RLG00000029682

Tumour-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008622
Physical Location & Seq
Reverse (-)
42923639 .. 42944217
20579 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029682

Sequence Viewer

Length: 1224 bp
ATGTGGTTGGGGTTGTTGGAGGAAGTGAGATTGAGTTGGGAAGTTTCATCTGCTTTAATTTTTCATTTTGAGAAACTGAAGGGTTATTTCTTACTGCCTGAAAGTGCAAGAGCTCAGCCTCAGCATAATATTCGTACTGTAAACATAAGCATATCAGCTCGACATTCCTGTTTCGGAAACAGGTGGCAGCAGCTACTGATTAACAGATTTGTTGGGTACGATACTATTTTGACAAACAGTTTACAGAGTTCTCCTGATCAAGGTTATCTTTATAATTTTCAAACAAAGGAGTTCTTTAATCTTAGTTATGCACATGAACCGCCAGAGGGTCCGGCAAGATTTGGAGACTACCTCGTGACAAAGTGTGGCGTTCTTATGATGTCTCTATTTGTGTTCTTTACAACCACTATGTCTGTGTCATTTACTTTGAGAGAGAAGCAGACTCGGATGCCGAAGTTCACAGTGCAGCTTCAACACCATGCTCGTCATCGGCTTCCAACATTTCAGTTGATTTTTGTGCATGTAATTGAATCACTTGTCTTTGTCCCGATTATGATTGGCATATTGTTTTTTCTGTTCGAGTTTTACGATGATCAACTATTGGCTTTCATGGTCCTAATTCTTGTCTGGTTGAGTGAACTATTTACCCTGATCAGTGTTCGCACGCCCATATCAATGAAGTTCTTTCCCCGCTTCTTTCTTCTCTACTTTCTGGTTTTTCACATCTATTTCTTTTCCTATGCTTACGGAGTTACGGTATGTTTTTCCTATTTGGCTCTTTCTACGGCTGCAGCGTTTATGCAGCATCTCATTCTATACTTCTGGAACCGTTTTGAGGTACCAACTCTACAAAGGTTTATTCAAAATCGAAGATCACAACTCCAACAACACCCAGATTTCCACATTACTTCCTCCACATTCCTTGCCTCAACTTTGCATATCACAAGATTAAACACAAGAAATCCTGGTCCAGTCAATTCTGCAGATATAGTCTCTGGGCCTGGGCTGAGATCTGGATCCAATCCAGCAGTGCCTTCAAATGGAGCTGAAGCTCCTGGCTTTCATGAACGATCAGGCAATGAGAACCCAGTGCAAAACCCTCTTCAGGAGGAGCTAGCTTCCTCTGAAGGCCTCAACTCATTTCTTTCTATGTTCAGAGATGTCCGAGACCAAGGACAAGCTTATGCTGAATCTCCCAGGCAAGAAAATCAGGATGGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Membralin

Protein Analysis

408

Amino Acids

47.02

Weight (kDa)

7.83

Isoelectric Point (pI)

