Rw6G037470

Chaperone protein dnaJ 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0017105
Physical Location & Seq
Forward (+)
61775997 .. 61777020
1024 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G037470.1

Sequence Viewer

Length: 462 bp
ATGATGTCCATTTCATCTTCGTCATTCACCGCCTCCTCATCTCTCTTTTCCGGCCGGACCCTCGTCCTTGACGCTCCGAACTCGCCGTCGTCCATCAAGTACCGTCCGATGAGCATCTCGGCCTCATGCGCATCAACGGTCGACATGCCCCGCATCCACATTGGGACAAATCCGTCCCTCTATGAAGTCCTCGGCATTCACACGGGCGCCACGTGTCAGGAAATCAAGACCGCTTACCGCAGATTGGCCAGAGTCGTGCACCCTGACGCCACCGCAGCTAACGGTCAAAGTTCCTCCGACGAGTTCATGAAGGTCCACGAGGCTTATGCCACTCTCTCCGACGCCCAAAAGCGCGCCGACTACGACCGCAGGATGCTTATCCGGCGGCCGCTGCCGATGAGCTCTTCCTTCTCAATGTCGTCGTCGACGGGAAGGAGATGGGAAACCGATCAGTGCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

153

Amino Acids

16.85

Weight (kDa)

9.18

Isoelectric Point (pI)

