pycom07g17020

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005758
Physical Location & Seq
Forward (+)
19553695 .. 19560591
6897 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2520 bp
ATGGCGGCGATAACGGGACCGGCATCGTCGTCGTTGATGATATCGTTGTCGTTTAAGCCGGCGTGCGTGCTGAGATCGACGAGGCCCGCCAAAACGACGCCGAAGATCAAGCGAGAGAGGCAGGGGCGGGCAGTGGTGGGGGACTTCGGACACTTGGGTCAGGTGGTTCGGAAGGACCTGGAGTTTCTGAAGACGGGGATTGGCAGAGGGATTGAGTGGGCGAATAAGGCTTTTCGAATTCCTGAGGTTTCCAAAGCGGTAGACGACGTCGTGTGGCTTCGCAACCTCGAAGACCCCGATGCGCCGCCATCACCAGCTCCTTCTTGGCCTCAGCCTGCCTATCCAGAGCTGTCCGGCGTGGATTTGCTCGTGGCTGATCTTAAAGCTTTGGAGACGTACGCTCTTTATTTCTATTATCTATCTAAGATTTGGTCTAAGCCACTTCCTGAAGTCTATGATCCAGAAAGTGTTGCTGAATATTTTAGCTGTAGGCCCCATGTAGTGGCCTTGAGACTTCTTGAGGTATTTTCCTCCTTTGCTTCTGCTGCAATCAGAATTCGGACCACAGGGATCAAAAAGTTCTTAAGGCTAAGTTCGGACGTGGATATTAATGAGAACATATCACAGTACAATTTCGGAATGGTGTTAAAAGAGACTATGCTAAGTCTGGGTCCCACTTTTATCAAAGTTGGTCAGTCCCTTTCCACCAGGCCGGACATTATTGGTGCTGAGATGGCCAAGCCATTGTCTGAGCTGCATGATCAAATTCCTCCATTTCCCAGGGCTATGGCTATGAAAATTATCAAAGAAGAATTAGGATCTCCTGCAGAGTCATTATTTAGCTACATTTCTGAGGAACCTGAGGCTGCTGCATCATTTGGTCAGGTCTACCGTGGGCGTACCCTTGATGGATTTGATGTTGCTATCAAAGTTCAACGGCCTAATTTGCATCACACTGTAGTGCGCGATATATATATTCTTCGCCTAGGGCTTGGGCTATTTCAAAACATAGCTAATAGAAAAAGTGACCTGCGTCTATACGCTGATGAGCTCGGGAAAGGTTTAGTTGGGGAATTGGATTATACTTTAGAGGCTGCAAATGCTTCCAAGTTTCAGGAAGCTCATTCCTCCTTTCCGTTCATGCTTGTGCCAAATGTTTATCAACATTTAAGTCGAAAGAGAGTTCTGACTATGGAGTGGATTGTTGGTGAGAGTCCAACTGATTTACTCTCTGTGTCTGCAGCTGGAAGCCCTGTTGAAAGTGGCTCTACATATTCAGAGAGGCAAATACTTGATGCCAAAAGACGTCTTCTTGATCTGGTTAAGAAAGGAGTGGAGGCATGCTTAGCTCAGCTCCTCGAAACAGGCTTATTGCATGCTGATCCACATCCTGGAAACTTGCGTTACACATCCTCGGGACAAATTGGGTTTTTGGATTTTGGCTTGCTTTGTCAGATGAAAAAGAAGCACCAATTTGCTATGCTTGCATCCATAGTGCACATAGTAAATGGAGATTGGGAATCCCTTGTTAATTCTTTGACTGAAATGGACGTTTCAAGGCCAGGGACTAATCTCAGGCGTGTGACTATGGATCTGGAATATGAACTGGGGGAAGTAGAATTTAGAGATGGAATTCCCGATGTCAAGTTCAGTAGGGTTCTGAGTAAAATCTGGTCCGTGGCCTTCAAGTATCATTTTCGCATGCCACCTTACTATTCGCTTGTTCTACGTTCTCTTGCTTCATTTGAAGGATTAGCTGTAGCTGCAGACAAAAATTTTAAGACATTTGAAGCTGCATACCCTTATGTTGTTCGGAAACTTCTCACTGAAAATTCAGCTGCGACAAGGAAAATATTACATTCGGTGGTTTTTAACAAGAAGAAGGAGTTCCAGTGGCAGAGGCTCTCTCTTTTCTTAAAAGTAGGTGCAACTAGGAAAGGTTTACATGAGATCATAGCACCTGAAGCTGATACTTCCGTCGGTTATTTACCATCAAGGGACGGTGGTGCAGTTGATGTTGCAAATTTTGTCTTGAGGATTTTACCATCCAAAGACGGTGTTGTGCTGAGAAGACTCTTGATGACTGCAGATGGAGCTTCACTGGTCCAAGCAATGGTATCCAAGGAGGCAAAGGTATACCGCCAGCAGTTTTGCAGGGTCATTGCAGATGTTCTATATCAATGGATGTATGCAGCTAATGGACGGGGCATTACAAAAACTCAGTATAGTTCCGATCTGAAAATGGCAGGCGGGCCTGAAAACAGAGAGAGAGGTCCATCTTCCAGAGCTCCCATTTATGATTATCGTGCCATCTACAGAGATCGGCGGCTCAGAGTGATTGTCTCGAATGTGCTAAACTCTGCAAGGAAAAATCCAATTCTAATGCTGAGGCTCTATTGGACTTCCTTCGTCATGTTTGCCACAGCGTTCGCTCTGGCTTGTCATCGTGCTTTATTATCCTTCGCCGAAGACCACTTAGGCCCGATATCTTTTGCTCCAAAGCAATATGCCATCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1 APH DCP2 NUDIX

Protein Analysis

840

Amino Acids

93.89

Weight (kDa)

9.32

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 252 - 511 3.3e-69 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 156
AatII GACGTC 2 cut(s) 270, 1309
Acc36I ACCTGC 1 cut(s) 1038
AccB7I CCANNNNNTGG 3 cut(s) 502, 1391, 2113
AccI GTMKAC 3 cut(s) 261, 888, 2136
AccII CGCG 1 cut(s) 966
AciI CCGC 8 cut(s) 5, 87, 127, 257, 305, 2140, 2250, 2326
AclWI GGATC 5 cut(s) 452, 578, 826, 1376, 1599
AcoI YGGCCR 1 cut(s) 735
AcsI RAATTY 8 cut(s) 237, 555, 765, 1619, 1632, 1774, 1831, 2023
AcuI CTGAAG 3 cut(s) 209, 468, 1983
AcyI GRCGYC 3 cut(s) 98, 267, 1306
AfaI GTAC 3 cut(s) 398, 629, 901
AfiI CCNNNNNNNGG 3 cut(s) 502, 1391, 2113
AflII CTTAAG 1 cut(s) 583
AgsI TTSAA 7 cut(s) 935, 1004, 1259, 1557, 1687, 1748, 1790
AjiI CACGTC 1 cut(s) 601
AjnI CCWGG 5 cut(s) 177, 707, 779, 1390, 1561
AleI CACNNNNGTG 1 cut(s) 959
Alw21I GWGCWC 3 cut(s) 1053, 1500, 2290
Alw26I GTCTC 4 cut(s) 386, 505, 647, 2347
Alw44I GTGCAC 1 cut(s) 1496
AlwI GGATC 5 cut(s) 452, 578, 826, 1376, 1599
Ama87I CYCGRG 2 cut(s) 1052, 1414
ApaLI GTGCAC 1 cut(s) 1496
ApoI RAATTY 8 cut(s) 237, 555, 765, 1619, 1632, 1774, 1831, 2023
AseI ATTAAT 1 cut(s) 609
Asp700I GAANNNNTTC 1 cut(s) 1886
AspA2I CCTAGG 1 cut(s) 985
AspLEI GCGC 2 cut(s) 304, 966
AsuHPI GGTGA 2 cut(s) 303, 1220
AsuII TTCGAA 1 cut(s) 235
AvaI CYCGRG 2 cut(s) 1052, 1414
AvaII GGWCC 7 cut(s) 17, 175, 561, 671, 1674, 2104, 2273
AvrII CCTAGG 1 cut(s) 985
AxyI CCTNAGG 2 cut(s) 243, 861
BaeGI GKGCMC 1 cut(s) 1500
