pycom08g20320

Tumour-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008622
Physical Location & Seq
Forward (+)
20154717 .. 20162274
7558 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1167 bp
ATGGATGCGGAGCAGACCTTCATCCGAGTACAAGAGCGGTTCTCGCAGATCCTGACGCCGAAAGTGAGAGCTGCTTTGGAGTATATATACCTCTTCATCGCCATCACCTTGTTCTTTATTCTCGTTGTGATGCACGCCAATTACGTTCAACAGCCTGGCTGTTCAAGTGAACTTTCTGGTGTCGAAACAACAGAAGCCCAAATTATTCAAATTAAGATAACTAGTGCAGGGTTGTGGTCGCAGAGTGAGTCAGAGTTCACCAATGTGATGGATGATGCCCCAGACACAAAATCTGTAATGAGTGGTAAGTTGGAACTGAAGACTGATACTGAAGTTCTTGATCGACAGACAGATAGCTCTACCGATAGCCTGAATACTATGGCAACACTTGATGATGCCGTTGTCAAAAATGAAATAAAGGAGAGACGTAGTAGTTTTCTTTCACCTATGAAAGATACAGTTAGAGCCGCACTTGTTAACTTTATCAAACTGTGGAAAATGCGTCCCCGTCTCTTTTCAGTCTTTCGGAAACATGGAGCACATGTCATTGGAAATTTATGGAACATTGCAGGGATACATTTAGATCTTGATATTCCCAAGTGGTTGCGCATACTTCATTTGGACAGGCTTAACTTGTATGCAGTGCAGTGGCTTGAGACCAGAAGCAAAACTTTTGAACCAACCTATTTGTACACTATGGAAAAGGGTTATTTCTTACTACCTGAAAGTGCCAGGTCTCGGCATAATATTCGTGCTGTAAACATTAGCATATCAGCTCGGCATTCTTGTTTTGGGAACAGGTGGCAGCAGCTACTCATTAACAGATTTGTTGGATATGATACTATTTTAATAAATAGTTTATTGAGTTCACCTGGACAAGGATATCTTTATAATTTTCAGACAAAGGAGTTTTTTAATATCAGCTATGCACAAGAACCGCCACAAGGCCCTGCAAGGTTTGGAGACTACCTTGTGACAAAATGTGGTGTGCTTATGATGTCCATTTTTGTGTTCTTTACAACCACCATGTCGGTGTCATTCACATTGAGAGAGACACAGACTCGCATGCTGAAGTTCACAGTGCAGCTTCAACACCATGCTCGTCATCAGCTTCCAACTTTCCAGTTAATTTTTGTGCATGTAATTGAATCACTTGTCTTTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Membralin

Protein Analysis

389

Amino Acids

44.74

Weight (kDa)

