pycom07g21170
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013923
Physical Location & Seq
Reverse (-)
22991954 .. 22995194
3241 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2919 bp
ATGCGACCGTTTTCAAGCATGAAACCGCTGCTCCTCTTCGCCGTCGTCGTTTTGGCTCCGGTTCTCGCGAGATCTCTGAACCCGTCTCTAAACGACGACGTGTTGGGTCTGATAGTTCTTAAGGCCGATATCCAAGATCCGAAGGGTAAGTTAGCGTCCTGGAGCGAAGTCGACAGCAGTCCTTGCAATTGGGTCGGGGTGAAATGCCACCCGAGATCCAACCGGGTCATTGAGCTCAGCCTCGACGACTTCTCGCTTTCGGGTCACATCGGCCGAGGCCTTCTGCAATTGCAAGCTCTTCGGAAGCTCTCTCTTTCGAAGAACAATCTCACCGGAAGCTTAACCCCTAACTTTACTCACATCGACAACCTCCGAGTCCTTGATTTGAGCGAGAACAGCTTCTCCGGTGGCGTTCCTGAAGAGTTATTTCGGCAATGTGGGTCGCTGAGAGTGATTTCTCTGGCGAAAAACAAGTTTTCCGGGAAAATTCCGGAGAGTTTGGGTTCTTGTGCGAGTCTAGCAGCTGTTAACTTTTCGTTGAACCAGTTTTCGGGTTCGGTTCCAGCTCGGGTTTGGTCCTTGAGTGGGCTTAGATCCCTGGATTTGTCTGATAACTTGTTGAAGGGTGAAATTCCAAAGGGGATTGAATTGAATAATTTGCGAGGAGTTAATTTGGCCAGGAATCGGTTTACCGGGCAACTTCCGGATGGAATTGGAAGCTGTTCGCTCTTGAGGTCCATTGATCTGAGTGAGAATTCGTTCTCGGGTAACCTCCCTCAGACAATGCAGAAACTTGGCCTGTGCAGCTATCTGAATCTGCACCAGAACGCATTTTCTGGTGAGGTTCCGGAGTGGATTGGCGAGATGAAAAGCCTGGAGACTTTGGACCTTTCTAGCAACCGGTTTACGGGTGAAGTTCCAAGCTCAATAGGCAATCTTGAGGCTTTGAAGGTATTGAATTTTTCTGCAAATGGGTTCACTGGGAGCTTGCCAAAGTCTATGGCTTATTGTACGAACCTTCTGGCTCTAGATTTTAGTAAGAATTCGATGGCGGGTGAACTTCCGGTGTGGATATTCGAGGCGGGTGCAGATGAAGTTTCACTTTCAGATAAAAAGCTAAGCGGAAGCAAGAACATCAATCAATCTTTATCAGCTGGAAATGCACTTCAGAATCTCCAAGTCTTGGATTTATCCCTTAATCATTTTTCGGGTGAAATTGCGTCCGACATTGGGGCCTTAAGCAGCTTGCATACTTTGAACCTTTCTGGTAACTCTCTTGCTGGTCCTATTCCTGTGGCTATTGGAGAGTTGAAGGTCCTGAACAATCTCGACTTGAGCGAGAATCGGCTCAATGGAAGCATCCCTCAGGAAATTGGAGGGGCTTTTTCTTTGAAGGAATTGAGATTGGATAAGAACTTCCTCACGGGAAAAATTCCAACTTCAATTGAGCATTGTTCTTCTCTGACCACTTTAACTGTATCACAGAACAGACTGACTGGTCCATTACCTGCAGCAATGTCCAAACTTACCAACTTACAAATTGTGGACTTGTCTTTCAATAACCTCACGGGAGGCCTGCCGAAGCAGTTAGCCAATCTTCCCAACCTACTTTCCTTCAACATTTCCCACAACAACCTCCAGGGTGAACTACCTACGGGTGCCTTTTTCAATACTATCTCCCCTTCCTCCGTCTCTGGAAATCCATCTCTCTGTGGCTCTGCAGTTAATAAGTCTTGCCCAGGAGTCCTTCCCAAACCCATTGTACTCAATCCCAATTCCTCTTCCGACTCCACCACAGGAGAAATCTCATCAAATCTTGGTCATAGAAGAATCTTACTCAGTATTTCTTCGCTTATTGCCATTGCTGCAGCTGCTGTCATTGTCATTGGTGTAATTGCCATTACTGTGCTTAATCTCCGTGTCCGATCTCCCACAACTCAATCGGCGCCAGCCCTCACATTTTCAGGTGGGGATGACTTCAGCCGTTCCCCAACAACAGATGGCAACTCTGGCAAGCTTGTCATGTTTTCAGGTGAGCCTGATTTCAGCACTGGTGCACATGCTCTGCTCAACAAGGACTGTGAGCTTGGTCGTGGCGGGTTTGGAGCAGTCTACCGGACACTTCTTCAAGATGGACGGCCAGTTGCAATCAAGAAGCTCACTGTTTCTAGTCTTGTCAAGTCTCAAGAAGAATTCGAAAGGGAAGTGAATAAATTGGGGAAAGTGAGGCACGATAATCTTGTGGAGATCGAAGGCTATTACTGGACTCCATCATTACAGCTCATCATATACGATTATGTTGCCGGTGGTAGTTTATATAAGCATCTTCATGATGAAGCAGGTGGAAACTTCCTGTCGTGGAATGATCGGTTCAATATAATTCTTGGAACTGCAAAAGGTTTGGCTCACTTGCATCAGATGAACATAATTCACTACAATATAAAGTCCAGTAATGTCCTGATTGGCTGCTCAGGCGAACCGAAAGTGGGAGACTTTGGTCTAGCGAGGTTGTTGCCAATGCTTGACCGATATGTTTTGAGCAGCAAGATCCAGAGTGCACTCGGCTACATGGCACCTGAGTTTGCTTGCAAAACAGTAAAGATAACTGAGAAATGTGATGTGTACGGGTTTGGGGTTTTGGTTCTAGAGATTGTCACCGGGAAAAGACCAGTCGAGTACATGGAAGATGATGTTGTGGTGCTCTGTGACATGGTGAGAGGAGCACTGGAAGAAGGCAGGGTAGAAGAATGCGTTGATGCTCGGCTCCAAGGAATTTTCCCAGCAGAAGAGGCTATACCTGTGATGAAACTAGGCTTGATATGCACATCACAAGTGCCGTCAAACAGACCAGACATGGGGGAGGTTGTCAACATTTTGGAGCTGATCCGATGTCCTTCAGAAGGCCAAGAGGAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

973

Amino Acids

104.38

Weight (kDa)

5.97

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 69 5.7e-12 Leucine rich repeat N-terminal domain
LRR_8 PF13855 99 - 159 8.1e-07 Leucine rich repeat
LRR_14 PF23598 217 - 298 3.6e-06 Leucine-rich repeat region
LRR_8 PF13855 291 - 349 1e-05 Leucine rich repeat
LRR_14 PF23598 386 - 546 7.8e-12 Leucine-rich repeat region
LRR_8 PF13855 463 - 522 1.6e-07 Leucine rich repeat
LRR_8 PF13855 487 - 546 1.1e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 692 - 957 1.6e-41 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 693 - 896 3.4e-38 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013923)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56370
fragaria_vesca FvH4_7g25950
malus_domestica MD01G1167900.v1.1 MD07G1233900.v1.1
prunus_persica Prupe.2G264300_v2.0.a1
pyrus_communis pycom01g18090 pycom07g21170
rosa_chinensis RchiOBHm_Chr1g0372331
rosa_laevigata RLG00000026907
rosa_multiflora Rmu_sc0001755.1_g000008
rosa_roxburghii Rroxscaffold_4G00285050
rosa_rugosa Rorug01G0371200
rosa_samantha Rh1AG379600 Rh1BG343100 Rh1CG356600 Rh1DG374300
rosa_wichuraiana Rw1G033370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2330
AasI GACNNNNNNGTC 1 cut(s) 1497
Acc36I ACCTGC 2 cut(s) 1516, 2330
AccB1I GGYRCC 3 cut(s) 1658, 1945, 2572
AccB7I CCANNNNNTGG 1 cut(s) 1183
AccI GTMKAC 2 cut(s) 171, 2112
AccII CGCG 1 cut(s) 68
AccIII TCCGGA 3 cut(s) 490, 703, 847
AciI CCGC 5 cut(s) 26, 1052, 1082, 1122, 2097
