pycom07g18690

Belongs to the adaptor complexes large subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009227
Physical Location & Seq
Reverse (-)
21103928 .. 21105365
1438 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1209 bp
ATGTTAGCTACAGTGCTCAAGTATCTTGCACGGTGCTTTGCTTCTGAAGAAGTGGAAACACAGCTTCAGATTTGTAATACCGCTGTTAAGCTTTTATTGGGGACAAAAGGGAATGGCCTGTTGACAATCAAAATGGTTTTAATCTACGTGTTGGAGCTGGCCCAATGCGACTTAAACTATGATGTTCGTGATCGAGCTCATTTCTTAAAGAAACTTCTTTCAACTTATCTAGATTCTCAAGGTTTGGAGGAGGAAACCAACTGTCCAGCCCAACATAAAGATAGTTCACGTCTCCTGGCAGAAATCTTGTTTGGAAAACAAAAGAAACCAGTGTCCCATGGGCCCATTGATCACTGGTTTTACCTTCCTGGCTCTTTATCGCAGATAGTACTTCATGCTGCTCCTGGGTATGAACCTCTCCCGAAGCCTTGTAGTTTGCGCAGTGATGGCCCCAGACTGAATGAATTTGGAGAGGCAGTCACTAATGGTGACCAATATGTTACAGACAGTGAGGACTCAGCTTCTGAATCTGTGGATGAGGAAGTTGCTTCTAGTTATAGTTCGCAACATTCTAATGTGGATTCAAGTGGCAGTGATGGCAGTGAAGAAGCTGGTTCTGTTAGTGAAGGTGATGATGATCCTCATCAGTTGATTCAGTTTTCAGATGAAAGAAAAAATGGAGCTTCTCAGTCTGCTTCTGATTTTGGGGAATTATTGTCAAATAGAGCTTTAGAATCATGGTTAGATGACCAACCTGGTTTTTCAAACCCAAATAATTCAGAACAAAGTCAAGTCCGTACATCTTCAGCAAGAATCTCCATCGGGGACTTCGGTGGTCAAGTTAGACGTAAAAGCTATCCACTCTTGGACCCTGTGAATGGAAACGGGTTAAAGGTTGACTATTCGTTTTCATCTGAGATATCAACTATCTCTCCTCGATTTATATGTATAGAAGTTTCCTTCAAGAACTGTTCAAAGGAGACCATGTCTGATGTAACTTTGGTTGACAAGGAGGCTGGCAAAGGCAAGGATTCTGTGGACCAAGCTTCGATTTCAGATGAAAGCTCTACGATACCTCAAAATAATGAGCCAAATCTGGTTTCTATAGAAGAAATCGCTTCTCTGGAACCTGGCCAGACAATGACAAGAAGCATCCAGGTGCGCTTCCATCACCACCTCTTACCTCTAAAACTGACCTTTATATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
AP3B1_C Adaptin_N

Protein Analysis

403

Amino Acids

44.09

Weight (kDa)

4.77

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP3B1_C PF14796 279 - 341 2.6e-09 Clathrin-adaptor complex-3 beta-1 subunit C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 440
AciI CCGC 1 cut(s) 81
AclWI GGATC 1 cut(s) 632
AcoI YGGCCR 1 cut(s) 1132
AcsI RAATTY 1 cut(s) 464
AcuI CTGAAG 3 cut(s) 50, 66, 789
AfaI GTAC 2 cut(s) 390, 799
AfiI CCNNNNNNNGG 1 cut(s) 878
AflIII ACRYGT 1 cut(s) 147
AgsI TTSAA 5 cut(s) 222, 585, 765, 964, 975
AjiI CACGTC 1 cut(s) 290
AjnI CCWGG 6 cut(s) 294, 367, 403, 754, 1129, 1155
AjuI GAANNNNNNNTTGG 4 cut(s) 294, 326, 1084, 1116
Alw21I GWGCWC 2 cut(s) 18, 199
