Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0003669

Physical Location & Seq

Genomic Coordinates Forward (+)

17514399 .. 17527615

Gene Structure 13217 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0201500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4914 bp

ATGGAGATTCTTGTTGCAATAGCTGGAAAACTTGCCGAGTACACGGTTGAACCTATTGGACGCCAAGTGGGTTATCTAGCTCACTACAAAAGCAACCATCAAAAGCTGCAGACCAAATTGGAGGACTTAAGTGCTGCCAGAGATGAGGTTCAACATAAGGTTGATCAAGTTGAGAGAGGAGGTAAAAGAATCGAGACTACTGTTCTGAACTGGAAGACAAGAGTGGATGCAATCATCACAGAGGCAGAGGAATTCCTGAGAGACGGCCGCCATGCACAGACAGAGTGTCTTCATGGTTTTTGTCCTCATTTGGTGCTCCGTTATCAACTAAGCAAGAAATCAACAAAATTGGTGGAGGAGATTGATGAACTTTATGGAAAAAAGGAGTTCTCCAACTTTGCATATGATGTTCTTCCACAAGATTTATGTCTCTTATCTACCGGGCATTACCATGCATTTGATTCAAGAAATTCAACTATGATGAAAGTCATGGATGAACTTAGAAATCCTAATACAGAAATGATTCTGGTGTGGGGAATCGGTGGAGTAGGGAAGACCACACTTGTGGAAGAAGTTCTTAGGAAAGCTAAGGACGACAACTTATTTGATGATGTGGTGATGGTGAGAGATGTTAAAGAGAACCCAGACCTTGAAAAAATCCAAAAAGAAATTGCCACAAAGTTAGGTATGAGCATCAATGAAAATCAGACTCTAGCTGAAAGAGCCCCCTCTCTATGTACTAGGATAAAAGAAAAAAGGACTCTTGTAGTTTTGGATGATGTTTGGGAAAAAGTTGATCTGGTGGCTGTCGGACTTCCCCGTCTGGTAACTTGTAAAGTAGTGCTGACATGTAGAAGCCGAAAGGAATTGTCGTTGGATATGCGTACACAAACATATATTTCACTTGATGTTTTAAATGAACAGGAAGCATGGAGTTTGTTTGAGAAGATGGCTGGTGATGTTGTCAAAGATAGCCGCATACGAAGCAAAGCAATGAAAGTAGCCAAAAAATGTGGAGGTTTGCCGGTTTTGATTGTTACGGTTGCAAGAGCTCTAAAAGACAGAAACGGGTTGCATCATTGGAATGTCACCTTGAGACGTCTTGGAGGCTTTGACGACAAAGAATTGACGGAAAAAGCATACTTGGCTCTGGAGTGGAGTTACGATCAGTTGGAAGATAAAGAGCTTAAGCCTTTGTTCTTGCTTTGTGGGATTATTGCAGTTAAGAACCACATTTATTTGGCAGACTTGTTGAAATATGGTTTTGGTTTGGATTTGATTAAAAATGTCGATACAATAGAAGATGCACGAGATGCATTGTTTACACTTGTTGACAAGCTTAAAGATTCTTGCTTGTTGCTGGACAGTGATATCAATATGCATGTTAGAATGCATGATCTTGTATGTGAGGTTGCTACACAGATTGCATCCAGAGATCAACATGTCTTATCAGTTGAGTACGAAGGTGAACTAAAAGAATGGCCTGATAAAGATTTCTTTGAAAGGTGCAGTAAGATGTCTTTAAAATCCGGCCATGTTCCCATGCTTTCCGGAGTACCTTGGAAATGTCCGAAACTAGAAATGTTTCATTTGTATAGTACAGGCCTGGGTGTTGCATCGGTGGAATTCCCTGCCAGCTTTTTTAGTGAAATGGAGATACTGAAAGTGTTGGGTTTAACAAAAGTGTGTATGCTGTCACTACCCTCATCTCTTCAGTTCTTGAAGAATCTTCAGACGCTGTGTTTAGAAGAATGCCTGCTGGAGGAAGAGAATCAAGGGCTAGCTCTGGTTGGACATCTATGTAACTTAGAGATTCTTAGCTTTTCGAGGTCCAACATTAAAGAGCTACCCGAAGAAATAGGGCAATTGACTCGACTGAGATGGTTGGATTTGAGTGATTGTAAACTTCAAGTGATTTCACCTGGTGTTATTTCAAGGTTGGAAAGACTAGAAGACTTATCACTGAGAAATGTCTTTCTCAAATGGAAGGCTAAAAGGGGAGGAAGGAATGCTAGTCTTTTAGAGCTGAAAGACTTGTCTCAACTAACTGCATTGGAGATATATATTCGAGATGCAAATACTCTTCCATCAAACTTGTTCTCCAGTAAGTTGGTAAGATACCAAATATATATTGGCGATGTTTGGAAGAGCAGATATAGTTGGGAGAAGACTATGGCTGAAATGACACTCAACACGCTAAAGCTGAAGCTCGCTTCCAGCAATGAATTGGACCACGGTGTCAATACATTGTTGAAGAGATCTGAAGATTTGTGTTTGGATGGAACAGAGAGTGTTAATAGTATTATGCGTCAATTAGATGTTGAAGATTTTCAGCAACTGATACACCTCCGTCTCCAAAACAGTACTGTTGATTTCACCTATATCATCAACAAGAAGGTTGTGTTCCCCAACTTAACAACCTTGAGCATATGGAACTGTGATCATCTAAGTTTCTTGTTCTCATTTTCTATGGCTAAAAGTTTGGTGGAGCTCAAGCATCTTGAAGTATCCACATGTGCAATAATGGAAGAGATAGTATCAACAAGAGATCAATCCAGTGGAGAAAATATCGATATCATGTTTTGTAAGTTGGAAAGTATAACACTGCATGAACTCCCAAACCTTGCTAGATTCTTTACTGATAGTTGTATTGAACTTCCTCCCGTGAAGAAATTTGATACGAAACAGGGGGCATCCATTTTTGATCCTATGAGTAGTGAAAAGTTTGATGTCGAGAGTGGGACTGTTGTACGAACTTGTCTTTTTGACGCACAGGTAAGATTTCCGAGCTTGGAGAAATTGAAAATTGAAGGGCTAATGAAGTTGCAGACAATATGGAACAACCAACTTGTTGATCAAGACTATTTTCCAAGACTCAGAGAAGTTAATATTTCCCAATGCAACAGTCTAGTGAATGTGTTTGTGCCTAGAATGATAGGAAGACTGAATGTGCTAGAAACCTTATACATACGCGATTGCGACTCACTAGAACTGGTATTCGAAGTCGGAGGAACTGATGTTGAAGAAACACCTACAGACACATCATCATCCTCCCATCAGTTGAGACCTTTTTACTGTCAAAGTCTTGGTTCAGTATGCATACGCAGGTGTAAAAATCTGAAAAAGATCTTCCCGACCTCAGTAGCCAGAGGTCTCCAGAAGCTAAATTCGCTGGTTGTGGATGAGTGTGATGAAGTGGAGGAAATCATTGTTGGTGAACAAGGATTAGAAACAGAGTCTCCTCAGTTCGTGTTCCCAAAAGTAACAAGTGTGCAATTTTCTCGTATGCCCAAACTGAGTAGGTTCTATCCAGGGATTCATGCTTCCACGTGGCCGTTACTCAGTTGTTTGACAATTTGTGGATGTGGTGAAGTGAAGATGTTTGCCGCAGAATTTCCAATTTCTTCAGAAAGACATGAGCCGAACCCTGAAATTGAACAATCTCTCTTTCTATTTGACAAGGATTCATTTCCCAACTTGGAAGACTTAACCCTGAAAGTTGCGGGTGAGATTTGGTTCGGTCCATCATCCCCAGCACAGTTGTTTCCCAAATTAAAAAGTATTCTGTTTCTCAAGTGGGCCCCGTTCTCCAACCAAGCCGCAATACATTTCCTTGAGAAATTACCGAATCTTGAGAAGATACAAGTGAGCTTCGATCATGTTTCCACGGAGATACATGCACTTGGGACCCTCTCAACGGTAAAAACCTTGATGGTCCTTGACATGCCCAAGCTCTTGCAATTAGGGAATGGGAACTCTCAAACAGCGGGCCCACTTTTCCCAAACTTGGACACTCTTTATGTGTTTTTCTGTCATAGGTTAAAGAGTACTGTAGAGTCATCGGCAGCAATATCCTTCCGCAATCTAACAACTCTGAGTGTATTGCATTGTGATGGATTGGAATACTTGACAAGTTATTCCGTAGCTAAAAGCTTAATGCAACTGACAACACTGGAAGTGAACAATTGTCGAAGCCTGAGAGCAATTATAGGGGCAAGCAACGAAGATGATCATGATACTTGTGAGATTGCTTTCACCCGGTTGCAACATTTGTCACTTTATCGTCTACCAAGCCTGCAAGGCTTTTGCTCGGGAAATTGCATTGTCAGACTCCCATCCTCAACACAGTTACTTGTCGATTTCTGCGGGATTGAGTTGAAGATTTCTTCTCAAGGGGTCCTGCTCAGTAATCCAAAAACCACAAATAGCAGAGGACGATGTTGGCACGACGGACGATGGCGTTTCATGATCCCCAGGGATTCCATTGAAGGTGCATTTGAGATGGCGTGGTTATTAATTATCTCTTCTATGATTGTAATATGGATAGCTTTTCTGTTCAAAATTCTCCATGGTTCATACTCACCTTCCAAGGGTGCTTTCGTGAATGATTCTAATAATGGTGGCAGTGCCAAGAAGAGAAATGTGTTGCTGGTTGTTGTCCACCCAGATGATGAGTCCATTGTATCAAATTTACAAGCACAAAATTTGGACCTATTCATGGAGATAGTTAAGGTGTCCAACTCAGTAATCCAAAACCAGAGAGTACAAATAGCAGAGGAGGCAGATGATAATGATGATGGAGAAAAAGAAAAAGATGCCGAGTGTTGGCACGATAAGCCTGCAAGCAAGCTCAACGAAGCAAGTCCAGAAGGCCGGTGTAAGATTGATCTGCCGCTGCGTATTTGTGAAATGCTTGGGATGCTTACCATGAAGAAAGAAGAGGCATATAGTGGATGCCCTGTAATGTCACTACGTCAGGGAGGTCAAAGCATTTTATGGGGCGAAATGATGATGGGATCATGGGGACTGGGGAGAGTGTCGAAGCTAAGTGCTGCGGTTGGGCTGATTGGTGCAAGGCAGAGAACACAGAGGCTACTTGAATATTTAGAAGATCAGTTGGTTAAAGCTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0000439 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005675 GO:0006139 GO:0006259 GO:0006281 GO:0006351 GO:0006355 GO:0006357 GO:0006360 GO:0006366 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016310 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0030880 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032806 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070816 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097659 GO:1901360 GO:1901362 GO:1901564 GO:1901576 GO:1902494 GO:1902554 GO:1902911 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03022 ko03420 ko05203 map03022 map03420 map05203

