pycom08g00440

PRONE (Plant-specific Rop nucleotide exchanger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0004730
Physical Location & Seq
Forward (+)
412132 .. 414770
2639 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1449 bp
ATGGGGAGCATCGGCGTCTCGTCCGAGGATGATGAGTTGGAGCAGTTGAGCGAGCGCTTCGACAGTTACAGCTTGAGTGCCGACGTCAGTGAATCCGAAAGCTCCAGTGGCTTTTCCTGTCGACTGTACGGTCGCCTAGCTCCTGCTGCTTCCACTTCCCTCACTTCTTCGCCTCCATCTGGACCCGACTTCGCTGACATTTCTTATTCTCAGCAGCCGCCCATGCCGTTTGTTTTGCCGGCGGTAGGTGGACGACGTGTCGACCTTCCGCCGACCATGACGGAGAAACCCCATACCAAGTTGTCCGAAGTTGAATTGATGAAGGAGCGGTTTGCAAAGCTTCTGCTCGGAGAAGATATGTCCGGCGGAGGCAAGGGAGTCTGTACTGCGCTTGCTATTTCCAATGCAATCACAAATCTTTCTGCCACCGTATTTGGGGAGTTGTGGAAGTTGGAGCCTCTGGCTCCCCAGAAGAAGGCAATGTGGTGCCGAGAGATGGATTGGCTTTTGTCTGTAAGTGATTCTATTGTTGAGCTCATACCATCAGTACAAGATTTCCCTGGTGGTGGGACCTTTGAGGTTATGGTCACTCGGCCGCGCTCTGATCTGTATGTAAACCTCCCGGCACTCAAGAAACTGGATGCAATGCTGCTTAGCATTCTTGACGGATTCCACGACTCTGAGTTTTACTATGTTGACCGAGGAATAATTGTTCCTGATTCTGATGATGGTGCCTTGTCTTCCTCTTCCGGCAGACCATCAACTAGGCAAGAAGAGAAGTGGTGGCTCCCATTCCCTAAGGTCCCCTCAAATGGTCTGTCGGAAAATGTGAGAAAGACCTTGCAACAGTGTCGGGATTGCACAAATCAGATCCTTAAGGCTGCAATGGCCATCAATAGTAGTGTGCTAGCTGAAATGGAAACCCCAGATGCTTACTTGGAGAGTTTGCCCAAGAGTGGGAAAGCTTGTTTAGGCGAAATCATTTACCGTTTTATAGCTGCTGATCGGTTTTCCCCAGAAGGTCTTCTTGATTACTTGGACCTCTCATCAGAATACACCACTCTAGAAATAGCAAACAGGATTGAGGCTGCCGCACACATTTGGAAGCAGAAGCATGTGAAACCCCATCTAACTCGTGTAAATTCTGCAAAGTCATCCTGGGTTGGAAAGGTCAAAGGCTTTGTCAGTGAAATAGAAAAGAGTAAGCTTCTCGCATATAGGACGGATACCCTCTTACAGAATCTGAAGATACGGTTCCCTGGCCTCCCACAGACTGCTCTGGACATGAACAAGATTCAGTACAACAGGGATGTGGGGCAATCAATCCTTGAGAGCTACTCTAGGGTGATGGAAAGCATAGCCTTCAACATAATGGCAAGGATAGATGATCTACTATACGTAGATGATGCCACCAAACAGCGGGCAGCTGCAGAGTCTATGTCTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRONE

Protein Analysis

483

Amino Acids

53.16

Weight (kDa)

5.1

Isoelectric Point (pI)

51.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRONE PF03759 102 - 472 1.8e-175 PRONE (Plant-specific Rop nucleotide exchanger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 129
AatII GACGTC 1 cut(s) 87
AccB1I GGYRCC 2 cut(s) 486, 731
AccBSI CCGCTC 1 cut(s) 328
AccI GTMKAC 2 cut(s) 121, 261
AccII CGCG 1 cut(s) 598
AciI CCGC 8 cut(s) 218, 242, 269, 328, 366, 596, 1092, 1420
AclWI GGATC 1 cut(s) 865
AcoI YGGCCR 2 cut(s) 593, 888
AcsI RAATTY 1 cut(s) 1141
AcuI CTGAAG 1 cut(s) 1265
