Rw1G010050

Tumour-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008622
Physical Location & Seq
Forward (+)
22348707 .. 22358079
9373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G010050.1

Sequence Viewer

Length: 1890 bp
ATGGATCCGGAGCAGACATTCATACGCGTACAAGAGCGCTTCTCTCAGATCCTGACGCCAAAAGTGAGAGCTGCTTTGGAGTATATCTACCTTTTCATCGCGATCACTCTCTTCTGTATTCTCGTTGTTATGCACGCTAATTACGTTCAGCAGCCTGGCTGTTCAAGTGAGCTTCCTGGAGTTGAAACAGCAGACGCAGAAATTATTCATGTTAAGATTTCCAGTGCAGGCTTGTGGTCGCAGAATGAGTCAGAATTCACCAATGTGATAAATGATGTCCCCGAGACGAAATTTGTGGTGGATAAATTAGAATTTGCCAATGTAAATGGGGATGAGATTCTGGATCATCAGGCAGATAGCTCTATGCCAAATGTTGATGATGCGGTTATGAAAACTGAAATAGAGGAGTCACGTAGTAGTTTTCCTTTATCTGCAAAAGAGACATTCAAAGCAGCACTCTTTCATTTTAGCAACAAGTGGCACAGGCGTCTATCATTCCTTTGGAAGCATGGAACACATATTGTTGGAAGTTTGTGGAACATTGCAGGCATACATTTGGATCTTGATATTCCAAAGTGGCTGCATATACTTCGATTGGACAGGCTTAACTCTTATGCAGTGCAGTGGCTTGAGAACAGAAGCAAAACTTTTGAACCAACTTACTTATACACTATGGAAAAGGGTTATTTCTTACTGCCTGAAAGTGCAAGAGCTCAGCATAATATTCGTACTGTAAACATAAGCATATCAGCTCGACATTCCTGTTTCGGAAACAGGTGGCAGCAGCTACTGATTAACAGATTTGTTGGGTACGATACTATTTTGATAAACAGTTTACTGAGTTCTCCTGGTCAAGGTTATCTTTATAATTTTCAAACAAAGGAGTTCTTTAATCTTAGTTATGCACATGAACCGCCAGAGGGTCCGGCAAGATTTGGAGACTACCTCGTGACAAAGTGTGGCGTTCTTATGATGTCTCTATTTGTGTTCTTTACAACCACTATGTCTGTGTCATTTACTTTGAGAGAGACGCAGACTCGGATGCTGAAGTTCACAGTGCAGCTTCAACACCATGCTCGTCATCAGCTTCCAACATTTCAGTTGATTTTTGTGCATGTAATTGAATCACTTGTCTTTGTCCCGATTATGATTGGCATATTGTTTTTTCTGTTCGAGTTTTACGATGATCAACTATTGGCCTTCATGGTCCTAATTCTTGTCTGGTTGAGTGAACTATTTACCCTGATCAGTGTTCGCACGCCCATATCAATGAAGTTCTTTCCCCGCTTCTTTCTGCTCTACTTTCTGGTTTTTCACATCTATTTCTTTTCCTATGCTTACGGTTTTTCCTATTTGGCTCTTTCTACGGCTGCAGCATTTATGCAGCATCTCATTCTATACTTCTGGAACCGTTTTGAGGTACCAACTCTACAAAGGTTTATTCAAAATCGAAGATCACAACTCCAACAACACCCAGATTTCCACATTACTTCCTCCACATTCCTTGCCTCAACTTTGCATATCACAAGATTAAACACAAGAAATCCTGGTCCAGTCAATTCTGACATAGTCTCTGGGCCTGGGCTGAGATCTGGATCCAATCCAGCAGTGCCTTCAAATGGAGCTGAAGCTCCTGGCTTTCATGAACGATCAGGCAATGAGAACCCAGTGCAAAACCCTCTTCAGGTTCCAGGCCAAACTGACCTGAATCAAGCAGAGAATGGCCCAAACCCCAGCACTATGAATTCTTTCAGTTCACTTTTGTTGTGGATATTAGGAGGAGCTTCCTCTGAAGGCCTCAACTCATTTCTTTCTATGTTCAGAGATGTCCGAGACCAAGGACAAGCTTATGCTGAATCTCCCAGGCAAGAAAATCAGGATGGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Membralin

