pycom07g19190

DA1-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002660
Physical Location & Seq
Forward (+)
21514378 .. 21516168
1791 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1059 bp
ATGGGGACTTTCTTTCATCCCCAGTGCTTCTGTTGTCGTTCTTGTGGCCGTCCGATTGTTGAGAATGAGTTTTCTTCGTCAGGGAGGGATCCATATCACAAGTCCTGCTTTAAAGAACTGGCTCATCCCAAGTGTGAAGTTTGCCATCAATTTATTCCAACAAATCGAGCTGGTTTGATCGAGTACAGATGCCATCCGTTTTGGTCACAAAAGTACCGTCCAGCACATGAACAGGATAACACATCTCGTTGCTGCAGTTGCGAACGCTTAGAGTCTTGGGATGCAAGGTACGTTTCATTGGGGGACGGGCGAAGTCTATGCTTCGAGTGCATGGAATCTGCTATCATGGACACCGGTGACAGTCAGACTCTATATCACGCCATACGAGACTACTTTGAAGGGATGAACATGCAATTGGATCAGCAAATACCCATGCTTCTGGTCGAAAGACAAGGTCTAAAAGAAGCTATTGTAGGCGAAAAGAGTGGCCCCCATCACATGCCCGAGACGAGGGGTTTATGCCTTTTGGAAGAGCAGACTGTCACCAGTATCATCAAAAGGCCGAGAATTAGGGGGCATCAATATATTGGAATGAGAACCCAACCTCAGAAATTGACTCGAAAATGTGAAGTTACTGCCATTCTTGTTCTCTATGGCTTCCCAAGGTTACTAACAGGTGCCATTCTTGCCCATGAATTGATGCACGGCTTGTTACACCTCAAAGGGTACCAGAATCTTAATCCCGAGGTAGAGAAAGGCATCTGCCAGGTGCTTTCGTACATGTGGCTTGAGTCGGAAGTAATGCCGCGATTCAAAAACATGCCATCTTCATCTACATTTACATCCGCAGCTTCCTCTTCCTCTTCCACGTTTTCATCAAAAAAAGGTGGAAAATCGAAAATTGAACAAAAACTAGGGGAGTTTTTCATGCACCAAATCGCCAACGATTCTTCACCTGCATATGGAGGAGGGTTTCGAGCAGCTAATGCAGCTGTCAACAAGTATGGTTTGCGTCGTACATTGGATTACATTCGCCTCACCGGAAACTTCCCGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0007275 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0022622 GO:0032182 GO:0032501 GO:0032502 GO:0043130 GO:0048364 GO:0048731 GO:0048856 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

353

Amino Acids

39.95

Weight (kDa)