AcyI GRCGYC 2 cut(s) 15, 84
AfaI GTAC 4 cut(s) 128, 385, 549, 1301
AfeI AGCGCT 1 cut(s) 56
AflII CTTAAG 1 cut(s) 875
AflIII ACRYGT 1 cut(s) 256
AgsI TTSAA 2 cut(s) 314, 1366
AhdI GACNNNNNGTC 1 cut(s) 257
AjiI CACGTC 1 cut(s) 257
AjnI CCWGG 3 cut(s) 559, 1157, 1258
AloI GAACNNNNNNTCC 2 cut(s) 696, 728
Alw21I GWGCWC 1 cut(s) 537
Alw26I GTCTC 1 cut(s) 22
AlwI GGATC 1 cut(s) 865
AlwNI CAGNNNCTG 1 cut(s) 1243
Aor51HI AGCGCT 1 cut(s) 56
AoxI GGCC 3 cut(s) 593, 888, 1261
ApeKI GCWGC 8 cut(s) 146, 214, 649, 881, 998, 1088, 1424, 1427
ApoI RAATTY 1 cut(s) 1141
ArsI GACNNNNNNTTYG 4 cut(s) 803, 835, 1167, 1199
Asp700I GAANNNNTTC 1 cut(s) 1023
AspLEI GCGC 3 cut(s) 57, 391, 600
AspS9I GGNCC 4 cut(s) 182, 570, 802, 1039
AsuC2I CCSGG 1 cut(s) 623
AsuHPI GGTGA 1 cut(s) 1357
AsuNHI GCTAGC 1 cut(s) 907
AvaII GGWCC 4 cut(s) 182, 570, 802, 1039
AxyI CCTNAGG 1 cut(s) 798
BalI TGGCCA 1 cut(s) 890
BanI GGYRCC 2 cut(s) 486, 731
BanII GRGCYC 1 cut(s) 537
BauI CACGAG 1 cut(s) 1134
BbsI GAAGAC 2 cut(s) 732, 1016
Bbv12I GWGCWC 1 cut(s) 537
BbvI GCAGC 8 cut(s) 133, 226, 636, 868, 985, 1075, 1414, 1436
BccI CCATC 8 cut(s) 184, 490, 550, 722, 766, 899, 1134, 1342
BceAI ACGGC 1 cut(s) 211
BcgI CGANNNNNNTGC 2 cut(s) 833, 867
BciT130I CCWGG 3 cut(s) 561, 1159, 1260
BciVI GTATCC 1 cut(s) 1219
BcnI CCSGG 1 cut(s) 623
BcoDI GTCTC 1 cut(s) 22
BfaI CTAG 5 cut(s) 137, 765, 908, 1064, 1341
BfmI CTRYAG 1 cut(s) 1428
BfoI RGCGCY 1 cut(s) 58
BfrI CTTAAG 1 cut(s) 875
BfuI GTATCC 1 cut(s) 1219
BlpI GCTNAGC 1 cut(s) 653
Bme1390I CCNGG 4 cut(s) 561, 623, 1159, 1260
Bme18I GGWCC 4 cut(s) 182, 570, 802, 1039
BmeRI GACNNNNNGTC 1 cut(s) 257
BmgBI CACGTC 1 cut(s) 257
BmgT120I GGNCC 4 cut(s) 182, 570, 802, 1039
BmiI GGNNCC 9 cut(s) 184, 456, 465, 488, 571, 733, 788, 804, 1256
BmrFI CCNGG 4 cut(s) 561, 623, 1159, 1260
BmsI GCATC 4 cut(s) 18, 631, 919, 1396
BmtI GCTAGC 1 cut(s) 911
BpiI GAAGAC 2 cut(s) 732, 1016
BplI GAGNNNNNCTC 2 cut(s) 1322, 1354
BpmI CTGGAG 1 cut(s) 88
Bpu1102I GCTNAGC 1 cut(s) 653
BpuEI CTTGAG 3 cut(s) 94, 614, 1349
BpuMI CCSGG 1 cut(s) 623
BsaAI YACGTR 1 cut(s) 1399
BsaHI GRCGYC 2 cut(s) 15, 84
BsaJI CCNNGG 5 cut(s) 24, 559, 700, 1158, 1258
Bse118I RCCGGY 1 cut(s) 238
Bse1I ACTGG 2 cut(s) 105, 642
Bse21I CCTNAGG 1 cut(s) 798
Bse3DI GCAATG 3 cut(s) 486, 651, 891
BseBI CCWGG 3 cut(s) 561, 1159, 1260
