pycom08g07530

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008520
Physical Location & Seq
Reverse (-)
6062699 .. 6064711
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 978 bp
ATGGGTGGTTGGGCAATTGCGGTTCACGGCGGCGCGGGTGTCGACCCAAACCTCCCCATGGAGCGTCAGGAGCAGGCCAAACAACTCCTCACCCGGTGCCTCAATCTCGGCATCTCTGCTCTCCGCTCCGATCTCGCCGCCATCGATGTTGTCGAGCTCGTTGTTCGGGAATTGGAAGCCGATCCCTTCTTCAACTCTGGCCGTGGATCCGCTCTCACCGCTAAAGGGACGGTTGAGATGGAAGCCAGCATCATGGATGGACCCAAGAGACGGTGCGGCGCCGTTTCGGGCTTAACCACGGTTAAGAATCCCGTTTCACTCGCCCGCCTCGTCATGGACAAATCCCCTCATTCCTACCTCGCCTTCTCCGGCGCCGAAGACTTCGCCCGACAACAGGGCGTTGAGGTCGTGGACAATGAGTATTTCATCACGGAGGATAATGTGGGGATGCTCAAATTGGCCAAGGAGGCCAACACAATCTTGTTTGATTATAGGATTCCATTAGGATTGGAGAGTTGCAGCGCAGGTGTAGAGAGTCCGTTGGTCATGAACGGGCTTCCCATTAGAGTTTACGCCCCTGAGACCGTTGGGTGTGTGGTGGTAGATAGCCAAGGGCGTTGCGCTGCAGCTACATCCACCGGTGGGCTCATGAACAAAATGACTGGTCGAATTGGTGACTCGCCACTCATTGGTGCCGGTACCTATGCATGTAACCTTTGCGGGGTCTCGTGCACTGGAGAGGGCGAGGCAATCATACGCGGGACACTCGCGCGTGAGGTGGCAGCAGTTATGGAGTACAAGGGCCTGGGGCTGCAAGATGCAGTGGATTTTGTGATCAAAGAGAGGCTTGATGATGGCAAGGCCGGACTCATAGCCGTGTCTAACAAGGGTGAAGTAGCTTATGGGTTTAATTGTATGGGAATGTTTAGGGGGTGTGCTACCGAGGATGGGTTCATGGAGGTTGAGATTTGGGAGTAG

Protein Analysis

326

Amino Acids

34.41

Weight (kDa)

4.78

Isoelectric Point (pI)