AclWI GGATC 5 cut(s) 131, 210, 588, 2542, 2878
AcoI YGGCCR 3 cut(s) 271, 675, 2138
AcsI RAATTY 8 cut(s) 486, 630, 754, 958, 1042, 1431, 2192, 2772
AcuI CTGAAG 4 cut(s) 438, 1151, 1963, 2880
AcyI GRCGYC 1 cut(s) 1946
AfaI GTAC 4 cut(s) 1012, 1764, 2624, 2678
AfiI CCNNNNNNNGG 9 cut(s) 550, 585, 684, 907, 1183, 1230, 2486, 2855, 2900
AflII CTTAAG 2 cut(s) 119, 1237
AflIII ACRYGT 1 cut(s) 99
AgeI ACCGGT 1 cut(s) 900
AjiI CACGTC 1 cut(s) 100
AjnI CCWGG 6 cut(s) 158, 597, 677, 873, 1638, 1738
AloI GAACNNNNNNTCC 2 cut(s) 386, 418
Alw21I GWGCWC 5 cut(s) 237, 2059, 2560, 2703, 2725
Alw26I GTCTC 5 cut(s) 90, 872, 1696, 2187, 2484
Alw44I GTGCAC 2 cut(s) 2055, 2556
AlwI GGATC 5 cut(s) 131, 210, 588, 2542, 2878
AlwNI CAGNNNCTG 1 cut(s) 1874
Ama87I CYCGRG 3 cut(s) 211, 567, 763
Aor13HI TCCGGA 3 cut(s) 490, 703, 847
AoxI GGCC 9 cut(s) 123, 271, 277, 675, 796, 1233, 1573, 2138, 2902
ApaLI GTGCAC 2 cut(s) 2055, 2556
ApoI RAATTY 8 cut(s) 486, 630, 754, 958, 1042, 1431, 2192, 2772
AsiGI ACCGGT 1 cut(s) 900
Asp700I GAANNNNTTC 3 cut(s) 398, 721, 758
AspLEI GCGC 1 cut(s) 1948
AspS9I GGNCC 7 cut(s) 576, 735, 886, 1233, 1283, 1315, 1499
AsuC2I CCSGG 4 cut(s) 224, 481, 694, 2659
AsuII TTCGAA 2 cut(s) 317, 2196
AvaI CYCGRG 3 cut(s) 211, 567, 763
AvaII GGWCC 6 cut(s) 576, 735, 886, 1283, 1315, 1499
AxyI CCTNAGG 1 cut(s) 1365
BaeGI GKGCMC 2 cut(s) 2059, 2560
BalI TGGCCA 1 cut(s) 677
BanI GGYRCC 3 cut(s) 1658, 1945, 2572
BanII GRGCYC 1 cut(s) 237
BarI GAAGNNNNNNTAC 2 cut(s) 2778, 2810
Bbv12I GWGCWC 5 cut(s) 237, 2059, 2560, 2703, 2725
BccI CCATC 6 cut(s) 701, 1042, 1711, 1994, 2126, 2278
BceAI ACGGC 4 cut(s) 26, 1968, 2153, 2821
BciT130I CCWGG 6 cut(s) 160, 599, 679, 875, 1640, 1740
BcnI CCSGG 4 cut(s) 224, 481, 694, 2659
BcoDI GTCTC 5 cut(s) 90, 872, 1696, 2187, 2484
BfaI CTAG 7 cut(s) 518, 894, 1028, 2169, 2501, 2645, 2810
BfmI CTRYAG 3 cut(s) 1509, 1719, 1866
BfoI RGCGCY 1 cut(s) 1949
BfrI CTTAAG 2 cut(s) 119, 1237
BfuAI ACCTGC 2 cut(s) 1516, 2330
BglII AGATCT 1 cut(s) 71
BlpI GCTNAGC 2 cut(s) 236, 1118
Bme18I GGWCC 6 cut(s) 576, 735, 886, 1283, 1315, 1499
BmeT110I CYCGRG 3 cut(s) 211, 567, 763
BmgBI CACGTC 1 cut(s) 100
BmgT120I GGNCC 7 cut(s) 576, 735, 886, 1233, 1283, 1315, 1499
BmiI GGNNCC 8 cut(s) 57, 561, 846, 1234, 1660, 1947, 2574, 2765
BmrI ACTGGG 1 cut(s) 990
BmsI GCATC 4 cut(s) 1368, 2332, 2422, 2746
