Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 620, 675 |
| AatII | GACGTC | 1 cut(s) | 251 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 722, 1001 |
| AccI | GTMKAC | 2 cut(s) | 477, 1038 |
| AccII | CGCG | 1 cut(s) | 1146 |
| AciI | CCGC | 4 cut(s) | 198, 380, 458, 1144 |
| AclI | AACGTT | 1 cut(s) | 936 |
| AcsI | RAATTY | 4 cut(s) | 60, 68, 445, 745 |
| AcuI | CTGAAG | 1 cut(s) | 156 |
| AcyI | GRCGYC | 1 cut(s) | 248 |
| AfaI | GTAC | 1 cut(s) | 135 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 15, 143, 722, 1001 |
| AflIII | ACRYGT | 3 cut(s) | 534, 886, 1095 |
| AgsI | TTSAA | 5 cut(s) | 115, 661, 1114, 1184, 1239 |
| AluBI | AGCT | 6 cut(s) | 308, 422, 506, 551, 569, 696 |
| AluI | AGCT | 6 cut(s) | 308, 422, 506, 551, 569, 696 |
| Alw21I | GWGCWC | 3 cut(s) | 508, 796, 1208 |
| Alw26I | GTCTC | 4 cut(s) | 81, 255, 758, 1075 |
| Alw44I | GTGCAC | 1 cut(s) | 792 |
| AoxI | GGCC | 2 cut(s) | 3, 1137 |
| ApaLI | GTGCAC | 1 cut(s) | 792 |
| ApeKI | GCWGC | 3 cut(s) | 313, 709, 989 |
| ApoI | RAATTY | 4 cut(s) | 60, 68, 445, 745 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 218, 250, 1082, 1114 |
| AsuC2I | CCSGG | 1 cut(s) | 545 |
| AsuHPI | GGTGA | 2 cut(s) | 68, 1310 |
| BaeGI | GKGCMC | 1 cut(s) | 796 |
| BanII | GRGCYC | 1 cut(s) | 508 |
| BbsI | GAAGAC | 1 cut(s) | 738 |
| Bbv12I | GWGCWC | 3 cut(s) | 508, 796, 1208 |
| BbvI | GCAGC | 3 cut(s) | 300, 696, 976 |
| BccI | CCATC | 3 cut(s) | 422, 494, 1129 |
| BceAI | ACGGC | 1 cut(s) | 1057 |
| BcnI | CCSGG | 1 cut(s) | 545 |
| BcoDI | GTCTC | 4 cut(s) | 81, 255, 758, 1075 |
| BfaI | CTAG | 6 cut(s) | 189, 566, 608, 767, 909, 926 |
| BisI | GCNGC | 3 cut(s) | 314, 710, 990 |
| BlsI | GCNGC | 3 cut(s) | 315, 711, 991 |
| Bme1390I | CCNGG | 1 cut(s) | 545 |
| BmrFI | CCNGG | 1 cut(s) | 545 |
| BmrI | ACTGGG | 2 cut(s) | 732, 1253 |
| BmsI | GCATC | 3 cut(s) | 721, 1204, 1291 |
| BmuI | ACTGGG | 2 cut(s) | 732, 1253 |
| BpiI | GAAGAC | 1 cut(s) | 738 |
| BpmI | CTGGAG | 1 cut(s) | 978 |
| BpuEI | CTTGAG | 5 cut(s) | 31, 830, 904, 1241, 1252 |
| BpuMI | CCSGG | 1 cut(s) | 545 |
| BsaHI | GRCGYC | 1 cut(s) | 248 |
| BsaI | GGTCTC | 1 cut(s) | 1075 |
| BsaJI | CCNNGG | 1 cut(s) | 544 |
| BsaWI | WCCGGW | 1 cut(s) | 53 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 35, 65 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 15, 143, 722, 1001 |
| Bse1I | ACTGG | 4 cut(s) | 727, 801, 886, 1248 |
| Bse3DI | GCAATG | 2 cut(s) | 771, 862 |
| BseDI | CCNNGG | 1 cut(s) | 544 |
| BseGI | GGATG | 1 cut(s) | 712 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 15, 143, 722, 1001 |
| BseMI | GCAATG | 2 cut(s) | 771, 862 |
| BseNI | ACTGG | 4 cut(s) | 727, 801, 886, 1248 |
| BseRI | GAGGAG | 1 cut(s) | 716 |
