pycom07g24400

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0011369
Physical Location & Seq
Forward (+)
25130263 .. 25134473
4211 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1875 bp
ATGGAAGAGATTCGGCTCGTTTTAAGGGGATCCTCATCCTTGGATCAGGTGAGGATCCTGATCCCTCTTCTCCACTGTCTGTCAAAAACCAGCACGCTCCTGCCCGACAAAGTCAAACCCCCAAATCCAAAAATCCTCCATTTTCTTATCCCATTTGTCATTCTTCGACCTCACTCCCTTTTTCTGTCATATTTAGGCTGTAAGCCTTCCACGACGGTGGACCTAAATTATCCTTTTCTTTTTCAATTTATTTTCCAAATTCCACAAGCTTTCGTATTTATAGGTAGAGACAGAGAAAGCGGGACGGGGGTAGAGAGAGAGGGAGAGAGGAAGGAAATGGGCAATTCATTCGGTTGCTCCGCCTCCGGCGAACGGCTGGTTTCGGCGGCGAGAGACGGGGATGTAATCGAGGCCAAAATGCTCTTGGATTGCAATCCCTGCCTCTCCAAATACTCCACCTTCGGCGGCCTTAACTCCCCTCTCCATTTCGCCGCCGCTAAAGGCCACAACGAGATTGTTTCTTTGCTGCTGGAGAATGGAGCTGATGCCAATTCCAGGAATTACTGCGGGCAGACAGCATTGATGCAAGCATGCCGATATGGACATTGGGAAGTTGTACAGACCCTCTTGCTCTTCAGATCCAATGTCACTAGAGCAGACTATCTTACTGGTAGGACTGCTCTTCATTTTGCTGCTATAAATGGGCATGTAAGATGTATAAGACTTGTCGTGGCTGACTTTGTTCCAAGTGCTCCCTATGAATCTCTAACTGCTCAGACAGAGTGCAACAGAGGTGATAGTGCAAAGGTCGTGAACAAAAATGAGCAAAGTGCTCTATCCAAACTTGTAAATAAGGTAGCAGATTGTGGTATTACTGCTCTCCATATGGCTGCATTGAATGGATCTTTTGGTTGTGTACAACTCCTAATCGATCTTCAAGCAAATGTGTCTGCTGTGACGTTTCATTACGGGACTTCTATGGATTTGATAGGTGCTGGAAGTTCTCCACTGCATTATGCTGCTTGTGGGGGTAACGTAAAGTGCTGTCAGATCCTCCTTGCAAGAGGTGCCAGCAAGATGACTTTAAACTGCAATGGGTGGCTTCCAGTTGATGTTGCCCGAATGTGGGGGCGCCGCTGGCTTGAACCACTTCTAGCACCCAATTCTGACTCATCAATACCAGCGTTCCCCCTCTCAAGTTACCTATCTTTGCCTCTCATGAGCATAATGAACATAGCAAGGGAATGTGGATTTAAGTCTGTGACAACATCAGTGGACGACGCAGATACTTGTGCTGTCTGTCTGGAAAGACCATGTTTGGTGGCGGCAGAAGGCTGTAAGCATGAGCTTTGTGTAAGATGCGCACTATATCTTTGCTCAACAAGCAACATACCTTCGGAAACGGTGGGCCCACCTGGGTCCATTCCGTGCCCATTTTGTAGGCATGGAATCATCTCCTTTGCCAAATTGCCTGGCTCCCCGGCAAAGGAAAATAGACTACAGATGTCACTTGGCCTTTGTACCCCTTGTATGCTTCATCCTCGTGACCCAGAGCGTCTATCTCCAGCTTGTGCACCAGAGATCCGAAAGAACCGTGTGGCTTCAGTTTCTTCAGATATGCTCTGTGCAGTCACCTGCAGTCCGTTTCCTTCTGTCACCATTCCTTTGTGCACCTGCAATGATGGTCCATGCCCACCATTTGAATCCCGGGAGGTAGAAACCGGAGATGAATTGCCCAGACATCCCCAAGCAACATCAACAGACCAGGATAAGATGGAAGGGCCGAGACTTGGAAAAACAACATGTTCAAGCATGTTTTGGAGCAGAAGAAGCTGCACCAGGGAGCATCAGTGCAATGCTGAAATAAATGCTTAA

Protein Analysis

625

Amino Acids

67.86

Weight (kDa)

7.81

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 128 - 210 4.9e-14 Ankyrin repeats (3 copies)
