pycom08g14960

protein At4g37920, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014976
Physical Location & Seq
Reverse (-)
14417733 .. 14427398
9666 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2613 bp
ATGGAGGTTTCCACCGCCACCCTCCAACCGTCTTGTTCTTTCCCACTCAGAGCCCCTAAACCCGCCACCAGAGAACGCGTCTCTTCTCTCTCCTCCATCAGATCCTCCCCTAAATCTATCTCCGTATCTTATAGCATCACCAAAGGTTGTGACTTAGTAGTTAGAGAACCAGTTAAGTTACGGTGGTCCTCTGTTGTTGCTGCTACGGTTGGTGATACAACTGCAGTGCCAAATAGTTGCTCTAGTGGAGAAGAGTTGCCCGTTTCTGATTCTTCTAATCCCATATCAGTGGAAGATCAGCCCAGTGGAAATGGTGGGAAAGGGGAAGAGAAAGGTGGGATTGGGGGATTGGATGACCACAAGATGGCCCGAATATGTGACAAGCTTATTGAGGTTTTCTTGGTTGACAAGCCCACTCCAACTGACTGGAGAAGATTATTAGCTTTCAGTAAGGAATGGGATGATATCCGGCCTCATTTCTATGCGCGGTGTCAGGACCGCGCAGATGCTGAGGATGATCCTGGAATGAAGCATAAGCTACTACGGCTTGGACGGAAATTGAAAGAGATTGATGAAGATGTGCAAAGACACAATGAACTTCTTAAAGTCGTTAGAGGGGCTCAATCAGAAATTAGTGAAATTGTTTCCAGACGTCGTAAAGATTTCACTCAAGAATTTTTTGTGCATCTCCACACTGTTGCCGAATCCTATTACAACAATCCGACAGAGCAAACTGCTTTGGCAAGTATTGGAAATATGTGCTTGGCTGCTGTGCAAGCATATGATTCTGCAACTGAAAGCATTGAAGCACTGAATGCTGCAGAGCTGAAATTTCAAGATATTATCGACTCTCCAACTGTTGATGCGGCTTGTAGGAAGATAGATCGCTTGGCTGAGAAAAATCAACTTGACTCAGCATTGGTGATGATGATCACAAAAGCTTGGTCAGCTGCCAAAGAGTCGAGCATGACGAAAGATGAGGTAAAGGACGTACTTTATCATTTGTACGTGACCGCAAGAGGTAATCTTCAGAGACTCATGCCAAAAGAAATTCGGATACTTAAGTATCTTCTCACAATCGAGGATCCTGAGGAGCGTCTGGCTGCCTTACACGATGCATTTACCCCAGGGGAGGAACTTGAAGGGACGGATGTGGATAACCTTTTCACGACCCCGGTGCAGCTTCACACCTGGATGAAGGCTATTGTGGACGCTTACCATTTCAGCCAGGAAGGCACTCTAATAAGGGAAGCAAGAGACCTCATGAATCCAAAGATCATTCAAAAAATGGAGGAGCTGAAGAAGTTAGTTGAAAACAAGTTCCTGTTGATTCGCTGTTTCGGAGGGATGAACCGGAAATCTAGTAATTGTGCTATTTGTGAGAATTCAAATCTTGCTTCTATCTGTGCTGTTTGTGTGAATTACAGATTAAATGAGTATAACAATTCACTGAAGGCACTGAAAAGTCGTCGCGATTCATTGTATTCAACATTGACTGAAGTGCTTGTCGCAAAGGGTAAGGCCGATGATCAAATAAATTGGAGACTGCTTCAAAATGAGAAGCTTGTGAGGTTAAGGGAAAAGCTCCGGCGTAATAAAGAACAGCTTGTGCAAGGGAAGGCTAAGATTGAGAAGGCGTCCCATGACCGTAAAGTCAAGGATGGGGTGCTTGAATCAGCCCTCTTTGTGTTGGGAAAGAATCGTGCGGAACAACTGGATAAGTTCTATCCCAACCTTATATACACTCAGAACTTAGGGCATATGGCCATTACCTCTGAACGCCTTCATAAACAGTCAGTAGTTATAAAACAAATATGCAAATTGTTTCCCCAACGTCGGGTGATTGTAGATGAAAAGAAAAATTATGGATCTGGTGGTTTATACGATCAAATTTGCAATACATGCTTACCAAGAGGGCTAGATCCACACTCAGTTCCATCAGAAGAGCTTGCTGCTTCCTTGGGGTACATGGTCCAACTCCTGAATCTTGTTGTTCAGAACTTAGCTGCTCCAGCACTTCATAACTCAGGTTTTGCGGGTTCTTGCTCTAGGATATGGCAAAGAGATTCTTATTGGAATGCGTGCCCTTCTTCTCGGAGCAATGAATATCCCCTATTTATACCACGCCAAAATTATTGCTCCAGTATTGGGGAAAATTCTTGGTCTGACAAAAGCTCAAGTAATAATTTTGGTGTTGATTCAATAGAATCTGTGAGGAAACCACATCTGGATTCTTCTGGTGGTAGCTTTAATTACACTTCCGCTTCTTCACACACTGTTGAAACACACAATGATTTGCAGAGAGGGATTTCACTTCTTAAGAAAAGTGTCGCATGCATTACAGCGTACTGCTATAACTCACTATGTTTAGATGTTCCTTCTGAGTTATCTACATTTGAAGCATTTGCAAAATTGTTGGCCACATTATCTTCTTCCAAGGAAGTTCATTCTGTGTTCTCGTTAAAATTGGCTTGCTCAAGGTCATGTAAGCAAGTTCAGCAACTAAACAAATCTGTGTGGAATGTGGATTCGGCCATATCGTCAACCACTCTATTGGATAGCACACATGCAATGGCACTAACGAAAGAACTTGTATTCCACCACCTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

871

Amino Acids

97.28

Weight (kDa)

8.05

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG14 PF10186 457 - 814 1e-81 Vacuolar sorting 38 and autophagy-related subunit 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G36320
fragaria_vesca FvH4_5g38330 FvH4_5g38330 FvH4_5g38330
malus_domestica MD08G1174600.v1.1 MD15G1361200.v1.1
prunus_persica Prupe.1G508600_v2.0.a1 Prupe.1G508600_v2.0.a1
pyrus_communis pycom08g14960
rosa_chinensis RchiOBHm_Chr7g0240351
rosa_multiflora Rmu_sc0038179.1_g000001
rosa_roxburghii Rroxscaffold_3G00220200
rosa_samantha Rh7AG489300 Rh7BG462300 Rh7CG505600
rosa_wichuraiana Rw7G040940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1805
AasI GACNNNNNNGTC 1 cut(s) 1652
AatII GACGTC 1 cut(s) 655
AccB7I CCANNNNNTGG 1 cut(s) 364
AccII CGCG 4 cut(s) 78, 487, 501, 1473
AciI CCGC 9 cut(s) 15, 63, 487, 499, 866, 1014, 1706, 2036, 2262
AclWI GGATC 6 cut(s) 96, 512, 1079, 1092, 1876, 1916
AcoI YGGCCR 3 cut(s) 1764, 2418, 2532
AcsI RAATTY 6 cut(s) 674, 830, 1050, 1384, 1890, 2155
AcuI CTGAAG 4 cut(s) 1013, 1319, 1472, 1518
AcyI GRCGYC 2 cut(s) 652, 1637
AfaI GTAC 4 cut(s) 993, 1007, 1967, 2348
AfiI CCNNNNNNNGG 5 cut(s) 364, 1132, 1836, 1837, 2148
AflII CTTAAG 2 cut(s) 1061, 2318
AflIII ACRYGT 1 cut(s) 76
AjnI CCWGG 4 cut(s) 520, 1126, 1190, 1227
AloI GAACNNNNNNTCC 2 cut(s) 2580, 2612
Alw26I GTCTC 4 cut(s) 85, 1027, 1251, 1537
AlwI GGATC 6 cut(s) 96, 512, 1079, 1092, 1876, 1916
AlwNI CAGNNNCTG 1 cut(s) 509
AoxI GGCC 6 cut(s) 366, 470, 1521, 1764, 2418, 2532
ApeKI GCWGC 8 cut(s) 200, 767, 818, 950, 1103, 1180, 1952, 2006
ApoI RAATTY 6 cut(s) 674, 830, 1050, 1384, 1890, 2155
ArsI GACNNNNNNTTYG 2 cut(s) 929, 961
Asp700I GAANNNNTTC 1 cut(s) 1782
AspLEI GCGC 2 cut(s) 487, 503
AspS9I GGNCC 4 cut(s) 186, 367, 496, 1972
AsuC2I CCSGG 1 cut(s) 1175
AsuHPI GGTGA 4 cut(s) 130, 224, 934, 1852
