pycom08g09850

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013078
Physical Location & Seq
Reverse (-)
8174903 .. 8177119
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g09850.1

Sequence Viewer

Length: 648 bp
ATGGTGTTCTACTTCAGAGCTCGATCGGACGTCGGCGATTTCACCATTTTCATGGGCCTCGACAAGTTCGAGAACGAGGAGCTCATCAAATATGGCTTCCCCGAAGACATTTGGTTCCACGTGGATAAAATGTCATCTGCCCATGTTTATGTTAGACTGCACAAAGGACAGACCATTGACGATATAAGCGAAGGCTTACTGGAGGATTGTGCTCAACTTGTCAAAGCAAATTCCATCCAAGGCAATAAGGTGAACAATGTTGATGTTGTTTACACTCCCTGGTCCAATTTGAAGAAAACTGCCTCCATGGATGTTGGTCAAGTTGGTTTCTACAATTCAAAGATGGTTCGAACAGTGAGAGTGGAGAAGCGGATCAATGAGGTAGTTAATAGATTGAACAGGACAAAAGTGGAAAGAAAGCCTGATTTGAAAGCTGAGCGAGAAGCAGTTAATGCAGCAGAAAGAGCTGAGAGAAAGCAGTTCCTGAGGGACAAGAAACGACGAGAGGAGATGGAAAGGCTTGAGAAGGAGAGACAGTTGGAACTAAGGAGCTACAAAGGTTTGATGAATTCTGAAAATATGACATCTAACAAACAAATTGCATCAACAAACAAGTCATTGCAGGAACTGGAAGAGGACTTCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF814

Protein Analysis

216

Amino Acids

25.24

Weight (kDa)