Ank PF00023 158 - 187 1.3e-07 Ankyrin repeat
Ank_4 PF13637 161 - 210 1.7e-12 Ankyrin repeats (many copies)
Ank_2 PF12796 162 - 244 2.5e-17 Ankyrin repeats (3 copies)
Ank_5 PF13857 176 - 215 3.7e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 219 - 317 4.6e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 277 - 359 1.7e-11 Ankyrin repeats (3 copies)
RING_XB3-XBAT31 PF24921 430 - 485 2.6e-17 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1643, 1682
Acc16I TGCGCA 1 cut(s) 1363
Acc36I ACCTGC 2 cut(s) 1643, 1682
AccB1I GGYRCC 2 cut(s) 1067, 1131
AciI CCGC 9 cut(s) 300, 360, 386, 465, 492, 495, 567, 1135, 1325
AcsI RAATTY 1 cut(s) 258
AcuI CTGAAG 3 cut(s) 619, 1587, 1596
AcyI GRCGYC 1 cut(s) 1132
AfaI GTAC 3 cut(s) 618, 918, 1522
AfiI CCNNNNNNNGG 6 cut(s) 372, 555, 1125, 1126, 1486, 1790
AflIII ACRYGT 1 cut(s) 1802
AgsI TTSAA 6 cut(s) 245, 898, 938, 1145, 1703, 1809
AjnI CCWGG 5 cut(s) 554, 1414, 1471, 1764, 1838
AleI CACNNNNGTG 1 cut(s) 215
AluBI AGCT 5 cut(s) 269, 542, 1348, 1568, 1833
AluI AGCT 5 cut(s) 269, 542, 1348, 1568, 1833
Alw21I GWGCWC 4 cut(s) 754, 835, 1576, 1673
Alw26I GTCTC 3 cut(s) 282, 387, 1780
Alw44I GTGCAC 2 cut(s) 1572, 1669
Ama87I CYCGRG 1 cut(s) 1707
AoxI GGCC 6 cut(s) 411, 466, 502, 1408, 1513, 1781
ApaI GGGCCC 1 cut(s) 1412
ApaLI GTGCAC 2 cut(s) 1572, 1669
ApeKI GCWGC 5 cut(s) 526, 692, 890, 1019, 1833
ApoI RAATTY 1 cut(s) 258
Asp700I GAANNNNTTC 2 cut(s) 9, 1149
AspLEI GCGC 2 cut(s) 1134, 1364
AspS9I GGNCC 6 cut(s) 220, 1408, 1409, 1419, 1685, 1781
AsuC2I CCSGG 3 cut(s) 1481, 1708, 1709
AsuHPI GGTGA 4 cut(s) 61, 806, 1624, 1648
AvaI CYCGRG 1 cut(s) 1707
AvaII GGWCC 3 cut(s) 220, 1419, 1685
BaeGI GKGCMC 4 cut(s) 1412, 1433, 1576, 1673
BamHI GGATCC 2 cut(s) 29, 54
BanI GGYRCC 2 cut(s) 1067, 1131
BanII GRGCYC 1 cut(s) 1412
BauI CACGAG 1 cut(s) 1542
Bbv12I GWGCWC 4 cut(s) 754, 835, 1576, 1673
BbvI GCAGC 5 cut(s) 513, 679, 877, 1006, 1820
BccI CCATC 2 cut(s) 1676, 1768
BceAI ACGGC 1 cut(s) 389
BcgI CGANNNNNNTGC 2 cut(s) 331, 365
BciT130I CCWGG 5 cut(s) 556, 1416, 1473, 1766, 1840
BcnI CCSGG 3 cut(s) 1481, 1708, 1709
BcoDI GTCTC 3 cut(s) 282, 387, 1780
BfaI CTAG 2 cut(s) 651, 1154
BfmI CTRYAG 2 cut(s) 1499, 1636
BfoI RGCGCY 1 cut(s) 1135
BfuAI ACCTGC 2 cut(s) 1643, 1682
