Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| DNA_gyraseB | PF00204 | 249 - 361 | 1.1e-21 | DNA gyrase B |
| Toprim | PF01751 | 425 - 523 | 3.4e-07 | Toprim domain |
| TOPRIM_C | PF16898 | 539 - 666 | 2.9e-47 | C-terminal associated domain of TOPRIM |
| DNA_topoisoIV | PF00521 | 669 - 1128 | 2.8e-116 | DNA gyrase/topoisomerase IV, subunit A |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 2469, 2682 |
| AarI | CACCTGC | 1 cut(s) | 499 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 143 |
| Acc36I | ACCTGC | 4 cut(s) | 499, 3322, 3602, 3643 |
| AccB1I | GGYRCC | 1 cut(s) | 431 |
| AciI | CCGC | 9 cut(s) | 118, 210, 429, 477, 1722, 1994, 2500, 3662, 3755 |
| AcoI | YGGCCR | 1 cut(s) | 361 |
| AcuI | CTGAAG | 5 cut(s) | 62, 3198, 3733, 3812, 3816 |
| AcvI | CACGTG | 1 cut(s) | 1003 |
| AcyI | GRCGYC | 1 cut(s) | 432 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 1069, 1364, 2149, 3004, 3611 |
| AgeI | ACCGGT | 1 cut(s) | 410 |
| AhlI | ACTAGT | 1 cut(s) | 374 |
| AjnI | CCWGG | 5 cut(s) | 51, 1912, 2038, 2384, 3817 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 2717, 2749, 2972, 3004 |
| AleI | CACNNNNGTG | 1 cut(s) | 2145 |
| Alw21I | GWGCWC | 5 cut(s) | 63, 1002, 2278, 2453, 3108 |
| Alw26I | GTCTC | 4 cut(s) | 163, 2472, 2624, 3720 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3212 |
| Ama87I | CYCGRG | 1 cut(s) | 582 |
| AoxI | GGCC | 7 cut(s) | 172, 361, 405, 1577, 2721, 3752, 3835 |
| AseI | ATTAAT | 1 cut(s) | 2211 |
| AsiGI | ACCGGT | 1 cut(s) | 410 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 1167, 2928, 2943, 3119 |
| AspLEI | GCGC | 3 cut(s) | 434, 3481, 3644 |
| AspS9I | GGNCC | 9 cut(s) | 145, 234, 579, 724, 1339, 1578, 1728, 2670, 3695 |
| AsuC2I | CCSGG | 5 cut(s) | 171, 583, 584, 3414, 3606 |
| AvaI | CYCGRG | 1 cut(s) | 582 |
| AvaII | GGWCC | 8 cut(s) | 145, 234, 579, 724, 1339, 1728, 2670, 3695 |
| AxyI | CCTNAGG | 1 cut(s) | 2796 |
| BamHI | GGATCC | 2 cut(s) | 2533, 2728 |
| BanI | GGYRCC | 1 cut(s) | 431 |
| BanII | GRGCYC | 2 cut(s) | 2453, 3108 |
| BauI | CACGAG | 1 cut(s) | 1967 |
| BbrPI | CACGTG | 1 cut(s) | 1003 |
| BbsI | GAAGAC | 3 cut(s) | 118, 1160, 1199 |
| Bbv12I | GWGCWC | 5 cut(s) | 63, 1002, 2278, 2453, 3108 |
| BceAI | ACGGC | 2 cut(s) | 439, 499 |
| BciT130I | CCWGG | 5 cut(s) | 53, 1914, 2040, 2386, 3819 |
| BclI | TGATCA | 4 cut(s) | 1506, 1516, 2761, 2901 |
