pycom08g09790

Aspartate aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011825
Physical Location & Seq
Forward (+)
8131954 .. 8135095
3142 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1227 bp
ATGGAGTCCCAAGCCGGCGGTTCCGTATTCTCCCACGTCCCTCAGGTCCCTGAAGTCCCACTTTACATGCAGGCTGCTTATGGGAAAGACTCGAGTCCTCAGAAGCTGAATTTGGGCATTGGTGTTTACAGAACTGAGGATGGGAGACCTGTCCTTTTAGATGTGGTTAGTCGAGCGGAGCAGTTACTAGTCAATGACTTGTCCAGTTTTAAGGATTATCTTCCTATTACTGGAATGCCAGATTTCAACAGATTGAGCGCTGCACTCGTTTTGGGTGCTGACAGCCCTGCTATAAAGGAGAATAGAGTGACTACTGCCCAGTGCTTGTCTGGTAGCGGTTCAATAAGGATTGGAGCCGATTTTTTAGCAAAGCATTACGGTCAACATCATATAGTGTACATGTCCCAGCAAACATATGGAAACCACCCAAATTTTTTCTCCGCGGCAGGGATAGCTATTAAGACTTACCGTTACTATGACCCAGGAACGCGTGGACTGGATTTTCAAGGCATGCTGGAAGATCTTCGTTCTTCCCCAACAGGAGCTATCGTGCTTCTCCAAGCATGTGCGCAAAATCCCACTGGTGTTGATCCAACTATGTCACAGTGGAAGCAGATAAGACAGTTGATCAGATCAAAAGGGTTGTTACCGTTTTTTGACTGTGCTTATCAGGGTTTTGTCAGTGGAAATCTGGAAGAAGATGCGCAATCGATTCGCCTTTTTGTTGCTGATGGTGGTGAATGCCTTATAGCCCAGTCATATTCAAAGAATATGGGACTTTACGGAGAACGTGTTGGGGCACTCAGCATTGTTTGCAAGACAGCAGAAGTGGCCTGCAACGTCGAAAGCCAGCTGAAACTCGTGATCAGGCCCATGTATTCTAACCCACCCATTCATGGGGCAGCCATAGTGGCTGCTATCCTAAAGGACAGGTACAATGAATGGAGTATTGAAGTGAAGGCAATGGCTGACCGCCTAATGAGCATGCGCCAACACCTATTCAATGCGCTGCGTGACAAAGGTACTCCCGGTGACTGGAGTTACATAATCAGGCAAGTGGGGATGTTTACTTTCTCAGGATTGAGCCCTGAACAAGTTGCCTTCATTACAAAAGAGTACCACATCTACTTGCCATCTGATGGGAGGATTAACATGGCAGGTCTTGGTCCTAAGACAGTTCCTCATCTTGCAGATGCAATACACGCAGCTGTAACCACTGCTGTCTAA

Protein Analysis

409

Amino Acids

44.62

Weight (kDa)

6.79

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 34 - 400 1.2e-85 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011825)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62800 AT1G62800 AT1G62800 AT5G19550
fragaria_vesca FvH4_2g36020
malus_domestica MD08G1117300.v1.1
prunus_persica Prupe.1G451800_v2.0.a1 Prupe.1G451800_v2.0.a1
pyrus_communis pycom08g09790
rosa_chinensis RchiOBHm_Chr6g0305831
rosa_laevigata RLG00000010854
rosa_roxburghii Rroxscaffold_7G00162290
rosa_rugosa Rorug06G0344000 Rorug06G0344100.1 Rorug06G0344200
rosa_samantha Rh6AG456000 Rh6BG445100 Rh6CG470000
rosa_wichuraiana Rw6G039790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 570, 705
Acc36I ACCTGC 1 cut(s) 1148
AccB7I CCANNNNNTGG 1 cut(s) 1139
AccBSI CCGCTC 1 cut(s) 176
AccII CGCG 2 cut(s) 443, 490
