Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| DNA_gyraseB | PF00204 | 248 - 360 | 6.7e-20 | DNA gyrase B |
| Toprim | PF01751 | 424 - 522 | 3.1e-07 | Toprim domain |
| TOPRIM_C | PF16898 | 538 - 665 | 2.6e-47 | C-terminal associated domain of TOPRIM |
| DNA_topoisoIV | PF00521 | 668 - 1127 | 2.8e-116 | DNA gyrase/topoisomerase IV, subunit A |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 2466, 2679 |
| AarI | CACCTGC | 1 cut(s) | 496 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 140 |
| Acc36I | ACCTGC | 2 cut(s) | 496, 3319 |
| AccB1I | GGYRCC | 1 cut(s) | 428 |
| AciI | CCGC | 7 cut(s) | 115, 207, 426, 474, 1719, 1991, 2497 |
| AclWI | GGATC | 9 cut(s) | 97, 234, 1527, 2001, 2525, 2538, 2720, 2733, 3384 |
| AcoI | YGGCCR | 2 cut(s) | 358, 3579 |
| AcuI | CTGAAG | 2 cut(s) | 59, 3195 |
| AcvI | CACGTG | 1 cut(s) | 1000 |
| AcyI | GRCGYC | 1 cut(s) | 429 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 1066, 1361, 2146, 3001 |
| AgeI | ACCGGT | 1 cut(s) | 407 |
| AhlI | ACTAGT | 1 cut(s) | 371 |
| AjnI | CCWGG | 4 cut(s) | 48, 1909, 2035, 2381 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 2714, 2746, 2969, 3001 |
| AleI | CACNNNNGTG | 1 cut(s) | 2142 |
| Alw21I | GWGCWC | 5 cut(s) | 60, 999, 2275, 2450, 3105 |
| Alw26I | GTCTC | 3 cut(s) | 160, 2469, 2621 |
| AlwI | GGATC | 9 cut(s) | 97, 234, 1527, 2001, 2525, 2538, 2720, 2733, 3384 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3209 |
| Ama87I | CYCGRG | 1 cut(s) | 579 |
| AoxI | GGCC | 6 cut(s) | 169, 358, 402, 1574, 2718, 3579 |
| ApeKI | GCWGC | 4 cut(s) | 423, 1450, 1546, 2810 |
| AseI | ATTAAT | 1 cut(s) | 2208 |
| AsiGI | ACCGGT | 1 cut(s) | 407 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 1164, 2925, 2940, 3116, 3627 |
| AspLEI | GCGC | 3 cut(s) | 431, 3478, 3671 |
| AspS9I | GGNCC | 8 cut(s) | 142, 231, 576, 721, 1336, 1575, 1725, 2667 |
| AsuC2I | CCSGG | 4 cut(s) | 168, 580, 581, 3411 |
| AsuHPI | GGTGA | 9 cut(s) | 211, 244, 406, 430, 595, 2156, 2522, 2936, 3061 |
| AvaI | CYCGRG | 1 cut(s) | 579 |
| AvaII | GGWCC | 7 cut(s) | 142, 231, 576, 721, 1336, 1725, 2667 |
| AxyI | CCTNAGG | 1 cut(s) | 2793 |
| BamHI | GGATCC | 2 cut(s) | 2530, 2725 |
| BanI | GGYRCC | 1 cut(s) | 428 |
| BanII | GRGCYC | 2 cut(s) | 2450, 3105 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 3632, 3664 |
| BauI | CACGAG | 1 cut(s) | 1964 |
| BbrPI | CACGTG | 1 cut(s) | 1000 |
| BbsI | GAAGAC | 4 cut(s) | 23, 115, 1157, 1196 |
| Bbv12I | GWGCWC | 5 cut(s) | 60, 999, 2275, 2450, 3105 |
| BbvI | GCAGC | 4 cut(s) | 410, 1462, 1533, 2822 |
| BceAI | ACGGC | 2 cut(s) | 436, 496 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 166, 200 |