54.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Membralin PF09746 27 - 226 2.2e-13 Tumour-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 273
Acc65I GGTACC 1 cut(s) 838
AccB1I GGYRCC 1 cut(s) 838
AciI CCGC 2 cut(s) 320, 691
AclWI GGATC 2 cut(s) 1011, 1024
AcuI CTGAAG 4 cut(s) 98, 1068, 1088, 1146
AfaI GTAC 3 cut(s) 136, 218, 840
AfiI CCNNNNNNNGG 5 cut(s) 260, 326, 835, 1040, 1105
AgsI TTSAA 5 cut(s) 281, 473, 530, 863, 1038
AjnI CCWGG 4 cut(s) 964, 1000, 1054, 1196
AluBI AGCT 9 cut(s) 113, 158, 193, 469, 1046, 1052, 1114, 1118, 1181
AluI AGCT 9 cut(s) 113, 158, 193, 469, 1046, 1052, 1114, 1118, 1181
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 4 cut(s) 339, 387, 997, 1161
AlwI GGATC 2 cut(s) 1011, 1024
AlwNI CAGNNNCTG 1 cut(s) 196
AoxI GGCC 2 cut(s) 998, 1129
ApeKI GCWGC 6 cut(s) 187, 190, 466, 788, 791, 802
Asp718I GGTACC 1 cut(s) 838
AspS9I GGNCC 4 cut(s) 329, 613, 968, 998
AsuNHI GCTAGC 1 cut(s) 1114
AvaII GGWCC 3 cut(s) 329, 613, 968
BamHI GGATCC 1 cut(s) 1016
BanI GGYRCC 1 cut(s) 838
BanII GRGCYC 1 cut(s) 115
BauI CACGAG 1 cut(s) 353
Bbv12I GWGCWC 1 cut(s) 115
BbvCI CCTCAGC 1 cut(s) 120
BbvI GCAGC 6 cut(s) 199, 202, 478, 775, 803, 814
BccI CCATC 1 cut(s) 1208
BceAI ACGGC 1 cut(s) 801
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 4 cut(s) 966, 1002, 1056, 1198
BclI TGATCA 3 cut(s) 256, 592, 651
BcoDI GTCTC 4 cut(s) 339, 387, 997, 1161
BfaI CTAG 1 cut(s) 1115
BfmI CTRYAG 2 cut(s) 789, 981
BglII AGATCT 1 cut(s) 1010
BisI GCNGC 6 cut(s) 188, 191, 467, 789, 792, 803
BlpI GCTNAGC 1 cut(s) 114
BlsI GCNGC 6 cut(s) 189, 192, 468, 790, 793, 804
Bme1390I CCNGG 4 cut(s) 966, 1002, 1056, 1198
Bme18I GGWCC 3 cut(s) 329, 613, 968
BmgT120I GGNCC 4 cut(s) 329, 613, 968, 998
BmiI GGNNCC 4 cut(s) 330, 827, 840, 1018
BmrFI CCNGG 4 cut(s) 966, 1002, 1056, 1198
BmrI ACTGGG 1 cut(s) 1082
BmsI GCATC 2 cut(s) 438, 814
BmtI GCTAGC 1 cut(s) 1118
BmuI ACTGGG 1 cut(s) 1082
BplI GAGNNNNNCTC 2 cut(s) 336, 368
Bpu10I CCTNAGC 1 cut(s) 120
Bpu1102I GCTNAGC 1 cut(s) 114
BsaBI GATNNNNATC 1 cut(s) 1015
BsaI GGTCTC 1 cut(s) 1161
BsaJI CCNNGG 3 cut(s) 1001, 1171, 1196
BsaXI ACNNNNNCTCC 2 cut(s) 741, 771
Bsc4I CCNNNNNNNGG 5 cut(s) 260, 326, 835, 1040, 1105
Bse1I ACTGG 2 cut(s) 971, 1088
Bse3DI GCAATG 1 cut(s) 1084
Bse8I GATNNNNATC 1 cut(s) 1015
BseBI CCWGG 4 cut(s) 966, 1002, 1056, 1198
BseDI CCNNGG 3 cut(s) 1001, 1171, 1196
BseGI GGATG 2 cut(s) 453, 1219