Pfam Domains

Protein Families

BSD PH_TFIIH

Protein Analysis

1637

Amino Acids

185.19

Weight (kDa)

5.74

Isoelectric Point (pI)

43.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NB-ARC	PF00931	158 - 319	5e-28	NB-ARC domain
LRR_14	PF23598	597 - 866	2.5e-12	Leucine-rich repeat region
LRR_8	PF13855	602 - 657	1.6e-06	Leucine rich repeat
LRR_RPS2	PF23247	797 - 877	1.3e-11	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	928 - 994	2.8e-12	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	931 - 1013	5.3e-14	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	1024 - 1123	2.7e-09	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	1169 - 1298	3.9e-08	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	1283 - 1365	7.5e-11	Plant disease resistance protein RPS2-like, leucine-rich repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003669)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55750 AT1G55750 AT1G55750 AT1G55750 AT1G55750 AT1G55750 AT3G61420 AT3G61420
fragaria_vesca	FvH4_5g28910 FvH4_5g28910 FvH4_5g28910 FvH4_5g28910 FvH4_5g28910 FvH4_5g28910 FvH4_5g28910 FvH4_6g25000
malus_domestica	MD08G1246300.v1.1 MD15G1441400.v1.1
prunus_persica	Prupe.1G583500_v2.0.a1 Prupe.1G583500_v2.0.a1 Prupe.1G583500_v2.0.a1 Prupe.1G583500_v2.0.a1
pyrus_communis	pycom08g21370 pycom15g38940
rosa_chinensis	RchiOBHm_Chr7g0221221
rosa_laevigata	RLG00000002169
rosa_multiflora	Rmu_sc0000324.1_g000015
rosa_roxburghii	Rroxscaffold_3G00238550
rosa_rugosa	Rorug07G0201400 Rorug07G0201500
rosa_samantha	Rh7AG340400 Rh7BG331500 Rh7CG358300 Rh7DG339100
rosa_wichuraiana	Rw7G028970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3096
AasI	GACNNNNNNGTC	2 cut(s)	819, 2237
AatII	GACGTC	1 cut(s)	1102
Acc36I	ACCTGC	1 cut(s)	3096
AccI	GTMKAC	1 cut(s)	4066
AccII	CGCG	1 cut(s)	2973
AccIII	TCCGGA	1 cut(s)	1550
AclWI	GGATC	3 cut(s)	2699, 4243, 4804
AcoI	YGGCCR	3 cut(s)	265, 1531, 3332
AcsI	RAATTY	9 cut(s)	251, 469, 1625, 2672, 3166, 3392, 4341, 4468, 4483
AcuI	CTGAAG	5 cut(s)	1697, 1715, 2225, 2283, 3390
AcvI	CACGTG	1 cut(s)	3330
AcyI	GRCGYC	2 cut(s)	61, 1099
AdeI	CACNNNGTG	1 cut(s)	1925
AfiI	CCNNNNNNNGG	6 cut(s)	1762, 3697, 3787, 4370, 4498, 4605
AflII	CTTAAG	2 cut(s)	127, 1187
AflIII	ACRYGT	3 cut(s)	848, 1441, 2513
AhdI	GACNNNNNGTC	1 cut(s)	285
AjnI	CCWGG	4 cut(s)	1605, 1921, 3310, 4253
AjuI	GAANNNNNNNTTGG	4 cut(s)	3195, 3227, 4361, 4393
AleI	CACNNNNGTG	1 cut(s)	563
Alw21I	GWGCWC	3 cut(s)	318, 1054, 2493
Alw26I	GTCTC	9 cut(s)	188, 255, 434, 1090, 2043, 2357, 3058, 3158, 3243
AlwI	GGATC	3 cut(s)	2699, 4243, 4804
AlwNI	CAGNNNCTG	2 cut(s)	1738, 2338
Ama87I	CYCGRG	1 cut(s)	4090
