pycom08g17500

Sas10/Utp3/C1D family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008750
Physical Location & Seq
Forward (+)
17705686 .. 17708494
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1005 bp
ATGGAAGACGCTTCAAATTCAAACCCAATCTCTGAAAATAGAGTAAAGCAAGAAGCTCCCCAGCTTGCTGCTTTGTTGAGAGAAATGAAGGAAGGATTAGATACAGTGAGGGCAAAAGTCCAAGCTTTAACTGCAAAGGTGAAAGCAAATAATTATCCGACAGCAGACGGGATAAGCTATCTTGAAGCCAAGCATTTGCTGCTTCTAAACTATTGCCAGTCACTTGTCTATTACTTACTTCGTAAGGCTAAAGGGTTTTCAATAAATGGGCACCCCGTTGTTCGTAGCCTTGTGGAGATAAGGTTGTTTCTGGAAAAGATTCGACCCATTGACAAGAAGCTCCAGTACCAAGTTGAGAAGCTCACCAAGGTTACTACGAGCACAACCGAAGATGTAGGGCCAAGTGGGAAGGAACCACAACCCAATGTGCCTCAGCAAACAGATGATTTGCTGAGATACCGCCCTAACCCTGACATGCTTGTTAGCAAAACAGATGTGAATTCTAAGGATGGTAAGGATGTGTATCGACCTCCCAAATTTGCCCCGACTTCGATGGAGGAAGATAAGTTGTCAAAGCAGGAAAAAAATGCATTGAGAAGGGATAAGAATACTTTACGACAAGCTAAGCAGAGTGCTTATGTGAGGGAACTGGTGGATGATTTGGAGGGGAGGCCTGAAGAGATTGTAGAACGTGTTGGAGCTGAAAGCATAGAACTAGCGAGATACAGGGCAAAGAGGGATGAACGTGAACGGCAAGAAGAGGAACTTTTCATGCGTGCTCCTATTACAAAGAAGGAGAAACAGAGAGAAAAACACTTAAAGAAATCAAGAAATGGGTTGCTTGGTTTGACGGAGAATTTCTATGATGAAATTAAGGGTTTACCCTTGGACGATGAGAATGATGAGCAGGTTGGAAGCTCCCGCAGTGCTAGGGGTGGAATGGGGAGGCATAAGAAGCGCAAGGTATGTCATAATCTCTTAAAAAGGAATCAAATGAGGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sas10_Utp3

Protein Analysis

335

Amino Acids

38.45

Weight (kDa)

9.52

Isoelectric Point (pI)