| BciT130I | CCWGG | 4 cut(s) | 50, 1911, 2037, 2383 |
| BclI | TGATCA | 4 cut(s) | 1503, 1513, 2758, 2898 |
| BcnI | CCSGG | 4 cut(s) | 168, 580, 581, 3411 |
| BcoDI | GTCTC | 3 cut(s) | 160, 2469, 2621 |
| BcuI | ACTAGT | 1 cut(s) | 371 |
| BfaI | CTAG | 6 cut(s) | 372, 780, 1238, 1793, 2780, 3633 |
| BfmI | CTRYAG | 2 cut(s) | 1448, 2769 |
| BfoI | RGCGCY | 1 cut(s) | 432 |
| BfuAI | ACCTGC | 2 cut(s) | 496, 3319 |
| BglII | AGATCT | 1 cut(s) | 180 |
| BisI | GCNGC | 7 cut(s) | 207, 424, 427, 1451, 1547, 1720, 2811 |
| BlsI | GCNGC | 7 cut(s) | 208, 425, 428, 1452, 1548, 1721, 2812 |
| BmcAI | AGTACT | 4 cut(s) | 1736, 1759, 2164, 2767 |
| Bme1390I | CCNGG | 8 cut(s) | 50, 168, 580, 581, 1911, 2037, 2383, 3411 |
| Bme18I | GGWCC | 7 cut(s) | 142, 231, 576, 721, 1336, 1725, 2667 |
| BmeT110I | CYCGRG | 1 cut(s) | 579 |
| BmgT120I | GGNCC | 8 cut(s) | 142, 231, 576, 721, 1336, 1575, 1725, 2667 |
| BmrFI | CCNGG | 8 cut(s) | 50, 168, 580, 581, 1911, 2037, 2383, 3411 |
| BmrI | ACTGGG | 1 cut(s) | 2095 |
| BmsI | GCATC | 7 cut(s) | 285, 404, 668, 1219, 1504, 3373, 3616 |
| BmuI | ACTGGG | 1 cut(s) | 2095 |
| BpiI | GAAGAC | 4 cut(s) | 23, 115, 1157, 1196 |
| BpmI | CTGGAG | 4 cut(s) | 1244, 2464, 2711, 2901 |
| Bpu10I | CCTNAGC | 2 cut(s) | 993, 1296 |
| BpuEI | CTTGAG | 7 cut(s) | 230, 1687, 2951, 3017, 3119, 3284, 3422 |
| BpuMI | CCSGG | 4 cut(s) | 168, 580, 581, 3411 |
| Bsa29I | ATCGAT | 1 cut(s) | 92 |
| BsaAI | YACGTR | 1 cut(s) | 1000 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1233, 1518 |
| BsaHI | GRCGYC | 1 cut(s) | 429 |
| BsaJI | CCNNGG | 8 cut(s) | 166, 234, 579, 682, 2140, 2534, 2721, 3410 |
| BsaWI | WCCGGW | 2 cut(s) | 407, 3266 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1514, 1544, 3550, 3580 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 1066, 1361, 2146, 3001 |
| Bse118I | RCCGGY | 1 cut(s) | 407 |
| Bse21I | CCTNAGG | 1 cut(s) | 2793 |
| Bse3DI | GCAATG | 4 cut(s) | 885, 1314, 1564, 3552 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1233, 1518 |
| BseBI | CCWGG | 4 cut(s) | 50, 1911, 2037, 2383 |
| BseCI | ATCGAT | 1 cut(s) | 92 |
| BseDI | CCNNGG | 8 cut(s) | 166, 234, 579, 682, 2140, 2534, 2721, 3410 |
| BseGI | GGATG | 6 cut(s) | 419, 683, 807, 1234, 2332, 2454 |
| BseJI | GATNNNNATC | 2 cut(s) | 1233, 1518 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 1066, 1361, 2146, 3001 |
| BseMI | GCAATG | 4 cut(s) | 885, 1314, 1564, 3552 |
| BseMII | CTCAG | 7 cut(s) | 984, 1652, 1668, 2650, 2784, 3216, 3273 |
| BseRI | GAGGAG | 5 cut(s) | 541, 1892, 1982, 2810, 3419 |
| BseXI | GCAGC | 4 cut(s) | 410, 1462, 1533, 2822 |
| BseYI | CCCAGC | 1 cut(s) | 1313 |