Aor13HI	TCCGGA	1 cut(s)	1550
AoxI	GGCC	8 cut(s)	265, 1481, 1531, 1603, 3332, 3579, 3769, 4651
ApaI	GGGCCC	2 cut(s)	3583, 3773
ApeKI	GCWGC	5 cut(s)	106, 134, 3845, 4675, 4832
ApoI	RAATTY	9 cut(s)	251, 469, 1625, 2672, 3166, 3392, 4341, 4468, 4483
ArsI	GACNNNNNNTTYG	2 cut(s)	587, 619
AseI	ATTAAT	1 cut(s)	4295
Asp700I	GAANNNNTTC	4 cut(s)	522, 573, 1584, 2328
AsuC2I	CCSGG	2 cut(s)	442, 4039
AsuII	TTCGAA	1 cut(s)	3000
AsuNHI	GCTAGC	1 cut(s)	1780
AvaI	CYCGRG	1 cut(s)	4090
AvaII	GGWCC	7 cut(s)	1830, 2230, 3521, 3687, 3715, 4177, 4489
BaeGI	GKGCMC	2 cut(s)	3583, 3773
BanII	GRGCYC	5 cut(s)	727, 1054, 2493, 3583, 3773
BauI	CACGAG	1 cut(s)	1308
BbrPI	CACGTG	1 cut(s)	3330
BbsI	GAAGAC	7 cut(s)	221, 281, 560, 1959, 2174, 2947, 3489
Bbv12I	GWGCWC	3 cut(s)	318, 1054, 2493
BbvI	GCAGC	5 cut(s)	93, 121, 3857, 4662, 4819
BceAI	ACGGC	2 cut(s)	280, 3319
BcgI	CGANNNNNNTGC	6 cut(s)	1853, 1887, 3263, 3297, 4601, 4635
BciT130I	CCWGG	4 cut(s)	1607, 1923, 3312, 4255
BciVI	GTATCC	1 cut(s)	2518
BclI	TGATCA	4 cut(s)	163, 2440, 2854, 4009
BcnI	CCSGG	2 cut(s)	442, 4039
BcoDI	GTCTC	9 cut(s)	188, 255, 434, 1090, 2043, 2357, 3058, 3158, 3243
BfmI	CTRYAG	3 cut(s)	107, 3033, 3831
BfrI	CTTAAG	2 cut(s)	127, 1187
BfuAI	ACCTGC	1 cut(s)	3096
BfuI	GTATCC	1 cut(s)	2518
BglI	GCCNNNNNGGC	1 cut(s)	4080
BglII	AGATCT	2 cut(s)	2258, 3126
BmcAI	AGTACT	2 cut(s)	2365, 3829
Bme1390I	CCNGG	6 cut(s)	442, 1607, 1923, 3312, 4039, 4255
Bme18I	GGWCC	7 cut(s)	1830, 2230, 3521, 3687, 3715, 4177, 4489
BmeRI	GACNNNNNGTC	1 cut(s)	285
BmeT110I	CYCGRG	1 cut(s)	4090
BmiI	GGNNCC	6 cut(s)	3581, 3582, 3688, 3689, 3771, 4178
BmrFI	CCNGG	6 cut(s)	442, 1607, 1923, 3312, 4039, 4255
BmrI	ACTGGG	1 cut(s)	4817
BmtI	GCTAGC	1 cut(s)	1784
BmuI	ACTGGG	1 cut(s)	4817
BpiI	GAAGAC	7 cut(s)	221, 281, 560, 1959, 2174, 2947, 3489
BpmI	CTGGAG	4 cut(s)	1172, 1781, 2086, 3140
Bpu10I	CCTNAGC	1 cut(s)	588
Bpu14I	TTCGAA	1 cut(s)	3000
BpuEI	CTTGAG	7 cut(s)	1114, 2443, 2477, 3557, 3635, 3653, 4155
BpuMI	CCSGG	2 cut(s)	442, 4039
Bsa29I	ATCGAT	1 cut(s)	2571
BsaAI	YACGTR	1 cut(s)	3330
BsaHI	GRCGYC	2 cut(s)	61, 1099
BsaI	GGTCTC	2 cut(s)	3058, 3158
BsaJI	CCNNGG	9 cut(s)	1559, 1606, 2233, 3311, 3666, 4253, 4254, 4348, 4368
BsaWI	WCCGGW	1 cut(s)	1550
BsaXI	ACNNNNNCTCC	4 cut(s)	2084, 2114, 3223, 3253
Bsc4I	CCNNNNNNNGG	6 cut(s)	1762, 3697, 3787, 4370, 4498, 4605
Bse118I	RCCGGY	2 cut(s)	1024, 4653
Bse1I	ACTGG	6 cut(s)	215, 2103, 2556, 2997, 3957, 4812
Bse3DI	GCAATG	2 cut(s)	999, 2227
BseAI	TCCGGA	1 cut(s)	1550
BseBI	CCWGG	4 cut(s)	1607, 1923, 3312, 4255
BseCI	ATCGAT	1 cut(s)	2571
BseDI	CCNNGG	9 cut(s)	1559, 1606, 2233, 3311, 3666, 4253, 4254, 4348, 4368
