Rh7BG462300

protein At4g37920, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0014976
Physical Location & Seq
Forward (+)
54800630 .. 54804439
3810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG462300.1

Sequence Viewer

Length: 1083 bp
ATGGAGGTTTCTACAGCCACACTCCAACCCTCTTGCTCTTTCCCGCTAAGAGCCCCTAAAACAATTACCAGTGACCGCGGTTCTTCTCTATCTTTCATCAGAACTTCGTCTAAAAACTCCTCCCTATCCTCTAAAACCATCAAAGGTTTTCAGTTACTAGTTAAACCGGTCAGGTTGCGGCGGCTATCGGTTGTTGCAGCTGTTGTTGGTGATAAAACTTCAGTGCCAAGTGATTGTTCTAGTGGGGAAGAGTTGTCTGTTTCTGATTCTTCTGATCCTACTTTGGTTGGAGATGAACATGGAGGAAATGGTGAGAAGGGGGAAGAGGAAGGTGGTGTTGGGTCCTTGGATGACCACAAAATGACCCGAATATGCGATAAGCTTATTGAGGTTTTCATGGTTGACAAGCCCACTCCAACTGACTGGAGAAGATTATTGGCTTTCAGTAGGGAATGGGATGATATCAGGCCTCATTTCTTTAAGCATTGTCAGGTCCGAGCAGATGCTGAGGATGATCCTGGAATGAAGCATAAGATACTTCGGCTTGGGCGGAAATTGAAGGAGATTGATGAAGATGTGCAAAGACACAATGAACTGCTTAAAGTTGTTAGAGGGGCTCCATCAGATATCAGTGACATAGTTGCCAGACGTCGCAAAGATTTCACAAAAGAATTTTTTGTGCATCTTCACACAGTTTCTGAGTCCTATTATGAGAATCTAGAAGAGCAAAATGCTTTGGCAAGCATTGGAAATACGTGCTTGGCAGCTGTACAAGCATATGATTCTGCAACCGAAAGCATTGAAGCACTGAATGCTGCAGAGTTGAAATTTCAAGATATTATCGACTCTCCTTCTGTTGATGCTGCTTGTAGGAAGATAGACCACTTGGCTGAGAAAAATCAACTCGACTCAGCATTGGTTATGATGATTACAAAAGCTTGGTCAGCTGCTAAGGAATCGAGCATGACAAAAGATGAGGTAAATGATGGCTTGCCTGATAAACACTGCTCTTTGTATAGTTGTAAATTTCTAATAAAGTGGTTGCAGCACTATTATGGCAAACCAATGCATTCTTATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

360

Amino Acids

40.12

Weight (kDa)

