pycom07g16850

Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0018137
Physical Location & Seq
Forward (+)
19351289 .. 19351882
594 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g16850.1

Sequence Viewer

Length: 447 bp
ATGTTTAATAGCACAATTACTGAAGATAATTCTTTCTTAGGCCCCGCCGATGGGTCTATTATGATAACAGCAAGCCATTTCCCTTACAACAGGAATGGGTTCAAATTTTTCACAAACTCCGGAGGTCTTGGGAAACCTGACATTAAGAACATCTTAGAGCGTGCTGCAGATATATATGATAAATTTACAGTAGAAGGTTTGACAAACTCAAAACAAAAATTGCTTGCCTCCGTAAAGAGAGTTGATTACATGAATGTATATACATCTGATCTTGCAAAGGCAGTCCGCAAAGCTGCAAGAAATATAGGTATGACTTCCATGAAATCTTCAACGTGGTGGAAATGTACTGAGATACATCTTTTCATCCCTATCACGAAGTGCAATGTTGGTTGTAGTTCACTACTCTGTTCAGTGTCCAACACCAACACCAACTCCTTCATTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGM_PMM_I PGM_PMM_II PGM_PMM_III

Protein Analysis

149

Amino Acids

16.4

Weight (kDa)

9.25

Isoelectric Point (pI)

