pycom08g14060

protein At4g08330, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011319
Physical Location & Seq
Forward (+)
13099997 .. 13101751
1755 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 894 bp
ATGTCGTCAGCCCCTCTCACTGTGACCATAAACTACGAATGCCTTCTTGCCGTTAATGTCCACGTCAGCACAGAGAAATCTGTTCACAGGAAGAGAGCTCGCAGACGCCGCGGAGTCTATTTACTCTGCAATTCATTCATCTCGGCTTTCTTCTTTCCGGACACGGCCACTATCTGGTTCCTGATACGGCGTCGTATCCGGTCGAAATCCCAGCTCCAACTGCCACTGTTGGCGGTTAAACTCCCCCGAATCAGGCGGGATACGCTCTTCGCCATCCCACTTATCCACATCTGTCTTTCCTCTGGCCACAACTGTAATTTCAATACAGTCGAATTTTCAGAAAAGTCTCCGCCACCGTCACGGCCACCGTCTCAGCCGTTGTTTCCAGCGTCTCGGATGGAGAAATCGAATATTTTCAGAGGCAGTTACCTCAACGGAGCTTACCATCCTTCCTTCTCCTCTTCTTCTTCTCGCGCCGATGTCAATTACAGCTGTGGTTCTTGTGGGTATGAGCTAAACCTAAGTTCCAGCAACCGGAACACTTCTACGATTGGCTCTAATAAGTATGGAAAATCTATAAAGCGAGGGATCATATCATTCTTTAACATCGATGAGAGCAGATTTACTCAGGTTGATGAACTTAAATGTAAACCCCATTTCTCTAAGCGTTCATGGGGTTTGTTCAGCCGAAGAACTAAACTTCTTTGTCGCAAGTGTGGTAACCATATAGGAAATGCTTATGATGAGTACACTTCATCGTCTTACCCGCTTGTGTTAGATGAATCAGATTCATCCGCAGGTCATGAAGTTACCAAATGCAGAAAATTTGATGTTAGAATTCGCGCTTTGCAACCTTCAAATTCTGAAGAATTTGGCCCTTCAATTTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

298

Amino Acids

33.63

Weight (kDa)

9.81

Isoelectric Point (pI)