5.98

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G36320
fragaria_vesca FvH4_5g38330 FvH4_5g38330 FvH4_5g38330
malus_domestica MD08G1174600.v1.1 MD15G1361200.v1.1
prunus_persica Prupe.1G508600_v2.0.a1 Prupe.1G508600_v2.0.a1
pyrus_communis pycom08g14960
rosa_chinensis RchiOBHm_Chr7g0240351
rosa_multiflora Rmu_sc0038179.1_g000001
rosa_roxburghii Rroxscaffold_3G00220200
rosa_samantha Rh7AG489300 Rh7BG462300 Rh7CG505600
rosa_wichuraiana Rw7G040940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 652
AccII CGCG 1 cut(s) 78
AciI CCGC 6 cut(s) 44, 76, 78, 178, 181, 550
AclWI GGATC 2 cut(s) 269, 509
AcsI RAATTY 3 cut(s) 671, 827, 1025
AcuI CTGAAG 1 cut(s) 204
AcyI GRCGYC 1 cut(s) 649
AfaI GTAC 1 cut(s) 771
AgeI ACCGGT 1 cut(s) 166
AgsI TTSAA 4 cut(s) 559, 803, 826, 833
AhlI ACTAGT 1 cut(s) 157
AjnI CCWGG 1 cut(s) 517
AjuI GAANNNNNNNTTGG 4 cut(s) 220, 252, 321, 353
AluBI AGCT 5 cut(s) 200, 382, 767, 938, 947
AluI AGCT 5 cut(s) 200, 382, 767, 938, 947
AlwI GGATC 2 cut(s) 269, 509
AlwNI CAGNNNCTG 2 cut(s) 506, 698
AoxI GGCC 1 cut(s) 467
ApeKI GCWGC 6 cut(s) 197, 764, 815, 863, 947, 1045
ApoI RAATTY 3 cut(s) 671, 827, 1025
ArsI GACNNNNNNTTYG 2 cut(s) 926, 958
AsiGI ACCGGT 1 cut(s) 166
AspS9I GGNCC 2 cut(s) 342, 493
AsuHPI GGTGA 2 cut(s) 221, 323
AvaII GGWCC 2 cut(s) 342, 493
BanII GRGCYC 2 cut(s) 55, 619
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 6 cut(s) 209, 776, 802, 850, 934, 1057
BccI CCATC 3 cut(s) 146, 628, 980
BciT130I CCWGG 1 cut(s) 519
BcuI ACTAGT 1 cut(s) 157
BfaI CTAG 3 cut(s) 158, 240, 719
BfmI CTRYAG 2 cut(s) 12, 816
BisI GCNGC 8 cut(s) 179, 182, 198, 765, 816, 864, 948, 1046
BlsI GCNGC 8 cut(s) 180, 183, 199, 766, 817, 865, 949, 1047
Bme1390I CCNGG 1 cut(s) 519
Bme18I GGWCC 2 cut(s) 342, 493
BmgT120I GGNCC 2 cut(s) 342, 493
BmiI GGNNCC 2 cut(s) 343, 618
BmrFI CCNGG 1 cut(s) 519
BmsI GCATC 3 cut(s) 493, 691, 850
BpmI CTGGAG 1 cut(s) 445
Bpu10I CCTNAGC 2 cut(s) 507, 951
BsaAI YACGTR 1 cut(s) 756
BsaHI GRCGYC 1 cut(s) 649
BsaJI CCNNGG 2 cut(s) 76, 345
BsaWI WCCGGW 1 cut(s) 166
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 2 cut(s) 69, 428
BseBI CCWGG 1 cut(s) 519
BseDI CCNNGG 2 cut(s) 76, 345
BseGI GGATG 3 cut(s) 355, 463, 517
BseMII CTCAG 4 cut(s) 498, 690, 882, 924
BseNI ACTGG 2 cut(s) 69, 428
BseRI GAGGAG 1 cut(s) 109
BseXI GCAGC 6 cut(s) 209, 776, 802, 850, 934, 1057
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 1 cut(s) 469
BshTI ACCGGT 1 cut(s) 166
BsiSI CCGG 1 cut(s) 167
BsmI GAATGC 2 cut(s) 817, 1069
BsnI GGCC 1 cut(s) 469
Bsp1286I GDGCHC 2 cut(s) 55, 619
Bsp1407I TGTACA 1 cut(s) 769
Bsp143I GATC 2 cut(s) 274, 514
BspACI CCGC 6 cut(s) 44, 76, 78, 178, 181, 550
BspANI GGCC 1 cut(s) 469
BspCNI CTCAG 4 cut(s) 499, 691, 883, 923
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 2 cut(s) 343, 618
BspMAI CTGCAG 1 cut(s) 820
BspPI GGATC 2 cut(s) 269, 509
BspQI GCTCTTC 1 cut(s) 717
BsrFI RCCGGY 1 cut(s) 166
BsrGI TGTACA 1 cut(s) 769
BsrI ACTGG 2 cut(s) 69, 428
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 2 cut(s) 76, 345
BssMI GATC 2 cut(s) 274, 514
BssNI GRCGYC 1 cut(s) 649
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 519
