Rw6G039790

Aspartate aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0011825
Physical Location & Seq
Reverse (-)
63690948 .. 63694730
3783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G039790.1

Sequence Viewer

Length: 1062 bp
ATGGAGCCACAAGAAGGCGATTCTGTATTCGCACACATTCCTCACGTTCCTGAAGTCCCAATTTACGCGATGATGGTTGCTTTCAGCAGAGACCCAAGTCCACTGAAGCTCAATTTAGGGATTGGTGTCTATAGAACTGAGGATGGGAAACCTTTTCAATTGAATGTAGTTAGACGAGTGGAGCGGTTACTAGTTAATGACAGGTCCACTTTTAAGGAGTATCTTCCTATTACTGGGACTGCTGACTTCAACAGATTGAGTGCTAGGCTCATTTTGGGTGCTGACTGCCCTGCTATCAAGGAGAATAGAGTCACTACTGTCCAGTGCTTATCTGGTAGTGGATCACTGAGGGTCGGAGCTGAGTTTCTAGCAAACCATTACCACCATCAGCATACGGTTTACATGTCCCAGCCAACATATGCAGCCCACCCGGCTTTTTTCTCTGCAGCAGGGCTAGCTATCAAGACTTACCGTTACTATGACCCAGGAACATGTGGACTGGACTTCCAAGGAATGTTAGAAGATCTTTTGTCTGCCCCATCAGGAGCTATTGTGCTTCTCCAAGCATGTGCACATAACCCTACAGGTGTTGATCCAACTCTTCCACAGTGGGAGCAGATAAGACAGTTGATTAGAGCAAAGGGATTGTTTCCTTTCTTTGACTCTGCTTATCAGGGTCTTGTCAGTGGAAATCTGGATGAGGATGCACAATTTATTCGCCTGTTTGTTGCTGATGGTGGTGAGTGCCTTATAGCTCAGTCATATTCGAAGATTATGGGACTTTATGGAGAACGTGTTGGTGCCCTCAGCATTGTTTGCAAGACAGCAGAAGTGGCTAGCCATGTTGAAAGCCAGCTGAAACTTGTGATCAGGCCCATGTATTCTAGCCCCCCAATTCATGGGGCATCCATAGTGGCCACTATCTTAAAGGATAGGGATATGTATGAGGAATGGAGTATTGAAGTGAAGGCAATGACTGACCGCATTATTAGCACGCGTAGACAACTATTTGATGCTCTGCGTGATAGAGGCAATACAATCTCTCTTTCTGTACTCTTCTAG

Protein Analysis

353

Amino Acids

39.1

Weight (kDa)

