RchiOBHm_Chr1g0375001

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001532
Physical Location & Seq
Forward (+)
62596464 .. 62602179
5716 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59875

Sequence Viewer

Length: 3645 bp
ATGGTGGTGGTGGAGAAGACGATCGAGGAAATGTTGTACCTGCAGAAGGGCCAGGTGGAGCACATCTTGCACCGACCCGATACCTACATCGGATCGATGACGAGGCTCACGGAGACGCTGTGGGTCTTCGAGAACGACGAGATGGTCCATCGCTCCGTCTCCTACGTCCCCGGCCTTTGCAAGATCTTCGATGAGATTCTGGTGAACGCCGCCGACAGTAAACGGAGGGATCACTGCATGGACTCTCTCAAGGTCACCATCGACGTCGAGCAGAACTGCGTCAGTGTTTATAACAACGGCGCCGGGATTCCCGTCGAGATTCACGGGCCGGCGAAGGCGTATGTTCCGGAGGTGATCTTCGGCCACCTCTTGACCGGGAACGGCTACGGCGCAAAGCTCACCAATATTTTCTCCACGGAATTCATCGTTGAAGTCGCCGACGGCAAGCAACAGAAGAAGTACAAGCAGGTGTTCACAAACAACATGCGGAAGAAATCTGAACCAGTCATCACTAAATGCAAAGAGAGTGAGAACTGGACCCGGGTGACATATAAGCCCGACTTGACAAAATTTAACATGACACATCTGGAAGATGATGTGGTAGCATTGATGAAAAAGCGAGTGATTGACATAGCTGGATGCCTCGGAAAGACTTTGAAGGTTGAGCTGAATGGAAACAGGGTCCCTGTAAAGTCATTTCTTCGTTATGTTGATCTCTACTTGCAATCTGCCGAAAAATTTAAAGACACTCAACTTCCAAGGATGATTGCGAAACTCAATGATAGGTGGGAAGTTTGTGTCAGTTTCAGCGATGGGCAGTTTCAACAGGTCAGCTTTGTGAATAGCATTGCTACAATCAAGGGGGGAACTCATGTTGATTATGTCACAGACCAGATAATTGCTCATGTGATGAATGTTGTAAATGGGAAGAAAAAAAATACCAACCTCAAGGCTCACAATGTGAGGAGCTATCTTTGGGTTTTTGTCAATGCTCTTATTGACAACCCTGCCTTTGATTCTCAAACAAAGGAAACTTTGACTATTTGCCCAAGCAGTTTTGGGTCTAAATGTGAACTACCCCAAGCCTTGTTAAAGAAAGTTGCAAAGTCTGGGATTGTGGAAAGTCTTCTCTATTCGGCAAGTGATGGAACAAAAACGGAGAGTCTTCTTAGCATCAACAAGCTGGAGGATGCTAACCTAGCAGGAGGAAGGGACTCTGAAAAGTGTACCTTGATTTTGACACAAGGAGATTCAGCTAAGGCTCTTGCAATGGCTGGACTTGCTGTTGTTGGTCGGGATCATTATGGCGTTTTTCCATTGAGAGGTAAATTGCTCAATGTAAGAGAAGCCAGCTTGTTTCAACTCAGGGACAATGAAGAAATCAAGAACATTATGAAGATTCTTGGTCTGCAGCAAGACAAACAATATACCGACGCCAAATCTTTAAGATATGGGCATCTGATGATCATGACTGATCAGGATCTCGATGGCTCCCACATAAAAGGGTTGCTGATCAATTTCATTCATTTCTTCTGGCCCTCTCTGCTAAAAGATCCTTCATTTTTTGTTGAGTTTATAACGCCTATTTTTAAGGCAACTCAGAAGAATGGGAAAGAAGTTGCATTGTATTCAATGCCCGAGTATGAAGCTTGGAAGGAAAGCTTGAGGGGTGATGCAAGTGGCTGGACCATTAAATACTATAAGGGGTTGGGAACAAGTACTTCAAAGGAAGGTAGGGACTACTTTCAGAATCTAGATATGCACAAGAAAAACTACGTTTGGGTAGATGAACGTGATGGGGAGGCTATTGAGCTTGCTTTTAGTAGGAAGAAAATAGAAGATAGGAAGAATTGGCTGCGGCAGTTTGAGCCTGGTACTCATCTTGATCAGAAGGAGAAGCTTGTAACATACAGAGATTTTGTTGACAAGGAGCTTATACAGTTTTCCATGGCTGATCTGCAAAGGTCGATACCATCAATGGTTGATGGATTGAAGCCAGGTCAAAGAAAGATACTGTTCTGCTCTTTTAAGAGGAACTTTATCAAGGAAGCAAAAGTTGCCCAGTTTTCTGGTTATGTGTCTGAGCATTCTGCCTACCACCATGGTGAGGAAAGTCTTGCCAGTACTATCATTGGAATGGCACAAGAATATGTGGGCAGTAACAATGTTAATCTCCTTCAACCAAATGGTCAATTTGGGACTCGTAACTTGGGAGGCAAAGACCATGCAAGTGCTCGTTATATATATACTCGGCTTTCTCCTATTACAAGGTTTCTTTTTCCAAAGGATGATGATAAACTTCTGGACTGCTTGAATGACGATGGGCAATCTATTGAGCCAACCTGGTATGTGCCGGTTATCCCAATGGTTCTTGTGAATGGTAGTGAAGGAATTGGGACTGGTTGGAGCTGTTATGTCCCTAATTATAATCCAAGAGACATCATTGCAAACATTAGGCGGTTTCTTAATGGTGATGAAATGGTGCCAATGGATCCGTGGTATAGAGGCTTTACTGGATTTATTGAGAAAACTGTCAAGGAATCTGGTACAAGCTACACAGTATGTGGGACTATTGAGGAAGTCAATGAGACAACACTCAGGATTTCGGAGCTACCAATCCGTAGGTCGAATCAAGATTATAAGGAATTTTTGGAGTCTATCTGTCAAGGGAATGACAAGGCCAAGGATCCTTTCATCGAGGGTTTCACACAGCATAGTGATGACAGTACTGTAGAAATTATAGTCTACTTGCCTGAGGAGAACCTGATGGCAGCCAGAAAAGATGGTTTGCTGAAGAAATTTAGACTGACCTCTACAATAGGCACAACTAACATGCACCTGTTTGATCCAAAGGGTGTGATTAAGAAATATGACACTCCAGAGCAGATTCTTGAGGAATTCTTTCACCTAAGACTTGAATTCTATGAGAAAAGGAAGAAAGTATTGTTGGATAACCTTGGGTTGGAATTGTTGAAATTGGAAAACAAGCTTCGATTCATCAGGAATCTCGTATGTGGAGATATAGTTGTGAGTAATAGGAAGAGAGCTGATCTATTCCTTGAGCTCAAACAGAAAGGTTTCAATCCTTTTCCAAAGAAAACTAAGGGTGTGGAGCCTGAGGTTGCTGGAGCAACTGAAGATACAGAAGAAACAGAAGTGAACCCAGAGGCAGTCATTGGTTATGGAGTACGAATCAGTGATTATGAGTATCTGCTCTCGATGGCAACTGGAACGTTGACCATTGAGAGTATTGAAGCCTTATCCCTTGAGAGGGATAAGGTCAATGCGCAGGTTCTGGAATTGATGGGCTCAACATCAACCTCACTGTGGTTGAAGGATCTCAATGCTCTTGAAATGGAACTTGATGAGCTGGAGAGAAGTGAGGCTTTGGCAGATGAGGCAAAAAGAAAATCAAGAAACAACCAGAAAGCTCCTGAGGTGAAGAAACAGAGAGTTGCAGCAAAAGGTATGAGCCATGACCCAATTATCCTTTCTGATGACGATGCCGAAGAAGAAATGGTGGATCTGACTTCATCTGAAGATAAGGAAACTGCGCAGAAGGAAGCACGTTGCAGAAAGGCCACAAGAACTGAAGATGGAAGAAGAGTCAGACAGAGAATAGATTTTGTTTGTGGAATTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 map01524
Pfam Domains
Protein Families
DNA_gyraseB DNA_topoisoIV HATPase_c TOPRIM_C Toprim

Protein Analysis

1214

Amino Acids

137.87

Weight (kDa)

6.15

Isoelectric Point (pI)

36.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_gyraseB PF00204 235 - 347 2.4e-21 DNA gyrase B
Toprim PF01751 411 - 524 1e-06 Toprim domain
TOPRIM_C PF16898 525 - 652 1.2e-48 C-terminal associated domain of TOPRIM
DNA_topoisoIV PF00521 655 - 1114 1.6e-112 DNA gyrase/topoisomerase IV, subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 291, 1577, 2427, 2640
AarI CACCTGC 1 cut(s) 457
AasI GACNNNNNNGTC 1 cut(s) 143
AatII GACGTC 1 cut(s) 267
Acc16I TGCGCA 2 cut(s) 3288, 3555
Acc36I ACCTGC 3 cut(s) 48, 457, 3280
AccB1I GGYRCC 2 cut(s) 299, 2482