Bme1390I CCNGG 8 cut(s) 556, 1416, 1473, 1481, 1708, 1709, 1766, 1840
Bme18I GGWCC 3 cut(s) 220, 1419, 1685
BmeT110I CYCGRG 1 cut(s) 1707
BmgT120I GGNCC 6 cut(s) 220, 1408, 1409, 1419, 1685, 1781
BmiI GGNNCC 7 cut(s) 31, 56, 1069, 1133, 1410, 1420, 1477
BmrFI CCNGG 8 cut(s) 556, 1416, 1473, 1481, 1708, 1709, 1766, 1840
BmsI GCATC 4 cut(s) 535, 573, 1349, 1855
BpmI CTGGAG 2 cut(s) 551, 1548
BpuEI CTTGAG 1 cut(s) 1180
BpuMI CCSGG 3 cut(s) 1481, 1708, 1709
Bsa29I ATCGAT 1 cut(s) 930
BsaBI GATNNNNATC 3 cut(s) 34, 59, 432
BsaHI GRCGYC 1 cut(s) 1132
BsaJI CCNNGG 5 cut(s) 39, 1415, 1479, 1707, 1839
BsaWI WCCGGW 1 cut(s) 1721
BsaXI ACNNNNNCTCC 2 cut(s) 1835, 1865
Bsc4I CCNNNNNNNGG 6 cut(s) 372, 555, 1125, 1126, 1486, 1790
Bse1I ACTGG 2 cut(s) 673, 1106
Bse3DI GCAATG 3 cut(s) 1099, 1684, 1861
Bse8I GATNNNNATC 3 cut(s) 34, 59, 432
BseBI CCWGG 5 cut(s) 556, 1416, 1473, 1766, 1840
BseCI ATCGAT 1 cut(s) 930
BseDI CCNNGG 5 cut(s) 39, 1415, 1479, 1707, 1839
BseGI GGATG 4 cut(s) 35, 406, 1537, 1741
BseJI GATNNNNATC 3 cut(s) 34, 59, 432
BseLI CCNNNNNNNGG 6 cut(s) 372, 555, 1125, 1126, 1486, 1790
BseMI GCAATG 3 cut(s) 1099, 1684, 1861
BseMII CTCAG 1 cut(s) 788
BseNI ACTGG 2 cut(s) 673, 1106
BseSI GKGCMC 4 cut(s) 1412, 1433, 1576, 1673
BseXI GCAGC 5 cut(s) 513, 679, 877, 1006, 1820
BsgI GTGCAG 2 cut(s) 1647, 1819
BshFI GGCC 6 cut(s) 413, 468, 504, 1410, 1515, 1783
BshNI GGYRCC 2 cut(s) 1067, 1131
BshVI ATCGAT 1 cut(s) 930
BsiHKAI GWGCWC 4 cut(s) 754, 835, 1576, 1673
BsiHKCI CYCGRG 1 cut(s) 1707
BsiSI CCGG 4 cut(s) 366, 1481, 1708, 1722
BslFI GGGAC 2 cut(s) 316, 985
BslI CCNNNNNNNGG 6 cut(s) 372, 555, 1125, 1126, 1486, 1790
BsmAI GTCTC 3 cut(s) 282, 387, 1780
BsmBI CGTCTC 1 cut(s) 387
BsmFI GGGAC 2 cut(s) 316, 985
BsnI GGCC 6 cut(s) 413, 468, 504, 1410, 1515, 1783
BsoBI CYCGRG 1 cut(s) 1707
Bsp120I GGGCCC 1 cut(s) 1408
Bsp1286I GDGCHC 6 cut(s) 754, 835, 1412, 1433, 1576, 1673
Bsp1407I TGTACA 2 cut(s) 616, 916
Bsp143I GATC 9 cut(s) 29, 43, 54, 60, 638, 902, 931, 1050, 1581
BspACI CCGC 9 cut(s) 300, 360, 386, 465, 492, 495, 567, 1135, 1325
BspANI GGCC 6 cut(s) 413, 468, 504, 1410, 1515, 1783
BspCNI CTCAG 1 cut(s) 787
BspDI ATCGAT 1 cut(s) 930
BspHI TCATGA 1 cut(s) 1218