6.21

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 36 - 344 5.2e-75 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011825)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G62800 AT1G62800 AT1G62800 AT5G19550
fragaria_vesca FvH4_2g36020
malus_domestica MD08G1117300.v1.1
prunus_persica Prupe.1G451800_v2.0.a1 Prupe.1G451800_v2.0.a1
pyrus_communis pycom08g09790
rosa_chinensis RchiOBHm_Chr6g0305831
rosa_laevigata RLG00000010854
rosa_roxburghii Rroxscaffold_7G00162290
rosa_rugosa Rorug06G0344000 Rorug06G0344100.1 Rorug06G0344200
rosa_samantha Rh6AG456000 Rh6BG445100 Rh6CG470000
rosa_wichuraiana Rw6G039790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 800
AccB7I CCANNNNNTGG 1 cut(s) 899
AccBSI CCGCTC 1 cut(s) 184
AccI GTMKAC 1 cut(s) 1000
AccII CGCG 2 cut(s) 68, 997
AciI CCGC 2 cut(s) 184, 982
AclWI GGATC 2 cut(s) 349, 587
AcoI YGGCCR 1 cut(s) 915
AcuI CTGAAG 2 cut(s) 72, 125
AfaI GTAC 1 cut(s) 1053
AfiI CCNNNNNNNGG 3 cut(s) 14, 233, 899
AflIII ACRYGT 4 cut(s) 402, 491, 793, 995
AgsI TTSAA 5 cut(s) 158, 163, 250, 848, 962
AhlI ACTAGT 1 cut(s) 190
AjnI CCWGG 1 cut(s) 484
AluBI AGCT 6 cut(s) 109, 359, 458, 548, 755, 856
AluI AGCT 6 cut(s) 109, 359, 458, 548, 755, 856
Alw21I GWGCWC 1 cut(s) 574
Alw26I GTCTC 1 cut(s) 84
Alw44I GTGCAC 1 cut(s) 570
AlwI GGATC 2 cut(s) 349, 587
AoxI GGCC 2 cut(s) 872, 915
ApaLI GTGCAC 1 cut(s) 570
ApeKI GCWGC 2 cut(s) 422, 446
AspS9I GGNCC 2 cut(s) 204, 873
AsuC2I CCSGG 1 cut(s) 431
AsuHPI GGTGA 1 cut(s) 752
AsuII TTCGAA 1 cut(s) 767
AsuNHI GCTAGC 2 cut(s) 454, 836
AvaII GGWCC 1 cut(s) 204
BaeGI GKGCMC 2 cut(s) 574, 805
BalI TGGCCA 1 cut(s) 917
BanI GGYRCC 1 cut(s) 800
Bbv12I GWGCWC 1 cut(s) 574
BbvCI CCTCAGC 1 cut(s) 806
BbvI GCAGC 2 cut(s) 434, 458
BccI CCATC 5 cut(s) 67, 137, 393, 547, 728
BciT130I CCWGG 1 cut(s) 486
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 1 cut(s) 431
BcoDI GTCTC 1 cut(s) 84
BcuI ACTAGT 1 cut(s) 190
BfaI CTAG 7 cut(s) 191, 264, 368, 455, 837, 885, 1060
BfmI CTRYAG 3 cut(s) 130, 444, 582
BglI GCCNNNNNGGC 1 cut(s) 431
BglII AGATCT 1 cut(s) 523
BisI GCNGC 2 cut(s) 423, 447
BlsI GCNGC 2 cut(s) 424, 448
Bme1390I CCNGG 2 cut(s) 431, 486
Bme18I GGWCC 1 cut(s) 204
BmgT120I GGNCC 2 cut(s) 204, 873
BmiI GGNNCC 2 cut(s) 6, 802
BmrFI CCNGG 2 cut(s) 431, 486
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 3 cut(s) 694, 914, 1003
BmtI GCTAGC 2 cut(s) 458, 840
BmuI ACTGGG 1 cut(s) 243
BoxI GACNNNNGTC 1 cut(s) 96
Bpu10I CCTNAGC 1 cut(s) 806