| BsgI | GTGCAG | 1 cut(s) | 3588 |
| Bsh1285I | CGRYCG | 1 cut(s) | 24 |
| BshFI | GGCC | 6 cut(s) | 171, 360, 404, 1576, 2720, 3581 |
| BshNI | GGYRCC | 1 cut(s) | 428 |
| BshTI | ACCGGT | 1 cut(s) | 407 |
| BshVI | ATCGAT | 1 cut(s) | 92 |
| BsiEI | CGRYCG | 1 cut(s) | 24 |
| BsiHKAI | GWGCWC | 5 cut(s) | 60, 999, 2275, 2450, 3105 |
| BsiHKCI | CYCGRG | 1 cut(s) | 579 |
| BsiSI | CCGG | 7 cut(s) | 168, 344, 408, 580, 3267, 3410, 3582 |
| BslFI | GGGAC | 8 cut(s) | 149, 311, 707, 1265, 1349, 1790, 2251, 2449 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 1066, 1361, 2146, 3001 |
| BsmAI | GTCTC | 3 cut(s) | 160, 2469, 2621 |
| BsmBI | CGTCTC | 1 cut(s) | 160 |
| BsmFI | GGGAC | 8 cut(s) | 149, 311, 707, 1265, 1349, 1790, 2251, 2449 |
| BsmI | GAATGC | 2 cut(s) | 2125, 3478 |
| BsnI | GGCC | 6 cut(s) | 171, 360, 404, 1576, 2720, 3581 |
| BsoBI | CYCGRG | 1 cut(s) | 579 |
| Bsp1286I | GDGCHC | 5 cut(s) | 60, 999, 2275, 2450, 3105 |
| Bsp19I | CCATGG | 2 cut(s) | 234, 2140 |
| BspACI | CCGC | 7 cut(s) | 115, 207, 426, 474, 1719, 1991, 2497 |
| BspANI | GGCC | 6 cut(s) | 171, 360, 404, 1576, 2720, 3581 |
| BspCNI | CTCAG | 7 cut(s) | 985, 1651, 1669, 2649, 2785, 3215, 3274 |
| BspDI | ATCGAT | 1 cut(s) | 92 |
| BspHI | TCATGA | 1 cut(s) | 1506 |
| BspMAI | CTGCAG | 1 cut(s) | 1452 |
| BspMI | ACCTGC | 2 cut(s) | 496, 3319 |
| BspPI | GGATC | 9 cut(s) | 97, 234, 1527, 2001, 2525, 2538, 2720, 2733, 3384 |
| BspQI | GCTCTTC | 1 cut(s) | 38 |
| BspT107I | GGYRCC | 1 cut(s) | 428 |
| BsrDI | GCAATG | 4 cut(s) | 885, 1314, 1564, 3552 |
| BsrFI | RCCGGY | 1 cut(s) | 407 |
| BssAI | RCCGGY | 1 cut(s) | 407 |
| BssECI | CCNNGG | 8 cut(s) | 166, 234, 579, 682, 2140, 2534, 2721, 3410 |
| BssNI | GRCGYC | 1 cut(s) | 429 |
| BssSI | CACGAG | 1 cut(s) | 1964 |
| BssT1I | CCWWGG | 5 cut(s) | 234, 682, 2140, 2534, 2721 |
| Bst2BI | CACGAG | 1 cut(s) | 1964 |
| Bst2UI | CCWGG | 4 cut(s) | 50, 1911, 2037, 2383 |
| Bst6I | CTCTTC | 4 cut(s) | 38, 998, 3074, 3189 |
| BstACI | GRCGYC | 1 cut(s) | 429 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 64, 423, 2096, 2156 |
| BstBAI | YACGTR | 1 cut(s) | 1000 |
| BstC8I | GCNNGC | 6 cut(s) | 485, 1320, 1390, 1717, 1842, 3567 |
| BstDSI | CCRYGG | 2 cut(s) | 234, 2140 |
| BstEII | GGTNACC | 1 cut(s) | 250 |
| BstF5I | GGATG | 6 cut(s) | 419, 683, 807, 1234, 2332, 2454 |
| BstH2I | RGCGCY | 1 cut(s) | 432 |
| BstHHI | GCGC | 3 cut(s) | 431, 3478, 3671 |
| BstMAI | GTCTC | 3 cut(s) | 160, 2469, 2621 |
| BstMCI | CGRYCG | 1 cut(s) | 24 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 64, 423, 1238, 1319, 1582, 1906, 2096, 2156, 3302 |
| BstNI | CCWGG | 4 cut(s) | 50, 1911, 2037, 2383 |