Alw26I GTCTC 2 cut(s) 296, 974
AlwI GGATC 1 cut(s) 632
AlwNI CAGNNNCTG 1 cut(s) 524
AoxI GGCC 5 cut(s) 115, 159, 341, 448, 1132
ApaI GGGCCC 1 cut(s) 345
ApeKI GCWGC 1 cut(s) 398
ApoI RAATTY 1 cut(s) 464
AspLEI GCGC 2 cut(s) 441, 1164
AspS9I GGNCC 6 cut(s) 160, 341, 342, 449, 868, 1039
AsuHPI GGTGA 3 cut(s) 500, 641, 1163
AvaII GGWCC 2 cut(s) 868, 1039
BaeGI GKGCMC 1 cut(s) 345
BalI TGGCCA 1 cut(s) 1134
BanII GRGCYC 2 cut(s) 199, 345
Bbv12I GWGCWC 2 cut(s) 18, 199
BbvI GCAGC 1 cut(s) 385
BccI CCATC 4 cut(s) 440, 590, 827, 1176
BciT130I CCWGG 6 cut(s) 296, 369, 405, 756, 1131, 1157
BclI TGATCA 1 cut(s) 349
BcoDI GTCTC 2 cut(s) 296, 974
BfaI CTAG 2 cut(s) 230, 552
BfmI CTRYAG 2 cut(s) 9, 1104
BisI GCNGC 1 cut(s) 399
BlsI GCNGC 1 cut(s) 400
BmcAI AGTACT 1 cut(s) 390
Bme1390I CCNGG 6 cut(s) 296, 369, 405, 756, 1131, 1157
Bme18I GGWCC 2 cut(s) 868, 1039
BmgBI CACGTC 1 cut(s) 290
BmgT120I GGNCC 6 cut(s) 160, 341, 342, 449, 868, 1039
BmiI GGNNCC 4 cut(s) 343, 451, 870, 1128
BmrFI CCNGG 6 cut(s) 296, 369, 405, 756, 1131, 1157
BmsI GCATC 1 cut(s) 1161
BpuEI CTTGAG 1 cut(s) 222
BsaAI YACGTR 1 cut(s) 148
BsaBI GATNNNNATC 2 cut(s) 636, 642
BsaI GGTCTC 1 cut(s) 974
BsaJI CCNNGG 2 cut(s) 337, 404
BsaXI ACNNNNNCTCC 6 cut(s) 462, 492, 916, 946, 971, 1001
Bsc4I CCNNNNNNNGG 1 cut(s) 878
Bse1I ACTGG 2 cut(s) 329, 359
Bse8I GATNNNNATC 2 cut(s) 636, 642
BseBI CCWGG 6 cut(s) 296, 369, 405, 756, 1131, 1157
BseDI CCNNGG 2 cut(s) 337, 404
BseGI GGATG 2 cut(s) 541, 1152
BseJI GATNNNNATC 2 cut(s) 636, 642
BseLI CCNNNNNNNGG 1 cut(s) 878
BseMII CTCAG 3 cut(s) 531, 701, 906
BseNI ACTGG 2 cut(s) 329, 359
BseRI GAGGAG 2 cut(s) 263, 924
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 1 cut(s) 385
BshFI GGCC 5 cut(s) 117, 161, 343, 450, 1134
BsiHKAI GWGCWC 2 cut(s) 18, 199
BslFI GGGAC 3 cut(s) 115, 319, 839
BslI CCNNNNNNNGG 1 cut(s) 878
BsmAI GTCTC 2 cut(s) 296, 974
BsmBI CGTCTC 1 cut(s) 296
BsmFI GGGAC 3 cut(s) 115, 319, 839
BsnI GGCC 5 cut(s) 117, 161, 343, 450, 1134
Bso31I GGTCTC 1 cut(s) 974
Bsp120I GGGCCC 1 cut(s) 341
Bsp1286I GDGCHC 3 cut(s) 18, 199, 345
Bsp143I GATC 3 cut(s) 190, 349, 637
Bsp19I CCATGG 1 cut(s) 337
BspACI CCGC 1 cut(s) 81
BspANI GGCC 5 cut(s) 117, 161, 343, 450, 1134