AvaII GGWCC 3 cut(s) 186, 496, 1972
AxyI CCTNAGG 1 cut(s) 1089
BaeGI GKGCMC 1 cut(s) 2087
BaeI ACNNNNGTAYC 4 cut(s) 1049, 1049, 1082, 1082
BalI TGGCCA 2 cut(s) 1766, 2420
BamHI GGATCC 1 cut(s) 1084
BanII GRGCYC 2 cut(s) 55, 622
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 8 cut(s) 187, 754, 805, 937, 1090, 1192, 1939, 1993
BccI CCATC 4 cut(s) 104, 358, 1655, 1945
BceAI ACGGC 1 cut(s) 560
BcgI CGANNNNNNTGC 2 cut(s) 1159, 1193
BciT130I CCWGG 4 cut(s) 522, 1128, 1192, 1229
BciVI GTATCC 1 cut(s) 1050
BclI TGATCA 2 cut(s) 930, 1528
BcnI CCSGG 1 cut(s) 1175
BcoDI GTCTC 4 cut(s) 85, 1027, 1251, 1537
BfaI CTAG 4 cut(s) 243, 1362, 1919, 2049
BfmI CTRYAG 2 cut(s) 222, 819
BfrI CTTAAG 2 cut(s) 1061, 2318
BfuI GTATCC 1 cut(s) 1050
BglI GCCNNNNNGGC 1 cut(s) 1233
BisI GCNGC 9 cut(s) 201, 768, 819, 867, 951, 1104, 1181, 1953, 2007
BlsI GCNGC 9 cut(s) 202, 769, 820, 868, 952, 1105, 1182, 1954, 2008
Bme1390I CCNGG 5 cut(s) 522, 1128, 1175, 1192, 1229
Bme18I GGWCC 3 cut(s) 186, 496, 1972
BmgT120I GGNCC 4 cut(s) 186, 367, 496, 1972
BmiI GGNNCC 1 cut(s) 1086
BmrFI CCNGG 5 cut(s) 522, 1128, 1175, 1192, 1229
BmrI ACTGGG 1 cut(s) 297
BmsI GCATC 5 cut(s) 144, 496, 694, 853, 1105
BmuI ACTGGG 1 cut(s) 297
BpmI CTGGAG 3 cut(s) 448, 1995, 2125
Bpu10I CCTNAGC 1 cut(s) 510
BpuEI CTTGAG 3 cut(s) 654, 2161, 2461
BpuMI CCSGG 1 cut(s) 1175
BsaAI YACGTR 1 cut(s) 1009
BsaBI GATNNNNATC 1 cut(s) 929
BsaHI GRCGYC 2 cut(s) 652, 1637
BsaI GGTCTC 1 cut(s) 1251
BsaJI CCNNGG 5 cut(s) 1126, 1127, 1173, 1959, 2436
BsaWI WCCGGW 1 cut(s) 1353
Bsc4I CCNNNNNNNGG 5 cut(s) 364, 1132, 1836, 1837, 2148
Bse1I ACTGG 5 cut(s) 170, 303, 431, 1719, 2142
Bse21I CCTNAGG 1 cut(s) 1089
Bse3DI GCAATG 2 cut(s) 2107, 2577
Bse8I GATNNNNATC 1 cut(s) 929
BseBI CCWGG 4 cut(s) 522, 1128, 1192, 1229
BseDI CCNNGG 5 cut(s) 1126, 1127, 1173, 1959, 2436
BseGI GGATG 7 cut(s) 358, 466, 520, 1156, 1200, 1353, 1666
BseJI GATNNNNATC 1 cut(s) 929
BseLI CCNNNNNNNGG 5 cut(s) 364, 1132, 1836, 1837, 2148
BseMI GCAATG 2 cut(s) 2107, 2577
BseMII CTCAG 9 cut(s) 61, 501, 885, 927, 1080, 1760, 1944, 2040, 2373
BseNI ACTGG 5 cut(s) 170, 303, 431, 1719, 2142
BseRI GAGGAG 3 cut(s) 82, 1106, 1307
BseSI GKGCMC 1 cut(s) 2087
BseXI GCAGC 8 cut(s) 187, 754, 805, 937, 1090, 1192, 1939, 1993
BsgI GTGCAG 1 cut(s) 1199
Bsh1236I CGCG 4 cut(s) 78, 487, 501, 1473