67.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g08330 PF24046 156 - 286 3.7e-54 At4g08330 beta-tent
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011319)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08330 AT4G08330
fragaria_vesca FvH4_2g26960 FvH4_2g26960
malus_domestica MD08G1162700.v1.1 MD15G1348300.v1.1
prunus_persica Prupe.1G497200_v2.0.a1 Prupe.1G497200_v2.0.a1
pyrus_communis pycom08g14060 pycom15g31200
rosa_chinensis RchiOBHm_Chr6g0295821
rosa_laevigata RLG00000011765
rosa_multiflora Rmu_ssc0000086.1_g000001
rosa_roxburghii Rroxscaffold_7G00172150
rosa_rugosa Rorug06G0254100
rosa_samantha Rh6AG368100 Rh6BG375700 Rh6CG382000 Rh6DG368500
rosa_wichuraiana Rw6G032090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 788
AccB7I CCANNNNNTGG 1 cut(s) 174
AccII CGCG 3 cut(s) 111, 474, 843
AccIII TCCGGA 1 cut(s) 157
AciI CCGC 7 cut(s) 109, 111, 233, 256, 350, 767, 795
AclWI GGATC 1 cut(s) 596
AcoI YGGCCR 3 cut(s) 165, 304, 362
AcsI RAATTY 5 cut(s) 332, 824, 837, 859, 869
AcuI CTGAAG 1 cut(s) 885
AcyI GRCGYC 2 cut(s) 106, 190
AfaI GTAC 1 cut(s) 749
AfiI CCNNNNNNNGG 3 cut(s) 174, 252, 534
AgsI TTSAA 3 cut(s) 322, 858, 882
AjiI CACGTC 1 cut(s) 64
AluBI AGCT 5 cut(s) 98, 214, 440, 492, 514
AluI AGCT 5 cut(s) 98, 214, 440, 492, 514
Alw21I GWGCWC 1 cut(s) 100
Alw26I GTCTC 3 cut(s) 351, 375, 396
AlwI GGATC 1 cut(s) 596
Aor13HI TCCGGA 1 cut(s) 157
AoxI GGCC 4 cut(s) 165, 304, 362, 874
ApoI RAATTY 5 cut(s) 332, 824, 837, 859, 869
Asp700I GAANNNNTTC 2 cut(s) 42, 413
AspLEI GCGC 2 cut(s) 476, 845
AspS9I GGNCC 1 cut(s) 875
BalI TGGCCA 1 cut(s) 306
BanII GRGCYC 1 cut(s) 100
Bbv12I GWGCWC 1 cut(s) 100
BccI CCATC 3 cut(s) 281, 391, 453
BceAI ACGGC 5 cut(s) 35, 180, 203, 361, 377
BciVI GTATCC 2 cut(s) 206, 253
BcoDI GTCTC 3 cut(s) 351, 375, 396
BfuAI ACCTGC 1 cut(s) 788
BfuI GTATCC 2 cut(s) 206, 253
BisI GCNGC 1 cut(s) 109
BlsI GCNGC 1 cut(s) 110
BmgBI CACGTC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 875
BmiI GGNNCC 1 cut(s) 179
Bsa29I ATCGAT 1 cut(s) 609
BsaHI GRCGYC 2 cut(s) 106, 190
BsaJI CCNNGG 1 cut(s) 109
BsaWI WCCGGW 3 cut(s) 157, 198, 534
Bsc4I CCNNNNNNNGG 3 cut(s) 174, 252, 534
BseAI TCCGGA 1 cut(s) 157
BseCI ATCGAT 1 cut(s) 609
BseDI CCNNGG 1 cut(s) 109
BseGI GGATG 4 cut(s) 273, 402, 445, 791
BseLI CCNNNNNNNGG 3 cut(s) 174, 252, 534
BseMII CTCAG 2 cut(s) 386, 641
BseRI GAGGAG 1 cut(s) 448
BseYI CCCAGC 1 cut(s) 210
Bsh1236I CGCG 3 cut(s) 111, 474, 843
Bsh1285I CGRYCG 1 cut(s) 203
BshFI GGCC 4 cut(s) 167, 306, 364, 876
BshVI ATCGAT 1 cut(s) 609
BsiEI CGRYCG 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 100
BsiSI CCGG 3 cut(s) 158, 199, 535
BslI CCNNNNNNNGG 3 cut(s) 174, 252, 534
BsmAI GTCTC 3 cut(s) 351, 375, 396
BsmBI CGTCTC 2 cut(s) 375, 396
BsmI GAATGC 1 cut(s) 44
BsnI GGCC 4 cut(s) 167, 306, 364, 876
Bsp1286I GDGCHC 1 cut(s) 100
Bsp13I TCCGGA 1 cut(s) 157
Bsp143I GATC 1 cut(s) 588
BspACI CCGC 7 cut(s) 109, 111, 233, 256, 350, 767, 795
