Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0003669

Physical Location & Seq

Genomic Coordinates Forward (+)

20867334 .. 20874615

Gene Structure 7282 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g21370.7

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1803 bp

ATGTCTTCTTCATCGTCCTCGTCGTCGAGGCGAGTGGTGAAGCGTGCACAATACAAGGATGCGGCAAAATTAAAGAACCCTGGCATTCCTGGCGTTTTAACTATGGTTAAGGACAAGTTTGTGTTCAAGCCAAACGATCCCACGTCTACCCAAACCCTTGATGTGGAGTTTAGGCATATTAAAGGTCACAAAAACACCAAGGAGGGATCCAAGCAAGCACCTTTGCTTAATCTCAGCAGCAGTCGTGAGGTTAAAAGTTACATTTTTGAGTTTAAAAGTTTTCCCGATCTTCATGAATGCCGGGATTTTGTAGGCAATGCCCTATCTAAGGCTGCAGAAGCTGCAAAAACTGCTCCTAAGAAAGATGGAGTTACATTGCCTGATGAACAACTCAGTGCAGCAGAAATGGAGCTCCGTATGAAGCTATTGCAAGAAGACAGTGAACTGCAGAACCTTCATATGCAGTTTGTAATCAGTGGTGTTTTGACAGAATCTGAGTTCTGGGCAACTAGGAAGAAGTTGCTTGATGGAGATAGCCGTACAAAGCCAAAACAACGGGTTGGTTTTAAAAATTCAATGATTTTGGACACCAAACCTACAAGTGATGGTCGGATGAACAAGGTTACGTTTAATTTCACTCCAGAGATTAAATATCAGATCTTTGCTCTCAAACCAGCTGTTCACCAGGCATTTCTTGAACTAGTGCCCAGTAAGATGACAGCAAAAGACTTCTGGACAAAATATTTCAGAGCTGAATTCCTTCACAGTACCAAGAATACTGCTGCAATTGCGGCAGAAGCTGCTGAGGATGAGGAACTGGCAATTTTTTTAAAGGAGGATGAAATATTGGCAAGGGAAGCTCGTCAGAAGATTCGACGGGTTGATCCAACTTTGGATATGGAAGCTGACCAAGGGGATGATTATACACATCTTCCTGATCATGGGATCTTTCGTGATGGTGGCAAGGACATCACTGAACTACAGAATGAGCAATACAGGAGGACTTTATCACAAGACCTTAACAGGCAAGGGGCTGTTGTTCTTCAAGGAAGAACTGTTGATGGGGATTTAGAAGATCCAAATAATGTTGCAGAAGCACTTATGCGGTCAAGACAGGAGCCTGATGAGAGTGCTGTGCAGGAGAGACTTGATAGGATTGCTCGGATGAATGAGATAGATGACCTTCGAGAACCTCACGATCATCCTGTTGCTCAATTATGTATTAAGGATCCTCGAGACTACTTTGACACGCAACAAGCTAATGCACTTAAAGCTTTGGATGAATCACGAATAGGAACTGAACAAAAAAAATGCAGCATGACCACTGAGGAAGCATATGGCTCCTTGAAGGAGTCTATATCTAAAATTAAGAGCATTGGATTGAAAAATTCCACAGTTGCACCAGAAATTGCTCTTATGGTTCTTAATGGACTGACTCAGAATATTTCTAGTACCAAGTATCAACTCGGAAAGAATCCTCGGGAGGGTATTCTGGACAGTTTGCCAAACAAAATGAAAGAGGAACTGCTGCATCATTCGATGTCCATTCAAGAATTACTGAGGCATTTTTGGTCTACATATCCAATTACCACAGCTTATCTTTCTACAAAGGTAGGCAAACTGAAGGATGCCATGTCACAAATCTATCGACAGCTAGAGGAGATAAAGGAATCAGACTTCCGGCACCAGGTTTCTCTCCTTGTTCGGCCAATGCATCAGGCACTTGATGCGGCTTTTCAACATTATGAATCGGACTTACAGAAAAGAGCTGCAACTGCAACGATGCCGAATGGAAATGCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000439 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005675 GO:0006139 GO:0006259 GO:0006281 GO:0006351 GO:0006355 GO:0006357 GO:0006360 GO:0006366 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016310 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0030880 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032806 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070816 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097659 GO:1901360 GO:1901362 GO:1901564 GO:1901576 GO:1902494 GO:1902554 GO:1902911 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03022 ko03420 ko05203 map03022 map03420 map05203