BaeI ACNNNNGTAYC 2 cut(s) 1962, 1995
BalI TGGCCA 1 cut(s) 737
BanII GRGCYC 2 cut(s) 1053, 2290
BauI CACGAG 1 cut(s) 368
BbsI GAAGAC 5 cut(s) 197, 297, 1301, 2077, 2475
Bbv12I GWGCWC 3 cut(s) 1053, 1500, 2290
BbvCI CCTCAGC 2 cut(s) 330, 2387
BceAI ACGGC 1 cut(s) 953
BcgI CGANNNNNNTGC 2 cut(s) 12, 46
BciT130I CCWGG 5 cut(s) 179, 709, 781, 1392, 1563
BciVI GTATCC 1 cut(s) 2128
BclI TGATCA 1 cut(s) 760
BcoDI GTCTC 4 cut(s) 386, 505, 647, 2347
BfaI CTAG 2 cut(s) 986, 1932
BfmI CTRYAG 8 cut(s) 487, 825, 957, 1239, 1758, 1764, 2085, 2314
BfrI CTTAAG 1 cut(s) 583
BfuAI ACCTGC 1 cut(s) 1038
BfuI GTATCC 1 cut(s) 2128
BlnI CCTAGG 1 cut(s) 985
BlpI GCTNAGC 2 cut(s) 1345, 1350
Bme1390I CCNGG 5 cut(s) 179, 709, 781, 1392, 1563
Bme18I GGWCC 7 cut(s) 17, 175, 561, 671, 1674, 2104, 2273
BmeT110I CYCGRG 2 cut(s) 1052, 1414
BmgBI CACGTC 1 cut(s) 601
BmiI GGNNCC 5 cut(s) 18, 494, 672, 673, 858
BmrFI CCNGG 5 cut(s) 179, 709, 781, 1392, 1563
BmrI ACTGGG 1 cut(s) 1616
BmsI GCATC 6 cut(s) 32, 289, 881, 958, 1285, 1496
BmuI ACTGGG 1 cut(s) 1616
BoxI GACNNNNGTC 1 cut(s) 1032
BpiI GAAGAC 5 cut(s) 197, 297, 1301, 2077, 2475
BplI GAGNNNNNCTC 2 cut(s) 1891, 1923
BpmI CTGGAG 1 cut(s) 200
Bpu10I CCTNAGC 2 cut(s) 330, 2387
Bpu1102I GCTNAGC 2 cut(s) 1345, 1350
Bpu14I TTCGAA 1 cut(s) 235
BpuEI CTTGAG 3 cut(s) 529, 539, 2053
BsaBI GATNNNNATC 1 cut(s) 1386
BsaHI GRCGYC 3 cut(s) 98, 267, 1306
BsaJI CCNNGG 8 cut(s) 779, 780, 892, 985, 1413, 1562, 1677, 2121
BsaXI ACNNNNNCTCC 2 cut(s) 1877, 1907
Bsc4I CCNNNNNNNGG 3 cut(s) 502, 1391, 2113
Bse118I RCCGGY 2 cut(s) 19, 58
Bse1I ACTGG 4 cut(s) 1611, 1891, 2106, 2515
Bse21I CCTNAGG 2 cut(s) 243, 861
Bse3DI GCAATG 2 cut(s) 2118, 2160
Bse8I GATNNNNATC 1 cut(s) 1386
BseBI CCWGG 5 cut(s) 179, 709, 781, 1392, 1563
BseDI CCNNGG 8 cut(s) 779, 780, 892, 985, 1413, 1562, 1677, 2121
BseGI GGATG 6 cut(s) 1387, 1409, 1487, 2045, 2190, 2511
BseJI GATNNNNATC 1 cut(s) 1386
BseLI CCNNNNNNNGG 3 cut(s) 502, 1391, 2113
BseMI GCAATG 2 cut(s) 2118, 2160
BseNI ACTGG 4 cut(s) 1611, 1891, 2106, 2515
BseRI GAGGAG 1 cut(s) 1346
BseSI GKGCMC 1 cut(s) 1500
BsgI GTGCAG 1 cut(s) 2028
Bsh1236I CGCG 1 cut(s) 966