BseLI	CCNNNNNNNGG	6 cut(s)	1762, 3697, 3787, 4370, 4498, 4605
BseMI	GCAATG	2 cut(s)	999, 2227
BseNI	ACTGG	6 cut(s)	215, 2103, 2556, 2997, 3957, 4812
BseRI	GAGGAG	4 cut(s)	192, 371, 3231, 4571
BseSI	GKGCMC	2 cut(s)	3583, 3773
BseX3I	CGGCCG	1 cut(s)	265
BseXI	GCAGC	5 cut(s)	93, 121, 3857, 4662, 4819
BseYI	CCCAGC	1 cut(s)	3532
BsgI	GTGCAG	1 cut(s)	1528
Bsh1236I	CGCG	1 cut(s)	2973
Bsh1285I	CGRYCG	1 cut(s)	268
BshFI	GGCC	8 cut(s)	267, 1483, 1533, 1605, 3334, 3581, 3771, 4653
BshVI	ATCGAT	1 cut(s)	2571
BsiEI	CGRYCG	1 cut(s)	268
BsiHKAI	GWGCWC	3 cut(s)	318, 1054, 2493
BsiHKCI	CYCGRG	1 cut(s)	4090
BsiSI	CCGG	6 cut(s)	441, 1025, 1530, 1551, 4039, 4654
BslFI	GGGAC	3 cut(s)	2755, 3700, 4818
BslI	CCNNNNNNNGG	6 cut(s)	1762, 3697, 3787, 4370, 4498, 4605
BsmAI	GTCTC	9 cut(s)	188, 255, 434, 1090, 2043, 2357, 3058, 3158, 3243
BsmBI	CGTCTC	3 cut(s)	255, 1090, 2357
BsmFI	GGGAC	3 cut(s)	2755, 3700, 4818
BsmI	GAATGC	3 cut(s)	1395, 1757, 2014
BsnI	GGCC	8 cut(s)	267, 1483, 1533, 1605, 3334, 3581, 3771, 4653
Bso31I	GGTCTC	2 cut(s)	3058, 3158
BsoBI	CYCGRG	1 cut(s)	4090
Bsp119I	TTCGAA	1 cut(s)	3000
Bsp120I	GGGCCC	2 cut(s)	3579, 3769
Bsp1286I	GDGCHC	6 cut(s)	318, 727, 1054, 2493, 3583, 3773
Bsp13I	TCCGGA	1 cut(s)	1550
Bsp19I	CCATGG	1 cut(s)	4348
BspANI	GGCC	8 cut(s)	267, 1483, 1533, 1605, 3334, 3581, 3771, 4653
BspDI	ATCGAT	1 cut(s)	2571
BspEI	TCCGGA	1 cut(s)	1550
BspFNI	CGCG	1 cut(s)	2973
BspHI	TCATGA	2 cut(s)	4012, 4245
BspLI	GGNNCC	6 cut(s)	3581, 3582, 3688, 3689, 3771, 4178
BspMAI	CTGCAG	1 cut(s)	111
BspMI	ACCTGC	1 cut(s)	3096
BspOI	GCTAGC	1 cut(s)	1784
BspPI	GGATC	3 cut(s)	2699, 4243, 4804
BspQI	GCTCTTC	1 cut(s)	2141
BspT104I	TTCGAA	1 cut(s)	3000
BspTI	CTTAAG	2 cut(s)	127, 1187
BspTNI	GGTCTC	2 cut(s)	3058, 3158
BsrDI	GCAATG	2 cut(s)	999, 2227
BsrFI	RCCGGY	2 cut(s)	1024, 4653
BsrI	ACTGG	6 cut(s)	215, 2103, 2556, 2997, 3957, 4812
BssAI	RCCGGY	2 cut(s)	1024, 4653
BssECI	CCNNGG	9 cut(s)	1559, 1606, 2233, 3311, 3666, 4253, 4254, 4348, 4368
BssNI	GRCGYC	2 cut(s)	61, 1099
BssSI	CACGAG	1 cut(s)	1308
BssT1I	CCWWGG	3 cut(s)	1559, 4348, 4368
Bst2BI	CACGAG	1 cut(s)	1308
Bst2UI	CCWGG	4 cut(s)	1607, 1923, 3312, 4255
Bst6I	CTCTTC	9 cut(s)	1716, 1761, 2088, 2141, 2249, 2523, 4309, 4409, 4713
BstACI	GRCGYC	2 cut(s)	61, 1099
BstAFI	CTTAAG	2 cut(s)	127, 1187
BstAPI	GCANNNNNTGC	1 cut(s)	1313
BstBAI	YACGTR	1 cut(s)	3330
BstBI	TTCGAA	1 cut(s)	3000
BstDSI	CCRYGG	3 cut(s)	2233, 3666, 4348
BstENI	CCTNNNNNAGG	1 cut(s)	1760
BstFNI	CGCG	1 cut(s)	2973
BstMAI	GTCTC	9 cut(s)	188, 255, 434, 1090, 2043, 2357, 3058, 3158, 3243
BstMCI	CGRYCG	1 cut(s)	268
BstMWI	GCNNNNNNNGC	9 cut(s)	722, 984, 1145, 1313, 3169, 4080, 4559, 4615, 4699
