pycom07g02420

Calcium-dependent channel, 7TM region, putative phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005105
Physical Location & Seq
Forward (+)
1960224 .. 1966249
6026 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2094 bp
ATGAACCCTGAAAGCCTGATAGCTTCAGCGGCCATAAACATAGGGTTGGCTTTTTTCATCCTCACCCTCTTCTCCGTCCTCAAGAAACAACCCTCAAATGCCCCTATTTACTATCCTCGCCGCCTCTCTGAATCCCACCGCCAGAGCCACCGCCACATTCCCGACTTCGATGATAATTACGCCTCGTCGTCCGTCGGTTTCCGCAGCTTCCTTCCTTCTGTTACATGGATTTCCCGCGCGTTTCGCGTCTCCGAGGATGAAATCCTGCGATCAAATGGCCTCGACGCACTTATCATCATTAGGCTCTTCAAACTCGGGCTTTGGGTACACTTTTCATGTCTATGGTTTATATCTCTCTATGGCGTATACCTATTGCACAAGGAATATAAGGAGATTTTGGCTAAAAGGATTCAGCAACTTCGGAACATTAGGCATCAGCCTAATCAGTTTACTGTTCTGGTTCGGGAAATTCCTTTCTGCAGCGAACACAAGTCTCATGGGTGTTGTGTTAATAAGTTCTTCTCTAAGCATTACCCGTATGTGTATCATTCTTATCAGATTTTGTATGATGGAAAAGAATTTAAGGAGTTGATGGCAAAATCCATTGAAAAAAAGATAGAAGACTTGAGAGAGCGGTCTGTGGTGAAGAAACATAATAAAACCCCTTTGCTCTTGAATCCATCCCATGAAGATTCAGAGAAAATATTAGTCTATGAGGAAAATCTTGAAGAACTTCATCACAAGATACACCAATTACAGTGTGAAGATATGCTCCACGAAAAGGTGATTATCAGTGATACTAGCCTTAATAACCCATCGTGCAAAATAGTTTGGGAACATGAGTTGCCGGTCGCTTTTGTTACATTTAAGTCTCGGTTGGGTGCAGCCCTAGTTGCCCACTCTCAGCAGCACTCCAATCCGCTTCTTTGGATCACTGACGACGCTCCAGAGCCCAGGGATGTATCATGGAGAAATTTGGCAATCCCAGTCAGAGTTTTACCACTCTGCAATTTTGGAGTTATTGTTGCAGCCTCCCTTCTTACAATTTTCTTTGCCATCCCAGTCACTGCCGTCCAAGGAATTGCCAAATTTGAGAAATTAAAGAAATGGTTTCCTCCTGCCATGGCTTTACAGTTGATACCAGGGTTAAGCTCTGTCATAACGGGATATCTTCCGAGTGCCGTTCTGAAATTGTTTATCTACATTGTTCCATTTGCGATGCTCGGATTGGCTAAACTATCAGGATGTATCTCAAAGAGCAGGGAGGAGATAAAAGCATGCAACATGGTTTTTTATTTCCTAGTTGGAAACGTCTTCTTTTGGAGCTTGTTATCTGGCTCTTTATTAGATGAAATTGGTGAATCTTTTACTCACCCAAAAGATTTCCCCAGTCGCCTTGCTAGAGCTGTCTCTGCTCAAGCAGATTTCTTTGTGACATATATTTTGACAGATGGGCTATCGGGGTTTTCGTTGGAGATCCTTCAACCTGGATTGCTTTGTTGGGATGCTATAAGGTCCTGTACATATGGTCGTGGAAAAGAGAAAACTCCTTATCTTTATTCGTTACCTTACTTCAGAATCATTCCTTTAGTCTCGCTGTCTGTGCTGATCGGTACAGTATATGCAGTTGTGGCACCACTTCTGCTCCCATTTTTGATTGGTTACTTCTTGCTCGGCTATGCTGTATTCATAAATCAGATTGACGATGTTTATGAGACTATTTATGAGACATGTGGACAGTATTGGCCTTACATCCATCATTATATTCTCGTTTCAATAATTCTCATGCAAATCACTATGATTGGTCTTTTCGGACTGAAATCAAAACCAGCTGCATCCATTTCCACAATTCCACTTCTGGTGCTGACGTTGATGTTTAATGCATACTGCAAGATGCGTTTTCTTCCCGCATTCCACCTTTTATCTATTCAGAATGCCAAGGAATTTGATGAATTAGATGAGAAGTGCAATCAGGTGGAGTTTAACTATGAGAATGCTAGCACTGCTTATTGCCCCCCTTGTCTACGTCCCTTGAATTGCATGGAACCAGGGTCAAGCCACTCACCGCCCTTAACTACTTCATCATCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHM7_cyt RSN1_7TM RSN1_TM