AciI CCGC 6 cut(s) 18, 176, 336, 441, 443, 973
AclWI GGATC 1 cut(s) 584
AcsI RAATTY 2 cut(s) 109, 430
AcuI CTGAAG 1 cut(s) 72
AfaI GTAC 4 cut(s) 398, 935, 1024, 1118
AfeI AGCGCT 1 cut(s) 259
AfiI CCNNNNNNNGG 6 cut(s) 230, 447, 896, 897, 1035, 1139
AflIII ACRYGT 3 cut(s) 399, 488, 790
AgsI TTSAA 6 cut(s) 247, 342, 506, 765, 953, 1003
AhlI ACTAGT 1 cut(s) 187
AjiI CACGTC 1 cut(s) 37
AjnI CCWGG 1 cut(s) 481
AjuI GAANNNNNNNTTGG 2 cut(s) 95, 127
AluBI AGCT 5 cut(s) 106, 455, 545, 853, 1208
AluI AGCT 5 cut(s) 106, 455, 545, 853, 1208
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 1 cut(s) 584
AlwNI CAGNNNCTG 1 cut(s) 106
Ama87I CYCGRG 1 cut(s) 91
Aor51HI AGCGCT 1 cut(s) 259
AoxI GGCC 2 cut(s) 831, 869
ApeKI GCWGC 6 cut(s) 74, 260, 902, 914, 1009, 1205
ApoI RAATTY 2 cut(s) 109, 430
Asp700I GAANNNNTTC 1 cut(s) 522
AspLEI GCGC 5 cut(s) 260, 571, 706, 990, 1009
AspS9I GGNCC 3 cut(s) 46, 870, 1166
AsuC2I CCSGG 1 cut(s) 1029
AsuHPI GGTGA 2 cut(s) 749, 1043
AvaI CYCGRG 1 cut(s) 91
AvaII GGWCC 2 cut(s) 46, 1166
AxyI CCTNAGG 1 cut(s) 42
BaeGI GKGCMC 1 cut(s) 802
BanII GRGCYC 1 cut(s) 1088
BauI CACGAG 1 cut(s) 860
BbvI GCAGC 6 cut(s) 61, 247, 901, 914, 996, 1217
BccI CCATC 4 cut(s) 134, 725, 1133, 1141
BcgI CGANNNNNNTGC 2 cut(s) 695, 729
BciT130I CCWGG 1 cut(s) 483
BclI TGATCA 2 cut(s) 627, 864
BcnI CCSGG 1 cut(s) 1029
BcoDI GTCTC 1 cut(s) 139
BcuI ACTAGT 1 cut(s) 187
BfaI CTAG 1 cut(s) 188
BfoI RGCGCY 1 cut(s) 261
BfuAI ACCTGC 1 cut(s) 1148
BglI GCCNNNNNGGC 1 cut(s) 911
BglII AGATCT 1 cut(s) 520
BisI GCNGC 7 cut(s) 75, 261, 444, 903, 915, 1010, 1206
BlsI GCNGC 7 cut(s) 76, 262, 445, 904, 916, 1011, 1207
Bme1390I CCNGG 2 cut(s) 483, 1029
Bme18I GGWCC 2 cut(s) 46, 1166
BmeT110I CYCGRG 1 cut(s) 91
BmgBI CACGTC 1 cut(s) 37
BmgT120I GGNCC 3 cut(s) 46, 870, 1166
BmiI GGNNCC 3 cut(s) 22, 48, 355
BmrFI CCNGG 2 cut(s) 483, 1029
BmrI ACTGGG 2 cut(s) 313, 748
BmsI GCATC 2 cut(s) 691, 1183
BmuI ACTGGG 2 cut(s) 313, 748
BoxI GACNNNNGTC 1 cut(s) 93
BpmI CTGGAG 1 cut(s) 1057
BpuMI CCSGG 1 cut(s) 1029
Bsa29I ATCGAT 1 cut(s) 710
BsaI GGTCTC 1 cut(s) 139
BsaJI CCNNGG 2 cut(s) 441, 481
BsaXI ACNNNNNCTCC 4 cut(s) 534, 564, 777, 807
Bsc4I CCNNNNNNNGG 6 cut(s) 230, 447, 896, 897, 1035, 1139
Bse118I RCCGGY 1 cut(s) 14
Bse1I ACTGG 7 cut(s) 204, 235, 319, 501, 586, 754, 1040
Bse21I CCTNAGG 1 cut(s) 42
Bse3DI GCAATG 1 cut(s) 969
BseBI CCWGG 1 cut(s) 483
BseCI ATCGAT 1 cut(s) 710
BseDI CCNNGG 2 cut(s) 441, 481
BseGI GGATG 2 cut(s) 145, 1068
BseLI CCNNNNNNNGG 6 cut(s) 230, 447, 896, 897, 1035, 1139
BseMI GCAATG 1 cut(s) 969
BseMII CTCAG 5 cut(s) 56, 113, 126, 817, 1089
BseNI ACTGG 7 cut(s) 204, 235, 319, 501, 586, 754, 1040
BseSI GKGCMC 1 cut(s) 802
BseXI GCAGC 6 cut(s) 61, 247, 901, 914, 996, 1217
BseYI CCCAGC 1 cut(s) 405
BsgI GTGCAG 1 cut(s) 246
Bsh1236I CGCG 2 cut(s) 443, 490
BshFI GGCC 2 cut(s) 833, 871
BshVI ATCGAT 1 cut(s) 710
BsiHKCI CYCGRG 1 cut(s) 91
BsiSI CCGG 2 cut(s) 15, 1029
BslFI GGGAC 5 cut(s) 23, 32, 41, 388, 789
BslI CCNNNNNNNGG 6 cut(s) 230, 447, 896, 897, 1035, 1139
BsmAI GTCTC 1 cut(s) 139
BsmFI GGGAC 5 cut(s) 23, 32, 41, 388, 789
BsmI GAATGC 2 cut(s) 240, 746
BsnI GGCC 2 cut(s) 833, 871
Bso31I GGTCTC 1 cut(s) 139
BsoBI CYCGRG 1 cut(s) 91
Bsp1286I GDGCHC 2 cut(s) 802, 1088
Bsp1407I TGTACA 1 cut(s) 396
Bsp143I GATC 5 cut(s) 520, 589, 627, 632, 864
BspACI CCGC 6 cut(s) 18, 176, 336, 441, 443, 973
BspANI GGCC 2 cut(s) 833, 871
BspCNI CTCAG 5 cut(s) 55, 112, 127, 816, 1088
BspDI ATCGAT 1 cut(s) 710
BspFNI CGCG 2 cut(s) 443, 490
BspLI GGNNCC 3 cut(s) 22, 48, 355
BspMI ACCTGC 1 cut(s) 1148
BspPI GGATC 1 cut(s) 584
BspTNI GGTCTC 1 cut(s) 139
BsrBI CCGCTC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 969
BsrFI RCCGGY 1 cut(s) 14
BsrGI TGTACA 1 cut(s) 396
BsrI ACTGG 7 cut(s) 204, 235, 319, 501, 586, 754, 1040
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 2 cut(s) 441, 481
BssMI GATC 5 cut(s) 520, 589, 627, 632, 864
BssSI CACGAG 1 cut(s) 860
Bst2BI CACGAG 1 cut(s) 860
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 7 cut(s) 380, 470, 606, 624, 651, 662, 1177
BstAPI GCANNNNNTGC 1 cut(s) 813
BstAUI TGTACA 1 cut(s) 396
BstC8I GCNNGC 6 cut(s) 16, 72, 512, 835, 851, 986
BstDEI CTNAG 6 cut(s) 42, 99, 135, 803, 1075, 1170
BstDSI CCRYGG 1 cut(s) 441
BstF5I GGATG 2 cut(s) 145, 1068
BstFNI CGCG 2 cut(s) 443, 490
BstH2I RGCGCY 1 cut(s) 261
BstHHI GCGC 5 cut(s) 260, 571, 706, 990, 1009
BstKTI GATC 5 cut(s) 523, 592, 630, 635, 867
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 5 cut(s) 520, 589, 627, 632, 864
BstMWI GCNNNNNNNGC 6 cut(s) 452, 813, 830, 911, 981, 1202
BstNI CCWGG 1 cut(s) 483
BstNSI RCATGY 5 cut(s) 70, 403, 514, 567, 988
BstPAI GACNNNNGTC 1 cut(s) 93