8.63

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 134 - 346 2.3e-90 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 964
Acc36I ACCTGC 1 cut(s) 964
Acc65I GGTACC 1 cut(s) 726
AccB1I GGYRCC 2 cut(s) 677, 726
AccII CGCG 1 cut(s) 808
AciI CCGC 2 cut(s) 806, 846
AclWI GGATC 3 cut(s) 83, 96, 426
AcoI YGGCCR 1 cut(s) 46
AfaI GTAC 6 cut(s) 185, 215, 290, 728, 779, 1018
AfiI CCNNNNNNNGG 2 cut(s) 510, 962
AflIII ACRYGT 1 cut(s) 780
AgeI ACCGGT 1 cut(s) 353
AgsI TTSAA 3 cut(s) 398, 814, 905
AjnI CCWGG 1 cut(s) 765
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
AluBI AGCT 5 cut(s) 170, 467, 851, 983, 992
AluI AGCT 5 cut(s) 170, 467, 851, 983, 992
Alw26I GTCTC 2 cut(s) 381, 500
AlwI GGATC 3 cut(s) 83, 96, 426
Ama87I CYCGRG 2 cut(s) 503, 743
AoxI GGCC 3 cut(s) 46, 487, 560
ApeKI GCWGC 4 cut(s) 252, 848, 980, 989
ArsI GACNNNNNNTTYG 2 cut(s) 202, 234
AsiGI ACCGGT 1 cut(s) 353
Asp718I GGTACC 1 cut(s) 726
AspS9I GGNCC 1 cut(s) 488
AsuHPI GGTGA 4 cut(s) 368, 535, 945, 1030
AvaI CYCGRG 2 cut(s) 503, 743
BaeI ACNNNNGTAYC 2 cut(s) 197, 230
BamHI GGATCC 1 cut(s) 88
BanI GGYRCC 2 cut(s) 677, 726
BbvI GCAGC 4 cut(s) 239, 860, 992, 1001
BccI CCATC 4 cut(s) 153, 201, 501, 832
BceAI ACGGC 2 cut(s) 33, 721
BcgI CGANNNNNNTGC 2 cut(s) 300, 334
BciT130I CCWGG 1 cut(s) 767
BcoDI GTCTC 2 cut(s) 381, 500
BfaI CTAG 1 cut(s) 914
BfmI CTRYAG 1 cut(s) 253
BfuAI ACCTGC 1 cut(s) 964
BisI GCNGC 5 cut(s) 253, 806, 849, 981, 990
BlsI GCNGC 5 cut(s) 254, 807, 850, 982, 991
Bme1390I CCNGG 1 cut(s) 767
BmeT110I CYCGRG 2 cut(s) 503, 743
BmgT120I GGNCC 1 cut(s) 488
BmiI GGNNCC 4 cut(s) 90, 490, 679, 728
BmrFI CCNGG 1 cut(s) 767
BmrI ACTGGG 1 cut(s) 16
BmsI GCATC 5 cut(s) 179, 271, 586, 690, 768
BmuI ACTGGG 1 cut(s) 16
BpuEI CTTGAG 1 cut(s) 809
BsaBI GATNNNNATC 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 662, 744
BsaWI WCCGGW 2 cut(s) 353, 1040
Bsc4I CCNNNNNNNGG 2 cut(s) 510, 962
Bse118I RCCGGY 1 cut(s) 353
Bse1I ACTGG 3 cut(s) 22, 123, 546
Bse8I GATNNNNATC 1 cut(s) 93
BseBI CCWGG 1 cut(s) 767
BseDI CCNNGG 2 cut(s) 662, 744
BseGI GGATG 6 cut(s) 16, 124, 193, 286, 408, 842
BseJI GATNNNNATC 1 cut(s) 93
BseLI CCNNNNNNNGG 2 cut(s) 510, 962
BseMII CTCAG 1 cut(s) 620
BseNI ACTGG 3 cut(s) 22, 123, 546
BseRI GAGGAG 1 cut(s) 981
BseXI GCAGC 4 cut(s) 239, 860, 992, 1001
Bsh1236I CGCG 1 cut(s) 808
BshFI GGCC 3 cut(s) 48, 489, 562
BshNI GGYRCC 2 cut(s) 677, 726
BshTI ACCGGT 1 cut(s) 353
BsiHKCI CYCGRG 2 cut(s) 503, 743
BsiSI CCGG 2 cut(s) 354, 1041
BslFI GGGAC 2 cut(s) 19, 317
BslI CCNNNNNNNGG 2 cut(s) 510, 962
BsmAI GTCTC 2 cut(s) 381, 500
BsmBI CGTCTC 1 cut(s) 500
BsmFI GGGAC 2 cut(s) 19, 317
BsnI GGCC 3 cut(s) 48, 489, 562
BsoBI CYCGRG 2 cut(s) 503, 743
Bsp143I GATC 3 cut(s) 88, 177, 418
BspACI CCGC 2 cut(s) 806, 846
BspANI GGCC 3 cut(s) 48, 489, 562
BspCNI CTCAG 1 cut(s) 619
BspFNI CGCG 1 cut(s) 808
