pycom15g01000

Chaperone protein dnaJ 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0017105
Physical Location & Seq
Reverse (-)
533020 .. 533466
447 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g01000.1

Sequence Viewer

Length: 447 bp
ATGGCTTCCATATCCTCCTCATCCTTCACTTGCTCCTCTTCCCCTTTTTTCTCCGTCGTCAACGGCGGCCCACGCGCCCCTCCCTCCTCCGTCAGCTACCGCGTCTCCGCCTCGTGCTCATCGACGGTCGACAAGCCGCGCTCCCGCATTGGATCAAGCTCTTCACTCTACGAGGTGCTCGGGATTCACGCCGGCGCCACGTGTCATGAGATAAAGTCGGCGTACCGTAAATTAGCGAGAGTTGTGCACCCGGACGTTGCGAAATCTTCGGCCGACGAGTTCATGAAAGTCCACGAAGCTTACTCCACTCTCTCCGACGCCCAAAAACGCGCCGATTACGACCGCAAGATGTTGATCGGACGGCCGCGTACGGGCGTGGCTTCATCATACGCTTACGCTACCACAATGTCGACTGGCCGGAGATGGGAAACCGACCAGTGCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

149

Amino Acids

16.06

Weight (kDa)

9.44

Isoelectric Point (pI)