| BstNSI | RCATGY | 2 cut(s) | 2875, 3669 |
| BstPI | GGTNACC | 1 cut(s) | 250 |
| BstSCI | CCNGG | 8 cut(s) | 48, 166, 578, 579, 1909, 2035, 2381, 3409 |
| BstSFI | CTRYAG | 2 cut(s) | 1448, 2769 |
| BstV1I | GCAGC | 4 cut(s) | 410, 1462, 1533, 2822 |
| BstV2I | GAAGAC | 4 cut(s) | 23, 115, 1157, 1196 |
| BstX2I | RGATCY | 5 cut(s) | 180, 1993, 2530, 2725, 3376 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2108 |
| BstYI | RGATCY | 5 cut(s) | 180, 1993, 2530, 2725, 3376 |
| Bsu15I | ATCGAT | 1 cut(s) | 92 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2793 |
| BsuRI | GGCC | 6 cut(s) | 171, 360, 404, 1576, 2720, 3581 |
| BsuTUI | ATCGAT | 1 cut(s) | 92 |
| BtgI | CCRYGG | 2 cut(s) | 234, 2140 |
| BtgZI | GCGATG | 1 cut(s) | 131 |
| BtsCI | GGATG | 6 cut(s) | 419, 683, 807, 1234, 2332, 2454 |
| BtsIMutI | CAGTG | 4 cut(s) | 286, 329, 1957, 2402 |
| BveI | ACCTGC | 2 cut(s) | 496, 3319 |
| Cac8I | GCNNGC | 6 cut(s) | 485, 1320, 1390, 1717, 1842, 3567 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3209 |
| CciI | TCATGA | 1 cut(s) | 1506 |
| CfoI | GCGC | 3 cut(s) | 431, 3478, 3671 |
| Cfr10I | RCCGGY | 1 cut(s) | 407 |
| Cfr13I | GGNCC | 8 cut(s) | 142, 231, 576, 721, 1336, 1575, 1725, 2667 |
| Cfr9I | CCCGGG | 1 cut(s) | 579 |
| ClaI | ATCGAT | 1 cut(s) | 92 |
| CsiI | ACCWGGT | 1 cut(s) | 2381 |
| CspAI | ACCGGT | 1 cut(s) | 407 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 1107, 1142, 3129, 3164 |
| DinI | GGCGCC | 1 cut(s) | 430 |
| DraI | TTTAAA | 1 cut(s) | 1615 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 140 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 140 |
| EaeI | YGGCCR | 2 cut(s) | 358, 3579 |
| Eam1104I | CTCTTC | 4 cut(s) | 38, 998, 3074, 3189 |
| EarI | CTCTTC | 4 cut(s) | 38, 998, 3074, 3189 |
| EciI | GGCGGA | 1 cut(s) | 2006 |
| Ecl136II | GAGCTC | 2 cut(s) | 2448, 3103 |
| Eco130I | CCWWGG | 5 cut(s) | 234, 682, 2140, 2534, 2721 |
| Eco24I | GRGCYC | 2 cut(s) | 2450, 3105 |
| Eco32I | GATATC | 1 cut(s) | 534 |
| Eco47I | GGWCC | 7 cut(s) | 142, 231, 576, 721, 1336, 1725, 2667 |
| Eco53kI | GAGCTC | 2 cut(s) | 2448, 3103 |
| Eco57I | CTGAAG | 2 cut(s) | 59, 3195 |
| Eco72I | CACGTG | 1 cut(s) | 1000 |
| Eco81I | CCTNAGG | 1 cut(s) | 2793 |
| Eco88I | CYCGRG | 1 cut(s) | 579 |
| Eco91I | GGTNACC | 1 cut(s) | 250 |
| EcoICRI | GAGCTC | 2 cut(s) | 2448, 3103 |
| EcoO109I | RGGNCCY | 1 cut(s) | 721 |
| EcoO65I | GGTNACC | 1 cut(s) | 250 |
| EcoRI | GAATTC | 2 cut(s) | 2936, 2957 |
| EcoRII | CCWGG | 4 cut(s) | 48, 1909, 2035, 2381 |
| EcoRV | GATATC | 1 cut(s) | 534 |
| EcoT14I | CCWWGG | 5 cut(s) | 234, 682, 2140, 2534, 2721 |
