Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0011400

Physical Location & Seq

Genomic Coordinates Reverse (-)

25414628 .. 25427305

Gene Structure 12678 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4125 bp

ATGCAAAACCCAGTGGTTCTGTTCAAATTGCCGCATTTGCCCTCGTCACGACCTTCATATCACTCGCCATTTTTCTCACTCATCATTAGACACAAAAAGCTAAGCAAAGCGAGAAGACCGAGAGAGAGAGAGTGGAGTGGCAGTGGCAATAGCAGGAGCAGAAGTTGCAGTGGTGGTGGGGTCTGGGCAATGCCTTGCTCTGCAATCGGCGGTGCTGGCGGAGGCGGTGGCGACGGTAATGGTGCTCTGACCAATTTCAAACTCAACGAGTCCACGTTCTTGGCCTCCCTCATGCCGAAGAAAGAAATTGGCGTCGATCGATTCATCGATGCGCATCCCAACTACGACGGCCGCGGCGCCCTCATCGCTATCTTCGATTCTGGAGTGGATCGATCTGCTTCTGGATTACAAGTTACATCTGATGGAAAGCCGAAAGTCCTAGATGTTCTTGATTGCACCGGGAGTGGCGATGTTGATACTTCGAAGGTAGTCAAGGCCGATGACAATGGTTGTATTCGTGGAGCTTCAGGAGCATCTCTGTTTGTTGACTCTTCATGGAAAAATCCTTCTGGTGAATGGCATGTGGGCTATAAGTTGGTATATGAGCTATTTACGGATACGTTGACTTCTCGCTTGAAGAAAGAAAGAAGGAAGAAATGGGATGAACAAAACCAAGAAGAAATTGCCAAGGCTGTTAAACATCTTCAAGAATTTGATCAGAAACACAGTAGAGTGGATGACGTTAACTTGAAAAGAGCTAGGGAGGACCTGCAAAATAGAGTTGATTACCTACAAAATCAAGCTGATAGCTATGATGACAAAGGGCCTGTCATAGATGCCGTTGTATGGCATGATGGAGAAGTATGGAGGGTTGCCCTTGACACACAGACTCTTGAGGATAACCCAGATTGTGGAAAACTTGCAAACTTTGTGCCCCTAACTAATTATAGGATAGAACGAAAATATGGTGTTTTTAGCAAATTAGATGCCTGTACATTTGTTGCTAATGTATACGATGAAGGGAATATCCTTAGTATCGTAACAGATAGCTCTCCACATGGCACTCATGTTGCTGGTATTGCTACTGCTTTCCACCCGAAGGAACCCCTGTTGAATGGAGTTGCACCTGGAGCACAAATAATATCTTGTAAGATTGGAGACTCGCGTTTAGGTTCAATGGAGACTGGAACTGGCTTGACTCGAGCCTTGATTGCTGCTGTGGAGCATAAATGTGATTTGATCAACATGAGTTATGGAGAACCTACACTGCTGCCAGACTATGGGCGTTTTGTTGACCTTGTTGATGAAGCTGTGAACAAGCACCATCTAATATTTGTGAGTAGTGCTGGCAATAGTGGGCCAGCATTGAGCACTGTTGGAGCACCTGGTGGTACCACATCAAGCATTATAGGAGTTGGTGCATATGTCTCTCCTGCAATGGCTGCTGGTGCTCATTGTGTAGTTGAAGCTCCAGGCGAAGGGCTTGAGTACACTTGGTCTAGTCGTGGGCCAACCAGTGATGGAGCTCTTGGTGTCTGTATAAGTGCTCCTGGTGCAGCTGTTGCTCCTGTTCCTACATGGACTCTTCAGCAACGCATGCTCATGAATGGAACATCAATGTCATCTCCATCTGCTTGTGGGGGAATTGCACTGCTCGTTAGTGCATTGAAGGCTGAGGATATTCCTGTGAGTCCATACAGTGTAAGGAAGGCTCTTGAAAATACATCTGTTCCCATAGGCAGTTTGCCTGAAGATAAACTATCAACTGGACAAGGGCTTATGCAAGTTGACAAGGCACATGAATATCTAAGGCAGTGCCGGGATGTACCATGTGTTTGGTATCAGATACAGATAAATCAGTCTGGTAAAACAACTCCTACATCGCGAGGAATCTACTTACGGGAGGCTAGTGCTTTCCAACAATCAACCGAGTGGACAGTGCAAGTTGAACCGAAATTTCATGAAGGTGCTAGCAATTTGGAAGATTTGGTTCCTTTTGAAGAGTGTATTGAGCTACATTCCAGTGACAAGGCAGTTTTGAGGGCACCTGATTTTCTCCTTCTTACTCATAATGGGCGTAGCTTCAACATAGTAGTGGATCCCACCAAGCTTAGTGAAGGTCTGCACTATTTCGAACTCTATGGTGTTGATTGTAAAGCACCATGGCGTGGCCCTCTTTTCAGAATCCCTGTTACCATAACAAAGCCTATAGCTGTGATAAGTCGACCTCCACTGTTGTCATTCTCTAGGATGTCATTTTTGCCAGGCCACATAGAAAGGAGATTTATAGAAGTACCTCTTGGTGCTACATGGGTTGAAGCAACTATGCAAACATCAGGATTTGATACAACAAGAAGATTTTTTGTTGACTCTGTTCAGCTATGTCCCCTGCAAAGGCCTCTTAAATGGGAGAGTGTTGTAACATTCTCTTCTCCTGCTGCCAAAAGCTTTTCATTTCCTGTTGTGGGTGGCCAAACAATGGAATTAGCTATTGCTCAATTTTGGTCGAGTGGCTTAGGAAGCCATGAAACAACTATTGTGGATTTTGAGATTGTATTTCATGGAATTAACATTAATAAAGATGAAGTAGTACTTGATGGAAGTGAAGGGCCGACCAGAATTGAAGCTGAAGCTCTACTGGCTTCTGAGACACTTGCACCGGTTGCTATTCTAAACAAGATAAGAATTCCATACCGGCCGGTTGAGTCTAAACTTTGTTCTCTCTCAACAGATCGTGACAAACTACCCTCAGAAAAACGTATTATGGCACTTACGTTAACCTACAAGATCAAATTGGAAGACGGAGCTGAAGTAAAGCCTCAAGTTCCGTTACTTAACAATCGCGTATATGACACCAAATTTGAGTCACAATTTTATATGATCTCTGATGCAAACAAGCGTATATATGCAATGGGAGACACCTATCCGAGTAAATCAAAACTGCCCAAGGGAGAGTACAATTTACGGCTATATCTAAGACATGACAATGTGCAATACTTGGAAAAACTGAAGCAGCTGGTTTTGTTCATCGAGAGAAATTTGGAAGAGAAGGATGTAATCCGATTGAGCTTCTTCTCCCAACCTGATGGCCCTTTGATGGGAAACGGTAGTTTTAAGTCCTCGGTATTAGTTCCAGGGAAAAAGGAAGCATTCTATTTAGGTCCACCATCCAAAGACAAGCTTCTAAAGTTTTCTCCACAAGGATCTGTATTACTTGGAGCCATCTCGTATGGGAAGCTATCATATGTTGATAAGGGAGAGCAGAAAAATCCGCTTAAGAACCCCGTGTCTTATCAAATTTCTTATATAGTGCCACCAAATAAGCTGGACGAGGAAAAAGGAAAAGGTTCTTCTACTTCCACCAAGCCTGTTTCTGAGCGGTTAGAAGAAGAGGTACGGGATGCAAAGATTAAAGTTCTTGCAAGCCTGAAGCAAGGTACTGAGGAAGAATGCTTGGAGTGGAATAAATTATCGTCTTCTCTCAAGTCTGAATATCCTAAATACACCCCATTGCTTGCCAAAATATTGGAAGCTGTACTTTCTCGGAACAATGACAAGGACAAAATCTCCCATGAAAAAGAGGTTATTGATGCAGCAAATGAGGTGGTTGACAGTGTTGACAGAGATGAGCTGGCAAAATTTTTTGCACTAAGAAGTGATCCTGATGATGAAGAGGCAGAGAAAATTAAAAAGAAGATGGAGACAACCCGTGATCGATTAGCAGAGGCATTGTACCAGAAAGGACTGGCACTAGCAGAGATTGAATCTTTGCAGGGTGATAAGCCTGCAGAGGCTGAAGGTGCAGAAGGGGAAGAGAAGATTGAAGATCTTTCACAACCAGCCCCAGATTTGTTTGAAAACAACTTTAAAGAACTTCAAAAATGGGTTGAAGTCAAGTCCTCCAAATACGGAACTCTCTCAGTACTACGTGAGAAACGTGCTGGAAGGCATGGAACCGCACTGAAGGTTCTGAATGACGTAATTCAAGACGATGGAGAGCTTCCCAAGAAGAAGTTCTACGACCTGAAGATTTCCTTGCTCGATGACATTGGTTGGCAACATTTGGCGACACATGAGAGGCAATGGATGCACGTTCGCTTTCCACCCAGTTTACCTCTTTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004175 GO:0004177 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005840 GO:0006464 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0008238 GO:0008240 GO:0009507 GO:0009536 GO:0009987 GO:0016020 GO:0016567 GO:0016604 GO:0016787 GO:0017171 GO:0019538 GO:0022626 GO:0031090 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0036211 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044445 GO:0044446 GO:0044451 GO:0044464 GO:0070011 GO:0070013 GO:0070647 GO:0071704 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_S8 TPPII

Protein Analysis

1375

Amino Acids

151.64

Weight (kDa)

6.07

Isoelectric Point (pI)

41.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_S8	PF00082	117 - 580	5.5e-45	Subtilase family
TPPII_Ig-like-1	PF21223	627 - 733	1.9e-22	Tripeptidyl-peptidase II, first Ig-like domain