BseJI GATNNNNATC 1 cut(s) 1015
BseLI CCNNNNNNNGG 5 cut(s) 260, 326, 835, 1040, 1105
BseMI GCAATG 1 cut(s) 1084
BseMII CTCAG 3 cut(s) 128, 134, 998
BseNI ACTGG 2 cut(s) 971, 1088
BseRI GAGGAG 1 cut(s) 1124
BseXI GCAGC 6 cut(s) 199, 202, 478, 775, 803, 814
BsgI GTGCAG 1 cut(s) 485
BshFI GGCC 2 cut(s) 1000, 1131
BshNI GGYRCC 1 cut(s) 838
BsiHKAI GWGCWC 1 cut(s) 115
BsiSI CCGG 1 cut(s) 332
BslFI GGGAC 1 cut(s) 530
BslI CCNNNNNNNGG 5 cut(s) 260, 326, 835, 1040, 1105
BsmAI GTCTC 4 cut(s) 339, 387, 997, 1161
BsmFI GGGAC 1 cut(s) 530
BsnI GGCC 2 cut(s) 1000, 1131
Bso31I GGTCTC 1 cut(s) 1161
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 7 cut(s) 256, 592, 651, 872, 1010, 1016, 1070
Bsp1720I GCTNAGC 1 cut(s) 114
BspACI CCGC 2 cut(s) 320, 691
BspANI GGCC 2 cut(s) 1000, 1131
BspCNI CTCAG 3 cut(s) 127, 133, 999
BspHI TCATGA 1 cut(s) 1063
BspLI GGNNCC 4 cut(s) 330, 827, 840, 1018
BspMAI CTGCAG 2 cut(s) 793, 985
BspOI GCTAGC 1 cut(s) 1118
BspPI GGATC 2 cut(s) 1011, 1024
BspT107I GGYRCC 1 cut(s) 838
BspTNI GGTCTC 1 cut(s) 1161
BsrDI GCAATG 1 cut(s) 1084
BsrI ACTGG 2 cut(s) 971, 1088
BssECI CCNNGG 3 cut(s) 1001, 1171, 1196
BssMI GATC 7 cut(s) 256, 592, 651, 872, 1010, 1016, 1070
BssSI CACGAG 1 cut(s) 353
BssT1I CCWWGG 1 cut(s) 1171
Bst2BI CACGAG 1 cut(s) 353
Bst2UI CCWGG 4 cut(s) 966, 1002, 1056, 1198
Bst4CI ACNGT 5 cut(s) 139, 239, 463, 757, 830
Bst6I CTCTTC 1 cut(s) 1107
BstC8I GCNNGC 2 cut(s) 665, 1116
BstDEI CTNAG 4 cut(s) 114, 120, 302, 1007
BstENI CCTNNNNNAGG 1 cut(s) 258
BstF5I GGATG 2 cut(s) 453, 1219
BstKTI GATC 7 cut(s) 259, 595, 654, 875, 1013, 1019, 1073
BstMAI GTCTC 4 cut(s) 339, 387, 997, 1161
BstMBI GATC 7 cut(s) 256, 592, 651, 872, 1010, 1016, 1070
BstNI CCWGG 4 cut(s) 966, 1002, 1056, 1198
BstNSI RCATGY 1 cut(s) 524
BstSCI CCNGG 4 cut(s) 964, 1000, 1054, 1196
BstSFI CTRYAG 2 cut(s) 789, 981
BstV1I GCAGC 6 cut(s) 199, 202, 478, 775, 803, 814
BstX2I RGATCY 2 cut(s) 1010, 1016
BstYI RGATCY 2 cut(s) 1010, 1016
BsuRI GGCC 2 cut(s) 1000, 1131
BtsCI GGATG 2 cut(s) 453, 1219
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 4 cut(s) 468, 661, 1035, 1095
Cac8I GCNNGC 2 cut(s) 665, 1116
CaiI CAGNNNCTG 1 cut(s) 196
CciI TCATGA 1 cut(s) 1063
Cfr13I GGNCC 4 cut(s) 329, 613, 968, 998
Csp6I GTAC 3 cut(s) 135, 217, 839
CspCI CAANNNNNGTGG 2 cut(s) 904, 939
CviAII CATG 5 cut(s) 314, 479, 521, 610, 1064
CviQI GTAC 3 cut(s) 135, 217, 839
DdeI CTNAG 4 cut(s) 114, 120, 302, 1007