Protein Analysis

629

Amino Acids

72.1

Weight (kDa)

6.26

Isoelectric Point (pI)

53.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Membralin PF09746 7 - 424 4e-24 Tumour-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 867
Acc65I GGTACC 1 cut(s) 1420
AccB1I GGYRCC 1 cut(s) 1420
AccII CGCG 2 cut(s) 27, 101
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 3 cut(s) 383, 914, 1285
AclWI GGATC 6 cut(s) 12, 43, 351, 567, 1590, 1603
AcsI RAATTY 4 cut(s) 254, 290, 311, 1744
AcuI CTGAAG 4 cut(s) 1067, 1647, 1667, 1812
AcyI GRCGYC 2 cut(s) 56, 487
AfaI GTAC 4 cut(s) 30, 730, 812, 1422
AfeI AGCGCT 1 cut(s) 38
AfiI CCNNNNNNNGG 5 cut(s) 854, 920, 1417, 1619, 1684
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 9 cut(s) 165, 185, 448, 653, 875, 1067, 1124, 1445, 1617
AjnI CCWGG 8 cut(s) 154, 175, 847, 1546, 1579, 1633, 1690, 1862
AleI CACNNNNGTG 1 cut(s) 263
Alw21I GWGCWC 1 cut(s) 715
Alw26I GTCTC 7 cut(s) 278, 434, 933, 981, 1022, 1576, 1827
AlwI GGATC 6 cut(s) 12, 43, 351, 567, 1590, 1603
AlwNI CAGNNNCTG 2 cut(s) 52, 790
Ama87I CYCGRG 1 cut(s) 281
Aor13HI TCCGGA 1 cut(s) 7
Aor51HI AGCGCT 1 cut(s) 38
AoxI GGCC 5 cut(s) 1197, 1577, 1693, 1723, 1795
ApoI RAATTY 4 cut(s) 254, 290, 311, 1744
Asp700I GAANNNNTTC 2 cut(s) 204, 1748
Asp718I GGTACC 1 cut(s) 1420
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 5 cut(s) 923, 1207, 1550, 1577, 1724
AsuHPI GGTGA 1 cut(s) 250
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 3 cut(s) 923, 1207, 1550
BamHI GGATCC 2 cut(s) 4, 1595
BanI GGYRCC 1 cut(s) 1420
BanII GRGCYC 1 cut(s) 715
BauI CACGAG 1 cut(s) 947
Bbv12I GWGCWC 1 cut(s) 715
BccI CCATC 1 cut(s) 1874
BceAI ACGGC 1 cut(s) 1383
BcgI CGANNNNNNTGC 4 cut(s) 572, 606, 707, 741
BciT130I CCWGG 8 cut(s) 156, 177, 849, 1548, 1581, 1635, 1692, 1864
BclI TGATCA 2 cut(s) 1186, 1245
BcoDI GTCTC 7 cut(s) 278, 434, 933, 981, 1022, 1576, 1827
BfmI CTRYAG 1 cut(s) 1371
BfoI RGCGCY 1 cut(s) 40
BglII AGATCT 1 cut(s) 1589
BlpI GCTNAGC 1 cut(s) 714
Bme1390I CCNGG 8 cut(s) 156, 177, 849, 1548, 1581, 1635, 1692, 1864
Bme18I GGWCC 3 cut(s) 923, 1207, 1550
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 5 cut(s) 923, 1207, 1550, 1577, 1724
BmiI GGNNCC 6 cut(s) 6, 924, 1409, 1422, 1597, 1689