BsiHKAI GWGCWC 3 cut(s) 1053, 1500, 2290
BsiHKCI CYCGRG 2 cut(s) 1052, 1414
BsiSI CCGG 4 cut(s) 20, 59, 354, 713
BsiWI CGTACG 1 cut(s) 396
BslFI GGGAC 7 cut(s) 30, 155, 657, 682, 1431, 1579, 2012
BslI CCNNNNNNNGG 3 cut(s) 502, 1391, 2113
BsmAI GTCTC 4 cut(s) 386, 505, 647, 2347
BsmBI CGTCTC 1 cut(s) 386
BsmFI GGGAC 7 cut(s) 30, 155, 657, 682, 1431, 1579, 2012
BsoBI CYCGRG 2 cut(s) 1052, 1414
Bsp119I TTCGAA 1 cut(s) 235
Bsp1286I GDGCHC 3 cut(s) 1053, 1500, 2290
Bsp1720I GCTNAGC 2 cut(s) 1345, 1350
BspACI CCGC 8 cut(s) 5, 87, 127, 257, 305, 2140, 2250, 2326
BspFNI CGCG 1 cut(s) 966
BspLI GGNNCC 5 cut(s) 18, 494, 672, 673, 858
BspMAI CTGCAG 4 cut(s) 829, 1243, 1768, 2089
BspMI ACCTGC 1 cut(s) 1038
BspPI GGATC 5 cut(s) 452, 578, 826, 1376, 1599
BspT104I TTCGAA 1 cut(s) 235
BspTI CTTAAG 1 cut(s) 583
BsrDI GCAATG 2 cut(s) 2118, 2160
BsrFI RCCGGY 2 cut(s) 19, 58
BsrI ACTGG 4 cut(s) 1611, 1891, 2106, 2515
BssAI RCCGGY 2 cut(s) 19, 58
BssECI CCNNGG 8 cut(s) 779, 780, 892, 985, 1413, 1562, 1677, 2121
BssNAI GTATAC 1 cut(s) 2137
BssNI GRCGYC 3 cut(s) 98, 267, 1306
BssSI CACGAG 1 cut(s) 368
BssT1I CCWWGG 2 cut(s) 985, 2121
Bst1107I GTATAC 1 cut(s) 2137
Bst2BI CACGAG 1 cut(s) 368
Bst2UI CCWGG 5 cut(s) 179, 709, 781, 1392, 1563
Bst4CI ACNGT 5 cut(s) 627, 893, 958, 2003, 2057
BstACI GRCGYC 3 cut(s) 98, 267, 1306
BstAFI CTTAAG 1 cut(s) 583
BstBI TTCGAA 1 cut(s) 235
BstDSI CCRYGG 2 cut(s) 892, 1677
BstF5I GGATG 6 cut(s) 1387, 1409, 1487, 2045, 2190, 2511
BstFNI CGCG 1 cut(s) 966
BstHHI GCGC 2 cut(s) 304, 966
BstMAI GTCTC 4 cut(s) 386, 505, 647, 2347
BstNI CCWGG 5 cut(s) 179, 709, 781, 1392, 1563
BstNSI RCATGY 3 cut(s) 1344, 1379, 1705
BstPAI GACNNNNGTC 1 cut(s) 1032
BstSCI CCNGG 5 cut(s) 177, 707, 779, 1390, 1561
BstSFI CTRYAG 8 cut(s) 487, 825, 957, 1239, 1758, 1764, 2085, 2314
BstSLI GKGCMC 1 cut(s) 1500
BstUI CGCG 1 cut(s) 966
BstV2I GAAGAC 5 cut(s) 197, 297, 1301, 2077, 2475
BstX2I RGATCY 2 cut(s) 818, 1591
BstXI CCANNNNNNTGG 1 cut(s) 787
BstYI RGATCY 2 cut(s) 818, 1591
BstZ17I GTATAC 1 cut(s) 2137
Bsu36I CCTNAGG 2 cut(s) 243, 861
BsuI GTATCC 1 cut(s) 2128
BtgI CCRYGG 2 cut(s) 892, 1677
BtrI CACGTC 1 cut(s) 601
