pycom08g10940

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0006441
Physical Location & Seq
Reverse (-)
9179620 .. 9185110
5491 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2037 bp
ATGACCGTCACGGATTCCCACCACTTCCACGCGCCGCATTGCGCGAAAGGGTGGCAAGTCAAAAACGCTTACATTTGGGCCCCAACTCTGAGCTCCTCCGAGCTCCCCCGCTACTCCCCCACCATGGCCGGCGATAAGGCCATCAGCCTCGAAGAGATAAAAAACGAGTCCGTCGATCTGGAACGGATTCCGGTAGAGGAAGTCTTTGAGCAGCTGAAATGTACAAGAGAAGGTCTGACTGGAGAAGAAGGAGCCAACAGGCTTCAAGTCTTTGGACCAAACAAACTAGAAGAGAAAAAGGAGAGCAAACTGCTCAAGTTCTTGGGATTTATGTGGAATCCTTTGTCATGGGTCATGGAAGCTGCTGCTCTGATGGCTATTGTCTTGGCAAATGGTGATGGAAGGCCTCCTGATTGGCAGGACTTTGTTGGCATTGTTGTTCTTCTGGTCATTAACTCTACTATCAGTTTCATCGAAGAAAACAACGCTGGTAATGCTGCTGCAGCCCTTATGGCCGGTCTAGCTCCCAAGACTAAGGTTCTTAGAGATGGTCGATGGACTGAGCAAGATGCTTCAATTTTGGTGCCTGGAGACATAATCAGTATCAAATTGGGAGATATTGTTCCTGCTGATGCCCGCCTTCTCGAGGGTGATCCTCTGAAAATCGATCAATCTGCCCTTACTGGCGAGTCCCTTCCAGTTACTAAGAATCCTTCGGATGAAGTGTTTTCTGGCTCAACATGTAAACAGGGAGAAATTGAAGCAGTTGTTATTGCTACTGGTGTGCACACCTTCTTTGGCAAGGCTGCACATCTGGTGGACAACACCAATCAAGTTGGGCACTTCCAGAAAGTTCTCACTGCCATCGGGAACTTCTGCATTTGTTCAATTGCTGTGGGGATACTAATTGAGATAATAGTAATGTACCCAATACAGAAGCGCAAGTACAGGCAAGGGATTGATAATTTGCTGGTTCTCTTGATTGGAGGAATCCCAATTGCCATGCCAACTGTTTTATCAGTCACCATGGCTATTGGCTCCCACAGGCTTGCTCAGCAGGGAGCTATCACCAAGAGAATGACTGCCATTGAGGAAATGGCTGGCATGGACGTCCTCTGCAGTGACAAGACTGGGACTTTGACCCTGAACAAGCTCTCAGTTGATAGAAATCTGATTGAAGTGTTTGCCAAGGGTGTGGAGAAAGAGCATGTTATGCTTCTTGCAGCAAGGGCTTCTAGGACTGAAAATCAGGATGCTATTGATGCTGCAATTGTAGGAATGCTTGCTGATCCTAAGGAGGCACGAGCTGGTATAAGAGAAGTCCATTTCCTACCGTTCAACCCCGTAGACAAGAGGACTGCTCTGACCTACATCGATTCCGATGGAAATTGGCATCGCGCTAGTAAAGGTGCCCCTGAGCAGATATTAACCCTATGCAACTGCAAGGAAGATTTCAAGAAAAAGGTGCATGGGGTGATTGATAAGTTTGCTGAACGTGGACTTCGTTCTCTGGGTGTTGCAAGACAGCAAGTACCAGAAAAAACAAAAGAGAGTCCAGGGACACCATGGCAGTTCGTTGGTTTATTGCCGTTATTTGATCCTCCCAGGCATGACAGTGCAGAAACAATTCGCAGGGCACTCAATCTCGGTGTGAATGTGAAGATGATTACAGGGGATCAGCTTGCCATTGGCAAGGAAACTGGAAGGAGGCTTGGTATGGGAACAAACATGTACCCATCCTCCGCATTGCTTGGCCAAGACAAGGATGCAGCCATAGCTTCCCTCCCCGTGGACGAGTTGATTGAGAAGGCTGATGGATTTGCTGGAGTATTTCCTGAACACAAATATGAAATTGTAAAGAGGCTGCAAGAGAGGAAGCACATCTGTGGAATGACTGGAGATGGTGTCAATGACGCTCCTGCTTTGAAAAAAGCAGATATTGGTATTGCCGTAGCTGATGCTACTGATGCTGCAAGAGGTGCTTCGGATATTGTTCTCACTGAACCAGGGCTAAGTGTTTCAAGAGATCGATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

