RLG00000027553

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005758
Physical Location & Seq
Reverse (-)
12645100 .. 12653171
8072 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027553

Sequence Viewer

Length: 2502 bp
ATGGCGACGGCGACGGCGGCTGCGGCGTTGGTTCTCAAGCCGTCGTGCGTGCTGCTACGGCCGAGGGGAGCCAAAACGACGACGTACAAGAGTAAGCGAGCAATAAAGGGACGAGCTTTGGGGGATTTAGGGCACTTCGGTCAGGTGGTCAGCAAGGATGTGGAGTTTCTGAAGCGGAGGATTGGTGGAGGATTTGAGTGGGCGAATAAGACTCTTCGGATTCCGGAGGTGTTCAAAGCCATCGACGACGTCGTTTGGCTTCGTAACCTCGAAGAACCCTATGCCCCTCCCTTGCCGGAGGCTCGTTGGCCTCAACCTTCCTATCCAGAATTCACTGGTGTCGATCTATTCATGGCGGATCTCAAAGCGTTAGAGGCATATGCTCTTTACTTTTATTATCTTTCTAAGAGTTGGTCCAAGCCGCTTCCTGAAGTCTATGATCCAGAAAGTGTTGCCGATTATTTCAGTTGCAGGCCTCACGTAGTCACCCTTAGACTTCTTGAGGTAGTTTCCTCCTTTGCTTTTGCTGCAATCAGAATTCGGACATCTGGGATCAAAAAGTATATACGACCAAGTTCGGGTGATGATTATGAAAGCTTATCACAATACAATTTTGGAATGGTGTTAAAAGAAACTATGCTAAACCTGGGGCCCACTTTTATCAAAGTTGGCCAGTCCCTTTCCACCAGACCAGACATTATTGGTGCTGAAATTTCCCAGCCATTGTCTGAGCTGCATGATCAAATCCCTCCTTTTTCCAGGGATGTGGCGATGAAAATTATCGAAGAAGAATTAGGATCTCCTGCTGAGTCACTCTATAGCTACATTTCTGAGAAACCTGAGGCAGCGGCATCTTTTGGTCAGGTCTACCGTGCACGTACTCATGATGGACTGGATGTTGCTGTTAAAGTTCAGCGTCCTAACTTGCGTCATATTGTAGTGCGCGATATATACATTCTTCGCCTTGGGCTTGGACTATTGCAAAAGATAGCAAAGAGAAAAGGTGATCTCCGCCTATATGCCGATGAACTTGGGAAAGGTTTTGTTGGAGAACTGGATTATACTTTAGAAGCTGCAAATGCTTCCAAGTTTCTGGAAGTTTATTCCTCCTATCCGTTCATGCGCACGCCAAAAGTATTCCAAAGTTTAAGTGGAAAGAGAGTTCTGACAATGGAGTGGATTGTTGGTGAGAGTCCAACCGACTTATTGTCTGTATCTTCTGGAAGCTCTAGAGATGATAGCTCTACATATACAGAGAGGCAAAGATTAGATTCCAAAAGGCGTCTCCTTGATCTGGTCAGGAAAGGGGTGGAGGCAGTACTAGTTCAGCTCCTCGAAACAGGATTATTGCATGCTGATCCACATCCTGGGAACCTACGTTACACATCTTCTGGACAAATAGGGTTTTTGGATTTTGGCCTGCTTTGTCAGATGGAAAAGAGGCATCGATATGCTATGCTTGCATCTATTGTCCACATTGTAAATGGAGATTGGGCATCCCTTGTCCAATGTTTGACTGAAATGGATGTTGTAAGACCAGGGACTAATATCAGACGTGTAACAATGGATCTGGAATATGACTTGGGCGAGGTAGAATTCAGAGATGGAATTCCTGATGTGAAGTTCAGCAGGGTTTTGGGTAAGATCTGGTCCCTAGCCTTCAAGTATCATTTTCGCATGCCACCCTACTACTCGCTTGTTCTACGTTCTCTTGCTTCCTTTGAAGGATTAGCCGTCGCTGGAGATAGAAATTTTAAGACATTTGAAGCTGCATACCCTTATGTCGTTCGGAAACTACTTACTGAAAACTCAGCTGCCACAAGGAAAATTTTGCATTCGGTAGTTTTTAACAAAAAGAAGGAGTTCCAGTGGCAAAAGCTTGCTCTTTTCTTAAAAGTAGGCGCAACTAGGAAAGGTTTGAATGGGAGTATAGCATCAAAACTCGACACTTCTCTTGATTATTTACCTGCAAGAGACAATGGTGAAATTGATGTTGCAAACTTGGTCCTGAAGCTTTTACCATCTAAAGATGGTGTTGTGCTGAGAAGACTCTTGATGACTGCAGATGGAGCTTCATTGATCCAAGCAATGGTATCCAAGGAAGCAAAGTTTTTCCGCCAGCAATTTTGCAGGTTTGTTGCAGACATTTTGCATCAATGGATGCTTGAAACGCTTGTGCGGGGCATTACAACAGTTCAGTATAGCTCTAATTTGAGAGTGGCGAGTGGACCTGACAACAAAGAACAAGTTCCATCTTCCAGACTATCTACTCCAATTTATGATTATCGCTCTATCCTCAGAGATCGACGGCTCAAAGTGATTTTCTCAAACATACTAAACTCTGCAAGGAAAAATCCAGTACTGATGCTGAGGTTCTGTTGGACTTCCTTTGTTATGTCTGTTGTGGCTTTAGCTCTGGCTTCTCATCGTGTTCTAATATCCTTCTCTGAAGCTTACTTGGGCCCAATATCTTTTGCTTCGAAACAGTATGCGATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1 APH DCP2 NUDIX

Protein Analysis

834

Amino Acids

93.79

Weight (kDa)

9.44

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 245 - 503 1.2e-67 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 252
Acc16I TGCGCA 1 cut(s) 1124
Acc36I ACCTGC 2 cut(s) 1975, 2121
AccB7I CCANNNNNTGG 2 cut(s) 1367, 2089
AccI GTMKAC 1 cut(s) 867
AccII CGCG 1 cut(s) 945
AccIII TCCGGA 1 cut(s) 223
AciI CCGC 9 cut(s) 17, 23, 175, 356, 422, 848, 1012, 2116, 2179
AclWI GGATC 8 cut(s) 366, 434, 560, 805, 1352, 1575, 2074, 2488
AcoI YGGCCR 2 cut(s) 59, 670
AcsI RAATTY 7 cut(s) 329, 537, 711, 1595, 1608, 1750, 1827
AcuI CTGAAG 4 cut(s) 191, 450, 2030, 2469
AcyI GRCGYC 2 cut(s) 249, 1282
AfaI GTAC 4 cut(s) 86, 880, 1320, 2361
AfiI CCNNNNNNNGG 4 cut(s) 578, 1294, 1367, 2089
AflIII ACRYGT 1 cut(s) 1555
AgsI TTSAA 6 cut(s) 235, 1663, 1724, 1766, 1921, 2168
AhdI GACNNNNNGTC 1 cut(s) 1207
AhlI ACTAGT 1 cut(s) 1321
AjiI CACGTC 1 cut(s) 1556
AjnI CCWGG 4 cut(s) 645, 758, 1366, 1537
AloI GAACNNNNNNTCC 2 cut(s) 1146, 1178
Alw21I GWGCWC 1 cut(s) 877
Alw26I GTCTC 2 cut(s) 1289, 1968
Alw44I GTGCAC 1 cut(s) 873
AlwI GGATC 8 cut(s) 366, 434, 560, 805, 1352, 1575, 2074, 2488
Aor13HI TCCGGA 1 cut(s) 223
AoxI GGCC 7 cut(s) 59, 308, 473, 650, 670, 1417, 2461
ApaI GGGCCC 2 cut(s) 654, 2465
ApaLI GTGCAC 1 cut(s) 873
ApeKI GCWGC 8 cut(s) 20, 52, 527, 733, 845, 1073, 1769, 1814
ApoI RAATTY 7 cut(s) 329, 537, 711, 1595, 1608, 1750, 1827
Asp700I GAANNNNTTC 2 cut(s) 1862, 2247
AspLEI GCGC 3 cut(s) 945, 1125, 1904
AspS9I GGNCC 8 cut(s) 414, 650, 651, 1650, 2005, 2228, 2461, 2462
AsuHPI GGTGA 5 cut(s) 478, 593, 1016, 1199, 1994
AsuII TTCGAA 1 cut(s) 2480
AvaII GGWCC 4 cut(s) 414, 1650, 2005, 2228
AxyI CCTNAGG 1 cut(s) 840
BaeGI GKGCMC 4 cut(s) 135, 654, 877, 2465
BalI TGGCCA 1 cut(s) 672
BanII GRGCYC 2 cut(s) 654, 2465
BbsI GAAGAC 1 cut(s) 2053
Bbv12I GWGCWC 1 cut(s) 877
BbvCI CCTCAGC 1 cut(s) 2369
