pycom07g27650

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008723
Physical Location & Seq
Forward (+)
27293511 .. 27294071
561 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g27650.1

Sequence Viewer

Length: 561 bp
ATGGACTTTCAGAATAATTCTCAGCTCGAATCGGGGAAAATGGAACAGATTGTTTCCCAATTTCTATTGAAAAGCTTGCATATTGTTTTGGACTCTAGGATTCCTTCTCTTCACCCACATGATCGCAGTGGTGACCTGTCATCTGCAACTCAAGTGAGAAAGAGTGACAAGTGGTTTAACTTAATACTAGGAGACCGCCCTGCAGCTCTTGAGAATTTAAATTTTTGGCACAGAAGTGTAATGGATCCTATGATAATTGACATTATACTTGTTCGCGGAGGACCTAGTTCTTCTGTTGACAATCTGTATGCGAATACAGTGGAGGGGACGTCTGTTGAGACAATCATAGAGAGGTGGGTTGTCCAGTACGAAACTCCAAGGGCTGTGGCTCCCCAAACTGGTGACACTTCAGCCTCTTACAAAAAGACATACAAGAAGTCAATCATTCTTTTACGTACTCTTTATTCGTATATGAGACTTCTTCCAGCTTATAGGATCTCCAGACAGCTAAGTACATCTAGTCAGACTTACAATTTTGATATTATCTACAAGGTGTCTTAA

Protein Analysis

187

Amino Acids

21.23

Weight (kDa)