BmrFI CCNGG 8 cut(s) 156, 177, 849, 1548, 1581, 1635, 1692, 1864
BmrI ACTGGG 1 cut(s) 1661
BmsI GCATC 3 cut(s) 370, 1032, 1396
BmuI ACTGGG 1 cut(s) 1661
BplI GAGNNNNNCTC 4 cut(s) 26, 58, 930, 962
BpmI CTGGAG 1 cut(s) 198
Bpu1102I GCTNAGC 1 cut(s) 714
BpuEI CTTGAG 1 cut(s) 650
BsaAI YACGTR 1 cut(s) 413
BsaBI GATNNNNATC 1 cut(s) 1594
BsaHI GRCGYC 2 cut(s) 56, 487
BsaI GGTCTC 1 cut(s) 1827
BsaJI CCNNGG 3 cut(s) 1580, 1837, 1862
BsaWI WCCGGW 1 cut(s) 7
Bsc4I CCNNNNNNNGG 5 cut(s) 854, 920, 1417, 1619, 1684
Bse1I ACTGG 3 cut(s) 222, 1553, 1667
Bse3DI GCAATG 2 cut(s) 540, 1663
Bse8I GATNNNNATC 1 cut(s) 1594
BseAI TCCGGA 1 cut(s) 7
BseBI CCWGG 8 cut(s) 156, 177, 849, 1548, 1581, 1635, 1692, 1864
BseDI CCNNGG 3 cut(s) 1580, 1837, 1862
BseGI GGATG 3 cut(s) 337, 1047, 1885
BseJI GATNNNNATC 1 cut(s) 1594
BseLI CCNNNNNNNGG 5 cut(s) 854, 920, 1417, 1619, 1684
BseMI GCAATG 2 cut(s) 540, 1663
BseMII CTCAG 4 cut(s) 59, 728, 830, 1577
BseNI ACTGG 3 cut(s) 222, 1553, 1667
BseRI GAGGAG 2 cut(s) 419, 1794
BseYI CCCAGC 1 cut(s) 1733
BsgI GTGCAG 3 cut(s) 246, 641, 1079
Bsh1236I CGCG 2 cut(s) 27, 101
BshFI GGCC 5 cut(s) 1199, 1579, 1695, 1725, 1797
BshNI GGYRCC 1 cut(s) 1420
BsiHKAI GWGCWC 1 cut(s) 715
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 2 cut(s) 8, 926
BslFI GGGAC 2 cut(s) 263, 1124
BslI CCNNNNNNNGG 5 cut(s) 854, 920, 1417, 1619, 1684
BsmAI GTCTC 7 cut(s) 278, 434, 933, 981, 1022, 1576, 1827
BsmBI CGTCTC 2 cut(s) 278, 1022
BsmFI GGGAC 2 cut(s) 263, 1124
BsnI GGCC 5 cut(s) 1199, 1579, 1695, 1725, 1797
Bso31I GGTCTC 1 cut(s) 1827
BsoBI CYCGRG 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 715
Bsp13I TCCGGA 1 cut(s) 7
Bsp1720I GCTNAGC 1 cut(s) 714
Bsp68I TCGCGA 1 cut(s) 101
BspACI CCGC 3 cut(s) 383, 914, 1285
BspANI GGCC 5 cut(s) 1199, 1579, 1695, 1725, 1797
BspCNI CTCAG 4 cut(s) 58, 727, 831, 1578
BspEI TCCGGA 1 cut(s) 7
BspFNI CGCG 2 cut(s) 27, 101
BspHI TCATGA 1 cut(s) 1642
BspLI GGNNCC 6 cut(s) 6, 924, 1409, 1422, 1597, 1689
BspMAI CTGCAG 1 cut(s) 1375
BspPI GGATC 6 cut(s) 12, 43, 351, 567, 1590, 1603
BspT107I GGYRCC 1 cut(s) 1420
BspTNI GGTCTC 1 cut(s) 1827
BsrDI GCAATG 2 cut(s) 540, 1663
BsrI ACTGG 3 cut(s) 222, 1553, 1667
BssECI CCNNGG 3 cut(s) 1580, 1837, 1862
BssNI GRCGYC 2 cut(s) 56, 487
BssSI CACGAG 1 cut(s) 947