BstSCI CCNGG 2 cut(s) 481, 1027
BstSLI GKGCMC 1 cut(s) 802
BstUI CGCG 2 cut(s) 443, 490
BstV1I GCAGC 6 cut(s) 61, 247, 901, 914, 996, 1217
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
Bsu15I ATCGAT 1 cut(s) 710
Bsu36I CCTNAGG 1 cut(s) 42
BsuRI GGCC 2 cut(s) 833, 871
BsuTUI ATCGAT 1 cut(s) 710
BtgI CCRYGG 1 cut(s) 441
BtrI CACGTC 1 cut(s) 37
BtsCI GGATG 2 cut(s) 145, 1068
BtsI GCAGTG 1 cut(s) 1215
BtsIMutI CAGTG 5 cut(s) 326, 579, 611, 688, 1215
BveI ACCTGC 1 cut(s) 1148
Cac8I GCNNGC 6 cut(s) 16, 72, 512, 835, 851, 986
CaiI CAGNNNCTG 1 cut(s) 106
CfoI GCGC 5 cut(s) 260, 571, 706, 990, 1009
Cfr10I RCCGGY 1 cut(s) 14
Cfr13I GGNCC 3 cut(s) 46, 870, 1166
Cfr42I CCGCGG 1 cut(s) 444
ClaI ATCGAT 1 cut(s) 710
Csp6I GTAC 4 cut(s) 397, 934, 1023, 1117
CspCI CAANNNNNGTGG 2 cut(s) 568, 603
CviAII CATG 8 cut(s) 67, 400, 511, 564, 874, 896, 985, 1153
CviQI GTAC 4 cut(s) 397, 934, 1023, 1117
DdeI CTNAG 6 cut(s) 42, 99, 135, 803, 1075, 1170
DpnI GATC 5 cut(s) 522, 591, 629, 634, 866
DpnII GATC 5 cut(s) 520, 589, 627, 632, 864
Eco24I GRGCYC 1 cut(s) 1088
Eco31I GGTCTC 1 cut(s) 139
Eco47I GGWCC 2 cut(s) 46, 1166
Eco47III AGCGCT 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 72
Eco81I CCTNAGG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 91
EcoO109I RGGNCCY 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 481
EcoT38I GRGCYC 1 cut(s) 1088
FaeI CATG 8 cut(s) 70, 403, 514, 567, 877, 899, 988, 1156
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 5 cut(s) 23, 32, 41, 388, 789
FatI CATG 8 cut(s) 66, 399, 510, 563, 873, 895, 984, 1152
FauNDI CATATG 1 cut(s) 415
FbaI TGATCA 2 cut(s) 627, 864
Fnu4HI GCNGC 7 cut(s) 75, 261, 444, 903, 915, 1010, 1206
FokI GGATG 2 cut(s) 152, 1075
FriOI GRGCYC 1 cut(s) 1088
Fsp4HI GCNGC 7 cut(s) 75, 261, 444, 903, 915, 1010, 1206
FspBI CTAG 1 cut(s) 188
FspI TGCGCA 2 cut(s) 570, 705
GlaI GCGC 5 cut(s) 259, 570, 705, 989, 1008
GluI GCNGC 7 cut(s) 75, 261, 444, 903, 915, 1010, 1206
GsaI CCCAGC 1 cut(s) 409
GsuI CTGGAG 1 cut(s) 1057
HaeII RGCGCY 1 cut(s) 261
HaeIII GGCC 2 cut(s) 833, 871
HapII CCGG 2 cut(s) 15, 1029
HhaI GCGC 5 cut(s) 260, 571, 706, 990, 1009
Hin1II CATG 8 cut(s) 70, 403, 514, 567, 877, 899, 988, 1156
Hin6I GCGC 5 cut(s) 258, 569, 704, 988, 1007
HinP1I GCGC 5 cut(s) 258, 569, 704, 988, 1007
HincII GTYRAC 1 cut(s) 383
