pycom07g19790

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0011594
Physical Location & Seq
Reverse (-)
22062353 .. 22063090
738 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g19790.1

Sequence Viewer

Length: 738 bp
ATGGATTCCAGAATCGTCCCCTGTTCTAAGAGCTGGCAGAGCTCGCTTCAGATTTCGGCACCAATTTCTTCTTCGTTTTCGAAAGGGTCGTCGTTTTCGGTTTCTGTTCCTTCTGTGACGAAACCCAGTGGAAGAAGCAATGGAGCTCTGGGATGCGGCTTCGTTCGTTGGCTGAACTCTTCCGGGTCGGGCAATTCGAAACGTCAACGTACTTCGAACCAAGTTTGCAGAGCAAAGTTTGATGATTTTTCGTACGAAGAATTGTCGAACCAGATTGAGGAATTGGCGCAAGGGTTCAATCTTTCCGAGGACGATGACGAGAACGTCGACGAACCGGCTTTCTTGGCGTCGAAAACTTCAAAAATCTCTCCGATTTCGACTTACCCAGAAGGCGAAAACAGCAAAGGCAGCGGCTTTTCGATGCGGTTTACGAGCTTGAAGCTCAATGCATTGGAACCCTCTCTGCTGGGGATTCATCCGGAGCCGCCGGACTGGCCGGAGAGAAGCGAAATCGTCAGAGCCACCATGGAAAGAAAGGCCAACAGCCTCGAGCTTCCGGTGTCGCTCCGGTTAATCAAGAAGAAGCAGCGGCAGCAACGGGAGGAGGGTCCAAAAAGAGAAGCTGGGGAATTTACTTTTTGCTCCCTGAACAAGGCATTCTCTTCCATGGTGTTTATAATCCGTGAGCTCCATAGCCACGCTTTGAGTATAAGGGAAAGTTTGTACTCCTTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

27.21

Weight (kDa)

8.32

Isoelectric Point (pI)