| EcoT22I | ATGCAT | 1 cut(s) | 3609 |
| EcoT38I | GRGCYC | 2 cut(s) | 2450, 3105 |
| EgeI | GGCGCC | 1 cut(s) | 430 |
| EheI | GGCGCC | 1 cut(s) | 430 |
| ErhI | CCWWGG | 5 cut(s) | 234, 682, 2140, 2534, 2721 |
| Esp3I | CGTCTC | 1 cut(s) | 160 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 1058, 1090, 1253, 1285, 1796, 1828, 2060, 2092 |
| FaqI | GGGAC | 8 cut(s) | 149, 311, 707, 1265, 1349, 1790, 2251, 2449 |
| FbaI | TGATCA | 4 cut(s) | 1503, 1513, 2758, 2898 |
| Fnu4HI | GCNGC | 7 cut(s) | 207, 424, 427, 1451, 1547, 1720, 2811 |
| FokI | GGATG | 6 cut(s) | 426, 690, 814, 1241, 2339, 2441 |
| FriOI | GRGCYC | 2 cut(s) | 2450, 3105 |
| Fsp4HI | GCNGC | 7 cut(s) | 207, 424, 427, 1451, 1547, 1720, 2811 |
| FspBI | CTAG | 6 cut(s) | 372, 780, 1238, 1793, 2780, 3633 |
| GlaI | GCGC | 3 cut(s) | 430, 3477, 3670 |
| GluI | GCNGC | 7 cut(s) | 207, 424, 427, 1451, 1547, 1720, 2811 |
| GsaI | CCCAGC | 1 cut(s) | 1317 |
| GsuI | CTGGAG | 4 cut(s) | 1244, 2464, 2711, 2901 |
| HaeII | RGCGCY | 1 cut(s) | 432 |
| HaeIII | GGCC | 6 cut(s) | 171, 360, 404, 1576, 2720, 3581 |
| HapII | CCGG | 7 cut(s) | 168, 344, 408, 580, 3267, 3410, 3582 |
| HhaI | GCGC | 3 cut(s) | 431, 3478, 3671 |
| Hin1I | GRCGYC | 1 cut(s) | 429 |
| Hin6I | GCGC | 3 cut(s) | 429, 3476, 3669 |
| HinP1I | GCGC | 3 cut(s) | 429, 3476, 3669 |
| HincII | GTYRAC | 1 cut(s) | 3264 |
| HindII | GTYRAC | 1 cut(s) | 3264 |
| HindIII | AAGCTT | 3 cut(s) | 1686, 1937, 3641 |
| HpaII | CCGG | 7 cut(s) | 168, 344, 408, 580, 3267, 3410, 3582 |
| HphI | GGTGA | 9 cut(s) | 211, 244, 406, 430, 595, 2156, 2522, 2936, 3061 |
| Hpy166II | GTNNAC | 7 cut(s) | 202, 1112, 1266, 1963, 2667, 2785, 3264 |
| Hpy8I | GTNNAC | 7 cut(s) | 202, 1112, 1266, 1963, 2667, 2785, 3264 |
| Hpy99I | CGWCG | 4 cut(s) | 137, 263, 389, 482 |
| HpyAV | CCTTC | 9 cut(s) | 691, 1242, 1763, 1924, 2022, 2225, 2420, 3367, 3481 |
| HpyCH4IV | ACGT | 5 cut(s) | 162, 261, 921, 999, 3501 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 64, 423, 1238, 1319, 1582, 1906, 2096, 2156, 3302 |
| HpySE526I | ACGT | 5 cut(s) | 162, 261, 921, 999, 3501 |
| Hsp92I | GRCGYC | 1 cut(s) | 429 |
| HspAI | GCGC | 3 cut(s) | 429, 3476, 3669 |
| KasI | GGCGCC | 1 cut(s) | 428 |
| KflI | GGGWCCC | 1 cut(s) | 721 |
| Ksp22I | TGATCA | 4 cut(s) | 1503, 1513, 2758, 2898 |
| LguI | GCTCTTC | 1 cut(s) | 38 |
| LmnI | GCTCC | 8 cut(s) | 55, 155, 1535, 1969, 2445, 3108, 3151, 3406 |
| Lsp1109I | GCAGC | 4 cut(s) | 410, 1462, 1533, 2822 |
| LweI | GCATC | 7 cut(s) | 285, 404, 668, 1219, 1504, 3373, 3616 |
| MabI | ACCWGGT | 1 cut(s) | 2381 |
| MaeI | CTAG | 6 cut(s) | 372, 780, 1238, 1793, 2780, 3633 |