HindII GTYRAC 1 cut(s) 383
HinfI GANTC 4 cut(s) 5, 89, 94, 712
HpaII CCGG 2 cut(s) 15, 1029
HphI GGTGA 2 cut(s) 749, 1043
Hpy166II GTNNAC 5 cut(s) 127, 383, 397, 494, 1068
Hpy188I TCNGA 3 cut(s) 102, 632, 1138
Hpy188III TCNNGA 3 cut(s) 692, 862, 1077
Hpy8I GTNNAC 5 cut(s) 127, 383, 397, 494, 1068
Hpy99I CGWCG 1 cut(s) 845
HpyAV CCTTC 2 cut(s) 952, 1111
HpyCH4III ACNGT 7 cut(s) 380, 470, 606, 624, 651, 662, 1177
HpyCH4IV ACGT 3 cut(s) 36, 790, 840
HpyCH4V TGCA 6 cut(s) 70, 263, 816, 837, 1190, 1196
HpyF10VI GCNNNNNNNGC 6 cut(s) 452, 813, 830, 911, 981, 1202
HpyF3I CTNAG 6 cut(s) 42, 99, 135, 803, 1075, 1170
HpySE526I ACGT 3 cut(s) 36, 790, 840
Hsp92II CATG 8 cut(s) 70, 403, 514, 567, 877, 899, 988, 1156
HspAI GCGC 5 cut(s) 258, 569, 704, 988, 1007
KroI GCCGGC 1 cut(s) 14
KroNI GCCGGC 1 cut(s) 16
Ksp22I TGATCA 2 cut(s) 627, 864
KspI CCGCGG 1 cut(s) 444
Kzo9I GATC 5 cut(s) 520, 589, 627, 632, 864
LmnI GCTCC 3 cut(s) 178, 353, 542
Lsp1109I GCAGC 6 cut(s) 61, 247, 901, 914, 996, 1217
LweI GCATC 2 cut(s) 691, 1183
MaeI CTAG 1 cut(s) 188
MaeII ACGT 3 cut(s) 36, 790, 840
MaeIII GTNAC 9 cut(s) 183, 307, 470, 600, 645, 1013, 1031, 1040, 1210
MalI GATC 5 cut(s) 522, 591, 629, 634, 866
MbiI CCGCTC 1 cut(s) 176
MboI GATC 5 cut(s) 520, 589, 627, 632, 864
MboII GAAGA 6 cut(s) 212, 515, 522, 530, 707, 710
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 2 cut(s) 802, 1088
MluCI AATT 2 cut(s) 109, 430
MluI ACGCGT 1 cut(s) 488
MlyI GAGTC 3 cut(s) 14, 83, 103
MmeI TCCRAC 1 cut(s) 617
MnlI CCTC 5 cut(s) 51, 108, 130, 1137, 1191
MroNI GCCGGC 1 cut(s) 14
MroXI GAANNNNTTC 1 cut(s) 522
MseI TTAA 3 cut(s) 210, 459, 1149
MspA1I CMGCKG 3 cut(s) 443, 853, 1208
MspI CCGG 2 cut(s) 15, 1029
MspR9I CCNGG 2 cut(s) 483, 1029
Mva1269I GAATGC 2 cut(s) 240, 746
MvaI CCWGG 1 cut(s) 483
MvnI CGCG 2 cut(s) 443, 490
MwoI GCNNNNNNNGC 6 cut(s) 452, 813, 830, 911, 981, 1202
NaeI GCCGGC 1 cut(s) 16
NciI CCSGG 1 cut(s) 1029
NdeI CATATG 1 cut(s) 415
NdeII GATC 5 cut(s) 520, 589, 627, 632, 864
NgoMIV GCCGGC 1 cut(s) 14
NlaIII CATG 8 cut(s) 70, 403, 514, 567, 877, 899, 988, 1156
NlaIV GGNNCC 3 cut(s) 22, 48, 355
NmuCI GTSAC 4 cut(s) 307, 600, 1013, 1031
NsbI TGCGCA 2 cut(s) 570, 705
NspI RCATGY 5 cut(s) 70, 403, 514, 567, 988
PaeI GCATGC 2 cut(s) 514, 988
PaeR7I CTCGAG 1 cut(s) 91
PciI ACATGT 1 cut(s) 399
PctI GAATGC 2 cut(s) 240, 746