54.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 56 - 114 1.9e-20 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g38340
prunus_persica Prupe.1G364400_v2.0.a1
pyrus_communis pycom08g01060 pycom15g01000
rosa_chinensis RchiOBHm_Chr6g0303011
rosa_laevigata RLG00000011118
rosa_multiflora Rmu_ssc0000460.1_g000030
rosa_roxburghii Rroxscaffold_7G00165110
rosa_rugosa Rorug06G0318400
rosa_samantha Rh6AG432200 Rh6BG470300 Rh6DG431400
rosa_wichuraiana Rw6G037470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 194
AccI GTMKAC 2 cut(s) 129, 410
AccII CGCG 5 cut(s) 75, 102, 139, 330, 367
AciI CCGC 7 cut(s) 66, 100, 108, 137, 145, 343, 365
AclWI GGATC 1 cut(s) 160
AcoI YGGCCR 3 cut(s) 270, 362, 415
AcvI CACGTG 1 cut(s) 201
AcyI GRCGYC 2 cut(s) 195, 318
AfaI GTAC 2 cut(s) 224, 370
AfiI CCNNNNNNNGG 2 cut(s) 371, 424
AflIII ACRYGT 1 cut(s) 200
AluBI AGCT 3 cut(s) 96, 159, 299
AluI AGCT 3 cut(s) 96, 159, 299
Alw21I GWGCWC 3 cut(s) 119, 180, 249
Alw26I GTCTC 1 cut(s) 109
Alw44I GTGCAC 1 cut(s) 245
AlwI GGATC 1 cut(s) 160
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 4 cut(s) 67, 270, 362, 415
ApaLI GTGCAC 1 cut(s) 245
AspLEI GCGC 4 cut(s) 77, 141, 197, 332
AspS9I GGNCC 1 cut(s) 68
AsuC2I CCSGG 1 cut(s) 251
AvaI CYCGRG 1 cut(s) 179
BaeGI GKGCMC 1 cut(s) 249
BanI GGYRCC 1 cut(s) 194
BauI CACGAG 1 cut(s) 112
BbrPI CACGTG 1 cut(s) 201
Bbv12I GWGCWC 3 cut(s) 119, 180, 249
BccI CCATC 1 cut(s) 417
BceAI ACGGC 2 cut(s) 79, 377
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BcnI CCSGG 1 cut(s) 251
BcoDI GTCTC 1 cut(s) 109
BfoI RGCGCY 1 cut(s) 198
BisI GCNGC 3 cut(s) 67, 137, 365
BlsI GCNGC 3 cut(s) 68, 138, 366
Bme1390I CCNGG 1 cut(s) 251
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 1 cut(s) 196
BmrFI CCNGG 1 cut(s) 251
BpuMI CCSGG 1 cut(s) 251
BsaAI YACGTR 1 cut(s) 201
BsaBI GATNNNNATC 1 cut(s) 353
BsaHI GRCGYC 2 cut(s) 195, 318
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 2 cut(s) 371, 424
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 2 cut(s) 418, 436
Bse8I GATNNNNATC 1 cut(s) 353
BseGI GGATG 1 cut(s) 20
BseJI GATNNNNATC 1 cut(s) 353
BseLI CCNNNNNNNGG 2 cut(s) 371, 424
BseNI ACTGG 2 cut(s) 418, 436
BseRI GAGGAG 3 cut(s) 7, 25, 76
BseSI GKGCMC 1 cut(s) 249
BseX3I CGGCCG 2 cut(s) 270, 362
Bsh1236I CGCG 5 cut(s) 75, 102, 139, 330, 367
Bsh1285I CGRYCG 4 cut(s) 129, 273, 343, 365
BshFI GGCC 4 cut(s) 69, 272, 364, 417
BshNI GGYRCC 1 cut(s) 194
BsiEI CGRYCG 4 cut(s) 129, 273, 343, 365
BsiHKAI GWGCWC 3 cut(s) 119, 180, 249
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 3 cut(s) 192, 251, 418
BsiWI CGTACG 1 cut(s) 368
BslI CCNNNNNNNGG 2 cut(s) 371, 424
BsmAI GTCTC 1 cut(s) 109
BsmBI CGTCTC 1 cut(s) 109
BsnI GGCC 4 cut(s) 69, 272, 364, 417
BsoBI CYCGRG 1 cut(s) 179
Bsp1286I GDGCHC 3 cut(s) 119, 180, 249
Bsp143I GATC 2 cut(s) 152, 354
BspACI CCGC 7 cut(s) 66, 100, 108, 137, 145, 343, 365
BspANI GGCC 4 cut(s) 69, 272, 364, 417
BspFNI CGCG 5 cut(s) 75, 102, 139, 330, 367
BspHI TCATGA 2 cut(s) 205, 282
BspLI GGNNCC 1 cut(s) 196
BspPI GGATC 1 cut(s) 160
BspQI GCTCTTC 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 194
BsrFI RCCGGY 1 cut(s) 191
BsrI ACTGG 2 cut(s) 418, 436
BssAI RCCGGY 1 cut(s) 191
BssMI GATC 2 cut(s) 152, 354
BssNI GRCGYC 2 cut(s) 195, 318
BssSI CACGAG 1 cut(s) 112
Bst2BI CACGAG 1 cut(s) 112
Bst4CI ACNGT 2 cut(s) 127, 227
Bst6I CTCTTC 2 cut(s) 43, 166
BstACI GRCGYC 2 cut(s) 195, 318
BstBAI YACGTR 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 193
BstF5I GGATG 1 cut(s) 20
BstFNI CGCG 5 cut(s) 75, 102, 139, 330, 367
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 4 cut(s) 77, 141, 197, 332
BstKTI GATC 2 cut(s) 155, 357
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 2 cut(s) 152, 354
BstMCI CGRYCG 4 cut(s) 129, 273, 343, 365
BstMWI GCNNNNNNNGC 1 cut(s) 72
BstSCI CCNGG 1 cut(s) 249
BstSLI GKGCMC 1 cut(s) 249
BstUI CGCG 5 cut(s) 75, 102, 139, 330, 367
BstZI CGGCCG 2 cut(s) 270, 362
BsuRI GGCC 4 cut(s) 69, 272, 364, 417
BtsCI GGATG 1 cut(s) 20
BtsIMutI CAGTG 1 cut(s) 443
Cac8I GCNNGC 1 cut(s) 193
CciI TCATGA 2 cut(s) 205, 282
