Rorug01G0440500

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008723
Physical Location & Seq
Reverse (-)
53865269 .. 53865832
564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0440500.1

Sequence Viewer

Length: 564 bp
ATGCAGATTCGGGTGAAGTGCAGTTGTGGAGAGAATTGTCCAGAATGGGCCATGCTTGAGTTACAAGGAATGGTTGAAGTTCAGCCCCAGTTCAAAGATCGTCTCCAGAATCTGAAGATTGGCCTCCTCTGCCGCCCTTCCTCGCCGGAAACCTACACATTCACCGTAGGTTATCATGAACTGACCGGGACGAAGCTGCCGCTGAAGAAGCCGCTCTTGGTGTTGAACAAAGTCAAGGATGCCGGTGAGGAGGGTAGTTCGGGGAGGGTGGAATTGGAAGTCATTGGAATCATCCGCCACCGGATTCTGTTCAAGACCAGACCTAAGGCCCTTATTTCCAGTAAGCTTTCGATTCCATTCTGTTTGTTTTTGCAGTTGACTTGTGGTGACATTGGTTTTCTTTGTTTTGCAAAGAAGCACAGCCTACAGCGAAGGAAAGAGCTACTCTTGCATCAAGCAAATAGCAGGTTTACAGGCAGAGCGCCATGGACAACTGGAAAAGTTTCCGTATCGAATATTTATATGTATTTTGTATGCTTCTCGTTGAAAAGAAGCAAGCTTTGA

Protein Analysis

187

Amino Acids

21.26

Weight (kDa)