BshFI GGCC 6 cut(s) 368, 472, 1523, 1766, 2420, 2534
BsiSI CCGG 4 cut(s) 469, 1175, 1354, 1588
BslFI GGGAC 2 cut(s) 1159, 1624
BslI CCNNNNNNNGG 5 cut(s) 364, 1132, 1836, 1837, 2148
BsmAI GTCTC 4 cut(s) 85, 1027, 1251, 1537
BsmBI CGTCTC 1 cut(s) 85
BsmFI GGGAC 2 cut(s) 1159, 1624
BsmI GAATGC 2 cut(s) 820, 2083
BsnI GGCC 6 cut(s) 368, 472, 1523, 1766, 2420, 2534
Bso31I GGTCTC 1 cut(s) 1251
Bsp1286I GDGCHC 3 cut(s) 55, 622, 2087
Bsp68I TCGCGA 1 cut(s) 1473
BspACI CCGC 9 cut(s) 15, 63, 487, 499, 866, 1014, 1706, 2036, 2262
BspANI GGCC 6 cut(s) 368, 472, 1523, 1766, 2420, 2534
BspCNI CTCAG 9 cut(s) 60, 502, 886, 926, 1081, 1759, 1943, 2039, 2374
BspFNI CGCG 4 cut(s) 78, 487, 501, 1473
BspHI TCATGA 1 cut(s) 1263
BspLI GGNNCC 1 cut(s) 1086
BspMAI CTGCAG 2 cut(s) 226, 823
BspPI GGATC 6 cut(s) 96, 512, 1079, 1092, 1876, 1916
BspQI GCTCTTC 1 cut(s) 1938
BspTI CTTAAG 2 cut(s) 1061, 2318
BspTNI GGTCTC 1 cut(s) 1251
BsrDI GCAATG 2 cut(s) 2107, 2577
BsrI ACTGG 5 cut(s) 170, 303, 431, 1719, 2142
BssECI CCNNGG 5 cut(s) 1126, 1127, 1173, 1959, 2436
BssNI GRCGYC 2 cut(s) 652, 1637
BssT1I CCWWGG 2 cut(s) 1959, 2436
Bst2UI CCWGG 4 cut(s) 522, 1128, 1192, 1229
Bst4CI ACNGT 8 cut(s) 30, 183, 208, 697, 859, 1649, 1794, 2278
Bst6I CTCTTC 4 cut(s) 88, 246, 321, 1938
BstACI GRCGYC 2 cut(s) 652, 1637
BstAFI CTTAAG 2 cut(s) 1061, 2318
BstAPI GCANNNNNTGC 2 cut(s) 815, 1902
BstBAI YACGTR 1 cut(s) 1009
BstC8I GCNNGC 5 cut(s) 777, 1950, 2083, 2335, 2473
BstF5I GGATG 7 cut(s) 358, 466, 520, 1156, 1200, 1353, 1666
BstFNI CGCG 4 cut(s) 78, 487, 501, 1473
BstHHI GCGC 2 cut(s) 487, 503
BstMAI GTCTC 4 cut(s) 85, 1027, 1251, 1537
BstMWI GCNNNNNNNGC 8 cut(s) 544, 776, 815, 947, 1233, 1902, 2012, 2497
BstNI CCWGG 4 cut(s) 522, 1128, 1192, 1229
BstNSI RCATGY 3 cut(s) 1905, 2337, 2570
BstSCI CCNGG 5 cut(s) 520, 1126, 1173, 1190, 1227
BstSFI CTRYAG 2 cut(s) 222, 819
BstSLI GKGCMC 1 cut(s) 2087
BstUI CGCG 4 cut(s) 78, 487, 501, 1473
BstV1I GCAGC 8 cut(s) 187, 754, 805, 937, 1090, 1192, 1939, 1993
BstX2I RGATCY 4 cut(s) 101, 1084, 1868, 1921
BstXI CCANNNNNNTGG 3 cut(s) 289, 426, 2554
BstYI RGATCY 4 cut(s) 101, 1084, 1868, 1921
Bsu36I CCTNAGG 1 cut(s) 1089
BsuI GTATCC 1 cut(s) 1050
BsuRI GGCC 6 cut(s) 368, 472, 1523, 1766, 2420, 2534
BtsCI GGATG 7 cut(s) 358, 466, 520, 1156, 1200, 1353, 1666
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 8 cut(s) 231, 294, 310, 693, 809, 1448, 1457, 2274
