pycom08g01060

Chaperone protein dnaJ 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0017105
Physical Location & Seq
Reverse (-)
828386 .. 829054
669 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g01060.1

Sequence Viewer

Length: 531 bp
ATGGACACCGACGCCCCGCTGAGTCAGACACTCTCCCAAATGGATAAGATCCGACTCATACCAATCAAAATTTCGAAGCAAACAACAACAATCTCAAAAATGGCTTCCATATCCTCCTCATCTTTCACTTGCCACTCCTCTCCTCTTTTCTCCGTCGGCAACGACAGCCCCCGCGCTCCTCCCTCCTCCGTCAGCTACCGGCCCCTCCGCGTCTCCGCCTCGTGCGCATCGACGGTCGAAAGGCCGCGACCCCGCATTGGATCAAGCTCCTCACTGTACGACGTGCTCGGGATTCACGCCGGCGCCACGTGCCAGGAGATCAAGTCGGCGTACCGCAAGTTAGCGAGAGTTTTGCACCCGGACGTTGCGAAGTCATCCGCGGACGAGTTCATGAAAGTCCACGAAGCTTACTCCACTCTCTCCGACACGCAAAAACGCGCCGATTACGACCGCAAGATGATCGATCGGACGGCCGAGGACGGGCGTGGCTTCATCTTACGCTTACGCAACAACAATGTCGTCGATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

177

Amino Acids

19.37

Weight (kDa)