Pfam Domains

Protein Families

BSD PH_TFIIH

Protein Analysis

601

Amino Acids

67.95

Weight (kDa)

6.63

Isoelectric Point (pI)

42.12

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003669)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55750 AT1G55750 AT1G55750 AT1G55750 AT1G55750 AT1G55750 AT3G61420 AT3G61420
fragaria_vesca	FvH4_5g28910 FvH4_5g28910 FvH4_5g28910 FvH4_5g28910 FvH4_5g28910 FvH4_5g28910 FvH4_5g28910 FvH4_6g25000
malus_domestica	MD08G1246300.v1.1 MD15G1441400.v1.1
prunus_persica	Prupe.1G583500_v2.0.a1 Prupe.1G583500_v2.0.a1 Prupe.1G583500_v2.0.a1 Prupe.1G583500_v2.0.a1
pyrus_communis	pycom08g21370 pycom15g38940
rosa_chinensis	RchiOBHm_Chr7g0221221
rosa_laevigata	RLG00000002169
rosa_multiflora	Rmu_sc0000324.1_g000015
rosa_roxburghii	Rroxscaffold_3G00238550
rosa_rugosa	Rorug07G0201400 Rorug07G0201500
rosa_samantha	Rh7AG340400 Rh7BG331500 Rh7CG358300 Rh7DG339100
rosa_wichuraiana	Rw7G028970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1683
AccI	GTMKAC	2 cut(s)	146, 1574
AciI	CCGC	4 cut(s)	62, 791, 1105, 1730
AclWI	GGATC	8 cut(s)	131, 201, 214, 878, 953, 1070, 1223, 1236
AcoI	YGGCCR	1 cut(s)	1706
AcsI	RAATTY	3 cut(s)	571, 755, 1387
AcuI	CTGAAG	1 cut(s)	1643
AfaI	GTAC	3 cut(s)	541, 769, 1453
AfiI	CCNNNNNNNGG	5 cut(s)	163, 328, 941, 1484, 1687
AgsI	TTSAA	8 cut(s)	127, 576, 698, 1046, 1348, 1384, 1550, 1739
AhlI	ACTAGT	1 cut(s)	700
AjiI	CACGTC	1 cut(s)	144
AjnI	CCWGG	4 cut(s)	79, 88, 684, 1686
Alw21I	GWGCWC	2 cut(s)	49, 414
Alw26I	GTCTC	2 cut(s)	1138, 1230
Alw44I	GTGCAC	1 cut(s)	45
AlwI	GGATC	8 cut(s)	131, 201, 214, 878, 953, 1070, 1223, 1236
AlwNI	CAGNNNCTG	3 cut(s)	341, 494, 800
Ama87I	CYCGRG	2 cut(s)	1233, 1479
AoxI	GGCC	1 cut(s)	1706
ApaLI	GTGCAC	1 cut(s)	45
ApeKI	GCWGC	9 cut(s)	237, 332, 341, 398, 782, 800, 1314, 1528, 1769
ApoI	RAATTY	3 cut(s)	571, 755, 1387
Asp700I	GAANNNNTTC	1 cut(s)	759
AsuC2I	CCSGG	1 cut(s)	302
AsuHPI	GGTGA	2 cut(s)	49, 674
AvaI	CYCGRG	2 cut(s)	1233, 1479
BaeGI	GKGCMC	2 cut(s)	49, 708
BamHI	GGATCC	2 cut(s)	206, 1228