BspLI GGNNCC 4 cut(s) 90, 490, 679, 728
BspMAI CTGCAG 1 cut(s) 257
BspMI ACCTGC 1 cut(s) 964
BspPI GGATC 3 cut(s) 83, 96, 426
BspQI GCTCTTC 1 cut(s) 525
BspT107I GGYRCC 2 cut(s) 677, 726
BsrFI RCCGGY 1 cut(s) 353
BsrI ACTGG 3 cut(s) 22, 123, 546
BssAI RCCGGY 1 cut(s) 353
BssECI CCNNGG 2 cut(s) 662, 744
BssMI GATC 3 cut(s) 88, 177, 418
BssT1I CCWWGG 1 cut(s) 662
Bst2UI CCWGG 1 cut(s) 767
Bst4CI ACNGT 3 cut(s) 218, 362, 541
Bst6I CTCTTC 3 cut(s) 525, 862, 868
BstAPI GCANNNNNTGC 1 cut(s) 986
BstDEI CTNAG 2 cut(s) 268, 606
BstF5I GGATG 6 cut(s) 16, 124, 193, 286, 408, 842
BstFNI CGCG 1 cut(s) 808
BstKTI GATC 3 cut(s) 91, 180, 421
BstMAI GTCTC 2 cut(s) 381, 500
BstMBI GATC 3 cut(s) 88, 177, 418
BstMWI GCNNNNNNNGC 5 cut(s) 258, 327, 686, 986, 989
BstNI CCWGG 1 cut(s) 767
BstNSI RCATGY 4 cut(s) 412, 502, 784, 823
BstSCI CCNGG 1 cut(s) 765
BstSFI CTRYAG 1 cut(s) 253
BstUI CGCG 1 cut(s) 808
BstV1I GCAGC 4 cut(s) 239, 860, 992, 1001
BstX2I RGATCY 1 cut(s) 88
BstXI CCANNNNNNTGG 1 cut(s) 439
BstYI RGATCY 1 cut(s) 88
BsuRI GGCC 3 cut(s) 48, 489, 562
BtsCI GGATG 6 cut(s) 16, 124, 193, 286, 408, 842
BtsIMutI CAGTG 1 cut(s) 29
BveI ACCTGC 1 cut(s) 964
Cfr10I RCCGGY 1 cut(s) 353
Cfr13I GGNCC 1 cut(s) 488
CseI GACGC 1 cut(s) 1001
Csp6I GTAC 6 cut(s) 184, 214, 289, 727, 778, 1017
CspAI ACCGGT 1 cut(s) 353
CviQI GTAC 6 cut(s) 184, 214, 289, 727, 778, 1017
DdeI CTNAG 2 cut(s) 268, 606
DpnI GATC 3 cut(s) 90, 179, 420
DpnII GATC 3 cut(s) 88, 177, 418
DraI TTTAAA 1 cut(s) 112
EaeI YGGCCR 1 cut(s) 46
Eam1104I CTCTTC 3 cut(s) 525, 862, 868
EarI CTCTTC 3 cut(s) 525, 862, 868
Eco130I CCWWGG 1 cut(s) 662
Eco88I CYCGRG 2 cut(s) 503, 743
EcoRII CCWGG 1 cut(s) 765
EcoT14I CCWWGG 1 cut(s) 662
ErhI CCWWGG 1 cut(s) 662
Esp3I CGTCTC 1 cut(s) 500
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FaqI GGGAC 2 cut(s) 19, 317
FauNDI CATATG 1 cut(s) 961
Fnu4HI GCNGC 5 cut(s) 253, 806, 849, 981, 990
FokI GGATG 6 cut(s) 3, 111, 180, 293, 415, 829
Fsp4HI GCNGC 5 cut(s) 253, 806, 849, 981, 990
FspBI CTAG 1 cut(s) 914
GluI GCNGC 5 cut(s) 253, 806, 849, 981, 990
HaeIII GGCC 3 cut(s) 48, 489, 562
HapII CCGG 2 cut(s) 354, 1041
HgaI GACGC 1 cut(s) 1001
HincII GTYRAC 1 cut(s) 997
HindII GTYRAC 1 cut(s) 997
HinfI GANTC 8 cut(s) 272, 335, 367, 616, 733, 791, 810, 947
HpaII CCGG 2 cut(s) 354, 1041
HphI GGTGA 4 cut(s) 368, 535, 945, 1030
Hpy166II GTNNAC 1 cut(s) 997
Hpy188I TCNGA 4 cut(s) 54, 366, 609, 796
Hpy188III TCNNGA 1 cut(s) 743
Hpy8I GTNNAC 1 cut(s) 997
Hpy99I CGWCG 1 cut(s) 1017
HpyAV CCTTC 1 cut(s) 392
HpyCH4III ACNGT 3 cut(s) 218, 362, 541
HpyCH4IV ACGT 2 cut(s) 291, 869
HpyCH4V TGCA 8 cut(s) 255, 284, 330, 412, 703, 931, 959, 989
HpyF10VI GCNNNNNNNGC 5 cut(s) 258, 327, 686, 986, 989
HpyF3I CTNAG 2 cut(s) 268, 606
HpySE526I ACGT 2 cut(s) 291, 869
KpnI GGTACC 1 cut(s) 730
Kzo9I GATC 3 cut(s) 88, 177, 418