72.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 60 - 122 1.1e-20 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g38340
prunus_persica Prupe.1G364400_v2.0.a1
pyrus_communis pycom08g01060 pycom15g01000
rosa_chinensis RchiOBHm_Chr6g0303011
rosa_laevigata RLG00000011118
rosa_multiflora Rmu_ssc0000460.1_g000030
rosa_roxburghii Rroxscaffold_7G00165110
rosa_rugosa Rorug06G0318400
rosa_samantha Rh6AG432200 Rh6BG470300 Rh6DG431400
rosa_wichuraiana Rw6G037470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 172
Acc16I TGCGCA 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 206
AccI GTMKAC 2 cut(s) 141, 425
AccII CGCG 1 cut(s) 354
AciI CCGC 8 cut(s) 30, 151, 231, 238, 273, 367, 385, 389
AcoI YGGCCR 3 cut(s) 52, 246, 386
AcvI CACGTG 1 cut(s) 213
AcyI GRCGYC 3 cut(s) 207, 267, 342
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 244
AflIII ACRYGT 1 cut(s) 212
AluBI AGCT 2 cut(s) 278, 402
AluI AGCT 2 cut(s) 278, 402
Alw21I GWGCWC 2 cut(s) 261, 404
Alw44I GTGCAC 1 cut(s) 257
AoxI GGCC 4 cut(s) 52, 120, 246, 386
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 2 cut(s) 275, 391
ArsI GACNNNNNNTTYG 2 cut(s) 71, 103
AspLEI GCGC 4 cut(s) 131, 209, 354, 356
AspS9I GGNCC 2 cut(s) 57, 313
AsuHPI GGTGA 1 cut(s) 19
AvaII GGWCC 2 cut(s) 57, 313
BaeGI GKGCMC 1 cut(s) 261
BalI TGGCCA 1 cut(s) 248
BanI GGYRCC 1 cut(s) 206
BanII GRGCYC 1 cut(s) 404
BauI CACGAG 1 cut(s) 317
BbrPI CACGTG 1 cut(s) 213
Bbv12I GWGCWC 2 cut(s) 261, 404
BbvI GCAGC 2 cut(s) 287, 378
BccI CCATC 2 cut(s) 101, 432
BceAI ACGGC 1 cut(s) 70
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BfoI RGCGCY 1 cut(s) 210
BisI GCNGC 4 cut(s) 276, 386, 389, 392
BlsI GCNGC 4 cut(s) 277, 387, 390, 393
Bme18I GGWCC 2 cut(s) 57, 313
BmgT120I GGNCC 2 cut(s) 57, 313
BmiI GGNNCC 2 cut(s) 59, 208
BmsI GCATC 4 cut(s) 123, 140, 162, 363
BoxI GACNNNNGTC 1 cut(s) 62
BsaAI YACGTR 1 cut(s) 213
BsaBI GATNNNNATC 2 cut(s) 113, 377
BsaHI GRCGYC 3 cut(s) 207, 267, 342
BsaJI CCNNGG 1 cut(s) 190
Bsc4I CCNNNNNNNGG 1 cut(s) 244
Bse8I GATNNNNATC 2 cut(s) 113, 377
BseDI CCNNGG 1 cut(s) 190
BseGI GGATG 2 cut(s) 153, 378
BseJI GATNNNNATC 2 cut(s) 113, 377
BseLI CCNNNNNNNGG 1 cut(s) 244
BsePI GCGCGC 1 cut(s) 352
BseRI GAGGAG 1 cut(s) 25
BseSI GKGCMC 1 cut(s) 261
BseX3I CGGCCG 2 cut(s) 52, 386
BseXI GCAGC 2 cut(s) 287, 378
Bsh1236I CGCG 1 cut(s) 354
Bsh1285I CGRYCG 4 cut(s) 55, 141, 367, 389
BshFI GGCC 4 cut(s) 54, 122, 248, 388
BshNI GGYRCC 1 cut(s) 206
BsiEI CGRYCG 4 cut(s) 55, 141, 367, 389
BsiHKAI GWGCWC 2 cut(s) 261, 404
BsiSI CCGG 3 cut(s) 51, 55, 382
BslFI GGGAC 2 cut(s) 160, 178
BslI CCNNNNNNNGG 1 cut(s) 244
BsmFI GGGAC 2 cut(s) 160, 178
BsmI GAATGC 1 cut(s) 195
BsnI GGCC 4 cut(s) 54, 122, 248, 388
Bsp1286I GDGCHC 2 cut(s) 261, 404
Bsp143I GATC 1 cut(s) 448
BspACI CCGC 8 cut(s) 30, 151, 231, 238, 273, 367, 385, 389
BspANI GGCC 4 cut(s) 54, 122, 248, 388
BspFNI CGCG 1 cut(s) 354
BspHI TCATGA 1 cut(s) 306
BspLI GGNNCC 2 cut(s) 59, 208
BspQI GCTCTTC 1 cut(s) 409