50.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 677
AasI GACNNNNNNGTC 1 cut(s) 323
AccB1I GGYRCC 1 cut(s) 58
AccI GTMKAC 1 cut(s) 327
AccIII TCCGGA 1 cut(s) 478
AciI CCGC 5 cut(s) 156, 411, 424, 485, 589
AcoI YGGCCR 1 cut(s) 494
AcsI RAATTY 1 cut(s) 629
AcuI CTGAAG 1 cut(s) 32
AcyI GRCGYC 1 cut(s) 347
AfaI GTAC 3 cut(s) 211, 254, 725
AfiI CCNNNNNNNGG 2 cut(s) 277, 652
AgsI TTSAA 3 cut(s) 298, 360, 439
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AluBI AGCT 8 cut(s) 33, 42, 146, 435, 442, 553, 623, 688
AluI AGCT 8 cut(s) 33, 42, 146, 435, 442, 553, 623, 688
Alw21I GWGCWC 3 cut(s) 44, 148, 690
Ama87I CYCGRG 1 cut(s) 548
Aor13HI TCCGGA 1 cut(s) 478
AoxI GGCC 2 cut(s) 494, 537
ApeKI GCWGC 3 cut(s) 408, 586, 592
ApoI RAATTY 1 cut(s) 629
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 1 cut(s) 608
AsuC2I CCSGG 1 cut(s) 184
AsuII TTCGAA 3 cut(s) 80, 197, 215
AvaI CYCGRG 1 cut(s) 548
AvaII GGWCC 1 cut(s) 608
BanI GGYRCC 1 cut(s) 58
BanII GRGCYC 3 cut(s) 44, 148, 690
Bbv12I GWGCWC 3 cut(s) 44, 148, 690
BbvI GCAGC 3 cut(s) 420, 598, 604
BcnI CCSGG 1 cut(s) 184
BglI GCCNNNNNGGC 1 cut(s) 493
BisI GCNGC 7 cut(s) 157, 409, 412, 485, 587, 590, 593
BlsI GCNGC 7 cut(s) 158, 410, 413, 486, 588, 591, 594
Bme1390I CCNGG 1 cut(s) 184
Bme18I GGWCC 1 cut(s) 608
BmeT110I CYCGRG 1 cut(s) 548
BmgT120I GGNCC 1 cut(s) 608
BmiI GGNNCC 4 cut(s) 60, 456, 483, 609
BmrFI CCNGG 1 cut(s) 184
BmrI ACTGGG 1 cut(s) 120
BmsI GCATC 2 cut(s) 143, 411
BmuI ACTGGG 1 cut(s) 120
Bpu14I TTCGAA 3 cut(s) 80, 197, 215
BpuMI CCSGG 1 cut(s) 184
BsaHI GRCGYC 1 cut(s) 347
BsaJI CCNNGG 3 cut(s) 306, 525, 666
BsaWI WCCGGW 3 cut(s) 478, 556, 567
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 652
Bse118I RCCGGY 1 cut(s) 334
Bse1I ACTGG 2 cut(s) 126, 497
Bse3DI GCAATG 1 cut(s) 145
BseAI TCCGGA 1 cut(s) 478
BseDI CCNNGG 3 cut(s) 306, 525, 666
BseGI GGATG 2 cut(s) 158, 475
BseLI CCNNNNNNNGG 2 cut(s) 277, 652
BseMI GCAATG 1 cut(s) 145
BseNI ACTGG 2 cut(s) 126, 497
BseRI GAGGAG 1 cut(s) 617
BseXI GCAGC 3 cut(s) 420, 598, 604
BseYI CCCAGC 2 cut(s) 466, 623
BshFI GGCC 2 cut(s) 496, 539
BshNI GGYRCC 1 cut(s) 58
BsiHKAI GWGCWC 3 cut(s) 44, 148, 690
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 7 cut(s) 183, 335, 479, 488, 497, 557, 568
BsiWI CGTACG 1 cut(s) 252
BslFI GGGAC 1 cut(s) 2
BslI CCNNNNNNNGG 2 cut(s) 277, 652
BsmFI GGGAC 1 cut(s) 2
BsmI GAATGC 1 cut(s) 656
BsnI GGCC 2 cut(s) 496, 539
BsoBI CYCGRG 1 cut(s) 548
Bsp119I TTCGAA 3 cut(s) 80, 197, 215
Bsp1286I GDGCHC 3 cut(s) 44, 148, 690
Bsp13I TCCGGA 1 cut(s) 478
Bsp19I CCATGG 2 cut(s) 525, 666
BspACI CCGC 5 cut(s) 156, 411, 424, 485, 589
BspANI GGCC 2 cut(s) 496, 539
BspEI TCCGGA 1 cut(s) 478
BspLI GGNNCC 4 cut(s) 60, 456, 483, 609
BspT104I TTCGAA 3 cut(s) 80, 197, 215
BspT107I GGYRCC 1 cut(s) 58