DpnI GATC 7 cut(s) 258, 594, 653, 874, 1012, 1018, 1072
DpnII GATC 7 cut(s) 256, 592, 651, 872, 1010, 1016, 1070
Eam1104I CTCTTC 1 cut(s) 1107
EarI CTCTTC 1 cut(s) 1107
Ecl136II GAGCTC 1 cut(s) 113
Eco130I CCWWGG 1 cut(s) 1171
Eco147I AGGCCT 1 cut(s) 1131
Eco24I GRGCYC 1 cut(s) 115
Eco31I GGTCTC 1 cut(s) 1161
Eco47I GGWCC 3 cut(s) 329, 613, 968
Eco53kI GAGCTC 1 cut(s) 113
Eco57I CTGAAG 4 cut(s) 98, 1068, 1088, 1146
EcoICRI GAGCTC 1 cut(s) 113
EcoNI CCTNNNNNAGG 1 cut(s) 258
EcoRII CCWGG 4 cut(s) 964, 1000, 1054, 1196
EcoT14I CCWWGG 1 cut(s) 1171
EcoT38I GRGCYC 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 1171
FaeI CATG 5 cut(s) 317, 482, 524, 613, 1067
FalI AAGNNNNNCTT 4 cut(s) 252, 284, 278, 310
FaqI GGGAC 1 cut(s) 530
FatI CATG 5 cut(s) 313, 478, 520, 609, 1063
FauI CCCGC 1 cut(s) 698
FbaI TGATCA 3 cut(s) 256, 592, 651
Fnu4HI GCNGC 6 cut(s) 188, 191, 467, 789, 792, 803
FokI GGATG 1 cut(s) 460
FriOI GRGCYC 1 cut(s) 115
Fsp4HI GCNGC 6 cut(s) 188, 191, 467, 789, 792, 803
FspBI CTAG 1 cut(s) 1115
GluI GCNGC 6 cut(s) 188, 191, 467, 789, 792, 803
HaeIII GGCC 2 cut(s) 1000, 1131
HapII CCGG 1 cut(s) 332
Hin1II CATG 5 cut(s) 317, 482, 524, 613, 1067
HindIII AAGCTT 1 cut(s) 1179
HinfI GANTC 3 cut(s) 442, 530, 1190
HpaII CCGG 1 cut(s) 332
Hpy166II GTNNAC 4 cut(s) 142, 242, 459, 638
Hpy188I TCNGA 5 cut(s) 176, 447, 1126, 1157, 1166
Hpy188III TCNNGA 8 cut(s) 254, 355, 547, 823, 1014, 1064, 1106, 1211
Hpy8I GTNNAC 4 cut(s) 142, 242, 459, 638
HpyAV CCTTC 3 cut(s) 73, 1044, 1121
HpyCH4III ACNGT 5 cut(s) 139, 239, 463, 757, 830
HpyCH4V TGCA 9 cut(s) 107, 311, 466, 520, 791, 802, 937, 983, 1093
HpyF3I CTNAG 4 cut(s) 114, 120, 302, 1007
Hsp92II CATG 5 cut(s) 317, 482, 524, 613, 1067
KpnI GGTACC 1 cut(s) 842
Ksp22I TGATCA 3 cut(s) 256, 592, 651
Kzo9I GATC 7 cut(s) 256, 592, 651, 872, 1010, 1016, 1070
LmnI GCTCC 3 cut(s) 1043, 1057, 1111
Lsp1109I GCAGC 6 cut(s) 199, 202, 478, 775, 803, 814
LweI GCATC 2 cut(s) 438, 814
MaeI CTAG 1 cut(s) 1115
MaeIII GTNAC 2 cut(s) 355, 751
MalI GATC 7 cut(s) 258, 594, 653, 874, 1012, 1018, 1072
MboI GATC 7 cut(s) 256, 592, 651, 872, 1010, 1016, 1070
MboII GAAGA 3 cut(s) 692, 882, 1094
MflI RGATCY 2 cut(s) 1010, 1016
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 5 cut(s) 57, 274, 525, 618, 976
MlyI GAGTC 1 cut(s) 436
MmeI TCCRAC 2 cut(s) 521, 907
MseI TTAA 4 cut(s) 56, 201, 297, 950
MslI CAYNNNNRTG 1 cut(s) 674
