Rw7G040940

protein At4g37920, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014976
Physical Location & Seq
Forward (+)
63887644 .. 63891523
3880 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G040940.1

Sequence Viewer

Length: 1326 bp
ATGGAGGTTTCTACAGCCACTCTCCAACCCTCTTGTTCTTTCCCTCTAAGAGCCCCTAAAACAATTACCAGTGACCGCGGTTCTTCTCTATCTTTCATCAGAACTTCGTCTGAAAACTTCTCCCTATCCTCTAAACCCATCAAAGGTTTTCAGTTACTAGTTAAACCGGTCAGGTTGCGGCGGCTATCGGTTGTTGCAGCTGTTGTTGGTGATAAAGCTTCAGTGTCAAGTGATTGTTCTAGTGGGGAAGAGTTGTCTGTTTCTGATTCTTCTGATCCTACTTTGGTTGGAGATGAACATGGAGGAAATGGTGAGAAGGGGGAAGAGGAAGGTGGTGTTGGGTCCTTGGATGACCACAAAATGACCAGAATATGCGATAAGCTTATTGAGGTTTTCATGGTTGACAAGCCCACTCCAACTGACTGGAGAAGATTATTGGCTTTCAGTAGGGAATGGGATGATATCAGGCCTCATTTCTTTAAGCGTTGTCAGGTCCGAGCAGATGCTGAGGATGATCCTGGAATGAAGCATAAGATACTTTGGCTTGGGCGGAAATTGAAAGAGATTGATGAAGATGTTCAAAGACACAATGAACTGCTTAAAGTTGTTAGAGGGGCTCCATCAGATATTAGTGACATAGTTGCCAGACGTCGCAAAGATTTCACAAAAGAATTTTTTGTGCATCTTCACACGGTTTCTGAGTCCTATTATGAGAATCTAGAAGAGCAAAATGCTTTGGCAAGCATTGGAAATACATGCTTGGCAGCTGTACAAGCATATGATTCTGCAACTGAAAGCATTGAAGCACTGAATGCTGCAGAGTTGAAATTTCAAGATATTATCGATTCTCCTTCTGTTGATGCTGCTTGTAGGAAGATAGACCACTTGGCTGAGAAAAATCAACTCGACTCAGCATTGGTTATGATGATTACAAAAGCTTGGTCAGCTGCTAAAGAATCGAGCATGACGAAAGATGAGGTAAAAGATGTATTATATCACTTGTATGTAACTGCAAGAGGTAATCTTCAGAAGCTCATGCCAAAAGAAATTCGGATAGTTAAGTATCTGCTCACAATTGACGATCCTGAGGAGCGGCTGTCTGCCTTACAAGATGCATTCACCCCGGGAGAGGAACTTGAAGGGAAGGATGTAGATAACCTCTACACGACCCCGGAGAAGCTTCACACCTGGATAAAGGCTGTCGTGGATGCTTATCATTTGAGCAGGGAAGGTACTCTAATAAGGGAAGCAAGGGACCTGATGAATCCAAAGATCATAAAAAAACTGGAGGACCTGAAGAAGGTAGTTGAAAACAAGTTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

441

Amino Acids

49.54

Weight (kDa)

5.54

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G36320
fragaria_vesca FvH4_5g38330 FvH4_5g38330 FvH4_5g38330
malus_domestica MD08G1174600.v1.1 MD15G1361200.v1.1
prunus_persica Prupe.1G508600_v2.0.a1 Prupe.1G508600_v2.0.a1
pyrus_communis pycom08g14960
rosa_chinensis RchiOBHm_Chr7g0240351
rosa_multiflora Rmu_sc0038179.1_g000001
rosa_roxburghii Rroxscaffold_3G00220200
rosa_samantha Rh7AG489300 Rh7BG462300 Rh7CG505600
rosa_wichuraiana Rw7G040940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 652
AccBSI CCGCTC 1 cut(s) 1093
AccII CGCG 1 cut(s) 78