TPPII_GBD	PF21316	753 - 837	7.1e-15	TPPII, galactose-binding domain-like
TPPII	PF12580	879 - 1063	1.2e-60	Tripeptidyl peptidase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20850
fragaria_vesca	FvH4_7g29630 FvH4_7g29630 FvH4_7g29630
malus_domestica	MD01G1203100.v1.1 MD07G1272600.v1.1
prunus_persica	Prupe.2G295900_v2.0.a1 Prupe.2G295900_v2.0.a1
pyrus_communis	pycom01g21240 pycom07g24900
rosa_chinensis	RchiOBHm_Chr1g0377641
rosa_laevigata	RLG00000026509
rosa_multiflora	Rmu_sc0002832.1_g000013
rosa_roxburghii	Rroxscaffold_4G00281150
rosa_rugosa	Rorug01G0403900
rosa_samantha	Rh1AG428700 Rh1BG380700 Rh1CG394700 Rh1DG411700
rosa_wichuraiana	Rw1G037000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	333
Acc36I	ACCTGC	1 cut(s)	777
Acc65I	GGTACC	1 cut(s)	1391
AccB1I	GGYRCC	3 cut(s)	356, 1391, 2044
AccB7I	CCANNNNNTGG	4 cut(s)	911, 1280, 2168, 2479
AccBSI	CCGCTC	1 cut(s)	3379
AccI	GTMKAC	2 cut(s)	1011, 2224
AccII	CGCG	4 cut(s)	354, 1165, 1885, 2844
AciI	CCGC	9 cut(s)	32, 210, 219, 225, 352, 354, 3272, 3379, 3957
AclWI	GGATC	5 cut(s)	396, 2093, 2106, 3211, 3653
AcoI	YGGCCR	3 cut(s)	349, 2470, 2696
AcsI	RAATTY	7 cut(s)	710, 1955, 2685, 2858, 3037, 3297, 3638
AcyI	GRCGYC	2 cut(s)	312, 357
AdeI	CACNNNGTG	1 cut(s)	1388
AflII	CTTAAG	1 cut(s)	3275
AgeI	ACCGGT	1 cut(s)	2659
AjnI	CCWGG	6 cut(s)	1126, 1384, 1471, 1549, 2263, 3133
AjuI	GAANNNNNNNTTGG	4 cut(s)	2283, 2315, 3998, 4030
Alw21I	GWGCWC	7 cut(s)	247, 1135, 1373, 1384, 1453, 1528, 1549
Alw26I	GTCTC	6 cut(s)	1152, 1175, 1432, 2642, 2910, 3695
AlwI	GGATC	5 cut(s)	396, 2093, 2106, 3211, 3653
AlwNI	CAGNNNCTG	1 cut(s)	3794
Ama87I	CYCGRG	1 cut(s)	1200
ApeKI	GCWGC	7 cut(s)	1214, 1270, 1442, 1556, 2438, 3013, 3593
ApoI	RAATTY	7 cut(s)	710, 1955, 2685, 2858, 3037, 3297, 3638
ArsI	GACNNNNNNTTYG	4 cut(s)	813, 845, 2843, 2875
AseI	ATTAAT	1 cut(s)	2574
AsiGI	ACCGGT	1 cut(s)	2659
Asp700I	GAANNNNTTC	2 cut(s)	3346, 4013
Asp718I	GGTACC	1 cut(s)	1391
AspLEI	GCGC	2 cut(s)	334, 359
AspS9I	GGNCC	8 cut(s)	766, 824, 1358, 1508, 2171, 2609, 3089, 3161
AsuC2I	CCSGG	2 cut(s)	460, 1820
AsuHPI	GGTGA	2 cut(s)	584, 3788
AsuII	TTCGAA	2 cut(s)	482, 2133
AsuNHI	GCTAGC	1 cut(s)	1970
AvaI	CYCGRG	1 cut(s)	1200
AvaII	GGWCC	2 cut(s)	766, 3161
BaeGI	GKGCMC	2 cut(s)	936, 2047
BalI	TGGCCA	1 cut(s)	2472
BamHI	GGATCC	1 cut(s)	2098
BanI	GGYRCC	3 cut(s)	356, 1391, 2044
BanII	GRGCYC	1 cut(s)	1528
BarI	GAAGNNNNNNTAC	4 cut(s)	3378, 3410, 4001, 4033
BbsI	GAAGAC	3 cut(s)	121, 2805, 3468
Bbv12I	GWGCWC	7 cut(s)	247, 1135, 1373, 1384, 1453, 1528, 1549
BbvCI	CCTCAGC	1 cut(s)	1674
BbvI	GCAGC	7 cut(s)	1201, 1257, 1429, 1568, 2425, 3025, 3605