33.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 4 - 321 1.2e-100 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 515
Acc36I ACCTGC 1 cut(s) 515
Acc65I GGTACC 1 cut(s) 698
AccB1I GGYRCC 5 cut(s) 96, 278, 371, 692, 698
AccB7I CCANNNNNTGG 1 cut(s) 689
AccBSI CCGCTC 2 cut(s) 126, 212
AccI GTMKAC 1 cut(s) 42
AccII CGCG 4 cut(s) 35, 759, 770, 772
AclWI GGATC 3 cut(s) 176, 201, 214
AcoI YGGCCR 2 cut(s) 199, 459
AcyI GRCGYC 2 cut(s) 279, 372
AdeI CACNNNGTG 1 cut(s) 96
AfaI GTAC 2 cut(s) 700, 797
AfiI CCNNNNNNNGG 9 cut(s) 58, 225, 270, 334, 394, 642, 689, 721, 948
AgeI ACCGGT 1 cut(s) 638
AgsI TTSAA 1 cut(s) 193
AjnI CCWGG 1 cut(s) 804
AluBI AGCT 3 cut(s) 157, 629, 899
AluI AGCT 3 cut(s) 157, 629, 899
Alw21I GWGCWC 2 cut(s) 159, 734
Alw26I GTCTC 3 cut(s) 262, 575, 730
Alw44I GTGCAC 1 cut(s) 730
AlwI GGATC 3 cut(s) 176, 201, 214
AoxI GGCC 6 cut(s) 75, 199, 459, 468, 802, 861
ApaLI GTGCAC 1 cut(s) 730
ApeKI GCWGC 5 cut(s) 519, 623, 626, 782, 811
AsiGI ACCGGT 1 cut(s) 638
Asp718I GGTACC 1 cut(s) 698
AspLEI GCGC 6 cut(s) 35, 281, 374, 524, 623, 772
AspS9I GGNCC 2 cut(s) 260, 802
AsuC2I CCSGG 1 cut(s) 94
AsuHPI GGTGA 4 cut(s) 82, 208, 686, 902
AvaII GGWCC 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 734
BalI TGGCCA 1 cut(s) 461
BamHI GGATCC 1 cut(s) 206
BanI GGYRCC 5 cut(s) 96, 278, 371, 692, 698
BanII GRGCYC 2 cut(s) 159, 648
BauI CACGAG 1 cut(s) 727
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 2 cut(s) 159, 734
BbvI GCAGC 5 cut(s) 531, 610, 638, 794, 798
BccI CCATC 5 cut(s) 149, 232, 251, 848, 941
BceAI ACGGC 4 cut(s) 43, 186, 266, 860
BciT130I CCWGG 1 cut(s) 806
BclI TGATCA 1 cut(s) 834
BcnI CCSGG 1 cut(s) 94
BcoDI GTCTC 3 cut(s) 262, 575, 730
BfmI CTRYAG 1 cut(s) 624
BfoI RGCGCY 2 cut(s) 282, 375
BfuAI ACCTGC 1 cut(s) 515
BglI GCCNNNNNGGC 1 cut(s) 467
BisI GCNGC 8 cut(s) 31, 138, 277, 520, 624, 627, 783, 812
BlsI GCNGC 8 cut(s) 32, 139, 278, 521, 625, 628, 784, 813
Bme1390I CCNGG 2 cut(s) 94, 806
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 260, 802
BmiI GGNNCC 7 cut(s) 98, 208, 262, 280, 373, 694, 700
BmrFI CCNGG 2 cut(s) 94, 806
BmsI GCATC 4 cut(s) 120, 258, 438, 808
BpiI GAAGAC 1 cut(s) 384
BpmI CTGGAG 1 cut(s) 756
BpuMI CCSGG 1 cut(s) 94
Bsa29I ATCGAT 1 cut(s) 144
BsaHI GRCGYC 2 cut(s) 279, 372
BsaI GGTCTC 2 cut(s) 575, 730
BsaJI CCNNGG 7 cut(s) 57, 202, 297, 462, 610, 805, 942
BsaWI WCCGGW 1 cut(s) 638
Bsc4I CCNNNNNNNGG 9 cut(s) 58, 225, 270, 334, 394, 642, 689, 721, 948
Bse118I RCCGGY 2 cut(s) 638, 695
Bse1I ACTGG 2 cut(s) 667, 739
BseBI CCWGG 1 cut(s) 806
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 7 cut(s) 57, 202, 297, 462, 610, 805, 942
BseGI GGATG 4 cut(s) 262, 453, 632, 952
BseLI CCNNNNNNNGG 9 cut(s) 58, 225, 270, 334, 394, 642, 689, 721, 948
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 2 cut(s) 667, 739
BseRI GAGGAG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 734