| BseSI | GKGCMC | 1 cut(s) | 796 |
| BseXI | GCAGC | 3 cut(s) | 300, 696, 976 |
| BseYI | CCCAGC | 1 cut(s) | 1196 |
| BsgI | GTGCAG | 1 cut(s) | 150 |
| Bsh1236I | CGCG | 1 cut(s) | 1146 |
| BshFI | GGCC | 2 cut(s) | 5, 1139 |
| BsiHKAI | GWGCWC | 3 cut(s) | 508, 796, 1208 |
| BsiSI | CCGG | 2 cut(s) | 54, 544 |
| BslFI | GGGAC | 3 cut(s) | 596, 739, 988 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 15, 143, 722, 1001 |
| BsmAI | GTCTC | 4 cut(s) | 81, 255, 758, 1075 |
| BsmBI | CGTCTC | 1 cut(s) | 255 |
| BsmFI | GGGAC | 3 cut(s) | 596, 739, 988 |
| BsmI | GAATGC | 4 cut(s) | 287, 1050, 1232, 1323 |
| BsnI | GGCC | 2 cut(s) | 5, 1139 |
| Bso31I | GGTCTC | 1 cut(s) | 1075 |
| Bsp1286I | GDGCHC | 3 cut(s) | 508, 796, 1208 |
| Bsp143I | GATC | 2 cut(s) | 211, 841 |
| BspACI | CCGC | 4 cut(s) | 198, 380, 458, 1144 |
| BspANI | GGCC | 2 cut(s) | 5, 1139 |
| BspFNI | CGCG | 1 cut(s) | 1146 |
| BspTNI | GGTCTC | 1 cut(s) | 1075 |
| BsrDI | GCAATG | 2 cut(s) | 771, 862 |
| BsrI | ACTGG | 4 cut(s) | 727, 801, 886, 1248 |
| BssECI | CCNNGG | 1 cut(s) | 544 |
| BssMI | GATC | 2 cut(s) | 211, 841 |
| BssNI | GRCGYC | 1 cut(s) | 248 |
| Bst4CI | ACNGT | 2 cut(s) | 979, 1109 |
| Bst6I | CTCTTC | 2 cut(s) | 300, 1073 |
| BstACI | GRCGYC | 1 cut(s) | 248 |
| BstC8I | GCNNGC | 2 cut(s) | 1148, 1261 |
| BstDEI | CTNAG | 1 cut(s) | 697 |
| BstF5I | GGATG | 1 cut(s) | 712 |
| BstFNI | CGCG | 1 cut(s) | 1146 |
| BstKTI | GATC | 2 cut(s) | 214, 844 |
| BstMAI | GTCTC | 4 cut(s) | 81, 255, 758, 1075 |
| BstMBI | GATC | 2 cut(s) | 211, 841 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1197 |
| BstNSI | RCATGY | 5 cut(s) | 538, 630, 890, 1099, 1263 |
| BstSCI | CCNGG | 1 cut(s) | 543 |
| BstSLI | GKGCMC | 1 cut(s) | 796 |
| BstUI | CGCG | 1 cut(s) | 1146 |
| BstV1I | GCAGC | 3 cut(s) | 300, 696, 976 |
| BstV2I | GAAGAC | 1 cut(s) | 738 |
| BsuRI | GGCC | 2 cut(s) | 5, 1139 |
| BtsCI | GGATG | 1 cut(s) | 712 |
| BtsIMutI | CAGTG | 2 cut(s) | 794, 1086 |
| Cac8I | GCNNGC | 2 cut(s) | 1148, 1261 |
| CseI | GACGC | 1 cut(s) | 743 |
| Csp6I | GTAC | 1 cut(s) | 134 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 1321 |
| CviAII | CATG | 7 cut(s) | 259, 535, 627, 682, 887, 1096, 1260 |
| CviQI | GTAC | 1 cut(s) | 134 |
| DdeI | CTNAG | 1 cut(s) | 697 |
| DpnI | GATC | 2 cut(s) | 213, 843 |
| DpnII | GATC | 2 cut(s) | 211, 841 |
| Eam1104I | CTCTTC | 2 cut(s) | 300, 1073 |
| EarI | CTCTTC | 2 cut(s) | 300, 1073 |
| Ecl136II | GAGCTC | 1 cut(s) | 506 |
| Eco24I | GRGCYC | 1 cut(s) | 508 |
| Eco31I | GGTCTC | 1 cut(s) | 1075 |
| Eco53kI | GAGCTC | 1 cut(s) | 506 |
| Eco57I | CTGAAG | 1 cut(s) | 156 |
| EcoICRI | GAGCTC | 1 cut(s) | 506 |
| EcoT38I | GRGCYC | 1 cut(s) | 508 |
| Esp3I | CGTCTC | 1 cut(s) | 255 |