679

Amino Acids

73.55

Weight (kDa)

5.92

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 62 - 125 3.6e-15 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 175 - 273 8.5e-31 P-type ATPase actuator domain
Hydrolase PF00702 369 - 646 2.6e-14 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 417 - 537 5.2e-06 P-type ATPase, cytoplasmic domain N
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1113
AccB1I GGYRCC 2 cut(s) 583, 1409
AccI GTMKAC 1 cut(s) 1347
AccII CGCG 3 cut(s) 32, 44, 1398
AciI CCGC 4 cut(s) 35, 109, 637, 1743
AclWI GGATC 4 cut(s) 647, 1283, 1592, 1683
AcoI YGGCCR 3 cut(s) 126, 513, 1753
AcyI GRCGYC 1 cut(s) 1110
AfaI GTAC 5 cut(s) 223, 926, 947, 1533, 1733
AfiI CCNNNNNNNGG 4 cut(s) 124, 646, 1762, 1789
AflIII ACRYGT 2 cut(s) 740, 1728
AgsI TTSAA 9 cut(s) 266, 576, 761, 888, 1178, 1339, 1456, 1927, 2022
AjnI CCWGG 4 cut(s) 586, 1555, 1604, 2005
AleI CACNNNNGTG 1 cut(s) 1884
AloI GAACNNNNNNTCC 2 cut(s) 606, 638
Alw21I GWGCWC 3 cut(s) 95, 105, 789
Alw26I GTCTC 1 cut(s) 585
Alw44I GTGCAC 1 cut(s) 785
AlwI GGATC 4 cut(s) 647, 1283, 1592, 1683
Ama87I CYCGRG 1 cut(s) 644
AoxI GGCC 6 cut(s) 78, 126, 138, 404, 513, 1753
ApaI GGGCCC 1 cut(s) 82
ApaLI GTGCAC 1 cut(s) 785
ArsI GACNNNNNNTTYG 2 cut(s) 1120, 1152
Asp700I GAANNNNTTC 2 cut(s) 186, 1626
AspLEI GCGC 4 cut(s) 34, 44, 942, 1400
AspS9I GGNCC 3 cut(s) 78, 79, 275
AsuHPI GGTGA 5 cut(s) 407, 662, 1015, 1060, 1486
AvaI CYCGRG 1 cut(s) 644
AvaII GGWCC 1 cut(s) 275
AxyI CCTNAGG 1 cut(s) 1293
BaeGI GKGCMC 5 cut(s) 82, 789, 843, 1414, 1639
BalI TGGCCA 1 cut(s) 1755
BanI GGYRCC 2 cut(s) 583, 1409
BanII GRGCYC 3 cut(s) 82, 95, 105
BarI GAAGNNNNNNTAC 2 cut(s) 929, 961
BauI CACGAG 1 cut(s) 1302
Bbv12I GWGCWC 3 cut(s) 95, 105, 789
BceAI ACGGC 2 cut(s) 1573, 1934
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BciT130I CCWGG 4 cut(s) 588, 1557, 1606, 2007
BciVI GTATCC 1 cut(s) 894
BcoDI GTCTC 1 cut(s) 585
BfaI CTAG 4 cut(s) 287, 521, 1236, 1401
BfmI CTRYAG 2 cut(s) 501, 1117