LguI GCTCTTC 1 cut(s) 525
Lsp1109I GCAGC 4 cut(s) 239, 860, 992, 1001
LweI GCATC 5 cut(s) 179, 271, 586, 690, 768
MaeI CTAG 1 cut(s) 914
MaeII ACGT 2 cut(s) 291, 869
MaeIII GTNAC 6 cut(s) 204, 356, 541, 631, 666, 711
MalI GATC 3 cut(s) 90, 179, 420
MboI GATC 3 cut(s) 88, 177, 418
MboII GAAGA 6 cut(s) 66, 542, 819, 849, 855, 942
MfeI CAATTG 1 cut(s) 413
MflI RGATCY 1 cut(s) 88
MluCI AATT 6 cut(s) 149, 413, 567, 611, 695, 900
MlyI GAGTC 4 cut(s) 281, 361, 610, 800
MmeI TCCRAC 2 cut(s) 182, 774
MseI TTAA 2 cut(s) 111, 738
MspA1I CMGCKG 1 cut(s) 992
MspI CCGG 2 cut(s) 354, 1041
MspR9I CCNGG 1 cut(s) 767
MunI CAATTG 1 cut(s) 413
MvaI CCWGG 1 cut(s) 767
MvnI CGCG 1 cut(s) 808
MwoI GCNNNNNNNGC 5 cut(s) 258, 327, 686, 986, 989
NdeI CATATG 1 cut(s) 961
NdeII GATC 3 cut(s) 88, 177, 418
NlaIV GGNNCC 4 cut(s) 90, 490, 679, 728
NmeAIII GCCGAG 1 cut(s) 588
NmuCI GTSAC 3 cut(s) 204, 356, 541
NspI RCATGY 4 cut(s) 412, 502, 784, 823
PaqCI CACCTGC 1 cut(s) 964
PciI ACATGT 1 cut(s) 780
PciSI GCTCTTC 1 cut(s) 525
PfeI GAWTC 4 cut(s) 335, 733, 810, 947
PflFI GACNNNGTC 1 cut(s) 453
PinAI ACCGGT 1 cut(s) 353
PkrI GCNGC 5 cut(s) 254, 807, 850, 982, 991
PleI GAGTC 4 cut(s) 280, 361, 610, 799
PpsI GAGTC 4 cut(s) 280, 361, 610, 799
PscI ACATGT 1 cut(s) 780
Psp6I CCWGG 1 cut(s) 765
PspGI CCWGG 1 cut(s) 765
PspN4I GGNNCC 4 cut(s) 90, 490, 679, 728
PspPI GGNCC 1 cut(s) 488
PstI CTGCAG 1 cut(s) 257
PsuI RGATCY 1 cut(s) 88
PsyI GACNNNGTC 1 cut(s) 453
PvuII CAGCTG 1 cut(s) 992
RsaI GTAC 6 cut(s) 185, 215, 290, 728, 779, 1018
RsaNI GTAC 6 cut(s) 184, 214, 289, 727, 778, 1017
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 2 cut(s) 111, 738
SatI GCNGC 5 cut(s) 253, 806, 849, 981, 990
Sau3AI GATC 3 cut(s) 88, 177, 418
Sau96I GGNCC 1 cut(s) 488
SchI GAGTC 4 cut(s) 281, 361, 610, 800
ScrFI CCNGG 1 cut(s) 767
SfaNI GCATC 5 cut(s) 179, 271, 586, 690, 768
SfcI CTRYAG 1 cut(s) 253
SgrAI CRCCGGYG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 788
SmoI CTYRAG 1 cut(s) 788
Sse9I AATT 6 cut(s) 149, 413, 567, 611, 695, 900
SsiI CCGC 2 cut(s) 806, 846
SspMI CTAG 1 cut(s) 914
StyD4I CCNGG 1 cut(s) 765
StyI CCWWGG 1 cut(s) 662
TaaI ACNGT 3 cut(s) 218, 362, 541
TaiI ACGT 2 cut(s) 294, 872
TaqI TCGA 7 cut(s) 166, 180, 324, 444, 619, 896, 976
TasI AATT 6 cut(s) 149, 413, 567, 611, 695, 900
TatI WGTACW 1 cut(s) 183
TauI GCSGC 1 cut(s) 808
TfiI GAWTC 4 cut(s) 335, 733, 810, 947
Tru1I TTAA 2 cut(s) 111, 738
Tru9I TTAA 2 cut(s) 111, 738
TscAI CASTG 1 cut(s) 29
TseFI GTSAC 3 cut(s) 204, 356, 541
TseI GCWGC 4 cut(s) 252, 848, 980, 989
Tsp45I GTSAC 3 cut(s) 204, 356, 541
TspDTI ATGAA 8 cut(s) 5, 243, 285, 419, 708, 819, 864, 916
TspGWI ACGGA 1 cut(s) 186
TspRI CASTG 1 cut(s) 29
Tth111I GACNNNGTC 1 cut(s) 453
XceI RCATGY 4 cut(s) 412, 502, 784, 823
XspI CTAG 1 cut(s) 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.