BbvI GCAGC 8 cut(s) 7, 39, 514, 720, 857, 1060, 1756, 1801
BccI CCATC 8 cut(s) 248, 881, 1426, 1598, 2024, 2029, 2060, 2260
BceAI ACGGC 6 cut(s) 24, 25, 30, 74, 1718, 2324
BciT130I CCWGG 4 cut(s) 647, 760, 1368, 1539
BciVI GTATCC 1 cut(s) 2104
BclI TGATCA 1 cut(s) 739
BcoDI GTCTC 2 cut(s) 1289, 1968
BcuI ACTAGT 1 cut(s) 1321
BfaI CTAG 4 cut(s) 1230, 1322, 1655, 1908
BfmI CTRYAG 2 cut(s) 817, 2061
BfuAI ACCTGC 2 cut(s) 1975, 2121
BfuI GTATCC 1 cut(s) 2104
BglII AGATCT 1 cut(s) 1644
BmcAI AGTACT 2 cut(s) 1320, 2361
Bme1390I CCNGG 4 cut(s) 647, 760, 1368, 1539
Bme18I GGWCC 4 cut(s) 414, 1650, 2005, 2228
BmeRI GACNNNNNGTC 1 cut(s) 1207
BmgBI CACGTC 1 cut(s) 1556
BmgT120I GGNCC 8 cut(s) 414, 650, 651, 1650, 2005, 2228, 2461, 2462
BmiI GGNNCC 6 cut(s) 70, 651, 652, 1373, 1652, 2463
BmrFI CCNGG 4 cut(s) 647, 760, 1368, 1539
BmsI GCATC 8 cut(s) 860, 1453, 1472, 1505, 1943, 2151, 2161, 2355
BpiI GAAGAC 1 cut(s) 2053
BpmI CTGGAG 1 cut(s) 1761
Bpu10I CCTNAGC 1 cut(s) 2369
Bpu14I TTCGAA 1 cut(s) 2480
BpuEI CTTGAG 2 cut(s) 20, 521
Bsa29I ATCGAT 1 cut(s) 1447
BsaAI YACGTR 2 cut(s) 481, 878
BsaBI GATNNNNATC 1 cut(s) 1362
BsaHI GRCGYC 2 cut(s) 249, 1282
BsaJI CCNNGG 7 cut(s) 62, 646, 759, 964, 1367, 1538, 2097
BsaWI WCCGGW 1 cut(s) 223
BsaXI ACNNNNNCTCC 4 cut(s) 169, 199, 1853, 1883
Bsc4I CCNNNNNNNGG 4 cut(s) 578, 1294, 1367, 2089
Bse1I ACTGG 6 cut(s) 340, 673, 897, 1059, 1867, 2357
Bse21I CCTNAGG 1 cut(s) 840
Bse3DI GCAATG 1 cut(s) 2094
Bse8I GATNNNNATC 1 cut(s) 1362
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 4 cut(s) 647, 760, 1368, 1539
BseCI ATCGAT 1 cut(s) 1447
BseDI CCNNGG 7 cut(s) 62, 646, 759, 964, 1367, 1538, 2097
BseGI GGATG 7 cut(s) 163, 769, 901, 1363, 1496, 1531, 2166
BseJI GATNNNNATC 1 cut(s) 1362
BseLI CCNNNNNNNGG 4 cut(s) 578, 1294, 1367, 2089
BseMI GCAATG 1 cut(s) 2094
BseMII CTCAG 8 cut(s) 720, 798, 822, 831, 1824, 2033, 2311, 2360
BseNI ACTGG 6 cut(s) 340, 673, 897, 1059, 1867, 2357
BseRI GAGGAG 1 cut(s) 1322
BseSI GKGCMC 4 cut(s) 135, 654, 877, 2465
BseX3I CGGCCG 1 cut(s) 59
BseXI GCAGC 8 cut(s) 7, 39, 514, 720, 857, 1060, 1756, 1801
BseYI CCCAGC 1 cut(s) 717
Bsh1236I CGCG 1 cut(s) 945
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 7 cut(s) 61, 310, 475, 652, 672, 1419, 2463
BshVI ATCGAT 1 cut(s) 1447
BsiEI CGRYCG 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 877