9.37

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 92 - 150 1.3e-20 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g38340
prunus_persica Prupe.1G364400_v2.0.a1
pyrus_communis pycom08g01060 pycom15g01000
rosa_chinensis RchiOBHm_Chr6g0303011
rosa_laevigata RLG00000011118
rosa_multiflora Rmu_ssc0000460.1_g000030
rosa_roxburghii Rroxscaffold_7G00165110
rosa_rugosa Rorug06G0318400
rosa_samantha Rh6AG432200 Rh6BG470300 Rh6DG431400
rosa_wichuraiana Rw6G037470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 226
AccB1I GGYRCC 1 cut(s) 302
AccII CGCG 5 cut(s) 174, 210, 247, 380, 438
AclWI GGATC 2 cut(s) 43, 268
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 1 cut(s) 69
AcvI CACGTG 1 cut(s) 309
AcyI GRCGYC 2 cut(s) 12, 303
AfaI GTAC 2 cut(s) 278, 332
AfiI CCNNNNNNNGG 2 cut(s) 257, 480
AjiI CACGTC 1 cut(s) 283
AjnI CCWGG 1 cut(s) 312
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AluBI AGCT 3 cut(s) 195, 267, 407
AluI AGCT 3 cut(s) 195, 267, 407
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 1 cut(s) 217
AlwI GGATC 2 cut(s) 43, 268
Ama87I CYCGRG 1 cut(s) 287
AoxI GGCC 3 cut(s) 200, 242, 471
ApoI RAATTY 1 cut(s) 69
AspLEI GCGC 4 cut(s) 176, 227, 305, 440
AspS9I GGNCC 1 cut(s) 201
AsuC2I CCSGG 1 cut(s) 359
AsuII TTCGAA 1 cut(s) 74
AvaI CYCGRG 1 cut(s) 287
BanI GGYRCC 1 cut(s) 302
BauI CACGAG 1 cut(s) 220
BbrPI CACGTG 1 cut(s) 309
Bbv12I GWGCWC 1 cut(s) 288
BceAI ACGGC 1 cut(s) 486
BcgI CGANNNNNNTGC 4 cut(s) 334, 368, 442, 476
BciT130I CCWGG 1 cut(s) 314
BcnI CCSGG 1 cut(s) 359
BcoDI GTCTC 1 cut(s) 217
BfaI CTAG 1 cut(s) 529
BfoI RGCGCY 1 cut(s) 306
BisI GCNGC 1 cut(s) 245
BlsI GCNGC 1 cut(s) 246
Bme1390I CCNGG 2 cut(s) 314, 359
BmeT110I CYCGRG 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 201
BmiI GGNNCC 2 cut(s) 203, 304
BmrFI CCNGG 2 cut(s) 314, 359
BmsI GCATC 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 74
BpuMI CCSGG 1 cut(s) 359
Bsa29I ATCGAT 1 cut(s) 462
BsaAI YACGTR 1 cut(s) 309
BsaHI GRCGYC 2 cut(s) 12, 303
BsaJI CCNNGG 2 cut(s) 378, 474
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 480
Bse118I RCCGGY 2 cut(s) 198, 299
BseBI CCWGG 1 cut(s) 314
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 2 cut(s) 378, 474
BseGI GGATG 1 cut(s) 374
BseLI CCNNNNNNNGG 2 cut(s) 257, 480
BseMII CTCAG 1 cut(s) 11
BseRI GAGGAG 6 cut(s) 106, 127, 132, 168, 175, 259
BseX3I CGGCCG 1 cut(s) 471
Bsh1236I CGCG 5 cut(s) 174, 210, 247, 380, 438
Bsh1285I CGRYCG 4 cut(s) 237, 451, 466, 474
BshFI GGCC 3 cut(s) 202, 244, 473
BshNI GGYRCC 1 cut(s) 302
BshVI ATCGAT 1 cut(s) 462
BsiEI CGRYCG 4 cut(s) 237, 451, 466, 474
BsiHKAI GWGCWC 1 cut(s) 288
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 3 cut(s) 199, 300, 359
BslI CCNNNNNNNGG 2 cut(s) 257, 480
BsmAI GTCTC 1 cut(s) 217
BsmBI CGTCTC 1 cut(s) 217
BsnI GGCC 3 cut(s) 202, 244, 473
BsoBI CYCGRG 1 cut(s) 287
Bsp119I TTCGAA 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 288
Bsp143I GATC 5 cut(s) 48, 260, 318, 459, 463
BspANI GGCC 3 cut(s) 202, 244, 473
BspCNI CTCAG 1 cut(s) 12
BspDI ATCGAT 1 cut(s) 462
