Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0008723

Physical Location & Seq

Genomic Coordinates Forward (+)

27294180 .. 27296633

Gene Structure 2454 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g27660.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1185 bp

ATGCTAGCTGATTTCAATCTTGAGCCTTCAGCACCAATGCCACCGAGGATAATTACTGATTATGTTGGTAGTCCTGCCGCTGACCCCCTGAGGTCTTTCCCCTCATCAGGGAAGGGTTTTAGTGCAACTTCCTTTCTGTCGAGAGGAGCACGACCGCCATCCAGTGTGCCACACCAACGCCCACACAGCTGGACAAGTGGCTTCCACAGGCCACCTCCTTTTATTCACAATAGACCCTTAGTTGGATCTCCACCTGCATATCGTGCATCTCCTATGTCACATGATGTTGGATCTCCTCCTATTGATACATTTGCCAACAGAGGTCAAAACTATAGACCTCTAAGTCATAAAAGAAATATGAGTTCAGATGAGTATCAGCTTTCACCTCCATTTTCGCCATCCCCGTCGCCATCACCCCCAGCATACTTGTCCGGTGGTAATTTCAATCCTATGCAAACTCGAATTCGTTCAGGAACTGCCCCTGTAAGCATCCCCCTTCCAATGGGGGGCCCTAGATACTTATCCCCCAATTTTTCTGATCCAAGTAGAAATCCTCTTCCTCCTTTATCACCCAGAAGCACAAGAAATGATCATTCCTCTCAAGAGTCTCCATCTGGAATCAGGGCAATCAGAAAGTTGGATGTTTCAAGGGCCGGAGAGACTCACCCTGGAAGTTCAAATCATTACATTGGTCAGAAGGTGATGAAAGACAGCAAAGATGATTCAGGGCGTTTCTCAGGATTGTTGTCATCTAGTGGCTCACCACGTGTTGGATTTTCTAGAAGCTCGAGTAGATTGTCTTTCCAGGATGATTTGGATGATTTTGAGTTCTCATGTCCGTTTGACGTGGATGATGTTGATACACCAGATTCTCAAGCCAGTGATGGCAAAAGGCCTTCAGAGTTCATATCTCAGTCACTGCCAATGGGTAGAAAATCACAAGATGCTGCTGTCGGCGTTCTGGTTCAGATGCTGAGGACAGCTCCGCCTCTGCGCCAAGATTCAAGTTGTTACTCATCCCAGTCTGGGAAGAATGAGCATGAGGGAGGAGTTGCTACGGCTTCTGGTTTCTTTATGCCGCGGAAGACATCCGATGCACTGGAGGAGCTCAGAAGCTACCGAGATATGAAAGACCTTCTACTCTCCAAGAGCGGAACGCGGGTGCTTGCCAAAGACGAATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005776 GO:0006914 GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044248 GO:0044424 GO:0044444 GO:0044464 GO:0061919

KEGG Pathways

Metabolic & Signaling

ko04136 ko04138 ko04140 ko04211 map04136 map04138 map04140 map04211

Pfam Domains

Protein Families

ATG13

Protein Analysis

395

Amino Acids

42.73

Weight (kDa)

9.29

Isoelectric Point (pI)