| BcnI | CCSGG | 5 cut(s) | 171, 583, 584, 3414, 3606 |
| BcoDI | GTCTC | 4 cut(s) | 163, 2472, 2624, 3720 |
| BcuI | ACTAGT | 1 cut(s) | 374 |
| BfaI | CTAG | 6 cut(s) | 375, 783, 1241, 1796, 2783, 3705 |
| BfmI | CTRYAG | 2 cut(s) | 1451, 2772 |
| BfoI | RGCGCY | 2 cut(s) | 435, 3645 |
| BfuAI | ACCTGC | 4 cut(s) | 499, 3322, 3602, 3643 |
| BglII | AGATCT | 1 cut(s) | 183 |
| BmcAI | AGTACT | 4 cut(s) | 1739, 1762, 2167, 2770 |
| Bme18I | GGWCC | 8 cut(s) | 145, 234, 579, 724, 1339, 1728, 2670, 3695 |
| BmeT110I | CYCGRG | 1 cut(s) | 582 |
| BmgT120I | GGNCC | 9 cut(s) | 145, 234, 579, 724, 1339, 1578, 1728, 2670, 3695 |
| BmrI | ACTGGG | 1 cut(s) | 2098 |
| BmsI | GCATC | 8 cut(s) | 70, 288, 407, 671, 1222, 1507, 3376, 3792 |
| BmuI | ACTGGG | 1 cut(s) | 2098 |
| BpiI | GAAGAC | 3 cut(s) | 118, 1160, 1199 |
| BpmI | CTGGAG | 4 cut(s) | 1247, 2467, 2714, 2904 |
| Bpu10I | CCTNAGC | 2 cut(s) | 996, 1299 |
| BpuEI | CTTGAG | 8 cut(s) | 233, 1690, 2954, 3020, 3122, 3287, 3425, 3702 |
| BpuMI | CCSGG | 5 cut(s) | 171, 583, 584, 3414, 3606 |
| Bsa29I | ATCGAT | 1 cut(s) | 95 |
| BsaAI | YACGTR | 1 cut(s) | 1003 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1236, 1521 |
| BsaHI | GRCGYC | 1 cut(s) | 432 |
| BsaJI | CCNNGG | 9 cut(s) | 169, 237, 582, 685, 2143, 2537, 2724, 3413, 3604 |
| BsaWI | WCCGGW | 2 cut(s) | 410, 3269 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1517, 1547 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 1069, 1364, 2149, 3004, 3611 |
| Bse118I | RCCGGY | 1 cut(s) | 410 |
| Bse21I | CCTNAGG | 1 cut(s) | 2796 |
| Bse3DI | GCAATG | 4 cut(s) | 888, 1317, 1567, 3555 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1236, 1521 |
| BseBI | CCWGG | 5 cut(s) | 53, 1914, 2040, 2386, 3819 |
| BseCI | ATCGAT | 1 cut(s) | 95 |
| BseDI | CCNNGG | 9 cut(s) | 169, 237, 582, 685, 2143, 2537, 2724, 3413, 3604 |
| BseGI | GGATG | 7 cut(s) | 422, 686, 810, 1237, 2335, 2457, 3565 |
| BseJI | GATNNNNATC | 2 cut(s) | 1236, 1521 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 1069, 1364, 2149, 3004, 3611 |
| BseMI | GCAATG | 4 cut(s) | 888, 1317, 1567, 3555 |
| BseMII | CTCAG | 7 cut(s) | 987, 1655, 1671, 2653, 2787, 3219, 3276 |
| BseRI | GAGGAG | 7 cut(s) | 544, 1895, 1985, 2813, 3422, 3862, 3865 |
| BseYI | CCCAGC | 1 cut(s) | 1316 |
| BshFI | GGCC | 7 cut(s) | 174, 363, 407, 1579, 2723, 3754, 3837 |