BanI	GGYRCC	1 cut(s)	1683
BanII	GRGCYC	1 cut(s)	414
BbsI	GAAGAC	1 cut(s)	441
Bbv12I	GWGCWC	2 cut(s)	49, 414
BbvCI	CCTCAGC	1 cut(s)	804
BbvI	GCAGC	9 cut(s)	249, 319, 328, 410, 769, 787, 1326, 1515, 1756
BccI	CCATC	5 cut(s)	359, 521, 599, 950, 1055
BceAI	ACGGC	1 cut(s)	522
BciT130I	CCWGG	4 cut(s)	81, 90, 686, 1688
BclI	TGATCA	1 cut(s)	937
BcnI	CCSGG	1 cut(s)	302
BcoDI	GTCTC	2 cut(s)	1138, 1230
BcuI	ACTAGT	1 cut(s)	700
BfaI	CTAG	4 cut(s)	510, 701, 1449, 1655
BfmI	CTRYAG	3 cut(s)	333, 446, 980
BglII	AGATCT	1 cut(s)	657
Bme1390I	CCNGG	5 cut(s)	81, 90, 302, 686, 1688
BmeT110I	CYCGRG	2 cut(s)	1233, 1479
BmgBI	CACGTC	1 cut(s)	144
BmiI	GGNNCC	5 cut(s)	208, 1119, 1230, 1342, 1685
BmrFI	CCNGG	5 cut(s)	81, 90, 302, 686, 1688
BmrI	ACTGGG	1 cut(s)	702
BmsI	GCATC	6 cut(s)	49, 1540, 1618, 1717, 1723, 1773
BmuI	ACTGGG	1 cut(s)	702
BpiI	GAAGAC	1 cut(s)	441
BpmI	CTGGAG	1 cut(s)	624
Bpu10I	CCTNAGC	1 cut(s)	804
BpuMI	CCSGG	1 cut(s)	302
BsaJI	CCNNGG	4 cut(s)	79, 198, 910, 1478
Bsc4I	CCNNNNNNNGG	5 cut(s)	163, 328, 941, 1484, 1687
Bse1I	ACTGG	2 cut(s)	708, 822
Bse3DI	GCAATG	2 cut(s)	322, 374
BseBI	CCWGG	4 cut(s)	81, 90, 686, 1688
BseDI	CCNNGG	4 cut(s)	79, 198, 910, 1478
BseGI	GGATG	9 cut(s)	64, 618, 814, 844, 922, 1170, 1201, 1285, 1633
BseLI	CCNNNNNNNGG	5 cut(s)	163, 328, 941, 1484, 1687
BseMI	GCAATG	2 cut(s)	322, 374
BseMII	CTCAG	7 cut(s)	247, 406, 486, 795, 1317, 1451, 1550
BseNI	ACTGG	2 cut(s)	708, 822
BseRI	GAGGAG	1 cut(s)	1673
BseSI	GKGCMC	2 cut(s)	49, 708
BseXI	GCAGC	9 cut(s)	249, 319, 328, 410, 769, 787, 1326, 1515, 1756
BsgI	GTGCAG	2 cut(s)	417, 1157
BshFI	GGCC	1 cut(s)	1708
BshNI	GGYRCC	1 cut(s)	1683
BsiHKAI	GWGCWC	2 cut(s)	49, 414
BsiHKCI	CYCGRG	2 cut(s)	1233, 1479
BsiSI	CCGG	2 cut(s)	301, 1681
BslI	CCNNNNNNNGG	5 cut(s)	163, 328, 941, 1484, 1687
BsmAI	GTCTC	2 cut(s)	1138, 1230
BsmI	GAATGC	2 cut(s)	84, 302
BsnI	GGCC	1 cut(s)	1708
BsoBI	CYCGRG	2 cut(s)	1233, 1479
Bsp1286I	GDGCHC	3 cut(s)	49, 414, 708
BspACI	CCGC	4 cut(s)	62, 791, 1105, 1730
BspANI	GGCC	1 cut(s)	1708