BfuI GTATCC 1 cut(s) 894
BglI GCCNNNNNGGC 1 cut(s) 512
BlpI GCTNAGC 1 cut(s) 1053
Bme1390I CCNGG 4 cut(s) 588, 1557, 1606, 2007
Bme18I GGWCC 1 cut(s) 275
BmeT110I CYCGRG 1 cut(s) 644
BmgT120I GGNCC 3 cut(s) 78, 79, 275
BmiI GGNNCC 6 cut(s) 80, 81, 253, 585, 1039, 1411
BmrFI CCNGG 4 cut(s) 588, 1557, 1606, 2007
BmrI ACTGGG 1 cut(s) 1140
BmsI GCATC 8 cut(s) 559, 622, 1243, 1252, 1402, 1756, 1948, 1957
BmuI ACTGGG 1 cut(s) 1140
BplI GAGNNNNNCTC 2 cut(s) 1345, 1377
BpmI CTGGAG 4 cut(s) 261, 609, 1845, 1917
Bpu10I CCTNAGC 1 cut(s) 1416
Bpu1102I GCTNAGC 1 cut(s) 1053
BpuEI CTTGAG 1 cut(s) 299
Bsa29I ATCGAT 3 cut(s) 666, 1374, 2029
BsaBI GATNNNNATC 1 cut(s) 1167
BsaHI GRCGYC 1 cut(s) 1110
BsaJI CCNNGG 8 cut(s) 123, 1026, 1188, 1556, 1565, 1604, 1788, 2006
BsaWI WCCGGW 1 cut(s) 190
BsaXI ACNNNNNCTCC 8 cut(s) 606, 636, 1699, 1724, 1729, 1754, 1890, 1920
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 646, 1762, 1789
Bse118I RCCGGY 2 cut(s) 128, 515
Bse1I ACTGG 7 cut(s) 244, 688, 698, 784, 1135, 1705, 1900
Bse21I CCTNAGG 1 cut(s) 1293
Bse3DI GCAATG 2 cut(s) 37, 1745
Bse8I GATNNNNATC 1 cut(s) 1167
BseBI CCWGG 4 cut(s) 588, 1557, 1606, 2007
BseCI ATCGAT 3 cut(s) 666, 1374, 2029
BseDI CCNNGG 8 cut(s) 123, 1026, 1188, 1556, 1565, 1604, 1788, 2006
BseGI GGATG 4 cut(s) 724, 1258, 1736, 1771
BseJI GATNNNNATC 1 cut(s) 1167
BseLI CCNNNNNNNGG 4 cut(s) 124, 646, 1762, 1789
BseMI GCAATG 2 cut(s) 37, 1745
BseMII CTCAG 5 cut(s) 80, 552, 1067, 1170, 1407
BseNI ACTGG 7 cut(s) 244, 688, 698, 784, 1135, 1705, 1900
BseRI GAGGAG 1 cut(s) 85
BseSI GKGCMC 5 cut(s) 82, 789, 843, 1414, 1639
BsgI GTGCAG 2 cut(s) 792, 1638
Bsh1236I CGCG 3 cut(s) 32, 44, 1398
BshFI GGCC 6 cut(s) 80, 128, 140, 406, 515, 1755
BshNI GGYRCC 2 cut(s) 583, 1409
BshVI ATCGAT 3 cut(s) 666, 1374, 2029
BsiHKAI GWGCWC 3 cut(s) 95, 105, 789
BsiHKCI CYCGRG 1 cut(s) 644
BsiSI CCGG 3 cut(s) 129, 191, 516
BslFI GGGAC 3 cut(s) 676, 1147, 1573
BslI CCNNNNNNNGG 4 cut(s) 124, 646, 1762, 1789
BsmAI GTCTC 1 cut(s) 585
BsmFI GGGAC 3 cut(s) 676, 1147, 1573
BsmI GAATGC 1 cut(s) 1284
BsnI GGCC 6 cut(s) 80, 128, 140, 406, 515, 1755
BsoBI CYCGRG 1 cut(s) 644