PdiI GCCGGC 1 cut(s) 16
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 1 cut(s) 712
PflMI CCANNNNNTGG 1 cut(s) 1139
PkrI GCNGC 7 cut(s) 76, 262, 445, 904, 916, 1011, 1207
PleI GAGTC 3 cut(s) 13, 83, 102
PpsI GAGTC 3 cut(s) 13, 83, 102
PpuMI RGGWCCY 1 cut(s) 46
PscI ACATGT 1 cut(s) 399
PshAI GACNNNNGTC 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 46
Psp6I CCWGG 1 cut(s) 481
PspFI CCCAGC 1 cut(s) 405
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 3 cut(s) 22, 48, 355
PspPI GGNCC 3 cut(s) 46, 870, 1166
PspPPI RGGWCCY 1 cut(s) 46
PspXI VCTCGAGB 1 cut(s) 91
PstNI CAGNNNCTG 1 cut(s) 106
PsuI RGATCY 1 cut(s) 520
PvuII CAGCTG 2 cut(s) 853, 1208
RsaI GTAC 4 cut(s) 398, 935, 1024, 1118
RsaNI GTAC 4 cut(s) 397, 934, 1023, 1117
SacII CCGCGG 1 cut(s) 444
SaqAI TTAA 3 cut(s) 210, 459, 1149
SatI GCNGC 7 cut(s) 75, 261, 444, 903, 915, 1010, 1206
Sau3AI GATC 5 cut(s) 520, 589, 627, 632, 864
Sau96I GGNCC 3 cut(s) 46, 870, 1166
SchI GAGTC 3 cut(s) 14, 83, 103
ScrFI CCNGG 2 cut(s) 483, 1029
SduI GDGCHC 2 cut(s) 802, 1088
SfaNI GCATC 2 cut(s) 691, 1183
Sfr274I CTCGAG 1 cut(s) 91
Sfr303I CCGCGG 1 cut(s) 444
SgrBI CCGCGG 1 cut(s) 444
SinI GGWCC 2 cut(s) 46, 1166
SlaI CTCGAG 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 91
SmoI CTYRAG 1 cut(s) 91
SpeI ACTAGT 1 cut(s) 187
SphI GCATGC 2 cut(s) 514, 988
Sse9I AATT 2 cut(s) 109, 430
SsiI CCGC 6 cut(s) 18, 176, 336, 441, 443, 973
SspMI CTAG 1 cut(s) 188
StyD4I CCNGG 2 cut(s) 481, 1027
TaaI ACNGT 7 cut(s) 380, 470, 606, 624, 651, 662, 1177
TaiI ACGT 3 cut(s) 39, 793, 843
TaqI TCGA 4 cut(s) 92, 172, 710, 843
TasI AATT 2 cut(s) 109, 430
TatI WGTACW 1 cut(s) 396
TauI GCSGC 1 cut(s) 446
TfiI GAWTC 1 cut(s) 712
Tru1I TTAA 3 cut(s) 210, 459, 1149
Tru9I TTAA 3 cut(s) 210, 459, 1149
TscAI CASTG 5 cut(s) 326, 586, 611, 688, 1222
TseFI GTSAC 4 cut(s) 307, 600, 1013, 1031
TseI GCWGC 6 cut(s) 74, 260, 902, 914, 1009, 1205
Tsp45I GTSAC 4 cut(s) 307, 600, 1013, 1031
TspDTI ATGAA 3 cut(s) 884, 954, 1093
TspGWI ACGGA 2 cut(s) 13, 798
TspRI CASTG 5 cut(s) 326, 586, 611, 688, 1222
Van91I CCANNNNNTGG 1 cut(s) 1139
VpaK11BI GGWCC 2 cut(s) 46, 1166
XapI RAATTY 2 cut(s) 109, 430
XceI RCATGY 5 cut(s) 70, 403, 514, 567, 988
XcmI CCANNNNNNNNNTGG 2 cut(s) 326, 413
XhoI CTCGAG 1 cut(s) 91
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.