Rh1DG312000

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005758
Physical Location & Seq
Forward (+)
53887154 .. 53895222
8069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG312000.1

Sequence Viewer

Length: 2496 bp
ATGGCGACGGCGGCTGCGGCGTTGGTTCTCAAGCCGTCGTGCGTGCTGCGACGGCCGAGGGGAGCCAAAGCGACGACGTACAAGAGTAAGCGAGCAATAAAGGGACGAGCTTTGGGGGATCTCGGGCACTTCGGTCAGGTGGTCAGCAAGGATGTGGAGTTTCTGAAGCGGAGGATTGGTGGAGGATTTGAGTGGGCGAATAAGACTCTTCGAATTCCGGAGGTGTTCAAAGCCATCGACGACGTCGTTTGGCTTCGTAACCTCGAAGAACCCTATGCCCCTCCCTTGCCGGAGGCGCGTTGGCCTCAACCTTCCTATCCAGAATTCACTGGTGTCGATCTATTCATGGCGGATCTCAAAGCGTTAGAGGCATATGCTCTTTACTTTTATTATCTTTCTAAGAGTTGGTCCAAGCCGCTTCCTGAAGTCTATGATCCAGAAAGTGTTGCTGATTATTTCAGTTGCAGGCCTCACGTAGTCACCCTTAGACTTCTTGAGGTAGTTTCCTCCTTTGCTTTTGCTGCAATCAGAATTCGGACATCTGGGATCAAAAAGTATTTACGACCAACTTCGGGTGATGATTATGAAAGCTTATCACAATACAATTTTGGAATGGTGTTAAAAGAAACTATGCTAAATCTGGGTCCCACTTTTATCAAAGTTGGCCAGTCCCTTTCCACCAGACCAGACATTATTGGTGCTGAAATTTCCCAGCCATTGTCTGAGCTGCATGATCAAATCCCTCCTTTTTCCAGGGATGTGGCGATGAAAATTATCGAAGAAGAATTAGGATCTCCTGCTGAGTCACTCTATAGCTACATTTCTGAGAAACCTGAGGCAGCGGCATCTTTTGGTCAGGTCTACCGTGCACGTACTCATGATGGACTGGATGTTGCTGTTAAAGTTCAACGTCCTAACTTGCGTCATATTGTAGTGCGCGATATATACATTCTTCGCCTGGGGCTTGGACTATTGCAAAAGATAGCAAAGAGAAAAGGTGATCTCCGCCTATATGCCGATGAGCTTGGGAAAGGTTTTGTTGGAGAACTGGATTATACTTTAGAAGCTGCAAATGCTTCCAAGTTTCTGGAAGTTCATTCCTCCTATCCGTTCATGCGTGTGCCAAAAATATTCCAAAGTTTAAGTGGAAAGAGAGTTCTGACAATGGAGTGGATTGTTGGTGAGAGTCCAACCGACTTATTGTCTGTATCTTCTGGAAGCTCTACAGATGATAGCTCTACATATACAGAGAGGCAAAGATTAGATTCCAAAAGACGTCTTCTTGATCTGGTCAGGAAAGGGGTGGAGGCAGTACTAGTTCAGCTCCTCGAAACAGGATTATTGCATGCTGATCCACATCCTGGGAACCTACGTTACACATCTTCAGGACAAATAGGGTTTTTGGATTTTGGCCTGCTTTGTCAGATGGAAAAGAGGCATCAATATGCTATGCTTGCATCTATTGTCCACATTGTAAATGGAGATTGGGCATCCCTTGTCCAATGTTTGACTGAAATGGATGTTGTAAGACCAGGGACTAATATCAGACGTGTAACAATGGATCTGGAATATGACTTGGGGGAGGTAGAATTCAGAGATGGAGTTCCTGATGTGAAGTTCAGCAGGGTTTTGGGTAAGATCTGGTCCCTAGCCTTCAAGTATCATTTTCGCATGCCACCCTACTACTCGCTTGTTCTACGTTCTCTTGCTTCCTTTGAAGGATTAGCCGTCGCTGGAGATAGAAATTTTAAGACATTTGAAGCTGCATACCCTTATGTCGTTCGGAAACTACTTACTGAAAACTCAGCTGCCACAAGGAAAATTTTGCATTCGGTAGTTTTTAACAAAAAGAAGGAGTTCCAGTGGCAAAAGCTTGCTCTTTTCTTAAAAGTAGGCGCAACTAGGAAAGGTTTGAATGGGAGTATAGCATCAAAACTCGACACTTCACATGATTATTTACCTACAAGAGACAATGGTGAAATTGATGTTGCAAACTTGGTCCTGAAGCTTTTACCATCTAAAGATGGTGTTGTGCTGAGAAGACTCTTGATGACTGCAGATGGAGCTTCATTGATCCAAGCAATGGTATCCAAGGAAGCAAAGTTTTTCCGCCAGCAATTTTGCAGGTTTGTTGCAGACATTTTGCATCAATGGATGCTTGAAACGTTTGTGCGGGGCATTACAACAGTTCAGTATAGCTCTAATTTGAGAGTGGCAAGTGGACCTGACAACAAAGAACTAGGTCCATCTTCCAGACTATCTACTCCAATTTATGATTATCGCTCTGTCCTCAGAGATCGACGGCTCAAAGTGATTTTCTCAAACATAATAAACTCTGCAAGGAAAAATCCAGTACTGATGCTGAGGTTCTGTTGGACTTCCTTTGTTATGTCTGTTGTGGCTTTAGCTTTGGCTTCTCATCATGTTCTAATATCCTTCTCTGAAGCTTACTTGGGCCCGATATCTTTTGCTTCGAAACAGTATGCGATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1 APH DCP2 NUDIX

Protein Analysis

831

Amino Acids

93.53

Weight (kDa)

9.39

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 243 - 501 3.1e-68 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 246, 1279
Acc36I ACCTGC 1 cut(s) 2115
AccB7I CCANNNNNTGG 2 cut(s) 1361, 2083
AccI GTMKAC 1 cut(s) 861
AccII CGCG 2 cut(s) 298, 939
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 9 cut(s) 11, 17, 169, 350, 416, 842, 1006, 2110, 2173
AclI AACGTT 1 cut(s) 2165
AclWI GGATC 9 cut(s) 126, 360, 428, 554, 799, 1346, 1569, 2068, 2482
AcoI YGGCCR 2 cut(s) 53, 664
AcsI RAATTY 7 cut(s) 213, 323, 531, 705, 1589, 1744, 1821
AcuI CTGAAG 5 cut(s) 185, 444, 1368, 2024, 2463
AcyI GRCGYC 2 cut(s) 243, 1276
AfaI GTAC 4 cut(s) 80, 874, 1314, 2355
AfiI CCNNNNNNNGG 3 cut(s) 572, 1361, 2083
AflIII ACRYGT 1 cut(s) 1549
AgsI TTSAA 7 cut(s) 229, 908, 1657, 1718, 1760, 1915, 2162
AhdI GACNNNNNGTC 1 cut(s) 1201
AhlI ACTAGT 1 cut(s) 1315
AjiI CACGTC 1 cut(s) 1550
AjnI CCWGG 4 cut(s) 752, 957, 1360, 1531
AloI GAACNNNNNNTCC 2 cut(s) 1140, 1172
Alw21I GWGCWC 1 cut(s) 871
Alw26I GTCTC 1 cut(s) 1962
Alw44I GTGCAC 1 cut(s) 867
AlwI GGATC 9 cut(s) 126, 360, 428, 554, 799, 1346, 1569, 2068, 2482
Ama87I CYCGRG 1 cut(s) 122
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 6 cut(s) 53, 302, 467, 664, 1411, 2455
ApaI GGGCCC 1 cut(s) 2459
ApaLI GTGCAC 1 cut(s) 867
ApeKI GCWGC 8 cut(s) 14, 46, 521, 727, 839, 1067, 1763, 1808
ApoI RAATTY 7 cut(s) 213, 323, 531, 705, 1589, 1744, 1821
Asp700I GAANNNNTTC 1 cut(s) 1856
AspLEI GCGC 3 cut(s) 298, 939, 1898
AspS9I GGNCC 8 cut(s) 408, 644, 1644, 1999, 2222, 2243, 2455, 2456
AsuHPI GGTGA 5 cut(s) 472, 587, 1010, 1193, 1988
AsuII TTCGAA 2 cut(s) 211, 2474
AvaI CYCGRG 1 cut(s) 122
AvaII GGWCC 6 cut(s) 408, 644, 1644, 1999, 2222, 2243
AxyI CCTNAGG 1 cut(s) 834
BaeGI GKGCMC 3 cut(s) 129, 871, 2459
BalI TGGCCA 1 cut(s) 666
BanII GRGCYC 1 cut(s) 2459