BspCNI CTCAG 3 cut(s) 530, 700, 907
BspLI GGNNCC 4 cut(s) 343, 451, 870, 1128
BspPI GGATC 1 cut(s) 632
BspTNI GGTCTC 1 cut(s) 974
BsrI ACTGG 2 cut(s) 329, 359
BssECI CCNNGG 2 cut(s) 337, 404
BssMI GATC 3 cut(s) 190, 349, 637
BssT1I CCWWGG 1 cut(s) 337
Bst2UI CCWGG 6 cut(s) 296, 369, 405, 756, 1131, 1157
Bst4CI ACNGT 5 cut(s) 13, 33, 263, 509, 971
BstBAI YACGTR 1 cut(s) 148
BstC8I GCNNGC 2 cut(s) 159, 1018
BstDEI CTNAG 3 cut(s) 517, 687, 915
BstDSI CCRYGG 1 cut(s) 337
BstEII GGTNACC 1 cut(s) 488
BstF5I GGATG 2 cut(s) 541, 1152
BstHHI GCGC 2 cut(s) 441, 1164
BstKTI GATC 3 cut(s) 193, 352, 640
BstMAI GTCTC 2 cut(s) 296, 974
BstMBI GATC 3 cut(s) 190, 349, 637
BstMWI GCNNNNNNNGC 2 cut(s) 447, 597
BstNI CCWGG 6 cut(s) 296, 369, 405, 756, 1131, 1157
BstPI GGTNACC 1 cut(s) 488
BstSCI CCNGG 6 cut(s) 294, 367, 403, 754, 1129, 1155
BstSFI CTRYAG 2 cut(s) 9, 1104
BstSLI GKGCMC 1 cut(s) 345
BstV1I GCAGC 1 cut(s) 385
BsuRI GGCC 5 cut(s) 117, 161, 343, 450, 1134
BtgI CCRYGG 1 cut(s) 337
BtrI CACGTC 1 cut(s) 290
BtsCI GGATG 2 cut(s) 541, 1152
BtsI GCAGTG 3 cut(s) 448, 598, 607
BtsIMutI CAGTG 7 cut(s) 18, 336, 352, 448, 514, 598, 607
Cac8I GCNNGC 2 cut(s) 159, 1018
CaiI CAGNNNCTG 1 cut(s) 524
CfoI GCGC 2 cut(s) 441, 1164
Cfr13I GGNCC 6 cut(s) 160, 341, 342, 449, 868, 1039
CsiI ACCWGGT 1 cut(s) 754
Csp6I GTAC 2 cut(s) 389, 798
CviAII CATG 4 cut(s) 338, 395, 738, 985
CviQI GTAC 2 cut(s) 389, 798
DdeI CTNAG 3 cut(s) 517, 687, 915
DpnI GATC 3 cut(s) 192, 351, 639
DpnII GATC 3 cut(s) 190, 349, 637
EaeI YGGCCR 1 cut(s) 1132
Ecl136II GAGCTC 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 337
Eco24I GRGCYC 2 cut(s) 199, 345
Eco31I GGTCTC 1 cut(s) 974
Eco32I GATATC 1 cut(s) 921
Eco47I GGWCC 2 cut(s) 868, 1039
Eco53kI GAGCTC 1 cut(s) 197
Eco57I CTGAAG 3 cut(s) 50, 66, 789
Eco91I GGTNACC 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 197
EcoO65I GGTNACC 1 cut(s) 488
EcoRII CCWGG 6 cut(s) 294, 367, 403, 754, 1129, 1155
EcoRV GATATC 1 cut(s) 921
EcoT14I CCWWGG 1 cut(s) 337
EcoT38I GRGCYC 2 cut(s) 199, 345
ErhI CCWWGG 1 cut(s) 337
Esp3I CGTCTC 1 cut(s) 296
FaeI CATG 4 cut(s) 341, 398, 741, 988
FaqI GGGAC 3 cut(s) 115, 319, 839
FatI CATG 4 cut(s) 337, 394, 737, 984