Bpu14I TTCGAA 1 cut(s) 767
BpuMI CCSGG 1 cut(s) 431
BsaI GGTCTC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 484, 508
BsaXI ACNNNNNCTCC 4 cut(s) 537, 567, 780, 810
Bsc4I CCNNNNNNNGG 3 cut(s) 14, 233, 899
Bse1I ACTGG 3 cut(s) 238, 322, 504
Bse3DI GCAATG 1 cut(s) 978
BseBI CCWGG 1 cut(s) 486
BseDI CCNNGG 2 cut(s) 484, 508
BseGI GGATG 4 cut(s) 148, 703, 709, 905
BseLI CCNNNNNNNGG 3 cut(s) 14, 233, 899
BseMI GCAATG 1 cut(s) 978
BseMII CTCAG 5 cut(s) 129, 338, 351, 770, 820
BseNI ACTGG 3 cut(s) 238, 322, 504
BseSI GKGCMC 2 cut(s) 574, 805
BseXI GCAGC 2 cut(s) 434, 458
BseYI CCCAGC 1 cut(s) 408
Bsh1236I CGCG 2 cut(s) 68, 997
BshFI GGCC 2 cut(s) 874, 917
BshNI GGYRCC 1 cut(s) 800
BsiHKAI GWGCWC 1 cut(s) 574
BsiSI CCGG 1 cut(s) 431
BslFI GGGAC 4 cut(s) 41, 250, 391, 792
BslI CCNNNNNNNGG 3 cut(s) 14, 233, 899
BsmAI GTCTC 1 cut(s) 84
BsmFI GGGAC 4 cut(s) 41, 250, 391, 792
BsnI GGCC 2 cut(s) 874, 917
Bso31I GGTCTC 1 cut(s) 84
Bsp119I TTCGAA 1 cut(s) 767
Bsp1286I GDGCHC 2 cut(s) 574, 805
Bsp143I GATC 4 cut(s) 341, 523, 592, 867
BspACI CCGC 2 cut(s) 184, 982
BspANI GGCC 2 cut(s) 874, 917
BspCNI CTCAG 5 cut(s) 130, 339, 352, 769, 819
BspFNI CGCG 2 cut(s) 68, 997
BspLI GGNNCC 2 cut(s) 6, 802
BspMAI CTGCAG 1 cut(s) 448
BspOI GCTAGC 2 cut(s) 458, 840
BspPI GGATC 2 cut(s) 349, 587
BspT104I TTCGAA 1 cut(s) 767
BspT107I GGYRCC 1 cut(s) 800
BspTNI GGTCTC 1 cut(s) 84
BsrBI CCGCTC 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 978
BsrI ACTGG 3 cut(s) 238, 322, 504
BssECI CCNNGG 2 cut(s) 484, 508
BssMI GATC 4 cut(s) 341, 523, 592, 867
BssT1I CCWWGG 1 cut(s) 508
Bst2UI CCWGG 1 cut(s) 486
Bst4CI ACNGT 5 cut(s) 319, 397, 473, 609, 627
Bst6I CTCTTC 1 cut(s) 606
BstAPI GCANNNNNTGC 1 cut(s) 816
BstBI TTCGAA 1 cut(s) 767
BstC8I GCNNGC 4 cut(s) 456, 838, 854, 995
BstDEI CTNAG 5 cut(s) 138, 347, 360, 756, 806
BstF5I GGATG 4 cut(s) 148, 703, 709, 905
BstFNI CGCG 2 cut(s) 68, 997
BstKTI GATC 4 cut(s) 344, 526, 595, 870
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 4 cut(s) 341, 523, 592, 867
BstMWI GCNNNNNNNGC 5 cut(s) 431, 455, 816, 833, 990
BstNI CCWGG 1 cut(s) 486
BstNSI RCATGY 3 cut(s) 406, 495, 570
BstPAI GACNNNNGTC 1 cut(s) 96
BstSCI CCNGG 2 cut(s) 429, 484
BstSFI CTRYAG 3 cut(s) 130, 444, 582
BstSLI GKGCMC 2 cut(s) 574, 805
BstUI CGCG 2 cut(s) 68, 997
BstV1I GCAGC 2 cut(s) 434, 458
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BsuRI GGCC 2 cut(s) 874, 917
BtgZI GCGATG 1 cut(s) 83
BtsCI GGATG 4 cut(s) 148, 703, 709, 905