BbsI GAAGAC 2 cut(s) 1271, 2047
Bbv12I GWGCWC 1 cut(s) 871
BbvCI CCTCAGC 1 cut(s) 2363
BbvI GCAGC 7 cut(s) 33, 508, 714, 851, 1054, 1750, 1795
BccI CCATC 8 cut(s) 242, 875, 1420, 1592, 2018, 2023, 2054, 2254
BceAI ACGGC 5 cut(s) 19, 24, 68, 1712, 2318
BciT130I CCWGG 4 cut(s) 754, 959, 1362, 1533
BciVI GTATCC 1 cut(s) 2098
BclI TGATCA 1 cut(s) 733
BcoDI GTCTC 1 cut(s) 1962
BcuI ACTAGT 1 cut(s) 1315
BfaI CTAG 4 cut(s) 1316, 1649, 1902, 2240
BfmI CTRYAG 3 cut(s) 811, 1224, 2055
BfuAI ACCTGC 1 cut(s) 2115
BfuI GTATCC 1 cut(s) 2098
BglII AGATCT 1 cut(s) 1638
BmcAI AGTACT 2 cut(s) 1314, 2355
Bme1390I CCNGG 4 cut(s) 754, 959, 1362, 1533
Bme18I GGWCC 6 cut(s) 408, 644, 1644, 1999, 2222, 2243
BmeRI GACNNNNNGTC 1 cut(s) 1201
BmeT110I CYCGRG 1 cut(s) 122
BmgBI CACGTC 1 cut(s) 1550
BmgT120I GGNCC 8 cut(s) 408, 644, 1644, 1999, 2222, 2243, 2455, 2456
BmiI GGNNCC 6 cut(s) 64, 645, 646, 1367, 1646, 2457
BmrFI CCNGG 4 cut(s) 754, 959, 1362, 1533
BmsI GCATC 8 cut(s) 854, 1447, 1466, 1499, 1937, 2145, 2155, 2349
BpiI GAAGAC 2 cut(s) 1271, 2047
BpmI CTGGAG 1 cut(s) 1755
Bpu10I CCTNAGC 1 cut(s) 2363
Bpu14I TTCGAA 2 cut(s) 211, 2474
BpuEI CTTGAG 2 cut(s) 14, 515
BsaAI YACGTR 2 cut(s) 475, 872
BsaBI GATNNNNATC 1 cut(s) 1356
BsaHI GRCGYC 2 cut(s) 243, 1276
BsaJI CCNNGG 6 cut(s) 56, 753, 958, 1361, 1532, 2091
BsaWI WCCGGW 1 cut(s) 217
BsaXI ACNNNNNCTCC 4 cut(s) 163, 193, 1847, 1877
Bsc4I CCNNNNNNNGG 3 cut(s) 572, 1361, 2083
Bse1I ACTGG 6 cut(s) 334, 667, 891, 1053, 1861, 2351
Bse21I CCTNAGG 1 cut(s) 834
Bse3DI GCAATG 1 cut(s) 2088
Bse8I GATNNNNATC 1 cut(s) 1356
BseAI TCCGGA 1 cut(s) 217
BseBI CCWGG 4 cut(s) 754, 959, 1362, 1533
BseDI CCNNGG 6 cut(s) 56, 753, 958, 1361, 1532, 2091
BseGI GGATG 7 cut(s) 157, 763, 895, 1357, 1490, 1525, 2160
BseJI GATNNNNATC 1 cut(s) 1356
BseLI CCNNNNNNNGG 3 cut(s) 572, 1361, 2083
BseMI GCAATG 1 cut(s) 2088
BseMII CTCAG 8 cut(s) 714, 792, 816, 825, 1818, 2027, 2305, 2354
BseNI ACTGG 6 cut(s) 334, 667, 891, 1053, 1861, 2351
BseRI GAGGAG 1 cut(s) 1316
BseSI GKGCMC 3 cut(s) 129, 871, 2459
BseX3I CGGCCG 1 cut(s) 53
BseXI GCAGC 7 cut(s) 33, 508, 714, 851, 1054, 1750, 1795
BseYI CCCAGC 1 cut(s) 711
Bsh1236I CGCG 2 cut(s) 298, 939
Bsh1285I CGRYCG 1 cut(s) 56
BshFI GGCC 6 cut(s) 55, 304, 469, 666, 1413, 2457
BsiEI CGRYCG 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 871