FbaI TGATCA 1 cut(s) 349
Fnu4HI GCNGC 1 cut(s) 399
FokI GGATG 2 cut(s) 548, 1139
FriOI GRGCYC 2 cut(s) 199, 345
Fsp4HI GCNGC 1 cut(s) 399
FspBI CTAG 2 cut(s) 230, 552
FspI TGCGCA 1 cut(s) 440
GlaI GCGC 2 cut(s) 440, 1163
GluI GCNGC 1 cut(s) 399
HaeIII GGCC 5 cut(s) 117, 161, 343, 450, 1134
HhaI GCGC 2 cut(s) 441, 1164
Hin1II CATG 4 cut(s) 341, 398, 741, 988
Hin6I GCGC 2 cut(s) 439, 1162
HinP1I GCGC 2 cut(s) 439, 1162
HincII GTYRAC 3 cut(s) 123, 898, 1006
HindII GTYRAC 3 cut(s) 123, 898, 1006
HindIII AAGCTT 2 cut(s) 89, 1044
HinfI GANTC 8 cut(s) 233, 515, 527, 581, 652, 734, 813, 1031
HphI GGTGA 3 cut(s) 500, 641, 1163
Hpy166II GTNNAC 5 cut(s) 123, 287, 898, 1006, 1039
Hpy188I TCNGA 9 cut(s) 46, 69, 526, 664, 700, 781, 916, 991, 1057
Hpy188III TCNNGA 5 cut(s) 188, 230, 421, 964, 1124
Hpy8I GTNNAC 5 cut(s) 123, 287, 898, 1006, 1039
HpyAV CCTTC 3 cut(s) 374, 620, 970
HpyCH4III ACNGT 5 cut(s) 13, 33, 263, 509, 971
HpyCH4IV ACGT 3 cut(s) 147, 289, 847
HpyCH4V TGCA 1 cut(s) 29
HpyF10VI GCNNNNNNNGC 2 cut(s) 447, 597
HpyF3I CTNAG 3 cut(s) 517, 687, 915
HpySE526I ACGT 3 cut(s) 147, 289, 847
Hsp92II CATG 4 cut(s) 341, 398, 741, 988
HspAI GCGC 2 cut(s) 439, 1162
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 3 cut(s) 190, 349, 637
LmnI GCTCC 3 cut(s) 154, 406, 680
Lsp1109I GCAGC 1 cut(s) 385
LweI GCATC 1 cut(s) 1161
MabI ACCWGGT 1 cut(s) 754
MaeI CTAG 2 cut(s) 230, 552
MaeII ACGT 3 cut(s) 147, 289, 847
MaeIII GTNAC 4 cut(s) 478, 488, 499, 994
MalI GATC 3 cut(s) 192, 351, 639
MboI GATC 3 cut(s) 190, 349, 637
MboII GAAGA 4 cut(s) 59, 617, 795, 1121
MhlI GDGCHC 3 cut(s) 18, 199, 345
MlsI TGGCCA 1 cut(s) 1134
MluCI AATT 3 cut(s) 464, 710, 775
MluNI TGGCCA 1 cut(s) 1134
MlyI GAGTC 1 cut(s) 509
MmeI TCCRAC 1 cut(s) 132
Mox20I TGGCCA 1 cut(s) 1134
MscI TGGCCA 1 cut(s) 1134
MseI TTAA 5 cut(s) 87, 140, 173, 206, 890
MslI CAYNNNNRTG 2 cut(s) 573, 1157
Msp20I TGGCCA 1 cut(s) 1134
MspA1I CMGCKG 1 cut(s) 83
MspR9I CCNGG 6 cut(s) 296, 369, 405, 756, 1131, 1157
MvaI CCWGG 6 cut(s) 296, 369, 405, 756, 1131, 1157
MwoI GCNNNNNNNGC 2 cut(s) 447, 597
NcoI CCATGG 1 cut(s) 337
NdeII GATC 3 cut(s) 190, 349, 637
NlaIII CATG 4 cut(s) 341, 398, 741, 988
NlaIV GGNNCC 4 cut(s) 343, 451, 870, 1128