BceAI	ACGGC	3 cut(s)	364, 824, 2981
BciT130I	CCWGG	6 cut(s)	1128, 1386, 1473, 1551, 2265, 3135
BciVI	GTATCC	1 cut(s)	610
BclI	TGATCA	2 cut(s)	715, 1239
BcnI	CCSGG	2 cut(s)	460, 1820
BcoDI	GTCTC	6 cut(s)	1152, 1175, 1432, 2642, 2910, 3695
BfaI	CTAG	7 cut(s)	440, 759, 1500, 1908, 1971, 2247, 3752
BfmI	CTRYAG	2 cut(s)	2208, 3786
BfoI	RGCGCY	1 cut(s)	360
BfrI	CTTAAG	1 cut(s)	3275
BfuAI	ACCTGC	1 cut(s)	777
BfuI	GTATCC	1 cut(s)	610
BglII	AGATCT	1 cut(s)	3826
BlpI	GCTNAGC	1 cut(s)	101
BmcAI	AGTACT	2 cut(s)	2592, 3924
Bme1390I	CCNGG	8 cut(s)	460, 1128, 1386, 1473, 1551, 1820, 2265, 3135
Bme18I	GGWCC	2 cut(s)	766, 3161
BmeT110I	CYCGRG	1 cut(s)	1200
BmgT120I	GGNCC	8 cut(s)	766, 824, 1358, 1508, 2171, 2609, 3089, 3161
BmiI	GGNNCC	8 cut(s)	358, 1104, 1393, 1992, 2046, 2100, 3220, 3955
BmrFI	CCNGG	8 cut(s)	460, 1128, 1386, 1473, 1551, 1820, 2265, 3135
BmrI	ACTGGG	2 cut(s)	5, 4101
BmsI	GCATC	9 cut(s)	319, 343, 542, 826, 976, 2878, 3391, 3580, 4077
BmtI	GCTAGC	1 cut(s)	1974
BmuI	ACTGGG	2 cut(s)	5, 4101
BpiI	GAAGAC	3 cut(s)	121, 2805, 3468
BpmI	CTGGAG	3 cut(s)	402, 1149, 1455
Bpu10I	CCTNAGC	2 cut(s)	1674, 2515
Bpu1102I	GCTNAGC	1 cut(s)	101
Bpu14I	TTCGAA	2 cut(s)	482, 2133
BpuEI	CTTGAG	4 cut(s)	914, 1505, 2805, 3467
BpuMI	CCSGG	2 cut(s)	460, 1820
Bsa29I	ATCGAT	4 cut(s)	319, 327, 391, 3715
BsaAI	YACGTR	1 cut(s)	3929
BsaBI	GATNNNNATC	1 cut(s)	333
BsaHI	GRCGYC	2 cut(s)	312, 357
BsaJI	CCNNGG	6 cut(s)	352, 687, 2162, 2946, 3120, 3134
BsaWI	WCCGGW	1 cut(s)	2659
BsaXI	ACNNNNNCTCC	8 cut(s)	127, 148, 157, 178, 2796, 2826, 3551, 3581
Bse118I	RCCGGY	3 cut(s)	2659, 2694, 2698
Bse1I	ACTGG	9 cut(s)	11, 1189, 1195, 1515, 1771, 2022, 2643, 3750, 4107
Bse3DI	GCAATG	5 cut(s)	195, 1443, 2916, 3509, 4088
Bse8I	GATNNNNATC	1 cut(s)	333
BseBI	CCWGG	6 cut(s)	1128, 1386, 1473, 1551, 2265, 3135
BseCI	ATCGAT	4 cut(s)	319, 327, 391, 3715
BseDI	CCNNGG	6 cut(s)	352, 687, 2162, 2946, 3120, 3134
BseGI	GGATG	9 cut(s)	334, 667, 742, 1828, 2256, 3058, 3167, 3406, 4092
BseJI	GATNNNNATC	1 cut(s)	333
BseMI	GCAATG	5 cut(s)	195, 1443, 2916, 3509, 4088
BseMII	CTCAG	6 cut(s)	1665, 2637, 2763, 3366, 3432, 3933
BseNI	ACTGG	9 cut(s)	11, 1189, 1195, 1515, 1771, 2022, 2643, 3750, 4107
BseSI	GKGCMC	2 cut(s)	936, 2047
BseX3I	CGGCCG	2 cut(s)	349, 2696
BseXI	GCAGC	7 cut(s)	1201, 1257, 1429, 1568, 2425, 3025, 3605
BsgI	GTGCAG	3 cut(s)	1575, 2108, 3822
Bsh1236I	CGCG	4 cut(s)	354, 1165, 1885, 2844
Bsh1285I	CGRYCG	3 cut(s)	319, 352, 2699
BshNI	GGYRCC	3 cut(s)	356, 1391, 2044
BshTI	ACCGGT	1 cut(s)	2659
BshVI	ATCGAT	4 cut(s)	319, 327, 391, 3715
BsiEI	CGRYCG	3 cut(s)	319, 352, 2699
BsiHKAI	GWGCWC	7 cut(s)	247, 1135, 1373, 1384, 1453, 1528, 1549