BssT1I CCWWGG 1 cut(s) 1837
Bst2BI CACGAG 1 cut(s) 947
Bst2UI CCWGG 8 cut(s) 156, 177, 849, 1548, 1581, 1635, 1692, 1864
Bst4CI ACNGT 5 cut(s) 733, 833, 1057, 1343, 1412
Bst6I CTCTTC 2 cut(s) 116, 1686
BstACI GRCGYC 2 cut(s) 56, 487
BstBAI YACGTR 1 cut(s) 413
BstC8I GCNNGC 4 cut(s) 135, 229, 547, 1259
BstDEI CTNAG 5 cut(s) 45, 714, 839, 896, 1586
BstENI CCTNNNNNAGG 1 cut(s) 852
BstF5I GGATG 3 cut(s) 337, 1047, 1885
BstFNI CGCG 2 cut(s) 27, 101
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 1 cut(s) 39
BstMAI GTCTC 7 cut(s) 278, 434, 933, 981, 1022, 1576, 1827
BstNI CCWGG 8 cut(s) 156, 177, 849, 1548, 1581, 1635, 1692, 1864
BstNSI RCATGY 1 cut(s) 1118
BstSCI CCNGG 8 cut(s) 154, 175, 847, 1546, 1579, 1633, 1690, 1862
BstSFI CTRYAG 1 cut(s) 1371
BstUI CGCG 2 cut(s) 27, 101
BstX2I RGATCY 5 cut(s) 4, 48, 559, 1589, 1595
BstYI RGATCY 5 cut(s) 4, 48, 559, 1589, 1595
BsuRI GGCC 5 cut(s) 1199, 1579, 1695, 1725, 1797
BtgZI GCGATG 1 cut(s) 82
BtsCI GGATG 3 cut(s) 337, 1047, 1885
BtsI GCAGTG 3 cut(s) 624, 629, 1614
BtsIMutI CAGTG 7 cut(s) 229, 624, 629, 1062, 1255, 1614, 1674
BtuMI TCGCGA 1 cut(s) 101
Cac8I GCNNGC 4 cut(s) 135, 229, 547, 1259
CaiI CAGNNNCTG 2 cut(s) 52, 790
CciI TCATGA 1 cut(s) 1642
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 5 cut(s) 923, 1207, 1550, 1577, 1724
CseI GACGC 4 cut(s) 64, 203, 476, 1039
Csp6I GTAC 4 cut(s) 29, 729, 811, 1421
CspCI CAANNNNNGTGG 2 cut(s) 1486, 1521
CviAII CATG 7 cut(s) 209, 509, 908, 1073, 1115, 1204, 1643
CviQI GTAC 4 cut(s) 29, 729, 811, 1421
DdeI CTNAG 5 cut(s) 45, 714, 839, 896, 1586
Eam1104I CTCTTC 2 cut(s) 116, 1686
EarI CTCTTC 2 cut(s) 116, 1686
Ecl136II GAGCTC 1 cut(s) 713
Eco130I CCWWGG 1 cut(s) 1837
Eco147I AGGCCT 1 cut(s) 1797
Eco24I GRGCYC 1 cut(s) 715
Eco31I GGTCTC 1 cut(s) 1827
Eco47I GGWCC 3 cut(s) 923, 1207, 1550
Eco47III AGCGCT 1 cut(s) 38
Eco53kI GAGCTC 1 cut(s) 713
Eco57I CTGAAG 4 cut(s) 1067, 1647, 1667, 1812
Eco88I CYCGRG 1 cut(s) 281
EcoICRI GAGCTC 1 cut(s) 713
EcoNI CCTNNNNNAGG 1 cut(s) 852
EcoRI GAATTC 2 cut(s) 254, 1744
EcoRII CCWGG 8 cut(s) 154, 175, 847, 1546, 1579, 1633, 1690, 1862
EcoT14I CCWWGG 1 cut(s) 1837
EcoT38I GRGCYC 1 cut(s) 715
ErhI CCWWGG 1 cut(s) 1837
Esp3I CGTCTC 2 cut(s) 278, 1022
FaeI CATG 7 cut(s) 212, 512, 911, 1076, 1118, 1207, 1646