AciI CCGC 6 cut(s) 76, 78, 178, 181, 550, 1093
AclWI GGATC 3 cut(s) 269, 509, 1076
AcsI RAATTY 3 cut(s) 671, 827, 1047
AcuI CTGAAG 3 cut(s) 204, 1010, 1316
AcyI GRCGYC 1 cut(s) 649
AfaI GTAC 2 cut(s) 771, 1234
AfiI CCNNNNNNNGG 3 cut(s) 143, 1129, 1300
AgeI ACCGGT 1 cut(s) 166
AgsI TTSAA 7 cut(s) 559, 581, 803, 826, 833, 1139, 1310
AhlI ACTAGT 1 cut(s) 157
AjnI CCWGG 2 cut(s) 517, 1187
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AluBI AGCT 8 cut(s) 200, 218, 382, 767, 938, 947, 1033, 1180
AluI AGCT 8 cut(s) 200, 218, 382, 767, 938, 947, 1033, 1180
AlwI GGATC 3 cut(s) 269, 509, 1076
AlwNI CAGNNNCTG 1 cut(s) 506
Ama87I CYCGRG 1 cut(s) 1123
AoxI GGCC 1 cut(s) 467
ApeKI GCWGC 5 cut(s) 197, 764, 815, 863, 947
ApoI RAATTY 3 cut(s) 671, 827, 1047
ArsI GACNNNNNNTTYG 2 cut(s) 926, 958
AsiGI ACCGGT 1 cut(s) 166
Asp700I GAANNNNTTC 1 cut(s) 576
AspS9I GGNCC 4 cut(s) 342, 493, 1255, 1291
AsuC2I CCSGG 3 cut(s) 1124, 1125, 1172
AsuHPI GGTGA 3 cut(s) 221, 323, 1111
AvaI CYCGRG 1 cut(s) 1123
AvaII GGWCC 4 cut(s) 342, 493, 1255, 1291
AxyI CCTNAGG 1 cut(s) 1086
BanII GRGCYC 2 cut(s) 55, 619
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 5 cut(s) 209, 776, 802, 850, 934
BccI CCATC 2 cut(s) 146, 628
BciT130I CCWGG 2 cut(s) 519, 1189
BcnI CCSGG 3 cut(s) 1124, 1125, 1172
BcuI ACTAGT 1 cut(s) 157
BfaI CTAG 3 cut(s) 158, 240, 719
BfmI CTRYAG 2 cut(s) 12, 816
BisI GCNGC 8 cut(s) 179, 182, 198, 765, 816, 864, 948, 1094
BlsI GCNGC 8 cut(s) 180, 183, 199, 766, 817, 865, 949, 1095
Bme1390I CCNGG 5 cut(s) 519, 1124, 1125, 1172, 1189
Bme18I GGWCC 4 cut(s) 342, 493, 1255, 1291
BmeT110I CYCGRG 1 cut(s) 1123
BmgT120I GGNCC 4 cut(s) 342, 493, 1255, 1291
BmiI GGNNCC 3 cut(s) 343, 618, 1256
BmrFI CCNGG 5 cut(s) 519, 1124, 1125, 1172, 1189
BmsI GCATC 5 cut(s) 493, 691, 850, 1102, 1198
BpmI CTGGAG 2 cut(s) 445, 1307
Bpu10I CCTNAGC 1 cut(s) 507
BpuMI CCSGG 3 cut(s) 1124, 1125, 1172
Bsa29I ATCGAT 1 cut(s) 843
BsaBI GATNNNNATC 1 cut(s) 1212
BsaHI GRCGYC 1 cut(s) 649
BsaJI CCNNGG 5 cut(s) 76, 345, 1122, 1123, 1170
BsaWI WCCGGW 1 cut(s) 166
BsaXI ACNNNNNCTCC 2 cut(s) 1082, 1112
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 1129, 1300
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 3 cut(s) 69, 428, 1290