| MaeII | ACGT | 5 cut(s) | 162, 261, 921, 999, 3501 |
| MaeIII | GTNAC | 7 cut(s) | 250, 377, 583, 848, 922, 2198, 2243 |
| MfeI | CAATTG | 1 cut(s) | 1845 |
| MflI | RGATCY | 5 cut(s) | 180, 1993, 2530, 2725, 3376 |
| MhlI | GDGCHC | 5 cut(s) | 60, 999, 2275, 2450, 3105 |
| Mly113I | GGCGCC | 1 cut(s) | 429 |
| MlyI | GAGTC | 6 cut(s) | 233, 1210, 1247, 1390, 2233, 2702 |
| MmeI | TCCRAC | 3 cut(s) | 2967, 2982, 3130 |
| Mph1103I | ATGCAT | 1 cut(s) | 3609 |
| MroXI | GAANNNNTTC | 5 cut(s) | 1164, 2925, 2940, 3116, 3627 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1344, 2142, 2174, 2268 |
| MspI | CCGG | 7 cut(s) | 168, 344, 408, 580, 3267, 3410, 3582 |
| MspR9I | CCNGG | 8 cut(s) | 50, 168, 580, 581, 1911, 2037, 2383, 3411 |
| MunI | CAATTG | 1 cut(s) | 1845 |
| Mva1269I | GAATGC | 2 cut(s) | 2125, 3478 |
| MvaI | CCWGG | 4 cut(s) | 50, 1911, 2037, 2383 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 64, 423, 1238, 1319, 1582, 1906, 2096, 2156, 3302 |
| NarI | GGCGCC | 1 cut(s) | 429 |
| NciI | CCSGG | 4 cut(s) | 168, 580, 581, 3411 |
| NcoI | CCATGG | 2 cut(s) | 234, 2140 |
| NmeAIII | GCCGAG | 1 cut(s) | 2269 |
| NmuCI | GTSAC | 4 cut(s) | 250, 583, 848, 922 |
| NsiI | ATGCAT | 1 cut(s) | 3609 |
| NspI | RCATGY | 2 cut(s) | 2875, 3669 |
| OliI | CACNNNNGTG | 1 cut(s) | 2142 |
| PagI | TCATGA | 1 cut(s) | 1506 |
| PaqCI | CACCTGC | 1 cut(s) | 496 |
| PciSI | GCTCTTC | 1 cut(s) | 38 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 132, 474 |
| PctI | GAATGC | 2 cut(s) | 2125, 3478 |
| PdmI | GAANNNNTTC | 5 cut(s) | 1164, 2925, 2940, 3116, 3627 |
| PinAI | ACCGGT | 1 cut(s) | 407 |
| PkrI | GCNGC | 7 cut(s) | 208, 425, 428, 1452, 1548, 1721, 2812 |
| Ple19I | CGATCG | 1 cut(s) | 24 |
| PleI | GAGTC | 6 cut(s) | 233, 1209, 1247, 1390, 2233, 2701 |
| PluTI | GGCGCC | 1 cut(s) | 432 |
| PmaCI | CACGTG | 1 cut(s) | 1000 |
| PmlI | CACGTG | 1 cut(s) | 1000 |
| PpsI | GAGTC | 6 cut(s) | 233, 1209, 1247, 1390, 2233, 2701 |
| Ppu21I | YACGTR | 1 cut(s) | 1000 |
| PpuMI | RGGWCCY | 1 cut(s) | 721 |
| PshBI | ATTAAT | 1 cut(s) | 2208 |
| PsiI | TTATAA | 2 cut(s) | 2466, 2679 |
| Psp124BI | GAGCTC | 2 cut(s) | 2450, 3105 |
| Psp5II | RGGWCCY | 1 cut(s) | 721 |
| Psp6I | CCWGG | 4 cut(s) | 48, 1909, 2035, 2381 |
| PspCI | CACGTG | 1 cut(s) | 1000 |
| PspEI | GGTNACC | 1 cut(s) | 250 |
| PspFI | CCCAGC | 1 cut(s) | 1313 |
| PspGI | CCWGG | 4 cut(s) | 48, 1909, 2035, 2381 |
| PspPI | GGNCC | 8 cut(s) | 142, 231, 576, 721, 1336, 1575, 1725, 2667 |
| PspPPI | RGGWCCY | 1 cut(s) | 721 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 3262, 3294 |
| PstI | CTGCAG | 1 cut(s) | 1452 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3209 |
