pycom08g17840

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012995
Physical Location & Seq
Forward (+)
18054481 .. 18055998
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g17840.1

Sequence Viewer

Length: 1518 bp
ATGGACTCTTCCAATGGTTTCGAAGAATTTGAGAGTAACAATCATCTCCGCAGGTTGGTTAGAAATGGGGAGTTAGAAGAGGGATTTAAGTTTCTAGAGAACATGCTTTATCGCGGTGATATGCCGGATATTATTGCGTGTACGAGTTTAATCCGGGGGTTTTGTAAGAGTGGAAAGACAAAGAAGGCGACTCGGATTATGAACATTCTAGAAGACTCCGGGGCTGTCCTTGATGTTATAACTTACAATGTCTTGATCGGTGGCTACTGCAAGGCTGGTGAGATTGATAATGCCTTGAGGGTTTTGGACCGGATGAGTGTTTCTCCAGATGTTGTTACGTATAATACAATTTTGCGTACTTTGTGTGATAGTGGGAAATTGAAGCAAGCAATGGAAGTTCTTGATCGACAGATGCAAAGGCAATGTTATCCAGATGTGATTACATATACCATACTGATCGAAGCAACTTGTAAGGAAAGTGGGGTTGAGCAGGCGATGAAGCTTTTGGATGAGATGAGGAGCAAAGGATGCAAGCCTGATGTAGTCACTTATAATGTTCTTATAAATGGAATTTGCAAGGAAGGACGGTTGGATGAAGCAATCAAATTCTTGAACGGCATGCCATCATCTGATTGCCAACCAAATGTCATTACCCACAATATTATTTTGCGTAGCATGTGCAGCACCGGGAGGTGGATGGATGCTGAGAAACTATTAGCTGAAATGGTCCACAGAGGCTGTTCTCCCAGTGTTGTTACTTTCAATATTTTGATTAACTTCTTGTGCCGGAAGGGTTTATTGGGTAGAGCAATTGACATTTTGGAGAAGATGCCTAAGCACGGTTGTACACCAAACTCCTTGAGTTACAACCCATTGCTTCATGGCTTTTGCAAGGAAAAGAAGATGGATAGGGCGATTGAGTATTTGGATATAATGGTTTCTCGTGGCTGTTATCCTGACATTGTAACCTACAATACTTTGCTCACAGCTTTGTGCAAAGATGGAAAGGTTGATATCGCTGTTGAGATACTTAATCAACTAAGTAGCAAGGGTTGCTCTCCTGTTCTAATCACATACAACACAGTAATTGATGGGCTCTCAAAGGTGGGGAAAACAGAACGTGCCTTAGAACTTTTGCATGAGATGCGCAAAAAAGGTTTAAAGCCCGATGTAATTACTTATTCTTCTCTTGTGGGAGGGCTTAGCAGAGAAGGAAAGGTTGATGAGGCAATTAAGTTTGTCCATGATTTGGAAGATGCGGGCGTCAAGCCGAATGCTATCACTTTTAACTCCATCATGTTGGGGCTTTGCAAGGCTAAGCAAACTTGTCGTGCAATTGATTTCCTCGCTTATATGGTATCTAGAGGATGCAAACCTACTGAAGCAACATACACAATTCTCATTGAAGGCATAGCTTATGAAGGGTTAGCAAAAGAGGCTCTGGAGTTATTGAATGCATTGTGCTATAGAGGGGTTATGAAGAAAAGCTCTGCTGAACAAGTGGCAGTCAAGATGTAG

Protein Analysis

506

Amino Acids

56.14

Weight (kDa)

7.94

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 14 - 56 2e-06 PPR repeat family
PPR_long PF17177 24 - 138 6.2e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 42 - 91 1.3e-12 PPR repeat family
TPR_24 PF23276 55 - 167 3.9e-10 Fungal tetratrico peptide repeats
PPR_1 PF12854 74 - 106 3.5e-12 PPR repeat
PPR_2 PF13041 78 - 106 2.6e-07 PPR repeat family
PPR PF01535 80 - 105 2.4e-07 PPR repeat
