cut(s) 631
BspPI GGATC 1 cut(s) 246
BsrDI GCAATG 3 cut(s) 299, 448, 1016
BsrI ACTGG 2 cut(s) 78, 186
BssECI CCNNGG 1 cut(s) 915
BssMI GATC 3 cut(s) 238, 270, 628
Bst2UI CCWGG 1 cut(s) 917
Bst4CI ACNGT 1 cut(s) 896
BstC8I GCNNGC 4 cut(s) 106, 576, 696, 857
BstDEI CTNAG 1 cut(s) 862
BstF5I GGATG 1 cut(s) 475
BstKTI GATC 3 cut(s) 241, 273, 631
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 3 cut(s) 238, 270, 628
BstMWI GCNNNNNNNGC 2 cut(s) 301, 815
BstNI CCWGG 1 cut(s) 917
BstNSI RCATGY 2 cut(s) 289, 480
BstSCI CCNGG 2 cut(s) 210, 915
BstV1I GCAGC 1 cut(s) 942
BsuRI GGCC 5 cut(s) 210, 295, 326, 458, 914
BtsCI GGATG 1 cut(s) 475
BtsIMutI CAGTG 1 cut(s) 71
Cac8I GCNNGC 4 cut(s) 106, 576, 696, 857
CciI TCATGA 1 cut(s) 631
Cfr13I GGNCC 2 cut(s) 456, 913
Csp6I GTAC 3 cut(s) 189, 868, 897
CspCI CAANNNNNGTGG 2 cut(s) 430, 465
CviAII CATG 8 cut(s) 40, 121, 286, 477, 632, 691, 875, 1006
CviQI GTAC 3 cut(s) 189, 868, 897
DdeI CTNAG 1 cut(s) 862
DpnI GATC 3 cut(s) 240, 272, 630
DpnII GATC 3 cut(s) 238, 270, 628
EaeI YGGCCR 3 cut(s) 208, 293, 324
Eco24I GRGCYC 1 cut(s) 861
EcoRII CCWGG 1 cut(s) 915
EcoT22I ATGCAT 1 cut(s) 624
EcoT38I GRGCYC 1 cut(s) 861
FaeI CATG 8 cut(s) 43, 124, 289, 480, 635, 694, 878, 1009
FaqI GGGAC 1 cut(s) 794
FatI CATG 8 cut(s) 39, 120, 285, 476, 631, 690, 874, 1005
FbaI TGATCA 2 cut(s) 270, 628
Fnu4HI GCNGC 2 cut(s) 931, 976
FokI GGATG 1 cut(s) 482
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 2 cut(s) 931, 976
FspBI CTAG 1 cut(s) 170
GluI GCNGC 2 cut(s) 931, 976
GsaI CCCAGC 1 cut(s) 457
HaeIII GGCC 5 cut(s) 210, 295, 326, 458, 914
HapII CCGG 1 cut(s) 211
Hin1II CATG 8 cut(s) 43, 124, 289, 480, 635, 694, 878, 1009
HincII GTYRAC 2 cut(s) 6, 667
HindII GTYRAC 2 cut(s) 6, 667
HindIII AAGCTT 2 cut(s) 308, 417
HinfI GANTC 2 cut(s) 194, 886
HpaII CCGG 1 cut(s) 211
HphI GGTGA 2 cut(s) 92, 997
Hpy166II GTNNAC 3 cut(s) 6, 67, 667
Hpy188I TCNGA 2 cut(s) 132, 161
Hpy188III TCNNGA 4 cut(s) 139, 626, 632, 890
Hpy8I GTNNAC 3 cut(s) 6, 67, 667
HpyAV CCTTC 6 cut(s) 37, 145, 368, 460, 485, 490
HpyCH4III ACNGT 1 cut(s) 896
HpyCH4IV ACGT 3 cut(s) 93, 388, 553
HpyCH4V TGCA 7 cut(s) 406, 523, 610, 622, 706, 1009, 1014
HpyF10VI GCNNNNNNNGC 2 cut(s) 301, 815
HpyF3I CTNAG 1 cut(s) 862
HpySE526I ACGT 3 cut(s) 93, 388, 553
Hsp92II CATG 8 cut(s) 43, 124, 289, 480, 635, 694, 878, 1009
Ksp22I TGATCA 2 cut(s) 270, 628
Kzo9I GATC 3 cut(s) 238, 270, 628
LmnI GCTCC 2 cut(s) 343, 673