8.82

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Membralin PF09746 7 - 363 1.2e-19 Tumour-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 891
Acc16I TGCGCA 1 cut(s) 608
AccBSI CCGCTC 1 cut(s) 37
AciI CCGC 4 cut(s) 8, 37, 468, 938
AclWI GGATC 1 cut(s) 43
AcsI RAATTY 1 cut(s) 553
AcuI CTGAAG 3 cut(s) 338, 351, 1091
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 2 cut(s) 30, 692
AfiI CCNNNNNNNGG 3 cut(s) 738, 878, 944
AflIII ACRYGT 1 cut(s) 541
AgsI TTSAA 6 cut(s) 149, 165, 209, 677, 1091, 1148
AhlI ACTAGT 1 cut(s) 221
AjnI CCWGG 3 cut(s) 154, 731, 871
AleI CACNNNNGTG 1 cut(s) 263
AluBI AGCT 7 cut(s) 71, 357, 776, 811, 924, 1087, 1111
AluI AGCT 7 cut(s) 71, 357, 776, 811, 924, 1087, 1111
Alw21I GWGCWC 1 cut(s) 541
Alw26I GTCTC 6 cut(s) 418, 515, 650, 741, 957, 1046
AlwI GGATC 1 cut(s) 43
AlwNI CAGNNNCTG 1 cut(s) 52
AoxI GGCC 1 cut(s) 946
ApeKI GCWGC 4 cut(s) 71, 805, 808, 1084
ApoI RAATTY 1 cut(s) 553
AspLEI GCGC 1 cut(s) 609
AspS9I GGNCC 1 cut(s) 947
AsuHPI GGTGA 4 cut(s) 97, 250, 435, 861
BaeI ACNNNNGTAYC 2 cut(s) 318, 351
BbsI GAAGAC 1 cut(s) 326
Bbv12I GWGCWC 1 cut(s) 541
BbvI GCAGC 4 cut(s) 58, 817, 820, 1096
BccI CCATC 2 cut(s) 110, 262
BceAI ACGGC 1 cut(s) 383
BcgI CGANNNNNNTGC 2 cut(s) 731, 765
BciT130I CCWGG 3 cut(s) 156, 733, 873
BciVI GTATCC 1 cut(s) 567
BcoDI GTCTC 6 cut(s) 418, 515, 650, 741, 957, 1046
BcuI ACTAGT 1 cut(s) 221
BfaI CTAG 1 cut(s) 222
BfuI GTATCC 1 cut(s) 567
BglII AGATCT 1 cut(s) 583
BisI GCNGC 5 cut(s) 72, 468, 806, 809, 1085
BlsI GCNGC 5 cut(s) 73, 469, 807, 810, 1086
Bme1390I CCNGG 3 cut(s) 156, 733, 873
BmgT120I GGNCC 1 cut(s) 947
BmrFI CCNGG 3 cut(s) 156, 733, 873
BmsI GCATC 3 cut(s) 120, 265, 385
BpiI GAAGAC 1 cut(s) 326
BplI GAGNNNNNCTC 2 cut(s) 26, 58
BpuEI CTTGAG 1 cut(s) 674
BsaHI GRCGYC 1 cut(s) 56
BsaI GGTCTC 2 cut(s) 650, 741
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bsc4I CCNNNNNNNGG 3 cut(s) 738, 878, 944
Bse1I ACTGG 1 cut(s) 1123
Bse3DI GCAATG 1 cut(s) 564
BseBI CCWGG 3 cut(s) 156, 733, 873
BseGI GGATG 3 cut(s) 10, 21, 277
BseLI CCNNNNNNNGG 3 cut(s) 738, 878, 944
BseMI GCAATG 1 cut(s) 564
BseNI ACTGG 1 cut(s) 1123
BseXI GCAGC 4 cut(s) 58, 817, 820, 1096
BsgI GTGCAG 3 cut(s) 246, 665, 1103
BshFI GGCC 1 cut(s) 948
BsiHKAI GWGCWC 1 cut(s) 541
BslFI GGGAC 1 cut(s) 489
BslI CCNNNNNNNGG 3 cut(s) 738, 878, 944
BsmAI GTCTC 6 cut(s) 418, 515, 650, 741, 957, 1046
BsmBI CGTCTC 2 cut(s) 418, 515
BsmFI GGGAC 1 cut(s) 489
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 1 cut(s) 948
Bso31I GGTCTC 2 cut(s) 650, 741
Bsp1286I GDGCHC 1 cut(s) 541
Bsp1407I TGTACA 1 cut(s) 690
Bsp143I GATC 3 cut(s) 48, 340, 583
BspACI CCGC 4 cut(s) 8, 37, 468, 938
BspANI GGCC 1 cut(s) 948
BspPI GGATC 1 cut(s) 43
BspTNI GGTCTC 2 cut(s) 650, 741
BsrBI CCGCTC 1 cut(s) 37
BsrDI GCAATG 1 cut(s) 564
BsrGI TGTACA 1 cut(s) 690
BsrI ACTGG 1 cut(s) 1123
BssMI GATC 3 cut(s) 48, 340, 583