PPR_3 PF13812 107 - 156 1.1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 107 - 135 2.1e-09 PPR repeat
PPR_2 PF13041 109 - 158 5.7e-15 PPR repeat family
TPR_24 PF23276 116 - 238 1.1e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 141 - 173 4.3e-10 PPR repeat
PPR_3 PF13812 144 - 191 4.6e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 144 - 193 7.6e-20 PPR repeat family
PPR PF01535 147 - 177 8.6e-06 PPR repeat
PPR_3 PF13812 167 - 224 5.9e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 175 - 207 5e-16 PPR repeat
PPR_2 PF13041 179 - 227 8.2e-18 PPR repeat family
PPR PF01535 182 - 212 3e-07 PPR repeat
PPR_3 PF13812 206 - 260 8.1e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 211 - 243 1.2e-08 PPR repeat
PPR_2 PF13041 218 - 263 1.7e-13 PPR repeat family
PPR_3 PF13812 237 - 295 3.8e-10 Pentatricopeptide repeat domain
PPR_long PF17177 237 - 350 5.3e-09 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 245 - 277 7.1e-12 PPR repeat
PPR_2 PF13041 249 - 298 9.2e-16 PPR repeat family
PPR PF01535 252 - 282 2.4e-06 PPR repeat
TPR_24 PF23276 263 - 380 6.4e-12 Fungal tetratrico peptide repeats
PPR_1 PF12854 281 - 313 2.8e-08 PPR repeat
PPR_2 PF13041 285 - 333 7.7e-16 PPR repeat family
PPR_3 PF13812 310 - 363 1.3e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 315 - 346 7.9e-12 PPR repeat
PPR_2 PF13041 319 - 354 3.8e-10 PPR repeat family
PPR PF01535 322 - 352 1.5e-06 PPR repeat
TPR_24 PF23276 334 - 447 6.3e-09 Fungal tetratrico peptide repeats
PPR_1 PF12854 350 - 383 8.7e-10 PPR repeat
PPR_2 PF13041 356 - 402 1.6e-16 PPR repeat family
PPR PF01535 357 - 387 5.2e-09 PPR repeat
PPR_3 PF13812 357 - 399 1.1e-08 Pentatricopeptide repeat domain
PPR_long PF17177 372 - 488 2e-10 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 385 - 416 3.4e-10 PPR repeat
PPR_2 PF13041 397 - 438 2.8e-09 PPR repeat family
PPR_3 PF13812 414 - 469 6.8e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 424 - 471 1.1e-13 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012995)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09900
fragaria_vesca FvH4_5g34030
malus_domestica MD08G1206600.v1.1 MD15G1390100.v1.1
prunus_persica Prupe.1G539000_v2.0.a1 Prupe.1G539000_v2.0.a1
pyrus_communis pycom08g17840 pycom15g34930