BsrDI GCAATG 1 cut(s) 145
BsrFI RCCGGY 1 cut(s) 334
BsrI ACTGG 2 cut(s) 126, 497
BssAI RCCGGY 1 cut(s) 334
BssECI CCNNGG 3 cut(s) 306, 525, 666
BssNI GRCGYC 1 cut(s) 347
BssT1I CCWWGG 2 cut(s) 525, 666
Bst6I CTCTTC 2 cut(s) 184, 667
BstACI GRCGYC 1 cut(s) 347
BstBI TTCGAA 3 cut(s) 80, 197, 215
BstC8I GCNNGC 2 cut(s) 35, 44
BstDEI CTNAG 1 cut(s) 27
BstDSI CCRYGG 2 cut(s) 525, 666
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 2 cut(s) 158, 475
BstHHI GCGC 1 cut(s) 289
BstMWI GCNNNNNNNGC 7 cut(s) 39, 43, 344, 399, 408, 493, 592
BstSCI CCNGG 1 cut(s) 182
BstV1I GCAGC 3 cut(s) 420, 598, 604
BsuRI GGCC 2 cut(s) 496, 539
BtgI CCRYGG 2 cut(s) 525, 666
BtsCI GGATG 2 cut(s) 158, 475
BtsIMutI CAGTG 1 cut(s) 133
Cac8I GCNNGC 2 cut(s) 35, 44
CfoI GCGC 1 cut(s) 289
Cfr10I RCCGGY 1 cut(s) 334
Cfr13I GGNCC 1 cut(s) 608
CseI GACGC 1 cut(s) 336
Csp6I GTAC 3 cut(s) 210, 253, 724
CviAII CATG 2 cut(s) 526, 667
CviQI GTAC 3 cut(s) 210, 253, 724
DdeI CTNAG 1 cut(s) 27
DrdI GACNNNNNNGTC 1 cut(s) 323
DseDI GACNNNNNNGTC 1 cut(s) 323
EaeI YGGCCR 1 cut(s) 494
Eam1104I CTCTTC 2 cut(s) 184, 667
EarI CTCTTC 2 cut(s) 184, 667
Ecl136II GAGCTC 3 cut(s) 42, 146, 688
Eco130I CCWWGG 2 cut(s) 525, 666
Eco24I GRGCYC 3 cut(s) 44, 148, 690
Eco47I GGWCC 1 cut(s) 608
Eco53kI GAGCTC 3 cut(s) 42, 146, 688
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 548
EcoICRI GAGCTC 3 cut(s) 42, 146, 688
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoT14I CCWWGG 2 cut(s) 525, 666
EcoT22I ATGCAT 1 cut(s) 451
EcoT38I GRGCYC 3 cut(s) 44, 148, 690
ErhI CCWWGG 2 cut(s) 525, 666
FaeI CATG 2 cut(s) 529, 670
FaiI YATR 5 cut(s) 527, 668, 677, 693, 710
FaqI GGGAC 1 cut(s) 2
FatI CATG 2 cut(s) 525, 666
FblI GTMKAC 1 cut(s) 327
Fnu4HI GCNGC 7 cut(s) 157, 409, 412, 485, 587, 590, 593
FokI GGATG 2 cut(s) 165, 462
FriOI GRGCYC 3 cut(s) 44, 148, 690
Fsp4HI GCNGC 7 cut(s) 157, 409, 412, 485, 587, 590, 593
GlaI GCGC 1 cut(s) 288
GluI GCNGC 7 cut(s) 157, 409, 412, 485, 587, 590, 593
GsaI CCCAGC 2 cut(s) 470, 627
HaeIII GGCC 2 cut(s) 496, 539
HapII CCGG 7 cut(s) 183, 335, 479, 488, 497, 557, 568
HgaI GACGC 1 cut(s) 336
HhaI GCGC 1 cut(s) 289
Hin1I GRCGYC 1 cut(s) 347
Hin1II CATG 2 cut(s) 529, 670
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HincII GTYRAC 2 cut(s) 206, 328
HindII GTYRAC 2 cut(s) 206, 328
HinfI GANTC 3 cut(s) 5, 12, 472
HpaII CCGG 7 cut(s) 183, 335, 479, 488, 497, 557, 568
Hpy166II GTNNAC 3 cut(s) 206, 328, 429
Hpy188I TCNGA 4 cut(s) 51, 307, 372, 518
Hpy188III TCNNGA 3 cut(s) 9, 479, 577
Hpy8I GTNNAC 3 cut(s) 206, 328, 429
Hpy99I CGWCG 4 cut(s) 94, 329, 332, 352
HpyAV CCTTC 2 cut(s) 120, 383
HpyCH4IV ACGT 3 cut(s) 202, 208, 324
HpyCH4V TGCA 2 cut(s) 228, 449
HpyF10VI GCNNNNNNNGC 7 cut(s) 39, 43, 344, 399, 408, 493, 592
HpyF3I CTNAG 1 cut(s) 27