BspANI GGCC 4 cut(s) 167, 306, 364, 876
BspCNI CTCAG 2 cut(s) 385, 640
BspDI ATCGAT 1 cut(s) 609
BspEI TCCGGA 1 cut(s) 157
BspFNI CGCG 3 cut(s) 111, 474, 843
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 1 cut(s) 179
BspMI ACCTGC 1 cut(s) 788
BspPI GGATC 1 cut(s) 596
BspQI GCTCTTC 1 cut(s) 272
BssECI CCNNGG 1 cut(s) 109
BssMI GATC 1 cut(s) 588
BssNI GRCGYC 2 cut(s) 106, 190
Bst4CI ACNGT 6 cut(s) 22, 228, 314, 328, 357, 369
Bst6I CTCTTC 3 cut(s) 86, 272, 466
BstACI GRCGYC 2 cut(s) 106, 190
BstC8I GCNNGC 1 cut(s) 100
BstDEI CTNAG 4 cut(s) 372, 521, 627, 663
BstDSI CCRYGG 1 cut(s) 109
BstEII GGTNACC 1 cut(s) 719
BstF5I GGATG 4 cut(s) 273, 402, 445, 791
BstFNI CGCG 3 cut(s) 111, 474, 843
BstHHI GCGC 2 cut(s) 476, 845
BstKTI GATC 1 cut(s) 591
BstMAI GTCTC 3 cut(s) 351, 375, 396
BstMBI GATC 1 cut(s) 588
BstMCI CGRYCG 1 cut(s) 203
BstMWI GCNNNNNNNGC 3 cut(s) 108, 220, 262
BstPI GGTNACC 1 cut(s) 719
BstUI CGCG 3 cut(s) 111, 474, 843
Bsu15I ATCGAT 1 cut(s) 609
BsuI GTATCC 2 cut(s) 206, 253
BsuRI GGCC 4 cut(s) 167, 306, 364, 876
BsuTUI ATCGAT 1 cut(s) 609
BtgI CCRYGG 1 cut(s) 109
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 4 cut(s) 273, 402, 445, 791
BtsIMutI CAGTG 2 cut(s) 18, 224
BveI ACCTGC 1 cut(s) 788
Cac8I GCNNGC 1 cut(s) 100
CciI TCATGA 1 cut(s) 802
CfoI GCGC 2 cut(s) 476, 845
Cfr13I GGNCC 1 cut(s) 875
Cfr42I CCGCGG 1 cut(s) 112
ClaI ATCGAT 1 cut(s) 609
CseI GACGC 3 cut(s) 114, 179, 378
Csp6I GTAC 1 cut(s) 748
CviAII CATG 2 cut(s) 672, 803
CviQI GTAC 1 cut(s) 748
DdeI CTNAG 4 cut(s) 372, 521, 627, 663
DpnI GATC 1 cut(s) 590
DpnII GATC 1 cut(s) 588
EaeI YGGCCR 3 cut(s) 165, 304, 362
Eam1104I CTCTTC 3 cut(s) 86, 272, 466
EarI CTCTTC 3 cut(s) 86, 272, 466
EciI GGCGGA 1 cut(s) 339
Ecl136II GAGCTC 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 100
Eco53kI GAGCTC 1 cut(s) 98
Eco57I CTGAAG 1 cut(s) 885
Eco91I GGTNACC 1 cut(s) 719
EcoICRI GAGCTC 1 cut(s) 98
EcoO65I GGTNACC 1 cut(s) 719
EcoRI GAATTC 1 cut(s) 837
EcoT38I GRGCYC 1 cut(s) 100
Esp3I CGTCTC 2 cut(s) 375, 396
FaeI CATG 2 cut(s) 675, 806
FatI CATG 2 cut(s) 671, 802
FauI CCCGC 2 cut(s) 249, 774
Fnu4HI GCNGC 1 cut(s) 109
FokI GGATG 4 cut(s) 260, 409, 432, 778
FriOI GRGCYC 1 cut(s) 100
Fsp4HI GCNGC 1 cut(s) 109
GlaI GCGC 2 cut(s) 475, 844
GluI GCNGC 1 cut(s) 109
GsaI CCCAGC 1 cut(s) 214
HaeIII GGCC 4 cut(s) 167, 306, 364, 876
HapII CCGG 3 cut(s) 158, 199, 535
HgaI GACGC 3 cut(s) 114, 179, 378
HhaI GCGC 2 cut(s) 476, 845
Hin1I GRCGYC 2 cut(s) 106, 190
Hin1II CATG 2 cut(s) 675, 806
Hin6I GCGC 2 cut(s) 474, 843
HinP1I GCGC 2 cut(s) 474, 843
HinfI GANTC 4 cut(s) 114, 249, 782, 788
HpaII CCGG 3 cut(s) 158, 199, 535
Hpy166II GTNNAC 4 cut(s) 61, 85, 650, 750
Hpy188I TCNGA 5 cut(s) 340, 396, 419, 787, 865
Hpy188III TCNNGA 3 cut(s) 158, 181, 803
Hpy8I GTNNAC 4 cut(s) 61, 85, 650, 750
Hpy99I CGWCG 1 cut(s) 195
HpyAV CCTTC 5 cut(s) 53, 459, 463, 864, 888
HpyCH4III ACNGT 6 cut(s) 22, 228, 314, 328, 357, 369