8.5

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NFACT-R_1 PF05670 1 - 112 4.1e-36 NFACT protein RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013078)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11500 AT5G11500
fragaria_vesca FvH4_2g36060
malus_domestica MD08G1117900.v1.1 MD15G1097400.v1.1
prunus_persica Prupe.1G452200_v2.0.a1 Prupe.1G452200_v2.0.a1
pyrus_communis pycom08g09850 pycom15g08930
rosa_chinensis RchiOBHm_Chr6g0305791
rosa_laevigata RLG00000010858
rosa_roxburghii Rroxscaffold_7G00162330
rosa_rugosa Rorug06G0344000
rosa_samantha Rh6AG455600 Rh6BG445500 Rh6CG469600 Rh6DG456300
rosa_wichuraiana Rw6G039750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 33
AciI CCGC 1 cut(s) 370
AclWI GGATC 1 cut(s) 380
AcsI RAATTY 2 cut(s) 229, 568
AcvI CACGTG 1 cut(s) 121
AcyI GRCGYC 1 cut(s) 30
AgsI TTSAA 4 cut(s) 292, 339, 397, 430
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 5 cut(s) 20, 82, 434, 467, 552
AluI AGCT 5 cut(s) 20, 82, 434, 467, 552
Alw21I GWGCWC 3 cut(s) 22, 84, 214
Alw26I GTCTC 1 cut(s) 526
AlwI GGATC 1 cut(s) 380
AlwNI CAGNNNCTG 2 cut(s) 484, 628
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 455
ApoI RAATTY 2 cut(s) 229, 568
AspS9I GGNCC 2 cut(s) 55, 282
AsuHPI GGTGA 2 cut(s) 34, 262
AsuII TTCGAA 1 cut(s) 349
AvaII GGWCC 1 cut(s) 282
AxyI CCTNAGG 1 cut(s) 485
BanII GRGCYC 2 cut(s) 22, 84
BbrPI CACGTG 1 cut(s) 121
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 3 cut(s) 22, 84, 214
BbvI GCAGC 1 cut(s) 467
BccI CCATC 3 cut(s) 242, 337, 505
BciT130I CCWGG 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 526
BisI GCNGC 1 cut(s) 456
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 1 cut(s) 457
Bme1390I CCNGG 1 cut(s) 280
Bme18I GGWCC 1 cut(s) 282
BmgT120I GGNCC 2 cut(s) 55, 282
BmiI GGNNCC 1 cut(s) 116
BmrFI CCNGG 1 cut(s) 280
BmsI GCATC 1 cut(s) 611
BpiI GAAGAC 1 cut(s) 111
BpmI CTGGAG 1 cut(s) 221
Bpu1102I GCTNAGC 1 cut(s) 435
Bpu14I TTCGAA 1 cut(s) 349
BpuEI CTTGAG 1 cut(s) 542
BsaAI YACGTR 1 cut(s) 121
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 3 cut(s) 238, 278, 306
BsaXI ACNNNNNCTCC 2 cut(s) 521, 551
Bse1I ACTGG 2 cut(s) 204, 633
Bse21I CCTNAGG 1 cut(s) 485
Bse3DI GCAATG 1 cut(s) 617
BseBI CCWGG 1 cut(s) 280
BseDI CCNNGG 3 cut(s) 238, 278, 306
BseGI GGATG 2 cut(s) 234, 316
BseMI GCAATG 1 cut(s) 617
BseMII CTCAG 3 cut(s) 426, 459, 476
BseNI ACTGG 2 cut(s) 204, 633
BseRI GAGGAG 2 cut(s) 92, 521
BseXI GCAGC 1 cut(s) 467
BsgI GTGCAG 1 cut(s) 143
Bsh1285I CGRYCG 1 cut(s) 26
BshFI GGCC 1 cut(s) 57
BsiEI CGRYCG 1 cut(s) 26
BsiHKAI GWGCWC 3 cut(s) 22, 84, 214
BslFI GGGAC 1 cut(s) 503
BsmAI GTCTC 1 cut(s) 526
BsmFI GGGAC 1 cut(s) 503
BsnI GGCC 1 cut(s) 57
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 3 cut(s) 22, 84, 214
Bsp143I GATC 2 cut(s) 23, 372
Bsp1720I GCTNAGC 1 cut(s) 435
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 1 cut(s) 370
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 3 cut(s) 427, 460, 477
BspLI GGNNCC 1 cut(s) 116
BspPI GGATC 1 cut(s) 380
BspT104I TTCGAA 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 617
BsrI ACTGG 2 cut(s) 204, 633
BssECI CCNNGG 3 cut(s) 238, 278, 306
BssMI GATC 2 cut(s) 23, 372
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 2 cut(s) 238, 306
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 2 cut(s) 355, 537
Bst6I CTCTTC 1 cut(s) 627
BstACI GRCGYC 1 cut(s) 30
BstAPI GCANNNNNTGC 1 cut(s) 452
BstBAI YACGTR 1 cut(s) 121
BstBI TTCGAA 1 cut(s) 349
BstDEI CTNAG 4 cut(s) 435, 468, 485, 545
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 2 cut(s) 234, 316
BstKTI GATC 2 cut(s) 26, 375
BstMAI GTCTC 1 cut(s) 526
BstMBI GATC 2 cut(s) 23, 372
BstMCI CGRYCG 1 cut(s) 26
BstMWI GCNNNNNNNGC 2 cut(s) 452, 464
BstNI CCWGG 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 467
BstV2I GAAGAC 1 cut(s) 111
BstXI CCANNNNNNTGG 1 cut(s) 52