BtsCI GGATG 6 cut(s) 1387, 1409, 1487, 2045, 2190, 2511
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 5 cut(s) 138, 954, 1824, 1898, 2099
BveI ACCTGC 1 cut(s) 1038
CfoI GCGC 2 cut(s) 304, 966
Cfr10I RCCGGY 2 cut(s) 19, 58
CseI GACGC 2 cut(s) 106, 1022
Csp6I GTAC 3 cut(s) 397, 628, 900
CviAII CATG 8 cut(s) 497, 758, 1141, 1341, 1376, 1702, 1946, 2413
CviQI GTAC 3 cut(s) 397, 628, 900
DrdI GACNNNNNNGTC 1 cut(s) 156
DseDI GACNNNNNNGTC 1 cut(s) 156
EaeI YGGCCR 1 cut(s) 735
Ecl136II GAGCTC 2 cut(s) 1051, 2288
Eco130I CCWWGG 2 cut(s) 985, 2121
Eco24I GRGCYC 2 cut(s) 1053, 2290
Eco32I GATATC 2 cut(s) 42, 2487
Eco47I GGWCC 7 cut(s) 17, 175, 561, 671, 1674, 2104, 2273
Eco53kI GAGCTC 2 cut(s) 1051, 2288
Eco57I CTGAAG 3 cut(s) 209, 468, 1983
Eco81I CCTNAGG 2 cut(s) 243, 861
Eco88I CYCGRG 2 cut(s) 1052, 1414
EcoICRI GAGCTC 2 cut(s) 1051, 2288
EcoO109I RGGNCCY 3 cut(s) 175, 492, 671
EcoRI GAATTC 3 cut(s) 237, 555, 1632
EcoRII CCWGG 5 cut(s) 177, 707, 779, 1390, 1561
EcoRV GATATC 2 cut(s) 42, 2487
EcoT14I CCWWGG 2 cut(s) 985, 2121
EcoT38I GRGCYC 2 cut(s) 1053, 2290
ErhI CCWWGG 2 cut(s) 985, 2121
Esp3I CGTCTC 1 cut(s) 386
FaeI CATG 8 cut(s) 500, 761, 1144, 1344, 1379, 1705, 1949, 2416
FaqI GGGAC 7 cut(s) 30, 155, 657, 682, 1431, 1579, 2012
FatI CATG 8 cut(s) 496, 757, 1140, 1340, 1375, 1701, 1945, 2412
FauI CCCGC 3 cut(s) 94, 120, 2243
FbaI TGATCA 1 cut(s) 760
FblI GTMKAC 3 cut(s) 261, 888, 2136
FokI GGATG 6 cut(s) 1374, 1396, 1474, 2032, 2197, 2498
FriOI GRGCYC 2 cut(s) 1053, 2290
FspBI CTAG 2 cut(s) 986, 1932
GlaI GCGC 2 cut(s) 303, 965
GsuI CTGGAG 1 cut(s) 200
HapII CCGG 4 cut(s) 20, 59, 354, 713
HgaI GACGC 2 cut(s) 106, 1022
HhaI GCGC 2 cut(s) 304, 966
Hin1I GRCGYC 3 cut(s) 98, 267, 1306
Hin1II CATG 8 cut(s) 500, 761, 1144, 1344, 1379, 1705, 1949, 2416
Hin6I GCGC 2 cut(s) 302, 964
HinP1I GCGC 2 cut(s) 302, 964
HindIII AAGCTT 1 cut(s) 384
HinfI GANTC 4 cut(s) 830, 1213, 1520, 2073
HpaII CCGG 4 cut(s) 20, 59, 354, 713
HphI GGTGA 2 cut(s) 303, 1220
Hpy166II GTNNAC 5 cut(s) 262, 889, 1498, 1943, 2137
Hpy8I GTNNAC 5 cut(s) 262, 889, 1498, 1943, 2137
Hpy99I CGWCG 7 cut(s) 31, 34, 82, 100, 269, 272, 1982