9.1

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG13 PF10033 18 - 185 1.3e-30 Autophagy-related protein 13
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 332
AccII CGCG 1 cut(s) 276
AciI CCGC 2 cut(s) 196, 276
AclWI GGATC 3 cut(s) 239, 252, 503
AcsI RAATTY 2 cut(s) 214, 220
AcuI CTGAAG 1 cut(s) 393
AcyI GRCGYC 1 cut(s) 329
AfaI GTAC 3 cut(s) 368, 457, 514
AfiI CCNNNNNNNGG 1 cut(s) 398
AgsI TTSAA 1 cut(s) 70
AleI CACNNNNGTG 1 cut(s) 234
AluBI AGCT 5 cut(s) 25, 75, 206, 488, 508
AluI AGCT 5 cut(s) 25, 75, 206, 488, 508
Alw26I GTCTC 3 cut(s) 186, 332, 469
AlwI GGATC 3 cut(s) 239, 252, 503
ApeKI GCWGC 1 cut(s) 203
ApoI RAATTY 2 cut(s) 214, 220
AspS9I GGNCC 1 cut(s) 281
AsuHPI GGTGA 3 cut(s) 104, 143, 413
AvaII GGWCC 1 cut(s) 281
BamHI GGATCC 1 cut(s) 244
BbvI GCAGC 1 cut(s) 215
BcoDI GTCTC 3 cut(s) 186, 332, 469
BfaI CTAG 4 cut(s) 96, 188, 285, 519
BfmI CTRYAG 1 cut(s) 201
BisI GCNGC 1 cut(s) 204
BlsI GCNGC 1 cut(s) 205
Bme18I GGWCC 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 281
BmiI GGNNCC 2 cut(s) 246, 390
BpmI CTGGAG 1 cut(s) 484
BpuEI CTTGAG 2 cut(s) 135, 230
BsaAI YACGTR 1 cut(s) 455
BsaHI GRCGYC 1 cut(s) 329
BsaI GGTCTC 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 377
BsaXI ACNNNNNCTCC 2 cut(s) 314, 344
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse1I ACTGG 2 cut(s) 364, 403
BseDI CCNNGG 1 cut(s) 377
BseLI CCNNNNNNNGG 1 cut(s) 398
BseMII CTCAG 1 cut(s) 35
BseNI ACTGG 2 cut(s) 364, 403
BseXI GCAGC 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 276
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 1 cut(s) 398
BsmAI GTCTC 3 cut(s) 186, 332, 469
BsmFI GGGAC 1 cut(s) 340
Bso31I GGTCTC 1 cut(s) 186
Bsp143I GATC 3 cut(s) 121, 244, 495
BspACI CCGC 2 cut(s) 196, 276
BspCNI CTCAG 1 cut(s) 34
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 2 cut(s) 246, 390
BspMAI CTGCAG 1 cut(s) 205
BspPI GGATC 3 cut(s) 239, 252, 503
BspTNI GGTCTC 1 cut(s) 186
BsrI ACTGG 2 cut(s) 364, 403
BssECI CCNNGG 1 cut(s) 377
BssMI GATC 3 cut(s) 121, 244, 495
BssNI GRCGYC 1 cut(s) 329
BssT1I CCWWGG 1 cut(s) 377
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 1 cut(s) 114
BstACI GRCGYC 1 cut(s) 329
BstBAI YACGTR 1 cut(s) 455
BstC8I GCNNGC 1 cut(s) 77
BstDEI CTNAG 2 cut(s) 21, 509
BstEII GGTNACC 1 cut(s) 131
BstFNI CGCG 1 cut(s) 276
BstKTI GATC 3 cut(s) 124, 247, 498
BstMAI GTCTC 3 cut(s) 186, 332, 469
BstMBI GATC 3 cut(s) 121, 244, 495
BstPI GGTNACC 1 cut(s) 131
BstSFI CTRYAG 1 cut(s) 201
BstSNI TACGTA 1 cut(s) 455
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 215
BstX2I RGATCY 2 cut(s) 244, 495
BstYI RGATCY 2 cut(s) 244, 495
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 2 cut(s) 133, 324
Cac8I GCNNGC 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 281
Csp6I GTAC 3 cut(s) 367, 456, 513
CspCI CAANNNNNGTGG 2 cut(s) 366, 401
CviAII CATG 1 cut(s) 119
CviJI RGCY 8 cut(s) 25, 75, 206, 383, 389, 413, 488, 508
CviKI_1 RGCY 8 cut(s) 25, 75, 206, 383, 389, 413, 488, 508
CviQI GTAC 3 cut(s) 367, 456, 513
DdeI CTNAG 2 cut(s) 21, 509
DpnI GATC 3 cut(s) 123, 246, 497
DpnII GATC 3 cut(s) 121, 244, 495
DraI TTTAAA 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Eco105I TACGTA 1 cut(s) 455
Eco130I CCWWGG 1 cut(s) 377
Eco31I GGTCTC 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 281