Bsu36I CCTNAGG 1 cut(s) 485
BsuRI GGCC 1 cut(s) 57
BtgI CCRYGG 1 cut(s) 306
BtsCI GGATG 2 cut(s) 234, 316
BtsIMutI CAGTG 1 cut(s) 360
CaiI CAGNNNCTG 2 cut(s) 484, 628
Cfr13I GGNCC 2 cut(s) 55, 282
CviAII CATG 4 cut(s) 52, 143, 307, 643
DdeI CTNAG 4 cut(s) 435, 468, 485, 545
DpnI GATC 2 cut(s) 25, 374
DpnII GATC 2 cut(s) 23, 372
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
Ecl136II GAGCTC 2 cut(s) 20, 82
Eco130I CCWWGG 2 cut(s) 238, 306
Eco24I GRGCYC 2 cut(s) 22, 84
Eco47I GGWCC 1 cut(s) 282
Eco53kI GAGCTC 2 cut(s) 20, 82
Eco72I CACGTG 1 cut(s) 121
Eco81I CCTNAGG 1 cut(s) 485
EcoICRI GAGCTC 2 cut(s) 20, 82
EcoRI GAATTC 1 cut(s) 568
EcoRII CCWGG 1 cut(s) 278
EcoT14I CCWWGG 2 cut(s) 238, 306
EcoT38I GRGCYC 2 cut(s) 22, 84
ErhI CCWWGG 2 cut(s) 238, 306
FaeI CATG 4 cut(s) 55, 146, 310, 646
FaiI YATR 8 cut(s) 53, 93, 144, 150, 185, 308, 581, 644
FaqI GGGAC 1 cut(s) 503
FatI CATG 4 cut(s) 51, 142, 306, 642
Fnu4HI GCNGC 1 cut(s) 456
FokI GGATG 2 cut(s) 221, 323
FriOI GRGCYC 2 cut(s) 22, 84
Fsp4HI GCNGC 1 cut(s) 456
GluI GCNGC 1 cut(s) 456
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 1 cut(s) 57
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 4 cut(s) 55, 146, 310, 646
HphI GGTGA 2 cut(s) 34, 262
Hpy166II GTNNAC 2 cut(s) 253, 271
Hpy188I TCNGA 3 cut(s) 17, 28, 574
Hpy188III TCNNGA 2 cut(s) 70, 484
Hpy8I GTNNAC 2 cut(s) 253, 271
Hpy99I CGWCG 2 cut(s) 35, 504
HpyAV CCTTC 2 cut(s) 185, 520
HpyCH4III ACNGT 2 cut(s) 355, 537
HpyCH4IV ACGT 2 cut(s) 30, 120
HpyCH4V TGCA 4 cut(s) 160, 455, 602, 622
HpyF10VI GCNNNNNNNGC 2 cut(s) 452, 464
HpyF3I CTNAG 4 cut(s) 435, 468, 485, 545
HpySE526I ACGT 2 cut(s) 30, 120
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 4 cut(s) 55, 146, 310, 646
Kzo9I GATC 2 cut(s) 23, 372
LmnI GCTCC 2 cut(s) 79, 549
LpnPI CCDG 8 cut(s) 185, 265, 292, 385, 435, 497, 608, 614
Lsp1109I GCAGC 1 cut(s) 467
LweI GCATC 1 cut(s) 611
MaeII ACGT 2 cut(s) 30, 120
MalI GATC 2 cut(s) 25, 374
MboI GATC 2 cut(s) 23, 372
MboII GAAGA 3 cut(s) 116, 304, 644
MhlI GDGCHC 3 cut(s) 22, 84, 214
MluCI AATT 5 cut(s) 229, 286, 334, 568, 597
MmeI TCCRAC 1 cut(s) 519
MnlI CCTC 8 cut(s) 68, 70, 196, 313, 373, 480, 499, 628
MseI TTAA 2 cut(s) 387, 450
MslI CAYNNNNRTG 2 cut(s) 50, 147
MspR9I CCNGG 1 cut(s) 280
MvaI CCWGG 1 cut(s) 280
MwoI GCNNNNNNNGC 2 cut(s) 452, 464
NcoI CCATGG 1 cut(s) 306
NdeII GATC 2 cut(s) 23, 372
NlaIII CATG 4 cut(s) 55, 146, 310, 646
NlaIV GGNNCC 1 cut(s) 116
NspV TTCGAA 1 cut(s) 349
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 186
PkrI GCNGC 1 cut(s) 457
Ple19I CGATCG 1 cut(s) 26
PmaCI CACGTG 1 cut(s) 121
PmlI CACGTG 1 cut(s) 121
Ppu21I YACGTR 1 cut(s) 121
Psp124BI GAGCTC 2 cut(s) 22, 84
Psp6I CCWGG 1 cut(s) 278
PspCI CACGTG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 278
PspN4I GGNNCC 1 cut(s) 116
PspPI GGNCC 2 cut(s) 55, 282
PstNI CAGNNNCTG 2 cut(s) 484, 628
PvuI CGATCG 1 cut(s) 26
RseI CAYNNNNRTG 2 cut(s) 50, 147
SacI GAGCTC 2 cut(s) 22, 84
SaqAI TTAA 2 cut(s) 387, 450
SatI GCNGC 1 cut(s) 456
Sau3AI GATC 2 cut(s) 23, 372
Sau96I GGNCC 2 cut(s) 55, 282
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 3 cut(s) 22, 84, 214
SfaNI GCATC 1 cut(s) 611
SfuI TTCGAA 1 cut(s) 349
SinI GGWCC 1 cut(s) 282
SmiMI CAYNNNNRTG 2 cut(s) 50, 147
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 5 cut(s) 229, 286, 334, 568, 597
SsiI CCGC 1 cut(s) 370
SstI GAGCTC 2 cut(s) 22, 84
StyD4I CCNGG 1 cut(s) 278
StyI CCWWGG 2 cut(s) 238, 306
TaaI ACNGT 2 cut(s) 355, 537
TaiI ACGT 2 cut(s) 33, 123
TaqI TCGA 4 cut(s) 22, 60, 69, 349
TasI AATT 5 cut(s) 229, 286, 334, 568, 597
Tru1I TTAA 2 cut(s) 387, 450
Tru9I TTAA 2 cut(s) 387, 450
TscAI CASTG 1 cut(s) 360
TseI GCWGC 1 cut(s) 455
TspDTI ATGAA 3 cut(s) 40, 581, 631
TspRI CASTG 1 cut(s) 360
VpaK11BI GGWCC 1 cut(s) 282
XapI RAATTY 2 cut(s) 229, 568
ZraI GACGTC 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.