BtsIMutI CAGTG 5 cut(s) 101, 329, 344, 614, 691
Cac8I GCNNGC 4 cut(s) 456, 838, 854, 995
Cfr13I GGNCC 2 cut(s) 204, 873
Csp6I GTAC 1 cut(s) 1052
CviAII CATG 6 cut(s) 403, 492, 567, 842, 877, 899
CviQI GTAC 1 cut(s) 1052
DdeI CTNAG 5 cut(s) 138, 347, 360, 756, 806
DpnI GATC 4 cut(s) 343, 525, 594, 869
DpnII GATC 4 cut(s) 341, 523, 592, 867
EaeI YGGCCR 1 cut(s) 915
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Eco130I CCWWGG 1 cut(s) 508
Eco31I GGTCTC 1 cut(s) 84
Eco47I GGWCC 1 cut(s) 204
Eco57I CTGAAG 2 cut(s) 72, 125
EcoRII CCWGG 1 cut(s) 484
EcoT14I CCWWGG 1 cut(s) 508
ErhI CCWWGG 1 cut(s) 508
FaeI CATG 6 cut(s) 406, 495, 570, 845, 880, 902
FaqI GGGAC 4 cut(s) 41, 250, 391, 792
FatI CATG 6 cut(s) 402, 491, 566, 841, 876, 898
FauNDI CATATG 1 cut(s) 418
FbaI TGATCA 1 cut(s) 867
FblI GTMKAC 1 cut(s) 1000
Fnu4HI GCNGC 2 cut(s) 423, 447
FokI GGATG 4 cut(s) 155, 710, 716, 892
Fsp4HI GCNGC 2 cut(s) 423, 447
FspBI CTAG 7 cut(s) 191, 264, 368, 455, 837, 885, 1060
GluI GCNGC 2 cut(s) 423, 447
GsaI CCCAGC 1 cut(s) 412
HaeIII GGCC 2 cut(s) 874, 917
HapII CCGG 1 cut(s) 431
Hin1II CATG 6 cut(s) 406, 495, 570, 845, 880, 902
HinfI GANTC 3 cut(s) 20, 309, 662
HpaII CCGG 1 cut(s) 431
HphI GGTGA 1 cut(s) 752
Hpy166II GTNNAC 6 cut(s) 101, 207, 400, 497, 572, 1001
Hpy188I TCNGA 1 cut(s) 356
Hpy188III TCNNGA 4 cut(s) 50, 463, 543, 695
Hpy8I GTNNAC 6 cut(s) 101, 207, 400, 497, 572, 1001
HpyAV CCTTC 2 cut(s) 8, 961
HpyCH4III ACNGT 5 cut(s) 319, 397, 473, 609, 627
HpyCH4IV ACGT 2 cut(s) 45, 793
HpyCH4V TGCA 5 cut(s) 422, 446, 572, 707, 819
HpyF10VI GCNNNNNNNGC 5 cut(s) 431, 455, 816, 833, 990
HpyF3I CTNAG 5 cut(s) 138, 347, 360, 756, 806
HpySE526I ACGT 2 cut(s) 45, 793
Hsp92II CATG 6 cut(s) 406, 495, 570, 845, 880, 902
Ksp22I TGATCA 1 cut(s) 867
Kzo9I GATC 4 cut(s) 341, 523, 592, 867
LmnI GCTCC 5 cut(s) 4, 181, 356, 545, 613
Lsp1109I GCAGC 2 cut(s) 434, 458
LweI GCATC 3 cut(s) 694, 914, 1003
MaeI CTAG 7 cut(s) 191, 264, 368, 455, 837, 885, 1060
MaeII ACGT 2 cut(s) 45, 793
MaeIII GTNAC 3 cut(s) 186, 310, 473
MalI GATC 4 cut(s) 343, 525, 594, 869
MbiI CCGCTC 1 cut(s) 184
MboI GATC 4 cut(s) 341, 523, 592, 867
MboII GAAGA 5 cut(s) 215, 533, 593, 781, 1048
MfeI CAATTG 1 cut(s) 158
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 2 cut(s) 574, 805
MlsI TGGCCA 1 cut(s) 917
MluCI AATT 5 cut(s) 60, 112, 158, 710, 894
MluI ACGCGT 1 cut(s) 995
MluNI TGGCCA 1 cut(s) 917
MlyI GAGTC 2 cut(s) 318, 656
MmeI TCCRAC 2 cut(s) 334, 620
MnlI CCTC 7 cut(s) 51, 133, 342, 694, 815, 940, 1022