| BshNI | GGYRCC | 1 cut(s) | 431 |
| BshTI | ACCGGT | 1 cut(s) | 410 |
| BshVI | ATCGAT | 1 cut(s) | 95 |
| BsiHKAI | GWGCWC | 5 cut(s) | 63, 1002, 2278, 2453, 3108 |
| BsiHKCI | CYCGRG | 1 cut(s) | 582 |
| BsiSI | CCGG | 7 cut(s) | 171, 347, 411, 583, 3270, 3413, 3606 |
| BslFI | GGGAC | 8 cut(s) | 314, 710, 1268, 1352, 1793, 2254, 2452, 3905 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 1069, 1364, 2149, 3004, 3611 |
| BsmAI | GTCTC | 4 cut(s) | 163, 2472, 2624, 3720 |
| BsmBI | CGTCTC | 1 cut(s) | 163 |
| BsmFI | GGGAC | 8 cut(s) | 314, 710, 1268, 1352, 1793, 2254, 2452, 3905 |
| BsmI | GAATGC | 2 cut(s) | 2128, 3481 |
| BsnI | GGCC | 7 cut(s) | 174, 363, 407, 1579, 2723, 3754, 3837 |
| BsoBI | CYCGRG | 1 cut(s) | 582 |
| Bsp1286I | GDGCHC | 5 cut(s) | 63, 1002, 2278, 2453, 3108 |
| Bsp19I | CCATGG | 2 cut(s) | 237, 2143 |
| BspACI | CCGC | 9 cut(s) | 118, 210, 429, 477, 1722, 1994, 2500, 3662, 3755 |
| BspANI | GGCC | 7 cut(s) | 174, 363, 407, 1579, 2723, 3754, 3837 |
| BspCNI | CTCAG | 7 cut(s) | 988, 1654, 1672, 2652, 2788, 3218, 3277 |
| BspDI | ATCGAT | 1 cut(s) | 95 |
| BspHI | TCATGA | 1 cut(s) | 1509 |
| BspMAI | CTGCAG | 1 cut(s) | 1455 |
| BspMI | ACCTGC | 4 cut(s) | 499, 3322, 3602, 3643 |
| BspQI | GCTCTTC | 2 cut(s) | 41, 3752 |
| BspT107I | GGYRCC | 1 cut(s) | 431 |
| BsrDI | GCAATG | 4 cut(s) | 888, 1317, 1567, 3555 |
| BsrFI | RCCGGY | 1 cut(s) | 410 |
| BssAI | RCCGGY | 1 cut(s) | 410 |
| BssECI | CCNNGG | 9 cut(s) | 169, 237, 582, 685, 2143, 2537, 2724, 3413, 3604 |
| BssNI | GRCGYC | 1 cut(s) | 432 |
| BssSI | CACGAG | 1 cut(s) | 1967 |
| BssT1I | CCWWGG | 5 cut(s) | 237, 685, 2143, 2537, 2724 |
| Bst2BI | CACGAG | 1 cut(s) | 1967 |
| Bst2UI | CCWGG | 5 cut(s) | 53, 1914, 2040, 2386, 3819 |
| Bst6I | CTCTTC | 7 cut(s) | 14, 41, 1001, 3077, 3192, 3683, 3752 |
| BstACI | GRCGYC | 1 cut(s) | 432 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 67, 426, 2099, 2159 |
| BstBAI | YACGTR | 1 cut(s) | 1003 |
| BstC8I | GCNNGC | 6 cut(s) | 488, 1323, 1393, 1720, 1845, 3640 |
| BstDSI | CCRYGG | 2 cut(s) | 237, 2143 |
| BstEII | GGTNACC | 1 cut(s) | 253 |
| BstF5I | GGATG | 7 cut(s) | 422, 686, 810, 1237, 2335, 2457, 3565 |
| BstH2I | RGCGCY | 2 cut(s) | 435, 3645 |
| BstHHI | GCGC | 3 cut(s) | 434, 3481, 3644 |
| BstMAI | GTCTC | 4 cut(s) | 163, 2472, 2624, 3720 |
| BstNI | CCWGG | 5 cut(s) | 53, 1914, 2040, 2386, 3819 |