| PsuI | RGATCY | 5 cut(s) | 180, 1993, 2530, 2725, 3376 |
| PvuI | CGATCG | 1 cut(s) | 24 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1344, 2142, 2174, 2268 |
| SacI | GAGCTC | 2 cut(s) | 2450, 3105 |
| SapI | GCTCTTC | 1 cut(s) | 38 |
| SatI | GCNGC | 7 cut(s) | 207, 424, 427, 1451, 1547, 1720, 2811 |
| Sau96I | GGNCC | 8 cut(s) | 142, 231, 576, 721, 1336, 1575, 1725, 2667 |
| ScaI | AGTACT | 4 cut(s) | 1736, 1759, 2164, 2767 |
| SchI | GAGTC | 6 cut(s) | 233, 1210, 1247, 1390, 2233, 2702 |
| ScrFI | CCNGG | 8 cut(s) | 50, 168, 580, 581, 1911, 2037, 2383, 3411 |
| SduI | GDGCHC | 5 cut(s) | 60, 999, 2275, 2450, 3105 |
| SexAI | ACCWGGT | 1 cut(s) | 2381 |
| SfaNI | GCATC | 7 cut(s) | 285, 404, 668, 1219, 1504, 3373, 3616 |
| SfcI | CTRYAG | 2 cut(s) | 1448, 2769 |
| SfoI | GGCGCC | 1 cut(s) | 430 |
| SgrAI | CRCCGGYG | 1 cut(s) | 407 |
| SinI | GGWCC | 7 cut(s) | 142, 231, 576, 721, 1336, 1725, 2667 |
| SmaI | CCCGGG | 1 cut(s) | 581 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1344, 2142, 2174, 2268 |
| SmlI | CTYRAG | 7 cut(s) | 245, 1702, 2930, 2996, 3098, 3299, 3401 |
| SmoI | CTYRAG | 7 cut(s) | 245, 1702, 2930, 2996, 3098, 3299, 3401 |
| SpeI | ACTAGT | 1 cut(s) | 371 |
| SsiI | CCGC | 7 cut(s) | 115, 207, 426, 474, 1719, 1991, 2497 |
| SspDI | GGCGCC | 1 cut(s) | 428 |
| SspI | AATATT | 2 cut(s) | 1429, 2206 |
| SspMI | CTAG | 6 cut(s) | 372, 780, 1238, 1793, 2780, 3633 |
| SstI | GAGCTC | 2 cut(s) | 2450, 3105 |
| StyD4I | CCNGG | 8 cut(s) | 48, 166, 578, 579, 1909, 2035, 2381, 3409 |
| StyI | CCWWGG | 5 cut(s) | 234, 682, 2140, 2534, 2721 |
| TaiI | ACGT | 5 cut(s) | 165, 264, 924, 1002, 3504 |
| TaqII | GACCGA | 1 cut(s) | 219 |
| TatI | WGTACW | 5 cut(s) | 498, 1734, 1757, 2162, 2765 |
| TauI | GCSGC | 3 cut(s) | 209, 429, 1722 |
| TscAI | CASTG | 4 cut(s) | 286, 329, 1957, 2409 |
| TseFI | GTSAC | 4 cut(s) | 250, 583, 848, 922 |
| TseI | GCWGC | 4 cut(s) | 423, 1450, 1546, 2810 |
| Tsp45I | GTSAC | 4 cut(s) | 250, 583, 848, 922 |
| TspGWI | ACGGA | 4 cut(s) | 142, 235, 2648, 3124 |
| TspMI | CCCGGG | 1 cut(s) | 579 |
| TspRI | CASTG | 4 cut(s) | 286, 329, 1957, 2409 |
| VpaK11BI | GGWCC | 7 cut(s) | 142, 231, 576, 721, 1336, 1725, 2667 |
| VspI | ATTAAT | 1 cut(s) | 2208 |
| XbaI | TCTAGA | 2 cut(s) | 779, 1792 |
| XceI | RCATGY | 2 cut(s) | 2875, 3669 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 2532, 2661, 3363, 3544 |
| XmaI | CCCGGG | 1 cut(s) | 579 |
| XmnI | GAANNNNTTC | 5 cut(s) | 1164, 2925, 2940, 3116, 3627 |
| XspI | CTAG | 6 cut(s) | 372, 780, 1238, 1793, 2780, 3633 |
| ZrmI | AGTACT | 4 cut(s) | 1736, 1759, 2164, 2767 |
| Zsp2I | ATGCAT | 1 cut(s) | 3609 |