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 3 cut(s) 129, 819, 850
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 220, 262
HpyF3I CTNAG 4 cut(s) 372, 521, 627, 663
HpySE526I ACGT 1 cut(s) 63
Hsp92I GRCGYC 2 cut(s) 106, 190
Hsp92II CATG 2 cut(s) 675, 806
HspAI GCGC 2 cut(s) 474, 843
Kpn2I TCCGGA 1 cut(s) 157
KspI CCGCGG 1 cut(s) 112
Kzo9I GATC 1 cut(s) 588
LguI GCTCTTC 1 cut(s) 272
LmnI GCTCC 2 cut(s) 219, 437
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 5 cut(s) 22, 357, 425, 719, 808
MalI GATC 1 cut(s) 590
MboI GATC 1 cut(s) 588
MboII GAAGA 8 cut(s) 103, 142, 259, 453, 456, 459, 702, 878
MhlI GDGCHC 1 cut(s) 100
MlsI TGGCCA 1 cut(s) 306
MluCI AATT 9 cut(s) 130, 316, 332, 484, 824, 837, 859, 869, 882
MluNI TGGCCA 1 cut(s) 306
MlyI GAGTC 1 cut(s) 123
MmeI TCCRAC 1 cut(s) 241
MnlI CCTC 6 cut(s) 24, 310, 413, 440, 469, 578
Mox20I TGGCCA 1 cut(s) 306
MroI TCCGGA 1 cut(s) 157
MroXI GAANNNNTTC 2 cut(s) 42, 413
MscI TGGCCA 1 cut(s) 306
MseI TTAA 4 cut(s) 54, 237, 603, 642
Msp20I TGGCCA 1 cut(s) 306
MspA1I CMGCKG 2 cut(s) 111, 492
MspI CCGG 3 cut(s) 158, 199, 535
Mva1269I GAATGC 1 cut(s) 44
MvnI CGCG 3 cut(s) 111, 474, 843
MwoI GCNNNNNNNGC 3 cut(s) 108, 220, 262
NdeII GATC 1 cut(s) 588
NlaIII CATG 2 cut(s) 675, 806
NlaIV GGNNCC 1 cut(s) 179
NmeAIII GCCGAG 1 cut(s) 122
NmuCI GTSAC 2 cut(s) 22, 357
PagI TCATGA 1 cut(s) 802
PciSI GCTCTTC 1 cut(s) 272
PctI GAATGC 1 cut(s) 44
PdmI GAANNNNTTC 2 cut(s) 42, 413
PfeI GAWTC 3 cut(s) 249, 782, 788
PflMI CCANNNNNTGG 1 cut(s) 174
PkrI GCNGC 1 cut(s) 110
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
Psp124BI GAGCTC 1 cut(s) 100
PspEI GGTNACC 1 cut(s) 719
PspFI CCCAGC 1 cut(s) 210
PspN4I GGNNCC 1 cut(s) 179
PspPI GGNCC 1 cut(s) 875
PvuII CAGCTG 1 cut(s) 492
RsaI GTAC 1 cut(s) 749
RsaNI GTAC 1 cut(s) 748
SacI GAGCTC 1 cut(s) 100
SacII CCGCGG 1 cut(s) 112
SapI GCTCTTC 1 cut(s) 272
SaqAI TTAA 4 cut(s) 54, 237, 603, 642
SatI GCNGC 1 cut(s) 109
Sau3AI GATC 1 cut(s) 588
Sau96I GGNCC 1 cut(s) 875
SchI GAGTC 1 cut(s) 123
SduI GDGCHC 1 cut(s) 100
Sfr303I CCGCGG 1 cut(s) 112
SgrBI CCGCGG 1 cut(s) 112
Sse9I AATT 9 cut(s) 130, 316, 332, 484, 824, 837, 859, 869, 882
SsiI CCGC 7 cut(s) 109, 111, 233, 256, 350, 767, 795
SspI AATATT 1 cut(s) 412
SstI GAGCTC 1 cut(s) 100
TaaI ACNGT 6 cut(s) 22, 228, 314, 328, 357, 369
TaiI ACGT 1 cut(s) 66
TaqI TCGA 4 cut(s) 203, 330, 407, 609
TasI AATT 9 cut(s) 130, 316, 332, 484, 824, 837, 859, 869, 882
TatI WGTACW 1 cut(s) 747
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 3 cut(s) 249, 782, 788
Tru1I TTAA 4 cut(s) 54, 237, 603, 642
Tru9I TTAA 4 cut(s) 54, 237, 603, 642
TscAI CASTG 2 cut(s) 25, 231
TseFI GTSAC 2 cut(s) 22, 357
Tsp45I GTSAC 2 cut(s) 22, 357
TspDTI ATGAA 8 cut(s) 123, 127, 651, 660, 744, 780, 795, 819
TspGWI ACGGA 1 cut(s) 450
TspRI CASTG 2 cut(s) 25, 231
Van91I CCANNNNNTGG 1 cut(s) 174
XapI RAATTY 5 cut(s) 332, 824, 837, 859, 869
XmnI GAANNNNTTC 2 cut(s) 42, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.