BmuI ACTGGG 1 cut(s) 990
BoxI GACNNNNGTC 2 cut(s) 177, 2496
BpmI CTGGAG 3 cut(s) 181, 896, 1622
Bpu10I CCTNAGC 1 cut(s) 2470
Bpu1102I GCTNAGC 2 cut(s) 236, 1118
Bpu14I TTCGAA 2 cut(s) 317, 2196
BpuEI CTTGAG 5 cut(s) 601, 751, 959, 1354, 2169
BpuMI CCSGG 4 cut(s) 224, 481, 694, 2659
BsaHI GRCGYC 1 cut(s) 1946
BsaJI CCNNGG 5 cut(s) 274, 597, 1639, 1738, 2767
BsaWI WCCGGW 9 cut(s) 58, 332, 404, 490, 703, 847, 900, 1063, 2115
BsaXI ACNNNNNCTCC 4 cut(s) 15, 45, 386, 416
Bsc4I CCNNNNNNNGG 9 cut(s) 550, 585, 684, 907, 1183, 1230, 2486, 2855, 2900
Bse118I RCCGGY 2 cut(s) 900, 2303
Bse1I ACTGG 9 cut(s) 544, 985, 1501, 2056, 2141, 2267, 2448, 2669, 2730
Bse21I CCTNAGG 1 cut(s) 1365
Bse3DI GCAATG 3 cut(s) 440, 1521, 1860
BseAI TCCGGA 3 cut(s) 490, 703, 847
BseBI CCWGG 6 cut(s) 160, 599, 679, 875, 1640, 1740
BseDI CCNNGG 5 cut(s) 274, 597, 1639, 1738, 2767
BseGI GGATG 3 cut(s) 712, 1359, 1978
BseLI CCNNNNNNNGG 9 cut(s) 550, 585, 684, 907, 1183, 1230, 2486, 2855, 2900
BseMI GCAATG 3 cut(s) 440, 1521, 1860
BseMII CTCAG 9 cut(s) 250, 437, 737, 791, 1379, 1852, 2484, 2568, 2598
BseNI ACTGG 9 cut(s) 544, 985, 1501, 2056, 2141, 2267, 2448, 2669, 2730
BseRI GAGGAG 3 cut(s) 23, 678, 2733
BseSI GKGCMC 2 cut(s) 2059, 2560
BseX3I CGGCCG 1 cut(s) 271
BseYI CCCAGC 1 cut(s) 2779
BsgI GTGCAG 3 cut(s) 803, 823, 1107
Bsh1236I CGCG 1 cut(s) 68
Bsh1285I CGRYCG 2 cut(s) 8, 274
BshFI GGCC 9 cut(s) 125, 273, 279, 677, 798, 1235, 1575, 2140, 2904
BshNI GGYRCC 3 cut(s) 1658, 1945, 2572
BshTI ACCGGT 1 cut(s) 900
BsiEI CGRYCG 2 cut(s) 8, 274
BsiHKAI GWGCWC 5 cut(s) 237, 2059, 2560, 2703, 2725
BsiHKCI CYCGRG 3 cut(s) 211, 567, 763
BslI CCNNNNNNNGG 9 cut(s) 550, 585, 684, 907, 1183, 1230, 2486, 2855, 2900
BsmAI GTCTC 5 cut(s) 90, 872, 1696, 2187, 2484
BsmBI CGTCTC 2 cut(s) 90, 1696
BsmI GAATGC 1 cut(s) 2753
BsnI GGCC 9 cut(s) 125, 273, 279, 677, 798, 1235, 1575, 2140, 2904
BsoBI CYCGRG 3 cut(s) 211, 567, 763
Bsp119I TTCGAA 2 cut(s) 317, 2196
Bsp1286I GDGCHC 5 cut(s) 237, 2059, 2560, 2703, 2725
Bsp13I TCCGGA 3 cut(s) 490, 703, 847
Bsp1720I GCTNAGC 2 cut(s) 236, 1118
Bsp68I TCGCGA 1 cut(s) 68
BspACI CCGC 5 cut(s) 26, 1052, 1082, 1122, 2097
BspANI GGCC 9 cut(s) 125, 273, 279, 677, 798, 1235, 1575, 2140, 2904
BspCNI CTCAG 9 cut(s) 249, 438, 738, 790, 1378, 1851, 2483, 2569, 2599
BspEI TCCGGA 3 cut(s) 490, 703, 847