Bsp120I GGGCCC 1 cut(s) 78
Bsp1286I GDGCHC 7 cut(s) 82, 95, 105, 789, 843, 1414, 1639
Bsp1407I TGTACA 1 cut(s) 221
Bsp143I GATC 8 cut(s) 175, 652, 667, 1288, 1597, 1675, 2026, 2030
Bsp1720I GCTNAGC 1 cut(s) 1053
Bsp19I CCATGG 3 cut(s) 123, 1026, 1565
BspACI CCGC 4 cut(s) 35, 109, 637, 1743
BspANI GGCC 6 cut(s) 80, 128, 140, 406, 515, 1755
BspCNI CTCAG 5 cut(s) 81, 553, 1066, 1169, 1408
BspDI ATCGAT 3 cut(s) 666, 1374, 2029
BspFNI CGCG 3 cut(s) 32, 44, 1398
BspLI GGNNCC 6 cut(s) 80, 81, 253, 585, 1039, 1411
BspMAI CTGCAG 2 cut(s) 505, 1121
BspPI GGATC 4 cut(s) 647, 1283, 1592, 1683
BspT107I GGYRCC 2 cut(s) 583, 1409
BsrDI GCAATG 2 cut(s) 37, 1745
BsrFI RCCGGY 2 cut(s) 128, 515
BsrGI TGTACA 1 cut(s) 221
BsrI ACTGG 7 cut(s) 244, 688, 698, 784, 1135, 1705, 1900
BssAI RCCGGY 2 cut(s) 128, 515
BssECI CCNNGG 8 cut(s) 123, 1026, 1188, 1556, 1565, 1604, 1788, 2006
BssMI GATC 8 cut(s) 175, 652, 667, 1288, 1597, 1675, 2026, 2030
BssNI GRCGYC 1 cut(s) 1110
BssSI CACGAG 1 cut(s) 1302
BssT1I CCWWGG 4 cut(s) 123, 1026, 1188, 1565
Bst2BI CACGAG 1 cut(s) 1302
Bst2UI CCWGG 4 cut(s) 588, 1557, 1606, 2007
Bst4CI ACNGT 4 cut(s) 7, 1012, 1335, 1616
Bst6I CTCTTC 2 cut(s) 147, 285
BstACI GRCGYC 1 cut(s) 1110
BstAPI GCANNNNNTGC 2 cut(s) 1213, 1979
BstAUI TGTACA 1 cut(s) 221
BstC8I GCNNGC 6 cut(s) 130, 637, 1050, 1102, 1284, 1683
BstDSI CCRYGG 4 cut(s) 123, 1026, 1565, 1788
BstENI CCTNNNNNAGG 1 cut(s) 644
BstF5I GGATG 4 cut(s) 724, 1258, 1736, 1771
BstFNI CGCG 3 cut(s) 32, 44, 1398
BstHHI GCGC 4 cut(s) 34, 44, 942, 1400
BstKTI GATC 8 cut(s) 178, 655, 670, 1291, 1600, 1678, 2029, 2033
BstMAI GTCTC 1 cut(s) 585
BstMBI GATC 8 cut(s) 175, 652, 667, 1288, 1597, 1675, 2026, 2030
BstNI CCWGG 4 cut(s) 588, 1557, 1606, 2007
BstNSI RCATGY 3 cut(s) 744, 1211, 1732
BstSCI CCNGG 4 cut(s) 586, 1555, 1604, 2005
BstSFI CTRYAG 2 cut(s) 501, 1117
BstSLI GKGCMC 5 cut(s) 82, 789, 843, 1414, 1639
BstUI CGCG 3 cut(s) 32, 44, 1398
BstXI CCANNNNNNTGG 1 cut(s) 1195
Bsu15I ATCGAT 3 cut(s) 666, 1374, 2029
Bsu36I CCTNAGG 1 cut(s) 1293
BsuI GTATCC 1 cut(s) 894
BsuRI GGCC 6 cut(s) 80, 128, 140, 406, 515, 1755
BsuTUI ATCGAT 3 cut(s) 666, 1374, 2029