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 2 cut(s) 218, 290
BslFI GGGAC 5 cut(s) 117, 630, 655, 1549, 1630
BslI CCNNNNNNNGG 3 cut(s) 572, 1361, 2083
BsmAI GTCTC 1 cut(s) 1962
BsmFI GGGAC 5 cut(s) 117, 630, 655, 1549, 1630
BsmI GAATGC 1 cut(s) 1828
BsnI GGCC 6 cut(s) 55, 304, 469, 666, 1413, 2457
BsoBI CYCGRG 1 cut(s) 122
Bsp119I TTCGAA 2 cut(s) 211, 2474
Bsp120I GGGCCC 1 cut(s) 2455
Bsp1286I GDGCHC 3 cut(s) 129, 871, 2459
Bsp13I TCCGGA 1 cut(s) 217
BspACI CCGC 9 cut(s) 11, 17, 169, 350, 416, 842, 1006, 2110, 2173
BspANI GGCC 6 cut(s) 55, 304, 469, 666, 1413, 2457
BspCNI CTCAG 8 cut(s) 715, 793, 817, 826, 1817, 2028, 2304, 2355
BspEI TCCGGA 1 cut(s) 217
BspFNI CGCG 2 cut(s) 298, 939
BspHI TCATGA 1 cut(s) 877
BspLI GGNNCC 6 cut(s) 64, 645, 646, 1367, 1646, 2457
BspMAI CTGCAG 1 cut(s) 2059
BspMI ACCTGC 1 cut(s) 2115
BspPI GGATC 9 cut(s) 126, 360, 428, 554, 799, 1346, 1569, 2068, 2482
BspT104I TTCGAA 2 cut(s) 211, 2474
BsrDI GCAATG 1 cut(s) 2088
BsrI ACTGG 6 cut(s) 334, 667, 891, 1053, 1861, 2351
BssECI CCNNGG 6 cut(s) 56, 753, 958, 1361, 1532, 2091
BssNI GRCGYC 2 cut(s) 243, 1276
BssT1I CCWWGG 1 cut(s) 2091
Bst2UI CCWGG 4 cut(s) 754, 959, 1362, 1533
Bst4CI ACNGT 3 cut(s) 866, 2188, 2481
Bst6I CTCTTC 1 cut(s) 213
BstACI GRCGYC 2 cut(s) 243, 1276
BstBAI YACGTR 2 cut(s) 475, 872
BstBI TTCGAA 2 cut(s) 211, 2474
BstC8I GCNNGC 9 cut(s) 44, 93, 467, 1347, 1415, 1455, 1673, 1875, 2114
BstF5I GGATG 7 cut(s) 157, 763, 895, 1357, 1490, 1525, 2160
BstFNI CGCG 2 cut(s) 298, 939
BstHHI GCGC 3 cut(s) 298, 939, 1898
BstMAI GTCTC 1 cut(s) 1962
BstMCI CGRYCG 1 cut(s) 56
BstMWI GCNNNNNNNGC 9 cut(s) 11, 17, 52, 295, 368, 521, 1073, 1454, 2063
BstNI CCWGG 4 cut(s) 754, 959, 1362, 1533
BstNSI RCATGY 2 cut(s) 1349, 1675
BstSCI CCNGG 4 cut(s) 752, 957, 1360, 1531
BstSFI CTRYAG 3 cut(s) 811, 1224, 2055
BstSLI GKGCMC 3 cut(s) 129, 871, 2459
BstUI CGCG 2 cut(s) 298, 939
BstV1I GCAGC 7 cut(s) 33, 508, 714, 851, 1054, 1750, 1795
BstV2I GAAGAC 2 cut(s) 1271, 2047
BstX2I RGATCY 5 cut(s) 118, 352, 791, 1561, 1638
BstXI CCANNNNNNTGG 2 cut(s) 760, 1087
BstYI RGATCY 5 cut(s) 118, 352, 791, 1561, 1638
BstZI CGGCCG 1 cut(s) 53
Bsu36I CCTNAGG 1 cut(s) 834
BsuI GTATCC 1 cut(s) 2098
BsuRI GGCC 6 cut(s) 55, 304, 469, 666, 1413, 2457
BtgZI GCGATG 1 cut(s) 779
BtrI CACGTC 1 cut(s) 1550
BtsCI GGATG 7 cut(s) 157, 763, 895, 1357, 1490, 1525, 2160