Bse21I CCTNAGG 1 cut(s) 1086
Bse8I GATNNNNATC 1 cut(s) 1212
BseBI CCWGG 2 cut(s) 519, 1189
BseCI ATCGAT 1 cut(s) 843
BseDI CCNNGG 5 cut(s) 76, 345, 1122, 1123, 1170
BseGI GGATG 5 cut(s) 355, 463, 517, 1153, 1213
BseJI GATNNNNATC 1 cut(s) 1212
BseLI CCNNNNNNNGG 3 cut(s) 143, 1129, 1300
BseMII CTCAG 5 cut(s) 498, 690, 882, 924, 1077
BseNI ACTGG 3 cut(s) 69, 428, 1290
BseRI GAGGAG 1 cut(s) 1103
BseXI GCAGC 5 cut(s) 209, 776, 802, 850, 934
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 1 cut(s) 469
BshTI ACCGGT 1 cut(s) 166
BshVI ATCGAT 1 cut(s) 843
BsiHKCI CYCGRG 1 cut(s) 1123
BsiSI CCGG 3 cut(s) 167, 1124, 1172
BslFI GGGAC 1 cut(s) 1268
BslI CCNNNNNNNGG 3 cut(s) 143, 1129, 1300
BsmFI GGGAC 1 cut(s) 1268
BsmI GAATGC 2 cut(s) 817, 1115
BsnI GGCC 1 cut(s) 469
BsoBI CYCGRG 1 cut(s) 1123
Bsp1286I GDGCHC 2 cut(s) 55, 619
Bsp1407I TGTACA 1 cut(s) 769
Bsp143I GATC 4 cut(s) 274, 514, 1081, 1272
BspACI CCGC 6 cut(s) 76, 78, 178, 181, 550, 1093
BspANI GGCC 1 cut(s) 469
BspCNI CTCAG 5 cut(s) 499, 691, 883, 923, 1078
BspDI ATCGAT 1 cut(s) 843
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 3 cut(s) 343, 618, 1256
BspMAI CTGCAG 1 cut(s) 820
BspPI GGATC 3 cut(s) 269, 509, 1076
BspQI GCTCTTC 1 cut(s) 717
BsrBI CCGCTC 1 cut(s) 1093
BsrFI RCCGGY 1 cut(s) 166
BsrGI TGTACA 1 cut(s) 769
BsrI ACTGG 3 cut(s) 69, 428, 1290
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 5 cut(s) 76, 345, 1122, 1123, 1170
BssMI GATC 4 cut(s) 274, 514, 1081, 1272
BssNI GRCGYC 1 cut(s) 649
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 2 cut(s) 519, 1189
Bst4CI ACNGT 1 cut(s) 694
Bst6I CTCTTC 3 cut(s) 243, 318, 717
BstACI GRCGYC 1 cut(s) 649
BstAPI GCANNNNNTGC 1 cut(s) 812
BstAUI TGTACA 1 cut(s) 769
BstC8I GCNNGC 1 cut(s) 742
BstDEI CTNAG 6 cut(s) 47, 507, 699, 891, 910, 1086
BstDSI CCRYGG 1 cut(s) 76
BstENI CCTNNNNNAGG 1 cut(s) 1298
BstF5I GGATG 5 cut(s) 355, 463, 517, 1153, 1213
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 4 cut(s) 277, 517, 1084, 1275
BstMBI GATC 4 cut(s) 274, 514, 1081, 1272
BstMWI GCNNNNNNNGC 3 cut(s) 773, 812, 944
BstNI CCWGG 2 cut(s) 519, 1189
BstNSI RCATGY 1 cut(s) 759
BstSCI CCNGG 5 cut(s) 517, 1122, 1123, 1170, 1187
BstSFI CTRYAG 2 cut(s) 12, 816