72.82

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008723)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G49590 AT3G49590 AT3G49590
fragaria_vesca	FvH4_7g33350 FvH4_7g33350 FvH4_7g33680
malus_domestica	MD01G1231700.v1.1 MD07G1303800.v1.1
prunus_persica	Prupe.2G322400_v2.0.a1 Prupe.2G322400_v2.0.a1
pyrus_communis	pycom01g23960 pycom07g27650 pycom07g27660
rosa_chinensis	RchiOBHm_Chr1g0383331
rosa_laevigata	RLG00000026112
rosa_multiflora	Rmu_sc0007173.1_g000015
rosa_roxburghii	Rroxscaffold_4G00277390
rosa_rugosa	Rorug01G0440500
rosa_samantha	Rh1AG466400 Rh1BG423700 Rh1CG436900 Rh1DG456400
rosa_wichuraiana	Rw1G040610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	262
AasI	GACNNNNNNGTC	1 cut(s)	342
Acc36I	ACCTGC	1 cut(s)	262
AccB7I	CCANNNNNTGG	1 cut(s)	770
AccBSI	CCGCTC	1 cut(s)	1152
AccII	CGCG	2 cut(s)	1081, 1159
AciI	CCGC	7 cut(s)	78, 155, 986, 1079, 1081, 1152, 1159
AclWI	GGATC	3 cut(s)	253, 298, 533
AcsI	RAATTY	1 cut(s)	462
AcuI	CTGAAG	2 cut(s)	12, 882
AcvI	CACGTG	1 cut(s)	767
AdeI	CACNNNGTG	1 cut(s)	767
AfiI	CCNNNNNNNGG	7 cut(s)	107, 108, 242, 502, 506, 770, 1026
AflIII	ACRYGT	1 cut(s)	766
AgsI	TTSAA	5 cut(s)	16, 445, 648, 678, 1005
AjiI	CACGTC	1 cut(s)	847
AjnI	CCWGG	2 cut(s)	667, 804
AluBI	AGCT	7 cut(s)	8, 189, 379, 786, 983, 1108, 1116
AluI	AGCT	7 cut(s)	8, 189, 379, 786, 983, 1108, 1116
Alw21I	GWGCWC	2 cut(s)	151, 1110
Alw26I	GTCTC	2 cut(s)	612, 653
AlwI	GGATC	3 cut(s)	253, 298, 533
AlwNI	CAGNNNCTG	3 cut(s)	476, 919, 973
Ama87I	CYCGRG	1 cut(s)	787
AoxI	GGCC	4 cut(s)	209, 508, 651, 893
ApaI	GGGCCC	1 cut(s)	512
ApeKI	GCWGC	1 cut(s)	947
ApoI	RAATTY	1 cut(s)	462
Asp700I	GAANNNNTTC	1 cut(s)	466
AspLEI	GCGC	1 cut(s)	996
AspS9I	GGNCC	3 cut(s)	508, 509, 651
AsuHPI	GGTGA	6 cut(s)	375, 405, 561, 656, 712, 753
AsuNHI	GCTAGC	1 cut(s)	4
AvaI	CYCGRG	1 cut(s)	787
AxyI	CCTNAGG	1 cut(s)	89
BaeGI	GKGCMC	1 cut(s)	512
BanII	GRGCYC	2 cut(s)	512, 1110
BbrPI	CACGTG	1 cut(s)	767
BbsI	GAAGAC	1 cut(s)	1091
Bbv12I	GWGCWC	2 cut(s)	151, 1110
BbvCI	CCTCAGC	1 cut(s)	974
BbvI	GCAGC	1 cut(s)	934
BccI	CCATC	5 cut(s)	166, 406, 418, 619, 878
BceAI	ACGGC	1 cut(s)	1074
BciT130I	CCWGG	2 cut(s)	669, 806
BclI	TGATCA	1 cut(s)	589