| BstNSI | RCATGY | 1 cut(s) | 2878 |
| BstPI | GGTNACC | 1 cut(s) | 253 |
| BstSFI | CTRYAG | 2 cut(s) | 1451, 2772 |
| BstV2I | GAAGAC | 3 cut(s) | 118, 1160, 1199 |
| BstX2I | RGATCY | 5 cut(s) | 183, 1996, 2533, 2728, 3379 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2111 |
| BstYI | RGATCY | 5 cut(s) | 183, 1996, 2533, 2728, 3379 |
| Bsu15I | ATCGAT | 1 cut(s) | 95 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2796 |
| BsuRI | GGCC | 7 cut(s) | 174, 363, 407, 1579, 2723, 3754, 3837 |
| BsuTUI | ATCGAT | 1 cut(s) | 95 |
| BtgI | CCRYGG | 2 cut(s) | 237, 2143 |
| BtgZI | GCGATG | 1 cut(s) | 134 |
| BtsCI | GGATG | 7 cut(s) | 422, 686, 810, 1237, 2335, 2457, 3565 |
| BtsIMutI | CAGTG | 4 cut(s) | 289, 332, 1960, 2405 |
| BveI | ACCTGC | 4 cut(s) | 499, 3322, 3602, 3643 |
| Cac8I | GCNNGC | 6 cut(s) | 488, 1323, 1393, 1720, 1845, 3640 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3212 |
| CciI | TCATGA | 1 cut(s) | 1509 |
| CfoI | GCGC | 3 cut(s) | 434, 3481, 3644 |
| Cfr10I | RCCGGY | 1 cut(s) | 410 |
| Cfr13I | GGNCC | 9 cut(s) | 145, 234, 579, 724, 1339, 1578, 1728, 2670, 3695 |
| Cfr9I | CCCGGG | 1 cut(s) | 582 |
| ClaI | ATCGAT | 1 cut(s) | 95 |
| CseI | GACGC | 1 cut(s) | 3794 |
| CsiI | ACCWGGT | 1 cut(s) | 2384 |
| CspAI | ACCGGT | 1 cut(s) | 410 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1110, 1145, 3132, 3167 |
| DinI | GGCGCC | 1 cut(s) | 433 |
| DraI | TTTAAA | 1 cut(s) | 1618 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 143 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 143 |
| EaeI | YGGCCR | 1 cut(s) | 361 |
| Eam1104I | CTCTTC | 7 cut(s) | 14, 41, 1001, 3077, 3192, 3683, 3752 |
| EarI | CTCTTC | 7 cut(s) | 14, 41, 1001, 3077, 3192, 3683, 3752 |
| EciI | GGCGGA | 1 cut(s) | 2009 |
| Ecl136II | GAGCTC | 2 cut(s) | 2451, 3106 |
| Eco130I | CCWWGG | 5 cut(s) | 237, 685, 2143, 2537, 2724 |
| Eco24I | GRGCYC | 2 cut(s) | 2453, 3108 |
| Eco32I | GATATC | 1 cut(s) | 537 |
| Eco47I | GGWCC | 8 cut(s) | 145, 234, 579, 724, 1339, 1728, 2670, 3695 |
| Eco53kI | GAGCTC | 2 cut(s) | 2451, 3106 |
| Eco57I | CTGAAG | 5 cut(s) | 62, 3198, 3733, 3812, 3816 |
| Eco72I | CACGTG | 1 cut(s) | 1003 |
| Eco81I | CCTNAGG | 1 cut(s) | 2796 |
| Eco88I | CYCGRG | 1 cut(s) | 582 |
| Eco91I | GGTNACC | 1 cut(s) | 253 |
| EcoICRI | GAGCTC | 2 cut(s) | 2451, 3106 |