BspFNI CGCG 1 cut(s) 68
BspHI TCATGA 1 cut(s) 2329
BspLI GGNNCC 8 cut(s) 57, 561, 846, 1234, 1660, 1947, 2574, 2765
BspMAI CTGCAG 3 cut(s) 1513, 1723, 1870
BspMI ACCTGC 2 cut(s) 1516, 2330
BspPI GGATC 5 cut(s) 131, 210, 588, 2542, 2878
BspQI GCTCTTC 1 cut(s) 303
BspT104I TTCGAA 2 cut(s) 317, 2196
BspT107I GGYRCC 3 cut(s) 1658, 1945, 2572
BspTI CTTAAG 2 cut(s) 119, 1237
BsrDI GCAATG 3 cut(s) 440, 1521, 1860
BsrFI RCCGGY 2 cut(s) 900, 2303
BsrI ACTGG 9 cut(s) 544, 985, 1501, 2056, 2141, 2267, 2448, 2669, 2730
BssAI RCCGGY 2 cut(s) 900, 2303
BssECI CCNNGG 5 cut(s) 274, 597, 1639, 1738, 2767
BssNI GRCGYC 1 cut(s) 1946
BssT1I CCWWGG 1 cut(s) 2767
Bst2UI CCWGG 6 cut(s) 160, 599, 679, 875, 1640, 1740
Bst4CI ACNGT 6 cut(s) 9, 1477, 1906, 2081, 2164, 2596
Bst6I CTCTTC 5 cut(s) 41, 303, 414, 1786, 2781
BstACI GRCGYC 1 cut(s) 1946
BstAFI CTTAAG 2 cut(s) 119, 1237
BstAPI GCANNNNNTGC 1 cut(s) 183
BstBI TTCGAA 2 cut(s) 317, 2196
BstC8I GCNNGC 7 cut(s) 294, 989, 1247, 1577, 1950, 2015, 2587
BstEII GGTNACC 1 cut(s) 767
BstENI CCTNNNNNAGG 1 cut(s) 2898
BstF5I GGATG 3 cut(s) 712, 1359, 1978
BstFNI CGCG 1 cut(s) 68
BstH2I RGCGCY 1 cut(s) 1949
BstHHI GCGC 1 cut(s) 1948
BstMAI GTCTC 5 cut(s) 90, 872, 1696, 2187, 2484
BstMCI CGRYCG 2 cut(s) 8, 274
BstNI CCWGG 6 cut(s) 160, 599, 679, 875, 1640, 1740
BstNSI RCATGY 1 cut(s) 2063
BstPAI GACNNNNGTC 2 cut(s) 177, 2496
BstPI GGTNACC 1 cut(s) 767
BstSFI CTRYAG 3 cut(s) 1509, 1719, 1866
BstSLI GKGCMC 2 cut(s) 2059, 2560
BstUI CGCG 1 cut(s) 68
BstX2I RGATCY 5 cut(s) 71, 136, 215, 593, 2547
BstYI RGATCY 5 cut(s) 71, 136, 215, 593, 2547
BstZI CGGCCG 1 cut(s) 271
Bsu36I CCTNAGG 1 cut(s) 1365
BsuRI GGCC 9 cut(s) 125, 273, 279, 677, 798, 1235, 1575, 2140, 2904
BtrI CACGTC 1 cut(s) 100
BtsCI GGATG 3 cut(s) 712, 1359, 1978
BtsIMutI CAGTG 4 cut(s) 978, 2049, 2160, 2723
BtuMI TCGCGA 1 cut(s) 68
BveI ACCTGC 2 cut(s) 1516, 2330
Cac8I GCNNGC 7 cut(s) 294, 989, 1247, 1577, 1950, 2015, 2587
CaiI CAGNNNCTG 1 cut(s) 1874
CciI TCATGA 1 cut(s) 2329
CfoI GCGC 1 cut(s) 1948
Cfr10I RCCGGY 2 cut(s) 900, 2303
Cfr13I GGNCC 7 cut(s) 576, 735, 886, 1233, 1283, 1315, 1499
CseI GACGC 2 cut(s) 144, 1209
Csp6I GTAC 4 cut(s) 1011, 1763, 2623, 2677
CspAI ACCGGT 1 cut(s) 900
CviAII CATG 8 cut(s) 19, 2023, 2060, 2330, 2569, 2680, 2710, 2854
CviQI GTAC 4 cut(s) 1011, 1763, 2623, 2677
DinI GGCGCC 1 cut(s) 1947