BtuMI TCGCGA 1 cut(s) 1473
Cac8I GCNNGC 5 cut(s) 777, 1950, 2083, 2335, 2473
CaiI CAGNNNCTG 1 cut(s) 509
CciI TCATGA 1 cut(s) 1263
CfoI GCGC 2 cut(s) 487, 503
Cfr13I GGNCC 4 cut(s) 186, 367, 496, 1972
CseI GACGC 4 cut(s) 67, 1085, 1220, 1626
Csp6I GTAC 4 cut(s) 992, 1006, 1966, 2347
CviAII CATG 9 cut(s) 967, 1039, 1264, 1643, 1902, 1969, 2334, 2484, 2567
CviQI GTAC 4 cut(s) 992, 1006, 1966, 2347
DrdI GACNNNNNNGTC 1 cut(s) 1652
DseDI GACNNNNNNGTC 1 cut(s) 1652
EaeI YGGCCR 3 cut(s) 1764, 2418, 2532
Eam1104I CTCTTC 4 cut(s) 88, 246, 321, 1938
EarI CTCTTC 4 cut(s) 88, 246, 321, 1938
Eco130I CCWWGG 2 cut(s) 1959, 2436
Eco24I GRGCYC 2 cut(s) 55, 622
Eco31I GGTCTC 1 cut(s) 1251
Eco32I GATATC 1 cut(s) 466
Eco47I GGWCC 3 cut(s) 186, 496, 1972
Eco57I CTGAAG 4 cut(s) 1013, 1319, 1472, 1518
Eco81I CCTNAGG 1 cut(s) 1089
EcoRI GAATTC 1 cut(s) 1384
EcoRII CCWGG 4 cut(s) 520, 1126, 1190, 1227
EcoRV GATATC 1 cut(s) 466
EcoT14I CCWWGG 2 cut(s) 1959, 2436
EcoT22I ATGCAT 2 cut(s) 1120, 2339
EcoT38I GRGCYC 2 cut(s) 55, 622
ErhI CCWWGG 2 cut(s) 1959, 2436
Esp3I CGTCTC 1 cut(s) 85
FaeI CATG 9 cut(s) 970, 1042, 1267, 1646, 1905, 1972, 2337, 2487, 2570
FalI AAGNNNNNCTT 4 cut(s) 1590, 1622, 2053, 2085
FaqI GGGAC 2 cut(s) 1159, 1624
FatI CATG 9 cut(s) 966, 1038, 1263, 1642, 1901, 1968, 2333, 2483, 2566
FauI CCCGC 2 cut(s) 70, 2029
FauNDI CATATG 2 cut(s) 781, 1761
FbaI TGATCA 2 cut(s) 930, 1528
Fnu4HI GCNGC 9 cut(s) 201, 768, 819, 867, 951, 1104, 1181, 1953, 2007
FokI GGATG 7 cut(s) 365, 473, 527, 1163, 1207, 1360, 1673
FriOI GRGCYC 2 cut(s) 55, 622
Fsp4HI GCNGC 9 cut(s) 201, 768, 819, 867, 951, 1104, 1181, 1953, 2007
FspBI CTAG 4 cut(s) 243, 1362, 1919, 2049
GlaI GCGC 2 cut(s) 486, 502
GluI GCNGC 9 cut(s) 201, 768, 819, 867, 951, 1104, 1181, 1953, 2007
GsuI CTGGAG 3 cut(s) 448, 1995, 2125
HaeIII GGCC 6 cut(s) 368, 472, 1523, 1766, 2420, 2534
HapII CCGG 4 cut(s) 469, 1175, 1354, 1588
HgaI GACGC 4 cut(s) 67, 1085, 1220, 1626
HhaI GCGC 2 cut(s) 487, 503
Hin1I GRCGYC 2 cut(s) 652, 1637
Hin1II CATG 9 cut(s) 970, 1042, 1267, 1646, 1905, 1972, 2337, 2487, 2570
Hin6I GCGC 2 cut(s) 485, 501
HinP1I GCGC 2 cut(s) 485, 501
HincII GTYRAC 2 cut(s) 406, 2544
HindII GTYRAC 2 cut(s) 406, 2544
HindIII AAGCTT 3 cut(s) 383, 939, 1562
HpaII CCGG 4 cut(s) 469, 1175, 1354, 1588
HphI GGTGA 4 cut(s) 130, 224, 934, 1852
Hpy166II GTNNAC 3 cut(s) 406, 1210, 2544