BspCNI	CTCAG	7 cut(s)	246, 405, 487, 796, 1318, 1450, 1551
BspHI	TCATGA	1 cut(s)	292
BspLI	GGNNCC	5 cut(s)	208, 1119, 1230, 1342, 1685
BspMAI	CTGCAG	2 cut(s)	337, 450
BspPI	GGATC	8 cut(s)	131, 201, 214, 878, 953, 1070, 1223, 1236
BspT107I	GGYRCC	1 cut(s)	1683
BsrDI	GCAATG	2 cut(s)	322, 374
BsrI	ACTGG	2 cut(s)	708, 822
BssECI	CCNNGG	4 cut(s)	79, 198, 910, 1478
BssT1I	CCWWGG	2 cut(s)	198, 910
Bst2UI	CCWGG	4 cut(s)	81, 90, 686, 1688
Bst4CI	ACNGT	5 cut(s)	440, 767, 1057, 1396, 1499
BstAPI	GCANNNNNTGC	4 cut(s)	341, 350, 800, 1727
BstC8I	GCNNGC	2 cut(s)	45, 216
BstDEI	CTNAG	9 cut(s)	233, 327, 357, 392, 495, 804, 1326, 1437, 1559
BstENI	CCTNNNNNAGG	1 cut(s)	326
BstF5I	GGATG	9 cut(s)	64, 618, 814, 844, 922, 1170, 1201, 1285, 1633
BstMAI	GTCTC	2 cut(s)	1138, 1230
BstNI	CCWGG	4 cut(s)	81, 90, 686, 1688
BstSCI	CCNGG	5 cut(s)	79, 88, 300, 684, 1686
BstSFI	CTRYAG	3 cut(s)	333, 446, 980
BstSLI	GKGCMC	2 cut(s)	49, 708
BstV1I	GCAGC	9 cut(s)	249, 319, 328, 410, 769, 787, 1326, 1515, 1756
BstV2I	GAAGAC	1 cut(s)	441
BstX2I	RGATCY	5 cut(s)	206, 657, 945, 1075, 1228
BstYI	RGATCY	5 cut(s)	206, 657, 945, 1075, 1228
BsuRI	GGCC	1 cut(s)	1708
BtrI	CACGTC	1 cut(s)	144
BtsCI	GGATG	9 cut(s)	64, 618, 814, 844, 922, 1170, 1201, 1285, 1633
BtsIMutI	CAGTG	5 cut(s)	400, 445, 481, 972, 1323
Cac8I	GCNNGC	2 cut(s)	45, 216
CaiI	CAGNNNCTG	3 cut(s)	341, 494, 800
CciI	TCATGA	1 cut(s)	292
CsiI	ACCWGGT	1 cut(s)	1686
Csp6I	GTAC	3 cut(s)	540, 768, 1452
CviAII	CATG	4 cut(s)	293, 941, 1318, 1633
CviQI	GTAC	3 cut(s)	540, 768, 1452
DdeI	CTNAG	9 cut(s)	233, 327, 357, 392, 495, 804, 1326, 1437, 1559
DraI	TTTAAA	3 cut(s)	274, 568, 831
EaeI	YGGCCR	1 cut(s)	1706
Ecl136II	GAGCTC	1 cut(s)	412
Eco130I	CCWWGG	2 cut(s)	198, 910
Eco24I	GRGCYC	1 cut(s)	414
Eco53kI	GAGCTC	1 cut(s)	412
Eco57I	CTGAAG	1 cut(s)	1643
Eco88I	CYCGRG	2 cut(s)	1233, 1479
EcoICRI	GAGCTC	1 cut(s)	412
EcoNI	CCTNNNNNAGG	1 cut(s)	326
EcoRI	GAATTC	1 cut(s)	755
EcoRII	CCWGG	4 cut(s)	79, 88, 684, 1686
EcoT14I	CCWWGG	2 cut(s)	198, 910
EcoT22I	ATGCAT	1 cut(s)	1716
EcoT38I	GRGCYC	1 cut(s)	414
