pycom08g20330

Tumour-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0008622
Physical Location & Seq
Forward (+)
20162332 .. 20165280
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g20330.1

Sequence Viewer

Length: 696 bp
ATGGTCCTCATACTCGTCTGGTTGAGTGAACTATTCACGCTAATCAGTGTTCGGACGCCCATTTCTATGAAGTTCTTTCCCCGCTTCTTTTTGATCTACTTTCTTGTTTTCCACATCTATTTCTTTTCCTATAGTTATGGTTTTTCTTATTTGGCTCTTTCTACAGCTGCCGCTTTTATGCAGCATCTCATTCTATACTTCTGGAACCGTTTTGAGGTACCAACTTTACAGAGGTTCATCCAAAATCGACGATCTCAGCTCCAGCAACACCCAGATTTCCACATTACCTCGTCAACGTTCCTTGCCTCAACGGTACAGATCACAAGATTGAACCCAAGAAACCCCGACCTAGTTAATTCTGACTTGGAGCCTGGACCCGGGCTGAGATCTGGATCAAATCCAGCAGTACCATCAAATGCAGCAGAAGCACCCGGCCTTCAAGAACAGTCAGGTAACGACAACCTAGACGGGGTAGGAAACCCTCAGGCTCCCGGCCAAGCTGATCTGAATCAAGCAGAGAATGGTCCCAACCCCGGCGCTATGAATTCATTCAGTTCGCTTCTGTTATGGATCTTAGGAGGGGCGTCCTCTGAAGGCGGCCTCAATTCCCTTCTTTCTATGTTCAGAGATGTGAGAGAGCAAGGACAAGTTTACACGGAAGCCCCTAGGCCAGAAAATCAAGACATACAAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Membralin

Protein Analysis

232

Amino Acids

25.86

Weight (kDa)