BsiSI CCGG 2 cut(s) 224, 296
BslFI GGGAC 4 cut(s) 123, 661, 1555, 1636
BslI CCNNNNNNNGG 4 cut(s) 578, 1294, 1367, 2089
BsmAI GTCTC 2 cut(s) 1289, 1968
BsmBI CGTCTC 1 cut(s) 1289
BsmFI GGGAC 4 cut(s) 123, 661, 1555, 1636
BsmI GAATGC 1 cut(s) 1834
BsnI GGCC 7 cut(s) 61, 310, 475, 652, 672, 1419, 2463
Bsp119I TTCGAA 1 cut(s) 2480
Bsp120I GGGCCC 2 cut(s) 650, 2461
Bsp1286I GDGCHC 4 cut(s) 135, 654, 877, 2465
Bsp13I TCCGGA 1 cut(s) 223
BspACI CCGC 9 cut(s) 17, 23, 175, 356, 422, 848, 1012, 2116, 2179
BspANI GGCC 7 cut(s) 61, 310, 475, 652, 672, 1419, 2463
BspCNI CTCAG 8 cut(s) 721, 799, 823, 832, 1823, 2034, 2310, 2361
BspDI ATCGAT 1 cut(s) 1447
BspEI TCCGGA 1 cut(s) 223
BspFNI CGCG 1 cut(s) 945
BspHI TCATGA 1 cut(s) 883
BspLI GGNNCC 6 cut(s) 70, 651, 652, 1373, 1652, 2463
BspMAI CTGCAG 1 cut(s) 2065
BspMI ACCTGC 2 cut(s) 1975, 2121
BspPI GGATC 8 cut(s) 366, 434, 560, 805, 1352, 1575, 2074, 2488
BspT104I TTCGAA 1 cut(s) 2480
BsrDI GCAATG 1 cut(s) 2094
BsrI ACTGG 6 cut(s) 340, 673, 897, 1059, 1867, 2357
BssECI CCNNGG 7 cut(s) 62, 646, 759, 964, 1367, 1538, 2097
BssNI GRCGYC 2 cut(s) 249, 1282
BssT1I CCWWGG 2 cut(s) 964, 2097
Bst2UI CCWGG 4 cut(s) 647, 760, 1368, 1539
Bst4CI ACNGT 3 cut(s) 872, 2194, 2487
Bst6I CTCTTC 1 cut(s) 219
BstACI GRCGYC 2 cut(s) 249, 1282
BstBAI YACGTR 2 cut(s) 481, 878
BstBI TTCGAA 1 cut(s) 2480
BstF5I GGATG 7 cut(s) 163, 769, 901, 1363, 1496, 1531, 2166
BstFNI CGCG 1 cut(s) 945
BstHHI GCGC 3 cut(s) 945, 1125, 1904
BstMAI GTCTC 2 cut(s) 1289, 1968
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 9 cut(s) 17, 23, 58, 374, 527, 1079, 1460, 2069, 2170
BstNI CCWGG 4 cut(s) 647, 760, 1368, 1539
BstNSI RCATGY 2 cut(s) 1355, 1681
BstSCI CCNGG 4 cut(s) 645, 758, 1366, 1537
BstSFI CTRYAG 2 cut(s) 817, 2061
BstSLI GKGCMC 4 cut(s) 135, 654, 877, 2465
BstUI CGCG 1 cut(s) 945
BstV1I GCAGC 8 cut(s) 7, 39, 514, 720, 857, 1060, 1756, 1801
BstV2I GAAGAC 1 cut(s) 2053
BstX2I RGATCY 4 cut(s) 358, 797, 1567, 1644
BstXI CCANNNNNNTGG 2 cut(s) 766, 1093
BstYI RGATCY 4 cut(s) 358, 797, 1567, 1644
BstZI CGGCCG 1 cut(s) 59
Bsu15I ATCGAT 1 cut(s) 1447
Bsu36I CCTNAGG 1 cut(s) 840
BsuI GTATCC 1 cut(s) 2104
BsuRI GGCC 7 cut(s) 61, 310, 475, 652, 672, 1419, 2463
BsuTUI ATCGAT 1 cut(s) 1447
BtgZI GCGATG 1 cut(s) 785
BtrI CACGTC 1 cut(s) 1556
BtsCI GGATG 7 cut(s) 163, 769, 901, 1363, 1496, 1531, 2166