Bst4CI ACNGT 1 cut(s) 694
Bst6I CTCTTC 3 cut(s) 243, 318, 717
BstACI GRCGYC 1 cut(s) 649
BstAPI GCANNNNNTGC 1 cut(s) 812
BstAUI TGTACA 1 cut(s) 769
BstBAI YACGTR 1 cut(s) 756
BstC8I GCNNGC 2 cut(s) 742, 992
BstDEI CTNAG 6 cut(s) 47, 507, 699, 891, 910, 951
BstDSI CCRYGG 1 cut(s) 76
BstF5I GGATG 3 cut(s) 355, 463, 517
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 2 cut(s) 277, 517
BstMBI GATC 2 cut(s) 274, 514
BstMWI GCNNNNNNNGC 3 cut(s) 773, 812, 944
BstNI CCWGG 1 cut(s) 519
BstSCI CCNGG 1 cut(s) 517
BstSFI CTRYAG 2 cut(s) 12, 816
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 6 cut(s) 209, 776, 802, 850, 934, 1057
BstXI CCANNNNNNTGG 1 cut(s) 423
BsuRI GGCC 1 cut(s) 469
BtgI CCRYGG 1 cut(s) 76
BtsCI GGATG 3 cut(s) 355, 463, 517
BtsI GCAGTG 1 cut(s) 1003
BtsIMutI CAGTG 5 cut(s) 76, 228, 637, 806, 1003
Cac8I GCNNGC 2 cut(s) 742, 992
CaiI CAGNNNCTG 2 cut(s) 506, 698
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 2 cut(s) 342, 493
Cfr42I CCGCGG 1 cut(s) 79
Csp6I GTAC 1 cut(s) 770
CspAI ACCGGT 1 cut(s) 166
CviAII CATG 3 cut(s) 299, 397, 964
CviQI GTAC 1 cut(s) 770
DdeI CTNAG 6 cut(s) 47, 507, 699, 891, 910, 951
DpnI GATC 2 cut(s) 276, 516
DpnII GATC 2 cut(s) 274, 514
Eam1104I CTCTTC 3 cut(s) 243, 318, 717
EarI CTCTTC 3 cut(s) 243, 318, 717
EciI GGCGGA 1 cut(s) 565
Eco130I CCWWGG 1 cut(s) 345
Eco147I AGGCCT 1 cut(s) 469
Eco24I GRGCYC 2 cut(s) 55, 619
Eco32I GATATC 2 cut(s) 463, 628
Eco47I GGWCC 2 cut(s) 342, 493
Eco57I CTGAAG 1 cut(s) 204
EcoO109I RGGNCCY 1 cut(s) 342
EcoRII CCWGG 1 cut(s) 517
EcoRV GATATC 2 cut(s) 463, 628
EcoT14I CCWWGG 1 cut(s) 345
EcoT22I ATGCAT 2 cut(s) 1071, 1081
EcoT38I GRGCYC 2 cut(s) 55, 619
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 3 cut(s) 302, 400, 967
FatI CATG 3 cut(s) 298, 396, 963
FauI CCCGC 1 cut(s) 51
FauNDI CATATG 1 cut(s) 778
Fnu4HI GCNGC 8 cut(s) 179, 182, 198, 765, 816, 864, 948, 1046
FokI GGATG 3 cut(s) 362, 470, 524
FriOI GRGCYC 2 cut(s) 55, 619
Fsp4HI GCNGC 8 cut(s) 179, 182, 198, 765, 816, 864, 948, 1046
FspBI CTAG 3 cut(s) 158, 240, 719
GluI GCNGC 8 cut(s) 179, 182, 198, 765, 816, 864, 948, 1046
GsuI CTGGAG 1 cut(s) 445
HaeIII GGCC 1 cut(s) 469
HapII CCGG 1 cut(s) 167
Hin1I GRCGYC 1 cut(s) 649
Hin1II CATG 3 cut(s) 302, 400, 967
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HindIII AAGCTT 2 cut(s) 380, 936
HinfI GANTC 7 cut(s) 266, 701, 715, 782, 845, 908, 956
HpaII CCGG 1 cut(s) 167
HphI GGTGA 2 cut(s) 221, 323
Hpy166II GTNNAC 1 cut(s) 403
Hpy188I TCNGA 6 cut(s) 101, 265, 274, 497, 625, 700
Hpy188III TCNNGA 2 cut(s) 719, 833
Hpy8I GTNNAC 1 cut(s) 403
Hpy99I CGWCG 1 cut(s) 654
HpyAV CCTTC 4 cut(s) 310, 323, 553, 861
HpyCH4III ACNGT 1 cut(s) 694
HpyCH4IV ACGT 2 cut(s) 649, 755
HpyCH4V TGCA 8 cut(s) 197, 580, 682, 788, 818, 1045, 1069, 1079
HpyF10VI GCNNNNNNNGC 3 cut(s) 773, 812, 944
HpyF3I CTNAG 6 cut(s) 47, 507, 699, 891, 910, 951
HpySE526I ACGT 2 cut(s) 649, 755
Hsp92I GRCGYC 1 cut(s) 649
Hsp92II CATG 3 cut(s) 302, 400, 967
KspI CCGCGG 1 cut(s) 79
Kzo9I GATC 2 cut(s) 274, 514
LguI GCTCTTC 1 cut(s) 717
LmnI GCTCC 1 cut(s) 622
Lsp1109I GCAGC 6 cut(s) 209, 776, 802, 850, 934, 1057