47.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sas10_Utp3 PF04000 26 - 106 2.7e-19 Sas10/Utp3/C1D family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 898
AccB1I GGYRCC 1 cut(s) 270
AciI CCGC 2 cut(s) 460, 922
AcsI RAATTY 4 cut(s) 16, 499, 536, 856
AcuI CTGAAG 1 cut(s) 696
AfaI GTAC 1 cut(s) 347
AflIII ACRYGT 1 cut(s) 691
AgsI TTSAA 4 cut(s) 15, 21, 185, 261
AluBI AGCT 9 cut(s) 56, 64, 125, 177, 340, 361, 623, 701, 918
AluI AGCT 9 cut(s) 56, 64, 125, 177, 340, 361, 623, 701, 918
Alw21I GWGCWC 2 cut(s) 383, 781
AoxI GGCC 2 cut(s) 398, 671
ApeKI GCWGC 2 cut(s) 68, 199
ApoI RAATTY 4 cut(s) 16, 499, 536, 856
Asp700I GAANNNNTTC 1 cut(s) 318
AspLEI GCGC 1 cut(s) 960
AspS9I GGNCC 1 cut(s) 398
AsuHPI GGTGA 2 cut(s) 151, 355
BaeGI GKGCMC 1 cut(s) 273
BanI GGYRCC 1 cut(s) 270
BarI GAAGNNNNNNTAC 2 cut(s) 329, 361
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 2 cut(s) 383, 781
BbvCI CCTCAGC 1 cut(s) 432
BbvI GCAGC 2 cut(s) 55, 186
BccI CCATC 2 cut(s) 503, 547
BceAI ACGGC 1 cut(s) 767
BfaI CTAG 2 cut(s) 716, 930
BfuAI ACCTGC 1 cut(s) 898
BisI GCNGC 2 cut(s) 69, 200
BlpI GCTNAGC 1 cut(s) 624
BlsI GCNGC 2 cut(s) 70, 201
BmgT120I GGNCC 1 cut(s) 398
BmiI GGNNCC 2 cut(s) 272, 414
BpiI GAAGAC 1 cut(s) 12
BpmI CTGGAG 1 cut(s) 326
Bpu10I CCTNAGC 1 cut(s) 432
Bpu1102I GCTNAGC 1 cut(s) 624
BsaBI GATNNNNATC 1 cut(s) 522
BsaJI CCNNGG 2 cut(s) 366, 885
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bse1I ACTGG 3 cut(s) 217, 343, 654
Bse8I GATNNNNATC 1 cut(s) 522
BseDI CCNNGG 2 cut(s) 366, 885
BseGI GGATG 4 cut(s) 514, 523, 661, 745
BseJI GATNNNNATC 1 cut(s) 522
BseMII CTCAG 2 cut(s) 443, 446
BseNI ACTGG 3 cut(s) 217, 343, 654
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 2 cut(s) 55, 186
BseYI CCCAGC 1 cut(s) 60
BshFI GGCC 2 cut(s) 400, 673
BshNI GGYRCC 1 cut(s) 270
BsiHKAI GWGCWC 2 cut(s) 383, 781
BsnI GGCC 2 cut(s) 400, 673
Bsp1286I GDGCHC 3 cut(s) 273, 383, 781
Bsp1720I GCTNAGC 1 cut(s) 624
BspACI CCGC 2 cut(s) 460, 922
BspANI GGCC 2 cut(s) 400, 673
BspCNI CTCAG 2 cut(s) 444, 445
BspLI GGNNCC 2 cut(s) 272, 414
BspMI ACCTGC 1 cut(s) 898
BspT107I GGYRCC 1 cut(s) 270
BsrI ACTGG 3 cut(s) 217, 343, 654
BssECI CCNNGG 2 cut(s) 366, 885
BssT1I CCWWGG 2 cut(s) 366, 885
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 2 cut(s) 672, 753
BstAPI GCANNNNNTGC 1 cut(s) 199
BstC8I GCNNGC 2 cut(s) 66, 777
BstDEI CTNAG 4 cut(s) 432, 452, 504, 624
BstF5I GGATG 4 cut(s) 514, 523, 661, 745
BstHHI GCGC 1 cut(s) 960
BstMWI GCNNNNNNNGC 3 cut(s) 131, 199, 955
BstNSI RCATGY 1 cut(s) 478
BstSLI GKGCMC 1 cut(s) 273
BstV1I GCAGC 2 cut(s) 55, 186
BstV2I GAAGAC 1 cut(s) 12
BsuRI GGCC 2 cut(s) 400, 673
BtsCI GGATG 4 cut(s) 514, 523, 661, 745
BtsI GCAGTG 1 cut(s) 931
BtsIMutI CAGTG 2 cut(s) 111, 931
BveI ACCTGC 1 cut(s) 898
Cac8I GCNNGC 2 cut(s) 66, 777
CfoI GCGC 1 cut(s) 960
Cfr13I GGNCC 1 cut(s) 398
CseI GACGC 1 cut(s) 17
Csp6I GTAC 1 cut(s) 346
CviAII CATG 2 cut(s) 475, 772
CviQI GTAC 1 cut(s) 346
DdeI CTNAG 4 cut(s) 432, 452, 504, 624
Eam1104I CTCTTC 2 cut(s) 672, 753
EarI CTCTTC 2 cut(s) 672, 753
Eco130I CCWWGG 2 cut(s) 366, 885