AccI GTMKAC 1 cut(s) 2745
AccIII TCCGGA 1 cut(s) 346
AciI CCGC 4 cut(s) 210, 487, 1858, 2458
AclI AACGTT 1 cut(s) 3233
AcoI YGGCCR 1 cut(s) 361
AcsI RAATTY 7 cut(s) 419, 569, 737, 2645, 2798, 2897, 2918
AcuI CTGAAG 4 cut(s) 2813, 3156, 3558, 3612
AcyI GRCGYC 3 cut(s) 264, 300, 1434
AdeI CACNNNGTG 1 cut(s) 961
AfaI GTAC 9 cut(s) 38, 461, 1228, 1720, 1876, 2125, 2548, 2728, 3189
AfiI CCNNNNNNNGG 7 cut(s) 46, 1322, 2107, 2962, 3270, 3271, 3327
AjnI CCWGG 4 cut(s) 51, 1870, 1996, 2342
AjuI GAANNNNNNNTTGG 6 cut(s) 2675, 2707, 2930, 2962, 3475, 3507
AleI CACNNNNGTG 1 cut(s) 2103
Alw21I GWGCWC 3 cut(s) 63, 2236, 3066
Alw26I GTCTC 4 cut(s) 107, 163, 2430, 2582
AlwNI CAGNNNCTG 1 cut(s) 3295
Ama87I CYCGRG 2 cut(s) 540, 1637
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 7 cut(s) 49, 172, 326, 361, 1535, 2679, 3579
ApeKI GCWGC 4 cut(s) 1411, 1855, 2771, 3458
ApoI RAATTY 7 cut(s) 419, 569, 737, 2645, 2798, 2897, 2918
Asp700I GAANNNNTTC 3 cut(s) 1125, 2901, 3077
AspLEI GCGC 4 cut(s) 302, 392, 3289, 3556
AspS9I GGNCC 7 cut(s) 49, 145, 326, 537, 682, 1536, 1686
AsuC2I CCSGG 5 cut(s) 171, 304, 376, 541, 542
AvaI CYCGRG 2 cut(s) 540, 1637
AvaII GGWCC 4 cut(s) 145, 537, 682, 1686
AxyI CCTNAGG 3 cut(s) 2754, 3117, 3435
BamHI GGATCC 2 cut(s) 2491, 2686
BanI GGYRCC 2 cut(s) 299, 2482
BanII GRGCYC 2 cut(s) 3066, 3311
BbsI GAAGAC 4 cut(s) 23, 118, 1118, 1157
Bbv12I GWGCWC 3 cut(s) 63, 2236, 3066
BbvI GCAGC 4 cut(s) 1423, 1842, 2783, 3470
BceAI ACGGC 4 cut(s) 313, 397, 403, 457
BcgI CGANNNNNNTGC 2 cut(s) 169, 203
BciT130I CCWGG 4 cut(s) 53, 1872, 1998, 2344
BclI TGATCA 4 cut(s) 1464, 1474, 1512, 1885
BcnI CCSGG 5 cut(s) 171, 304, 376, 541, 542
BcoDI GTCTC 4 cut(s) 107, 163, 2430, 2582
BfaI CTAG 2 cut(s) 1199, 1754
BfmI CTRYAG 3 cut(s) 41, 1409, 2730
BfoI RGCGCY 1 cut(s) 303
BfuAI ACCTGC 3 cut(s) 48, 457, 3280
BglII AGATCT 1 cut(s) 183
BisI GCNGC 6 cut(s) 210, 1412, 1856, 1859, 2772, 3459
BlsI GCNGC 6 cut(s) 211, 1413, 1857, 1860, 2773, 3460
BmcAI AGTACT 3 cut(s) 1720, 2125, 2728
Bme1390I CCNGG 9 cut(s) 53, 171, 304, 376, 541, 542, 1872, 1998, 2344
Bme18I GGWCC 4 cut(s) 145, 537, 682, 1686
BmeT110I CYCGRG 2 cut(s) 540, 1637
BmgT120I GGNCC 7 cut(s) 49, 145, 326, 537, 682, 1536, 1686
BmiI GGNNCC 9 cut(s) 301, 539, 683, 684, 1492, 2484, 2493, 2688, 3114
BmrFI CCNGG 9 cut(s) 53, 171, 304, 376, 541, 542, 1872, 1998, 2344