| EcoO109I | RGGNCCY | 1 cut(s) | 724 |
| EcoO65I | GGTNACC | 1 cut(s) | 253 |
| EcoRI | GAATTC | 2 cut(s) | 2939, 2960 |
| EcoRII | CCWGG | 5 cut(s) | 51, 1912, 2038, 2384, 3817 |
| EcoRV | GATATC | 1 cut(s) | 537 |
| EcoT14I | CCWWGG | 5 cut(s) | 237, 685, 2143, 2537, 2724 |
| EcoT38I | GRGCYC | 2 cut(s) | 2453, 3108 |
| EgeI | GGCGCC | 1 cut(s) | 433 |
| EheI | GGCGCC | 1 cut(s) | 433 |
| ErhI | CCWWGG | 5 cut(s) | 237, 685, 2143, 2537, 2724 |
| Esp3I | CGTCTC | 1 cut(s) | 163 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 1061, 1093, 1256, 1288, 1799, 1831, 2063, 2095 |
| FaqI | GGGAC | 8 cut(s) | 314, 710, 1268, 1352, 1793, 2254, 2452, 3905 |
| FbaI | TGATCA | 4 cut(s) | 1506, 1516, 2761, 2901 |
| FokI | GGATG | 7 cut(s) | 429, 693, 817, 1244, 2342, 2444, 3572 |
| FriOI | GRGCYC | 2 cut(s) | 2453, 3108 |
| FspBI | CTAG | 6 cut(s) | 375, 783, 1241, 1796, 2783, 3705 |
| GlaI | GCGC | 3 cut(s) | 433, 3480, 3643 |
| GsaI | CCCAGC | 1 cut(s) | 1320 |
| GsuI | CTGGAG | 4 cut(s) | 1247, 2467, 2714, 2904 |
| HaeII | RGCGCY | 2 cut(s) | 435, 3645 |
| HaeIII | GGCC | 7 cut(s) | 174, 363, 407, 1579, 2723, 3754, 3837 |
| HapII | CCGG | 7 cut(s) | 171, 347, 411, 583, 3270, 3413, 3606 |
| HgaI | GACGC | 1 cut(s) | 3794 |
| HhaI | GCGC | 3 cut(s) | 434, 3481, 3644 |
| Hin1I | GRCGYC | 1 cut(s) | 432 |
| Hin6I | GCGC | 3 cut(s) | 432, 3479, 3642 |
| HinP1I | GCGC | 3 cut(s) | 432, 3479, 3642 |
| HincII | GTYRAC | 1 cut(s) | 3267 |
| HindII | GTYRAC | 1 cut(s) | 3267 |
| HindIII | AAGCTT | 2 cut(s) | 1689, 1940 |
| HpaII | CCGG | 7 cut(s) | 171, 347, 411, 583, 3270, 3413, 3606 |
| Hpy166II | GTNNAC | 7 cut(s) | 205, 1115, 1269, 1966, 2670, 2788, 3267 |
| Hpy8I | GTNNAC | 7 cut(s) | 205, 1115, 1269, 1966, 2670, 2788, 3267 |
| Hpy99I | CGWCG | 4 cut(s) | 140, 266, 392, 485 |
| HpyCH4IV | ACGT | 4 cut(s) | 264, 924, 1002, 3504 |
| HpySE526I | ACGT | 4 cut(s) | 264, 924, 1002, 3504 |
| Hsp92I | GRCGYC | 1 cut(s) | 432 |
| HspAI | GCGC | 3 cut(s) | 432, 3479, 3642 |
| KasI | GGCGCC | 1 cut(s) | 431 |
| KflI | GGGWCCC | 1 cut(s) | 724 |
| Ksp22I | TGATCA | 4 cut(s) | 1506, 1516, 2761, 2901 |
| LguI | GCTCTTC | 2 cut(s) | 41, 3752 |
| LmnI | GCTCC | 9 cut(s) | 58, 158, 1538, 1972, 2448, 3111, 3154, 3409, 3608 |
| LweI | GCATC | 8 cut(s) | 70, 288, 407, 671, 1222, 1507, 3376, 3792 |
| MabI | ACCWGGT | 1 cut(s) | 2384 |
| MaeI | CTAG | 6 cut(s) | 375, 783, 1241, 1796, 2783, 3705 |