FalI AAGNNNNNCTT 6 cut(s) 631, 663, 846, 878, 872, 904
FaqI GGGAC 2 cut(s) 263, 1124
FatI CATG 7 cut(s) 208, 508, 907, 1072, 1114, 1203, 1642
FauI CCCGC 1 cut(s) 1292
FbaI TGATCA 2 cut(s) 1186, 1245
FokI GGATG 2 cut(s) 344, 1054
FriOI GRGCYC 1 cut(s) 715
GlaI GCGC 1 cut(s) 38
GsaI CCCAGC 1 cut(s) 1737
GsuI CTGGAG 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 5 cut(s) 1199, 1579, 1695, 1725, 1797
HapII CCGG 2 cut(s) 8, 926
HgaI GACGC 4 cut(s) 64, 203, 476, 1039
HhaI GCGC 1 cut(s) 39
Hin1I GRCGYC 2 cut(s) 56, 487
Hin1II CATG 7 cut(s) 212, 512, 911, 1076, 1118, 1207, 1646
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HindIII AAGCTT 1 cut(s) 1845
HinfI GANTC 7 cut(s) 248, 337, 407, 1036, 1124, 1708, 1856
HpaII CCGG 2 cut(s) 8, 926
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 5 cut(s) 736, 836, 1053, 1232, 1757
Hpy188I TCNGA 8 cut(s) 48, 253, 770, 1041, 1564, 1792, 1823, 1832
Hpy8I GTNNAC 5 cut(s) 736, 836, 1053, 1232, 1757
HpyAV CCTTC 3 cut(s) 1210, 1623, 1787
HpyCH4III ACNGT 5 cut(s) 733, 833, 1057, 1343, 1412
HpyCH4IV ACGT 2 cut(s) 144, 412
HpyF3I CTNAG 5 cut(s) 45, 714, 839, 896, 1586
HpySE526I ACGT 2 cut(s) 144, 412
Hsp92I GRCGYC 2 cut(s) 56, 487
Hsp92II CATG 7 cut(s) 212, 512, 911, 1076, 1118, 1207, 1646
HspAI GCGC 1 cut(s) 37
Kpn2I TCCGGA 1 cut(s) 7
KpnI GGTACC 1 cut(s) 1424
Ksp22I TGATCA 2 cut(s) 1186, 1245
LmnI GCTCC 4 cut(s) 10, 1622, 1636, 1781
LweI GCATC 3 cut(s) 370, 1032, 1396
MaeII ACGT 2 cut(s) 144, 412
MaeIII GTNAC 2 cut(s) 408, 949
MboII GAAGA 3 cut(s) 103, 1464, 1673
MflI RGATCY 5 cut(s) 4, 48, 559, 1589, 1595
MhlI GDGCHC 1 cut(s) 715
MluI ACGCGT 1 cut(s) 25
MlyI GAGTC 3 cut(s) 257, 416, 1030
MmeI TCCRAC 3 cut(s) 505, 1115, 1489
MroI TCCGGA 1 cut(s) 7
MroXI GAANNNNTTC 2 cut(s) 204, 1748
MseI TTAA 5 cut(s) 213, 606, 795, 891, 1532
MslI CAYNNNNRTG 2 cut(s) 263, 1268
MspI CCGG 2 cut(s) 8, 926
MspR9I CCNGG 8 cut(s) 156, 177, 849, 1548, 1581, 1635, 1692, 1864
MvaI CCWGG 8 cut(s) 156, 177, 849, 1548, 1581, 1635, 1692, 1864
MvnI CGCG 2 cut(s) 27, 101
NlaIII CATG 7 cut(s) 212, 512, 911, 1076, 1118, 1207, 1646
NlaIV GGNNCC 6 cut(s) 6, 924, 1409, 1422, 1597, 1689
NmuCI GTSAC 2 cut(s) 408, 949
NruI TCGCGA 1 cut(s) 101
NspI RCATGY 1 cut(s) 1118
OliI CACNNNNGTG 1 cut(s) 263