BcoDI	GTCTC	2 cut(s)	612, 653
BfaI	CTAG	4 cut(s)	5, 513, 753, 780
BfmI	CTRYAG	1 cut(s)	331
BfuAI	ACCTGC	1 cut(s)	262
BisI	GCNGC	3 cut(s)	78, 948, 1079
BlsI	GCNGC	3 cut(s)	79, 949, 1080
Bme1390I	CCNGG	2 cut(s)	669, 806
BmeT110I	CYCGRG	1 cut(s)	787
BmgBI	CACGTC	1 cut(s)	847
BmgT120I	GGNCC	3 cut(s)	508, 509, 651
BmiI	GGNNCC	2 cut(s)	509, 510
BmrFI	CCNGG	2 cut(s)	669, 806
BmrI	ACTGGG	1 cut(s)	1015
BmsI	GCATC	5 cut(s)	275, 498, 934, 960, 1084
BmtI	GCTAGC	1 cut(s)	8
BmuI	ACTGGG	1 cut(s)	1015
BpiI	GAAGAC	1 cut(s)	1091
BplI	GAGNNNNNCTC	2 cut(s)	967, 999
BpmI	CTGGAG	1 cut(s)	1121
Bpu10I	CCTNAGC	1 cut(s)	974
BpuEI	CTTGAG	3 cut(s)	41, 585, 858
BsaAI	YACGTR	1 cut(s)	767
BsaBI	GATNNNNATC	3 cut(s)	15, 372, 520
BsaJI	CCNNGG	3 cut(s)	44, 667, 1079
BsaWI	WCCGGW	1 cut(s)	431
Bsc4I	CCNNNNNNNGG	7 cut(s)	107, 108, 242, 502, 506, 770, 1026
Bse1I	ACTGG	4 cut(s)	162, 879, 1021, 1104
Bse21I	CCTNAGG	1 cut(s)	89
Bse8I	GATNNNNATC	3 cut(s)	15, 372, 520
BseBI	CCWGG	2 cut(s)	669, 806
BseDI	CCNNGG	3 cut(s)	44, 667, 1079
BseGI	GGATG	9 cut(s)	158, 398, 489, 646, 814, 823, 856, 1016, 1088
BseJI	GATNNNNATC	3 cut(s)	15, 372, 520
BseLI	CCNNNNNNNGG	7 cut(s)	107, 108, 242, 502, 506, 770, 1026
BseMII	CTCAG	5 cut(s)	80, 750, 926, 965, 1123
BseNI	ACTGG	4 cut(s)	162, 879, 1021, 1104
BseRI	GAGGAG	4 cut(s)	159, 285, 1062, 1118
BseSI	GKGCMC	1 cut(s)	512
BseXI	GCAGC	1 cut(s)	934
BseYI	CCCAGC	1 cut(s)	418
Bsh1236I	CGCG	2 cut(s)	1081, 1159
Bsh1285I	CGRYCG	1 cut(s)	155
BshFI	GGCC	4 cut(s)	211, 510, 653, 895
BsiEI	CGRYCG	1 cut(s)	155
BsiHKAI	GWGCWC	2 cut(s)	151, 1110
BsiHKCI	CYCGRG	1 cut(s)	787
BsiSI	CCGG	2 cut(s)	432, 654
BslI	CCNNNNNNNGG	7 cut(s)	107, 108, 242, 502, 506, 770, 1026
BsmAI	GTCTC	2 cut(s)	612, 653
BsnI	GGCC	4 cut(s)	211, 510, 653, 895
BsoBI	CYCGRG	1 cut(s)	787
Bsp120I	GGGCCC	1 cut(s)	508
Bsp1286I	GDGCHC	3 cut(s)	151, 512, 1110
Bsp143I	GATC	4 cut(s)	245, 290, 538, 589
BspACI	CCGC	7 cut(s)	78, 155, 986, 1079, 1081, 1152, 1159
BspANI	GGCC	4 cut(s)	211, 510, 653, 895
BspCNI	CTCAG	5 cut(s)	81, 749, 925, 966, 1122
BspFNI	CGCG	2 cut(s)	1081, 1159
BspLI	GGNNCC	2 cut(s)	509, 510
BspMI	ACCTGC	1 cut(s)	262
BspOI	GCTAGC	1 cut(s)	8
BspPI	GGATC	3 cut(s)	253, 298, 533