Hpy8I GTNNAC 3 cut(s) 406, 1210, 2544
Hpy99I CGWCG 3 cut(s) 657, 1473, 1839
HpyAV CCTTC 9 cut(s) 1136, 1192, 1226, 1447, 1612, 1627, 1793, 2097, 2388
HpyCH4III ACNGT 8 cut(s) 30, 183, 208, 697, 859, 1649, 1794, 2278
HpyCH4IV ACGT 4 cut(s) 652, 990, 1008, 1834
HpyF10VI GCNNNNNNNGC 8 cut(s) 544, 776, 815, 947, 1233, 1902, 2012, 2497
HpySE526I ACGT 4 cut(s) 652, 990, 1008, 1834
Hsp92I GRCGYC 2 cut(s) 652, 1637
Hsp92II CATG 9 cut(s) 970, 1042, 1267, 1646, 1905, 1972, 2337, 2487, 2570
HspAI GCGC 2 cut(s) 485, 501
Ksp22I TGATCA 2 cut(s) 930, 1528
LguI GCTCTTC 1 cut(s) 1938
LmnI GCTCC 6 cut(s) 1093, 1294, 1590, 2014, 2097, 2144
Lsp1109I GCAGC 8 cut(s) 187, 754, 805, 937, 1090, 1192, 1939, 1993
LweI GCATC 5 cut(s) 144, 496, 694, 853, 1105
MaeI CTAG 4 cut(s) 243, 1362, 1919, 2049
MaeII ACGT 4 cut(s) 652, 990, 1008, 1834
MaeIII GTNAC 4 cut(s) 149, 177, 377, 1009
MflI RGATCY 4 cut(s) 101, 1084, 1868, 1921
MhlI GDGCHC 3 cut(s) 55, 622, 2087
MlsI TGGCCA 2 cut(s) 1766, 2420
MluI ACGCGT 1 cut(s) 76
MluNI TGGCCA 2 cut(s) 1766, 2420
MlyI GAGTC 4 cut(s) 842, 905, 968, 1029
MmeI TCCRAC 5 cut(s) 49, 443, 746, 878, 1999
Mox20I TGGCCA 2 cut(s) 1766, 2420
Mph1103I ATGCAT 2 cut(s) 1120, 2339
MroXI GAANNNNTTC 1 cut(s) 1782
MscI TGGCCA 2 cut(s) 1766, 2420
MseI TTAA 8 cut(s) 174, 603, 1062, 1430, 1574, 2250, 2319, 2462
MslI CAYNNNNRTG 3 cut(s) 287, 480, 1193
Msp20I TGGCCA 2 cut(s) 1766, 2420
MspA1I CMGCKG 1 cut(s) 950
MspCI CTTAAG 2 cut(s) 1061, 2318
MspI CCGG 4 cut(s) 469, 1175, 1354, 1588
MspR9I CCNGG 5 cut(s) 522, 1128, 1175, 1192, 1229
Mva1269I GAATGC 2 cut(s) 820, 2083
MvaI CCWGG 4 cut(s) 522, 1128, 1192, 1229
MvnI CGCG 4 cut(s) 78, 487, 501, 1473
MwoI GCNNNNNNNGC 8 cut(s) 544, 776, 815, 947, 1233, 1902, 2012, 2497
NciI CCSGG 1 cut(s) 1175
NdeI CATATG 2 cut(s) 781, 1761
NlaIII CATG 9 cut(s) 970, 1042, 1267, 1646, 1905, 1972, 2337, 2487, 2570
NlaIV GGNNCC 1 cut(s) 1086
NmuCI GTSAC 3 cut(s) 149, 377, 1009
NruI TCGCGA 1 cut(s) 1473
NsiI ATGCAT 2 cut(s) 1120, 2339
NspI RCATGY 3 cut(s) 1905, 2337, 2570
PaeI GCATGC 1 cut(s) 2337
PagI TCATGA 1 cut(s) 1263
PasI CCCWGGG 1 cut(s) 1127
PciSI GCTCTTC 1 cut(s) 1938
PcsI WCGNNNNNNNCGW 2 cut(s) 968, 2537
PctI GAATGC 2 cut(s) 820, 2083
PdmI GAANNNNTTC 1 cut(s) 1782
PflMI CCANNNNNTGG 1 cut(s) 364
PfoI TCCNGGA 1 cut(s) 520
PkrI GCNGC 9 cut(s) 202, 769, 820, 868, 952, 1105, 1182, 1954, 2008