rosa_chinensis RchiOBHm_Chr7g0233521
rosa_laevigata RLG00000001253
rosa_multiflora Rmu_sc0000029.1_g000012
rosa_roxburghii Rroxscaffold_3G00227230
rosa_rugosa Rorug07G0281100.1
rosa_samantha Rh7AG437500 Rh7BG410000 Rh7CG457100 Rh7DG426500
rosa_wichuraiana Rw7G036080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 239, 552, 563
Acc16I TGCGCA 1 cut(s) 1148
Acc36I ACCTGC 1 cut(s) 42
AccB7I CCANNNNNTGG 1 cut(s) 1249
AccII CGCG 1 cut(s) 114
AciI CCGC 3 cut(s) 49, 114, 1259
AcsI RAATTY 3 cut(s) 26, 570, 605
AcuI CTGAAG 1 cut(s) 1401
AcyI GRCGYC 1 cut(s) 1263
AfaI GTAC 3 cut(s) 142, 358, 847
AfiI CCNNNNNNNGG 4 cut(s) 55, 693, 839, 1249
AgsI TTSAA 5 cut(s) 382, 613, 763, 1406, 1453
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
AluBI AGCT 5 cut(s) 502, 719, 989, 1415, 1488
AluI AGCT 5 cut(s) 502, 719, 989, 1415, 1488
AlwNI CAGNNNCTG 1 cut(s) 738
ApeKI GCWGC 1 cut(s) 681
ApoI RAATTY 3 cut(s) 26, 570, 605
AspLEI GCGC 1 cut(s) 1149
AspS9I GGNCC 2 cut(s) 307, 727
AsuC2I CCSGG 3 cut(s) 155, 220, 688
AsuHPI GGTGA 2 cut(s) 128, 290
AsuII TTCGAA 1 cut(s) 21
AvaII GGWCC 2 cut(s) 307, 727
BanII GRGCYC 1 cut(s) 1098
BauI CACGAG 1 cut(s) 942
BbsI GAAGAC 1 cut(s) 219
BbvI GCAGC 1 cut(s) 693
BccI CCATC 6 cut(s) 631, 691, 898, 995, 1085, 1301
BceAI ACGGC 1 cut(s) 631
BcgI CGANNNNNNTGC 2 cut(s) 1310, 1344
BcnI CCSGG 3 cut(s) 155, 220, 688
BfaI CTAG 3 cut(s) 95, 209, 1362
BfmI CTRYAG 1 cut(s) 1465
BfuAI ACCTGC 1 cut(s) 42
BisI GCNGC 1 cut(s) 682
BlpI GCTNAGC 2 cut(s) 1202, 1317
BlsI GCNGC 1 cut(s) 683
Bme1390I CCNGG 3 cut(s) 155, 220, 688
Bme18I GGWCC 2 cut(s) 307, 727
BmgT120I GGNCC 2 cut(s) 307, 727
BmrFI CCNGG 3 cut(s) 155, 220, 688
BmrI ACTGGG 1 cut(s) 741
BmsI GCATC 7 cut(s) 402, 518, 691, 819, 1134, 1246, 1358
BmuI ACTGGG 1 cut(s) 741
BpiI GAAGAC 1 cut(s) 219
BplI GAGNNNNNCTC 2 cut(s) 307, 339
BpmI CTGGAG 2 cut(s) 309, 1463
Bpu10I CCTNAGC 1 cut(s) 834
Bpu1102I GCTNAGC 2 cut(s) 1202, 1317
Bpu14I TTCGAA 1 cut(s) 21
BpuEI CTTGAG 2 cut(s) 316, 880
BpuMI CCSGG 3 cut(s) 155, 220, 688
BsaAI YACGTR 1 cut(s) 339
BsaHI GRCGYC 1 cut(s) 1263
BsaJI CCNNGG 2 cut(s) 154, 219
BsaWI WCCGGW 1 cut(s) 309
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 693, 839, 1249
Bse1I ACTGG 1 cut(s) 747
Bse3DI GCAATG 3 cut(s) 396, 428, 872