NmuCI GTSAC 2 cut(s) 478, 488
NsbI TGCGCA 1 cut(s) 440
PfeI GAWTC 7 cut(s) 233, 527, 581, 652, 734, 813, 1031
PflFI GACNNNGTC 1 cut(s) 985
PkrI GCNGC 1 cut(s) 400
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
Ppu21I YACGTR 1 cut(s) 148
Psp124BI GAGCTC 1 cut(s) 199
Psp6I CCWGG 6 cut(s) 294, 367, 403, 754, 1129, 1155
PspEI GGTNACC 1 cut(s) 488
PspGI CCWGG 6 cut(s) 294, 367, 403, 754, 1129, 1155
PspN4I GGNNCC 4 cut(s) 343, 451, 870, 1128
PspOMI GGGCCC 1 cut(s) 341
PspPI GGNCC 6 cut(s) 160, 341, 342, 449, 868, 1039
PstNI CAGNNNCTG 1 cut(s) 524
PsyI GACNNNGTC 1 cut(s) 985
RsaI GTAC 2 cut(s) 390, 799
RsaNI GTAC 2 cut(s) 389, 798
RseI CAYNNNNRTG 2 cut(s) 573, 1157
SacI GAGCTC 1 cut(s) 199
SaqAI TTAA 5 cut(s) 87, 140, 173, 206, 890
SatI GCNGC 1 cut(s) 399
Sau3AI GATC 3 cut(s) 190, 349, 637
Sau96I GGNCC 6 cut(s) 160, 341, 342, 449, 868, 1039
ScaI AGTACT 1 cut(s) 390
SchI GAGTC 1 cut(s) 509
ScrFI CCNGG 6 cut(s) 296, 369, 405, 756, 1131, 1157
SduI GDGCHC 3 cut(s) 18, 199, 345
SexAI ACCWGGT 1 cut(s) 754
SfaNI GCATC 1 cut(s) 1161
SfcI CTRYAG 2 cut(s) 9, 1104
SinI GGWCC 2 cut(s) 868, 1039
SmiMI CAYNNNNRTG 2 cut(s) 573, 1157
SmlI CTYRAG 2 cut(s) 17, 237
SmoI CTYRAG 2 cut(s) 17, 237
Sse9I AATT 3 cut(s) 464, 710, 775
SsiI CCGC 1 cut(s) 81
SspMI CTAG 2 cut(s) 230, 552
SstI GAGCTC 1 cut(s) 199
StyD4I CCNGG 6 cut(s) 294, 367, 403, 754, 1129, 1155
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 5 cut(s) 13, 33, 263, 509, 971
TaiI ACGT 3 cut(s) 150, 292, 850
TaqI TCGA 3 cut(s) 193, 937, 1049
TasI AATT 3 cut(s) 464, 710, 775
TatI WGTACW 1 cut(s) 388
TfiI GAWTC 7 cut(s) 233, 527, 581, 652, 734, 813, 1031
Tru1I TTAA 5 cut(s) 87, 140, 173, 206, 890
Tru9I TTAA 5 cut(s) 87, 140, 173, 206, 890
TscAI CASTG 7 cut(s) 18, 336, 359, 448, 514, 598, 607
TseFI GTSAC 2 cut(s) 478, 488
TseI GCWGC 1 cut(s) 398
Tsp45I GTSAC 2 cut(s) 478, 488
TspDTI ATGAA 6 cut(s) 383, 426, 477, 681, 900, 1074
TspGWI ACGGA 1 cut(s) 785
TspRI CASTG 7 cut(s) 18, 336, 359, 448, 514, 598, 607
Tth111I GACNNNGTC 1 cut(s) 985
VpaK11BI GGWCC 2 cut(s) 868, 1039
XapI RAATTY 1 cut(s) 464
XbaI TCTAGA 1 cut(s) 229
XspI CTAG 2 cut(s) 230, 552
ZrmI AGTACT 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.