BspLI GGNNCC 7 cut(s) 31, 56, 1069, 1133, 1410, 1420, 1477
BspMAI CTGCAG 1 cut(s) 1640
BspMI ACCTGC 2 cut(s) 1643, 1682
BspQI GCTCTTC 2 cut(s) 638, 687
BspT107I GGYRCC 2 cut(s) 1067, 1131
BsrDI GCAATG 3 cut(s) 1099, 1684, 1861
BsrGI TGTACA 2 cut(s) 616, 916
BsrI ACTGG 2 cut(s) 673, 1106
BssECI CCNNGG 5 cut(s) 39, 1415, 1479, 1707, 1839
BssMI GATC 9 cut(s) 29, 43, 54, 60, 638, 902, 931, 1050, 1581
BssNI GRCGYC 1 cut(s) 1132
BssSI CACGAG 1 cut(s) 1542
BssT1I CCWWGG 1 cut(s) 39
Bst2BI CACGAG 1 cut(s) 1542
Bst2UI CCWGG 5 cut(s) 556, 1416, 1473, 1766, 1840
Bst4CI ACNGT 4 cut(s) 77, 217, 1405, 1595
Bst6I CTCTTC 3 cut(s) 72, 638, 687
BstACI GRCGYC 1 cut(s) 1132
BstAPI GCANNNNNTGC 2 cut(s) 438, 1067
BstAUI TGTACA 2 cut(s) 616, 916
BstC8I GCNNGC 6 cut(s) 95, 569, 588, 592, 1072, 1139
BstDEI CTNAG 1 cut(s) 774
BstF5I GGATG 4 cut(s) 35, 406, 1537, 1741
BstH2I RGCGCY 1 cut(s) 1135
BstHHI GCGC 2 cut(s) 1134, 1364
BstKTI GATC 9 cut(s) 32, 46, 57, 63, 641, 905, 934, 1053, 1584
BstMAI GTCTC 3 cut(s) 282, 387, 1780
BstMBI GATC 9 cut(s) 29, 43, 54, 60, 638, 902, 931, 1050, 1581
BstMWI GCNNNNNNNGC 5 cut(s) 438, 1067, 1138, 1383, 1830
BstNI CCWGG 5 cut(s) 556, 1416, 1473, 1766, 1840
BstNSI RCATGY 4 cut(s) 594, 710, 1806, 1816
BstSCI CCNGG 8 cut(s) 554, 1414, 1471, 1479, 1706, 1707, 1764, 1838
BstSFI CTRYAG 2 cut(s) 1499, 1636
BstSLI GKGCMC 4 cut(s) 1412, 1433, 1576, 1673
BstV1I GCAGC 5 cut(s) 513, 679, 877, 1006, 1820
BstX2I RGATCY 6 cut(s) 29, 54, 638, 902, 1050, 1581
BstXI CCANNNNNNTGG 1 cut(s) 217
BstYI RGATCY 6 cut(s) 29, 54, 638, 902, 1050, 1581
Bsu15I ATCGAT 1 cut(s) 930
BsuRI GGCC 6 cut(s) 413, 468, 504, 1410, 1515, 1783
BsuTUI ATCGAT 1 cut(s) 930
BtsCI GGATG 4 cut(s) 35, 406, 1537, 1741
BtsI GCAGTG 1 cut(s) 1007
BtsIMutI CAGTG 4 cut(s) 73, 1007, 1278, 1856
BveI ACCTGC 2 cut(s) 1643, 1682
Cac8I GCNNGC 6 cut(s) 95, 569, 588, 592, 1072, 1139
CciI TCATGA 1 cut(s) 1218
CfoI GCGC 2 cut(s) 1134, 1364
Cfr13I GGNCC 6 cut(s) 220, 1408, 1409, 1419, 1685, 1781
Cfr9I CCCGGG 1 cut(s) 1707
ClaI ATCGAT 1 cut(s) 930
CseI GACGC 2 cut(s) 1289, 1544
Csp6I GTAC 3 cut(s) 617, 917, 1521
CspCI CAANNNNNGTGG 2 cut(s) 1254, 1289
CviAII CATG 9 cut(s) 591, 707, 1219, 1314, 1343, 1445, 1689, 1803, 1813
CviQI GTAC 3 cut(s) 617, 917, 1521
DdeI CTNAG 1 cut(s) 774