Protein Analysis

698

Amino Acids

79.39

Weight (kDa)

8.17

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 6 - 121 1.1e-15 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 149 - 325 2.2e-18 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 338 - 604 2.7e-73 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1633
AccBSI CCGCTC 1 cut(s) 634
AccI GTMKAC 2 cut(s) 366, 2023
AccII CGCG 3 cut(s) 237, 239, 246
AclWI GGATC 2 cut(s) 938, 1471
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 5 cut(s) 468, 578, 973, 1088, 1943
AcuI CTGAAG 2 cut(s) 9, 1558
AfaI GTAC 3 cut(s) 327, 1522, 1615
AfiI CCNNNNNNNGG 1 cut(s) 781
AflIII ACRYGT 1 cut(s) 1730
AgsI TTSAA 7 cut(s) 310, 608, 676, 728, 1484, 1776, 2035
AjnI CCWGG 4 cut(s) 953, 1141, 1486, 2047
AluBI AGCT 6 cut(s) 23, 207, 1152, 1326, 1406, 1832
AluI AGCT 6 cut(s) 23, 207, 1152, 1326, 1406, 1832
Alw26I GTCTC 7 cut(s) 253, 498, 876, 1414, 1597, 1709, 1721
AlwI GGATC 2 cut(s) 938, 1471
AlwNI CAGNNNCTG 1 cut(s) 1067
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 3 cut(s) 30, 277, 1745
ApeKI GCWGC 6 cut(s) 204, 480, 884, 907, 1028, 1832
ApoI RAATTY 5 cut(s) 468, 578, 973, 1088, 1943
ArsI GACNNNNNNTTYG 2 cut(s) 1426, 1458
Asp700I GAANNNNTTC 1 cut(s) 732
AspLEI GCGC 1 cut(s) 239
AspS9I GGNCC 1 cut(s) 1515
AsuHPI GGTGA 6 cut(s) 55, 655, 796, 1364, 1370, 2055
AsuNHI GCTAGC 1 cut(s) 1997
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 1 cut(s) 1515
BaeI ACNNNNGTAYC 2 cut(s) 1130, 1163
BanI GGYRCC 1 cut(s) 1633
BanII GRGCYC 1 cut(s) 954
BbsI GAAGAC 2 cut(s) 627, 1306
BbvI GCAGC 6 cut(s) 216, 492, 896, 919, 1040, 1819
BccI CCATC 7 cut(s) 563, 586, 688, 823, 1064, 1445, 1764
BceAI ACGGC 2 cut(s) 1055, 1166
BciT130I CCWGG 4 cut(s) 955, 1143, 1488, 2049
BcoDI GTCTC 7 cut(s) 253, 498, 876, 1414, 1597, 1709, 1721
BfaI CTAG 5 cut(s) 801, 890, 1301, 1401, 1998
BfmI CTRYAG 1 cut(s) 478
BisI GCNGC 8 cut(s) 30, 121, 205, 481, 885, 908, 1029, 1833
BlsI GCNGC 8 cut(s) 31, 122, 206, 482, 886, 909, 1030, 1834
Bme1390I CCNGG 4 cut(s) 955, 1143, 1488, 2049
Bme18I GGWCC 1 cut(s) 1515