ErhI	CCWWGG	2 cut(s)	198, 910
FaeI	CATG	4 cut(s)	296, 944, 1321, 1636
FatI	CATG	4 cut(s)	292, 940, 1317, 1632
FauNDI	CATATG	2 cut(s)	459, 1336
FbaI	TGATCA	1 cut(s)	937
FblI	GTMKAC	2 cut(s)	146, 1574
FokI	GGATG	9 cut(s)	71, 625, 821, 851, 929, 1177, 1188, 1292, 1640
FriOI	GRGCYC	1 cut(s)	414
FspBI	CTAG	4 cut(s)	510, 701, 1449, 1655
GsuI	CTGGAG	1 cut(s)	624
HaeIII	GGCC	1 cut(s)	1708
HapII	CCGG	2 cut(s)	301, 1681
Hin1II	CATG	4 cut(s)	296, 944, 1321, 1636
HindIII	AAGCTT	1 cut(s)	1272
HinfI	GANTC	8 cut(s)	491, 871, 1283, 1352, 1435, 1474, 1670, 1748
HpaII	CCGG	2 cut(s)	301, 1681
HphI	GGTGA	2 cut(s)	49, 674
Hpy166II	GTNNAC	5 cut(s)	47, 147, 443, 682, 1575
Hpy8I	GTNNAC	5 cut(s)	47, 147, 443, 682, 1575
Hpy99I	CGWCG	3 cut(s)	25, 28, 879
HpyAV	CCTTC	5 cut(s)	464, 770, 1193, 1342, 1618
HpyCH4III	ACNGT	5 cut(s)	440, 767, 1057, 1396, 1499
HpyCH4IV	ACGT	2 cut(s)	143, 626
HpyF3I	CTNAG	9 cut(s)	233, 327, 357, 392, 495, 804, 1326, 1437, 1559
HpySE526I	ACGT	2 cut(s)	143, 626
Hsp92II	CATG	4 cut(s)	296, 944, 1321, 1636
Ksp22I	TGATCA	1 cut(s)	937
LmnI	GCTCC	5 cut(s)	358, 409, 417, 1117, 1346
Lsp1109I	GCAGC	9 cut(s)	249, 319, 328, 410, 769, 787, 1326, 1515, 1756
LweI	GCATC	6 cut(s)	49, 1540, 1618, 1717, 1723, 1773
MabI	ACCWGGT	1 cut(s)	1686
MaeI	CTAG	4 cut(s)	510, 701, 1449, 1655
MaeII	ACGT	2 cut(s)	143, 626
MaeIII	GTNAC	5 cut(s)	185, 257, 370, 622, 1635
MboII	GAAGA	8 cut(s)	281, 446, 526, 880, 923, 1034, 1062, 1085
MfeI	CAATTG	1 cut(s)	786
MflI	RGATCY	5 cut(s)	206, 657, 945, 1075, 1228
MhlI	GDGCHC	3 cut(s)	49, 414, 708
MlyI	GAGTC	2 cut(s)	1361, 1429
MmeI	TCCRAC	2 cut(s)	590, 911
Mph1103I	ATGCAT	1 cut(s)	1716
MroXI	GAANNNNTTC	1 cut(s)	759
MspA1I	CMGCKG	1 cut(s)	677
MspI	CCGG	2 cut(s)	301, 1681
MspR9I	CCNGG	5 cut(s)	81, 90, 302, 686, 1688
MunI	CAATTG	1 cut(s)	786
Mva1269I	GAATGC	2 cut(s)	84, 302
MvaI	CCWGG	4 cut(s)	81, 90, 686, 1688
NciI	CCSGG	1 cut(s)	302
NdeI	CATATG	2 cut(s)	459, 1336
NlaIII	CATG	4 cut(s)	296, 944, 1321, 1636
NlaIV	GGNNCC	5 cut(s)	208, 1119, 1230, 1342, 1685
NmuCI	GTSAC	2 cut(s)	185, 1635