DrdI GACNNNNNNGTC 1 cut(s) 1497
DseDI GACNNNNNNGTC 1 cut(s) 1497
EaeI YGGCCR 3 cut(s) 271, 675, 2138
EagI CGGCCG 1 cut(s) 271
Eam1104I CTCTTC 5 cut(s) 41, 303, 414, 1786, 2781
EarI CTCTTC 5 cut(s) 41, 303, 414, 1786, 2781
Ecl136II GAGCTC 1 cut(s) 235
EclXI CGGCCG 1 cut(s) 271
Eco130I CCWWGG 1 cut(s) 2767
Eco147I AGGCCT 2 cut(s) 279, 1575
Eco24I GRGCYC 1 cut(s) 237
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 6 cut(s) 576, 735, 886, 1283, 1315, 1499
Eco52I CGGCCG 1 cut(s) 271
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 4 cut(s) 438, 1151, 1963, 2880
Eco81I CCTNAGG 1 cut(s) 1365
Eco88I CYCGRG 3 cut(s) 211, 567, 763
Eco91I GGTNACC 1 cut(s) 767
EcoICRI GAGCTC 1 cut(s) 235
EcoNI CCTNNNNNAGG 1 cut(s) 2898
EcoO109I RGGNCCY 2 cut(s) 1233, 1315
EcoO65I GGTNACC 1 cut(s) 767
EcoRI GAATTC 3 cut(s) 754, 1042, 2192
EcoRII CCWGG 6 cut(s) 158, 597, 677, 873, 1638, 1738
EcoRV GATATC 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 2767
EcoT38I GRGCYC 1 cut(s) 237
EgeI GGCGCC 1 cut(s) 1947
EheI GGCGCC 1 cut(s) 1947
ErhI CCWWGG 1 cut(s) 2767
Esp3I CGTCTC 2 cut(s) 90, 1696
FaeI CATG 8 cut(s) 22, 2026, 2063, 2333, 2572, 2683, 2713, 2857
FalI AAGNNNNNCTT 2 cut(s) 1086, 1118
FatI CATG 8 cut(s) 18, 2022, 2059, 2329, 2568, 2679, 2709, 2853
FauI CCCGC 3 cut(s) 1045, 1075, 2090
FblI GTMKAC 2 cut(s) 171, 2112
FokI GGATG 3 cut(s) 719, 1346, 1985
FriOI GRGCYC 1 cut(s) 237
FspBI CTAG 7 cut(s) 518, 894, 1028, 2169, 2501, 2645, 2810
GlaI GCGC 1 cut(s) 1947
GsaI CCCAGC 1 cut(s) 2783
GsuI CTGGAG 3 cut(s) 181, 896, 1622
HaeII RGCGCY 1 cut(s) 1949
HaeIII GGCC 9 cut(s) 125, 273, 279, 677, 798, 1235, 1575, 2140, 2904
HgaI GACGC 2 cut(s) 144, 1209
HhaI GCGC 1 cut(s) 1948
Hin1I GRCGYC 1 cut(s) 1946
Hin1II CATG 8 cut(s) 22, 2026, 2063, 2333, 2572, 2683, 2713, 2857
Hin6I GCGC 1 cut(s) 1946
HinP1I GCGC 1 cut(s) 1946
HincII GTYRAC 3 cut(s) 172, 529, 2869
HindII GTYRAC 3 cut(s) 172, 529, 2869
HindIII AAGCTT 2 cut(s) 337, 2015
HpaI GTTAAC 1 cut(s) 529
Hpy99I CGWCG 5 cut(s) 47, 50, 98, 101, 248
HpyCH4III ACNGT 6 cut(s) 9, 1477, 1906, 2081, 2164, 2596
HpyCH4IV ACGT 1 cut(s) 99
HpySE526I ACGT 1 cut(s) 99
Hsp92I GRCGYC 1 cut(s) 1946
Hsp92II CATG 8 cut(s) 22, 2026, 2063, 2333, 2572, 2683, 2713, 2857
HspAI GCGC 1 cut(s) 1946
KasI GGCGCC 1 cut(s) 1945
Kpn2I TCCGGA 3 cut(s) 490, 703, 847