Mox20I TGGCCA 1 cut(s) 917
MscI TGGCCA 1 cut(s) 917
MseI TTAA 3 cut(s) 195, 213, 926
Msp20I TGGCCA 1 cut(s) 917
MspA1I CMGCKG 1 cut(s) 856
MspI CCGG 1 cut(s) 431
MspR9I CCNGG 2 cut(s) 431, 486
MunI CAATTG 1 cut(s) 158
MvaI CCWGG 1 cut(s) 486
MvnI CGCG 2 cut(s) 68, 997
MwoI GCNNNNNNNGC 5 cut(s) 431, 455, 816, 833, 990
NciI CCSGG 1 cut(s) 431
NdeI CATATG 1 cut(s) 418
NdeII GATC 4 cut(s) 341, 523, 592, 867
NheI GCTAGC 2 cut(s) 454, 836
NlaIII CATG 6 cut(s) 406, 495, 570, 845, 880, 902
NlaIV GGNNCC 2 cut(s) 6, 802
NmuCI GTSAC 1 cut(s) 310
NspI RCATGY 3 cut(s) 406, 495, 570
NspV TTCGAA 1 cut(s) 767
PciI ACATGT 2 cut(s) 402, 491
PfeI GAWTC 1 cut(s) 20
PflMI CCANNNNNTGG 1 cut(s) 899
PkrI GCNGC 2 cut(s) 424, 448
PleI GAGTC 2 cut(s) 317, 656
PpsI GAGTC 2 cut(s) 317, 656
PscI ACATGT 2 cut(s) 402, 491
PshAI GACNNNNGTC 1 cut(s) 96
Psp6I CCWGG 1 cut(s) 484
PspFI CCCAGC 1 cut(s) 408
PspGI CCWGG 1 cut(s) 484
PspN4I GGNNCC 2 cut(s) 6, 802
PspPI GGNCC 2 cut(s) 204, 873
PstI CTGCAG 1 cut(s) 448
PsuI RGATCY 1 cut(s) 523
PvuII CAGCTG 1 cut(s) 856
RsaI GTAC 1 cut(s) 1053
RsaNI GTAC 1 cut(s) 1052
SaqAI TTAA 3 cut(s) 195, 213, 926
SatI GCNGC 2 cut(s) 423, 447
Sau3AI GATC 4 cut(s) 341, 523, 592, 867
Sau96I GGNCC 2 cut(s) 204, 873
SchI GAGTC 2 cut(s) 318, 656
ScrFI CCNGG 2 cut(s) 431, 486
SduI GDGCHC 2 cut(s) 574, 805
SfaNI GCATC 3 cut(s) 694, 914, 1003
SfcI CTRYAG 3 cut(s) 130, 444, 582
SfuI TTCGAA 1 cut(s) 767
SinI GGWCC 1 cut(s) 204
SpeI ACTAGT 1 cut(s) 190
Sse9I AATT 5 cut(s) 60, 112, 158, 710, 894
SsiI CCGC 2 cut(s) 184, 982
SspMI CTAG 7 cut(s) 191, 264, 368, 455, 837, 885, 1060
StyD4I CCNGG 2 cut(s) 429, 484
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 5 cut(s) 319, 397, 473, 609, 627
TaiI ACGT 2 cut(s) 48, 796
TaqI TCGA 1 cut(s) 767
TasI AATT 5 cut(s) 60, 112, 158, 710, 894
TatI WGTACW 1 cut(s) 1051
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 3 cut(s) 195, 213, 926
Tru9I TTAA 3 cut(s) 195, 213, 926
TscAI CASTG 5 cut(s) 108, 329, 351, 614, 691
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 2 cut(s) 422, 446
Tsp45I GTSAC 1 cut(s) 310
TspDTI ATGAA 1 cut(s) 887
TspRI CASTG 5 cut(s) 108, 329, 351, 614, 691
Van91I CCANNNNNTGG 1 cut(s) 899
VneI GTGCAC 1 cut(s) 570
VpaK11BI GGWCC 1 cut(s) 204
XceI RCATGY 3 cut(s) 406, 495, 570
XcmI CCANNNNNNNNNTGG 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 1000
XspI CTAG 7 cut(s) 191, 264, 368, 455, 837, 885, 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.