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 5 cut(s) 209, 776, 802, 850, 934
BstXI CCANNNNNNTGG 1 cut(s) 423
Bsu15I ATCGAT 1 cut(s) 843
Bsu36I CCTNAGG 1 cut(s) 1086
BsuRI GGCC 1 cut(s) 469
BsuTUI ATCGAT 1 cut(s) 843
BtgI CCRYGG 1 cut(s) 76
BtsCI GGATG 5 cut(s) 355, 463, 517, 1153, 1213
BtsIMutI CAGTG 3 cut(s) 76, 228, 806
Cac8I GCNNGC 1 cut(s) 742
CaiI CAGNNNCTG 1 cut(s) 506
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 4 cut(s) 342, 493, 1255, 1291
Cfr42I CCGCGG 1 cut(s) 79
Cfr9I CCCGGG 1 cut(s) 1123
ClaI ATCGAT 1 cut(s) 843
Csp6I GTAC 2 cut(s) 770, 1233
CspAI ACCGGT 1 cut(s) 166
CviAII CATG 5 cut(s) 299, 397, 756, 964, 1036
CviQI GTAC 2 cut(s) 770, 1233
DdeI CTNAG 6 cut(s) 47, 507, 699, 891, 910, 1086
DpnI GATC 4 cut(s) 276, 516, 1083, 1274
DpnII GATC 4 cut(s) 274, 514, 1081, 1272
Eam1104I CTCTTC 3 cut(s) 243, 318, 717
EarI CTCTTC 3 cut(s) 243, 318, 717
EciI GGCGGA 1 cut(s) 565
Eco130I CCWWGG 1 cut(s) 345
Eco147I AGGCCT 1 cut(s) 469
Eco24I GRGCYC 2 cut(s) 55, 619
Eco32I GATATC 1 cut(s) 463
Eco47I GGWCC 4 cut(s) 342, 493, 1255, 1291
Eco57I CTGAAG 3 cut(s) 204, 1010, 1316
Eco81I CCTNAGG 1 cut(s) 1086
Eco88I CYCGRG 1 cut(s) 1123
EcoNI CCTNNNNNAGG 1 cut(s) 1298
EcoO109I RGGNCCY 3 cut(s) 342, 1255, 1291
EcoRII CCWGG 2 cut(s) 517, 1187
EcoRV GATATC 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 345
EcoT22I ATGCAT 1 cut(s) 1117
EcoT38I GRGCYC 2 cut(s) 55, 619
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 5 cut(s) 302, 400, 759, 967, 1039
FaqI GGGAC 1 cut(s) 1268
FatI CATG 5 cut(s) 298, 396, 755, 963, 1035
FauNDI CATATG 1 cut(s) 778
Fnu4HI GCNGC 8 cut(s) 179, 182, 198, 765, 816, 864, 948, 1094
FokI GGATG 5 cut(s) 362, 470, 524, 1160, 1220
FriOI GRGCYC 2 cut(s) 55, 619
Fsp4HI GCNGC 8 cut(s) 179, 182, 198, 765, 816, 864, 948, 1094
FspBI CTAG 3 cut(s) 158, 240, 719
GluI GCNGC 8 cut(s) 179, 182, 198, 765, 816, 864, 948, 1094
GsuI CTGGAG 2 cut(s) 445, 1307
HaeIII GGCC 1 cut(s) 469
HapII CCGG 3 cut(s) 167, 1124, 1172
Hin1I GRCGYC 1 cut(s) 649
Hin1II CATG 5 cut(s) 302, 400, 759, 967, 1039
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HindIII AAGCTT 4 cut(s) 216, 380, 936, 1178
HinfI GANTC 8 cut(s) 266, 701, 715, 782, 845, 908, 956, 1264
HpaII CCGG 3 cut(s) 167, 1124, 1172