BsiHKCI	CYCGRG	1 cut(s)	1200
BsiSI	CCGG	5 cut(s)	459, 1819, 2660, 2695, 2699
BslFI	GGGAC	1 cut(s)	2370
BsmAI	GTCTC	6 cut(s)	1152, 1175, 1432, 2642, 2910, 3695
BsmFI	GGGAC	1 cut(s)	2370
BsmI	GAATGC	2 cut(s)	3149, 3455
BsoBI	CYCGRG	1 cut(s)	1200
Bsp119I	TTCGAA	2 cut(s)	482, 2133
Bsp1286I	GDGCHC	9 cut(s)	247, 936, 1135, 1373, 1384, 1453, 1528, 1549, 2047
Bsp1407I	TGTACA	1 cut(s)	992
Bsp1720I	GCTNAGC	1 cut(s)	101
Bsp19I	CCATGG	1 cut(s)	2162
Bsp68I	TCGCGA	1 cut(s)	1885
BspACI	CCGC	9 cut(s)	32, 210, 219, 225, 352, 354, 3272, 3379, 3957
BspCNI	CTCAG	6 cut(s)	1666, 2638, 2762, 3367, 3433, 3932
BspDI	ATCGAT	4 cut(s)	319, 327, 391, 3715
BspFNI	CGCG	4 cut(s)	354, 1165, 1885, 2844
BspHI	TCATGA	2 cut(s)	1602, 1960
BspLI	GGNNCC	8 cut(s)	358, 1104, 1393, 1992, 2046, 2100, 3220, 3955
BspMAI	CTGCAG	1 cut(s)	3790
BspMI	ACCTGC	1 cut(s)	777
BspOI	GCTAGC	1 cut(s)	1974
BspPI	GGATC	5 cut(s)	396, 2093, 2106, 3211, 3653
BspT104I	TTCGAA	2 cut(s)	482, 2133
BspT107I	GGYRCC	3 cut(s)	356, 1391, 2044
BspTI	CTTAAG	1 cut(s)	3275
BsrBI	CCGCTC	1 cut(s)	3379
BsrDI	GCAATG	5 cut(s)	195, 1443, 2916, 3509, 4088
BsrFI	RCCGGY	3 cut(s)	2659, 2694, 2698
BsrGI	TGTACA	1 cut(s)	992
BsrI	ACTGG	9 cut(s)	11, 1189, 1195, 1515, 1771, 2022, 2643, 3750, 4107
BssAI	RCCGGY	3 cut(s)	2659, 2694, 2698
BssECI	CCNNGG	6 cut(s)	352, 687, 2162, 2946, 3120, 3134
BssNAI	GTATAC	1 cut(s)	1012
BssNI	GRCGYC	2 cut(s)	312, 357
BssT1I	CCWWGG	3 cut(s)	687, 2162, 2946
Bst1107I	GTATAC	1 cut(s)	1012
Bst2UI	CCWGG	6 cut(s)	1128, 1386, 1473, 1551, 2265, 3135
Bst4CI	ACNGT	8 cut(s)	236, 728, 1375, 1700, 1939, 2235, 3107, 3614
Bst6I	CTCTTC	8 cut(s)	556, 1590, 1995, 2434, 3039, 3384, 3666, 3807
BstACI	GRCGYC	2 cut(s)	312, 357
BstAFI	CTTAAG	1 cut(s)	3275
BstAPI	GCANNNNNTGC	6 cut(s)	165, 1442, 1562, 1597, 2663, 4087
BstAUI	TGTACA	1 cut(s)	992
BstBAI	YACGTR	1 cut(s)	3929
BstBI	TTCGAA	2 cut(s)	482, 2133
BstC8I	GCNNGC	9 cut(s)	217, 1348, 1362, 1598, 1972, 3424, 3516, 3633, 3786
BstDSI	CCRYGG	2 cut(s)	352, 2162
BstF5I	GGATG	9 cut(s)	334, 667, 742, 1828, 2256, 3058, 3167, 3406, 4092
BstFNI	CGCG	4 cut(s)	354, 1165, 1885, 2844
BstH2I	RGCGCY	1 cut(s)	360
BstHHI	GCGC	2 cut(s)	334, 359
BstMAI	GTCTC	6 cut(s)	1152, 1175, 1432, 2642, 2910, 3695
BstMCI	CGRYCG	3 cut(s)	319, 352, 2699
BstNI	CCWGG	6 cut(s)	1128, 1386, 1473, 1551, 2265, 3135
BstNSI	RCATGY	2 cut(s)	584, 1600
BstSCI	CCNGG	8 cut(s)	458, 1126, 1384, 1471, 1549, 1818, 2263, 3133
BstSFI	CTRYAG	2 cut(s)	2208, 3786
BstSLI	GKGCMC	2 cut(s)	936, 2047
BstUI	CGCG	4 cut(s)	354, 1165, 1885, 2844
BstV1I	GCAGC	7 cut(s)	1201, 1257, 1429, 1568, 2425, 3025, 3605
BstV2I	GAAGAC	3 cut(s)	121, 2805, 3468