BssNI GRCGYC 1 cut(s) 56
Bst2UI CCWGG 3 cut(s) 156, 733, 873
Bst4CI ACNGT 3 cut(s) 460, 492, 1081
Bst6I CTCTTC 1 cut(s) 98
BstACI GRCGYC 1 cut(s) 56
BstAUI TGTACA 1 cut(s) 690
BstC8I GCNNGC 2 cut(s) 135, 1067
BstENI CCTNNNNNAGG 1 cut(s) 876
BstF5I GGATG 3 cut(s) 10, 21, 277
BstHHI GCGC 1 cut(s) 609
BstKTI GATC 3 cut(s) 51, 343, 586
BstMAI GTCTC 6 cut(s) 418, 515, 650, 741, 957, 1046
BstMBI GATC 3 cut(s) 48, 340, 583
BstMWI GCNNNNNNNGC 1 cut(s) 43
BstNI CCWGG 3 cut(s) 156, 733, 873
BstNSI RCATGY 3 cut(s) 545, 1069, 1142
BstSCI CCNGG 3 cut(s) 154, 731, 871
BstV1I GCAGC 4 cut(s) 58, 817, 820, 1096
BstV2I GAAGAC 1 cut(s) 326
BstX2I RGATCY 2 cut(s) 48, 583
BstXI CCANNNNNNTGG 1 cut(s) 268
BstYI RGATCY 2 cut(s) 48, 583
BsuI GTATCC 1 cut(s) 567
BsuRI GGCC 1 cut(s) 948
BtgZI GCGATG 1 cut(s) 82
BtsCI GGATG 3 cut(s) 10, 21, 277
BtsI GCAGTG 2 cut(s) 648, 653
BtsIMutI CAGTG 3 cut(s) 648, 653, 1086
Cac8I GCNNGC 2 cut(s) 135, 1067
CaiI CAGNNNCTG 1 cut(s) 52
CfoI GCGC 1 cut(s) 609
Cfr13I GGNCC 1 cut(s) 947
CseI GACGC 2 cut(s) 64, 491
Csp6I GTAC 2 cut(s) 29, 691
CviAII CATG 6 cut(s) 533, 542, 1027, 1066, 1097, 1139
CviQI GTAC 2 cut(s) 29, 691
DpnI GATC 3 cut(s) 50, 342, 585
DpnII GATC 3 cut(s) 48, 340, 583
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
Eco31I GGTCTC 2 cut(s) 650, 741
Eco32I GATATC 1 cut(s) 884
Eco57I CTGAAG 3 cut(s) 338, 351, 1091
EcoNI CCTNNNNNAGG 1 cut(s) 876
EcoO109I RGGNCCY 1 cut(s) 947
EcoRII CCWGG 3 cut(s) 154, 731, 871
EcoRV GATATC 1 cut(s) 884
Esp3I CGTCTC 2 cut(s) 418, 515
FaeI CATG 6 cut(s) 536, 545, 1030, 1069, 1100, 1142
FalI AAGNNNNNCTT 4 cut(s) 655, 687, 870, 902
FaqI GGGAC 1 cut(s) 489
FatI CATG 6 cut(s) 532, 541, 1026, 1065, 1096, 1138
Fnu4HI GCNGC 5 cut(s) 72, 468, 806, 809, 1085
FokI GGATG 3 cut(s) 8, 17, 284
Fsp4HI GCNGC 5 cut(s) 72, 468, 806, 809, 1085
FspBI CTAG 1 cut(s) 222
FspI TGCGCA 1 cut(s) 608
GlaI GCGC 1 cut(s) 608
GluI GCNGC 5 cut(s) 72, 468, 806, 809, 1085
HaeIII GGCC 1 cut(s) 948
HgaI GACGC 2 cut(s) 64, 491
HhaI GCGC 1 cut(s) 609
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 6 cut(s) 536, 545, 1030, 1069, 1100, 1142
Hin6I GCGC 1 cut(s) 607
HinP1I GCGC 1 cut(s) 607
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HinfI GANTC 3 cut(s) 248, 1060, 1148
HpaI GTTAAC 1 cut(s) 478
HphI GGTGA 4 cut(s) 97, 250, 435, 861
Hpy166II GTNNAC 7 cut(s) 170, 258, 478, 693, 760, 869, 1077
Hpy188I TCNGA 4 cut(s) 26, 253, 528, 900
Hpy188III TCNNGA 3 cut(s) 52, 338, 587
Hpy8I GTNNAC 7 cut(s) 170, 258, 478, 693, 760, 869, 1077
HpyAV CCTTC 1 cut(s) 28
HpyCH4III ACNGT 3 cut(s) 460, 492, 1081
HpyCH4IV ACGT 2 cut(s) 144, 427
HpyCH4V TGCA 9 cut(s) 133, 227, 569, 641, 646, 929, 953, 1084, 1138
HpyF10VI GCNNNNNNNGC 1 cut(s) 43
HpySE526I ACGT 2 cut(s) 144, 427
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 6 cut(s) 536, 545, 1030, 1069, 1100, 1142
HspAI GCGC 1 cut(s) 607
KspAI GTTAAC 1 cut(s) 478