BmeT110I CYCGRG 1 cut(s) 314
BmgT120I GGNCC 1 cut(s) 1515
BmiI GGNNCC 2 cut(s) 1635, 2046
BmrFI CCNGG 4 cut(s) 955, 1143, 1488, 2049
BmrI ACTGGG 3 cut(s) 980, 1055, 1383
BmsI GCATC 5 cut(s) 442, 1209, 1495, 1844, 1884
BmtI GCTAGC 1 cut(s) 2001
BmuI ACTGGG 3 cut(s) 980, 1055, 1383
BpiI GAAGAC 2 cut(s) 627, 1306
BpmI CTGGAG 1 cut(s) 930
BpuEI CTTGAG 3 cut(s) 65, 646, 1401
BsaJI CCNNGG 8 cut(s) 252, 953, 954, 1075, 1122, 1142, 1938, 2048
BsaXI ACNNNNNCTCC 4 cut(s) 1008, 1038, 1629, 1659
Bsc4I CCNNNNNNNGG 1 cut(s) 781
Bse118I RCCGGY 1 cut(s) 847
Bse1I ACTGG 3 cut(s) 986, 1061, 1389
BseBI CCWGG 4 cut(s) 955, 1143, 1488, 2049
BseDI CCNNGG 8 cut(s) 252, 953, 954, 1075, 1122, 1142, 1938, 2048
BseGI GGATG 9 cut(s) 57, 262, 680, 964, 1056, 1250, 1510, 1752, 1835
BseLI CCNNNNNNNGG 1 cut(s) 781
BseMII CTCAG 1 cut(s) 917
BseNI ACTGG 3 cut(s) 986, 1061, 1389
BseRI GAGGAG 1 cut(s) 1280
BseXI GCAGC 6 cut(s) 216, 492, 896, 919, 1040, 1819
BsgI GTGCAG 1 cut(s) 903
Bsh1236I CGCG 3 cut(s) 237, 239, 246
Bsh1285I CGRYCG 1 cut(s) 852
BshFI GGCC 3 cut(s) 32, 279, 1747
BshNI GGYRCC 1 cut(s) 1633
BsiEI CGRYCG 1 cut(s) 852
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 1 cut(s) 848
BslFI GGGAC 1 cut(s) 2013
BslI CCNNNNNNNGG 1 cut(s) 781
BsmAI GTCTC 7 cut(s) 253, 498, 876, 1414, 1597, 1709, 1721
BsmBI CGTCTC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 2013
BsmI GAATGC 3 cut(s) 1910, 1939, 1999
BsnI GGCC 3 cut(s) 32, 279, 1747
BsoBI CYCGRG 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 954
Bsp1407I TGTACA 1 cut(s) 1520
Bsp143I GATC 4 cut(s) 269, 930, 1476, 1608
Bsp19I CCATGG 1 cut(s) 1122
BspANI GGCC 3 cut(s) 32, 279, 1747
BspCNI CTCAG 1 cut(s) 916
BspFNI CGCG 3 cut(s) 237, 239, 246
BspLI GGNNCC 2 cut(s) 1635, 2046
BspMAI CTGCAG 1 cut(s) 482
BspOI GCTAGC 1 cut(s) 2001
BspPI GGATC 2 cut(s) 938, 1471
BspQI GCTCTTC 1 cut(s) 311
BspT107I GGYRCC 1 cut(s) 1633
BsrBI CCGCTC 1 cut(s) 634
BsrFI RCCGGY 1 cut(s) 847
BsrGI TGTACA 1 cut(s) 1520
BsrI ACTGG 3 cut(s) 986, 1061, 1389
BssAI RCCGGY 1 cut(s) 847
BssECI CCNNGG 8 cut(s) 252, 953, 954, 1075, 1122, 1142, 1938, 2048
BssMI GATC 4 cut(s) 269, 930, 1476, 1608
BssNAI GTATAC 1 cut(s) 367
BssT1I CCWWGG 3 cut(s) 1075, 1122, 1938
Bst1107I GTATAC 1 cut(s) 367