HphI GGTGA 3 cut(s) 221, 323, 1111
Hpy166II GTNNAC 1 cut(s) 403
Hpy188I TCNGA 9 cut(s) 101, 112, 265, 274, 497, 625, 700, 1029, 1053
Hpy188III TCNNGA 3 cut(s) 719, 833, 1085
Hpy8I GTNNAC 1 cut(s) 403
Hpy99I CGWCG 1 cut(s) 654
HpyAV CCTTC 7 cut(s) 310, 323, 861, 1133, 1138, 1223, 1294
HpyCH4III ACNGT 1 cut(s) 694
HpyCH4IV ACGT 1 cut(s) 649
HpyCH4V TGCA 6 cut(s) 197, 682, 788, 818, 1013, 1115
HpyF10VI GCNNNNNNNGC 3 cut(s) 773, 812, 944
HpyF3I CTNAG 6 cut(s) 47, 507, 699, 891, 910, 1086
HpySE526I ACGT 1 cut(s) 649
Hsp92I GRCGYC 1 cut(s) 649
Hsp92II CATG 5 cut(s) 302, 400, 759, 967, 1039
KspI CCGCGG 1 cut(s) 79
Kzo9I GATC 4 cut(s) 274, 514, 1081, 1272
LguI GCTCTTC 1 cut(s) 717
LmnI GCTCC 2 cut(s) 622, 1090
Lsp1109I GCAGC 5 cut(s) 209, 776, 802, 850, 934
LweI GCATC 5 cut(s) 493, 691, 850, 1102, 1198
MaeI CTAG 3 cut(s) 158, 240, 719
MaeII ACGT 1 cut(s) 649
MaeIII GTNAC 4 cut(s) 71, 153, 632, 1006
MalI GATC 4 cut(s) 276, 516, 1083, 1274
MbiI CCGCTC 1 cut(s) 1093
MboI GATC 4 cut(s) 274, 514, 1081, 1272
MfeI CAATTG 1 cut(s) 1074
MhlI GDGCHC 2 cut(s) 55, 619
MluCI AATT 6 cut(s) 63, 554, 671, 827, 1047, 1074
MlyI GAGTC 2 cut(s) 710, 902
MmeI TCCRAC 3 cut(s) 49, 268, 440
Mph1103I ATGCAT 1 cut(s) 1117
MroXI GAANNNNTTC 1 cut(s) 576
MseI TTAA 4 cut(s) 162, 480, 600, 1059
MslI CAYNNNNRTG 1 cut(s) 1002
MspA1I CMGCKG 4 cut(s) 78, 200, 767, 947
MspI CCGG 3 cut(s) 167, 1124, 1172
MspR9I CCNGG 5 cut(s) 519, 1124, 1125, 1172, 1189
MunI CAATTG 1 cut(s) 1074
Mva1269I GAATGC 2 cut(s) 817, 1115
MvaI CCWGG 2 cut(s) 519, 1189
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 3 cut(s) 773, 812, 944
NciI CCSGG 3 cut(s) 1124, 1125, 1172
NdeI CATATG 1 cut(s) 778
NdeII GATC 4 cut(s) 274, 514, 1081, 1272
NlaIII CATG 5 cut(s) 302, 400, 759, 967, 1039
NlaIV GGNNCC 3 cut(s) 343, 618, 1256
NmuCI GTSAC 2 cut(s) 71, 632
NsiI ATGCAT 1 cut(s) 1117
NspI RCATGY 1 cut(s) 759
PceI AGGCCT 1 cut(s) 469
PciSI GCTCTTC 1 cut(s) 717
PcsI WCGNNNNNNNCGW 1 cut(s) 965
PctI GAATGC 2 cut(s) 817, 1115
PdmI GAANNNNTTC 1 cut(s) 576
PfeI GAWTC 6 cut(s) 266, 715, 782, 845, 956, 1264
PfoI TCCNGGA 1 cut(s) 517
PinAI ACCGGT 1 cut(s) 166
PkrI GCNGC 8 cut(s) 180, 183, 199, 766, 817, 865, 949, 1095
PleI GAGTC 2 cut(s) 709, 902
PpsI GAGTC 2 cut(s) 709, 902