| MaeII | ACGT | 4 cut(s) | 264, 924, 1002, 3504 |
| MaeIII | GTNAC | 9 cut(s) | 253, 380, 586, 851, 925, 2201, 2246, 3813, 3871 |
| MfeI | CAATTG | 1 cut(s) | 1848 |
| MflI | RGATCY | 5 cut(s) | 183, 1996, 2533, 2728, 3379 |
| MhlI | GDGCHC | 5 cut(s) | 63, 1002, 2278, 2453, 3108 |
| Mly113I | GGCGCC | 1 cut(s) | 432 |
| MmeI | TCCRAC | 3 cut(s) | 2970, 2985, 3133 |
| MroXI | GAANNNNTTC | 4 cut(s) | 1167, 2928, 2943, 3119 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1347, 2145, 2177, 2271 |
| MspA1I | CMGCKG | 2 cut(s) | 3599, 3662 |
| MspI | CCGG | 7 cut(s) | 171, 347, 411, 583, 3270, 3413, 3606 |
| MunI | CAATTG | 1 cut(s) | 1848 |
| Mva1269I | GAATGC | 2 cut(s) | 2128, 3481 |
| MvaI | CCWGG | 5 cut(s) | 53, 1914, 2040, 2386, 3819 |
| NarI | GGCGCC | 1 cut(s) | 432 |
| NciI | CCSGG | 5 cut(s) | 171, 583, 584, 3414, 3606 |
| NcoI | CCATGG | 2 cut(s) | 237, 2143 |
| NmeAIII | GCCGAG | 1 cut(s) | 2272 |
| NmuCI | GTSAC | 5 cut(s) | 253, 586, 851, 925, 3871 |
| NspI | RCATGY | 1 cut(s) | 2878 |
| OliI | CACNNNNGTG | 1 cut(s) | 2145 |
| PagI | TCATGA | 1 cut(s) | 1509 |
| PaqCI | CACCTGC | 1 cut(s) | 499 |
| PciSI | GCTCTTC | 2 cut(s) | 41, 3752 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 135, 477 |
| PctI | GAATGC | 2 cut(s) | 2128, 3481 |
| PdmI | GAANNNNTTC | 4 cut(s) | 1167, 2928, 2943, 3119 |
| PfeI | GAWTC | 9 cut(s) | 196, 307, 1058, 1292, 1441, 1792, 2582, 3036, 3234 |
| PinAI | ACCGGT | 1 cut(s) | 410 |
| PluTI | GGCGCC | 1 cut(s) | 435 |
| PmaCI | CACGTG | 1 cut(s) | 1003 |
| PmlI | CACGTG | 1 cut(s) | 1003 |
| Ppu21I | YACGTR | 1 cut(s) | 1003 |
| PpuMI | RGGWCCY | 1 cut(s) | 724 |
| PshBI | ATTAAT | 1 cut(s) | 2211 |
| PsiI | TTATAA | 2 cut(s) | 2469, 2682 |
| Psp124BI | GAGCTC | 2 cut(s) | 2453, 3108 |
| Psp5II | RGGWCCY | 1 cut(s) | 724 |
| Psp6I | CCWGG | 5 cut(s) | 51, 1912, 2038, 2384, 3817 |
| PspCI | CACGTG | 1 cut(s) | 1003 |
| PspEI | GGTNACC | 1 cut(s) | 253 |
| PspFI | CCCAGC | 1 cut(s) | 1316 |
| PspGI | CCWGG | 5 cut(s) | 51, 1912, 2038, 2384, 3817 |
| PspPI | GGNCC | 9 cut(s) | 145, 234, 579, 724, 1339, 1578, 1728, 2670, 3695 |
| PspPPI | RGGWCCY | 1 cut(s) | 724 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 3265, 3297 |
| PstI | CTGCAG | 1 cut(s) | 1455 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3212 |
| PsuI | RGATCY | 5 cut(s) | 183, 1996, 2533, 2728, 3379 |