KspAI GTTAAC 1 cut(s) 529
LguI GCTCTTC 1 cut(s) 303
LmnI GCTCC 8 cut(s) 36, 61, 162, 984, 2105, 2720, 2769, 2878
LweI GCATC 4 cut(s) 1368, 2332, 2422, 2746
MaeI CTAG 7 cut(s) 518, 894, 1028, 2169, 2501, 2645, 2810
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 5 cut(s) 263, 767, 1268, 2653, 2705
MfeI CAATTG 3 cut(s) 187, 287, 1443
MflI RGATCY 5 cut(s) 71, 136, 215, 593, 2547
MhlI GDGCHC 5 cut(s) 237, 2059, 2560, 2703, 2725
MlsI TGGCCA 1 cut(s) 677
MluNI TGGCCA 1 cut(s) 677
Mly113I GGCGCC 1 cut(s) 1946
MlyI GAGTC 5 cut(s) 384, 523, 1752, 1781, 2260
MmeI TCCRAC 4 cut(s) 243, 1248, 1460, 1809
Mox20I TGGCCA 1 cut(s) 677
MroI TCCGGA 3 cut(s) 490, 703, 847
MroXI GAANNNNTTC 3 cut(s) 398, 721, 758
MscI TGGCCA 1 cut(s) 677
MseI TTAA 9 cut(s) 120, 341, 528, 669, 1197, 1238, 1472, 1725, 1911
MslI CAYNNNNRTG 2 cut(s) 1904, 2328
Msp20I TGGCCA 1 cut(s) 677
MspA1I CMGCKG 4 cut(s) 28, 524, 1154, 1871
MspCI CTTAAG 2 cut(s) 119, 1237
MunI CAATTG 3 cut(s) 187, 287, 1443
Mva1269I GAATGC 1 cut(s) 2753
MvaI CCWGG 6 cut(s) 160, 599, 679, 875, 1640, 1740
MvnI CGCG 1 cut(s) 68
NarI GGCGCC 1 cut(s) 1946
NciI CCSGG 4 cut(s) 224, 481, 694, 2659
NlaIII CATG 8 cut(s) 22, 2026, 2063, 2333, 2572, 2683, 2713, 2857
NlaIV GGNNCC 8 cut(s) 57, 561, 846, 1234, 1660, 1947, 2574, 2765
NmeAIII GCCGAG 3 cut(s) 299, 2541, 2740
NmuCI GTSAC 3 cut(s) 263, 2653, 2705
NruI TCGCGA 1 cut(s) 68
NspI RCATGY 1 cut(s) 2063
NspV TTCGAA 2 cut(s) 317, 2196
PagI TCATGA 1 cut(s) 2329
PaqCI CACCTGC 1 cut(s) 2330
PceI AGGCCT 2 cut(s) 279, 1575
PciSI GCTCTTC 1 cut(s) 303
PcsI WCGNNNNNNNCGW 2 cut(s) 45, 1335
PctI GAATGC 1 cut(s) 2753
PdmI GAANNNNTTC 3 cut(s) 398, 721, 758
PfeI GAWTC 5 cut(s) 682, 814, 1171, 1342, 1830
PflMI CCANNNNNTGG 1 cut(s) 1183
PfoI TCCNGGA 2 cut(s) 158, 479
PinAI ACCGGT 1 cut(s) 900
PleI GAGTC 5 cut(s) 383, 522, 1751, 1781, 2260
PluTI GGCGCC 1 cut(s) 1949
PpsI GAGTC 5 cut(s) 383, 522, 1751, 1781, 2260
PpuMI RGGWCCY 1 cut(s) 1315
PshAI GACNNNNGTC 2 cut(s) 177, 2496
Psp124BI GAGCTC 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 1315
Psp6I CCWGG 6 cut(s) 158, 597, 677, 873, 1638, 1738
PspEI GGTNACC 1 cut(s) 767
PspFI CCCAGC 1 cut(s) 2779
PspGI CCWGG 6 cut(s) 158, 597, 677, 873, 1638, 1738
PspN4I GGNNCC 8 cut(s) 57, 561, 846, 1234, 1660, 1947, 2574, 2765
PspPI GGNCC 7 cut(s) 576, 735, 886, 1233, 1283, 1315, 1499