BsrBI	CCGCTC	1 cut(s)	1152
BsrI	ACTGG	4 cut(s)	162, 879, 1021, 1104
BssECI	CCNNGG	3 cut(s)	44, 667, 1079
BssMI	GATC	4 cut(s)	245, 290, 538, 589
Bst2UI	CCWGG	2 cut(s)	669, 806
Bst6I	CTCTTC	1 cut(s)	561
BstAPI	GCANNNNNTGC	1 cut(s)	263
BstBAI	YACGTR	1 cut(s)	767
BstC8I	GCNNGC	2 cut(s)	6, 1167
BstDEI	CTNAG	7 cut(s)	89, 238, 341, 736, 912, 974, 1109
BstDSI	CCRYGG	1 cut(s)	1079
BstF5I	GGATG	9 cut(s)	158, 398, 489, 646, 814, 823, 856, 1016, 1088
BstFNI	CGCG	2 cut(s)	1081, 1159
BstHHI	GCGC	1 cut(s)	996
BstKTI	GATC	4 cut(s)	248, 293, 541, 592
BstMAI	GTCTC	2 cut(s)	612, 653
BstMBI	GATC	4 cut(s)	245, 290, 538, 589
BstMCI	CGRYCG	1 cut(s)	155
BstMWI	GCNNNNNNNGC	2 cut(s)	186, 263
BstNI	CCWGG	2 cut(s)	669, 806
BstSCI	CCNGG	2 cut(s)	667, 804
BstSFI	CTRYAG	1 cut(s)	331
BstSLI	GKGCMC	1 cut(s)	512
BstUI	CGCG	2 cut(s)	1081, 1159
BstV1I	GCAGC	1 cut(s)	934
BstV2I	GAAGAC	1 cut(s)	1091
BstX2I	RGATCY	2 cut(s)	245, 290
BstXI	CCANNNNNNTGG	1 cut(s)	189
BstYI	RGATCY	2 cut(s)	245, 290
Bsu36I	CCTNAGG	1 cut(s)	89
BsuRI	GGCC	4 cut(s)	211, 510, 653, 895
BtgI	CCRYGG	1 cut(s)	1079
BtrI	CACGTC	1 cut(s)	847
BtsCI	GGATG	9 cut(s)	158, 398, 489, 646, 814, 823, 856, 1016, 1088
BtsI	GCAGTG	1 cut(s)	917
BtsIMutI	CAGTG	4 cut(s)	169, 886, 917, 1097
BveI	ACCTGC	1 cut(s)	262
Cac8I	GCNNGC	2 cut(s)	6, 1167
CaiI	CAGNNNCTG	3 cut(s)	476, 919, 973
CfoI	GCGC	1 cut(s)	996
Cfr13I	GGNCC	3 cut(s)	508, 509, 651
Cfr42I	CCGCGG	1 cut(s)	1082
CviAII	CATG	3 cut(s)	281, 834, 1040
DdeI	CTNAG	7 cut(s)	89, 238, 341, 736, 912, 974, 1109
DpnI	GATC	4 cut(s)	247, 292, 540, 591
DpnII	GATC	4 cut(s)	245, 290, 538, 589
DraIII	CACNNNGTG	1 cut(s)	767
DrdI	GACNNNNNNGTC	1 cut(s)	342
DseDI	GACNNNNNNGTC	1 cut(s)	342
Eam1104I	CTCTTC	1 cut(s)	561
EarI	CTCTTC	1 cut(s)	561
EciI	GGCGGA	1 cut(s)	975
Ecl136II	GAGCTC	1 cut(s)	1108
Eco147I	AGGCCT	1 cut(s)	895
Eco24I	GRGCYC	2 cut(s)	512, 1110
Eco53kI	GAGCTC	1 cut(s)	1108
Eco57I	CTGAAG	2 cut(s)	12, 882
Eco72I	CACGTG	1 cut(s)	767
Eco81I	CCTNAGG	1 cut(s)	89
Eco88I	CYCGRG	1 cut(s)	787
EcoICRI	GAGCTC	1 cut(s)	1108
EcoO109I	RGGNCCY	2 cut(s)	508, 509
EcoRI	GAATTC	1 cut(s)	462
EcoRII	CCWGG	2 cut(s)	667, 804
EcoT38I	GRGCYC	2 cut(s)	512, 1110
FaeI	CATG	3 cut(s)	284, 837, 1043