BtsIMutI CAGTG 2 cut(s) 333, 1874
BveI ACCTGC 2 cut(s) 1975, 2121
CciI TCATGA 1 cut(s) 883
CfoI GCGC 3 cut(s) 945, 1125, 1904
Cfr13I GGNCC 8 cut(s) 414, 650, 651, 1650, 2005, 2228, 2461, 2462
ClaI ATCGAT 1 cut(s) 1447
CseI GACGC 3 cut(s) 905, 917, 1271
Csp6I GTAC 4 cut(s) 85, 879, 1319, 2360
CviAII CATG 7 cut(s) 352, 737, 884, 1120, 1352, 1678, 2499
CviQI GTAC 4 cut(s) 85, 879, 1319, 2360
DriI GACNNNNNGTC 1 cut(s) 1207
EaeI YGGCCR 2 cut(s) 59, 670
EagI CGGCCG 1 cut(s) 59
Eam1104I CTCTTC 1 cut(s) 219
Eam1105I GACNNNNNGTC 1 cut(s) 1207
EarI CTCTTC 1 cut(s) 219
EciI GGCGGA 3 cut(s) 371, 1001, 2105
EclXI CGGCCG 1 cut(s) 59
Eco130I CCWWGG 2 cut(s) 964, 2097
Eco147I AGGCCT 1 cut(s) 475
Eco24I GRGCYC 2 cut(s) 654, 2465
Eco47I GGWCC 4 cut(s) 414, 1650, 2005, 2228
Eco52I CGGCCG 1 cut(s) 59
Eco57I CTGAAG 4 cut(s) 191, 450, 2030, 2469
Eco81I CCTNAGG 1 cut(s) 840
EcoO109I RGGNCCY 1 cut(s) 650
EcoRI GAATTC 4 cut(s) 329, 537, 1595, 1608
EcoRII CCWGG 4 cut(s) 645, 758, 1366, 1537
EcoT14I CCWWGG 2 cut(s) 964, 2097
EcoT38I GRGCYC 2 cut(s) 654, 2465
ErhI CCWWGG 2 cut(s) 964, 2097
Esp3I CGTCTC 1 cut(s) 1289
FaeI CATG 7 cut(s) 355, 740, 887, 1123, 1355, 1681, 2502
FaqI GGGAC 4 cut(s) 123, 661, 1555, 1636
FatI CATG 7 cut(s) 351, 736, 883, 1119, 1351, 1677, 2498
FauI CCCGC 1 cut(s) 2172
FauNDI CATATG 1 cut(s) 379
FbaI TGATCA 1 cut(s) 739
FblI GTMKAC 1 cut(s) 867
FokI GGATG 7 cut(s) 170, 776, 908, 1350, 1483, 1538, 2173
FriOI GRGCYC 2 cut(s) 654, 2465
FspAI RTGCGCAY 1 cut(s) 1124
FspBI CTAG 4 cut(s) 1230, 1322, 1655, 1908
FspI TGCGCA 1 cut(s) 1124
GlaI GCGC 3 cut(s) 944, 1124, 1903
GsaI CCCAGC 1 cut(s) 721
GsuI CTGGAG 1 cut(s) 1761
HaeIII GGCC 7 cut(s) 61, 310, 475, 652, 672, 1419, 2463
HapII CCGG 2 cut(s) 224, 296
HgaI GACGC 3 cut(s) 905, 917, 1271
HhaI GCGC 3 cut(s) 945, 1125, 1904
Hin1I GRCGYC 2 cut(s) 249, 1282
Hin1II CATG 7 cut(s) 355, 740, 887, 1123, 1355, 1681, 2502
Hin6I GCGC 3 cut(s) 943, 1123, 1902
HinP1I GCGC 3 cut(s) 943, 1123, 1902
HindIII AAGCTT 4 cut(s) 595, 1877, 2012, 2451
HinfI GANTC 6 cut(s) 211, 220, 809, 1192, 1271, 2049
HpaII CCGG 2 cut(s) 224, 296
HphI GGTGA 5 cut(s) 478, 593, 1016, 1199, 1994
Hpy166II GTNNAC 4 cut(s) 868, 875, 1474, 2228
Hpy8I GTNNAC 4 cut(s) 868, 875, 1474, 2228
HpyAV CCTTC 5 cut(s) 327, 1669, 1718, 1852, 2452
HpyCH4III ACNGT 3 cut(s) 872, 2194, 2487