BmrI ACTGGG 1 cut(s) 2056
BmsI GCATC 6 cut(s) 629, 1180, 1182, 1465, 1663, 3493
BmuI ACTGGG 1 cut(s) 2056
BpiI GAAGAC 4 cut(s) 23, 118, 1118, 1157
BpmI CTGGAG 4 cut(s) 1205, 2862, 3147, 3392
Bpu10I CCTNAGC 1 cut(s) 1257
BpuEI CTTGAG 6 cut(s) 233, 932, 1684, 2912, 3080, 3287
BpuMI CCSGG 5 cut(s) 171, 304, 376, 541, 542
Bsa29I ATCGAT 1 cut(s) 95
BsaBI GATNNNNATC 1 cut(s) 1479
BsaHI GRCGYC 3 cut(s) 264, 300, 1434
BsaWI WCCGGW 1 cut(s) 346
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 3009, 3039
Bsc4I CCNNNNNNNGG 7 cut(s) 46, 1322, 2107, 2962, 3270, 3271, 3327
Bse118I RCCGGY 2 cut(s) 328, 2353
Bse1I ACTGG 7 cut(s) 503, 539, 2062, 2121, 2404, 2518, 3232
Bse21I CCTNAGG 3 cut(s) 2754, 3117, 3435
Bse3DI GCAATG 3 cut(s) 846, 1275, 2442
Bse8I GATNNNNATC 1 cut(s) 1479
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 4 cut(s) 53, 1872, 1998, 2344
BseCI ATCGAT 1 cut(s) 95
BseGI GGATG 4 cut(s) 644, 768, 1195, 2293
BseJI GATNNNNATC 1 cut(s) 1479
BseLI CCNNNNNNNGG 7 cut(s) 46, 1322, 2107, 2962, 3270, 3271, 3327
BseMI GCAATG 3 cut(s) 846, 1275, 2442
BseMII CTCAG 7 cut(s) 1378, 1613, 2073, 2611, 2745, 3108, 3426
BseNI ACTGG 7 cut(s) 503, 539, 2062, 2121, 2404, 2518, 3232
BseRI GAGGAG 2 cut(s) 979, 2771
BseXI GCAGC 4 cut(s) 1423, 1842, 2783, 3470
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 7 cut(s) 51, 174, 328, 363, 1537, 2681, 3581
BshNI GGYRCC 2 cut(s) 299, 2482
BshVI ATCGAT 1 cut(s) 95
BsiEI CGRYCG 1 cut(s) 24
BsiHKAI GWGCWC 3 cut(s) 63, 2236, 3066
BsiHKCI CYCGRG 2 cut(s) 540, 1637
BsiSI CCGG 7 cut(s) 171, 303, 329, 347, 375, 541, 2354
BslFI GGGAC 9 cut(s) 152, 668, 1226, 1382, 1751, 2212, 2402, 2410, 2581
BslI CCNNNNNNNGG 7 cut(s) 46, 1322, 2107, 2962, 3270, 3271, 3327
BsmAI GTCTC 4 cut(s) 107, 163, 2430, 2582
BsmBI CGTCTC 2 cut(s) 107, 163
BsmFI GGGAC 9 cut(s) 152, 668, 1226, 1382, 1751, 2212, 2402, 2410, 2581
BsmI GAATGC 1 cut(s) 2086
BsnI GGCC 7 cut(s) 51, 174, 328, 363, 1537, 2681, 3581
BsoBI CYCGRG 2 cut(s) 540, 1637
Bsp1286I GDGCHC 4 cut(s) 63, 2236, 3066, 3311
Bsp13I TCCGGA 1 cut(s) 346
Bsp19I CCATGG 2 cut(s) 1947, 2101
BspACI CCGC 4 cut(s) 210, 487, 1858, 2458
BspANI GGCC 7 cut(s) 51, 174, 328, 363, 1537, 2681, 3581
BspCNI CTCAG 7 cut(s) 1377, 1612, 2074, 2610, 2746, 3109, 3427
BspDI ATCGAT 1 cut(s) 95
BspEI TCCGGA 1 cut(s) 346
BspHI TCATGA 1 cut(s) 1467
BspLI GGNNCC 9 cut(s) 301, 539, 683, 684, 1492, 2484, 2493, 2688, 3114