DinI GGCGCC 1 cut(s) 1133
DpnI GATC 9 cut(s) 31, 45, 56, 62, 640, 904, 933, 1052, 1583
DpnII GATC 9 cut(s) 29, 43, 54, 60, 638, 902, 931, 1050, 1581
DraI TTTAAA 1 cut(s) 1086
Eam1104I CTCTTC 3 cut(s) 72, 638, 687
EarI CTCTTC 3 cut(s) 72, 638, 687
EciI GGCGGA 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 1412
Eco47I GGWCC 3 cut(s) 220, 1419, 1685
Eco57I CTGAAG 3 cut(s) 619, 1587, 1596
Eco88I CYCGRG 1 cut(s) 1707
EcoRII CCWGG 5 cut(s) 554, 1414, 1471, 1764, 1838
EcoT14I CCWWGG 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 1412
EgeI GGCGCC 1 cut(s) 1133
EheI GGCGCC 1 cut(s) 1133
ErhI CCWWGG 1 cut(s) 39
Esp3I CGTCTC 1 cut(s) 387
FaeI CATG 9 cut(s) 594, 710, 1222, 1317, 1346, 1448, 1692, 1806, 1816
FaqI GGGAC 2 cut(s) 316, 985
FatI CATG 9 cut(s) 590, 706, 1218, 1313, 1342, 1444, 1688, 1802, 1812
FauI CCCGC 2 cut(s) 293, 560
FauNDI CATATG 1 cut(s) 885
FokI GGATG 4 cut(s) 22, 413, 1524, 1728
FriOI GRGCYC 1 cut(s) 1412
FspAI RTGCGCAY 1 cut(s) 1363
FspBI CTAG 2 cut(s) 651, 1154
FspI TGCGCA 1 cut(s) 1363
GlaI GCGC 2 cut(s) 1133, 1363
GsuI CTGGAG 2 cut(s) 551, 1548
HaeII RGCGCY 1 cut(s) 1135
HaeIII GGCC 6 cut(s) 413, 468, 504, 1410, 1515, 1783
HapII CCGG 4 cut(s) 366, 1481, 1708, 1722
HgaI GACGC 2 cut(s) 1289, 1544
HhaI GCGC 2 cut(s) 1134, 1364
Hin1I GRCGYC 1 cut(s) 1132
Hin1II CATG 9 cut(s) 594, 710, 1222, 1317, 1346, 1448, 1692, 1806, 1816
Hin6I GCGC 2 cut(s) 1132, 1362
HinP1I GCGC 2 cut(s) 1132, 1362
HindIII AAGCTT 1 cut(s) 267
HinfI GANTC 5 cut(s) 10, 761, 1169, 1449, 1703
HpaII CCGG 4 cut(s) 366, 1481, 1708, 1722
HphI GGTGA 4 cut(s) 61, 806, 1624, 1648
Hpy166II GTNNAC 6 cut(s) 220, 814, 917, 1276, 1574, 1671
Hpy188I TCNGA 7 cut(s) 638, 777, 1050, 1168, 1399, 1586, 1615
Hpy188III TCNNGA 5 cut(s) 58, 811, 1219, 1304, 1544
Hpy8I GTNNAC 6 cut(s) 220, 814, 917, 1276, 1574, 1671
Hpy99I CGWCG 2 cut(s) 217, 1283
HpyAV CCTTC 7 cut(s) 216, 325, 469, 1325, 1404, 1659, 1772
HpyCH4III ACNGT 4 cut(s) 77, 217, 1405, 1595
HpyCH4IV ACGT 2 cut(s) 959, 1035
HpyF10VI GCNNNNNNNGC 5 cut(s) 438, 1067, 1138, 1383, 1830
HpyF3I CTNAG 1 cut(s) 774
HpySE526I ACGT 2 cut(s) 959, 1035
Hsp92I GRCGYC 1 cut(s) 1132
Hsp92II CATG 9 cut(s) 594, 710, 1222, 1317, 1346, 1448, 1692, 1806, 1816
HspAI GCGC 2 cut(s) 1132, 1362