BseXI GCAGC 5 cut(s) 531, 610, 638, 794, 798
Bsh1236I CGCG 4 cut(s) 35, 759, 770, 772
BshFI GGCC 6 cut(s) 77, 201, 461, 470, 804, 863
BshNI GGYRCC 5 cut(s) 96, 278, 371, 692, 698
BshTI ACCGGT 1 cut(s) 638
BshVI ATCGAT 1 cut(s) 144
BsiHKAI GWGCWC 2 cut(s) 159, 734
BsiSI CCGG 5 cut(s) 94, 369, 639, 696, 864
BslFI GGGAC 2 cut(s) 241, 775
BslI CCNNNNNNNGG 9 cut(s) 58, 225, 270, 334, 394, 642, 689, 721, 948
BsmAI GTCTC 3 cut(s) 262, 575, 730
BsmBI CGTCTC 1 cut(s) 262
BsmFI GGGAC 2 cut(s) 241, 775
BsnI GGCC 6 cut(s) 77, 201, 461, 470, 804, 863
Bso31I GGTCTC 2 cut(s) 575, 730
Bsp1286I GDGCHC 3 cut(s) 159, 648, 734
Bsp143I GATC 4 cut(s) 130, 181, 206, 834
Bsp19I CCATGG 1 cut(s) 57
BspANI GGCC 6 cut(s) 77, 201, 461, 470, 804, 863
BspCNI CTCAG 1 cut(s) 571
BspDI ATCGAT 1 cut(s) 144
BspFNI CGCG 4 cut(s) 35, 759, 770, 772
BspHI TCATGA 2 cut(s) 546, 648
BspLI GGNNCC 7 cut(s) 98, 208, 262, 280, 373, 694, 700
BspMAI CTGCAG 1 cut(s) 628
BspMI ACCTGC 1 cut(s) 515
BspPI GGATC 3 cut(s) 176, 201, 214
BspT107I GGYRCC 5 cut(s) 96, 278, 371, 692, 698
BspTNI GGTCTC 2 cut(s) 575, 730
BsrBI CCGCTC 2 cut(s) 126, 212
BsrFI RCCGGY 2 cut(s) 638, 695
BsrI ACTGG 2 cut(s) 667, 739
BssAI RCCGGY 2 cut(s) 638, 695
BssECI CCNNGG 7 cut(s) 57, 202, 297, 462, 610, 805, 942
BssMI GATC 4 cut(s) 130, 181, 206, 834
BssNI GRCGYC 2 cut(s) 279, 372
BssSI CACGAG 1 cut(s) 727
BssT1I CCWWGG 3 cut(s) 57, 462, 610
Bst2BI CACGAG 1 cut(s) 727
Bst2UI CCWGG 1 cut(s) 806
Bst4CI ACNGT 4 cut(s) 232, 273, 301, 586
BstACI GRCGYC 2 cut(s) 279, 372
BstC8I GCNNGC 3 cut(s) 75, 247, 325
BstDEI CTNAG 1 cut(s) 579
BstDSI CCRYGG 3 cut(s) 57, 202, 297
BstF5I GGATG 4 cut(s) 262, 453, 632, 952
BstFNI CGCG 4 cut(s) 35, 759, 770, 772
BstH2I RGCGCY 2 cut(s) 282, 375
BstHHI GCGC 6 cut(s) 35, 281, 374, 524, 623, 772
BstKTI GATC 4 cut(s) 133, 184, 209, 837
BstMAI GTCTC 3 cut(s) 262, 575, 730
BstMBI GATC 4 cut(s) 130, 181, 206, 834
BstMWI GCNNNNNNNGC 3 cut(s) 70, 218, 467
BstNI CCWGG 1 cut(s) 806
BstNSI RCATGY 1 cut(s) 711
BstSCI CCNGG 2 cut(s) 92, 804
BstSFI CTRYAG 1 cut(s) 624
BstSLI GKGCMC 1 cut(s) 734
BstUI CGCG 4 cut(s) 35, 759, 770, 772
BstV1I GCAGC 5 cut(s) 531, 610, 638, 794, 798
BstV2I GAAGAC 1 cut(s) 384
BstX2I RGATCY 1 cut(s) 206
BstXI CCANNNNNNTGG 1 cut(s) 253
BstYI RGATCY 1 cut(s) 206
Bsu15I ATCGAT 1 cut(s) 144
BsuRI GGCC 6 cut(s) 77, 201, 461, 470, 804, 863
BsuTUI ATCGAT 1 cut(s) 144
BtgI CCRYGG 3 cut(s) 57, 202, 297
BtsCI GGATG 4 cut(s) 262, 453, 632, 952
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 2 cut(s) 732, 828
BveI ACCTGC 1 cut(s) 515
Cac8I GCNNGC 3 cut(s) 75, 247, 325
CciI TCATGA 2 cut(s) 546, 648
CfoI GCGC 6 cut(s) 35, 281, 374, 524, 623, 772
Cfr10I RCCGGY 2 cut(s) 638, 695
Cfr13I GGNCC 2 cut(s) 260, 802
ClaI ATCGAT 1 cut(s) 144
CseI GACGC 1 cut(s) 53
Csp6I GTAC 2 cut(s) 699, 796
CspAI ACCGGT 1 cut(s) 638
CviAII CATG 7 cut(s) 58, 253, 334, 547, 649, 708, 955
CviQI GTAC 2 cut(s) 699, 796