BstNI	CCWGG	4 cut(s)	1607, 1923, 3312, 4255
BstNSI	RCATGY	6 cut(s)	852, 1385, 1445, 2517, 3680, 3727
BstSCI	CCNGG	6 cut(s)	440, 1605, 1921, 3310, 4037, 4253
BstSFI	CTRYAG	3 cut(s)	107, 3033, 3831
BstSLI	GKGCMC	2 cut(s)	3583, 3773
BstUI	CGCG	1 cut(s)	2973
BstV1I	GCAGC	5 cut(s)	93, 121, 3857, 4662, 4819
BstV2I	GAAGAC	7 cut(s)	221, 281, 560, 1959, 2174, 2947, 3489
BstX2I	RGATCY	2 cut(s)	2258, 3126
BstXI	CCANNNNNNTGG	2 cut(s)	565, 2110
BstYI	RGATCY	2 cut(s)	2258, 3126
BstZI	CGGCCG	1 cut(s)	265
Bsu15I	ATCGAT	1 cut(s)	2571
BsuI	GTATCC	1 cut(s)	2518
BsuRI	GGCC	8 cut(s)	267, 1483, 1533, 1605, 3334, 3581, 3771, 4653
BsuTUI	ATCGAT	1 cut(s)	2571
BtgI	CCRYGG	3 cut(s)	2233, 3666, 4348
BtgZI	GCGATG	1 cut(s)	2151
BtsI	GCAGTG	2 cut(s)	2603, 4411
BtsIMutI	CAGTG	6 cut(s)	1372, 1961, 2563, 2603, 3950, 4411
BveI	ACCTGC	1 cut(s)	3096
CaiI	CAGNNNCTG	2 cut(s)	1738, 2338
CciI	TCATGA	2 cut(s)	4012, 4245
Cfr10I	RCCGGY	2 cut(s)	1024, 4653
ClaI	ATCGAT	1 cut(s)	2571
CseI	GACGC	4 cut(s)	69, 1744, 2297, 2777
CsiI	ACCWGGT	1 cut(s)	1921
CspCI	CAANNNNNGTGG	4 cut(s)	333, 368, 994, 1029
DraI	TTTAAA	2 cut(s)	915, 1524
DraIII	CACNNNGTG	1 cut(s)	1925
DrdI	GACNNNNNNGTC	2 cut(s)	819, 2237
DriI	GACNNNNNGTC	1 cut(s)	285
DseDI	GACNNNNNNGTC	2 cut(s)	819, 2237
EaeI	YGGCCR	3 cut(s)	265, 1531, 3332
EagI	CGGCCG	1 cut(s)	265
Eam1104I	CTCTTC	9 cut(s)	1716, 1761, 2088, 2141, 2249, 2523, 4309, 4409, 4713
Eam1105I	GACNNNNNGTC	1 cut(s)	285
EarI	CTCTTC	9 cut(s)	1716, 1761, 2088, 2141, 2249, 2523, 4309, 4409, 4713
Ecl136II	GAGCTC	2 cut(s)	1052, 2491
EclXI	CGGCCG	1 cut(s)	265
Eco130I	CCWWGG	3 cut(s)	1559, 4348, 4368
Eco147I	AGGCCT	1 cut(s)	1605
Eco24I	GRGCYC	5 cut(s)	727, 1054, 2493, 3583, 3773
Eco31I	GGTCTC	2 cut(s)	3058, 3158
Eco32I	GATATC	2 cut(s)	1372, 2575
Eco47I	GGWCC	7 cut(s)	1830, 2230, 3521, 3687, 3715, 4177, 4489
Eco52I	CGGCCG	1 cut(s)	265
Eco53kI	GAGCTC	2 cut(s)	1052, 2491
Eco57I	CTGAAG	5 cut(s)	1697, 1715, 2225, 2283, 3390
Eco72I	CACGTG	1 cut(s)	3330
Eco88I	CYCGRG	1 cut(s)	4090
EcoICRI	GAGCTC	2 cut(s)	1052, 2491
EcoNI	CCTNNNNNAGG	1 cut(s)	1760
EcoO109I	RGGNCCY	3 cut(s)	3580, 3687, 4177
EcoRI	GAATTC	2 cut(s)	251, 1625
EcoRII	CCWGG	4 cut(s)	1605, 1921, 3310, 4253
EcoRV	GATATC	2 cut(s)	1372, 2575
EcoT14I	CCWWGG	3 cut(s)	1559, 4348, 4368
EcoT22I	ATGCAT	5 cut(s)	457, 1318, 1383, 1395, 3101
EcoT38I	GRGCYC	5 cut(s)	727, 1054, 2493, 3583, 3773
ErhI	CCWWGG	3 cut(s)	1559, 4348, 4368
Esp3I	CGTCTC	3 cut(s)	255, 1090, 2357
FalI	AAGNNNNNCTT	2 cut(s)	561, 593
FaqI	GGGAC	3 cut(s)	2755, 3700, 4818
FauI	CCCGC	3 cut(s)	3496, 3760, 4139
FauNDI	CATATG	2 cut(s)	403, 2429