BspT107I GGYRCC 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 190
BssHII GCGCGC 1 cut(s) 352
BssMI GATC 1 cut(s) 448
BssNI GRCGYC 3 cut(s) 207, 267, 342
BssSI CACGAG 1 cut(s) 317
Bst2BI CACGAG 1 cut(s) 317
Bst4CI ACNGT 3 cut(s) 104, 139, 284
Bst6I CTCTTC 1 cut(s) 409
BstACI GRCGYC 3 cut(s) 207, 267, 342
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 1 cut(s) 354
BstF5I GGATG 2 cut(s) 153, 378
BstFNI CGCG 1 cut(s) 354
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 4 cut(s) 131, 209, 354, 356
BstKTI GATC 1 cut(s) 451
BstMBI GATC 1 cut(s) 448
BstMCI CGRYCG 4 cut(s) 55, 141, 367, 389
BstMWI GCNNNNNNNGC 4 cut(s) 128, 275, 382, 391
BstNSI RCATGY 1 cut(s) 148
BstPAI GACNNNNGTC 1 cut(s) 62
BstSLI GKGCMC 1 cut(s) 261
BstUI CGCG 1 cut(s) 354
BstV1I GCAGC 2 cut(s) 287, 378
BstZI CGGCCG 2 cut(s) 52, 386
BsuRI GGCC 4 cut(s) 54, 122, 248, 388
BtsCI GGATG 2 cut(s) 153, 378
BtsIMutI CAGTG 1 cut(s) 458
Cac8I GCNNGC 1 cut(s) 354
CciI TCATGA 1 cut(s) 306
CciNI GCGGCCGC 1 cut(s) 386
CfoI GCGC 4 cut(s) 131, 209, 354, 356
Cfr13I GGNCC 2 cut(s) 57, 313
CseI GACGC 3 cut(s) 80, 275, 350
Csp6I GTAC 1 cut(s) 100
CviAII CATG 3 cut(s) 126, 145, 307
CviJI RGCY 7 cut(s) 54, 122, 248, 278, 323, 388, 402
CviKI_1 RGCY 7 cut(s) 54, 122, 248, 278, 323, 388, 402
CviQI GTAC 1 cut(s) 100
DinI GGCGCC 1 cut(s) 208
DpnI GATC 1 cut(s) 450
DpnII GATC 1 cut(s) 448
DrdI GACNNNNNNGTC 1 cut(s) 172
DseDI GACNNNNNNGTC 1 cut(s) 172
EaeI YGGCCR 3 cut(s) 52, 246, 386
EagI CGGCCG 2 cut(s) 52, 386
Eam1104I CTCTTC 1 cut(s) 409
EarI CTCTTC 1 cut(s) 409
Ecl136II GAGCTC 1 cut(s) 402
EclXI CGGCCG 2 cut(s) 52, 386
Eco24I GRGCYC 1 cut(s) 404
Eco47I GGWCC 2 cut(s) 57, 313
Eco52I CGGCCG 2 cut(s) 52, 386
Eco53kI GAGCTC 1 cut(s) 402
Eco72I CACGTG 1 cut(s) 213
EcoICRI GAGCTC 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 404
EgeI GGCGCC 1 cut(s) 208
EheI GGCGCC 1 cut(s) 208
FaeI CATG 3 cut(s) 129, 148, 310
FaiI YATR 5 cut(s) 127, 146, 183, 308, 327
FaqI GGGAC 2 cut(s) 160, 178
FatI CATG 3 cut(s) 125, 144, 306
FauI CCCGC 1 cut(s) 158
FblI GTMKAC 2 cut(s) 141, 425
Fnu4HI GCNGC 4 cut(s) 276, 386, 389, 392
FokI GGATG 2 cut(s) 140, 385
FriOI GRGCYC 1 cut(s) 404
Fsp4HI GCNGC 4 cut(s) 276, 386, 389, 392
FspAI RTGCGCAY 1 cut(s) 130
FspI TGCGCA 1 cut(s) 130
GlaI GCGC 4 cut(s) 130, 208, 353, 355
GluI GCNGC 4 cut(s) 276, 386, 389, 392
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 4 cut(s) 54, 122, 248, 388
HapII CCGG 3 cut(s) 51, 55, 382
HgaI GACGC 3 cut(s) 80, 275, 350
HhaI GCGC 4 cut(s) 131, 209, 354, 356
Hin1I GRCGYC 3 cut(s) 207, 267, 342
Hin1II CATG 3 cut(s) 129, 148, 310
Hin6I GCGC 4 cut(s) 129, 207, 352, 354
HinP1I GCGC 4 cut(s) 129, 207, 352, 354
HincII GTYRAC 2 cut(s) 142, 426
HindII GTYRAC 2 cut(s) 142, 426
HinfI GANTC 1 cut(s) 252
HpaII CCGG 3 cut(s) 51, 55, 382
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 4 cut(s) 142, 259, 316, 426
Hpy188I TCNGA 4 cut(s) 78, 108, 298, 340
Hpy188III TCNNGA 3 cut(s) 218, 226, 307