Bst2UI CCWGG 4 cut(s) 955, 1143, 1488, 2049
Bst4CI ACNGT 5 cut(s) 454, 759, 1134, 1618, 1740
Bst6I CTCTTC 2 cut(s) 74, 311
BstAUI TGTACA 1 cut(s) 1520
BstC8I GCNNGC 2 cut(s) 1279, 1999
BstDEI CTNAG 2 cut(s) 525, 903
BstDSI CCRYGG 1 cut(s) 1122
BstF5I GGATG 9 cut(s) 57, 262, 680, 964, 1056, 1250, 1510, 1752, 1835
BstFNI CGCG 3 cut(s) 237, 239, 246
BstHHI GCGC 1 cut(s) 239
BstKTI GATC 4 cut(s) 272, 933, 1479, 1611
BstMAI GTCTC 7 cut(s) 253, 498, 876, 1414, 1597, 1709, 1721
BstMBI GATC 4 cut(s) 269, 930, 1476, 1608
BstMCI CGRYCG 1 cut(s) 852
BstMWI GCNNNNNNNGC 7 cut(s) 29, 243, 893, 1412, 1603, 1631, 2003
BstNI CCWGG 4 cut(s) 955, 1143, 1488, 2049
BstNSI RCATGY 2 cut(s) 1281, 1734
BstSCI CCNGG 4 cut(s) 953, 1141, 1486, 2047
BstSFI CTRYAG 1 cut(s) 478
BstUI CGCG 3 cut(s) 237, 239, 246
BstV1I GCAGC 6 cut(s) 216, 492, 896, 919, 1040, 1819
BstV2I GAAGAC 2 cut(s) 627, 1306
BstX2I RGATCY 1 cut(s) 1476
BstYI RGATCY 1 cut(s) 1476
BstZ17I GTATAC 1 cut(s) 367
BsuRI GGCC 3 cut(s) 32, 279, 1747
BtgI CCRYGG 1 cut(s) 1122
BtgZI GCGATG 1 cut(s) 1232
BtsCI GGATG 9 cut(s) 57, 262, 680, 964, 1056, 1250, 1510, 1752, 1835
BtsI GCAGTG 2 cut(s) 1065, 2001
BtsIMutI CAGTG 5 cut(s) 764, 799, 933, 1065, 2001
Cac8I GCNNGC 2 cut(s) 1279, 1999
CaiI CAGNNNCTG 1 cut(s) 1067
CfoI GCGC 1 cut(s) 239
Cfr10I RCCGGY 1 cut(s) 847
Cfr13I GGNCC 1 cut(s) 1515
CseI GACGC 3 cut(s) 235, 293, 950
Csp6I GTAC 3 cut(s) 326, 1521, 1614
CviQI GTAC 3 cut(s) 326, 1521, 1614
DdeI CTNAG 2 cut(s) 525, 903
DpnI GATC 4 cut(s) 271, 932, 1478, 1610
DpnII GATC 4 cut(s) 269, 930, 1476, 1608
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 2 cut(s) 74, 311
EarI CTCTTC 2 cut(s) 74, 311
Eco130I CCWWGG 3 cut(s) 1075, 1122, 1938
Eco24I GRGCYC 1 cut(s) 954
Eco32I GATATC 1 cut(s) 1169
Eco47I GGWCC 1 cut(s) 1515
Eco57I CTGAAG 2 cut(s) 9, 1558
Eco88I CYCGRG 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 1515
EcoRII CCWGG 4 cut(s) 953, 1141, 1486, 2047
EcoRV GATATC 1 cut(s) 1169
EcoT14I CCWWGG 3 cut(s) 1075, 1122, 1938
EcoT22I ATGCAT 1 cut(s) 1885
EcoT38I GRGCYC 1 cut(s) 954
ErhI CCWWGG 3 cut(s) 1075, 1122, 1938
Esp3I CGTCTC 1 cut(s) 253
FaqI GGGAC 1 cut(s) 2013
FauI CCCGC 2 cut(s) 242, 1915
FauNDI CATATG 1 cut(s) 1525
FblI GTMKAC 2 cut(s) 366, 2023