CfoI GCGC 4 cut(s) 77, 141, 197, 332
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 1 cut(s) 68
CseI GACGC 2 cut(s) 91, 326
Csp6I GTAC 2 cut(s) 223, 369
CviAII CATG 2 cut(s) 206, 283
CviQI GTAC 2 cut(s) 223, 369
DinI GGCGCC 1 cut(s) 196
DpnI GATC 2 cut(s) 154, 356
DpnII GATC 2 cut(s) 152, 354
EaeI YGGCCR 3 cut(s) 270, 362, 415
EagI CGGCCG 2 cut(s) 270, 362
Eam1104I CTCTTC 2 cut(s) 43, 166
EarI CTCTTC 2 cut(s) 43, 166
EciI GGCGGA 1 cut(s) 97
EclXI CGGCCG 2 cut(s) 270, 362
Eco52I CGGCCG 2 cut(s) 270, 362
Eco72I CACGTG 1 cut(s) 201
Eco88I CYCGRG 1 cut(s) 179
EgeI GGCGCC 1 cut(s) 196
EheI GGCGCC 1 cut(s) 196
Esp3I CGTCTC 1 cut(s) 109
FaeI CATG 2 cut(s) 209, 286
FaiI YATR 4 cut(s) 11, 207, 284, 388
FatI CATG 2 cut(s) 205, 282
FauI CCCGC 1 cut(s) 152
FblI GTMKAC 2 cut(s) 129, 410
Fnu4HI GCNGC 3 cut(s) 67, 137, 365
FokI GGATG 1 cut(s) 7
Fsp4HI GCNGC 3 cut(s) 67, 137, 365
GlaI GCGC 4 cut(s) 76, 140, 196, 331
GluI GCNGC 3 cut(s) 67, 137, 365
HaeII RGCGCY 1 cut(s) 198
HaeIII GGCC 4 cut(s) 69, 272, 364, 417
HapII CCGG 3 cut(s) 192, 251, 418
HgaI GACGC 2 cut(s) 91, 326
HhaI GCGC 4 cut(s) 77, 141, 197, 332
Hin1I GRCGYC 2 cut(s) 195, 318
Hin1II CATG 2 cut(s) 209, 286
Hin6I GCGC 4 cut(s) 75, 139, 195, 330
HinP1I GCGC 4 cut(s) 75, 139, 195, 330
HincII GTYRAC 3 cut(s) 61, 130, 411
HindII GTYRAC 3 cut(s) 61, 130, 411
HindIII AAGCTT 1 cut(s) 297
HinfI GANTC 1 cut(s) 184
HpaII CCGG 3 cut(s) 192, 251, 418
Hpy166II GTNNAC 5 cut(s) 61, 130, 247, 292, 411
Hpy188I TCNGA 2 cut(s) 316, 359
Hpy188III TCNNGA 3 cut(s) 181, 206, 283
Hpy8I GTNNAC 5 cut(s) 61, 130, 247, 292, 411
Hpy99I CGWCG 4 cut(s) 59, 127, 278, 320
HpyAV CCTTC 1 cut(s) 34
HpyCH4III ACNGT 2 cut(s) 127, 227
HpyCH4IV ACGT 2 cut(s) 200, 255
HpyCH4V TGCA 1 cut(s) 247
HpyF10VI GCNNNNNNNGC 1 cut(s) 72
HpySE526I ACGT 2 cut(s) 200, 255
Hsp92I GRCGYC 2 cut(s) 195, 318
Hsp92II CATG 2 cut(s) 209, 286
HspAI GCGC 4 cut(s) 75, 139, 195, 330
KasI GGCGCC 1 cut(s) 194
KroI GCCGGC 1 cut(s) 191
KroNI GCCGGC 1 cut(s) 193
Kzo9I GATC 2 cut(s) 152, 354
LguI GCTCTTC 1 cut(s) 166
LmnI GCTCC 2 cut(s) 38, 146
LpnPI CCDG 5 cut(s) 205, 264, 399, 427, 431
MaeII ACGT 2 cut(s) 200, 255
MalI GATC 2 cut(s) 154, 356
MboI GATC 2 cut(s) 152, 354
MboII GAAGA 3 cut(s) 30, 153, 258
MhlI GDGCHC 3 cut(s) 119, 180, 249
MluCI AATT 1 cut(s) 230
Mly113I GGCGCC 1 cut(s) 195
MmeI TCCRAC 1 cut(s) 339
MnlI CCTC 8 cut(s) 25, 28, 46, 90, 94, 97, 121, 166
MreI CGCCGGCG 1 cut(s) 191
MroNI GCCGGC 1 cut(s) 191
MspI CCGG 3 cut(s) 192, 251, 418
MspR9I CCNGG 1 cut(s) 251
MvnI CGCG 5 cut(s) 75, 102, 139, 330, 367
MwoI GCNNNNNNNGC 1 cut(s) 72
NaeI GCCGGC 1 cut(s) 193
NarI GGCGCC 1 cut(s) 195
NciI CCSGG 1 cut(s) 251
NdeII GATC 2 cut(s) 152, 354
NgoMIV GCCGGC 1 cut(s) 191
NlaIII CATG 2 cut(s) 209, 286
NlaIV GGNNCC 1 cut(s) 196
PagI TCATGA 2 cut(s) 205, 282
PciSI GCTCTTC 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PdiI GCCGGC 1 cut(s) 193
PfeI GAWTC 1 cut(s) 184
Pfl23II CGTACG 1 cut(s) 368
PkrI GCNGC 3 cut(s) 68, 138, 366
PluTI GGCGCC 1 cut(s) 198
PmaCI CACGTG 1 cut(s) 201
PmlI CACGTG 1 cut(s) 201
Ppu21I YACGTR 1 cut(s) 201
PspCI CACGTG 1 cut(s) 201
PspLI CGTACG 1 cut(s) 368
PspN4I GGNNCC 1 cut(s) 196
PspPI GGNCC 1 cut(s) 68
RsaI GTAC 2 cut(s) 224, 370
RsaNI GTAC 2 cut(s) 223, 369
SalI GTCGAC 2 cut(s) 128, 409
SapI GCTCTTC 1 cut(s) 166
SatI GCNGC 3 cut(s) 67, 137, 365
Sau3AI GATC 2 cut(s) 152, 354
Sau96I GGNCC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 251
SduI GDGCHC 3 cut(s) 119, 180, 249
SetI ASST 6 cut(s) 98, 161, 177, 203, 258, 301
SfoI GGCGCC 1 cut(s) 196
SgrAI CRCCGGYG 1 cut(s) 191
Sse9I AATT 1 cut(s) 230
SsiI CCGC 7 cut(s) 66, 100, 108, 137, 145, 343, 365
SspDI GGCGCC 1 cut(s) 194
StyD4I CCNGG 1 cut(s) 249
TaaI ACNGT 2 cut(s) 127, 227
TaiI ACGT 2 cut(s) 203, 258
TaqI TCGA 3 cut(s) 122, 129, 410
TasI AATT 1 cut(s) 230
TauI GCSGC 3 cut(s) 69, 139, 367
TfiI GAWTC 1 cut(s) 184
TscAI CASTG 1 cut(s) 443
TspDTI ATGAA 3 cut(s) 271, 299, 372
TspGWI ACGGA 2 cut(s) 43, 79
TspRI CASTG 1 cut(s) 443
VneI GTGCAC 1 cut(s) 245
XmiI GTMKAC 2 cut(s) 129, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.