PpuMI RGGWCCY 3 cut(s) 342, 1255, 1291
Psp5II RGGWCCY 3 cut(s) 342, 1255, 1291
Psp6I CCWGG 2 cut(s) 517, 1187
PspGI CCWGG 2 cut(s) 517, 1187
PspN4I GGNNCC 3 cut(s) 343, 618, 1256
PspPI GGNCC 4 cut(s) 342, 493, 1255, 1291
PspPPI RGGWCCY 3 cut(s) 342, 1255, 1291
PstI CTGCAG 1 cut(s) 820
PstNI CAGNNNCTG 1 cut(s) 506
PvuII CAGCTG 3 cut(s) 200, 767, 947
RsaI GTAC 2 cut(s) 771, 1234
RsaNI GTAC 2 cut(s) 770, 1233
RseI CAYNNNNRTG 1 cut(s) 1002
SacII CCGCGG 1 cut(s) 79
SapI GCTCTTC 1 cut(s) 717
SaqAI TTAA 4 cut(s) 162, 480, 600, 1059
SatI GCNGC 8 cut(s) 179, 182, 198, 765, 816, 864, 948, 1094
Sau3AI GATC 4 cut(s) 274, 514, 1081, 1272
Sau96I GGNCC 4 cut(s) 342, 493, 1255, 1291
SchI GAGTC 2 cut(s) 710, 902
ScrFI CCNGG 5 cut(s) 519, 1124, 1125, 1172, 1189
SduI GDGCHC 2 cut(s) 55, 619
SfaNI GCATC 5 cut(s) 493, 691, 850, 1102, 1198
SfcI CTRYAG 2 cut(s) 12, 816
Sfr303I CCGCGG 1 cut(s) 79
SgrBI CCGCGG 1 cut(s) 79
SinI GGWCC 4 cut(s) 342, 493, 1255, 1291
SmaI CCCGGG 1 cut(s) 1125
SmiMI CAYNNNNRTG 1 cut(s) 1002
SpeI ACTAGT 1 cut(s) 157
Sse9I AATT 6 cut(s) 63, 554, 671, 827, 1047, 1074
SseBI AGGCCT 1 cut(s) 469
SsiI CCGC 6 cut(s) 76, 78, 178, 181, 550, 1093
SspMI CTAG 3 cut(s) 158, 240, 719
StuI AGGCCT 1 cut(s) 469
StyD4I CCNGG 5 cut(s) 517, 1122, 1123, 1170, 1187
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 1 cut(s) 694
TaiI ACGT 1 cut(s) 652
TaqI TCGA 3 cut(s) 843, 906, 959
TasI AATT 6 cut(s) 63, 554, 671, 827, 1047, 1074
TatI WGTACW 1 cut(s) 769
TauI GCSGC 3 cut(s) 181, 184, 1096
TfiI GAWTC 6 cut(s) 266, 715, 782, 845, 956, 1264
Tru1I TTAA 4 cut(s) 162, 480, 600, 1059
Tru9I TTAA 4 cut(s) 162, 480, 600, 1059
TscAI CASTG 3 cut(s) 76, 228, 813
TseFI GTSAC 2 cut(s) 71, 632
TseI GCWGC 5 cut(s) 197, 764, 815, 863, 947
Tsp45I GTSAC 2 cut(s) 71, 632
TspDTI ATGAA 7 cut(s) 85, 309, 385, 539, 585, 606, 1277
TspMI CCCGGG 1 cut(s) 1123
TspRI CASTG 3 cut(s) 76, 228, 813
VpaK11BI GGWCC 4 cut(s) 342, 493, 1255, 1291
XagI CCTNNNNNAGG 1 cut(s) 1298
XapI RAATTY 3 cut(s) 671, 827, 1047
XbaI TCTAGA 1 cut(s) 718
XceI RCATGY 1 cut(s) 759
XmaI CCCGGG 1 cut(s) 1123
XmnI GAANNNNTTC 1 cut(s) 576
XspI CTAG 3 cut(s) 158, 240, 719
ZraI GACGTC 1 cut(s) 650
Zsp2I ATGCAT 1 cut(s) 1117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.