BspMAI CTGCAG 2 cut(s) 45, 1413
BspMI ACCTGC 3 cut(s) 48, 457, 3280
BspT107I GGYRCC 2 cut(s) 299, 2482
BsrDI GCAATG 3 cut(s) 846, 1275, 2442
BsrFI RCCGGY 2 cut(s) 328, 2353
BsrI ACTGG 7 cut(s) 503, 539, 2062, 2121, 2404, 2518, 3232
BssAI RCCGGY 2 cut(s) 328, 2353
BssNI GRCGYC 3 cut(s) 264, 300, 1434
BssT1I CCWWGG 5 cut(s) 758, 1947, 2101, 2682, 2956
Bst2UI CCWGG 4 cut(s) 53, 1872, 1998, 2344
Bst4CI ACNGT 8 cut(s) 218, 1941, 2016, 2533, 2560, 2726, 2731, 3327
Bst6I CTCTTC 2 cut(s) 3035, 3598
BstACI GRCGYC 3 cut(s) 264, 300, 1434
BstAPI GCANNNNNTGC 2 cut(s) 67, 2057
BstC8I GCNNGC 4 cut(s) 330, 446, 1351, 1815
BstDSI CCRYGG 4 cut(s) 414, 1947, 2101, 2495
BstEII GGTNACC 1 cut(s) 253
BstENI CCTNNNNNAGG 1 cut(s) 44
BstF5I GGATG 4 cut(s) 644, 768, 1195, 2293
BstH2I RGCGCY 1 cut(s) 303
BstHHI GCGC 4 cut(s) 302, 392, 3289, 3556
BstMAI GTCTC 4 cut(s) 107, 163, 2430, 2582
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 7 cut(s) 67, 1199, 1280, 1543, 1867, 2057, 3398
BstNI CCWGG 4 cut(s) 53, 1872, 1998, 2344
BstNSI RCATGY 2 cut(s) 487, 2836
BstPI GGTNACC 1 cut(s) 253
BstSCI CCNGG 9 cut(s) 51, 169, 302, 374, 539, 540, 1870, 1996, 2342
BstSFI CTRYAG 3 cut(s) 41, 1409, 2730
BstV1I GCAGC 4 cut(s) 1423, 1842, 2783, 3470
BstV2I GAAGAC 4 cut(s) 23, 118, 1118, 1157
BstX2I RGATCY 7 cut(s) 183, 1480, 1552, 2491, 2686, 3337, 3523
BstXI CCANNNNNNTGG 1 cut(s) 2069
BstYI RGATCY 7 cut(s) 183, 1480, 1552, 2491, 2686, 3337, 3523
Bsu15I ATCGAT 1 cut(s) 95
Bsu36I CCTNAGG 3 cut(s) 2754, 3117, 3435
BsuRI GGCC 7 cut(s) 51, 174, 328, 363, 1537, 2681, 3581
BsuTUI ATCGAT 1 cut(s) 95
BtgI CCRYGG 4 cut(s) 414, 1947, 2101, 2495
BtgZI GCGATG 2 cut(s) 134, 825
BtsCI GGATG 4 cut(s) 644, 768, 1195, 2293
BtsI GCAGTG 1 cut(s) 232
BtsIMutI CAGTG 4 cut(s) 232, 289, 3202, 3323
BveI ACCTGC 3 cut(s) 48, 457, 3280
Cac8I GCNNGC 4 cut(s) 330, 446, 1351, 1815
CaiI CAGNNNCTG 1 cut(s) 3295
CciI TCATGA 1 cut(s) 1467
CfoI GCGC 4 cut(s) 302, 392, 3289, 3556
Cfr10I RCCGGY 2 cut(s) 328, 2353
Cfr13I GGNCC 7 cut(s) 49, 145, 326, 537, 682, 1536, 1686
Cfr9I CCCGGG 1 cut(s) 540
ClaI ATCGAT 1 cut(s) 95
CseI GACGC 3 cut(s) 124, 268, 1442
CsiI ACCWGGT 1 cut(s) 2342
Csp6I GTAC 9 cut(s) 37, 460, 1227, 1719, 1875, 2124, 2547, 2727, 3188
CviQI GTAC 9 cut(s) 37, 460, 1227, 1719, 1875, 2124, 2547, 2727, 3188
DinI GGCGCC 1 cut(s) 301