BseDI CCNNGG 2 cut(s) 154, 219
BseGI GGATG 7 cut(s) 318, 514, 533, 598, 702, 706, 1373
BseLI CCNNNNNNNGG 4 cut(s) 55, 693, 839, 1249
BseMI GCAATG 3 cut(s) 396, 428, 872
BseMII CTCAG 1 cut(s) 696
BseNI ACTGG 1 cut(s) 747
BseRI GAGGAG 1 cut(s) 532
BseXI GCAGC 1 cut(s) 693
BsgI GTGCAG 1 cut(s) 700
Bsh1236I CGCG 1 cut(s) 114
BsiSI CCGG 6 cut(s) 125, 154, 219, 310, 687, 787
BslI CCNNNNNNNGG 4 cut(s) 55, 693, 839, 1249
BsmI GAATGC 2 cut(s) 1279, 1459
Bsp119I TTCGAA 1 cut(s) 21
Bsp1286I GDGCHC 1 cut(s) 1098
Bsp1407I TGTACA 1 cut(s) 845
Bsp143I GATC 3 cut(s) 255, 403, 456
Bsp1720I GCTNAGC 2 cut(s) 1202, 1317
BspACI CCGC 3 cut(s) 49, 114, 1259
BspCNI CTCAG 1 cut(s) 697
BspFNI CGCG 1 cut(s) 114
BspMI ACCTGC 1 cut(s) 42
BspT104I TTCGAA 1 cut(s) 21
BsrDI GCAATG 3 cut(s) 396, 428, 872
BsrGI TGTACA 1 cut(s) 845
BsrI ACTGG 1 cut(s) 747
BssECI CCNNGG 2 cut(s) 154, 219
BssMI GATC 3 cut(s) 255, 403, 456
BssNI GRCGYC 1 cut(s) 1263
BssSI CACGAG 1 cut(s) 942
Bst2BI CACGAG 1 cut(s) 942
Bst4CI ACNGT 3 cut(s) 588, 842, 1084
Bst6I CTCTTC 2 cut(s) 13, 72
BstACI GRCGYC 1 cut(s) 1263
BstAPI GCANNNNNTGC 3 cut(s) 528, 1053, 1144
BstAUI TGTACA 1 cut(s) 845
BstBAI YACGTR 1 cut(s) 339
BstBI TTCGAA 1 cut(s) 21
BstC8I GCNNGC 5 cut(s) 387, 492, 533, 620, 1261
BstDEI CTNAG 6 cut(s) 705, 834, 1040, 1126, 1202, 1317
BstF5I GGATG 7 cut(s) 318, 514, 533, 598, 702, 706, 1373
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 1149
BstKTI GATC 3 cut(s) 258, 406, 459
BstMBI GATC 3 cut(s) 255, 403, 456
BstMWI GCNNNNNNNGC 5 cut(s) 528, 681, 1053, 1144, 1436
BstNSI RCATGY 3 cut(s) 106, 622, 679
BstSCI CCNGG 3 cut(s) 153, 218, 686
BstSFI CTRYAG 1 cut(s) 1465
BstSNI TACGTA 1 cut(s) 339
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 1 cut(s) 693
BstV2I GAAGAC 1 cut(s) 219
BstXI CCANNNNNNTGG 1 cut(s) 1300
BtgZI GCGATG 1 cut(s) 509
BtsCI GGATG 7 cut(s) 318, 514, 533, 598, 702, 706, 1373
BtsIMutI CAGTG 1 cut(s) 754
BveI ACCTGC 1 cut(s) 42
Cac8I GCNNGC 5 cut(s) 387, 492, 533, 620, 1261
CaiI CAGNNNCTG 1 cut(s) 738
CfoI GCGC 1 cut(s) 1149
Cfr13I GGNCC 2 cut(s) 307, 727
CseI GACGC 1 cut(s) 1252
Csp6I GTAC 3 cut(s) 141, 357, 846
CviAII CATG 7 cut(s) 103, 619, 676, 881, 1139, 1244, 1297
CviQI GTAC 3 cut(s) 141, 357, 846