HpySE526I ACGT 3 cut(s) 202, 208, 324
Hsp92I GRCGYC 1 cut(s) 347
Hsp92II CATG 2 cut(s) 529, 670
HspAI GCGC 1 cut(s) 287
Kpn2I TCCGGA 1 cut(s) 478
LmnI GCTCC 5 cut(s) 143, 481, 570, 647, 693
Lsp1109I GCAGC 3 cut(s) 420, 598, 604
LweI GCATC 2 cut(s) 143, 411
MaeII ACGT 3 cut(s) 202, 208, 324
MaeIII GTNAC 1 cut(s) 115
MboII GAAGA 7 cut(s) 60, 63, 144, 171, 269, 592, 654
MhlI GDGCHC 3 cut(s) 44, 148, 690
MluCI AATT 5 cut(s) 63, 193, 260, 281, 629
MnlI CCTC 6 cut(s) 271, 301, 469, 557, 595, 598
Mph1103I ATGCAT 1 cut(s) 451
MroI TCCGGA 1 cut(s) 478
MseI TTAA 1 cut(s) 572
MspA1I CMGCKG 2 cut(s) 411, 589
MspI CCGG 7 cut(s) 183, 335, 479, 488, 497, 557, 568
MspR9I CCNGG 1 cut(s) 184
Mva1269I GAATGC 1 cut(s) 656
MwoI GCNNNNNNNGC 7 cut(s) 39, 43, 344, 399, 408, 493, 592
NciI CCSGG 1 cut(s) 184
NcoI CCATGG 2 cut(s) 525, 666
NlaIII CATG 2 cut(s) 529, 670
NlaIV GGNNCC 4 cut(s) 60, 456, 483, 609
NmuCI GTSAC 1 cut(s) 115
NsiI ATGCAT 1 cut(s) 451
NspV TTCGAA 3 cut(s) 80, 197, 215
PaeR7I CTCGAG 1 cut(s) 548
PcsI WCGNNNNNNNCGW 3 cut(s) 86, 194, 324
PctI GAATGC 1 cut(s) 656
PfeI GAWTC 3 cut(s) 5, 12, 472
Pfl23II CGTACG 1 cut(s) 252
PkrI GCNGC 7 cut(s) 158, 410, 413, 486, 588, 591, 594
PsiI TTATAA 1 cut(s) 677
Psp124BI GAGCTC 3 cut(s) 44, 148, 690
PspFI CCCAGC 2 cut(s) 466, 623
PspLI CGTACG 1 cut(s) 252
PspN4I GGNNCC 4 cut(s) 60, 456, 483, 609
PspPI GGNCC 1 cut(s) 608
PspXI VCTCGAGB 1 cut(s) 548
RsaI GTAC 3 cut(s) 211, 254, 725
RsaNI GTAC 3 cut(s) 210, 253, 724
SacI GAGCTC 3 cut(s) 44, 148, 690
SalI GTCGAC 1 cut(s) 326
SaqAI TTAA 1 cut(s) 572
SatI GCNGC 7 cut(s) 157, 409, 412, 485, 587, 590, 593
Sau96I GGNCC 1 cut(s) 608
ScrFI CCNGG 1 cut(s) 184
SduI GDGCHC 3 cut(s) 44, 148, 690
SfaNI GCATC 2 cut(s) 143, 411
Sfr274I CTCGAG 1 cut(s) 548
SfuI TTCGAA 3 cut(s) 80, 197, 215
SgrDI CGTCGACG 1 cut(s) 326
SinI GGWCC 1 cut(s) 608
SlaI CTCGAG 1 cut(s) 548
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
Sse9I AATT 5 cut(s) 63, 193, 260, 281, 629
SsiI CCGC 5 cut(s) 156, 411, 424, 485, 589
SstI GAGCTC 3 cut(s) 44, 148, 690
StyD4I CCNGG 1 cut(s) 182
StyI CCWWGG 2 cut(s) 525, 666
TaiI ACGT 3 cut(s) 205, 211, 327
TaqI TCGA 9 cut(s) 80, 197, 215, 266, 327, 350, 377, 419, 549
TasI AATT 5 cut(s) 63, 193, 260, 281, 629
TatI WGTACW 1 cut(s) 723
TauI GCSGC 4 cut(s) 159, 414, 487, 592
TfiI GAWTC 3 cut(s) 5, 12, 472
Tru1I TTAA 1 cut(s) 572
Tru9I TTAA 1 cut(s) 572
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 3 cut(s) 408, 586, 592
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 1 cut(s) 464
TspGWI ACGGA 1 cut(s) 671
TspRI CASTG 1 cut(s) 133
VpaK11BI GGWCC 1 cut(s) 608
XagI CCTNNNNNAGG 1 cut(s) 650
XapI RAATTY 1 cut(s) 629
XhoI CTCGAG 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 327
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.