BstX2I	RGATCY	3 cut(s)	2098, 3203, 3826
BstXI	CCANNNNNNTGG	4 cut(s)	280, 1836, 3086, 3526
BstYI	RGATCY	3 cut(s)	2098, 3203, 3826
BstZ17I	GTATAC	1 cut(s)	1012
BstZI	CGGCCG	2 cut(s)	349, 2696
Bsu15I	ATCGAT	4 cut(s)	319, 327, 391, 3715
BsuI	GTATCC	1 cut(s)	610
BsuTUI	ATCGAT	4 cut(s)	319, 327, 391, 3715
BtgI	CCRYGG	2 cut(s)	352, 2162
BtgZI	GCGATG	3 cut(s)	349, 483, 1866
BtsCI	GGATG	9 cut(s)	334, 667, 742, 1828, 2256, 3058, 3167, 3406, 4092
BtsI	GCAGTG	5 cut(s)	148, 175, 1265, 1649, 1820
BtuMI	TCGCGA	1 cut(s)	1885
BveI	ACCTGC	1 cut(s)	777
Cac8I	GCNNGC	9 cut(s)	217, 1348, 1362, 1598, 1972, 3424, 3516, 3633, 3786
CaiI	CAGNNNCTG	1 cut(s)	3794
CciI	TCATGA	2 cut(s)	1602, 1960
CfoI	GCGC	2 cut(s)	334, 359
Cfr10I	RCCGGY	3 cut(s)	2659, 2694, 2698
Cfr13I	GGNCC	8 cut(s)	766, 824, 1358, 1508, 2171, 2609, 3089, 3161
Cfr42I	CCGCGG	1 cut(s)	355
ClaI	ATCGAT	4 cut(s)	319, 327, 391, 3715
CseI	GACGC	1 cut(s)	301
CsiI	ACCWGGT	1 cut(s)	1384
CspAI	ACCGGT	1 cut(s)	2659
CspCI	CAANNNNNGTGG	4 cut(s)	2520, 2555, 3585, 3620
DinI	GGCGCC	1 cut(s)	358
DraI	TTTAAA	1 cut(s)	3868
DraIII	CACNNNGTG	1 cut(s)	1388
EaeI	YGGCCR	3 cut(s)	349, 2470, 2696
EagI	CGGCCG	2 cut(s)	349, 2696
Eam1104I	CTCTTC	8 cut(s)	556, 1590, 1995, 2434, 3039, 3384, 3666, 3807
EarI	CTCTTC	8 cut(s)	556, 1590, 1995, 2434, 3039, 3384, 3666, 3807
EciI	GGCGGA	1 cut(s)	234
Ecl136II	GAGCTC	1 cut(s)	1526
EclXI	CGGCCG	2 cut(s)	349, 2696
Eco130I	CCWWGG	3 cut(s)	687, 2162, 2946
Eco147I	AGGCCT	1 cut(s)	2398
Eco24I	GRGCYC	1 cut(s)	1528
Eco47I	GGWCC	2 cut(s)	766, 3161
Eco52I	CGGCCG	2 cut(s)	349, 2696
Eco53kI	GAGCTC	1 cut(s)	1526
Eco88I	CYCGRG	1 cut(s)	1200
EcoICRI	GAGCTC	1 cut(s)	1526
EcoO109I	RGGNCCY	2 cut(s)	766, 824
EcoRI	GAATTC	1 cut(s)	2685
EcoRII	CCWGG	6 cut(s)	1126, 1384, 1471, 1549, 2263, 3133
EcoT14I	CCWWGG	3 cut(s)	687, 2162, 2946
EcoT38I	GRGCYC	1 cut(s)	1528
EgeI	GGCGCC	1 cut(s)	358
EheI	GGCGCC	1 cut(s)	358
ErhI	CCWWGG	3 cut(s)	687, 2162, 2946
FaqI	GGGAC	1 cut(s)	2370
FauNDI	CATATG	2 cut(s)	1423, 3244
FbaI	TGATCA	2 cut(s)	715, 1239
FblI	GTMKAC	2 cut(s)	1011, 2224
FokI	GGATG	9 cut(s)	321, 674, 749, 1835, 2263, 3065, 3154, 3413, 4099
FriOI	GRGCYC	1 cut(s)	1528
FspAI	RTGCGCAY	1 cut(s)	333
FspBI	CTAG	7 cut(s)	440, 759, 1500, 1908, 1971, 2247, 3752
FspI	TGCGCA	1 cut(s)	333
GlaI	GCGC	2 cut(s)	333, 358
GsuI	CTGGAG	3 cut(s)	402, 1149, 1455
HaeII	RGCGCY	1 cut(s)	360
HapII	CCGG	5 cut(s)	459, 1819, 2660, 2695, 2699
HgaI	GACGC	1 cut(s)	301
HhaI	GCGC	2 cut(s)	334, 359
Hin1I	GRCGYC	2 cut(s)	312, 357
Hin6I	GCGC	2 cut(s)	332, 357
HinP1I	GCGC	2 cut(s)	332, 357
HindIII	AAGCTT	3 cut(s)	2108, 2446, 3179
HpaI	GTTAAC	2 cut(s)	745, 2778