| FaeI | CATG | 7 cut(s) | 262, 538, 630, 685, 890, 1099, 1263 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 154, 186, 1124, 1156 |
| FaqI | GGGAC | 3 cut(s) | 596, 739, 988 |
| FatI | CATG | 7 cut(s) | 258, 534, 626, 681, 886, 1095, 1259 |
| FauI | CCCGC | 1 cut(s) | 451 |
| FauNDI | CATATG | 1 cut(s) | 1027 |
| FblI | GTMKAC | 2 cut(s) | 477, 1038 |
| Fnu4HI | GCNGC | 3 cut(s) | 314, 710, 990 |
| FokI | GGATG | 1 cut(s) | 699 |
| FriOI | GRGCYC | 1 cut(s) | 508 |
| Fsp4HI | GCNGC | 3 cut(s) | 314, 710, 990 |
| FspBI | CTAG | 6 cut(s) | 189, 566, 608, 767, 909, 926 |
| GluI | GCNGC | 3 cut(s) | 314, 710, 990 |
| GsaI | CCCAGC | 1 cut(s) | 1200 |
| GsuI | CTGGAG | 1 cut(s) | 978 |
| HaeIII | GGCC | 2 cut(s) | 5, 1139 |
| HapII | CCGG | 2 cut(s) | 54, 544 |
| HgaI | GACGC | 1 cut(s) | 743 |
| Hin1I | GRCGYC | 1 cut(s) | 248 |
| Hin1II | CATG | 7 cut(s) | 262, 538, 630, 685, 890, 1099, 1263 |
| HincII | GTYRAC | 2 cut(s) | 640, 1023 |
| HindII | GTYRAC | 2 cut(s) | 640, 1023 |
| HinfI | GANTC | 6 cut(s) | 19, 242, 383, 912, 1169, 1271 |
| HpaII | CCGG | 2 cut(s) | 54, 544 |
| HphI | GGTGA | 2 cut(s) | 68, 1310 |
| Hpy166II | GTNNAC | 7 cut(s) | 478, 640, 794, 1023, 1039, 1071, 1105 |
| Hpy188I | TCNGA | 6 cut(s) | 182, 211, 587, 596, 762, 1312 |
| Hpy188III | TCNNGA | 7 cut(s) | 65, 415, 613, 921, 942, 995, 1220 |
| Hpy8I | GTNNAC | 7 cut(s) | 478, 640, 794, 1023, 1039, 1071, 1105 |
| Hpy99I | CGWCG | 1 cut(s) | 250 |
| HpyAV | CCTTC | 4 cut(s) | 230, 517, 1127, 1150 |
| HpyCH4III | ACNGT | 2 cut(s) | 979, 1109 |
| HpyCH4IV | ACGT | 3 cut(s) | 248, 783, 936 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1197 |
| HpyF3I | CTNAG | 1 cut(s) | 697 |
| HpySE526I | ACGT | 3 cut(s) | 248, 783, 936 |
| Hsp92I | GRCGYC | 1 cut(s) | 248 |
| Hsp92II | CATG | 7 cut(s) | 262, 538, 630, 685, 890, 1099, 1263 |
| Kzo9I | GATC | 2 cut(s) | 211, 841 |
| LmnI | GCTCC | 4 cut(s) | 503, 548, 693, 997 |
| Lsp1109I | GCAGC | 3 cut(s) | 300, 696, 976 |
| LweI | GCATC | 3 cut(s) | 721, 1204, 1291 |
| MaeI | CTAG | 6 cut(s) | 189, 566, 608, 767, 909, 926 |
| MaeII | ACGT | 3 cut(s) | 248, 783, 936 |
| MaeIII | GTNAC | 1 cut(s) | 537 |
| MalI | GATC | 2 cut(s) | 213, 843 |
| MboI | GATC | 2 cut(s) | 211, 841 |
| MboII | GAAGA | 7 cut(s) | 287, 509, 743, 754, 797, 1090, 1144 |
| MhlI | GDGCHC | 3 cut(s) | 508, 796, 1208 |
| MluCI | AATT | 6 cut(s) | 60, 68, 138, 445, 745, 1248 |
| MlyI | GAGTC | 1 cut(s) | 377 |
| MmeI | TCCRAC | 3 cut(s) | 160, 740, 1321 |
| MseI | TTAA | 5 cut(s) | 102, 141, 153, 531, 749 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1100 |
| MspI | CCGG | 2 cut(s) | 54, 544 |
| MspR9I | CCNGG | 1 cut(s) | 545 |
| Mva1269I | GAATGC | 4 cut(s) | 287, 1050, 1232, 1323 |