KasI GGCGCC 1 cut(s) 1131
Kzo9I GATC 9 cut(s) 29, 43, 54, 60, 638, 902, 931, 1050, 1581
LguI GCTCTTC 2 cut(s) 638, 687
LmnI GCTCC 7 cut(s) 102, 362, 539, 757, 1481, 1821, 1843
Lsp1109I GCAGC 5 cut(s) 513, 679, 877, 1006, 1820
LweI GCATC 4 cut(s) 535, 573, 1349, 1855
MaeI CTAG 2 cut(s) 651, 1154
MaeII ACGT 2 cut(s) 959, 1035
MaeIII GTNAC 9 cut(s) 646, 955, 1031, 1199, 1261, 1506, 1544, 1630, 1654
MalI GATC 9 cut(s) 31, 45, 56, 62, 640, 904, 933, 1052, 1583
MboI GATC 9 cut(s) 29, 43, 54, 60, 638, 902, 931, 1050, 1581
MboII GAAGA 8 cut(s) 17, 59, 155, 625, 674, 926, 1602, 1839
MflI RGATCY 6 cut(s) 29, 54, 638, 902, 1050, 1581
MhlI GDGCHC 6 cut(s) 754, 835, 1412, 1433, 1576, 1673
MluCI AATT 9 cut(s) 226, 245, 258, 343, 550, 559, 1162, 1466, 1730
Mly113I GGCGCC 1 cut(s) 1132
MlyI GAGTC 1 cut(s) 1163
MroXI GAANNNNTTC 2 cut(s) 9, 1149
MseI TTAA 5 cut(s) 23, 471, 1085, 1254, 1873
MslI CAYNNNNRTG 2 cut(s) 215, 1542
MspA1I CMGCKG 1 cut(s) 1137
MspI CCGG 4 cut(s) 366, 1481, 1708, 1722
MspR9I CCNGG 8 cut(s) 556, 1416, 1473, 1481, 1708, 1709, 1766, 1840
MvaI CCWGG 5 cut(s) 556, 1416, 1473, 1766, 1840
MwoI GCNNNNNNNGC 5 cut(s) 438, 1067, 1138, 1383, 1830
NarI GGCGCC 1 cut(s) 1132
NciI CCSGG 3 cut(s) 1481, 1708, 1709
NdeI CATATG 1 cut(s) 885
NdeII GATC 9 cut(s) 29, 43, 54, 60, 638, 902, 931, 1050, 1581
NlaIII CATG 9 cut(s) 594, 710, 1222, 1317, 1346, 1448, 1692, 1806, 1816
NlaIV GGNNCC 7 cut(s) 31, 56, 1069, 1133, 1410, 1420, 1477
NmeAIII GCCGAG 1 cut(s) 1809
NmuCI GTSAC 7 cut(s) 646, 955, 1261, 1506, 1544, 1630, 1654
NsbI TGCGCA 1 cut(s) 1363
NspI RCATGY 4 cut(s) 594, 710, 1806, 1816
OliI CACNNNNGTG 1 cut(s) 215
PaeI GCATGC 1 cut(s) 594
PagI TCATGA 1 cut(s) 1218
PaqCI CACCTGC 2 cut(s) 1643, 1682
PciI ACATGT 1 cut(s) 1802
PciSI GCTCTTC 2 cut(s) 638, 687
PdmI GAANNNNTTC 2 cut(s) 9, 1149
PfeI GAWTC 4 cut(s) 10, 761, 1449, 1703
PflFI GACNNNGTC 1 cut(s) 110
PfoI TCCNGGA 1 cut(s) 554
PleI GAGTC 1 cut(s) 1163
PluTI GGCGCC 1 cut(s) 1135
PpsI GAGTC 1 cut(s) 1163
PscI ACATGT 1 cut(s) 1802
Psp6I CCWGG 5 cut(s) 554, 1414, 1471, 1764, 1838
PspGI CCWGG 5 cut(s) 554, 1414, 1471, 1764, 1838
PspN4I GGNNCC 7 cut(s) 31, 56, 1069, 1133, 1410, 1420, 1477
PspOMI GGGCCC 1 cut(s) 1408
PspPI GGNCC 6 cut(s) 220, 1408, 1409, 1419, 1685, 1781