HpaII	CCGG	5 cut(s)	459, 1819, 2660, 2695, 2699
HphI	GGTGA	2 cut(s)	584, 3788
Hpy99I	CGWCG	3 cut(s)	236, 317, 350
HpyCH4III	ACNGT	8 cut(s)	236, 728, 1375, 1700, 1939, 2235, 3107, 3614
HpyCH4IV	ACGT	9 cut(s)	275, 620, 741, 2758, 2774, 3928, 3937, 3978, 4092
HpySE526I	ACGT	9 cut(s)	275, 620, 741, 2758, 2774, 3928, 3937, 3978, 4092
Hsp92I	GRCGYC	2 cut(s)	312, 357
HspAI	GCGC	2 cut(s)	332, 357
KasI	GGCGCC	1 cut(s)	356
KpnI	GGTACC	1 cut(s)	1395
Ksp22I	TGATCA	2 cut(s)	715, 1239
KspAI	GTTAAC	2 cut(s)	745, 2778
KspI	CCGCGG	1 cut(s)	355
Lsp1109I	GCAGC	7 cut(s)	1201, 1257, 1429, 1568, 2425, 3025, 3605
LweI	GCATC	9 cut(s)	319, 343, 542, 826, 976, 2878, 3391, 3580, 4077
MabI	ACCWGGT	1 cut(s)	1384
MaeI	CTAG	7 cut(s)	440, 759, 1500, 1908, 1971, 2247, 3752
MaeII	ACGT	9 cut(s)	275, 620, 741, 2758, 2774, 3928, 3937, 3978, 4092
MaeIII	GTNAC	9 cut(s)	45, 412, 1039, 2024, 2191, 2419, 2735, 2829, 2865
MbiI	CCGCTC	1 cut(s)	3379
MflI	RGATCY	3 cut(s)	2098, 3203, 3826
MhlI	GDGCHC	9 cut(s)	247, 936, 1135, 1373, 1384, 1453, 1528, 1549, 2047
MlsI	TGGCCA	1 cut(s)	2472
MluNI	TGGCCA	1 cut(s)	2472
Mly113I	GGCGCC	1 cut(s)	357
MmeI	TCCRAC	2 cut(s)	1357, 1942
Mox20I	TGGCCA	1 cut(s)	2472
MroXI	GAANNNNTTC	2 cut(s)	3346, 4013
MscI	TGGCCA	1 cut(s)	2472
MslI	CAYNNNNRTG	6 cut(s)	1230, 1601, 1965, 2022, 2093, 2985
Msp20I	TGGCCA	1 cut(s)	2472
MspA1I	CMGCKG	3 cut(s)	354, 1559, 3016
MspCI	CTTAAG	1 cut(s)	3275
MspI	CCGG	5 cut(s)	459, 1819, 2660, 2695, 2699
MspR9I	CCNGG	8 cut(s)	460, 1128, 1386, 1473, 1551, 1820, 2265, 3135
Mva1269I	GAATGC	2 cut(s)	3149, 3455
MvaI	CCWGG	6 cut(s)	1128, 1386, 1473, 1551, 2265, 3135
MvnI	CGCG	4 cut(s)	354, 1165, 1885, 2844
NarI	GGCGCC	1 cut(s)	357
NciI	CCSGG	2 cut(s)	460, 1820
NcoI	CCATGG	1 cut(s)	2162
NdeI	CATATG	2 cut(s)	1423, 3244
NheI	GCTAGC	1 cut(s)	1970
NlaIV	GGNNCC	8 cut(s)	358, 1104, 1393, 1992, 2046, 2100, 3220, 3955
NmuCI	GTSAC	4 cut(s)	45, 2024, 2735, 2865
NruI	TCGCGA	1 cut(s)	1885
NsbI	TGCGCA	1 cut(s)	333
NspI	RCATGY	2 cut(s)	584, 1600
NspV	TTCGAA	2 cut(s)	482, 2133
PaeI	GCATGC	1 cut(s)	1600
PaeR7I	CTCGAG	1 cut(s)	1200
PagI	TCATGA	2 cut(s)	1602, 1960
PceI	AGGCCT	1 cut(s)	2398
PcsI	WCGNNNNNNNCGW	2 cut(s)	372, 3934
PctI	GAATGC	2 cut(s)	3149, 3455
PdmI	GAANNNNTTC	2 cut(s)	3346, 4013
PfeI	GAWTC	5 cut(s)	321, 377, 1890, 2184, 3764
PflMI	CCANNNNNTGG	4 cut(s)	911, 1280, 2168, 2479
PinAI	ACCGGT	1 cut(s)	2659
Ple19I	CGATCG	1 cut(s)	319
PluTI	GGCGCC	1 cut(s)	360
Ppu21I	YACGTR	1 cut(s)	3929
PpuMI	RGGWCCY	1 cut(s)	766
PshBI	ATTAAT	1 cut(s)	2574
Psp124BI	GAGCTC	1 cut(s)	1528
Psp5II	RGGWCCY	1 cut(s)	766
Psp6I	CCWGG	6 cut(s)	1126, 1384, 1471, 1549, 2263, 3133
PspGI	CCWGG	6 cut(s)	1126, 1384, 1471, 1549, 2263, 3133