DraI TTTAAA 1 cut(s) 742
DraIII CACNNNGTG 1 cut(s) 961
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 361
Eam1104I CTCTTC 2 cut(s) 3035, 3598
EarI CTCTTC 2 cut(s) 3035, 3598
Ecl136II GAGCTC 1 cut(s) 3064
Eco130I CCWWGG 5 cut(s) 758, 1947, 2101, 2682, 2956
Eco24I GRGCYC 2 cut(s) 3066, 3311
Eco47I GGWCC 4 cut(s) 145, 537, 682, 1686
Eco53kI GAGCTC 1 cut(s) 3064
Eco57I CTGAAG 4 cut(s) 2813, 3156, 3558, 3612
Eco81I CCTNAGG 3 cut(s) 2754, 3117, 3435
Eco88I CYCGRG 2 cut(s) 540, 1637
Eco91I GGTNACC 1 cut(s) 253
EcoICRI GAGCTC 1 cut(s) 3064
EcoNI CCTNNNNNAGG 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 682
EcoO65I GGTNACC 1 cut(s) 253
EcoRI GAATTC 3 cut(s) 419, 2897, 2918
EcoRII CCWGG 4 cut(s) 51, 1870, 1996, 2342
EcoT14I CCWWGG 5 cut(s) 758, 1947, 2101, 2682, 2956
EcoT38I GRGCYC 2 cut(s) 3066, 3311
EgeI GGCGCC 1 cut(s) 301
EheI GGCGCC 1 cut(s) 301
ErhI CCWWGG 5 cut(s) 758, 1947, 2101, 2682, 2956
Esp3I CGTCTC 2 cut(s) 107, 163
FaqI GGGAC 9 cut(s) 152, 668, 1226, 1382, 1751, 2212, 2402, 2410, 2581
FbaI TGATCA 4 cut(s) 1464, 1474, 1512, 1885
FblI GTMKAC 1 cut(s) 2745
Fnu4HI GCNGC 6 cut(s) 210, 1412, 1856, 1859, 2772, 3459
FokI GGATG 4 cut(s) 651, 775, 1202, 2300
FriOI GRGCYC 2 cut(s) 3066, 3311
Fsp4HI GCNGC 6 cut(s) 210, 1412, 1856, 1859, 2772, 3459
FspBI CTAG 2 cut(s) 1199, 1754
FspI TGCGCA 2 cut(s) 3288, 3555
GlaI GCGC 4 cut(s) 301, 391, 3288, 3555
GluI GCNGC 6 cut(s) 210, 1412, 1856, 1859, 2772, 3459
GsuI CTGGAG 4 cut(s) 1205, 2862, 3147, 3392
HaeII RGCGCY 1 cut(s) 303
HaeIII GGCC 7 cut(s) 51, 174, 328, 363, 1537, 2681, 3581
HapII CCGG 7 cut(s) 171, 303, 329, 347, 375, 541, 2354
HgaI GACGC 3 cut(s) 124, 268, 1442
HhaI GCGC 4 cut(s) 302, 392, 3289, 3556
Hin1I GRCGYC 3 cut(s) 264, 300, 1434
Hin6I GCGC 4 cut(s) 300, 390, 3287, 3554
HinP1I GCGC 4 cut(s) 300, 390, 3287, 3554
HincII GTYRAC 2 cut(s) 1924, 3237
HindII GTYRAC 2 cut(s) 1924, 3237
HindIII AAGCTT 4 cut(s) 1647, 1660, 1898, 2987
HpaII CCGG 7 cut(s) 171, 303, 329, 347, 375, 541, 2354
Hpy166II GTNNAC 9 cut(s) 205, 221, 474, 1073, 1227, 1924, 2746, 3160, 3237
Hpy8I GTNNAC 9 cut(s) 205, 221, 474, 1073, 1227, 1924, 2746, 3160, 3237
Hpy99I CGWCG 6 cut(s) 140, 266, 269, 317, 443, 1436
HpyCH4III ACNGT 8 cut(s) 218, 1941, 2016, 2533, 2560, 2726, 2731, 3327
HpyCH4IV ACGT 6 cut(s) 165, 264, 1776, 1792, 3233, 3568
HpyF10VI GCNNNNNNNGC 7 cut(s) 67, 1199, 1280, 1543, 1867, 2057, 3398