BseDI CCNNGG 5 cut(s) 24, 559, 700, 1158, 1258
BseGI GGATG 4 cut(s) 34, 646, 1154, 1315
BseMI GCAATG 3 cut(s) 486, 651, 891
BseMII CTCAG 2 cut(s) 224, 672
BseNI ACTGG 2 cut(s) 105, 642
BseX3I CGGCCG 1 cut(s) 593
BseXI GCAGC 8 cut(s) 133, 226, 636, 868, 985, 1075, 1414, 1436
Bsh1236I CGCG 1 cut(s) 598
Bsh1285I CGRYCG 2 cut(s) 133, 596
BshFI GGCC 3 cut(s) 595, 890, 1263
BshNI GGYRCC 2 cut(s) 486, 731
BsiEI CGRYCG 2 cut(s) 133, 596
BsiHKAI GWGCWC 1 cut(s) 537
BsiSI CCGG 4 cut(s) 239, 363, 623, 750
BslFI GGGAC 2 cut(s) 583, 788
BsmAI GTCTC 1 cut(s) 22
BsmBI CGTCTC 1 cut(s) 22
BsmFI GGGAC 2 cut(s) 583, 788
BsmI GAATGC 1 cut(s) 657
BsnI GGCC 3 cut(s) 595, 890, 1263
Bsp1286I GDGCHC 1 cut(s) 537
Bsp143I GATC 4 cut(s) 604, 870, 1003, 1387
Bsp1720I GCTNAGC 1 cut(s) 653
BspACI CCGC 8 cut(s) 218, 242, 269, 328, 366, 596, 1092, 1420
BspANI GGCC 3 cut(s) 595, 890, 1263
BspCNI CTCAG 2 cut(s) 223, 673
BspFNI CGCG 1 cut(s) 598
BspLI GGNNCC 9 cut(s) 184, 456, 465, 488, 571, 733, 788, 804, 1256
BspMAI CTGCAG 1 cut(s) 1432
BspOI GCTAGC 1 cut(s) 911
BspPI GGATC 1 cut(s) 865
BspT107I GGYRCC 2 cut(s) 486, 731
BspTI CTTAAG 1 cut(s) 875
BsrBI CCGCTC 1 cut(s) 328
BsrDI GCAATG 3 cut(s) 486, 651, 891
BsrFI RCCGGY 1 cut(s) 238
BsrI ACTGG 2 cut(s) 105, 642
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 5 cut(s) 24, 559, 700, 1158, 1258
BssMI GATC 4 cut(s) 604, 870, 1003, 1387
BssNI GRCGYC 2 cut(s) 15, 84
BssSI CACGAG 1 cut(s) 1134
Bst2BI CACGAG 1 cut(s) 1134
Bst2UI CCWGG 3 cut(s) 561, 1159, 1260
Bst4CI ACNGT 7 cut(s) 65, 126, 131, 430, 849, 989, 1254
Bst6I CTCTTC 2 cut(s) 751, 768
BstACI GRCGYC 2 cut(s) 15, 84
BstAFI CTTAAG 1 cut(s) 875
BstBAI YACGTR 1 cut(s) 1399
BstC8I GCNNGC 5 cut(s) 53, 240, 393, 909, 1422
BstDEI CTNAG 4 cut(s) 210, 653, 681, 798
BstF5I GGATG 4 cut(s) 34, 646, 1154, 1315
BstFNI CGCG 1 cut(s) 598
BstH2I RGCGCY 1 cut(s) 58
BstHHI GCGC 3 cut(s) 57, 391, 600
BstKTI GATC 4 cut(s) 607, 873, 1006, 1390
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 4 cut(s) 604, 870, 1003, 1387
BstMCI CGRYCG 2 cut(s) 133, 596
BstMWI GCNNNNNNNGC 4 cut(s) 108, 146, 223, 887
BstNI CCWGG 3 cut(s) 561, 1159, 1260
BstNSI RCATGY 1 cut(s) 1118
BstSCI CCNGG 4 cut(s) 559, 621, 1157, 1258
BstSFI CTRYAG 1 cut(s) 1428
BstSNI TACGTA 1 cut(s) 1399
BstUI CGCG 1 cut(s) 598
BstV1I GCAGC 8 cut(s) 133, 226, 636, 868, 985, 1075, 1414, 1436