BtsIMutI CAGTG 2 cut(s) 327, 1868
BveI ACCTGC 1 cut(s) 2115
Cac8I GCNNGC 9 cut(s) 44, 93, 467, 1347, 1415, 1455, 1673, 1875, 2114
CciI TCATGA 1 cut(s) 877
CfoI GCGC 3 cut(s) 298, 939, 1898
Cfr13I GGNCC 8 cut(s) 408, 644, 1644, 1999, 2222, 2243, 2455, 2456
CseI GACGC 1 cut(s) 911
Csp6I GTAC 4 cut(s) 79, 873, 1313, 2354
CviAII CATG 9 cut(s) 346, 731, 878, 1114, 1346, 1672, 1949, 2423, 2493
CviQI GTAC 4 cut(s) 79, 873, 1313, 2354
DriI GACNNNNNGTC 1 cut(s) 1201
EaeI YGGCCR 2 cut(s) 53, 664
EagI CGGCCG 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 213
Eam1105I GACNNNNNGTC 1 cut(s) 1201
EarI CTCTTC 1 cut(s) 213
EciI GGCGGA 3 cut(s) 365, 995, 2099
EclXI CGGCCG 1 cut(s) 53
Eco130I CCWWGG 1 cut(s) 2091
Eco147I AGGCCT 1 cut(s) 469
Eco24I GRGCYC 1 cut(s) 2459
Eco32I GATATC 1 cut(s) 2463
Eco47I GGWCC 6 cut(s) 408, 644, 1644, 1999, 2222, 2243
Eco52I CGGCCG 1 cut(s) 53
Eco57I CTGAAG 5 cut(s) 185, 444, 1368, 2024, 2463
Eco81I CCTNAGG 1 cut(s) 834
Eco88I CYCGRG 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 644
EcoRI GAATTC 4 cut(s) 213, 323, 531, 1589
EcoRII CCWGG 4 cut(s) 752, 957, 1360, 1531
EcoRV GATATC 1 cut(s) 2463
EcoT14I CCWWGG 1 cut(s) 2091
EcoT38I GRGCYC 1 cut(s) 2459
ErhI CCWWGG 1 cut(s) 2091
FaeI CATG 9 cut(s) 349, 734, 881, 1117, 1349, 1675, 1952, 2426, 2496
FaqI GGGAC 5 cut(s) 117, 630, 655, 1549, 1630
FatI CATG 9 cut(s) 345, 730, 877, 1113, 1345, 1671, 1948, 2422, 2492
FauI CCCGC 1 cut(s) 2166
FauNDI CATATG 1 cut(s) 373
FbaI TGATCA 1 cut(s) 733
FblI GTMKAC 1 cut(s) 861
FokI GGATG 7 cut(s) 164, 770, 902, 1344, 1477, 1532, 2167
FriOI GRGCYC 1 cut(s) 2459
FspBI CTAG 4 cut(s) 1316, 1649, 1902, 2240
GlaI GCGC 3 cut(s) 297, 938, 1897
GsaI CCCAGC 1 cut(s) 715
GsuI CTGGAG 1 cut(s) 1755
HaeIII GGCC 6 cut(s) 55, 304, 469, 666, 1413, 2457
HapII CCGG 2 cut(s) 218, 290
HgaI GACGC 1 cut(s) 911
HhaI GCGC 3 cut(s) 298, 939, 1898
Hin1I GRCGYC 2 cut(s) 243, 1276
Hin1II CATG 9 cut(s) 349, 734, 881, 1117, 1349, 1675, 1952, 2426, 2496
Hin6I GCGC 3 cut(s) 296, 937, 1896
HinP1I GCGC 3 cut(s) 296, 937, 1896
HindIII AAGCTT 4 cut(s) 589, 1871, 2006, 2445
HinfI GANTC 5 cut(s) 205, 803, 1186, 1265, 2043
HpaII CCGG 2 cut(s) 218, 290
HphI GGTGA 5 cut(s) 472, 587, 1010, 1193, 1988
Hpy166II GTNNAC 4 cut(s) 862, 869, 1468, 2222
Hpy8I GTNNAC 4 cut(s) 862, 869, 1468, 2222