BtgI CCRYGG 4 cut(s) 123, 1026, 1565, 1788
BtgZI GCGATG 1 cut(s) 1379
BtsCI GGATG 4 cut(s) 724, 1258, 1736, 1771
BtsI GCAGTG 2 cut(s) 858, 1126
BtsIMutI CAGTG 4 cut(s) 858, 1126, 1621, 1998
Cac8I GCNNGC 6 cut(s) 130, 637, 1050, 1102, 1284, 1683
CfoI GCGC 4 cut(s) 34, 44, 942, 1400
Cfr10I RCCGGY 2 cut(s) 128, 515
Cfr13I GGNCC 3 cut(s) 78, 79, 275
ClaI ATCGAT 3 cut(s) 666, 1374, 2029
CseI GACGC 1 cut(s) 1922
Csp6I GTAC 5 cut(s) 222, 925, 946, 1532, 1732
CspCI CAANNNNNGTGG 2 cut(s) 876, 911
CviQI GTAC 5 cut(s) 222, 925, 946, 1532, 1732
DpnI GATC 8 cut(s) 177, 654, 669, 1290, 1599, 1677, 2028, 2032
DpnII GATC 8 cut(s) 175, 652, 667, 1288, 1597, 1675, 2026, 2030
EaeI YGGCCR 3 cut(s) 126, 513, 1753
Eam1104I CTCTTC 2 cut(s) 147, 285
EarI CTCTTC 2 cut(s) 147, 285
Ecl136II GAGCTC 2 cut(s) 93, 103
Eco130I CCWWGG 4 cut(s) 123, 1026, 1188, 1565
Eco147I AGGCCT 1 cut(s) 406
Eco24I GRGCYC 3 cut(s) 82, 95, 105
Eco47I GGWCC 1 cut(s) 275
Eco53kI GAGCTC 2 cut(s) 93, 103
Eco81I CCTNAGG 1 cut(s) 1293
Eco88I CYCGRG 1 cut(s) 644
EcoICRI GAGCTC 2 cut(s) 93, 103
EcoNI CCTNNNNNAGG 1 cut(s) 644
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 4 cut(s) 586, 1555, 1604, 2005
EcoT14I CCWWGG 4 cut(s) 123, 1026, 1188, 1565
EcoT38I GRGCYC 3 cut(s) 82, 95, 105
ErhI CCWWGG 4 cut(s) 123, 1026, 1188, 1565
FalI AAGNNNNNCTT 2 cut(s) 1966, 1998
FaqI GGGAC 3 cut(s) 676, 1147, 1573
FauI CCCGC 2 cut(s) 116, 644
FblI GTMKAC 1 cut(s) 1347
FokI GGATG 4 cut(s) 731, 1265, 1723, 1778
FriOI GRGCYC 3 cut(s) 82, 95, 105
FspBI CTAG 4 cut(s) 287, 521, 1236, 1401
GlaI GCGC 4 cut(s) 33, 43, 941, 1399
GsuI CTGGAG 4 cut(s) 261, 609, 1845, 1917
HaeIII GGCC 6 cut(s) 80, 128, 140, 406, 515, 1755
HapII CCGG 3 cut(s) 129, 191, 516
HgaI GACGC 1 cut(s) 1922
HhaI GCGC 4 cut(s) 34, 44, 942, 1400
Hin1I GRCGYC 1 cut(s) 1110
Hin6I GCGC 4 cut(s) 32, 42, 940, 1398
HinP1I GCGC 4 cut(s) 32, 42, 940, 1398
HinfI GANTC 9 cut(s) 14, 167, 187, 337, 689, 709, 990, 1376, 1552
HpaII CCGG 3 cut(s) 129, 191, 516
HphI GGTGA 5 cut(s) 407, 662, 1015, 1060, 1486
Hpy166II GTNNAC 6 cut(s) 746, 787, 820, 1348, 1499, 1792
Hpy8I GTNNAC 6 cut(s) 746, 787, 820, 1348, 1499, 1792
Hpy99I CGWCG 1 cut(s) 176