Eco147I AGGCCT 1 cut(s) 673
Eco57I CTGAAG 1 cut(s) 696
EcoRI GAATTC 1 cut(s) 499
EcoT14I CCWWGG 2 cut(s) 366, 885
EcoT22I ATGCAT 1 cut(s) 592
ErhI CCWWGG 2 cut(s) 366, 885
FaeI CATG 2 cut(s) 478, 775
FaiI YATR 8 cut(s) 476, 639, 710, 773, 864, 951, 967, 972
FalI AAGNNNNNCTT 2 cut(s) 750, 782
FatI CATG 2 cut(s) 474, 771
FauI CCCGC 1 cut(s) 929
Fnu4HI GCNGC 2 cut(s) 69, 200
FokI GGATG 4 cut(s) 521, 530, 668, 752
Fsp4HI GCNGC 2 cut(s) 69, 200
FspBI CTAG 2 cut(s) 716, 930
GlaI GCGC 1 cut(s) 959
GluI GCNGC 2 cut(s) 69, 200
GsaI CCCAGC 1 cut(s) 64
GsuI CTGGAG 1 cut(s) 326
HaeIII GGCC 2 cut(s) 400, 673
HgaI GACGC 1 cut(s) 17
HhaI GCGC 1 cut(s) 960
Hin1II CATG 2 cut(s) 478, 775
Hin6I GCGC 1 cut(s) 958
HinP1I GCGC 1 cut(s) 958
HindIII AAGCTT 1 cut(s) 123
HinfI GANTC 2 cut(s) 319, 988
HphI GGTGA 2 cut(s) 151, 355
Hpy166II GTNNAC 2 cut(s) 749, 881
Hpy188I TCNGA 2 cut(s) 34, 159
Hpy188III TCNNGA 3 cut(s) 182, 311, 828
Hpy8I GTNNAC 2 cut(s) 749, 881
HpyAV CCTTC 5 cut(s) 82, 86, 403, 591, 787
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4IV ACGT 2 cut(s) 691, 745
HpyCH4V TGCA 2 cut(s) 134, 590
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 199, 955
HpyF3I CTNAG 4 cut(s) 432, 452, 504, 624
HpySE526I ACGT 2 cut(s) 691, 745
Hsp92II CATG 2 cut(s) 478, 775
HspAI GCGC 1 cut(s) 958
LmnI GCTCC 5 cut(s) 61, 345, 698, 784, 923
Lsp1109I GCAGC 2 cut(s) 55, 186
MaeI CTAG 2 cut(s) 716, 930
MaeII ACGT 2 cut(s) 691, 745
MaeIII GTNAC 2 cut(s) 219, 370
MboII GAAGA 5 cut(s) 17, 401, 572, 689, 770
MhlI GDGCHC 3 cut(s) 273, 383, 781
MluCI AATT 6 cut(s) 16, 151, 499, 536, 856, 870
MmeI TCCRAC 3 cut(s) 182, 676, 892
Mph1103I ATGCAT 1 cut(s) 592
MroXI GAANNNNTTC 1 cut(s) 318
MseI TTAA 4 cut(s) 128, 818, 873, 980
MwoI GCNNNNNNNGC 3 cut(s) 131, 199, 955
NlaIII CATG 2 cut(s) 478, 775
NlaIV GGNNCC 2 cut(s) 272, 414
NmuCI GTSAC 1 cut(s) 219
NsiI ATGCAT 1 cut(s) 592
NspI RCATGY 1 cut(s) 478
PceI AGGCCT 1 cut(s) 673
PdmI GAANNNNTTC 1 cut(s) 318
PfeI GAWTC 2 cut(s) 319, 988
PkrI GCNGC 2 cut(s) 70, 201
PspFI CCCAGC 1 cut(s) 60
PspN4I GGNNCC 2 cut(s) 272, 414
PspPI GGNCC 1 cut(s) 398
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
SaqAI TTAA 4 cut(s) 128, 818, 873, 980
SatI GCNGC 2 cut(s) 69, 200
Sau96I GGNCC 1 cut(s) 398
SduI GDGCHC 3 cut(s) 273, 383, 781
Sse9I AATT 6 cut(s) 16, 151, 499, 536, 856, 870
SseBI AGGCCT 1 cut(s) 673
SsiI CCGC 2 cut(s) 460, 922
SspMI CTAG 2 cut(s) 716, 930
StuI AGGCCT 1 cut(s) 673
StyI CCWWGG 2 cut(s) 366, 885
TaaI ACNGT 1 cut(s) 106
TaiI ACGT 2 cut(s) 694, 748
TaqI TCGA 3 cut(s) 322, 526, 551
TasI AATT 6 cut(s) 16, 151, 499, 536, 856, 870
TfiI GAWTC 2 cut(s) 319, 988
Tru1I TTAA 4 cut(s) 128, 818, 873, 980
Tru9I TTAA 4 cut(s) 128, 818, 873, 980
TscAI CASTG 2 cut(s) 111, 931
TseFI GTSAC 1 cut(s) 219
TseI GCWGC 2 cut(s) 68, 199
Tsp45I GTSAC 1 cut(s) 219
TspDTI ATGAA 4 cut(s) 101, 756, 760, 882
TspGWI ACGGA 1 cut(s) 866
TspRI CASTG 2 cut(s) 111, 931
XapI RAATTY 4 cut(s) 16, 499, 536, 856
XceI RCATGY 1 cut(s) 478
XmnI GAANNNNTTC 1 cut(s) 318
XspI CTAG 2 cut(s) 716, 930
Zsp2I ATGCAT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.