HpyAV CCTTC 5 cut(s) 321, 1663, 1712, 1846, 2446
HpyCH4III ACNGT 3 cut(s) 866, 2188, 2481
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 17, 52, 295, 368, 521, 1073, 1454, 2063
Hsp92I GRCGYC 2 cut(s) 243, 1276
Hsp92II CATG 9 cut(s) 349, 734, 881, 1117, 1349, 1675, 1952, 2426, 2496
HspAI GCGC 3 cut(s) 296, 937, 1896
KflI GGGWCCC 1 cut(s) 644
Kpn2I TCCGGA 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 733
LmnI GCTCC 3 cut(s) 62, 1329, 2063
Lsp1109I GCAGC 7 cut(s) 33, 508, 714, 851, 1054, 1750, 1795
LweI GCATC 8 cut(s) 854, 1447, 1466, 1499, 1937, 2145, 2155, 2349
MaeI CTAG 4 cut(s) 1316, 1649, 1902, 2240
MaeIII GTNAC 5 cut(s) 257, 478, 804, 1373, 1552
MflI RGATCY 5 cut(s) 118, 352, 791, 1561, 1638
MhlI GDGCHC 3 cut(s) 129, 871, 2459
MlsI TGGCCA 1 cut(s) 666
MluNI TGGCCA 1 cut(s) 666
MlyI GAGTC 4 cut(s) 199, 812, 1195, 2037
MmeI TCCRAC 3 cut(s) 1021, 1214, 2354
Mox20I TGGCCA 1 cut(s) 666
MroI TCCGGA 1 cut(s) 217
MroXI GAANNNNTTC 1 cut(s) 1856
MscI TGGCCA 1 cut(s) 666
MseI TTAA 6 cut(s) 620, 900, 1142, 1749, 1842, 1886
MslI CAYNNNNRTG 2 cut(s) 1118, 1443
Msp20I TGGCCA 1 cut(s) 666
MspA1I CMGCKG 2 cut(s) 842, 1808
MspI CCGG 2 cut(s) 218, 290
MspR9I CCNGG 4 cut(s) 754, 959, 1362, 1533
Mva1269I GAATGC 1 cut(s) 1828
MvaI CCWGG 4 cut(s) 754, 959, 1362, 1533
MvnI CGCG 2 cut(s) 298, 939
MwoI GCNNNNNNNGC 9 cut(s) 11, 17, 52, 295, 368, 521, 1073, 1454, 2063
NdeI CATATG 1 cut(s) 373
NlaIII CATG 9 cut(s) 349, 734, 881, 1117, 1349, 1675, 1952, 2426, 2496
NlaIV GGNNCC 6 cut(s) 64, 645, 646, 1367, 1646, 2457
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 2 cut(s) 478, 804
NspI RCATGY 2 cut(s) 1349, 1675
NspV TTCGAA 2 cut(s) 211, 2474
PaeI GCATGC 2 cut(s) 1349, 1675
PagI TCATGA 1 cut(s) 877
PceI AGGCCT 1 cut(s) 469
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 1 cut(s) 1828
PdmI GAANNNNTTC 1 cut(s) 1856
PfeI GAWTC 1 cut(s) 1265
PflFI GACNNNGTC 1 cut(s) 242
PflMI CCANNNNNTGG 2 cut(s) 1361, 2083
PleI GAGTC 4 cut(s) 199, 811, 1194, 2037
PpsI GAGTC 4 cut(s) 199, 811, 1194, 2037
Ppu21I YACGTR 2 cut(s) 475, 872
PpuMI RGGWCCY 1 cut(s) 644
Psp1406I AACGTT 1 cut(s) 2165
Psp5II RGGWCCY 1 cut(s) 644
Psp6I CCWGG 4 cut(s) 752, 957, 1360, 1531
PspFI CCCAGC 1 cut(s) 711
PspGI CCWGG 4 cut(s) 752, 957, 1360, 1531
PspN4I GGNNCC 6 cut(s) 64, 645, 646, 1367, 1646, 2457
PspOMI GGGCCC 1 cut(s) 2455