HpyCH4IV ACGT 7 cut(s) 83, 249, 480, 877, 1378, 1555, 1705
HpyF10VI GCNNNNNNNGC 9 cut(s) 17, 23, 58, 374, 527, 1079, 1460, 2069, 2170
HpySE526I ACGT 7 cut(s) 83, 249, 480, 877, 1378, 1555, 1705
Hsp92I GRCGYC 2 cut(s) 249, 1282
Hsp92II CATG 7 cut(s) 355, 740, 887, 1123, 1355, 1681, 2502
HspAI GCGC 3 cut(s) 943, 1123, 1902
Kpn2I TCCGGA 1 cut(s) 223
Ksp22I TGATCA 1 cut(s) 739
LmnI GCTCC 3 cut(s) 68, 1335, 2069
Lsp1109I GCAGC 8 cut(s) 7, 39, 514, 720, 857, 1060, 1756, 1801
LweI GCATC 8 cut(s) 860, 1453, 1472, 1505, 1943, 2151, 2161, 2355
MaeI CTAG 4 cut(s) 1230, 1322, 1655, 1908
MaeII ACGT 7 cut(s) 83, 249, 480, 877, 1378, 1555, 1705
MaeIII GTNAC 5 cut(s) 263, 484, 810, 1379, 1558
MboII GAAGA 9 cut(s) 206, 284, 797, 800, 950, 1209, 1380, 2058, 2247
MflI RGATCY 4 cut(s) 358, 797, 1567, 1644
MhlI GDGCHC 4 cut(s) 135, 654, 877, 2465
MlsI TGGCCA 1 cut(s) 672
MluNI TGGCCA 1 cut(s) 672
MlyI GAGTC 4 cut(s) 205, 818, 1201, 2043
MmeI TCCRAC 3 cut(s) 1027, 1220, 2360
Mox20I TGGCCA 1 cut(s) 672
MroI TCCGGA 1 cut(s) 223
MroXI GAANNNNTTC 2 cut(s) 1862, 2247
MscI TGGCCA 1 cut(s) 672
MseI TTAA 6 cut(s) 626, 906, 1148, 1755, 1848, 1892
MslI CAYNNNNRTG 1 cut(s) 1449
Msp20I TGGCCA 1 cut(s) 672
MspA1I CMGCKG 2 cut(s) 848, 1814
MspI CCGG 2 cut(s) 224, 296
MspR9I CCNGG 4 cut(s) 647, 760, 1368, 1539
Mva1269I GAATGC 1 cut(s) 1834
MvaI CCWGG 4 cut(s) 647, 760, 1368, 1539
MvnI CGCG 1 cut(s) 945
MwoI GCNNNNNNNGC 9 cut(s) 17, 23, 58, 374, 527, 1079, 1460, 2069, 2170
NdeI CATATG 1 cut(s) 379
NlaIII CATG 7 cut(s) 355, 740, 887, 1123, 1355, 1681, 2502
NlaIV GGNNCC 6 cut(s) 70, 651, 652, 1373, 1652, 2463
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 2 cut(s) 484, 810
NsbI TGCGCA 1 cut(s) 1124
NspI RCATGY 2 cut(s) 1355, 1681
NspV TTCGAA 1 cut(s) 2480
PaeI GCATGC 2 cut(s) 1355, 1681
PagI TCATGA 1 cut(s) 883
PceI AGGCCT 1 cut(s) 475
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PctI GAATGC 1 cut(s) 1834
PdmI GAANNNNTTC 2 cut(s) 1862, 2247
PfeI GAWTC 2 cut(s) 220, 1271
PflFI GACNNNGTC 1 cut(s) 248
PflMI CCANNNNNTGG 2 cut(s) 1367, 2089
PleI GAGTC 4 cut(s) 205, 817, 1200, 2043
PpsI GAGTC 4 cut(s) 205, 817, 1200, 2043
Ppu21I YACGTR 2 cut(s) 481, 878
Psp6I CCWGG 4 cut(s) 645, 758, 1366, 1537
PspFI CCCAGC 1 cut(s) 717
PspGI CCWGG 4 cut(s) 645, 758, 1366, 1537
PspN4I GGNNCC 6 cut(s) 70, 651, 652, 1373, 1652, 2463
PspOMI GGGCCC 2 cut(s) 650, 2461