Hpy8I GTNNAC 4 cut(s) 142, 259, 316, 426
Hpy99I CGWCG 6 cut(s) 91, 302, 344, 424, 427, 430
HpyAV CCTTC 3 cut(s) 304, 418, 426
HpyCH4III ACNGT 3 cut(s) 104, 139, 284
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 1 cut(s) 259
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 275, 382, 391
HpySE526I ACGT 1 cut(s) 212
Hsp92I GRCGYC 3 cut(s) 207, 267, 342
Hsp92II CATG 3 cut(s) 129, 148, 310
HspAI GCGC 4 cut(s) 129, 207, 352, 354
KasI GGCGCC 1 cut(s) 206
Kzo9I GATC 1 cut(s) 448
LguI GCTCTTC 1 cut(s) 409
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 8 cut(s) 64, 68, 203, 262, 276, 355, 395, 442
Lsp1109I GCAGC 2 cut(s) 287, 378
LweI GCATC 4 cut(s) 123, 140, 162, 363
MaeII ACGT 1 cut(s) 212
MalI GATC 1 cut(s) 450
MboI GATC 1 cut(s) 448
MboII GAAGA 2 cut(s) 9, 396
MhlI GDGCHC 2 cut(s) 261, 404
MlsI TGGCCA 1 cut(s) 248
MluNI TGGCCA 1 cut(s) 248
Mly113I GGCGCC 1 cut(s) 207
MlyI GAGTC 1 cut(s) 261
MmeI TCCRAC 2 cut(s) 321, 363
MnlI CCTC 8 cut(s) 43, 46, 71, 133, 188, 200, 304, 313
Mox20I TGGCCA 1 cut(s) 248
MscI TGGCCA 1 cut(s) 248
Msp20I TGGCCA 1 cut(s) 248
MspA1I CMGCKG 1 cut(s) 391
MspI CCGG 3 cut(s) 51, 55, 382
Mva1269I GAATGC 1 cut(s) 195
MvnI CGCG 1 cut(s) 354
MwoI GCNNNNNNNGC 4 cut(s) 128, 275, 382, 391
NarI GGCGCC 1 cut(s) 207
NdeII GATC 1 cut(s) 448
NlaIII CATG 3 cut(s) 129, 148, 310
NlaIV GGNNCC 2 cut(s) 59, 208
NmeAIII GCCGAG 2 cut(s) 98, 171
NotI GCGGCCGC 1 cut(s) 386
NsbI TGCGCA 1 cut(s) 130
NspI RCATGY 1 cut(s) 148
PagI TCATGA 1 cut(s) 306
PauI GCGCGC 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 409
PcsI WCGNNNNNNNCGW 1 cut(s) 209
PctI GAATGC 1 cut(s) 195
PkrI GCNGC 4 cut(s) 277, 387, 390, 393
PleI GAGTC 1 cut(s) 260
PluTI GGCGCC 1 cut(s) 210
PmaCI CACGTG 1 cut(s) 213
PmlI CACGTG 1 cut(s) 213
PpsI GAGTC 1 cut(s) 260
Ppu21I YACGTR 1 cut(s) 213
PshAI GACNNNNGTC 1 cut(s) 62
Psp124BI GAGCTC 1 cut(s) 404
PspCI CACGTG 1 cut(s) 213
PspN4I GGNNCC 2 cut(s) 59, 208
PspPI GGNCC 2 cut(s) 57, 313
PteI GCGCGC 1 cut(s) 352
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SacI GAGCTC 1 cut(s) 404
SalI GTCGAC 2 cut(s) 140, 424
SapI GCTCTTC 1 cut(s) 409
SatI GCNGC 4 cut(s) 276, 386, 389, 392
Sau3AI GATC 1 cut(s) 448
Sau96I GGNCC 2 cut(s) 57, 313
SchI GAGTC 1 cut(s) 261
SduI GDGCHC 2 cut(s) 261, 404
SetI ASST 4 cut(s) 215, 280, 315, 404
SfaNI GCATC 4 cut(s) 123, 140, 162, 363
SfoI GGCGCC 1 cut(s) 208
SgrDI CGTCGACG 1 cut(s) 424
SinI GGWCC 2 cut(s) 57, 313
SsiI CCGC 8 cut(s) 30, 151, 231, 238, 273, 367, 385, 389
SspDI GGCGCC 1 cut(s) 206
SstI GAGCTC 1 cut(s) 404
TaaI ACNGT 3 cut(s) 104, 139, 284
TaiI ACGT 1 cut(s) 215
TaqI TCGA 2 cut(s) 141, 425
TauI GCSGC 2 cut(s) 388, 391
TscAI CASTG 1 cut(s) 458
TseI GCWGC 2 cut(s) 275, 391
TspDTI ATGAA 3 cut(s) 198, 295, 323
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 1 cut(s) 458
VneI GTGCAC 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 57, 313
XceI RCATGY 1 cut(s) 148
XmiI GTMKAC 2 cut(s) 141, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.