DdeI CTNAG 1 cut(s) 579
DinI GGCGCC 2 cut(s) 280, 373
DpnI GATC 4 cut(s) 132, 183, 208, 836
DpnII GATC 4 cut(s) 130, 181, 206, 834
DraIII CACNNNGTG 1 cut(s) 96
EaeI YGGCCR 2 cut(s) 199, 459
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 3 cut(s) 57, 462, 610
Eco24I GRGCYC 2 cut(s) 159, 648
Eco31I GGTCTC 2 cut(s) 575, 730
Eco47I GGWCC 1 cut(s) 260
Eco53kI GAGCTC 1 cut(s) 157
EcoICRI GAGCTC 1 cut(s) 157
EcoO109I RGGNCCY 1 cut(s) 802
EcoRII CCWGG 1 cut(s) 804
EcoT14I CCWWGG 3 cut(s) 57, 462, 610
EcoT22I ATGCAT 1 cut(s) 709
EcoT38I GRGCYC 2 cut(s) 159, 648
EgeI GGCGCC 2 cut(s) 280, 373
EheI GGCGCC 2 cut(s) 280, 373
ErhI CCWWGG 3 cut(s) 57, 462, 610
Esp3I CGTCTC 1 cut(s) 262
FaeI CATG 7 cut(s) 61, 256, 337, 550, 652, 711, 958
FalI AAGNNNNNCTT 2 cut(s) 831, 863
FaqI GGGAC 2 cut(s) 241, 775
FatI CATG 7 cut(s) 57, 252, 333, 546, 648, 707, 954
FauI CCCGC 4 cut(s) 28, 332, 713, 752
FbaI TGATCA 1 cut(s) 834
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 8 cut(s) 31, 138, 277, 520, 624, 627, 783, 812
FokI GGATG 4 cut(s) 269, 460, 619, 959
FriOI GRGCYC 2 cut(s) 159, 648
Fsp4HI GCNGC 8 cut(s) 31, 138, 277, 520, 624, 627, 783, 812
GlaI GCGC 6 cut(s) 34, 280, 373, 523, 622, 771
GluI GCNGC 8 cut(s) 31, 138, 277, 520, 624, 627, 783, 812
GsuI CTGGAG 1 cut(s) 756
HaeII RGCGCY 2 cut(s) 282, 375
HaeIII GGCC 6 cut(s) 77, 201, 461, 470, 804, 863
HapII CCGG 5 cut(s) 94, 369, 639, 696, 864
HgaI GACGC 1 cut(s) 53
HhaI GCGC 6 cut(s) 35, 281, 374, 524, 623, 772
Hin1I GRCGYC 2 cut(s) 279, 372
Hin1II CATG 7 cut(s) 61, 256, 337, 550, 652, 711, 958
Hin6I GCGC 6 cut(s) 33, 279, 372, 522, 621, 770
HinP1I GCGC 6 cut(s) 33, 279, 372, 522, 621, 770
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 5 cut(s) 307, 496, 535, 677, 867
HpaII CCGG 5 cut(s) 94, 369, 639, 696, 864
HphI GGTGA 4 cut(s) 82, 208, 686, 902
Hpy166II GTNNAC 5 cut(s) 25, 43, 412, 571, 732
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 4 cut(s) 68, 167, 547, 649
Hpy8I GTNNAC 5 cut(s) 25, 43, 412, 571, 732
HpyAV CCTTC 2 cut(s) 196, 373
HpyCH4III ACNGT 4 cut(s) 232, 273, 301, 586
HpyCH4V TGCA 6 cut(s) 519, 626, 707, 732, 814, 821
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 218, 467
HpyF3I CTNAG 1 cut(s) 579
Hsp92I GRCGYC 2 cut(s) 279, 372
Hsp92II CATG 7 cut(s) 61, 256, 337, 550, 652, 711, 958
HspAI GCGC 6 cut(s) 33, 279, 372, 522, 621, 770
KasI GGCGCC 2 cut(s) 278, 371
KpnI GGTACC 1 cut(s) 702
Ksp22I TGATCA 1 cut(s) 834
Kzo9I GATC 4 cut(s) 130, 181, 206, 834
LmnI GCTCC 3 cut(s) 61, 70, 131
Lsp1109I GCAGC 5 cut(s) 531, 610, 638, 794, 798
LweI GCATC 4 cut(s) 120, 258, 438, 808
MaeIII GTNAC 2 cut(s) 674, 710
MalI GATC 4 cut(s) 132, 183, 208, 836
MbiI CCGCTC 2 cut(s) 126, 212
MboI GATC 4 cut(s) 130, 181, 206, 834
MboII GAAGA 2 cut(s) 181, 389
MfeI CAATTG 1 cut(s) 15
MflI RGATCY 1 cut(s) 206
MhlI GDGCHC 3 cut(s) 159, 648, 734
MlsI TGGCCA 1 cut(s) 461
MluCI AATT 5 cut(s) 15, 170, 455, 669, 910