5.52

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 217
AccB1I GGYRCC 1 cut(s) 217
AciI CCGC 3 cut(s) 82, 171, 597
AclI AACGTT 1 cut(s) 296
AclWI GGATC 2 cut(s) 400, 578
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 1 cut(s) 544
AcuI CTGAAG 1 cut(s) 612
AcyI GRCGYC 2 cut(s) 56, 584
AfaI GTAC 3 cut(s) 219, 315, 408
AfiI CCNNNNNNNGG 4 cut(s) 214, 377, 469, 533
AgsI TTSAA 2 cut(s) 331, 440
AjnI CCWGG 1 cut(s) 370
AluBI AGCT 3 cut(s) 167, 259, 500
AluI AGCT 3 cut(s) 167, 259, 500
AlwI GGATC 2 cut(s) 400, 578
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 4 cut(s) 433, 493, 598, 668
ApeKI GCWGC 3 cut(s) 167, 181, 419
ApoI RAATTY 1 cut(s) 544
Asp700I GAANNNNTTC 1 cut(s) 548
Asp718I GGTACC 1 cut(s) 217
AspA2I CCTAGG 1 cut(s) 665
AspLEI GCGC 1 cut(s) 539
AspS9I GGNCC 3 cut(s) 4, 374, 524
AsuC2I CCSGG 5 cut(s) 378, 379, 432, 492, 534
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 3 cut(s) 4, 374, 524
AvrII CCTAGG 1 cut(s) 665
AxyI CCTNAGG 1 cut(s) 483
BanI GGYRCC 1 cut(s) 217
BbvI GCAGC 3 cut(s) 154, 193, 431
BccI CCATC 1 cut(s) 418
BciT130I CCWGG 1 cut(s) 372
BcnI CCSGG 5 cut(s) 378, 379, 432, 492, 534
BfaI CTAG 3 cut(s) 350, 464, 666
BfmI CTRYAG 2 cut(s) 130, 162
BfoI RGCGCY 1 cut(s) 540
BglII AGATCT 1 cut(s) 386
BisI GCNGC 5 cut(s) 168, 171, 182, 420, 598
BlnI CCTAGG 1 cut(s) 665
BlsI GCNGC 5 cut(s) 169, 172, 183, 421, 599
Bme1390I CCNGG 6 cut(s) 372, 378, 379, 432, 492, 534
Bme18I GGWCC 3 cut(s) 4, 374, 524
BmeT110I CYCGRG 1 cut(s) 377
BmgT120I GGNCC 3 cut(s) 4, 374, 524
BmiI GGNNCC 6 cut(s) 206, 219, 369, 376, 489, 526
BmrFI CCNGG 6 cut(s) 372, 378, 379, 432, 492, 534
BmsI GCATC 1 cut(s) 193
BpmI CTGGAG 1 cut(s) 245
BpuMI CCSGG 5 cut(s) 378, 379, 432, 492, 534
BsaBI GATNNNNATC 2 cut(s) 391, 507
BsaHI GRCGYC 2 cut(s) 56, 584
BsaJI CCNNGG 3 cut(s) 377, 532, 665
Bsc4I CCNNNNNNNGG 4 cut(s) 214, 377, 469, 533
Bse21I CCTNAGG 1 cut(s) 483
Bse8I GATNNNNATC 2 cut(s) 391, 507
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 3 cut(s) 377, 532, 665
BseGI GGATG 1 cut(s) 237
BseJI GATNNNNATC 2 cut(s) 391, 507
BseLI CCNNNNNNNGG 4 cut(s) 214, 377, 469, 533
BseMII CTCAG 3 cut(s) 269, 374, 497
BseXI GCAGC 3 cut(s) 154, 193, 431
BshFI GGCC 4 cut(s) 435, 495, 600, 670
BshNI GGYRCC 1 cut(s) 217
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 4 cut(s) 378, 432, 492, 534
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 4 cut(s) 214, 377, 469, 533
BsmFI GGGAC 1 cut(s) 510
BsnI GGCC 4 cut(s) 435, 495, 600, 670
BsoBI CYCGRG 1 cut(s) 377
Bsp143I GATC 7 cut(s) 93, 251, 318, 386, 392, 502, 570
BspACI CCGC 3 cut(s) 82, 171, 597
BspANI GGCC 4 cut(s) 435, 495, 600, 670
BspCNI CTCAG 3 cut(s) 268, 375, 496
BspLI GGNNCC 6 cut(s) 206, 219, 369, 376, 489, 526
BspPI GGATC 2 cut(s) 400, 578
BspT107I GGYRCC 1 cut(s) 217
BssECI CCNNGG 3 cut(s) 377, 532, 665
BssMI GATC 7 cut(s) 93, 251, 318, 386, 392, 502, 570
BssNI GRCGYC 2 cut(s) 56, 584
BssT1I CCWWGG 1 cut(s) 665
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 3 cut(s) 209, 313, 447
BstACI GRCGYC 2 cut(s) 56, 584
BstDEI CTNAG 4 cut(s) 255, 383, 483, 574
BstF5I GGATG 1 cut(s) 237
BstH2I RGCGCY 1 cut(s) 540
BstHHI GCGC 1 cut(s) 539
BstKTI GATC 7 cut(s) 96, 254, 321, 389, 395, 505, 573
BstMBI GATC 7 cut(s) 93, 251, 318, 386, 392, 502, 570
BstMWI GCNNNNNNNGC 1 cut(s) 425
BstNI CCWGG 1 cut(s) 372
BstSCI CCNGG 6 cut(s) 370, 376, 377, 430, 490, 532
BstSFI CTRYAG 2 cut(s) 130, 162
BstV1I GCAGC 3 cut(s) 154, 193, 431
BstX2I RGATCY 2 cut(s) 386, 570
BstYI RGATCY 2 cut(s) 386, 570
Bsu36I CCTNAGG 1 cut(s) 483
BsuRI GGCC 4 cut(s) 435, 495, 600, 670
BtsCI GGATG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 52
CfoI GCGC 1 cut(s) 539
Cfr13I GGNCC 3 cut(s) 4, 374, 524
Cfr9I CCCGGG 1 cut(s) 377
CseI GACGC 2 cut(s) 64, 573
Csp6I GTAC 3 cut(s) 218, 314, 407
CviQI GTAC 3 cut(s) 218, 314, 407
DdeI CTNAG 4 cut(s) 255, 383, 483, 574
DpnI GATC 7 cut(s) 95, 253, 320, 388, 394, 504, 572