26.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGM_PMM_I PF02878 16 - 55 7.4e-08 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 119
AciI CCGC 2 cut(s) 45, 286
AcsI RAATTY 2 cut(s) 104, 182
AcuI CTGAAG 1 cut(s) 42
AdeI CACNNNGTG 1 cut(s) 378
AfaI GTAC 1 cut(s) 346
AfiI CCNNNNNNNGG 2 cut(s) 50, 51
AgsI TTSAA 2 cut(s) 103, 330
AluBI AGCT 1 cut(s) 293
AluI AGCT 1 cut(s) 293
Aor13HI TCCGGA 1 cut(s) 119
AoxI GGCC 1 cut(s) 40
ApeKI GCWGC 2 cut(s) 164, 293
ApoI RAATTY 2 cut(s) 104, 182
Asp700I GAANNNNTTC 1 cut(s) 98
AspS9I GGNCC 1 cut(s) 41
BbvI GCAGC 2 cut(s) 151, 280
BccI CCATC 1 cut(s) 44
BfmI CTRYAG 1 cut(s) 165
BisI GCNGC 2 cut(s) 165, 294
BlsI GCNGC 2 cut(s) 166, 295
BmgT120I GGNCC 1 cut(s) 41
BmiI GGNNCC 1 cut(s) 43
BsaWI WCCGGW 1 cut(s) 119
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 51
Bse3DI GCAATG 1 cut(s) 388
BseAI TCCGGA 1 cut(s) 119
BseGI GGATG 1 cut(s) 363
BseLI CCNNNNNNNGG 2 cut(s) 50, 51
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 1 cut(s) 339
BseXI GCAGC 2 cut(s) 151, 280
BshFI GGCC 1 cut(s) 42
BsiSI CCGG 1 cut(s) 120
BslI CCNNNNNNNGG 2 cut(s) 50, 51
BsnI GGCC 1 cut(s) 42
Bsp13I TCCGGA 1 cut(s) 119
Bsp143I GATC 1 cut(s) 268
BspACI CCGC 2 cut(s) 45, 286
BspANI GGCC 1 cut(s) 42
BspCNI CTCAG 1 cut(s) 340
BspEI TCCGGA 1 cut(s) 119
BspLI GGNNCC 1 cut(s) 43
BspMAI CTGCAG 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 388
BssMI GATC 1 cut(s) 268
Bst4CI ACNGT 1 cut(s) 190
BstC8I GCNNGC 3 cut(s) 73, 162, 225
BstDEI CTNAG 3 cut(s) 37, 154, 348
BstF5I GGATG 1 cut(s) 363
BstKTI GATC 1 cut(s) 271
BstMBI GATC 1 cut(s) 268
BstSFI CTRYAG 1 cut(s) 165
BstV1I GCAGC 2 cut(s) 151, 280
BsuRI GGCC 1 cut(s) 42
BtsCI GGATG 1 cut(s) 363
BtsIMutI CAGTG 1 cut(s) 417
Cac8I GCNNGC 3 cut(s) 73, 162, 225
Cfr13I GGNCC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 345
CviAII CATG 2 cut(s) 250, 319
CviJI RGCY 3 cut(s) 42, 75, 293
CviKI_1 RGCY 3 cut(s) 42, 75, 293
CviQI GTAC 1 cut(s) 345
DdeI CTNAG 3 cut(s) 37, 154, 348
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
DraIII CACNNNGTG 1 cut(s) 378
Eco57I CTGAAG 1 cut(s) 42
EcoO109I RGGNCCY 1 cut(s) 41
FaeI CATG 2 cut(s) 253, 322
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FatI CATG 2 cut(s) 249, 318
FauI CCCGC 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 165, 294
FokI GGATG 1 cut(s) 350
Fsp4HI GCNGC 2 cut(s) 165, 294
GluI GCNGC 2 cut(s) 165, 294
HaeIII GGCC 1 cut(s) 42
HapII CCGG 1 cut(s) 120
Hin1II CATG 2 cut(s) 253, 322
HpaII CCGG 1 cut(s) 120
Hpy166II GTNNAC 1 cut(s) 398
Hpy188I TCNGA 1 cut(s) 268
Hpy188III TCNNGA 2 cut(s) 120, 373
Hpy8I GTNNAC 1 cut(s) 398
HpyAV CCTTC 2 cut(s) 188, 445
HpyCH4III ACNGT 1 cut(s) 190
HpyCH4IV ACGT 1 cut(s) 332
HpyCH4V TGCA 4 cut(s) 167, 275, 296, 381
HpyF3I CTNAG 3 cut(s) 37, 154, 348
HpySE526I ACGT 1 cut(s) 332
Hsp92II CATG 2 cut(s) 253, 322
Kpn2I TCCGGA 1 cut(s) 119
Kzo9I GATC 1 cut(s) 268
LpnPI CCDG 3 cut(s) 76, 133, 150
Lsp1109I GCAGC 2 cut(s) 151, 280
MaeII ACGT 1 cut(s) 332
MalI GATC 1 cut(s) 270
MboI GATC 1 cut(s) 268
MboII GAAGA 2 cut(s) 35, 318
MluCI AATT 5 cut(s) 15, 28, 104, 182, 218
MmeI TCCRAC 1 cut(s) 441
MnlI CCTC 2 cut(s) 116, 238
MroI TCCGGA 1 cut(s) 119
MroXI GAANNNNTTC 1 cut(s) 98
MseI TTAA 2 cut(s) 6, 144
MspI CCGG 1 cut(s) 120
NdeII GATC 1 cut(s) 268
NlaIII CATG 2 cut(s) 253, 322
NlaIV GGNNCC 1 cut(s) 43
PdmI GAANNNNTTC 1 cut(s) 98
PkrI GCNGC 2 cut(s) 166, 295
PspN4I GGNNCC 1 cut(s) 43
PspPI GGNCC 1 cut(s) 41
PstI CTGCAG 1 cut(s) 169
RsaI GTAC 1 cut(s) 346
RsaNI GTAC 1 cut(s) 345
SaqAI TTAA 2 cut(s) 6, 144
SatI GCNGC 2 cut(s) 165, 294
Sau3AI GATC 1 cut(s) 268
Sau96I GGNCC 1 cut(s) 41
SetI ASST 6 cut(s) 127, 139, 199, 295, 310, 335
SfcI CTRYAG 1 cut(s) 165
Sse9I AATT 5 cut(s) 15, 28, 104, 182, 218
SsiI CCGC 2 cut(s) 45, 286
TaaI ACNGT 1 cut(s) 190
TaiI ACGT 1 cut(s) 335
TasI AATT 5 cut(s) 15, 28, 104, 182, 218
TatI WGTACW 1 cut(s) 344
Tru1I TTAA 2 cut(s) 6, 144
Tru9I TTAA 2 cut(s) 6, 144
TscAI CASTG 1 cut(s) 417
TseI GCWGC 2 cut(s) 164, 293
TspDTI ATGAA 4 cut(s) 266, 335, 352, 427
TspGWI ACGGA 1 cut(s) 220
TspRI CASTG 1 cut(s) 417
XapI RAATTY 2 cut(s) 104, 182
XmnI GAANNNNTTC 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.