PstI CTGCAG 1 cut(s) 1640
PsuI RGATCY 6 cut(s) 29, 54, 638, 902, 1050, 1581
PsyI GACNNNGTC 1 cut(s) 110
RsaI GTAC 3 cut(s) 618, 918, 1522
RsaNI GTAC 3 cut(s) 617, 917, 1521
RseI CAYNNNNRTG 2 cut(s) 215, 1542
SapI GCTCTTC 2 cut(s) 638, 687
SaqAI TTAA 5 cut(s) 23, 471, 1085, 1254, 1873
Sau3AI GATC 9 cut(s) 29, 43, 54, 60, 638, 902, 931, 1050, 1581
Sau96I GGNCC 6 cut(s) 220, 1408, 1409, 1419, 1685, 1781
SchI GAGTC 1 cut(s) 1163
ScrFI CCNGG 8 cut(s) 556, 1416, 1473, 1481, 1708, 1709, 1766, 1840
SduI GDGCHC 6 cut(s) 754, 835, 1412, 1433, 1576, 1673
SfaNI GCATC 4 cut(s) 535, 573, 1349, 1855
SfcI CTRYAG 2 cut(s) 1499, 1636
SfoI GGCGCC 1 cut(s) 1133
SinI GGWCC 3 cut(s) 220, 1419, 1685
SmaI CCCGGG 1 cut(s) 1709
SmiMI CAYNNNNRTG 2 cut(s) 215, 1542
SmlI CTYRAG 1 cut(s) 1195
SmoI CTYRAG 1 cut(s) 1195
SphI GCATGC 1 cut(s) 594
Sse9I AATT 9 cut(s) 226, 245, 258, 343, 550, 559, 1162, 1466, 1730
SsiI CCGC 9 cut(s) 300, 360, 386, 465, 492, 495, 567, 1135, 1325
SspDI GGCGCC 1 cut(s) 1131
SspMI CTAG 2 cut(s) 651, 1154
StyD4I CCNGG 8 cut(s) 554, 1414, 1471, 1479, 1706, 1707, 1764, 1838
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 4 cut(s) 77, 217, 1405, 1595
TaiI ACGT 2 cut(s) 962, 1038
TaqI TCGA 3 cut(s) 166, 408, 930
TasI AATT 9 cut(s) 226, 245, 258, 343, 550, 559, 1162, 1466, 1730
TatI WGTACW 2 cut(s) 616, 916
TauI GCSGC 6 cut(s) 389, 468, 494, 497, 1137, 1328
TfiI GAWTC 4 cut(s) 10, 761, 1449, 1703
Tru1I TTAA 5 cut(s) 23, 471, 1085, 1254, 1873
Tru9I TTAA 5 cut(s) 23, 471, 1085, 1254, 1873
TscAI CASTG 4 cut(s) 80, 1014, 1278, 1856
TseFI GTSAC 7 cut(s) 646, 955, 1261, 1506, 1544, 1630, 1654
TseI GCWGC 5 cut(s) 526, 692, 890, 1019, 1833
Tsp45I GTSAC 7 cut(s) 646, 955, 1261, 1506, 1544, 1630, 1654
TspDTI ATGAA 7 cut(s) 336, 674, 774, 953, 1244, 1526, 1743
TspGWI ACGGA 2 cut(s) 1416, 1632
TspMI CCCGGG 1 cut(s) 1707
TspRI CASTG 4 cut(s) 80, 1014, 1278, 1856
Tth111I GACNNNGTC 1 cut(s) 110
VneI GTGCAC 2 cut(s) 1572, 1669
VpaK11BI GGWCC 3 cut(s) 220, 1419, 1685
XapI RAATTY 1 cut(s) 258
XceI RCATGY 4 cut(s) 594, 710, 1806, 1816
XcmI CCANNNNNNNNNTGG 1 cut(s) 421
XmaI CCCGGG 1 cut(s) 1707
XmnI GAANNNNTTC 2 cut(s) 9, 1149
XspI CTAG 2 cut(s) 651, 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.