| MvnI | CGCG | 1 cut(s) | 1146 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1197 |
| NciI | CCSGG | 1 cut(s) | 545 |
| NdeI | CATATG | 1 cut(s) | 1027 |
| NdeII | GATC | 2 cut(s) | 211, 841 |
| NlaIII | CATG | 7 cut(s) | 262, 538, 630, 685, 890, 1099, 1263 |
| NmeAIII | GCCGAG | 2 cut(s) | 471, 876 |
| NspI | RCATGY | 5 cut(s) | 538, 630, 890, 1099, 1263 |
| PaeI | GCATGC | 1 cut(s) | 1263 |
| PciI | ACATGT | 3 cut(s) | 534, 886, 1095 |
| PctI | GAATGC | 4 cut(s) | 287, 1050, 1232, 1323 |
| PfeI | GAWTC | 5 cut(s) | 19, 242, 912, 1169, 1271 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 722, 1001 |
| PkrI | GCNGC | 3 cut(s) | 315, 711, 991 |
| PleI | GAGTC | 1 cut(s) | 377 |
| PpsI | GAGTC | 1 cut(s) | 377 |
| PscI | ACATGT | 3 cut(s) | 534, 886, 1095 |
| PsiI | TTATAA | 2 cut(s) | 620, 675 |
| Psp124BI | GAGCTC | 1 cut(s) | 508 |
| Psp1406I | AACGTT | 1 cut(s) | 936 |
| PspFI | CCCAGC | 1 cut(s) | 1196 |
| RsaI | GTAC | 1 cut(s) | 135 |
| RsaNI | GTAC | 1 cut(s) | 134 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1100 |
| SacI | GAGCTC | 1 cut(s) | 508 |
| SaqAI | TTAA | 5 cut(s) | 102, 141, 153, 531, 749 |
| SatI | GCNGC | 3 cut(s) | 314, 710, 990 |
| Sau3AI | GATC | 2 cut(s) | 211, 841 |
| SchI | GAGTC | 1 cut(s) | 377 |
| ScrFI | CCNGG | 1 cut(s) | 545 |
| SduI | GDGCHC | 3 cut(s) | 508, 796, 1208 |
| SfaNI | GCATC | 3 cut(s) | 721, 1204, 1291 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1100 |
| SmlI | CTYRAG | 5 cut(s) | 10, 845, 919, 1220, 1231 |
| SmoI | CTYRAG | 5 cut(s) | 10, 845, 919, 1220, 1231 |
| SphI | GCATGC | 1 cut(s) | 1263 |
| Sse9I | AATT | 6 cut(s) | 60, 68, 138, 445, 745, 1248 |
| SsiI | CCGC | 4 cut(s) | 198, 380, 458, 1144 |
| SspMI | CTAG | 6 cut(s) | 189, 566, 608, 767, 909, 926 |
| SstI | GAGCTC | 1 cut(s) | 508 |
| StyD4I | CCNGG | 1 cut(s) | 543 |
| TaaI | ACNGT | 2 cut(s) | 979, 1109 |
| TaiI | ACGT | 3 cut(s) | 251, 786, 939 |
| TaqI | TCGA | 6 cut(s) | 22, 38, 245, 416, 632, 1327 |
| TaqII | GACCGA | 1 cut(s) | 1299 |
| TasI | AATT | 6 cut(s) | 60, 68, 138, 445, 745, 1248 |
| TfiI | GAWTC | 5 cut(s) | 19, 242, 912, 1169, 1271 |
| Tru1I | TTAA | 5 cut(s) | 102, 141, 153, 531, 749 |
| Tru9I | TTAA | 5 cut(s) | 102, 141, 153, 531, 749 |
| TscAI | CASTG | 2 cut(s) | 801, 1093 |
| TseI | GCWGC | 3 cut(s) | 313, 709, 989 |
| TspDTI | ATGAA | 6 cut(s) | 275, 354, 670, 797, 824, 1161 |
| TspRI | CASTG | 2 cut(s) | 801, 1093 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 722, 1001 |
| VneI | GTGCAC | 1 cut(s) | 792 |
| XapI | RAATTY | 4 cut(s) | 60, 68, 445, 745 |
| XceI | RCATGY | 5 cut(s) | 538, 630, 890, 1099, 1263 |
| XmiI | GTMKAC | 2 cut(s) | 477, 1038 |
| XspI | CTAG | 6 cut(s) | 189, 566, 608, 767, 909, 926 |
| ZraI | GACGTC | 1 cut(s) | 249 |