Fnu4HI GCNGC 8 cut(s) 30, 121, 205, 481, 885, 908, 1029, 1833
FokI GGATG 9 cut(s) 44, 269, 667, 971, 1043, 1257, 1517, 1739, 1822
FriOI GRGCYC 1 cut(s) 954
Fsp4HI GCNGC 8 cut(s) 30, 121, 205, 481, 885, 908, 1029, 1833
FspBI CTAG 5 cut(s) 801, 890, 1301, 1401, 1998
GlaI GCGC 1 cut(s) 238
GluI GCNGC 8 cut(s) 30, 121, 205, 481, 885, 908, 1029, 1833
GsuI CTGGAG 1 cut(s) 930
HaeIII GGCC 3 cut(s) 32, 279, 1747
HapII CCGG 1 cut(s) 848
HgaI GACGC 3 cut(s) 235, 293, 950
HhaI GCGC 1 cut(s) 239
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HinfI GANTC 6 cut(s) 131, 409, 676, 692, 1361, 1578
HpaII CCGG 1 cut(s) 848
HphI GGTGA 6 cut(s) 55, 655, 796, 1364, 1370, 2055
Hpy166II GTNNAC 5 cut(s) 328, 367, 450, 1736, 2024
Hpy188III TCNNGA 7 cut(s) 82, 161, 464, 673, 725, 947, 1242
Hpy8I GTNNAC 5 cut(s) 328, 367, 450, 1736, 2024
Hpy99I CGWCG 4 cut(s) 190, 197, 287, 944
HpyAV CCTTC 4 cut(s) 221, 225, 1046, 1490
HpyCH4III ACNGT 5 cut(s) 454, 759, 1134, 1618, 1740
HpyCH4IV ACGT 3 cut(s) 1311, 1868, 2026
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 243, 893, 1412, 1603, 1631, 2003
HpyF3I CTNAG 2 cut(s) 525, 903
HpySE526I ACGT 3 cut(s) 1311, 1868, 2026
HspAI GCGC 1 cut(s) 237
Kzo9I GATC 4 cut(s) 269, 930, 1476, 1608
LguI GCTCTTC 1 cut(s) 311
LmnI GCTCC 4 cut(s) 777, 949, 1323, 1650
Lsp1109I GCAGC 6 cut(s) 216, 492, 896, 919, 1040, 1819
LweI GCATC 5 cut(s) 442, 1209, 1495, 1844, 1884
MaeI CTAG 5 cut(s) 801, 890, 1301, 1401, 1998
MaeII ACGT 3 cut(s) 1311, 1868, 2026
MaeIII GTNAC 6 cut(s) 220, 859, 1063, 1432, 1563, 1661
MalI GATC 4 cut(s) 271, 932, 1478, 1610
MbiI CCGCTC 1 cut(s) 634
MboI GATC 4 cut(s) 269, 930, 1476, 1608
MflI RGATCY 1 cut(s) 1476
MhlI GDGCHC 1 cut(s) 954
MmeI TCCRAC 2 cut(s) 1285, 1452
Mph1103I ATGCAT 1 cut(s) 1885
MroXI GAANNNNTTC 1 cut(s) 732
MslI CAYNNNNRTG 1 cut(s) 340
MspA1I CMGCKG 2 cut(s) 29, 1832
MspI CCGG 1 cut(s) 848
MspR9I CCNGG 4 cut(s) 955, 1143, 1488, 2049
Mva1269I GAATGC 3 cut(s) 1910, 1939, 1999
MvaI CCWGG 4 cut(s) 955, 1143, 1488, 2049
MvnI CGCG 3 cut(s) 237, 239, 246
MwoI GCNNNNNNNGC 7 cut(s) 29, 243, 893, 1412, 1603, 1631, 2003
NcoI CCATGG 1 cut(s) 1122
NdeI CATATG 1 cut(s) 1525
NdeII GATC 4 cut(s) 269, 930, 1476, 1608
NheI GCTAGC 1 cut(s) 1997
NlaIV GGNNCC 2 cut(s) 1635, 2046
NmeAIII GCCGAG 1 cut(s) 1653