FbaI	TGATCA	4 cut(s)	163, 2440, 2854, 4009
FblI	GTMKAC	1 cut(s)	4066
FriOI	GRGCYC	5 cut(s)	727, 1054, 2493, 3583, 3773
GsaI	CCCAGC	1 cut(s)	3536
GsuI	CTGGAG	4 cut(s)	1172, 1781, 2086, 3140
HaeIII	GGCC	8 cut(s)	267, 1483, 1533, 1605, 3334, 3581, 3771, 4653
HapII	CCGG	6 cut(s)	441, 1025, 1530, 1551, 4039, 4654
HgaI	GACGC	4 cut(s)	69, 1744, 2297, 2777
Hin1I	GRCGYC	2 cut(s)	61, 1099
HincII	GTYRAC	1 cut(s)	1333
HindII	GTYRAC	1 cut(s)	1333
HindIII	AAGCTT	2 cut(s)	1337, 3931
HpaII	CCGG	6 cut(s)	441, 1025, 1530, 1551, 4039, 4654
Hpy99I	CGWCG	1 cut(s)	4232
HpyAV	CCTTC	9 cut(s)	1457, 1981, 1998, 2389, 2804, 3865, 4262, 4374, 4643
HpyCH4IV	ACGT	3 cut(s)	1099, 3329, 4753
HpyF10VI	GCNNNNNNNGC	9 cut(s)	722, 984, 1145, 1313, 3169, 4080, 4559, 4615, 4699
HpySE526I	ACGT	3 cut(s)	1099, 3329, 4753
Hsp92I	GRCGYC	2 cut(s)	61, 1099
KflI	GGGWCCC	1 cut(s)	3687
Kpn2I	TCCGGA	1 cut(s)	1550
Ksp22I	TGATCA	4 cut(s)	163, 2440, 2854, 4009
LguI	GCTCTTC	1 cut(s)	2141
LmnI	GCTCC	2 cut(s)	321, 2488
Lsp1109I	GCAGC	5 cut(s)	93, 121, 3857, 4662, 4819
MabI	ACCWGGT	1 cut(s)	1921
MaeII	ACGT	3 cut(s)	1099, 3329, 4753
MfeI	CAATTG	2 cut(s)	1865, 3964
MflI	RGATCY	2 cut(s)	2258, 3126
MhlI	GDGCHC	6 cut(s)	318, 727, 1054, 2493, 3583, 3773
MlyI	GAGTC	9 cut(s)	703, 754, 1864, 2868, 2975, 3245, 3845, 4104, 4463
Mph1103I	ATGCAT	5 cut(s)	457, 1318, 1383, 1395, 3101
MroI	TCCGGA	1 cut(s)	1550
MroXI	GAANNNNTTC	4 cut(s)	522, 573, 1584, 2328
MslI	CAYNNNNRTG	5 cut(s)	450, 563, 1083, 3891, 4446
MspA1I	CMGCKG	2 cut(s)	3767, 4675
MspCI	CTTAAG	2 cut(s)	127, 1187
MspI	CCGG	6 cut(s)	441, 1025, 1530, 1551, 4039, 4654
MspR9I	CCNGG	6 cut(s)	442, 1607, 1923, 3312, 4039, 4255
MunI	CAATTG	2 cut(s)	1865, 3964
Mva1269I	GAATGC	3 cut(s)	1395, 1757, 2014
MvaI	CCWGG	4 cut(s)	1607, 1923, 3312, 4255
MvnI	CGCG	1 cut(s)	2973
MwoI	GCNNNNNNNGC	9 cut(s)	722, 984, 1145, 1313, 3169, 4080, 4559, 4615, 4699
NciI	CCSGG	2 cut(s)	442, 4039
NcoI	CCATGG	1 cut(s)	4348
NdeI	CATATG	2 cut(s)	403, 2429
NheI	GCTAGC	1 cut(s)	1780
NlaIV	GGNNCC	6 cut(s)	3581, 3582, 3688, 3689, 3771, 4178
NmeAIII	GCCGAG	2 cut(s)	61, 4624
NmuCI	GTSAC	4 cut(s)	1087, 1695, 4053, 4746
NsiI	ATGCAT	5 cut(s)	457, 1318, 1383, 1395, 3101
NspI	RCATGY	6 cut(s)	852, 1385, 1445, 2517, 3680, 3727
NspV	TTCGAA	1 cut(s)	3000
OliI	CACNNNNGTG	1 cut(s)	563
PagI	TCATGA	2 cut(s)	4012, 4245
PaqCI	CACCTGC	1 cut(s)	3096
PasI	CCCWGGG	1 cut(s)	4254
PceI	AGGCCT	1 cut(s)	1605
PciI	ACATGT	3 cut(s)	848, 1441, 2513
PciSI	GCTCTTC	1 cut(s)	2141
PctI	GAATGC	3 cut(s)	1395, 1757, 2014
PdmI	GAANNNNTTC	4 cut(s)	522, 573, 1584, 2328
PleI	GAGTC	9 cut(s)	703, 754, 1864, 2868, 2975, 3244, 3844, 4104, 4462
PmaCI	CACGTG	1 cut(s)	3330