PspPPI RGGWCCY 1 cut(s) 1315
PstI CTGCAG 3 cut(s) 1513, 1723, 1870
PstNI CAGNNNCTG 1 cut(s) 1874
PsuI RGATCY 5 cut(s) 71, 136, 215, 593, 2547
PvuII CAGCTG 3 cut(s) 524, 1154, 1871
RruI TCGCGA 1 cut(s) 68
RsaI GTAC 4 cut(s) 1012, 1764, 2624, 2678
RsaNI GTAC 4 cut(s) 1011, 1763, 2623, 2677
RseI CAYNNNNRTG 2 cut(s) 1904, 2328
SacI GAGCTC 1 cut(s) 237
SalI GTCGAC 1 cut(s) 170
SapI GCTCTTC 1 cut(s) 303
SaqAI TTAA 9 cut(s) 120, 341, 528, 669, 1197, 1238, 1472, 1725, 1911
Sau96I GGNCC 7 cut(s) 576, 735, 886, 1233, 1283, 1315, 1499
SchI GAGTC 5 cut(s) 384, 523, 1752, 1781, 2260
SduI GDGCHC 5 cut(s) 237, 2059, 2560, 2703, 2725
SfaNI GCATC 4 cut(s) 1368, 2332, 2422, 2746
SfcI CTRYAG 3 cut(s) 1509, 1719, 1866
SfoI GGCGCC 1 cut(s) 1947
SfuI TTCGAA 2 cut(s) 317, 2196
SinI GGWCC 6 cut(s) 576, 735, 886, 1283, 1315, 1499
SmiMI CAYNNNNRTG 2 cut(s) 1904, 2328
SmlI CTYRAG 7 cut(s) 119, 580, 730, 938, 1237, 1333, 2184
SmoI CTYRAG 7 cut(s) 119, 580, 730, 938, 1237, 1333, 2184
SseBI AGGCCT 2 cut(s) 279, 1575
SsiI CCGC 5 cut(s) 26, 1052, 1082, 1122, 2097
SspDI GGCGCC 1 cut(s) 1945
SspMI CTAG 7 cut(s) 518, 894, 1028, 2169, 2501, 2645, 2810
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 2 cut(s) 279, 1575
StyI CCWWGG 1 cut(s) 2767
TaaI ACNGT 6 cut(s) 9, 1477, 1906, 2081, 2164, 2596
TaiI ACGT 1 cut(s) 102
TaqII GACCGA 1 cut(s) 2541
TatI WGTACW 2 cut(s) 1762, 2676
TfiI GAWTC 5 cut(s) 682, 814, 1171, 1342, 1830
Tru1I TTAA 9 cut(s) 120, 341, 528, 669, 1197, 1238, 1472, 1725, 1911
Tru9I TTAA 9 cut(s) 120, 341, 528, 669, 1197, 1238, 1472, 1725, 1911
TscAI CASTG 4 cut(s) 985, 2056, 2167, 2730
TseFI GTSAC 3 cut(s) 263, 2653, 2705
Tsp45I GTSAC 3 cut(s) 263, 2653, 2705
TspDTI ATGAA 7 cut(s) 35, 881, 1107, 2318, 2349, 2435, 2819
TspGWI ACGGA 2 cut(s) 1678, 1907
TspRI CASTG 4 cut(s) 985, 2056, 2167, 2730
Van91I CCANNNNNTGG 1 cut(s) 1183
Vha464I CTTAAG 2 cut(s) 119, 1237
VneI GTGCAC 2 cut(s) 2055, 2556
VpaK11BI GGWCC 6 cut(s) 576, 735, 886, 1283, 1315, 1499
XagI CCTNNNNNAGG 1 cut(s) 2898
XapI RAATTY 8 cut(s) 486, 630, 754, 958, 1042, 1431, 2192, 2772
XbaI TCTAGA 2 cut(s) 1027, 2644
XceI RCATGY 1 cut(s) 2063
XcmI CCANNNNNNNNNTGG 1 cut(s) 570
XmiI GTMKAC 2 cut(s) 171, 2112
XmnI GAANNNNTTC 3 cut(s) 398, 721, 758
XspI CTAG 7 cut(s) 518, 894, 1028, 2169, 2501, 2645, 2810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.