BspFNI CGCG 5 cut(s) 174, 210, 247, 380, 438
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 2 cut(s) 203, 304
BspPI GGATC 2 cut(s) 43, 268
BspT104I TTCGAA 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 302
BsrFI RCCGGY 2 cut(s) 198, 299
BssAI RCCGGY 2 cut(s) 198, 299
BssECI CCNNGG 2 cut(s) 378, 474
BssMI GATC 5 cut(s) 48, 260, 318, 459, 463
BssNI GRCGYC 2 cut(s) 12, 303
BssSI CACGAG 1 cut(s) 220
Bst2BI CACGAG 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 2 cut(s) 235, 276
BstACI GRCGYC 2 cut(s) 12, 303
BstBAI YACGTR 1 cut(s) 309
BstBI TTCGAA 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 301
BstDEI CTNAG 1 cut(s) 20
BstDSI CCRYGG 1 cut(s) 378
BstF5I GGATG 1 cut(s) 374
BstFNI CGCG 5 cut(s) 174, 210, 247, 380, 438
BstH2I RGCGCY 1 cut(s) 306
BstHHI GCGC 4 cut(s) 176, 227, 305, 440
BstKTI GATC 5 cut(s) 51, 263, 321, 462, 466
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 5 cut(s) 48, 260, 318, 459, 463
BstMCI CGRYCG 4 cut(s) 237, 451, 466, 474
BstMWI GCNNNNNNNGC 3 cut(s) 165, 224, 309
BstNI CCWGG 1 cut(s) 314
BstSCI CCNGG 2 cut(s) 312, 357
BstUI CGCG 5 cut(s) 174, 210, 247, 380, 438
BstX2I RGATCY 1 cut(s) 48
BstYI RGATCY 1 cut(s) 48
BstZI CGGCCG 1 cut(s) 471
Bsu15I ATCGAT 1 cut(s) 462
BsuRI GGCC 3 cut(s) 202, 244, 473
BsuTUI ATCGAT 1 cut(s) 462
BtgI CCRYGG 1 cut(s) 378
BtrI CACGTC 1 cut(s) 283
BtsCI GGATG 1 cut(s) 374
BtsIMutI CAGTG 1 cut(s) 272
Cac8I GCNNGC 1 cut(s) 301
CciI TCATGA 1 cut(s) 390
CfoI GCGC 4 cut(s) 176, 227, 305, 440
Cfr10I RCCGGY 2 cut(s) 198, 299
Cfr13I GGNCC 1 cut(s) 201
Cfr42I CCGCGG 1 cut(s) 381
ClaI ATCGAT 1 cut(s) 462
CseI GACGC 2 cut(s) 20, 199
Csp6I GTAC 2 cut(s) 277, 331
CviAII CATG 1 cut(s) 391
CviJI RGCY 9 cut(s) 104, 168, 195, 202, 244, 267, 407, 473, 489
CviKI_1 RGCY 9 cut(s) 104, 168, 195, 202, 244, 267, 407, 473, 489
CviQI GTAC 2 cut(s) 277, 331
DdeI CTNAG 1 cut(s) 20
DinI GGCGCC 1 cut(s) 304
DpnI GATC 5 cut(s) 50, 262, 320, 461, 465
DpnII GATC 5 cut(s) 48, 260, 318, 459, 463
EaeI YGGCCR 1 cut(s) 471
EagI CGGCCG 1 cut(s) 471
EciI GGCGGA 1 cut(s) 205
EclXI CGGCCG 1 cut(s) 471
Eco52I CGGCCG 1 cut(s) 471
Eco72I CACGTG 1 cut(s) 309
Eco88I CYCGRG 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 312
EgeI GGCGCC 1 cut(s) 304
EheI GGCGCC 1 cut(s) 304
Esp3I CGTCTC 1 cut(s) 217
FaeI CATG 1 cut(s) 394
FaiI YATR 3 cut(s) 59, 110, 392
FatI CATG 1 cut(s) 390
FauI CCCGC 3 cut(s) 24, 179, 260
Fnu4HI GCNGC 1 cut(s) 245
FokI GGATG 1 cut(s) 361
Fsp4HI GCNGC 1 cut(s) 245
FspAI RTGCGCAY 1 cut(s) 226
FspBI CTAG 1 cut(s) 529
FspI TGCGCA 1 cut(s) 226
GlaI GCGC 4 cut(s) 175, 226, 304, 439
GluI GCNGC 1 cut(s) 245
HaeII RGCGCY 1 cut(s) 306
HaeIII GGCC 3 cut(s) 202, 244, 473
HapII CCGG 3 cut(s) 199, 300, 359
HgaI GACGC 2 cut(s) 20, 199
HhaI GCGC 4 cut(s) 176, 227, 305, 440
Hin1I GRCGYC 2 cut(s) 12, 303
Hin1II CATG 1 cut(s) 394
Hin6I GCGC 4 cut(s) 174, 225, 303, 438
HinP1I GCGC 4 cut(s) 174, 225, 303, 438
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 3 cut(s) 22, 54, 292
HpaII CCGG 3 cut(s) 199, 300, 359