PspPI GGNCC 8 cut(s) 408, 644, 1644, 1999, 2222, 2243, 2455, 2456
PspPPI RGGWCCY 1 cut(s) 644
PsrI GAACNNNNNNTAC 2 cut(s) 1358, 1390
PstI CTGCAG 1 cut(s) 2059
PsuI RGATCY 5 cut(s) 118, 352, 791, 1561, 1638
PsyI GACNNNGTC 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 1808
RsaI GTAC 4 cut(s) 80, 874, 1314, 2355
RsaNI GTAC 4 cut(s) 79, 873, 1313, 2354
RseI CAYNNNNRTG 2 cut(s) 1118, 1443
SaqAI TTAA 6 cut(s) 620, 900, 1142, 1749, 1842, 1886
Sau96I GGNCC 8 cut(s) 408, 644, 1644, 1999, 2222, 2243, 2455, 2456
ScaI AGTACT 2 cut(s) 1314, 2355
SchI GAGTC 4 cut(s) 199, 812, 1195, 2037
ScrFI CCNGG 4 cut(s) 754, 959, 1362, 1533
SduI GDGCHC 3 cut(s) 129, 871, 2459
SfaNI GCATC 8 cut(s) 854, 1447, 1466, 1499, 1937, 2145, 2155, 2349
SfcI CTRYAG 3 cut(s) 811, 1224, 2055
SfuI TTCGAA 2 cut(s) 211, 2474
SinI GGWCC 6 cut(s) 408, 644, 1644, 1999, 2222, 2243
SmiMI CAYNNNNRTG 2 cut(s) 1118, 1443
SmlI CTYRAG 2 cut(s) 29, 494
SmoI CTYRAG 2 cut(s) 29, 494
SpeI ACTAGT 1 cut(s) 1315
SphI GCATGC 2 cut(s) 1349, 1675
SseBI AGGCCT 1 cut(s) 469
SsiI CCGC 9 cut(s) 11, 17, 169, 350, 416, 842, 1006, 2110, 2173
SspI AATATT 1 cut(s) 1131
SspMI CTAG 4 cut(s) 1316, 1649, 1902, 2240
StuI AGGCCT 1 cut(s) 469
StyD4I CCNGG 4 cut(s) 752, 957, 1360, 1531
StyI CCWWGG 1 cut(s) 2091
TaaI ACNGT 3 cut(s) 866, 2188, 2481
TaqI TCGA 9 cut(s) 211, 237, 264, 336, 777, 1329, 1938, 2299, 2474
TaqII GACCGA 1 cut(s) 122
TatI WGTACW 2 cut(s) 1312, 2353
TauI GCSGC 4 cut(s) 14, 20, 418, 845
TfiI GAWTC 1 cut(s) 1265
Tru1I TTAA 6 cut(s) 620, 900, 1142, 1749, 1842, 1886
Tru9I TTAA 6 cut(s) 620, 900, 1142, 1749, 1842, 1886
TscAI CASTG 2 cut(s) 334, 1868
TseFI GTSAC 2 cut(s) 478, 804
TseI GCWGC 8 cut(s) 14, 46, 521, 727, 839, 1067, 1763, 1808
Tsp45I GTSAC 2 cut(s) 478, 804
TspDTI ATGAA 6 cut(s) 334, 600, 782, 1085, 1102, 2058
TspGWI ACGGA 1 cut(s) 1098
TspRI CASTG 2 cut(s) 334, 1868
Tth111I GACNNNGTC 1 cut(s) 242
Van91I CCANNNNNTGG 2 cut(s) 1361, 2083
VneI GTGCAC 1 cut(s) 867
VpaK11BI GGWCC 6 cut(s) 408, 644, 1644, 1999, 2222, 2243
XapI RAATTY 7 cut(s) 213, 323, 531, 705, 1589, 1744, 1821
XceI RCATGY 2 cut(s) 1349, 1675
XcmI CCANNNNNNNNNTGG 2 cut(s) 1142, 1475
XmiI GTMKAC 1 cut(s) 861
XmnI GAANNNNTTC 1 cut(s) 1856
XspI CTAG 4 cut(s) 1316, 1649, 1902, 2240
ZraI GACGTC 2 cut(s) 244, 1277
ZrmI AGTACT 2 cut(s) 1314, 2355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.