MluNI TGGCCA 1 cut(s) 461
Mly113I GGCGCC 2 cut(s) 279, 372
MlyI GAGTC 3 cut(s) 544, 671, 861
Mox20I TGGCCA 1 cut(s) 461
Mph1103I ATGCAT 1 cut(s) 709
MscI TGGCCA 1 cut(s) 461
MseI TTAA 3 cut(s) 293, 303, 909
MslI CAYNNNNRTG 1 cut(s) 875
Msp20I TGGCCA 1 cut(s) 461
MspI CCGG 5 cut(s) 94, 369, 639, 696, 864
MspR9I CCNGG 2 cut(s) 94, 806
MunI CAATTG 1 cut(s) 15
MvaI CCWGG 1 cut(s) 806
MvnI CGCG 4 cut(s) 35, 759, 770, 772
MwoI GCNNNNNNNGC 3 cut(s) 70, 218, 467
NarI GGCGCC 2 cut(s) 279, 372
NciI CCSGG 1 cut(s) 94
NcoI CCATGG 1 cut(s) 57
NdeII GATC 4 cut(s) 130, 181, 206, 834
NlaIII CATG 7 cut(s) 61, 256, 337, 550, 652, 711, 958
NlaIV GGNNCC 7 cut(s) 98, 208, 262, 280, 373, 694, 700
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 1 cut(s) 674
NsiI ATGCAT 1 cut(s) 709
NspI RCATGY 1 cut(s) 711
PagI TCATGA 2 cut(s) 546, 648
PaqCI CACCTGC 1 cut(s) 515
PcsI WCGNNNNNNNCGW 3 cut(s) 141, 150, 327
PfeI GAWTC 2 cut(s) 307, 496
PflMI CCANNNNNTGG 1 cut(s) 689
PinAI ACCGGT 1 cut(s) 638
PkrI GCNGC 8 cut(s) 32, 139, 278, 521, 625, 628, 784, 813
PleI GAGTC 3 cut(s) 543, 671, 861
PluTI GGCGCC 2 cut(s) 282, 375
PpsI GAGTC 3 cut(s) 543, 671, 861
Psp124BI GAGCTC 1 cut(s) 159
Psp6I CCWGG 1 cut(s) 804
PspGI CCWGG 1 cut(s) 804
PspN4I GGNNCC 7 cut(s) 98, 208, 262, 280, 373, 694, 700
PspPI GGNCC 2 cut(s) 260, 802
PstI CTGCAG 1 cut(s) 628
PsuI RGATCY 1 cut(s) 206
RsaI GTAC 2 cut(s) 700, 797
RsaNI GTAC 2 cut(s) 699, 796
RseI CAYNNNNRTG 1 cut(s) 875
SacI GAGCTC 1 cut(s) 159
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 3 cut(s) 293, 303, 909
SatI GCNGC 8 cut(s) 31, 138, 277, 520, 624, 627, 783, 812
Sau3AI GATC 4 cut(s) 130, 181, 206, 834
Sau96I GGNCC 2 cut(s) 260, 802
SchI GAGTC 3 cut(s) 544, 671, 861
ScrFI CCNGG 2 cut(s) 94, 806
SduI GDGCHC 3 cut(s) 159, 648, 734
SfaNI GCATC 4 cut(s) 120, 258, 438, 808
SfcI CTRYAG 1 cut(s) 624
SfiI GGCCNNNNNGGCC 1 cut(s) 467
SfoI GGCGCC 2 cut(s) 280, 373
SgrAI CRCCGGYG 1 cut(s) 638
SinI GGWCC 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 875
Sse9I AATT 5 cut(s) 15, 170, 455, 669, 910
SspDI GGCGCC 2 cut(s) 278, 371
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 2 cut(s) 92, 804
StyI CCWWGG 3 cut(s) 57, 462, 610
TaaI ACNGT 4 cut(s) 232, 273, 301, 586
TaqI TCGA 4 cut(s) 42, 144, 153, 667
TasI AATT 5 cut(s) 15, 170, 455, 669, 910
TatI WGTACW 1 cut(s) 795
TauI GCSGC 3 cut(s) 33, 140, 279
TfiI GAWTC 2 cut(s) 307, 496
Tru1I TTAA 3 cut(s) 293, 303, 909
Tru9I TTAA 3 cut(s) 293, 303, 909
TscAI CASTG 2 cut(s) 739, 828
TseFI GTSAC 1 cut(s) 674
TseI GCWGC 5 cut(s) 519, 623, 626, 782, 811
Tsp45I GTSAC 1 cut(s) 674
TspDTI ATGAA 4 cut(s) 415, 563, 665, 943
TspGWI ACGGA 2 cut(s) 446, 528
TspRI CASTG 2 cut(s) 739, 828
Van91I CCANNNNNTGG 1 cut(s) 689
VneI GTGCAC 1 cut(s) 730
VpaK11BI GGWCC 1 cut(s) 260
XceI RCATGY 1 cut(s) 711
XmiI GTMKAC 1 cut(s) 42
Zsp2I ATGCAT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.