PagI TCATGA 1 cut(s) 1642
PceI AGGCCT 1 cut(s) 1797
PcsI WCGNNNNNNNCGW 2 cut(s) 141, 1179
PdmI GAANNNNTTC 2 cut(s) 204, 1748
PfeI GAWTC 4 cut(s) 337, 1124, 1708, 1856
PflFI GACNNNGTC 1 cut(s) 1568
PfoI TCCNGGA 1 cut(s) 175
PleI GAGTC 3 cut(s) 256, 415, 1030
PpsI GAGTC 3 cut(s) 256, 415, 1030
Ppu21I YACGTR 1 cut(s) 413
PsiI TTATAA 1 cut(s) 867
Psp124BI GAGCTC 1 cut(s) 715
Psp6I CCWGG 8 cut(s) 154, 175, 847, 1546, 1579, 1633, 1690, 1862
PspFI CCCAGC 1 cut(s) 1733
PspGI CCWGG 8 cut(s) 154, 175, 847, 1546, 1579, 1633, 1690, 1862
PspN4I GGNNCC 6 cut(s) 6, 924, 1409, 1422, 1597, 1689
PspPI GGNCC 5 cut(s) 923, 1207, 1550, 1577, 1724
PstI CTGCAG 1 cut(s) 1375
PstNI CAGNNNCTG 2 cut(s) 52, 790
PsuI RGATCY 5 cut(s) 4, 48, 559, 1589, 1595
PsyI GACNNNGTC 1 cut(s) 1568
RruI TCGCGA 1 cut(s) 101
RsaI GTAC 4 cut(s) 30, 730, 812, 1422
RsaNI GTAC 4 cut(s) 29, 729, 811, 1421
RseI CAYNNNNRTG 2 cut(s) 263, 1268
SacI GAGCTC 1 cut(s) 715
SaqAI TTAA 5 cut(s) 213, 606, 795, 891, 1532
Sau96I GGNCC 5 cut(s) 923, 1207, 1550, 1577, 1724
SchI GAGTC 3 cut(s) 257, 416, 1030
ScrFI CCNGG 8 cut(s) 156, 177, 849, 1548, 1581, 1635, 1692, 1864
SduI GDGCHC 1 cut(s) 715
SfaNI GCATC 3 cut(s) 370, 1032, 1396
SfcI CTRYAG 1 cut(s) 1371
SinI GGWCC 3 cut(s) 923, 1207, 1550
SmiMI CAYNNNNRTG 2 cut(s) 263, 1268
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
SseBI AGGCCT 1 cut(s) 1797
SsiI CCGC 3 cut(s) 383, 914, 1285
SspI AATATT 1 cut(s) 724
SstI GAGCTC 1 cut(s) 715
StuI AGGCCT 1 cut(s) 1797
StyD4I CCNGG 8 cut(s) 154, 175, 847, 1546, 1579, 1633, 1690, 1862
StyI CCWWGG 1 cut(s) 1837
TaaI ACNGT 5 cut(s) 733, 833, 1057, 1343, 1412
TaiI ACGT 2 cut(s) 147, 415
TaqI TCGA 4 cut(s) 592, 754, 1173, 1450
TfiI GAWTC 4 cut(s) 337, 1124, 1708, 1856
Tru1I TTAA 5 cut(s) 213, 606, 795, 891, 1532
Tru9I TTAA 5 cut(s) 213, 606, 795, 891, 1532
TscAI CASTG 7 cut(s) 229, 624, 629, 1062, 1255, 1614, 1674
TseFI GTSAC 2 cut(s) 408, 949
Tsp45I GTSAC 2 cut(s) 408, 949
TspRI CASTG 7 cut(s) 229, 624, 629, 1062, 1255, 1614, 1674
Tth111I GACNNNGTC 1 cut(s) 1568
VpaK11BI GGWCC 3 cut(s) 923, 1207, 1550
XagI CCTNNNNNAGG 1 cut(s) 852
XapI RAATTY 4 cut(s) 254, 290, 311, 1744
XceI RCATGY 1 cut(s) 1118
XmnI GAANNNNTTC 2 cut(s) 204, 1748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.