Eco57I CTGAAG 1 cut(s) 393
Eco91I GGTNACC 1 cut(s) 131
EcoO109I RGGNCCY 1 cut(s) 281
EcoO65I GGTNACC 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 377
ErhI CCWWGG 1 cut(s) 377
FaeI CATG 1 cut(s) 122
FaqI GGGAC 1 cut(s) 340
FatI CATG 1 cut(s) 118
Fnu4HI GCNGC 1 cut(s) 204
Fsp4HI GCNGC 1 cut(s) 204
FspBI CTAG 4 cut(s) 96, 188, 285, 519
GluI GCNGC 1 cut(s) 204
GsuI CTGGAG 1 cut(s) 484
Hin1I GRCGYC 1 cut(s) 329
Hin1II CATG 1 cut(s) 122
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HindIII AAGCTT 1 cut(s) 73
HinfI GANTC 3 cut(s) 29, 92, 100
HphI GGTGA 3 cut(s) 104, 143, 413
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 2 cut(s) 12, 525
Hpy188III TCNNGA 2 cut(s) 209, 501
Hpy8I GTNNAC 1 cut(s) 298
HpyAV CCTTC 1 cut(s) 114
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 2 cut(s) 329, 454
HpyCH4V TGCA 3 cut(s) 79, 146, 203
HpyF3I CTNAG 2 cut(s) 21, 509
HpySE526I ACGT 2 cut(s) 329, 454
Hsp92I GRCGYC 1 cut(s) 329
Hsp92II CATG 1 cut(s) 122
Kzo9I GATC 3 cut(s) 121, 244, 495
LmnI GCTCC 1 cut(s) 394
LpnPI CCDG 6 cut(s) 149, 213, 377, 384, 498, 514
Lsp1109I GCAGC 1 cut(s) 215
MaeI CTAG 4 cut(s) 96, 188, 285, 519
MaeII ACGT 2 cut(s) 329, 454
MaeIII GTNAC 3 cut(s) 131, 164, 401
MalI GATC 3 cut(s) 123, 246, 497
MboI GATC 3 cut(s) 121, 244, 495
MboII GAAGA 3 cut(s) 101, 282, 473
MflI RGATCY 2 cut(s) 244, 495
MluCI AATT 6 cut(s) 16, 59, 214, 220, 255, 532
MlyI GAGTC 1 cut(s) 86
MnlI CCTC 4 cut(s) 272, 316, 345, 424
MseI TTAA 4 cut(s) 177, 182, 218, 559
MslI CAYNNNNRTG 2 cut(s) 117, 234
MvnI CGCG 1 cut(s) 276
NdeII GATC 3 cut(s) 121, 244, 495
NlaIII CATG 1 cut(s) 122
NlaIV GGNNCC 2 cut(s) 246, 390
NmuCI GTSAC 3 cut(s) 131, 164, 401
OliI CACNNNNGTG 1 cut(s) 234
PfeI GAWTC 2 cut(s) 29, 100
PkrI GCNGC 1 cut(s) 205
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
Ppu21I YACGTR 1 cut(s) 455
PpuMI RGGWCCY 1 cut(s) 281
Psp5II RGGWCCY 1 cut(s) 281
PspEI GGTNACC 1 cut(s) 131
PspN4I GGNNCC 2 cut(s) 246, 390
PspPI GGNCC 1 cut(s) 281
PspPPI RGGWCCY 1 cut(s) 281
PstI CTGCAG 1 cut(s) 205
PsuI RGATCY 2 cut(s) 244, 495
RsaI GTAC 3 cut(s) 368, 457, 514
RsaNI GTAC 3 cut(s) 367, 456, 513
RseI CAYNNNNRTG 2 cut(s) 117, 234
SaqAI TTAA 4 cut(s) 177, 182, 218, 559
SatI GCNGC 1 cut(s) 204
Sau3AI GATC 3 cut(s) 121, 244, 495
Sau96I GGNCC 1 cut(s) 281
SchI GAGTC 1 cut(s) 86
SfcI CTRYAG 1 cut(s) 201
SinI GGWCC 1 cut(s) 281
SmiI ATTTAAAT 1 cut(s) 219
SmiMI CAYNNNNRTG 2 cut(s) 117, 234
SmlI CTYRAG 2 cut(s) 150, 209
SmoI CTYRAG 2 cut(s) 150, 209
SnaBI TACGTA 1 cut(s) 455
Sse9I AATT 6 cut(s) 16, 59, 214, 220, 255, 532
SsiI CCGC 2 cut(s) 196, 276
SspMI CTAG 4 cut(s) 96, 188, 285, 519
StyI CCWWGG 1 cut(s) 377
SwaI ATTTAAAT 1 cut(s) 219
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 2 cut(s) 332, 457
TaqI TCGA 1 cut(s) 27
TasI AATT 6 cut(s) 16, 59, 214, 220, 255, 532
TatI WGTACW 1 cut(s) 512
TfiI GAWTC 2 cut(s) 29, 100
Tru1I TTAA 4 cut(s) 177, 182, 218, 559
Tru9I TTAA 4 cut(s) 177, 182, 218, 559
TscAI CASTG 2 cut(s) 133, 324
TseFI GTSAC 3 cut(s) 131, 164, 401
TseI GCWGC 1 cut(s) 203
Tsp45I GTSAC 3 cut(s) 131, 164, 401
TspRI CASTG 2 cut(s) 133, 324
VpaK11BI GGWCC 1 cut(s) 281
XapI RAATTY 2 cut(s) 214, 220
XspI CTAG 4 cut(s) 96, 188, 285, 519
ZraI GACGTC 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.