BstV2I GAAGAC 2 cut(s) 732, 1016
BstX2I RGATCY 1 cut(s) 870
BstYI RGATCY 1 cut(s) 870
BstZI CGGCCG 1 cut(s) 593
Bsu36I CCTNAGG 1 cut(s) 798
BsuI GTATCC 1 cut(s) 1219
BsuRI GGCC 3 cut(s) 595, 890, 1263
BtrI CACGTC 1 cut(s) 257
BtsCI GGATG 4 cut(s) 34, 646, 1154, 1315
BtsIMutI CAGTG 4 cut(s) 94, 112, 854, 1192
Cac8I GCNNGC 5 cut(s) 53, 240, 393, 909, 1422
CaiI CAGNNNCTG 1 cut(s) 1243
CfoI GCGC 3 cut(s) 57, 391, 600
Cfr10I RCCGGY 1 cut(s) 238
Cfr13I GGNCC 4 cut(s) 182, 570, 802, 1039
CseI GACGC 1 cut(s) 4
Csp6I GTAC 4 cut(s) 127, 384, 548, 1300
CspCI CAANNNNNGTGG 2 cut(s) 415, 450
CviAII CATG 4 cut(s) 223, 277, 1115, 1285
CviQI GTAC 4 cut(s) 127, 384, 548, 1300
DdeI CTNAG 4 cut(s) 210, 653, 681, 798
DpnI GATC 4 cut(s) 606, 872, 1005, 1389
DpnII GATC 4 cut(s) 604, 870, 1003, 1387
DrdI GACNNNNNNGTC 1 cut(s) 129
DriI GACNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 129
EaeI YGGCCR 2 cut(s) 593, 888
EagI CGGCCG 1 cut(s) 593
Eam1104I CTCTTC 2 cut(s) 751, 768
Eam1105I GACNNNNNGTC 1 cut(s) 257
EarI CTCTTC 2 cut(s) 751, 768
EciI GGCGGA 2 cut(s) 258, 381
Ecl136II GAGCTC 1 cut(s) 535
EclXI CGGCCG 1 cut(s) 593
Eco105I TACGTA 1 cut(s) 1399
Eco24I GRGCYC 1 cut(s) 537
Eco47I GGWCC 4 cut(s) 182, 570, 802, 1039
Eco47III AGCGCT 1 cut(s) 56
Eco52I CGGCCG 1 cut(s) 593
Eco53kI GAGCTC 1 cut(s) 535
Eco57I CTGAAG 1 cut(s) 1265
Eco81I CCTNAGG 1 cut(s) 798
EcoICRI GAGCTC 1 cut(s) 535
EcoO109I RGGNCCY 2 cut(s) 570, 802
EcoRII CCWGG 3 cut(s) 559, 1157, 1258
EcoT38I GRGCYC 1 cut(s) 537
Esp3I CGTCTC 1 cut(s) 22
FaeI CATG 4 cut(s) 226, 280, 1118, 1288
FalI AAGNNNNNCTT 2 cut(s) 1011, 1043
FaqI GGGAC 2 cut(s) 583, 788
FatI CATG 4 cut(s) 222, 276, 1114, 1284
FauI CCCGC 1 cut(s) 1413
FblI GTMKAC 2 cut(s) 121, 261
FokI GGATG 4 cut(s) 41, 653, 1141, 1322
FriOI GRGCYC 1 cut(s) 537
FspBI CTAG 5 cut(s) 137, 765, 908, 1064, 1341
GlaI GCGC 3 cut(s) 56, 390, 599
GsuI CTGGAG 1 cut(s) 88
HaeII RGCGCY 1 cut(s) 58
HaeIII GGCC 3 cut(s) 595, 890, 1263
HapII CCGG 4 cut(s) 239, 363, 623, 750
HgaI GACGC 1 cut(s) 4
HhaI GCGC 3 cut(s) 57, 391, 600
Hin1I GRCGYC 2 cut(s) 15, 84
Hin1II CATG 4 cut(s) 226, 280, 1118, 1288
Hin6I GCGC 3 cut(s) 55, 389, 598
HinP1I GCGC 3 cut(s) 55, 389, 598
HincII GTYRAC 3 cut(s) 122, 262, 697
HindII GTYRAC 3 cut(s) 122, 262, 697
HindIII AAGCTT 3 cut(s) 338, 963, 1205
HinfI GANTC 9 cut(s) 92, 378, 521, 669, 677, 719, 1240, 1294, 1433