HpySE526I ACGT 6 cut(s) 165, 264, 1776, 1792, 3233, 3568
Hsp92I GRCGYC 3 cut(s) 264, 300, 1434
HspAI GCGC 4 cut(s) 300, 390, 3287, 3554
KasI GGCGCC 1 cut(s) 299
KflI GGGWCCC 1 cut(s) 682
Kpn2I TCCGGA 1 cut(s) 346
KroI GCCGGC 1 cut(s) 328
KroNI GCCGGC 1 cut(s) 330
Ksp22I TGATCA 4 cut(s) 1464, 1474, 1512, 1885
Lsp1109I GCAGC 4 cut(s) 1423, 1842, 2783, 3470
LweI GCATC 6 cut(s) 629, 1180, 1182, 1465, 1663, 3493
MabI ACCWGGT 1 cut(s) 2342
MaeI CTAG 2 cut(s) 1199, 1754
MaeII ACGT 6 cut(s) 165, 264, 1776, 1792, 3233, 3568
MaeIII GTNAC 6 cut(s) 253, 544, 883, 1903, 2159, 2204
MflI RGATCY 7 cut(s) 183, 1480, 1552, 2491, 2686, 3337, 3523
MhlI GDGCHC 4 cut(s) 63, 2236, 3066, 3311
Mly113I GGCGCC 1 cut(s) 300
MlyI GAGTC 6 cut(s) 236, 1171, 1208, 2194, 2663, 3615
MmeI TCCRAC 3 cut(s) 2384, 2928, 2943
MroI TCCGGA 1 cut(s) 346
MroNI GCCGGC 1 cut(s) 328
MroXI GAANNNNTTC 3 cut(s) 1125, 2901, 3077
MslI CAYNNNNRTG 4 cut(s) 2103, 2135, 2229, 2718
MspI CCGG 7 cut(s) 171, 303, 329, 347, 375, 541, 2354
MspR9I CCNGG 9 cut(s) 53, 171, 304, 376, 541, 542, 1872, 1998, 2344
Mva1269I GAATGC 1 cut(s) 2086
MvaI CCWGG 4 cut(s) 53, 1872, 1998, 2344
MwoI GCNNNNNNNGC 7 cut(s) 67, 1199, 1280, 1543, 1867, 2057, 3398
NaeI GCCGGC 1 cut(s) 330
NarI GGCGCC 1 cut(s) 300
NciI CCSGG 5 cut(s) 171, 304, 376, 541, 542
NcoI CCATGG 2 cut(s) 1947, 2101
NgoMIV GCCGGC 1 cut(s) 328
NlaIV GGNNCC 9 cut(s) 301, 539, 683, 684, 1492, 2484, 2493, 2688, 3114
NmeAIII GCCGAG 1 cut(s) 2230
NmuCI GTSAC 3 cut(s) 253, 544, 883
NsbI TGCGCA 2 cut(s) 3288, 3555
NspI RCATGY 2 cut(s) 487, 2836
OliI CACNNNNGTG 1 cut(s) 2103
PagI TCATGA 1 cut(s) 1467
PaqCI CACCTGC 1 cut(s) 457
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PctI GAATGC 1 cut(s) 2086
PdiI GCCGGC 1 cut(s) 330
PdmI GAANNNNTTC 3 cut(s) 1125, 2901, 3077
PkrI GCNGC 6 cut(s) 211, 1413, 1857, 1860, 2773, 3460
Ple19I CGATCG 1 cut(s) 24
PleI GAGTC 6 cut(s) 236, 1170, 1208, 2194, 2662, 3614
PluTI GGCGCC 1 cut(s) 303
PpsI GAGTC 6 cut(s) 236, 1170, 1208, 2194, 2662, 3614
PpuMI RGGWCCY 1 cut(s) 682
PsiI TTATAA 4 cut(s) 291, 1577, 2427, 2640
Psp124BI GAGCTC 1 cut(s) 3066
Psp1406I AACGTT 1 cut(s) 3233
Psp5II RGGWCCY 1 cut(s) 682
Psp6I CCWGG 4 cut(s) 51, 1870, 1996, 2342
PspEI GGTNACC 1 cut(s) 253
PspGI CCWGG 4 cut(s) 51, 1870, 1996, 2342
PspN4I GGNNCC 9 cut(s) 301, 539, 683, 684, 1492, 2484, 2493, 2688, 3114