Lsp1109I GCAGC 1 cut(s) 942
LweI GCATC 1 cut(s) 631
MaeI CTAG 1 cut(s) 170
MaeII ACGT 3 cut(s) 93, 388, 553
MaeIII GTNAC 2 cut(s) 251, 646
MalI GATC 3 cut(s) 240, 272, 630
MboI GATC 3 cut(s) 238, 270, 628
MboII GAAGA 5 cut(s) 188, 272, 538, 809, 938
MfeI CAATTG 1 cut(s) 567
MhlI GDGCHC 1 cut(s) 861
MlsI TGGCCA 1 cut(s) 295
MluCI AATT 6 cut(s) 83, 179, 274, 567, 714, 820
MluNI TGGCCA 1 cut(s) 295
MlyI GAGTC 1 cut(s) 895
Mox20I TGGCCA 1 cut(s) 295
Mph1103I ATGCAT 1 cut(s) 624
MscI TGGCCA 1 cut(s) 295
MseI TTAA 3 cut(s) 518, 774, 942
MslI CAYNNNNRTG 3 cut(s) 221, 876, 908
Msp20I TGGCCA 1 cut(s) 295
MspI CCGG 1 cut(s) 211
MspR9I CCNGG 2 cut(s) 212, 917
MunI CAATTG 1 cut(s) 567
MvaI CCWGG 1 cut(s) 917
MwoI GCNNNNNNNGC 2 cut(s) 301, 815
NciI CCSGG 1 cut(s) 212
NdeII GATC 3 cut(s) 238, 270, 628
NlaIII CATG 8 cut(s) 43, 124, 289, 480, 635, 694, 878, 1009
NmeAIII GCCGAG 2 cut(s) 352, 818
NsiI ATGCAT 1 cut(s) 624
NspI RCATGY 2 cut(s) 289, 480
PagI TCATGA 1 cut(s) 631
PciI ACATGT 1 cut(s) 285
PfeI GAWTC 1 cut(s) 194
PkrI GCNGC 2 cut(s) 932, 977
PleI GAGTC 1 cut(s) 894
PpsI GAGTC 1 cut(s) 894
PscI ACATGT 1 cut(s) 285
PshBI ATTAAT 1 cut(s) 942
Psp6I CCWGG 1 cut(s) 915
PspFI CCCAGC 1 cut(s) 453
PspGI CCWGG 1 cut(s) 915
PspPI GGNCC 2 cut(s) 456, 913
RsaI GTAC 3 cut(s) 190, 869, 898
RsaNI GTAC 3 cut(s) 189, 868, 897
RseI CAYNNNNRTG 3 cut(s) 221, 876, 908
SaqAI TTAA 3 cut(s) 518, 774, 942
SatI GCNGC 2 cut(s) 931, 976
Sau3AI GATC 3 cut(s) 238, 270, 628
Sau96I GGNCC 2 cut(s) 456, 913
SchI GAGTC 1 cut(s) 895
ScrFI CCNGG 2 cut(s) 212, 917
SduI GDGCHC 1 cut(s) 861
SfaNI GCATC 1 cut(s) 631
SmiMI CAYNNNNRTG 3 cut(s) 221, 876, 908
SmlI CTYRAG 2 cut(s) 164, 305
SmoI CTYRAG 2 cut(s) 164, 305
Sse9I AATT 6 cut(s) 83, 179, 274, 567, 714, 820
SsiI CCGC 1 cut(s) 976
SspMI CTAG 1 cut(s) 170
StyD4I CCNGG 2 cut(s) 210, 915
TaaI ACNGT 1 cut(s) 896
TaiI ACGT 3 cut(s) 96, 391, 556
TasI AATT 6 cut(s) 83, 179, 274, 567, 714, 820
TatI WGTACW 1 cut(s) 896
TauI GCSGC 1 cut(s) 978
TfiI GAWTC 1 cut(s) 194
Tru1I TTAA 3 cut(s) 518, 774, 942
Tru9I TTAA 3 cut(s) 518, 774, 942
TscAI CASTG 1 cut(s) 78
TseI GCWGC 1 cut(s) 930
TspDTI ATGAA 7 cut(s) 56, 164, 189, 429, 504, 564, 991
TspGWI ACGGA 2 cut(s) 303, 708
TspRI CASTG 1 cut(s) 78
VspI ATTAAT 1 cut(s) 942
XapI RAATTY 1 cut(s) 179
XceI RCATGY 2 cut(s) 289, 480
XcmI CCANNNNNNNNNTGG 1 cut(s) 923
XspI CTAG 1 cut(s) 170
Zsp2I ATGCAT 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.