PleI GAGTC 4 cut(s) 842, 905, 967, 1029
PpsI GAGTC 4 cut(s) 842, 905, 967, 1029
Ppu21I YACGTR 1 cut(s) 1009
PsiI TTATAA 1 cut(s) 1805
Psp6I CCWGG 4 cut(s) 520, 1126, 1190, 1227
PspGI CCWGG 4 cut(s) 520, 1126, 1190, 1227
PspN4I GGNNCC 1 cut(s) 1086
PspPI GGNCC 4 cut(s) 186, 367, 496, 1972
PstI CTGCAG 2 cut(s) 226, 823
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 4 cut(s) 101, 1084, 1868, 1921
PvuII CAGCTG 1 cut(s) 950
RruI TCGCGA 1 cut(s) 1473
RsaI GTAC 4 cut(s) 993, 1007, 1967, 2348
RsaNI GTAC 4 cut(s) 992, 1006, 1966, 2347
RseI CAYNNNNRTG 3 cut(s) 287, 480, 1193
SapI GCTCTTC 1 cut(s) 1938
SaqAI TTAA 8 cut(s) 174, 603, 1062, 1430, 1574, 2250, 2319, 2462
SatI GCNGC 9 cut(s) 201, 768, 819, 867, 951, 1104, 1181, 1953, 2007
Sau96I GGNCC 4 cut(s) 186, 367, 496, 1972
SchI GAGTC 4 cut(s) 842, 905, 968, 1029
ScrFI CCNGG 5 cut(s) 522, 1128, 1175, 1192, 1229
SduI GDGCHC 3 cut(s) 55, 622, 2087
SfaNI GCATC 5 cut(s) 144, 496, 694, 853, 1105
SfcI CTRYAG 2 cut(s) 222, 819
SinI GGWCC 3 cut(s) 186, 496, 1972
SmiMI CAYNNNNRTG 3 cut(s) 287, 480, 1193
SmlI CTYRAG 5 cut(s) 669, 1061, 2176, 2318, 2476
SmoI CTYRAG 5 cut(s) 669, 1061, 2176, 2318, 2476
SphI GCATGC 1 cut(s) 2337
SsiI CCGC 9 cut(s) 15, 63, 487, 499, 866, 1014, 1706, 2036, 2262
SspMI CTAG 4 cut(s) 243, 1362, 1919, 2049
StyD4I CCNGG 5 cut(s) 520, 1126, 1173, 1190, 1227
StyI CCWWGG 2 cut(s) 1959, 2436
TaaI ACNGT 8 cut(s) 30, 183, 208, 697, 859, 1649, 1794, 2278
TaiI ACGT 4 cut(s) 655, 993, 1011, 1837
TaqI TCGA 3 cut(s) 846, 962, 1080
TauI GCSGC 1 cut(s) 869
Tru1I TTAA 8 cut(s) 174, 603, 1062, 1430, 1574, 2250, 2319, 2462
Tru9I TTAA 8 cut(s) 174, 603, 1062, 1430, 1574, 2250, 2319, 2462
TscAI CASTG 8 cut(s) 231, 294, 310, 700, 816, 1455, 1464, 2281
TseFI GTSAC 3 cut(s) 149, 377, 1009
TseI GCWGC 8 cut(s) 200, 767, 818, 950, 1103, 1180, 1952, 2006
Tsp45I GTSAC 3 cut(s) 149, 377, 1009
TspGWI ACGGA 3 cut(s) 112, 568, 1163
TspRI CASTG 8 cut(s) 231, 294, 310, 700, 816, 1455, 1464, 2281
Van91I CCANNNNNTGG 1 cut(s) 364
Vha464I CTTAAG 2 cut(s) 1061, 2318
VpaK11BI GGWCC 3 cut(s) 186, 496, 1972
XapI RAATTY 6 cut(s) 674, 830, 1050, 1384, 1890, 2155
XceI RCATGY 3 cut(s) 1905, 2337, 2570
XmnI GAANNNNTTC 1 cut(s) 1782
XspI CTAG 4 cut(s) 243, 1362, 1919, 2049
ZraI GACGTC 1 cut(s) 653
Zsp2I ATGCAT 2 cut(s) 1120, 2339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.