PspPI GGNCC 7 cut(s) 49, 145, 326, 537, 682, 1536, 1686
PspPPI RGGWCCY 1 cut(s) 682
PstI CTGCAG 2 cut(s) 45, 1413
PstNI CAGNNNCTG 1 cut(s) 3295
PsuI RGATCY 7 cut(s) 183, 1480, 1552, 2491, 2686, 3337, 3523
PvuI CGATCG 1 cut(s) 24
RsaI GTAC 9 cut(s) 38, 461, 1228, 1720, 1876, 2125, 2548, 2728, 3189
RsaNI GTAC 9 cut(s) 37, 460, 1227, 1719, 1875, 2124, 2547, 2727, 3188
RseI CAYNNNNRTG 4 cut(s) 2103, 2135, 2229, 2718
SacI GAGCTC 1 cut(s) 3066
SatI GCNGC 6 cut(s) 210, 1412, 1856, 1859, 2772, 3459
Sau96I GGNCC 7 cut(s) 49, 145, 326, 537, 682, 1536, 1686
ScaI AGTACT 3 cut(s) 1720, 2125, 2728
SchI GAGTC 6 cut(s) 236, 1171, 1208, 2194, 2663, 3615
ScrFI CCNGG 9 cut(s) 53, 171, 304, 376, 541, 542, 1872, 1998, 2344
SduI GDGCHC 4 cut(s) 63, 2236, 3066, 3311
SexAI ACCWGGT 1 cut(s) 2342
SfaNI GCATC 6 cut(s) 629, 1180, 1182, 1465, 1663, 3493
SfcI CTRYAG 3 cut(s) 41, 1409, 2730
SfoI GGCGCC 1 cut(s) 301
SinI GGWCC 4 cut(s) 145, 537, 682, 1686
SmaI CCCGGG 1 cut(s) 542
SmiMI CAYNNNNRTG 4 cut(s) 2103, 2135, 2229, 2718
SmlI CTYRAG 6 cut(s) 248, 947, 1663, 2891, 3059, 3266
SmoI CTYRAG 6 cut(s) 248, 947, 1663, 2891, 3059, 3266
SsiI CCGC 4 cut(s) 210, 487, 1858, 2458
SspDI GGCGCC 1 cut(s) 299
SspI AATATT 1 cut(s) 406
SspMI CTAG 2 cut(s) 1199, 1754
SstI GAGCTC 1 cut(s) 3066
StyD4I CCNGG 9 cut(s) 51, 169, 302, 374, 539, 540, 1870, 1996, 2342
StyI CCWWGG 5 cut(s) 758, 1947, 2101, 2682, 2956
TaaI ACNGT 8 cut(s) 218, 1941, 2016, 2533, 2560, 2726, 2731, 3327
TaiI ACGT 6 cut(s) 168, 267, 1779, 1795, 3236, 3571
TatI WGTACW 4 cut(s) 459, 1718, 2123, 2726
TauI GCSGC 2 cut(s) 212, 1861
TscAI CASTG 4 cut(s) 239, 289, 3202, 3330
TseFI GTSAC 3 cut(s) 253, 544, 883
TseI GCWGC 4 cut(s) 1411, 1855, 2771, 3458
Tsp45I GTSAC 3 cut(s) 253, 544, 883
TspGWI ACGGA 7 cut(s) 125, 145, 238, 431, 1172, 2484, 2609
TspMI CCCGGG 1 cut(s) 540
TspRI CASTG 4 cut(s) 239, 289, 3202, 3330
VpaK11BI GGWCC 4 cut(s) 145, 537, 682, 1686
XagI CCTNNNNNAGG 1 cut(s) 44
XapI RAATTY 7 cut(s) 419, 569, 737, 2645, 2798, 2897, 2918
XbaI TCTAGA 1 cut(s) 1753
XceI RCATGY 2 cut(s) 487, 2836
XcmI CCANNNNNNNNNTGG 1 cut(s) 2493
XmaI CCCGGG 1 cut(s) 540
XmiI GTMKAC 1 cut(s) 2745
XmnI GAANNNNTTC 3 cut(s) 1125, 2901, 3077
XspI CTAG 2 cut(s) 1199, 1754
ZraI GACGTC 1 cut(s) 265
ZrmI AGTACT 3 cut(s) 1720, 2125, 2728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.