HpaII CCGG 4 cut(s) 239, 363, 623, 750
HphI GGTGA 1 cut(s) 1357
Hpy166II GTNNAC 5 cut(s) 122, 251, 262, 616, 697
Hpy188III TCNNGA 9 cut(s) 180, 631, 662, 716, 854, 1028, 1064, 1280, 1443
Hpy8I GTNNAC 5 cut(s) 122, 251, 262, 616, 697
Hpy99I CGWCG 2 cut(s) 86, 258
HpyAV CCTTC 5 cut(s) 275, 316, 469, 1013, 1372
HpyCH4III ACNGT 7 cut(s) 65, 126, 131, 430, 849, 989, 1254
HpyCH4IV ACGT 3 cut(s) 84, 256, 1398
HpyCH4V TGCA 8 cut(s) 335, 407, 644, 844, 861, 884, 1148, 1430
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 146, 223, 887
HpyF3I CTNAG 4 cut(s) 210, 653, 681, 798
HpySE526I ACGT 3 cut(s) 84, 256, 1398
Hsp92I GRCGYC 2 cut(s) 15, 84
Hsp92II CATG 4 cut(s) 226, 280, 1118, 1288
HspAI GCGC 3 cut(s) 55, 389, 598
KroI GCCGGC 1 cut(s) 238
KroNI GCCGGC 1 cut(s) 240
Kzo9I GATC 4 cut(s) 604, 870, 1003, 1387
LmnI GCTCC 8 cut(s) 6, 40, 107, 145, 325, 454, 469, 792
Lsp1109I GCAGC 8 cut(s) 133, 226, 636, 868, 985, 1075, 1414, 1436
LweI GCATC 4 cut(s) 18, 631, 919, 1396
MaeI CTAG 5 cut(s) 137, 765, 908, 1064, 1341
MaeII ACGT 3 cut(s) 84, 256, 1398
MaeIII GTNAC 2 cut(s) 65, 586
MalI GATC 4 cut(s) 606, 872, 1005, 1389
MbiI CCGCTC 1 cut(s) 328
MboI GATC 4 cut(s) 604, 870, 1003, 1387
MboII GAAGA 8 cut(s) 159, 365, 484, 732, 738, 785, 1016, 1258
MflI RGATCY 1 cut(s) 870
MhlI GDGCHC 1 cut(s) 537
MlsI TGGCCA 1 cut(s) 890
MluCI AATT 3 cut(s) 314, 708, 1141
MluNI TGGCCA 1 cut(s) 890
MlyI GAGTC 3 cut(s) 387, 671, 1442
MmeI TCCRAC 4 cut(s) 18, 432, 801, 1144
Mox20I TGGCCA 1 cut(s) 890
MroNI GCCGGC 1 cut(s) 238
MroXI GAANNNNTTC 1 cut(s) 1023
MscI TGGCCA 1 cut(s) 890
MseI TTAA 1 cut(s) 876
Msp20I TGGCCA 1 cut(s) 890
MspA1I CMGCKG 2 cut(s) 1420, 1427
MspCI CTTAAG 1 cut(s) 875
MspI CCGG 4 cut(s) 239, 363, 623, 750
MspR9I CCNGG 4 cut(s) 561, 623, 1159, 1260
Mva1269I GAATGC 1 cut(s) 657
MvaI CCWGG 3 cut(s) 561, 1159, 1260
MvnI CGCG 1 cut(s) 598
MwoI GCNNNNNNNGC 4 cut(s) 108, 146, 223, 887
NaeI GCCGGC 1 cut(s) 240
NciI CCSGG 1 cut(s) 623
NdeII GATC 4 cut(s) 604, 870, 1003, 1387
NgoMIV GCCGGC 1 cut(s) 238
NheI GCTAGC 1 cut(s) 907
NlaIII CATG 4 cut(s) 226, 280, 1118, 1288
NlaIV GGNNCC 9 cut(s) 184, 456, 465, 488, 571, 733, 788, 804, 1256
NmeAIII GCCGAG 2 cut(s) 515, 571
NmuCI GTSAC 1 cut(s) 586
NspI RCATGY 1 cut(s) 1118
PcsI WCGNNNNNNNCGW 1 cut(s) 672
PctI GAATGC 1 cut(s) 657
PdiI GCCGGC 1 cut(s) 240