LweI GCATC 3 cut(s) 493, 691, 850
MaeI CTAG 3 cut(s) 158, 240, 719
MaeII ACGT 2 cut(s) 649, 755
MaeIII GTNAC 3 cut(s) 71, 153, 632
MalI GATC 2 cut(s) 276, 516
MboI GATC 2 cut(s) 274, 514
MboII GAAGA 9 cut(s) 75, 260, 261, 335, 441, 584, 677, 734, 886
MhlI GDGCHC 2 cut(s) 55, 619
MluCI AATT 5 cut(s) 63, 554, 671, 827, 1025
MlyI GAGTC 3 cut(s) 710, 839, 902
MmeI TCCRAC 3 cut(s) 49, 268, 440
Mph1103I ATGCAT 2 cut(s) 1071, 1081
MseI TTAA 3 cut(s) 162, 480, 600
MslI CAYNNNNRTG 2 cut(s) 1053, 1074
MspA1I CMGCKG 4 cut(s) 78, 200, 767, 947
MspI CCGG 1 cut(s) 167
MspR9I CCNGG 1 cut(s) 519
Mva1269I GAATGC 2 cut(s) 817, 1069
MvaI CCWGG 1 cut(s) 519
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 3 cut(s) 773, 812, 944
NdeI CATATG 1 cut(s) 778
NdeII GATC 2 cut(s) 274, 514
NlaIII CATG 3 cut(s) 302, 400, 967
NlaIV GGNNCC 2 cut(s) 343, 618
NmuCI GTSAC 2 cut(s) 71, 632
NsiI ATGCAT 2 cut(s) 1071, 1081
PceI AGGCCT 1 cut(s) 469
PciSI GCTCTTC 1 cut(s) 717
PctI GAATGC 2 cut(s) 817, 1069
PfeI GAWTC 4 cut(s) 266, 715, 782, 956
PfoI TCCNGGA 1 cut(s) 517
PinAI ACCGGT 1 cut(s) 166
PkrI GCNGC 8 cut(s) 180, 183, 199, 766, 817, 865, 949, 1047
PleI GAGTC 3 cut(s) 709, 839, 902
PpsI GAGTC 3 cut(s) 709, 839, 902
Ppu21I YACGTR 1 cut(s) 756
PpuMI RGGWCCY 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 517
PspGI CCWGG 1 cut(s) 517
PspN4I GGNNCC 2 cut(s) 343, 618
PspPI GGNCC 2 cut(s) 342, 493
PspPPI RGGWCCY 1 cut(s) 342
PstI CTGCAG 1 cut(s) 820
PstNI CAGNNNCTG 2 cut(s) 506, 698
PvuII CAGCTG 3 cut(s) 200, 767, 947
RsaI GTAC 1 cut(s) 771
RsaNI GTAC 1 cut(s) 770
RseI CAYNNNNRTG 2 cut(s) 1053, 1074
SacII CCGCGG 1 cut(s) 79
SapI GCTCTTC 1 cut(s) 717
SaqAI TTAA 3 cut(s) 162, 480, 600
SatI GCNGC 8 cut(s) 179, 182, 198, 765, 816, 864, 948, 1046
Sau3AI GATC 2 cut(s) 274, 514
Sau96I GGNCC 2 cut(s) 342, 493
SchI GAGTC 3 cut(s) 710, 839, 902
ScrFI CCNGG 1 cut(s) 519
SduI GDGCHC 2 cut(s) 55, 619
SfaNI GCATC 3 cut(s) 493, 691, 850
SfcI CTRYAG 2 cut(s) 12, 816
Sfr303I CCGCGG 1 cut(s) 79
SgrBI CCGCGG 1 cut(s) 79
SinI GGWCC 2 cut(s) 342, 493
SmiMI CAYNNNNRTG 2 cut(s) 1053, 1074
SpeI ACTAGT 1 cut(s) 157
Sse9I AATT 5 cut(s) 63, 554, 671, 827, 1025
SseBI AGGCCT 1 cut(s) 469
SsiI CCGC 6 cut(s) 44, 76, 78, 178, 181, 550
SspMI CTAG 3 cut(s) 158, 240, 719
StuI AGGCCT 1 cut(s) 469
StyD4I CCNGG 1 cut(s) 517
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 1 cut(s) 694
TaiI ACGT 2 cut(s) 652, 758
TaqI TCGA 3 cut(s) 843, 906, 959
TasI AATT 5 cut(s) 63, 554, 671, 827, 1025
TatI WGTACW 1 cut(s) 769
TauI GCSGC 2 cut(s) 181, 184
TfiI GAWTC 4 cut(s) 266, 715, 782, 956
Tru1I TTAA 3 cut(s) 162, 480, 600
Tru9I TTAA 3 cut(s) 162, 480, 600
TscAI CASTG 5 cut(s) 76, 228, 637, 813, 1010
TseFI GTSAC 2 cut(s) 71, 632
TseI GCWGC 6 cut(s) 197, 764, 815, 863, 947, 1045
Tsp45I GTSAC 2 cut(s) 71, 632
TspDTI ATGAA 6 cut(s) 85, 309, 385, 539, 585, 606
TspRI CASTG 5 cut(s) 76, 228, 637, 813, 1010
VpaK11BI GGWCC 2 cut(s) 342, 493
XapI RAATTY 3 cut(s) 671, 827, 1025
XbaI TCTAGA 1 cut(s) 718
XspI CTAG 3 cut(s) 158, 240, 719
ZraI GACGTC 1 cut(s) 650
Zsp2I ATGCAT 2 cut(s) 1071, 1081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.