PmlI	CACGTG	1 cut(s)	3330
PpsI	GAGTC	9 cut(s)	703, 754, 1864, 2868, 2975, 3244, 3844, 4104, 4462
Ppu21I	YACGTR	1 cut(s)	3330
PpuMI	RGGWCCY	2 cut(s)	3687, 4177
PscI	ACATGT	3 cut(s)	848, 1441, 2513
PshBI	ATTAAT	1 cut(s)	4295
Psp124BI	GAGCTC	2 cut(s)	1054, 2493
Psp5II	RGGWCCY	2 cut(s)	3687, 4177
Psp6I	CCWGG	4 cut(s)	1605, 1921, 3310, 4253
PspCI	CACGTG	1 cut(s)	3330
PspFI	CCCAGC	1 cut(s)	3532
PspGI	CCWGG	4 cut(s)	1605, 1921, 3310, 4253
PspN4I	GGNNCC	6 cut(s)	3581, 3582, 3688, 3689, 3771, 4178
PspOMI	GGGCCC	2 cut(s)	3579, 3769
PspPPI	RGGWCCY	2 cut(s)	3687, 4177
PstI	CTGCAG	1 cut(s)	111
PstNI	CAGNNNCTG	2 cut(s)	1738, 2338
PsuI	RGATCY	2 cut(s)	2258, 3126
RseI	CAYNNNNRTG	5 cut(s)	450, 563, 1083, 3891, 4446
SacI	GAGCTC	2 cut(s)	1054, 2493
SapI	GCTCTTC	1 cut(s)	2141
ScaI	AGTACT	2 cut(s)	2365, 3829
SchI	GAGTC	9 cut(s)	703, 754, 1864, 2868, 2975, 3245, 3845, 4104, 4463
ScrFI	CCNGG	6 cut(s)	442, 1607, 1923, 3312, 4039, 4255
SduI	GDGCHC	6 cut(s)	318, 727, 1054, 2493, 3583, 3773
SexAI	ACCWGGT	1 cut(s)	1921
SfcI	CTRYAG	3 cut(s)	107, 3033, 3831
SfuI	TTCGAA	1 cut(s)	3000
SinI	GGWCC	7 cut(s)	1830, 2230, 3521, 3687, 3715, 4177, 4489
SmiMI	CAYNNNNRTG	5 cut(s)	450, 563, 1083, 3891, 4446
SmlI	CTYRAG	9 cut(s)	127, 1093, 1187, 2422, 2492, 3572, 3614, 3632, 4170
SmoI	CTYRAG	9 cut(s)	127, 1093, 1187, 2422, 2492, 3572, 3614, 3632, 4170
SseBI	AGGCCT	1 cut(s)	1605
SspI	AATATT	2 cut(s)	2890, 4883
SstI	GAGCTC	2 cut(s)	1054, 2493
StuI	AGGCCT	1 cut(s)	1605
StyD4I	CCNGG	6 cut(s)	440, 1605, 1921, 3310, 4037, 4253
StyI	CCWWGG	3 cut(s)	1559, 4348, 4368
TaiI	ACGT	3 cut(s)	1102, 3332, 4756
TaqII	GACCGA	1 cut(s)	3509
TatI	WGTACW	6 cut(s)	39, 737, 1598, 2363, 3827, 4543
TauI	GCSGC	5 cut(s)	270, 978, 3389, 3602, 4675
TscAI	CASTG	6 cut(s)	1372, 1968, 2563, 2610, 3957, 4411
TseFI	GTSAC	4 cut(s)	1087, 1695, 4053, 4746
TseI	GCWGC	5 cut(s)	106, 134, 3845, 4675, 4832
Tsp45I	GTSAC	4 cut(s)	1087, 1695, 4053, 4746
TspGWI	ACGGA	6 cut(s)	308, 1145, 2339, 3683, 3910, 4245
TspRI	CASTG	6 cut(s)	1372, 1968, 2563, 2610, 3957, 4411
Vha464I	CTTAAG	2 cut(s)	127, 1187
VpaK11BI	GGWCC	7 cut(s)	1830, 2230, 3521, 3687, 3715, 4177, 4489
VspI	ATTAAT	1 cut(s)	4295
XagI	CCTNNNNNAGG	1 cut(s)	1760
XapI	RAATTY	9 cut(s)	251, 469, 1625, 2672, 3166, 3392, 4341, 4468, 4483
XceI	RCATGY	6 cut(s)	852, 1385, 1445, 2517, 3680, 3727
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2129, 2224
XmiI	GTMKAC	1 cut(s)	4066
XmnI	GAANNNNTTC	4 cut(s)	522, 573, 1584, 2328
ZraI	GACGTC	1 cut(s)	1100
ZrmI	AGTACT	2 cut(s)	2365, 3829
Zsp2I	ATGCAT	5 cut(s)	457, 1318, 1383, 1395, 3101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.