9.72

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ctf8 PF09696 40 - 109 4.1e-07 Ctf8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 456
AccBSI CCGCTC 1 cut(s) 214
AciI CCGC 4 cut(s) 133, 200, 212, 295
AcuI CTGAAG 2 cut(s) 134, 224
AgsI TTSAA 5 cut(s) 77, 94, 226, 313, 547
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 4 cut(s) 196, 346, 442, 559
AluI AGCT 4 cut(s) 196, 346, 442, 559
Alw26I GTCTC 1 cut(s) 107
AlwNI CAGNNNCTG 1 cut(s) 112
AoxI GGCC 3 cut(s) 48, 121, 327
ApeKI GCWGC 1 cut(s) 196
Asp700I GAANNNNTTC 1 cut(s) 502
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 2 cut(s) 48, 328
AsuC2I CCSGG 1 cut(s) 187
AsuHPI GGTGA 4 cut(s) 25, 154, 257, 398
AxyI CCTNAGG 1 cut(s) 324
BbvI GCAGC 1 cut(s) 183
BcnI CCSGG 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 425
BfoI RGCGCY 1 cut(s) 485
BfuAI ACCTGC 1 cut(s) 456
BisI GCNGC 4 cut(s) 133, 197, 200, 212
BlsI GCNGC 4 cut(s) 134, 198, 201, 213
Bme1390I CCNGG 1 cut(s) 187
BmgT120I GGNCC 2 cut(s) 48, 328
BmrFI CCNGG 1 cut(s) 187
BmrI ACTGGG 1 cut(s) 82
BmsI GCATC 2 cut(s) 229, 460
BmuI ACTGGG 1 cut(s) 82
BpmI CTGGAG 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 187
BsaJI CCNNGG 1 cut(s) 485
BsaWI WCCGGW 1 cut(s) 300
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 3 cut(s) 88, 339, 499
Bse21I CCTNAGG 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 485
BseGI GGATG 2 cut(s) 244, 291
BseNI ACTGG 3 cut(s) 88, 339, 499
BseRI GAGGAG 2 cut(s) 116, 263
BseXI GCAGC 1 cut(s) 183
BsgI GTGCAG 1 cut(s) 40
BshFI GGCC 3 cut(s) 50, 123, 329
BsiSI CCGG 4 cut(s) 146, 186, 243, 301
BslFI GGGAC 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 107
BsmBI CGTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 202
BsnI GGCC 3 cut(s) 50, 123, 329
Bsp143I GATC 1 cut(s) 97
Bsp19I CCATGG 1 cut(s) 485
BspACI CCGC 4 cut(s) 133, 200, 212, 295
BspANI GGCC 3 cut(s) 50, 123, 329
BspHI TCATGA 1 cut(s) 175
BspMI ACCTGC 1 cut(s) 456
BsrBI CCGCTC 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 3 cut(s) 88, 339, 499
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 1 cut(s) 485
BssMI GATC 1 cut(s) 97
BssT1I CCWWGG 1 cut(s) 485
Bst4CI ACNGT 1 cut(s) 166
BstC8I GCNNGC 1 cut(s) 557
BstDEI CTNAG 1 cut(s) 324
BstDSI CCRYGG 1 cut(s) 485
BstF5I GGATG 2 cut(s) 244, 291
BstH2I RGCGCY 1 cut(s) 485
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 1 cut(s) 100
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 1 cut(s) 97
BstMWI GCNNNNNNNGC 3 cut(s) 129, 208, 448
BstSCI CCNGG 1 cut(s) 185
BstSFI CTRYAG 1 cut(s) 425
BstV1I GCAGC 1 cut(s) 183
Bsu36I CCTNAGG 1 cut(s) 324
BsuRI GGCC 3 cut(s) 50, 123, 329
BtgI CCRYGG 1 cut(s) 485
BtsCI GGATG 2 cut(s) 244, 291
BveI ACCTGC 1 cut(s) 456
Cac8I GCNNGC 1 cut(s) 557
CaiI CAGNNNCTG 1 cut(s) 112
CciI TCATGA 1 cut(s) 175
CfoI GCGC 1 cut(s) 484
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 48, 328
CviAII CATG 3 cut(s) 52, 176, 486
DdeI CTNAG 1 cut(s) 324
DpnI GATC 1 cut(s) 99
DpnII GATC 1 cut(s) 97
EciI GGCGGA 1 cut(s) 284
Eco130I CCWWGG 1 cut(s) 485
Eco57I CTGAAG 2 cut(s) 134, 224
Eco81I CCTNAGG 1 cut(s) 324
EcoO109I RGGNCCY 1 cut(s) 328
EcoT14I CCWWGG 1 cut(s) 485
ErhI CCWWGG 1 cut(s) 485
Esp3I CGTCTC 1 cut(s) 107
FaeI CATG 3 cut(s) 55, 179, 489
FaiI YATR 6 cut(s) 53, 177, 487, 522, 524, 535
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 1 cut(s) 202
FatI CATG 3 cut(s) 51, 175, 485
Fnu4HI GCNGC 4 cut(s) 133, 197, 200, 212
FokI GGATG 2 cut(s) 251, 278
Fsp4HI GCNGC 4 cut(s) 133, 197, 200, 212
GlaI GCGC 1 cut(s) 483
GluI GCNGC 4 cut(s) 133, 197, 200, 212
GsuI CTGGAG 1 cut(s) 89
HaeII RGCGCY 1 cut(s) 485
HaeIII GGCC 3 cut(s) 50, 123, 329
HapII CCGG 4 cut(s) 146, 186, 243, 301
HhaI GCGC 1 cut(s) 484
Hin1II CATG 3 cut(s) 55, 179, 489
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HindIII AAGCTT 2 cut(s) 344, 557
HinfI GANTC 5 cut(s) 7, 109, 288, 304, 352
HpaII CCGG 4 cut(s) 146, 186, 243, 301
HphI GGTGA 4 cut(s) 25, 154, 257, 398
Hpy166II GTNNAC 2 cut(s) 378, 471
Hpy188I TCNGA 1 cut(s) 114
Hpy188III TCNNGA 4 cut(s) 41, 106, 176, 313
Hpy8I GTNNAC 2 cut(s) 378, 471
HpyAV CCTTC 2 cut(s) 147, 426
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4V TGCA 5 cut(s) 4, 21, 373, 410, 451
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 208, 448
HpyF3I CTNAG 1 cut(s) 324
Hsp92II CATG 3 cut(s) 55, 179, 489
HspAI GCGC 1 cut(s) 482
Kzo9I GATC 1 cut(s) 97
Lsp1109I GCAGC 1 cut(s) 183
LweI GCATC 2 cut(s) 229, 460
MaeIII GTNAC 2 cut(s) 60, 386
MalI GATC 1 cut(s) 99
MbiI CCGCTC 1 cut(s) 214
MboI GATC 1 cut(s) 97
MboII GAAGA 2 cut(s) 127, 217
MluCI AATT 2 cut(s) 34, 272
MnlI CCTC 6 cut(s) 134, 137, 151, 241, 244, 258
MroXI GAANNNNTTC 1 cut(s) 502
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 4 cut(s) 146, 186, 243, 301
MspR9I CCNGG 1 cut(s) 187
MwoI GCNNNNNNNGC 3 cut(s) 129, 208, 448
NciI CCSGG 1 cut(s) 187
NcoI CCATGG 1 cut(s) 485
NdeII GATC 1 cut(s) 97
NlaIII CATG 3 cut(s) 55, 179, 489
NmuCI GTSAC 1 cut(s) 386
PagI TCATGA 1 cut(s) 175
PdmI GAANNNNTTC 1 cut(s) 502
PfeI GAWTC 5 cut(s) 7, 109, 288, 304, 352
PkrI GCNGC 4 cut(s) 134, 198, 201, 213
PspPI GGNCC 2 cut(s) 48, 328
PstNI CAGNNNCTG 1 cut(s) 112
SatI GCNGC 4 cut(s) 133, 197, 200, 212
Sau3AI GATC 1 cut(s) 97
Sau96I GGNCC 2 cut(s) 48, 328
ScrFI CCNGG 1 cut(s) 187
SetI ASST 8 cut(s) 155, 172, 198, 325, 348, 444, 470, 561
SfaNI GCATC 2 cut(s) 229, 460
SfcI CTRYAG 1 cut(s) 425
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
Sse9I AATT 2 cut(s) 34, 272
SsiI CCGC 4 cut(s) 133, 200, 212, 295
SspI AATATT 1 cut(s) 517
StyD4I CCNGG 1 cut(s) 185
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 1 cut(s) 166
TaqI TCGA 2 cut(s) 350, 512
TasI AATT 2 cut(s) 34, 272
TauI GCSGC 3 cut(s) 135, 202, 214
TfiI GAWTC 5 cut(s) 7, 109, 288, 304, 352
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 1 cut(s) 196
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 1 cut(s) 192
TspGWI ACGGA 1 cut(s) 496
XmnI GAANNNNTTC 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.