DdeI CTNAG 6 cut(s) 705, 834, 1040, 1126, 1202, 1317
DpnI GATC 3 cut(s) 257, 405, 458
DpnII GATC 3 cut(s) 255, 403, 456
DraI TTTAAA 1 cut(s) 1161
Eam1104I CTCTTC 2 cut(s) 13, 72
EarI CTCTTC 2 cut(s) 13, 72
Eco105I TACGTA 1 cut(s) 339
Eco24I GRGCYC 1 cut(s) 1098
Eco32I GATATC 1 cut(s) 1015
Eco47I GGWCC 2 cut(s) 307, 727
Eco57I CTGAAG 1 cut(s) 1401
EcoRV GATATC 1 cut(s) 1015
EcoT22I ATGCAT 1 cut(s) 1459
EcoT38I GRGCYC 1 cut(s) 1098
FaeI CATG 7 cut(s) 106, 622, 679, 884, 1142, 1247, 1300
FatI CATG 7 cut(s) 102, 618, 675, 880, 1138, 1243, 1296
FauI CCCGC 1 cut(s) 1252
Fnu4HI GCNGC 1 cut(s) 682
FokI GGATG 7 cut(s) 325, 521, 540, 605, 709, 713, 1380
FriOI GRGCYC 1 cut(s) 1098
Fsp4HI GCNGC 1 cut(s) 682
FspBI CTAG 3 cut(s) 95, 209, 1362
FspI TGCGCA 1 cut(s) 1148
GlaI GCGC 1 cut(s) 1148
GluI GCNGC 1 cut(s) 682
GsuI CTGGAG 2 cut(s) 309, 1463
HapII CCGG 6 cut(s) 125, 154, 219, 310, 687, 787
HgaI GACGC 1 cut(s) 1252
HhaI GCGC 1 cut(s) 1149
Hin1I GRCGYC 1 cut(s) 1263
Hin1II CATG 7 cut(s) 106, 622, 679, 884, 1142, 1247, 1300
Hin6I GCGC 1 cut(s) 1147
HinP1I GCGC 1 cut(s) 1147
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 3 cut(s) 5, 190, 215
HpaII CCGG 6 cut(s) 125, 154, 219, 310, 687, 787
HphI GGTGA 2 cut(s) 128, 290
Hpy166II GTNNAC 3 cut(s) 141, 730, 848
Hpy188I TCNGA 2 cut(s) 195, 631
Hpy8I GTNNAC 3 cut(s) 141, 730, 848
HpyAV CCTTC 6 cut(s) 178, 575, 784, 1205, 1400, 1415
HpyCH4III ACNGT 3 cut(s) 588, 842, 1084
HpyCH4IV ACGT 2 cut(s) 338, 1120
HpyF10VI GCNNNNNNNGC 5 cut(s) 528, 681, 1053, 1144, 1436
HpyF3I CTNAG 6 cut(s) 705, 834, 1040, 1126, 1202, 1317
HpySE526I ACGT 2 cut(s) 338, 1120
Hsp92I GRCGYC 1 cut(s) 1263
Hsp92II CATG 7 cut(s) 106, 622, 679, 884, 1142, 1247, 1300
HspAI GCGC 1 cut(s) 1147
Kzo9I GATC 3 cut(s) 255, 403, 456
LmnI GCTCC 1 cut(s) 519
Lsp1109I GCAGC 1 cut(s) 693
LweI GCATC 7 cut(s) 402, 518, 691, 819, 1134, 1246, 1358
MaeI CTAG 3 cut(s) 95, 209, 1362
MaeII ACGT 2 cut(s) 338, 1120
MaeIII GTNAC 6 cut(s) 35, 334, 544, 754, 863, 964
MalI GATC 3 cut(s) 257, 405, 458
MboI GATC 3 cut(s) 255, 403, 456
MboII GAAGA 8 cut(s) 35, 89, 224, 838, 913, 1176, 1265, 1492
MfeI CAATTG 2 cut(s) 810, 1335
MhlI GDGCHC 1 cut(s) 1098
MlyI GAGTC 2 cut(s) 184, 209
MmeI TCCRAC 1 cut(s) 570
Mph1103I ATGCAT 1 cut(s) 1459