| PvuII | CAGCTG | 1 cut(s) | 3599 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1347, 2145, 2177, 2271 |
| SacI | GAGCTC | 2 cut(s) | 2453, 3108 |
| SapI | GCTCTTC | 2 cut(s) | 41, 3752 |
| Sau96I | GGNCC | 9 cut(s) | 145, 234, 579, 724, 1339, 1578, 1728, 2670, 3695 |
| ScaI | AGTACT | 4 cut(s) | 1739, 1762, 2167, 2770 |
| SduI | GDGCHC | 5 cut(s) | 63, 1002, 2278, 2453, 3108 |
| SexAI | ACCWGGT | 1 cut(s) | 2384 |
| SfaNI | GCATC | 8 cut(s) | 70, 288, 407, 671, 1222, 1507, 3376, 3792 |
| SfcI | CTRYAG | 2 cut(s) | 1451, 2772 |
| SfoI | GGCGCC | 1 cut(s) | 433 |
| SgrAI | CRCCGGYG | 1 cut(s) | 410 |
| SinI | GGWCC | 8 cut(s) | 145, 234, 579, 724, 1339, 1728, 2670, 3695 |
| SmaI | CCCGGG | 1 cut(s) | 584 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1347, 2145, 2177, 2271 |
| SmlI | CTYRAG | 8 cut(s) | 248, 1705, 2933, 2999, 3101, 3302, 3404, 3717 |
| SmoI | CTYRAG | 8 cut(s) | 248, 1705, 2933, 2999, 3101, 3302, 3404, 3717 |
| SpeI | ACTAGT | 1 cut(s) | 374 |
| SsiI | CCGC | 9 cut(s) | 118, 210, 429, 477, 1722, 1994, 2500, 3662, 3755 |
| SspDI | GGCGCC | 1 cut(s) | 431 |
| SspI | AATATT | 2 cut(s) | 1432, 2209 |
| SspMI | CTAG | 6 cut(s) | 375, 783, 1241, 1796, 2783, 3705 |
| SstI | GAGCTC | 2 cut(s) | 2453, 3108 |
| StyI | CCWWGG | 5 cut(s) | 237, 685, 2143, 2537, 2724 |
| TaiI | ACGT | 4 cut(s) | 267, 927, 1005, 3507 |
| TaqII | GACCGA | 1 cut(s) | 222 |
| TatI | WGTACW | 5 cut(s) | 501, 1737, 1760, 2165, 2768 |
| TauI | GCSGC | 5 cut(s) | 212, 432, 1725, 3665, 3757 |
| TfiI | GAWTC | 9 cut(s) | 196, 307, 1058, 1292, 1441, 1792, 2582, 3036, 3234 |
| TscAI | CASTG | 4 cut(s) | 289, 332, 1960, 2412 |
| TseFI | GTSAC | 5 cut(s) | 253, 586, 851, 925, 3871 |
| Tsp45I | GTSAC | 5 cut(s) | 253, 586, 851, 925, 3871 |
| TspGWI | ACGGA | 4 cut(s) | 145, 238, 2651, 3127 |
| TspMI | CCCGGG | 1 cut(s) | 582 |
| TspRI | CASTG | 4 cut(s) | 289, 332, 1960, 2412 |
| VpaK11BI | GGWCC | 8 cut(s) | 145, 234, 579, 724, 1339, 1728, 2670, 3695 |
| VspI | ATTAAT | 1 cut(s) | 2211 |
| XbaI | TCTAGA | 2 cut(s) | 782, 1795 |
| XceI | RCATGY | 1 cut(s) | 2878 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 2535, 2664, 3366, 3547 |
| XmaI | CCCGGG | 1 cut(s) | 582 |
| XmnI | GAANNNNTTC | 4 cut(s) | 1167, 2928, 2943, 3119 |
| XspI | CTAG | 6 cut(s) | 375, 783, 1241, 1796, 2783, 3705 |
| ZrmI | AGTACT | 4 cut(s) | 1739, 1762, 2167, 2770 |