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 4 cut(s) 966, 1002, 1056, 1198
MvaI CCWGG 4 cut(s) 966, 1002, 1056, 1198
NdeII GATC 7 cut(s) 256, 592, 651, 872, 1010, 1016, 1070
NheI GCTAGC 1 cut(s) 1114
NlaIII CATG 5 cut(s) 317, 482, 524, 613, 1067
NlaIV GGNNCC 4 cut(s) 330, 827, 840, 1018
NmuCI GTSAC 1 cut(s) 355
NspI RCATGY 1 cut(s) 524
PagI TCATGA 1 cut(s) 1063
PceI AGGCCT 1 cut(s) 1131
PcsI WCGNNNNNNNCGW 2 cut(s) 585, 791
PfeI GAWTC 2 cut(s) 530, 1190
PkrI GCNGC 6 cut(s) 189, 192, 468, 790, 793, 804
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
PsiI TTATAA 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 115
Psp6I CCWGG 4 cut(s) 964, 1000, 1054, 1196
PspGI CCWGG 4 cut(s) 964, 1000, 1054, 1196
PspN4I GGNNCC 4 cut(s) 330, 827, 840, 1018
PspPI GGNCC 4 cut(s) 329, 613, 968, 998
PstI CTGCAG 2 cut(s) 793, 985
PstNI CAGNNNCTG 1 cut(s) 196
PsuI RGATCY 2 cut(s) 1010, 1016
RsaI GTAC 3 cut(s) 136, 218, 840
RsaNI GTAC 3 cut(s) 135, 217, 839
RseI CAYNNNNRTG 1 cut(s) 674
SacI GAGCTC 1 cut(s) 115
SaqAI TTAA 4 cut(s) 56, 201, 297, 950
SatI GCNGC 6 cut(s) 188, 191, 467, 789, 792, 803
Sau3AI GATC 7 cut(s) 256, 592, 651, 872, 1010, 1016, 1070
Sau96I GGNCC 4 cut(s) 329, 613, 968, 998
SchI GAGTC 1 cut(s) 436
ScrFI CCNGG 4 cut(s) 966, 1002, 1056, 1198
SduI GDGCHC 1 cut(s) 115
SfaNI GCATC 2 cut(s) 438, 814
SfcI CTRYAG 2 cut(s) 789, 981
SinI GGWCC 3 cut(s) 329, 613, 968
SmiMI CAYNNNNRTG 1 cut(s) 674
Sse9I AATT 5 cut(s) 57, 274, 525, 618, 976
SseBI AGGCCT 1 cut(s) 1131
SsiI CCGC 2 cut(s) 320, 691
SspI AATATT 1 cut(s) 130
SspMI CTAG 1 cut(s) 1115
SstI GAGCTC 1 cut(s) 115
StuI AGGCCT 1 cut(s) 1131
StyD4I CCNGG 4 cut(s) 964, 1000, 1054, 1196
StyI CCWWGG 1 cut(s) 1171
TaaI ACNGT 5 cut(s) 139, 239, 463, 757, 830
TaqI TCGA 3 cut(s) 160, 579, 868
TasI AATT 5 cut(s) 57, 274, 525, 618, 976
TfiI GAWTC 2 cut(s) 530, 1190
Tru1I TTAA 4 cut(s) 56, 201, 297, 950
Tru9I TTAA 4 cut(s) 56, 201, 297, 950
TscAI CASTG 4 cut(s) 468, 661, 1035, 1095
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 6 cut(s) 187, 190, 466, 788, 791, 802
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 7 cut(s) 36, 53, 330, 598, 692, 1052, 1080
TspGWI ACGGA 1 cut(s) 762
TspRI CASTG 4 cut(s) 468, 661, 1035, 1095
VpaK11BI GGWCC 3 cut(s) 329, 613, 968
XagI CCTNNNNNAGG 1 cut(s) 258
XceI RCATGY 1 cut(s) 524
XspI CTAG 1 cut(s) 1115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.