MseI TTAA 7 cut(s) 87, 149, 774, 1032, 1160, 1233, 1287
MspI CCGG 6 cut(s) 125, 154, 219, 310, 687, 787
MspR9I CCNGG 3 cut(s) 155, 220, 688
MunI CAATTG 2 cut(s) 810, 1335
Mva1269I GAATGC 2 cut(s) 1279, 1459
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 5 cut(s) 528, 681, 1053, 1144, 1436
NciI CCSGG 3 cut(s) 155, 220, 688
NdeII GATC 3 cut(s) 255, 403, 456
NlaIII CATG 7 cut(s) 106, 622, 679, 884, 1142, 1247, 1300
NmuCI GTSAC 1 cut(s) 544
NsbI TGCGCA 1 cut(s) 1148
NsiI ATGCAT 1 cut(s) 1459
NspI RCATGY 3 cut(s) 106, 622, 679
NspV TTCGAA 1 cut(s) 21
PaeI GCATGC 1 cut(s) 622
PctI GAATGC 2 cut(s) 1279, 1459
PflMI CCANNNNNTGG 1 cut(s) 1249
PkrI GCNGC 1 cut(s) 683
PleI GAGTC 2 cut(s) 184, 209
PpsI GAGTC 2 cut(s) 184, 209
Ppu21I YACGTR 1 cut(s) 339
PsiI TTATAA 3 cut(s) 239, 552, 563
PspPI GGNCC 2 cut(s) 307, 727
PstNI CAGNNNCTG 1 cut(s) 738
RsaI GTAC 3 cut(s) 142, 358, 847
RsaNI GTAC 3 cut(s) 141, 357, 846
SaqAI TTAA 7 cut(s) 87, 149, 774, 1032, 1160, 1233, 1287
SatI GCNGC 1 cut(s) 682
Sau3AI GATC 3 cut(s) 255, 403, 456
Sau96I GGNCC 2 cut(s) 307, 727
SchI GAGTC 2 cut(s) 184, 209
ScrFI CCNGG 3 cut(s) 155, 220, 688
SduI GDGCHC 1 cut(s) 1098
SfaNI GCATC 7 cut(s) 402, 518, 691, 819, 1134, 1246, 1358
SfcI CTRYAG 1 cut(s) 1465
SfuI TTCGAA 1 cut(s) 21
SinI GGWCC 2 cut(s) 307, 727
SmlI CTYRAG 2 cut(s) 295, 859
SmoI CTYRAG 2 cut(s) 295, 859
SnaBI TACGTA 1 cut(s) 339
SphI GCATGC 1 cut(s) 622
SsiI CCGC 3 cut(s) 49, 114, 1259
SspI AATATT 2 cut(s) 661, 766
SspMI CTAG 3 cut(s) 95, 209, 1362
StyD4I CCNGG 3 cut(s) 153, 218, 686
TaaI ACNGT 3 cut(s) 588, 842, 1084
TaiI ACGT 2 cut(s) 341, 1123
TaqI TCGA 3 cut(s) 21, 406, 459
TatI WGTACW 1 cut(s) 845
Tru1I TTAA 7 cut(s) 87, 149, 774, 1032, 1160, 1233, 1287
Tru9I TTAA 7 cut(s) 87, 149, 774, 1032, 1160, 1233, 1287
TscAI CASTG 1 cut(s) 754
TseFI GTSAC 1 cut(s) 544
TseI GCWGC 1 cut(s) 681
Tsp45I GTSAC 1 cut(s) 544
TspDTI ATGAA 6 cut(s) 215, 512, 609, 869, 1434, 1493
TspRI CASTG 1 cut(s) 754
Van91I CCANNNNNTGG 1 cut(s) 1249
VpaK11BI GGWCC 2 cut(s) 307, 727
XapI RAATTY 3 cut(s) 26, 570, 605
XbaI TCTAGA 3 cut(s) 94, 208, 1361
XceI RCATGY 3 cut(s) 106, 622, 679
XspI CTAG 3 cut(s) 95, 209, 1362
Zsp2I ATGCAT 1 cut(s) 1459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.