PspN4I	GGNNCC	8 cut(s)	358, 1104, 1393, 1992, 2046, 2100, 3220, 3955
PspPI	GGNCC	8 cut(s)	766, 824, 1358, 1508, 2171, 2609, 3089, 3161
PspPPI	RGGWCCY	1 cut(s)	766
PspXI	VCTCGAGB	1 cut(s)	1200
PstI	CTGCAG	1 cut(s)	3790
PstNI	CAGNNNCTG	1 cut(s)	3794
PsuI	RGATCY	3 cut(s)	2098, 3203, 3826
PvuI	CGATCG	1 cut(s)	319
PvuII	CAGCTG	2 cut(s)	1559, 3016
RruI	TCGCGA	1 cut(s)	1885
RseI	CAYNNNNRTG	6 cut(s)	1230, 1601, 1965, 2022, 2093, 2985
SacI	GAGCTC	1 cut(s)	1528
SacII	CCGCGG	1 cut(s)	355
SalI	GTCGAC	1 cut(s)	2223
Sau96I	GGNCC	8 cut(s)	766, 824, 1358, 1508, 2171, 2609, 3089, 3161
ScaI	AGTACT	2 cut(s)	2592, 3924
ScrFI	CCNGG	8 cut(s)	460, 1128, 1386, 1473, 1551, 1820, 2265, 3135
SduI	GDGCHC	9 cut(s)	247, 936, 1135, 1373, 1384, 1453, 1528, 1549, 2047
SexAI	ACCWGGT	1 cut(s)	1384
SfaNI	GCATC	9 cut(s)	319, 343, 542, 826, 976, 2878, 3391, 3580, 4077
SfcI	CTRYAG	2 cut(s)	2208, 3786
SfoI	GGCGCC	1 cut(s)	358
Sfr274I	CTCGAG	1 cut(s)	1200
Sfr303I	CCGCGG	1 cut(s)	355
SfuI	TTCGAA	2 cut(s)	482, 2133
SgrBI	CCGCGG	1 cut(s)	355
SinI	GGWCC	2 cut(s)	766, 3161
SlaI	CTCGAG	1 cut(s)	1200
SmiMI	CAYNNNNRTG	6 cut(s)	1230, 1601, 1965, 2022, 2093, 2985
SmlI	CTYRAG	6 cut(s)	893, 1200, 1484, 2820, 3275, 3482
SmoI	CTYRAG	6 cut(s)	893, 1200, 1484, 2820, 3275, 3482
SphI	GCATGC	1 cut(s)	1600
SseBI	AGGCCT	1 cut(s)	2398
SsiI	CCGC	9 cut(s)	32, 210, 219, 225, 352, 354, 3272, 3379, 3957
SspDI	GGCGCC	1 cut(s)	356
SspI	AATATT	2 cut(s)	1332, 3525
SspMI	CTAG	7 cut(s)	440, 759, 1500, 1908, 1971, 2247, 3752
SstI	GAGCTC	1 cut(s)	1528
StuI	AGGCCT	1 cut(s)	2398
StyD4I	CCNGG	8 cut(s)	458, 1126, 1384, 1471, 1549, 1818, 2263, 3133
StyI	CCWWGG	3 cut(s)	687, 2162, 2946
TaaI	ACNGT	8 cut(s)	236, 728, 1375, 1700, 1939, 2235, 3107, 3614
TaiI	ACGT	9 cut(s)	278, 623, 744, 2761, 2777, 3931, 3940, 3981, 4095
TaqII	GACCGA	1 cut(s)	133
TatI	WGTACW	6 cut(s)	992, 1488, 2590, 2955, 3535, 3922
TauI	GCSGC	3 cut(s)	34, 354, 357
TfiI	GAWTC	5 cut(s)	321, 377, 1890, 2184, 3764
TseFI	GTSAC	4 cut(s)	45, 2024, 2735, 2865
TseI	GCWGC	7 cut(s)	1214, 1270, 1442, 1556, 2438, 3013, 3593
Tsp45I	GTSAC	4 cut(s)	45, 2024, 2735, 2865
TspGWI	ACGGA	4 cut(s)	629, 2817, 2817, 3924
Van91I	CCANNNNNTGG	4 cut(s)	911, 1280, 2168, 2479
Vha464I	CTTAAG	1 cut(s)	3275
VpaK11BI	GGWCC	2 cut(s)	766, 3161
VspI	ATTAAT	1 cut(s)	2574
XapI	RAATTY	7 cut(s)	710, 1955, 2685, 2858, 3037, 3297, 3638
XceI	RCATGY	2 cut(s)	584, 1600
XhoI	CTCGAG	1 cut(s)	1200
XmiI	GTMKAC	2 cut(s)	1011, 2224
XmnI	GAANNNNTTC	2 cut(s)	3346, 4013
XspI	CTAG	7 cut(s)	440, 759, 1500, 1908, 1971, 2247, 3752
ZrmI	AGTACT	2 cut(s)	2592, 3924

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.