NmuCI GTSAC 2 cut(s) 1063, 1432
NsiI ATGCAT 1 cut(s) 1885
NspI RCATGY 2 cut(s) 1281, 1734
PaeI GCATGC 1 cut(s) 1281
PasI CCCWGGG 1 cut(s) 954
PciI ACATGT 1 cut(s) 1730
PciSI GCTCTTC 1 cut(s) 311
PcsI WCGNNNNNNNCGW 1 cut(s) 1466
PctI GAATGC 3 cut(s) 1910, 1939, 1999
PdmI GAANNNNTTC 1 cut(s) 732
PfeI GAWTC 6 cut(s) 131, 409, 676, 692, 1361, 1578
PkrI GCNGC 8 cut(s) 31, 122, 206, 482, 886, 909, 1030, 1834
PpuMI RGGWCCY 1 cut(s) 1515
PscI ACATGT 1 cut(s) 1730
Psp5II RGGWCCY 1 cut(s) 1515
Psp6I CCWGG 4 cut(s) 953, 1141, 1486, 2047
PspGI CCWGG 4 cut(s) 953, 1141, 1486, 2047
PspN4I GGNNCC 2 cut(s) 1635, 2046
PspPI GGNCC 1 cut(s) 1515
PspPPI RGGWCCY 1 cut(s) 1515
PstI CTGCAG 1 cut(s) 482
PstNI CAGNNNCTG 1 cut(s) 1067
PsuI RGATCY 1 cut(s) 1476
PvuII CAGCTG 1 cut(s) 1832
RsaI GTAC 3 cut(s) 327, 1522, 1615
RsaNI GTAC 3 cut(s) 326, 1521, 1614
RseI CAYNNNNRTG 1 cut(s) 340
SapI GCTCTTC 1 cut(s) 311
SatI GCNGC 8 cut(s) 30, 121, 205, 481, 885, 908, 1029, 1833
Sau3AI GATC 4 cut(s) 269, 930, 1476, 1608
Sau96I GGNCC 1 cut(s) 1515
ScrFI CCNGG 4 cut(s) 955, 1143, 1488, 2049
SduI GDGCHC 1 cut(s) 954
SfaNI GCATC 5 cut(s) 442, 1209, 1495, 1844, 1884
SfcI CTRYAG 1 cut(s) 478
SinI GGWCC 1 cut(s) 1515
SmiMI CAYNNNNRTG 1 cut(s) 340
SmlI CTYRAG 3 cut(s) 80, 625, 1416
SmoI CTYRAG 3 cut(s) 80, 625, 1416
SphI GCATGC 1 cut(s) 1281
SspI AATATT 1 cut(s) 705
SspMI CTAG 5 cut(s) 801, 890, 1301, 1401, 1998
StyD4I CCNGG 4 cut(s) 953, 1141, 1486, 2047
StyI CCWWGG 3 cut(s) 1075, 1122, 1938
TaaI ACNGT 5 cut(s) 454, 759, 1134, 1618, 1740
TaiI ACGT 3 cut(s) 1314, 1871, 2029
TaqI TCGA 2 cut(s) 168, 282
TatI WGTACW 1 cut(s) 1520
TauI GCSGC 2 cut(s) 32, 123
TfiI GAWTC 6 cut(s) 131, 409, 676, 692, 1361, 1578
TscAI CASTG 5 cut(s) 764, 799, 940, 1072, 2008
TseFI GTSAC 2 cut(s) 1063, 1432
TseI GCWGC 6 cut(s) 204, 480, 884, 907, 1028, 1832
Tsp45I GTSAC 2 cut(s) 1063, 1432
TspGWI ACGGA 2 cut(s) 64, 181
TspRI CASTG 5 cut(s) 764, 799, 940, 1072, 2008
VpaK11BI GGWCC 1 cut(s) 1515
XapI RAATTY 5 cut(s) 468, 578, 973, 1088, 1943
XceI RCATGY 2 cut(s) 1281, 1734
XmiI GTMKAC 2 cut(s) 366, 2023
XmnI GAANNNNTTC 1 cut(s) 732
XspI CTAG 5 cut(s) 801, 890, 1301, 1401, 1998
Zsp2I ATGCAT 1 cut(s) 1885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.