HpyAV CCTTC 7 cut(s) 166, 330, 1693, 1742, 1876, 2416, 2470
HpyCH4III ACNGT 5 cut(s) 627, 893, 958, 2003, 2057
HpyCH4IV ACGT 6 cut(s) 267, 395, 600, 1306, 1551, 1729
HpySE526I ACGT 6 cut(s) 267, 395, 600, 1306, 1551, 1729
Hsp92I GRCGYC 3 cut(s) 98, 267, 1306
Hsp92II CATG 8 cut(s) 500, 761, 1144, 1344, 1379, 1705, 1949, 2416
HspAI GCGC 2 cut(s) 302, 964
KflI GGGWCCC 1 cut(s) 671
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
Ksp22I TGATCA 1 cut(s) 760
LmnI GCTCC 5 cut(s) 322, 1359, 2093, 2293, 2500
LweI GCATC 6 cut(s) 32, 289, 881, 958, 1285, 1496
MaeI CTAG 2 cut(s) 986, 1932
MaeII ACGT 6 cut(s) 267, 395, 600, 1306, 1551, 1729
MaeIII GTNAC 3 cut(s) 1025, 1403, 1582
MflI RGATCY 2 cut(s) 818, 1591
MhlI GDGCHC 3 cut(s) 1053, 1500, 2290
MlsI TGGCCA 1 cut(s) 737
MluNI TGGCCA 1 cut(s) 737
MlyI GAGTC 3 cut(s) 839, 1222, 2067
MmeI TCCRAC 1 cut(s) 1241
Mox20I TGGCCA 1 cut(s) 737
MroNI GCCGGC 1 cut(s) 58
MroXI GAANNNNTTC 1 cut(s) 1886
MscI TGGCCA 1 cut(s) 737
MslI CAYNNNNRTG 2 cut(s) 959, 1145
Msp20I TGGCCA 1 cut(s) 737
MspA1I CMGCKG 2 cut(s) 1244, 1838
MspCI CTTAAG 1 cut(s) 583
MspI CCGG 4 cut(s) 20, 59, 354, 713
MspR9I CCNGG 5 cut(s) 179, 709, 781, 1392, 1563
MvaI CCWGG 5 cut(s) 179, 709, 781, 1392, 1563
MvnI CGCG 1 cut(s) 966
NaeI GCCGGC 1 cut(s) 60
NgoMIV GCCGGC 1 cut(s) 58
NlaIII CATG 8 cut(s) 500, 761, 1144, 1344, 1379, 1705, 1949, 2416
NlaIV GGNNCC 5 cut(s) 18, 494, 672, 673, 858
NmuCI GTSAC 2 cut(s) 1025, 1582
NspI RCATGY 3 cut(s) 1344, 1379, 1705
NspV TTCGAA 1 cut(s) 235
OliI CACNNNNGTG 1 cut(s) 959
PaeI GCATGC 3 cut(s) 1344, 1379, 1705
PasI CCCWGGG 1 cut(s) 780
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PdiI GCCGGC 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 1886
PfeI GAWTC 1 cut(s) 1520
Pfl23II CGTACG 1 cut(s) 396
PflFI GACNNNGTC 1 cut(s) 266
PflMI CCANNNNNTGG 3 cut(s) 502, 1391, 2113
PfoI TCCNGGA 1 cut(s) 1390
PleI GAGTC 3 cut(s) 838, 1221, 2067
PpsI GAGTC 3 cut(s) 838, 1221, 2067
PpuMI RGGWCCY 2 cut(s) 175, 671
PshAI GACNNNNGTC 1 cut(s) 1032
PshBI ATTAAT 1 cut(s) 609
Psp124BI GAGCTC 2 cut(s) 1053, 2290
Psp5II RGGWCCY 2 cut(s) 175, 671
Psp6I CCWGG 5 cut(s) 177, 707, 779, 1390, 1561