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 4 cut(s) 27, 53, 424, 468
Hpy188III TCNNGA 2 cut(s) 289, 391
Hpy8I GTNNAC 1 cut(s) 400
Hpy99I CGWCG 5 cut(s) 14, 158, 235, 284, 524
HpyCH4III ACNGT 2 cut(s) 235, 276
HpyCH4IV ACGT 3 cut(s) 282, 308, 363
HpyCH4V TGCA 1 cut(s) 355
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 224, 309
HpyF3I CTNAG 1 cut(s) 20
HpySE526I ACGT 3 cut(s) 282, 308, 363
Hsp92I GRCGYC 2 cut(s) 12, 303
Hsp92II CATG 1 cut(s) 394
HspAI GCGC 4 cut(s) 174, 225, 303, 438
KasI GGCGCC 1 cut(s) 302
KroI GCCGGC 1 cut(s) 299
KroNI GCCGGC 1 cut(s) 301
KspI CCGCGG 1 cut(s) 381
Kzo9I GATC 5 cut(s) 48, 260, 318, 459, 463
LmnI GCTCC 2 cut(s) 181, 272
LpnPI CCDG 5 cut(s) 212, 299, 313, 326, 372
LweI GCATC 1 cut(s) 236
MaeI CTAG 1 cut(s) 529
MaeII ACGT 3 cut(s) 282, 308, 363
MalI GATC 5 cut(s) 50, 262, 320, 461, 465
MboI GATC 5 cut(s) 48, 260, 318, 459, 463
MflI RGATCY 1 cut(s) 48
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 1 cut(s) 69
Mly113I GGCGCC 1 cut(s) 303
MlyI GAGTC 2 cut(s) 31, 48
MmeI TCCRAC 2 cut(s) 76, 447
MreI CGCCGGCG 1 cut(s) 299
MroNI GCCGGC 1 cut(s) 299
MspA1I CMGCKG 2 cut(s) 19, 380
MspI CCGG 3 cut(s) 199, 300, 359
MspR9I CCNGG 2 cut(s) 314, 359
MvaI CCWGG 1 cut(s) 314
MvnI CGCG 5 cut(s) 174, 210, 247, 380, 438
MwoI GCNNNNNNNGC 3 cut(s) 165, 224, 309
NaeI GCCGGC 1 cut(s) 301
NarI GGCGCC 1 cut(s) 303
NciI CCSGG 1 cut(s) 359
NdeII GATC 5 cut(s) 48, 260, 318, 459, 463
NgoMIV GCCGGC 1 cut(s) 299
NlaIII CATG 1 cut(s) 394
NlaIV GGNNCC 2 cut(s) 203, 304
NmeAIII GCCGAG 1 cut(s) 499
NsbI TGCGCA 1 cut(s) 226
NspV TTCGAA 1 cut(s) 74
PagI TCATGA 1 cut(s) 390
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PdiI GCCGGC 1 cut(s) 301
PfeI GAWTC 1 cut(s) 292
PkrI GCNGC 1 cut(s) 246
Ple19I CGATCG 1 cut(s) 466
PleI GAGTC 2 cut(s) 30, 48
PluTI GGCGCC 1 cut(s) 306
PmaCI CACGTG 1 cut(s) 309
PmlI CACGTG 1 cut(s) 309
PpsI GAGTC 2 cut(s) 30, 48
Ppu21I YACGTR 1 cut(s) 309
Psp6I CCWGG 1 cut(s) 312
PspCI CACGTG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 2 cut(s) 203, 304
PspPI GGNCC 1 cut(s) 201
PsuI RGATCY 1 cut(s) 48
PvuI CGATCG 1 cut(s) 466
RsaI GTAC 2 cut(s) 278, 332
RsaNI GTAC 2 cut(s) 277, 331
SacII CCGCGG 1 cut(s) 381
SatI GCNGC 1 cut(s) 245
Sau3AI GATC 5 cut(s) 48, 260, 318, 459, 463
Sau96I GGNCC 1 cut(s) 201
SchI GAGTC 2 cut(s) 31, 48
ScrFI CCNGG 2 cut(s) 314, 359
SduI GDGCHC 1 cut(s) 288
SetI ASST 6 cut(s) 197, 269, 285, 311, 366, 409
SfaNI GCATC 1 cut(s) 236
SfoI GGCGCC 1 cut(s) 304
Sfr303I CCGCGG 1 cut(s) 381
SfuI TTCGAA 1 cut(s) 74
SgrAI CRCCGGYG 1 cut(s) 299
SgrBI CCGCGG 1 cut(s) 381
Sse9I AATT 1 cut(s) 69
SspDI GGCGCC 1 cut(s) 302
SspMI CTAG 1 cut(s) 529
StyD4I CCNGG 2 cut(s) 312, 357
TaaI ACNGT 2 cut(s) 235, 276
TaiI ACGT 3 cut(s) 285, 311, 366
TaqI TCGA 5 cut(s) 74, 230, 237, 462, 522
TasI AATT 1 cut(s) 69
TauI GCSGC 1 cut(s) 247
TfiI GAWTC 1 cut(s) 292
TscAI CASTG 1 cut(s) 279
TspDTI ATGAA 3 cut(s) 379, 407, 481
TspGWI ACGGA 2 cut(s) 142, 178
TspRI CASTG 1 cut(s) 279
XapI RAATTY 1 cut(s) 69
XspI CTAG 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.