NsiI	ATGCAT	1 cut(s)	1716
PaeR7I	CTCGAG	1 cut(s)	1233
PagI	TCATGA	1 cut(s)	292
PcsI	WCGNNNNNNNCGW	1 cut(s)	20
PctI	GAATGC	2 cut(s)	84, 302
PdmI	GAANNNNTTC	1 cut(s)	759
PfeI	GAWTC	6 cut(s)	491, 871, 1283, 1474, 1670, 1748
PleI	GAGTC	2 cut(s)	1360, 1429
PpsI	GAGTC	2 cut(s)	1360, 1429
Psp124BI	GAGCTC	1 cut(s)	414
Psp6I	CCWGG	4 cut(s)	79, 88, 684, 1686
PspGI	CCWGG	4 cut(s)	79, 88, 684, 1686
PspN4I	GGNNCC	5 cut(s)	208, 1119, 1230, 1342, 1685
PstI	CTGCAG	2 cut(s)	337, 450
PstNI	CAGNNNCTG	3 cut(s)	341, 494, 800
PsuI	RGATCY	5 cut(s)	206, 657, 945, 1075, 1228
PvuII	CAGCTG	1 cut(s)	677
RsaI	GTAC	3 cut(s)	541, 769, 1453
RsaNI	GTAC	3 cut(s)	540, 768, 1452
SacI	GAGCTC	1 cut(s)	414
SchI	GAGTC	2 cut(s)	1361, 1429
ScrFI	CCNGG	5 cut(s)	81, 90, 302, 686, 1688
SduI	GDGCHC	3 cut(s)	49, 414, 708
SexAI	ACCWGGT	1 cut(s)	1686
SfaNI	GCATC	6 cut(s)	49, 1540, 1618, 1717, 1723, 1773
SfcI	CTRYAG	3 cut(s)	333, 446, 980
Sfr274I	CTCGAG	1 cut(s)	1233
SlaI	CTCGAG	1 cut(s)	1233
SmlI	CTYRAG	1 cut(s)	1233
SmoI	CTYRAG	1 cut(s)	1233
SpeI	ACTAGT	1 cut(s)	700
SsiI	CCGC	4 cut(s)	62, 791, 1105, 1730
SspI	AATATT	3 cut(s)	743, 846, 1444
SspMI	CTAG	4 cut(s)	510, 701, 1449, 1655
SstI	GAGCTC	1 cut(s)	414
StyD4I	CCNGG	5 cut(s)	79, 88, 300, 684, 1686
StyI	CCWWGG	2 cut(s)	198, 910
TaaI	ACNGT	5 cut(s)	440, 767, 1057, 1396, 1499
TaiI	ACGT	2 cut(s)	146, 629
TaqI	TCGA	6 cut(s)	26, 874, 1186, 1234, 1538, 1648
TauI	GCSGC	3 cut(s)	65, 794, 1733
TfiI	GAWTC	6 cut(s)	491, 871, 1283, 1474, 1670, 1748
TscAI	CASTG	5 cut(s)	400, 445, 481, 979, 1330
TseFI	GTSAC	2 cut(s)	185, 1635
TseI	GCWGC	9 cut(s)	237, 332, 341, 398, 782, 800, 1314, 1528, 1769
Tsp45I	GTSAC	2 cut(s)	185, 1635
TspGWI	ACGGA	1 cut(s)	404
TspRI	CASTG	5 cut(s)	400, 445, 481, 979, 1330
VneI	GTGCAC	1 cut(s)	45
XagI	CCTNNNNNAGG	1 cut(s)	326
XapI	RAATTY	3 cut(s)	571, 755, 1387
XhoI	CTCGAG	1 cut(s)	1233
XmiI	GTMKAC	2 cut(s)	146, 1574
XmnI	GAANNNNTTC	1 cut(s)	759
XspI	CTAG	4 cut(s)	510, 701, 1449, 1655
Zsp2I	ATGCAT	1 cut(s)	1716

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.