HpyAV CCTTC 9 cut(s) 224, 242, 396, 650, 704, 723, 802, 1698, 1801
HpyCH4III ACNGT 4 cut(s) 7, 1012, 1335, 1616
HpyCH4IV ACGT 2 cut(s) 1110, 1495
HpySE526I ACGT 2 cut(s) 1110, 1495
Hsp92I GRCGYC 1 cut(s) 1110
HspAI GCGC 4 cut(s) 32, 42, 940, 1398
KroI GCCGGC 1 cut(s) 128
KroNI GCCGGC 1 cut(s) 130
Kzo9I GATC 8 cut(s) 175, 652, 667, 1288, 1597, 1675, 2026, 2030
LmnI GCTCC 7 cut(s) 98, 108, 251, 529, 1043, 1061, 1921
LweI GCATC 8 cut(s) 559, 622, 1243, 1252, 1402, 1756, 1948, 1957
MaeI CTAG 4 cut(s) 287, 521, 1236, 1401
MaeII ACGT 2 cut(s) 1110, 1495
MaeIII GTNAC 4 cut(s) 7, 700, 1021, 1121
MalI GATC 8 cut(s) 177, 654, 669, 1290, 1599, 1677, 2028, 2032
MboI GATC 8 cut(s) 175, 652, 667, 1288, 1597, 1675, 2026, 2030
MboII GAAGA 7 cut(s) 164, 257, 302, 434, 488, 1460, 1672
MfeI CAATTG 3 cut(s) 888, 996, 1269
MhlI GDGCHC 7 cut(s) 82, 95, 105, 789, 843, 1414, 1639
MlsI TGGCCA 1 cut(s) 1755
MluNI TGGCCA 1 cut(s) 1755
MlyI GAGTC 3 cut(s) 176, 698, 1561
Mox20I TGGCCA 1 cut(s) 1755
MroNI GCCGGC 1 cut(s) 128
MroXI GAANNNNTTC 2 cut(s) 186, 1626
MscI TGGCCA 1 cut(s) 1755
MseI TTAA 2 cut(s) 453, 1427
MslI CAYNNNNRTG 3 cut(s) 1614, 1845, 1884
Msp20I TGGCCA 1 cut(s) 1755
MspA1I CMGCKG 1 cut(s) 214
MspI CCGG 3 cut(s) 129, 191, 516
MspR9I CCNGG 4 cut(s) 588, 1557, 1606, 2007
MunI CAATTG 3 cut(s) 888, 996, 1269
Mva1269I GAATGC 1 cut(s) 1284
MvaI CCWGG 4 cut(s) 588, 1557, 1606, 2007
MvnI CGCG 3 cut(s) 32, 44, 1398
NaeI GCCGGC 1 cut(s) 130
NcoI CCATGG 3 cut(s) 123, 1026, 1565
NdeII GATC 8 cut(s) 175, 652, 667, 1288, 1597, 1675, 2026, 2030
NgoMIV GCCGGC 1 cut(s) 128
NlaIV GGNNCC 6 cut(s) 80, 81, 253, 585, 1039, 1411
NmuCI GTSAC 3 cut(s) 7, 1021, 1121
NspI RCATGY 3 cut(s) 744, 1211, 1732
OliI CACNNNNGTG 1 cut(s) 1884
PaeR7I CTCGAG 1 cut(s) 644
PceI AGGCCT 1 cut(s) 406
PciI ACATGT 2 cut(s) 740, 1728
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 1501
PctI GAATGC 1 cut(s) 1284
PdiI GCCGGC 1 cut(s) 130
PdmI GAANNNNTTC 2 cut(s) 186, 1626
PfeI GAWTC 6 cut(s) 14, 187, 337, 709, 990, 1376
PleI GAGTC 3 cut(s) 175, 697, 1560
PpsI GAGTC 3 cut(s) 175, 697, 1560
PscI ACATGT 2 cut(s) 740, 1728
Psp124BI GAGCTC 2 cut(s) 95, 105