PspPI GGNCC 8 cut(s) 414, 650, 651, 1650, 2005, 2228, 2461, 2462
PsrI GAACNNNNNNTAC 2 cut(s) 1364, 1396
PstI CTGCAG 1 cut(s) 2065
PsuI RGATCY 4 cut(s) 358, 797, 1567, 1644
PsyI GACNNNGTC 1 cut(s) 248
PvuII CAGCTG 1 cut(s) 1814
RsaI GTAC 4 cut(s) 86, 880, 1320, 2361
RsaNI GTAC 4 cut(s) 85, 879, 1319, 2360
RseI CAYNNNNRTG 1 cut(s) 1449
SaqAI TTAA 6 cut(s) 626, 906, 1148, 1755, 1848, 1892
Sau96I GGNCC 8 cut(s) 414, 650, 651, 1650, 2005, 2228, 2461, 2462
ScaI AGTACT 2 cut(s) 1320, 2361
SchI GAGTC 4 cut(s) 205, 818, 1201, 2043
ScrFI CCNGG 4 cut(s) 647, 760, 1368, 1539
SduI GDGCHC 4 cut(s) 135, 654, 877, 2465
SfaNI GCATC 8 cut(s) 860, 1453, 1472, 1505, 1943, 2151, 2161, 2355
SfcI CTRYAG 2 cut(s) 817, 2061
SfuI TTCGAA 1 cut(s) 2480
SinI GGWCC 4 cut(s) 414, 1650, 2005, 2228
SmiMI CAYNNNNRTG 1 cut(s) 1449
SmlI CTYRAG 2 cut(s) 35, 500
SmoI CTYRAG 2 cut(s) 35, 500
SpeI ACTAGT 1 cut(s) 1321
SphI GCATGC 2 cut(s) 1355, 1681
SseBI AGGCCT 1 cut(s) 475
SsiI CCGC 9 cut(s) 17, 23, 175, 356, 422, 848, 1012, 2116, 2179
SspMI CTAG 4 cut(s) 1230, 1322, 1655, 1908
StuI AGGCCT 1 cut(s) 475
StyD4I CCNGG 4 cut(s) 645, 758, 1366, 1537
StyI CCWWGG 2 cut(s) 964, 2097
TaaI ACNGT 3 cut(s) 872, 2194, 2487
TaiI ACGT 7 cut(s) 86, 252, 483, 880, 1381, 1558, 1708
TaqI TCGA 9 cut(s) 243, 270, 342, 783, 1335, 1447, 1944, 2305, 2480
TaqII GACCGA 1 cut(s) 128
TatI WGTACW 2 cut(s) 1318, 2359
TauI GCSGC 4 cut(s) 20, 26, 424, 851
TfiI GAWTC 2 cut(s) 220, 1271
Tru1I TTAA 6 cut(s) 626, 906, 1148, 1755, 1848, 1892
Tru9I TTAA 6 cut(s) 626, 906, 1148, 1755, 1848, 1892
TscAI CASTG 2 cut(s) 340, 1874
TseFI GTSAC 2 cut(s) 484, 810
TseI GCWGC 8 cut(s) 20, 52, 527, 733, 845, 1073, 1769, 1814
Tsp45I GTSAC 2 cut(s) 484, 810
TspDTI ATGAA 6 cut(s) 340, 606, 788, 1041, 1108, 2064
TspGWI ACGGA 1 cut(s) 1104
TspRI CASTG 2 cut(s) 340, 1874
Tth111I GACNNNGTC 1 cut(s) 248
Van91I CCANNNNNTGG 2 cut(s) 1367, 2089
VneI GTGCAC 1 cut(s) 873
VpaK11BI GGWCC 4 cut(s) 414, 1650, 2005, 2228
XapI RAATTY 7 cut(s) 329, 537, 711, 1595, 1608, 1750, 1827
XbaI TCTAGA 1 cut(s) 1229
XceI RCATGY 2 cut(s) 1355, 1681
XcmI CCANNNNNNNNNTGG 2 cut(s) 1148, 1481
XmiI GTMKAC 1 cut(s) 867
XmnI GAANNNNTTC 2 cut(s) 1862, 2247
XspI CTAG 4 cut(s) 1230, 1322, 1655, 1908
ZraI GACGTC 1 cut(s) 250
ZrmI AGTACT 2 cut(s) 1320, 2361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.