DpnII GATC 7 cut(s) 93, 251, 318, 386, 392, 502, 570
EaeI YGGCCR 1 cut(s) 493
Eco130I CCWWGG 1 cut(s) 665
Eco47I GGWCC 3 cut(s) 4, 374, 524
Eco57I CTGAAG 1 cut(s) 612
Eco81I CCTNAGG 1 cut(s) 483
Eco88I CYCGRG 1 cut(s) 377
EcoRI GAATTC 1 cut(s) 544
EcoRII CCWGG 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 665
ErhI CCWWGG 1 cut(s) 665
FaqI GGGAC 1 cut(s) 510
FauI CCCGC 1 cut(s) 89
Fnu4HI GCNGC 5 cut(s) 168, 171, 182, 420, 598
FokI GGATG 1 cut(s) 224
Fsp4HI GCNGC 5 cut(s) 168, 171, 182, 420, 598
FspBI CTAG 3 cut(s) 350, 464, 666
GlaI GCGC 1 cut(s) 538
GluI GCNGC 5 cut(s) 168, 171, 182, 420, 598
GsuI CTGGAG 1 cut(s) 245
HaeII RGCGCY 1 cut(s) 540
HaeIII GGCC 4 cut(s) 435, 495, 600, 670
HapII CCGG 4 cut(s) 378, 432, 492, 534
HgaI GACGC 2 cut(s) 64, 573
HhaI GCGC 1 cut(s) 539
Hin1I GRCGYC 2 cut(s) 56, 584
Hin6I GCGC 1 cut(s) 537
HinP1I GCGC 1 cut(s) 537
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HinfI GANTC 1 cut(s) 508
HpaII CCGG 4 cut(s) 378, 432, 492, 534
Hpy166II GTNNAC 3 cut(s) 29, 294, 652
Hpy188I TCNGA 5 cut(s) 54, 361, 507, 592, 626
Hpy188III TCNNGA 4 cut(s) 202, 390, 440, 680
Hpy8I GTNNAC 3 cut(s) 29, 294, 652
Hpy99I CGWCG 1 cut(s) 252
HpyAV CCTTC 3 cut(s) 446, 587, 620
HpyCH4III ACNGT 3 cut(s) 209, 313, 447
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 2 cut(s) 181, 419
HpyF10VI GCNNNNNNNGC 1 cut(s) 425
HpyF3I CTNAG 4 cut(s) 255, 383, 483, 574
HpySE526I ACGT 1 cut(s) 296
Hsp92I GRCGYC 2 cut(s) 56, 584
HspAI GCGC 1 cut(s) 537
KpnI GGTACC 1 cut(s) 221
Kzo9I GATC 7 cut(s) 93, 251, 318, 386, 392, 502, 570
LmnI GCTCC 3 cut(s) 264, 367, 493
Lsp1109I GCAGC 3 cut(s) 154, 193, 431
LweI GCATC 1 cut(s) 193
MaeI CTAG 3 cut(s) 350, 464, 666
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 1 cut(s) 452
MalI GATC 7 cut(s) 95, 253, 320, 388, 394, 504, 572
MboI GATC 7 cut(s) 93, 251, 318, 386, 392, 502, 570
MflI RGATCY 2 cut(s) 386, 570
MluCI AATT 3 cut(s) 355, 544, 604
MnlI CCTC 9 cut(s) 17, 208, 225, 298, 316, 492, 572, 598, 611
MroXI GAANNNNTTC 1 cut(s) 548
MseI TTAA 1 cut(s) 354
MslI CAYNNNNRTG 1 cut(s) 65
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 4 cut(s) 378, 432, 492, 534
MspR9I CCNGG 6 cut(s) 372, 378, 379, 432, 492, 534
MvaI CCWGG 1 cut(s) 372
MwoI GCNNNNNNNGC 1 cut(s) 425
NciI CCSGG 5 cut(s) 378, 379, 432, 492, 534
NdeII GATC 7 cut(s) 93, 251, 318, 386, 392, 502, 570
NlaIV GGNNCC 6 cut(s) 206, 219, 369, 376, 489, 526
PdmI GAANNNNTTC 1 cut(s) 548
PfeI GAWTC 1 cut(s) 508
PkrI GCNGC 5 cut(s) 169, 172, 183, 421, 599
Psp1406I AACGTT 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 6 cut(s) 206, 219, 369, 376, 489, 526
PspPI GGNCC 3 cut(s) 4, 374, 524
PsuI RGATCY 2 cut(s) 386, 570
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 3 cut(s) 219, 315, 408
RsaNI GTAC 3 cut(s) 218, 314, 407
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 5 cut(s) 168, 171, 182, 420, 598
Sau3AI GATC 7 cut(s) 93, 251, 318, 386, 392, 502, 570
Sau96I GGNCC 3 cut(s) 4, 374, 524
ScrFI CCNGG 6 cut(s) 372, 378, 379, 432, 492, 534
SfaNI GCATC 1 cut(s) 193
SfcI CTRYAG 2 cut(s) 130, 162
SinI GGWCC 3 cut(s) 4, 374, 524
SmaI CCCGGG 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 65
Sse9I AATT 3 cut(s) 355, 544, 604
SsiI CCGC 3 cut(s) 82, 171, 597
SspMI CTAG 3 cut(s) 350, 464, 666
StyD4I CCNGG 6 cut(s) 370, 376, 377, 430, 490, 532
StyI CCWWGG 1 cut(s) 665
TaaI ACNGT 3 cut(s) 209, 313, 447
TaiI ACGT 1 cut(s) 299
TaqI TCGA 1 cut(s) 247
TasI AATT 3 cut(s) 355, 544, 604
TauI GCSGC 2 cut(s) 173, 600
TfiI GAWTC 1 cut(s) 508
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 1 cut(s) 52
TseI GCWGC 3 cut(s) 167, 181, 419
TspDTI ATGAA 4 cut(s) 83, 226, 537, 557
TspGWI ACGGA 1 cut(s) 671
TspMI CCCGGG 1 cut(s) 377
TspRI CASTG 1 cut(s) 52
VpaK11BI GGWCC 3 cut(s) 4, 374, 524
XapI RAATTY 1 cut(s) 544
XmaI CCCGGG 1 cut(s) 377
XmaJI CCTAGG 1 cut(s) 665
XmnI GAANNNNTTC 1 cut(s) 548
XspI CTAG 3 cut(s) 350, 464, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.