Kzo9I GATC 3 cut(s) 48, 340, 583
LmnI GCTCC 2 cut(s) 10, 536
Lsp1109I GCAGC 4 cut(s) 58, 817, 820, 1096
LweI GCATC 3 cut(s) 120, 265, 385
MaeI CTAG 1 cut(s) 222
MaeII ACGT 2 cut(s) 144, 427
MaeIII GTNAC 1 cut(s) 973
MalI GATC 3 cut(s) 50, 342, 585
MbiI CCGCTC 1 cut(s) 37
MboI GATC 3 cut(s) 48, 340, 583
MboII GAAGA 2 cut(s) 85, 331
MflI RGATCY 2 cut(s) 48, 583
MhlI GDGCHC 1 cut(s) 541
MluCI AATT 7 cut(s) 139, 201, 210, 553, 892, 1128, 1143
MlyI GAGTC 2 cut(s) 257, 1054
MmeI TCCRAC 3 cut(s) 291, 811, 1139
MnlI CCTC 1 cut(s) 101
MseI TTAA 7 cut(s) 213, 477, 630, 819, 848, 915, 1127
MslI CAYNNNNRTG 3 cut(s) 263, 1007, 1031
MspR9I CCNGG 3 cut(s) 156, 733, 873
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 3 cut(s) 156, 733, 873
MwoI GCNNNNNNNGC 1 cut(s) 43
NdeII GATC 3 cut(s) 48, 340, 583
NlaIII CATG 6 cut(s) 536, 545, 1030, 1069, 1100, 1142
NmeAIII GCCGAG 2 cut(s) 718, 757
NmuCI GTSAC 1 cut(s) 973
NsbI TGCGCA 1 cut(s) 608
NspI RCATGY 3 cut(s) 545, 1069, 1142
OliI CACNNNNGTG 1 cut(s) 263
PaeI GCATGC 1 cut(s) 1069
PciI ACATGT 1 cut(s) 541
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 1 cut(s) 781
PfeI GAWTC 1 cut(s) 1148
PkrI GCNGC 5 cut(s) 73, 469, 807, 810, 1086
PleI GAGTC 2 cut(s) 256, 1054
PpsI GAGTC 2 cut(s) 256, 1054
PscI ACATGT 1 cut(s) 541
PsiI TTATAA 1 cut(s) 891
Psp6I CCWGG 3 cut(s) 154, 731, 871
PspGI CCWGG 3 cut(s) 154, 731, 871
PspPI GGNCC 1 cut(s) 947
PstNI CAGNNNCTG 1 cut(s) 52
PsuI RGATCY 2 cut(s) 48, 583
RsaI GTAC 2 cut(s) 30, 692
RsaNI GTAC 2 cut(s) 29, 691
RseI CAYNNNNRTG 3 cut(s) 263, 1007, 1031
SaqAI TTAA 7 cut(s) 213, 477, 630, 819, 848, 915, 1127
SatI GCNGC 5 cut(s) 72, 468, 806, 809, 1085
Sau3AI GATC 3 cut(s) 48, 340, 583
Sau96I GGNCC 1 cut(s) 947
SchI GAGTC 2 cut(s) 257, 1054
ScrFI CCNGG 3 cut(s) 156, 733, 873
SduI GDGCHC 1 cut(s) 541
SfaNI GCATC 3 cut(s) 120, 265, 385
SmiMI CAYNNNNRTG 3 cut(s) 263, 1007, 1031
SmlI CTYRAG 1 cut(s) 653
SmoI CTYRAG 1 cut(s) 653
SpeI ACTAGT 1 cut(s) 221
SphI GCATGC 1 cut(s) 1069
Sse9I AATT 7 cut(s) 139, 201, 210, 553, 892, 1128, 1143
SsiI CCGC 4 cut(s) 8, 37, 468, 938
SspI AATATT 1 cut(s) 748
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 3 cut(s) 154, 731, 871
TaaI ACNGT 3 cut(s) 460, 492, 1081
TaiI ACGT 2 cut(s) 147, 430
TaqI TCGA 2 cut(s) 183, 343
TasI AATT 7 cut(s) 139, 201, 210, 553, 892, 1128, 1143
TatI WGTACW 2 cut(s) 28, 690
TauI GCSGC 1 cut(s) 470
TfiI GAWTC 1 cut(s) 1148
Tru1I TTAA 7 cut(s) 213, 477, 630, 819, 848, 915, 1127
Tru9I TTAA 7 cut(s) 213, 477, 630, 819, 848, 915, 1127
TscAI CASTG 3 cut(s) 648, 653, 1086
TseFI GTSAC 1 cut(s) 973
TseI GCWGC 4 cut(s) 71, 805, 808, 1084
Tsp45I GTSAC 1 cut(s) 973
TspDTI ATGAA 5 cut(s) 10, 85, 426, 464, 605
TspRI CASTG 3 cut(s) 648, 653, 1086
XagI CCTNNNNNAGG 1 cut(s) 876
XapI RAATTY 1 cut(s) 553
XceI RCATGY 3 cut(s) 545, 1069, 1142
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.