FatI	CATG	3 cut(s)	280, 833, 1039
FauI	CCCGC	1 cut(s)	1152
FbaI	TGATCA	1 cut(s)	589
Fnu4HI	GCNGC	3 cut(s)	78, 948, 1079
FokI	GGATG	9 cut(s)	145, 385, 476, 653, 821, 830, 863, 1003, 1075
FriOI	GRGCYC	2 cut(s)	512, 1110
Fsp4HI	GCNGC	3 cut(s)	78, 948, 1079
FspBI	CTAG	4 cut(s)	5, 513, 753, 780
GlaI	GCGC	1 cut(s)	995
GluI	GCNGC	3 cut(s)	78, 948, 1079
GsaI	CCCAGC	1 cut(s)	422
GsuI	CTGGAG	1 cut(s)	1121
HaeIII	GGCC	4 cut(s)	211, 510, 653, 895
HapII	CCGG	2 cut(s)	432, 654
HhaI	GCGC	1 cut(s)	996
Hin1II	CATG	3 cut(s)	284, 837, 1043
Hin6I	GCGC	1 cut(s)	994
HinP1I	GCGC	1 cut(s)	994
HinfI	GANTC	7 cut(s)	605, 618, 661, 722, 869, 1001, 1178
HpaII	CCGG	2 cut(s)	432, 654
HphI	GGTGA	6 cut(s)	375, 405, 561, 656, 712, 753
Hpy188I	TCNGA	8 cut(s)	367, 538, 632, 696, 901, 969, 1093, 1112
Hpy188III	TCNNGA	7 cut(s)	20, 141, 471, 602, 615, 738, 780
Hpy99I	CGWCG	1 cut(s)	409
HpyAV	CCTTC	6 cut(s)	36, 106, 506, 691, 906, 1145
HpyCH4IV	ACGT	2 cut(s)	766, 846
HpyCH4V	TGCA	5 cut(s)	125, 257, 266, 454, 1097
HpyF10VI	GCNNNNNNNGC	2 cut(s)	186, 263
HpyF3I	CTNAG	7 cut(s)	89, 238, 341, 736, 912, 974, 1109
HpySE526I	ACGT	2 cut(s)	766, 846
Hsp92II	CATG	3 cut(s)	284, 837, 1043
HspAI	GCGC	1 cut(s)	994
Ksp22I	TGATCA	1 cut(s)	589
KspI	CCGCGG	1 cut(s)	1082
Kzo9I	GATC	4 cut(s)	245, 290, 538, 589
LmnI	GCTCC	3 cut(s)	146, 988, 1105
Lsp1109I	GCAGC	1 cut(s)	934
LweI	GCATC	5 cut(s)	275, 498, 934, 960, 1084
MaeI	CTAG	4 cut(s)	5, 513, 753, 780
MaeII	ACGT	2 cut(s)	766, 846
MaeIII	GTNAC	3 cut(s)	276, 915, 1010
MalI	GATC	4 cut(s)	247, 292, 540, 591
MbiI	CCGCTC	1 cut(s)	1152
MboI	GATC	4 cut(s)	245, 290, 538, 589
MboII	GAAGA	3 cut(s)	548, 1042, 1096
MflI	RGATCY	2 cut(s)	245, 290
MhlI	GDGCHC	3 cut(s)	151, 512, 1110
MluCI	AATT	4 cut(s)	51, 439, 462, 529
MlyI	GAGTC	2 cut(s)	614, 655
MmeI	TCCRAC	4 cut(s)	223, 268, 618, 751
MroXI	GAANNNNTTC	1 cut(s)	466
MspA1I	CMGCKG	3 cut(s)	80, 189, 1081
MspI	CCGG	2 cut(s)	432, 654
MspR9I	CCNGG	2 cut(s)	669, 806
MvaI	CCWGG	2 cut(s)	669, 806
MvnI	CGCG	2 cut(s)	1081, 1159
MwoI	GCNNNNNNNGC	2 cut(s)	186, 263
NdeII	GATC	4 cut(s)	245, 290, 538, 589
NheI	GCTAGC	1 cut(s)	4
NlaIII	CATG	3 cut(s)	284, 837, 1043
NlaIV	GGNNCC	2 cut(s)	509, 510
NmuCI	GTSAC	2 cut(s)	276, 915