PspGI CCWGG 5 cut(s) 177, 707, 779, 1390, 1561
PspLI CGTACG 1 cut(s) 396
PspN4I GGNNCC 5 cut(s) 18, 494, 672, 673, 858
PspPPI RGGWCCY 2 cut(s) 175, 671
PstI CTGCAG 4 cut(s) 829, 1243, 1768, 2089
PsuI RGATCY 2 cut(s) 818, 1591
PsyI GACNNNGTC 1 cut(s) 266
PvuII CAGCTG 2 cut(s) 1244, 1838
RsaI GTAC 3 cut(s) 398, 629, 901
RsaNI GTAC 3 cut(s) 397, 628, 900
RseI CAYNNNNRTG 2 cut(s) 959, 1145
SacI GAGCTC 2 cut(s) 1053, 2290
SchI GAGTC 3 cut(s) 839, 1222, 2067
ScrFI CCNGG 5 cut(s) 179, 709, 781, 1392, 1563
SduI GDGCHC 3 cut(s) 1053, 1500, 2290
SfaNI GCATC 6 cut(s) 32, 289, 881, 958, 1285, 1496
SfcI CTRYAG 8 cut(s) 487, 825, 957, 1239, 1758, 1764, 2085, 2314
SfuI TTCGAA 1 cut(s) 235
SinI GGWCC 7 cut(s) 17, 175, 561, 671, 1674, 2104, 2273
SmiMI CAYNNNNRTG 2 cut(s) 959, 1145
SmlI CTYRAG 4 cut(s) 508, 518, 583, 2032
SmoI CTYRAG 4 cut(s) 508, 518, 583, 2032
SphI GCATGC 3 cut(s) 1344, 1379, 1705
SsiI CCGC 8 cut(s) 5, 87, 127, 257, 305, 2140, 2250, 2326
SspI AATATT 2 cut(s) 479, 1854
SspMI CTAG 2 cut(s) 986, 1932
SstI GAGCTC 2 cut(s) 1053, 2290
StyD4I CCNGG 5 cut(s) 177, 707, 779, 1390, 1561
StyI CCWWGG 2 cut(s) 985, 2121
TaaI ACNGT 5 cut(s) 627, 893, 958, 2003, 2057
TaiI ACGT 6 cut(s) 270, 398, 603, 1309, 1554, 1732
TaqI TCGA 6 cut(s) 77, 235, 288, 1174, 1359, 2345
TatI WGTACW 1 cut(s) 627
TauI GCSGC 3 cut(s) 8, 307, 2329
TfiI GAWTC 1 cut(s) 1520
TscAI CASTG 5 cut(s) 138, 961, 1831, 1898, 2106
TseFI GTSAC 2 cut(s) 1025, 1582
Tsp45I GTSAC 2 cut(s) 1025, 1582
TspDTI ATGAA 5 cut(s) 809, 1129, 1472, 1617, 1731
TspGWI ACGGA 3 cut(s) 1125, 1666, 1966
TspRI CASTG 5 cut(s) 138, 961, 1831, 1898, 2106
Tth111I GACNNNGTC 1 cut(s) 266
Van91I CCANNNNNTGG 3 cut(s) 502, 1391, 2113
Vha464I CTTAAG 1 cut(s) 583
VneI GTGCAC 1 cut(s) 1496
VpaK11BI GGWCC 7 cut(s) 17, 175, 561, 671, 1674, 2104, 2273
VspI ATTAAT 1 cut(s) 609
XapI RAATTY 8 cut(s) 237, 555, 765, 1619, 1632, 1774, 1831, 2023
XceI RCATGY 3 cut(s) 1344, 1379, 1705
XcmI CCANNNNNNNNNTGG 1 cut(s) 321
XmaJI CCTAGG 1 cut(s) 985
XmiI GTMKAC 3 cut(s) 261, 888, 2136
XmnI GAANNNNTTC 1 cut(s) 1886
XspI CTAG 2 cut(s) 986, 1932
ZraI GACGTC 2 cut(s) 268, 1307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.