Psp6I CCWGG 4 cut(s) 586, 1555, 1604, 2005
PspGI CCWGG 4 cut(s) 586, 1555, 1604, 2005
PspN4I GGNNCC 6 cut(s) 80, 81, 253, 585, 1039, 1411
PspOMI GGGCCC 1 cut(s) 78
PspPI GGNCC 3 cut(s) 78, 79, 275
PstI CTGCAG 2 cut(s) 505, 1121
PvuII CAGCTG 1 cut(s) 214
RsaI GTAC 5 cut(s) 223, 926, 947, 1533, 1733
RsaNI GTAC 5 cut(s) 222, 925, 946, 1532, 1732
RseI CAYNNNNRTG 3 cut(s) 1614, 1845, 1884
SacI GAGCTC 2 cut(s) 95, 105
SaqAI TTAA 2 cut(s) 453, 1427
Sau3AI GATC 8 cut(s) 175, 652, 667, 1288, 1597, 1675, 2026, 2030
Sau96I GGNCC 3 cut(s) 78, 79, 275
SchI GAGTC 3 cut(s) 176, 698, 1561
ScrFI CCNGG 4 cut(s) 588, 1557, 1606, 2007
SduI GDGCHC 7 cut(s) 82, 95, 105, 789, 843, 1414, 1639
SfaNI GCATC 8 cut(s) 559, 622, 1243, 1252, 1402, 1756, 1948, 1957
SfcI CTRYAG 2 cut(s) 501, 1117
Sfr274I CTCGAG 1 cut(s) 644
SinI GGWCC 1 cut(s) 275
SlaI CTCGAG 1 cut(s) 644
SmiMI CAYNNNNRTG 3 cut(s) 1614, 1845, 1884
SmlI CTYRAG 2 cut(s) 314, 644
SmoI CTYRAG 2 cut(s) 314, 644
SseBI AGGCCT 1 cut(s) 406
SsiI CCGC 4 cut(s) 35, 109, 637, 1743
SspMI CTAG 4 cut(s) 287, 521, 1236, 1401
SstI GAGCTC 2 cut(s) 95, 105
StuI AGGCCT 1 cut(s) 406
StyD4I CCNGG 4 cut(s) 586, 1555, 1604, 2005
StyI CCWWGG 4 cut(s) 123, 1026, 1188, 1565
TaaI ACNGT 4 cut(s) 7, 1012, 1335, 1616
TaiI ACGT 2 cut(s) 1113, 1498
TaqI TCGA 8 cut(s) 150, 174, 474, 553, 645, 666, 1374, 2029
TatI WGTACW 2 cut(s) 221, 945
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 6 cut(s) 14, 187, 337, 709, 990, 1376
Tru1I TTAA 2 cut(s) 453, 1427
Tru9I TTAA 2 cut(s) 453, 1427
TscAI CASTG 4 cut(s) 865, 1126, 1621, 2005
TseFI GTSAC 3 cut(s) 7, 1021, 1121
Tsp45I GTSAC 3 cut(s) 7, 1021, 1121
TspDTI ATGAA 3 cut(s) 460, 735, 1863
TspGWI ACGGA 3 cut(s) 26, 160, 199
TspRI CASTG 4 cut(s) 865, 1126, 1621, 2005
VneI GTGCAC 1 cut(s) 785
VpaK11BI GGWCC 1 cut(s) 275
XagI CCTNNNNNAGG 1 cut(s) 644
XceI RCATGY 3 cut(s) 744, 1211, 1732
XcmI CCANNNNNNNNNTGG 2 cut(s) 1093, 1563
XhoI CTCGAG 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 1347
XmnI GAANNNNTTC 2 cut(s) 186, 1626
XspI CTAG 4 cut(s) 287, 521, 1236, 1401
ZraI GACGTC 1 cut(s) 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.