PaeR7I	CTCGAG	1 cut(s)	787
PaqCI	CACCTGC	1 cut(s)	262
PceI	AGGCCT	1 cut(s)	895
PcsI	WCGNNNNNNNCGW	1 cut(s)	401
PdmI	GAANNNNTTC	1 cut(s)	466
PfeI	GAWTC	5 cut(s)	618, 722, 869, 1001, 1178
PflMI	CCANNNNNTGG	1 cut(s)	770
PfoI	TCCNGGA	1 cut(s)	804
PkrI	GCNGC	3 cut(s)	79, 949, 1080
PleI	GAGTC	2 cut(s)	613, 655
PmaCI	CACGTG	1 cut(s)	767
PmlI	CACGTG	1 cut(s)	767
PpsI	GAGTC	2 cut(s)	613, 655
Ppu21I	YACGTR	1 cut(s)	767
Psp124BI	GAGCTC	1 cut(s)	1110
Psp6I	CCWGG	2 cut(s)	667, 804
PspCI	CACGTG	1 cut(s)	767
PspFI	CCCAGC	1 cut(s)	418
PspGI	CCWGG	2 cut(s)	667, 804
PspN4I	GGNNCC	2 cut(s)	509, 510
PspOMI	GGGCCC	1 cut(s)	508
PspPI	GGNCC	3 cut(s)	508, 509, 651
PspXI	VCTCGAGB	1 cut(s)	787
PstNI	CAGNNNCTG	3 cut(s)	476, 919, 973
PsuI	RGATCY	2 cut(s)	245, 290
PvuII	CAGCTG	1 cut(s)	189
SacI	GAGCTC	1 cut(s)	1110
SacII	CCGCGG	1 cut(s)	1082
SatI	GCNGC	3 cut(s)	78, 948, 1079
Sau3AI	GATC	4 cut(s)	245, 290, 538, 589
Sau96I	GGNCC	3 cut(s)	508, 509, 651
SchI	GAGTC	2 cut(s)	614, 655
ScrFI	CCNGG	2 cut(s)	669, 806
SduI	GDGCHC	3 cut(s)	151, 512, 1110
SfaNI	GCATC	5 cut(s)	275, 498, 934, 960, 1084
SfcI	CTRYAG	1 cut(s)	331
Sfr274I	CTCGAG	1 cut(s)	787
Sfr303I	CCGCGG	1 cut(s)	1082
SgrBI	CCGCGG	1 cut(s)	1082
SlaI	CTCGAG	1 cut(s)	787
SmlI	CTYRAG	4 cut(s)	20, 600, 787, 873
SmoI	CTYRAG	4 cut(s)	20, 600, 787, 873
Sse9I	AATT	4 cut(s)	51, 439, 462, 529
SseBI	AGGCCT	1 cut(s)	895
SsiI	CCGC	7 cut(s)	78, 155, 986, 1079, 1081, 1152, 1159
SspMI	CTAG	4 cut(s)	5, 513, 753, 780
SstI	GAGCTC	1 cut(s)	1110
StuI	AGGCCT	1 cut(s)	895
StyD4I	CCNGG	2 cut(s)	667, 804
TaiI	ACGT	2 cut(s)	769, 849
TaqI	TCGA	3 cut(s)	140, 460, 788
TasI	AATT	4 cut(s)	51, 439, 462, 529
TauI	GCSGC	2 cut(s)	80, 1081
TfiI	GAWTC	5 cut(s)	618, 722, 869, 1001, 1178
TscAI	CASTG	4 cut(s)	169, 886, 924, 1104
TseFI	GTSAC	2 cut(s)	276, 915
TseI	GCWGC	1 cut(s)	947
Tsp45I	GTSAC	2 cut(s)	276, 915
TspDTI	ATGAA	3 cut(s)	719, 895, 1142
TspGWI	ACGGA	1 cut(s)	828
TspRI	CASTG	4 cut(s)	169, 886, 924, 1104
Van91I	CCANNNNNTGG	1 cut(s)	770
XapI	RAATTY	1 cut(s)	462
XbaI	TCTAGA	1 cut(s)	779
XhoI	CTCGAG	1 cut(s)	787
XmnI	GAANNNNTTC	1 cut(s)	466
XspI	CTAG	4 cut(s)	5, 513, 753, 780

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.