PdmI GAANNNNTTC 1 cut(s) 1023
PfeI GAWTC 6 cut(s) 92, 521, 669, 719, 1240, 1294
PleI GAGTC 3 cut(s) 386, 671, 1441
PpsI GAGTC 3 cut(s) 386, 671, 1441
Ppu21I YACGTR 1 cut(s) 1399
PpuMI RGGWCCY 2 cut(s) 570, 802
Psp124BI GAGCTC 1 cut(s) 537
Psp5II RGGWCCY 2 cut(s) 570, 802
Psp6I CCWGG 3 cut(s) 559, 1157, 1258
PspGI CCWGG 3 cut(s) 559, 1157, 1258
PspN4I GGNNCC 9 cut(s) 184, 456, 465, 488, 571, 733, 788, 804, 1256
PspPI GGNCC 4 cut(s) 182, 570, 802, 1039
PspPPI RGGWCCY 2 cut(s) 570, 802
PstI CTGCAG 1 cut(s) 1432
PstNI CAGNNNCTG 1 cut(s) 1243
PsuI RGATCY 1 cut(s) 870
PvuII CAGCTG 1 cut(s) 1427
RsaI GTAC 4 cut(s) 128, 385, 549, 1301
RsaNI GTAC 4 cut(s) 127, 384, 548, 1300
SacI GAGCTC 1 cut(s) 537
SalI GTCGAC 2 cut(s) 120, 260
SaqAI TTAA 1 cut(s) 876
Sau3AI GATC 4 cut(s) 604, 870, 1003, 1387
Sau96I GGNCC 4 cut(s) 182, 570, 802, 1039
SchI GAGTC 3 cut(s) 387, 671, 1442
ScrFI CCNGG 4 cut(s) 561, 623, 1159, 1260
SduI GDGCHC 1 cut(s) 537
SfaNI GCATC 4 cut(s) 18, 631, 919, 1396
SfcI CTRYAG 1 cut(s) 1428
SinI GGWCC 4 cut(s) 182, 570, 802, 1039
SmlI CTYRAG 4 cut(s) 73, 629, 875, 1328
SmoI CTYRAG 4 cut(s) 73, 629, 875, 1328
SnaBI TACGTA 1 cut(s) 1399
Sse9I AATT 3 cut(s) 314, 708, 1141
SsiI CCGC 8 cut(s) 218, 242, 269, 328, 366, 596, 1092, 1420
SspMI CTAG 5 cut(s) 137, 765, 908, 1064, 1341
SstI GAGCTC 1 cut(s) 537
StyD4I CCNGG 4 cut(s) 559, 621, 1157, 1258
TaaI ACNGT 7 cut(s) 65, 126, 131, 430, 849, 989, 1254
TaiI ACGT 3 cut(s) 87, 259, 1401
TaqI TCGA 3 cut(s) 60, 121, 261
TaqII GACCGA 1 cut(s) 714
TasI AATT 3 cut(s) 314, 708, 1141
TatI WGTACW 3 cut(s) 383, 547, 1299
TauI GCSGC 3 cut(s) 220, 598, 1094
TfiI GAWTC 6 cut(s) 92, 521, 669, 719, 1240, 1294
Tru1I TTAA 1 cut(s) 876
Tru9I TTAA 1 cut(s) 876
TscAI CASTG 4 cut(s) 94, 112, 854, 1192
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 8 cut(s) 146, 214, 649, 881, 998, 1088, 1424, 1427
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 2 cut(s) 335, 1301
TspGWI ACGGA 3 cut(s) 296, 681, 1238
TspRI CASTG 4 cut(s) 94, 112, 854, 1192
Vha464I CTTAAG 1 cut(s) 875
VpaK11BI GGWCC 4 cut(s) 182, 570, 802, 1039
XapI RAATTY 1 cut(s) 1141
XbaI TCTAGA 1 cut(s) 1063
XceI RCATGY 1 cut(s) 1118
XmiI GTMKAC 2 cut(s) 121, 261
XmnI GAANNNNTTC 1 cut(s) 1023
XspI CTAG 5 cut(s) 137, 765, 908, 1064, 1341
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.